BMS 74575 User Manual

Download

Page 1

BMS Inverted Biological

Microscope

74575

User Manual

This manual is written for BMS INVERTED BIOLOGICAL. For safety and for exerting

the best performance, making you familiar with the instrument entirely, it is strongly recommended that you read this manual carefully before using the microscope. For the

further reference, please place this manual in a position where nearby the worktable and

fetched easily.

Page 2

Use Notices BMS INVERTED BIOLOGICAL

I、Safety Note

1、 Do not keep the instrument in a direct sunlight,

high temperature or humidity, dusty and easy

shaking environment. Make sure the stage is plane,

Figure 1

Figure 2

horizontal and stable enough.

2、 When moving the microscope, please hold up the

instrument with one hand on the lower side of the eyepiece tube ① , and the other hand on the illumination bracket②.（figure1）

3、 If the bacterium solution or the water splash to the

stage, objective or viewing tube, pull out the power

cord at once, and wipe up the microscope.

Otherwise, the instrument will be damaged.

4、 When working, the lamp house on the top of the

arm③(figure1) will become very hot, be sure there

have enough room around the lamp house (especially the top) for cool.

5、 Before replacing the lamp bulb or fuse, turn the

main switch on the “O”(off) position, then cut off the power. If the lamp is on, or soon after it has been turned off, it is hot and will cause serious burns, please do the replacement after it cool down completely.

★ Specified: the halogen lamp 6V/30W

6、 Earth this instrument to prevent the lightning strike. 7、 Use the supplied power cord, please.

Ⅱ、Maintenance

1、 Use the gauze to wipe the glass parts gently .If removing the fingerprints and oil stains , slightly

dampen gauze with the xylene or the admixture liquid which comparison is 3:7 of the ethanol and

the aether to wipe.

★ Note: the ethanol and the aether are all very combustible, do not put these chemicals near fire

or the possible electricity spark source such as the electronics equipments open ,close operation . Use these chemicals in a well ventilated room as far as possible.

2、 Don’t use organic solvent to wipe the non-optical elements, If you need to clean, use the neutral

detergent, please.

3、 When using, if the microscope is splashed by liquid, cut off the power at once, and wipe up the

moisture.

4、 Do not disassemble any parts of the microscope. That will affect the function or decline the

performance of the microscope.

5、 If haven’t mounted the objective , please cover the dust cap to prevent the dust and the splashed

liquid of the tissue culture entering the inside.

6、 When not using, remember to cover up the microscope with the dust casing. And make sure the

Page 3

lamp cools down enough before you do so.

Ⅲ Safety Symbol

Symbol Meaning

The surface is very hot, not touch by your hands

Before using ,please read the instruction carefully, improper operation will result in bodily

injure or instruction malfunction.

The main switch on

The main switch off

Page 4

1. Components BMS INVERTED BIOLOGICAL

Ocular (eyepiece)

Trinocular viewing tube

(fixed)

Objective Lamp house

z 4X z 10X（PH） z 20X（PH） z 40X

Phase contrast slider

Objective turret (Nosepiece)

Microscope base

Stage (fixed)

Quintuple

z Stage lengthen splint z Glass stage

Figure 1

Illumination bracket

Condenser

Stage inserted plate

(fixed)

Page 5

2．Installation BMS INVERTED BIOLOGICAL

2.1 Installing diagram

The following figure shows the installation sequence of the components. The number in the figure

shows the installation steps.

 Before installing, be sure every components is clean, do not score any parts or glass surface.  Keep well with the supplied hexagon wrench. When changing the components, you will need it

again.

Filter holder Condenser

Ocular(eyepiece)

Color filter

Lamp house

illumination unit

Glass stage

Objectiv

Power cord

Microscope base

Stage lengthen splint

Figure 2

Mechanical ruler

Page 6

BMS INVERTED BIOLOGICAL

2.2 Installing steps

Figure 3

Figure 4

2-2-1 Installing and replacing the lamp （figure3）

 Please use the specified halogen Lamp 6V/30W.

1. Hold to the bulb ①after you wrap it with

gauze or other protection materials, then depress

the plugs 2 into the jack 3 on the lamp house,

ensure the filament and the bolt 4 are in a same

level.

2. Replacing the lamp when using or soon

after

When using, or soon after it is turned off, the

lamp, the lamp house and nearby parts will be

very hot and will cause serious burns. Please turn

the main switch on “O” (off), pull up power

plug, and make sure the bulb, the lamp house

and periphery are all cool. Then, you can do your

replacing.

 Please insert the lamp gently, or it will be damaged

by excessive extrusion.

 Do not touch the Halogen bulb with your hands. It will shorten the service life or cause it to burst. If you leave fingerprints on the surface carelessly, clean it with a dry soft cloth.。

2-2-2 Installing the condenser illumination unit（figure 4）

1. Insert the condenser illumination unit 1 into

the bracket 6 gently, according the figure showed

in the left.

2. Turn the condenser illumination unit at

clockwise about 90°, let the “AS” mark of filter holder③ facing forwards, and keep the screw of

condenser illumination unit and the hole of the

holder in line, then screw down the bolt in the

hole with the supplied hexagon spanner.

Insert BNC connector plugs④ into BNC connector

Page 7

2-2-3 Installing the lamp house (figure 5)

Keep the BNC connector plugs① and the lamp house pin② in line, and keep the bolt③ and the condenser jack④ in line, too. Then push the lamp house into the illumination unit gently until they are against.

Figure 5

Page 8

BMS INVERTED BIOLOGICAL

2-2-4 Installing the objective（Fig. 6 and Fig.7）

1. Turning the coarse fusing knob① like the figure

shows till the nosepiece get to its lowest

position.

★For ensuring the safety of the instruction on transportation, the nosepiece is located in the lowest position and the tension adjustment collar② is adjusted in a appropriate tight tension while leaving the

Figure 6

factory.

2. Screw the lowest magnification objective on to

the turret from the nearside, then turn the turret

clockwise, mount other objectives according the

magnification sequence of low to high.

◎ Mount objective like this way will make the

change of magnification to be very easy in using.

◎ It also can install the objective through the stage opening.

Figure 7

★ Clean the objective regularly, the objective used in

the inversed microscope is very sensitivity about

dust.

★ Do cover all the unused holes with turret dust

caps③, to prevent the dust and contamination

entering inside.

★ When operating, use the low magnification

objective （4X or 10X）to search and focus the

specimen at first, then replace the higher magnifications if necessary.

★ When replace the objective, slowly turning the

nosepiece until you hear “clicked”, that means

the objective enter into the right position—center of the light path.

Page 9

2-2-5

Installing the stage lengthen splint and the mechanical ruler（fig. 8）

Figure 8

◎ Stage lengthen splint can be installed in either side of the stage to enlarge the work surface. But you

can’t install the mechanical ruler together.

◎ Generally , the mechanical ruler will be installed in the right side for comfortable adjustment.

1. Installing the stage lengthen splint

First, Screw the fixed bolt① on to the splint , then mount it on to the stage from right or left below,

screwing down it until it stay hard.

2. Installing the mechanical ruler

Please install the ruler like the way of the stage splint.

Page 10

BMS INVERTED BIOLOGICAL

2-2-6 Installing the stage inserted plate（fig.9）

1. When using the glass stage ①, there is no special require,

you just need to place it in a plane.

2. Install the stage inserted plate on to the stage opening.

◎ Turn the disk, let the V nick to face user, so the recognition

of the objective will become easier.

Figure 9

2-2-7 Installing the eyepiece（fig.10）

1. Remove the cap of the eyepiece tube①.

2. Insert the eyepiece into tube until they are against.

2-2-8 Installing the color filters (fig. 11)

★ Be sure the color filter cools down completely before

you change them. Take down the filter holder①, then install the color filters ②you need.

◎ Mount the color filter downwards like ③ shown, keep it

Figure 10

horizontal through the end, not allow inclined.

★ If the color filter is inclined or not get to the

end④, it will drop possibly.

◎The color filter could be piled on the holder, so you can

install more than one filter according the needs if you can

ensure the whole thickness is less than 11mm.

Figure 11

Page 11

BMS INVERTED BIOLOGICAL

2-2-9 Connecting the power cord（fig.12、13 and 14）

figure12

★ Do not bring the power cord to bear a

powerful stress. When being bent or wrapping, the cable and wires will be broken easily.

1. Turn the main switch① on “O”(off) state

before connecting the power cord.

2. Insert the plugs② into the power jack ③of

the microscope safely.

3. Plug the power cord ④into the power supply

receptacle. Make sure the connection is well.

4. Insert BNC connector plugs⑨ into BNC

connector jack⑧.

★ Do use the supplied power cord all the time.

If lost or damaged, select the same standard cord, please.

★ Connect the power cord correctly, to ensure the instrument is earthen.

2-2-10 Replacing the fuse（fig.12and 13）

Figure 13

Do remember to turn the main switch①

on the state of “O”(off) before replacing the

fuse, and unplug the power cord. Rotate the

fuse⑥ kits out of the holder⑦ by the

“--”type screwdriver, replace a new

fuse, then rotate back to the holder

again.

★ Fuse rating: 250V，500mA.

figure14

Page 12

3. Adjustment BMS INVERTED BIOLOGICAL

Diopter ring

Light path selector lever

Light switch

Tension adjustment collar

Aperture diaphragm adjust lever

Phase center bolt

Main switch Coarse focus knob Fine focus knob

Figure 15

Color filter holder

Phase contrast slider

Page 13

4. Operating the adjustment BMS INVERTED BIOLOGICAL

4-1 Microscope base

Figure 16

Figure 17

Figure 18

4-1-1 Turning on the lamp （fig.16）

Connect the power, turn on the main

switch①(shown on the fig.16)which on the bottom side of the base to “－”(on).

4-1-2 Adjusting the brightness （fig.17）

Turning the brightness adjustment knob

clockwise, the voltage raise, and the brightness

strengthen; whereas turning at the contra direction,

the voltage decline, and the brightness weaken.

◎ Using the lamp in a low voltage

condition, will prolong the service life.

4-1-3 Adjusting the Tension Adjustment Collar（fig.18）

★

The tight tension of the coarse focus knob ②had

already adjusted before leaving factory.

◎ How to adjust the tight tension

Turning the tension adjustment collar ①. while

revolving at the direction which shown by the

arrowhead on the figure, the tight tension of the coarse focus knob② is increasing；and if at the contra

direction ,the tight tension will decline.

If the nosepiece dropped automatically, or the

specimen defocused soon even you focus with the fine focus knob③. It means the coarse focus knob

is too loose, you should screw it down at the

direction shown by the arrowhead in the figure18.

Page 14

BMS INVERTED BIOLOGICAL

4-2 Stage

图 19

图 20

4-2-2 Moving the specimen

4-2-1 Setting the specimen（fig.19and fig.20）

Set the specimen in the center of the stage, please.

★

to obtain the best observe effect, please select the containers, such as culture dish and culture bottle, with the bottom thickness is

1.2mm, and the same thickness is also required by the object slide when it is laid the specimen.

◎using the Φ35mm culture dish

You can lay aΦ35mm culture dish on the stage

directly by using the standard center board① of the stage.

◎ using the mechanical ruler

1. When using the 96bit or 24bit micro-titration

board, please fasten it tightly by the stage

clips②.

2. When fastening other model boards, please use

the following supplied brackets with

mechanical ruler:

● Terasaki bracket③ for Terrasaki board

● Culture dish bracket④ for Φ 35mm

culture dish

● Object slide bracket⑤ for object slide

andΦ54mm culture dish

3. Turning the transverse knob ⑥and lengthways

knob⑦, move the specimen to the required

position. ( Movement Range: 120（width）×78

（Length）mm)

Turn the knob of the mechanical ruler or use your hands directly to move the specimen to the position

you wanted, please.

★ be careful when you replace the objective, please, especially after a short work distance

observation. Not let the objective to touch the stage inserted plate or the culture dish bracket.

Page 15

BMS INVERTED BIOLOGICAL

4-3 the viewing tube

Figure 21

Figure 22

4-3-1 Adjusting the diopter（fig.21）

1. Look into the right ocular by your right eye,

then revolving the coarse focus knob to focus

on the specimen.

2. Then use your left eye to look into the left

ocular. If the image is not sharp, just use the

diopter adjustment ring① to adjust please.

★ There are ±5 diopter in the adjustment

ring. The number which the reticle on the eyepiece holder pointed is your eye’s diopter graduation.

4-3-2 Adjusting the interpupillar distance （fig.22）

When observing with two eyes, hold on the left

and right prism holder, turn around the axis,

adjusting the interpupillar distance until the left and

right fields of view coincide completely.

★ The reticle on the interpupillar distance indicator③, pointed by the spot “.” ②on the eyepiece holder, shows the scale of the interpupillar distance.（fig.21）

The range of the interpupillar distance：

48～75mm。

Page 16

4-3-3 Switching the light path（fig.23）

◎ Pulling out the light path selector lever① by your

Figure 23

thumb, select the light path you needed.

◎ when in the binocular observation, pushing in the

lever until you heard a “clicked” .while in

video or photography, pulling out the lever until

it reached the “clicked” position.

Light path

selector

lever

Pushing in

the lever

until it

reached the

limit

position

Pulling out

the lever

until it

reached the

limit

position

Brightness

proportion

100% used for

binocular

observation

20% used for

binocular observation, and 80% used for video or

photography

application

Binocular

observation

Binocular

observation,

television,

and

micrography

or video can

be operated

simultaneous

Page 17

BMS INVERTED BIOLOGICAL

4-4 Illumination Unit

Figure 24

Figure 25

70-80%

30-20%

4-4-1 Using color filters（fig.24）

◎ Selecting the appropriate color filters

according your need, it became more

effective to observe or photography the

specimen. Especially, we suggest using

the LBD color filter, which can

compensate more neutral colors.

◎ You could pile up a group of color

filters to the filter holder, if you

ensure they are level and the whole

thickness is less than 11mm.

Color

filter

IF550

LBD

Single contrast color filter

（used for the phase contrast

Color temperature transit

color filter（blue）

（used for bright field

meaning

(green)

microscopy）

observation and

microphotography）

Page 18

4-4-2 using the aperture diaphragm（fig.25）

◎ When in the bright field observation, the aperture diaphragm control the numerical

aperture of the illumination system. Only when the numerical aperture of the objective

and the illumination system being matching, you can obtain the higher image resolution

and contrast, and the increased depth of field, too.

● To recognize the aperture diaphragm, you could remove the eyepiece if necessary. ( you

also could insert in the center telescope )then looked into the viewing tube, you might

see a field of view like the figure shown. The proportion could be changed by dialing

the aperture adjustment lever according your need. (①is the image of the aperture

diaphragm , ② is the edge of the objective)

● Generally, when observing the chromatic specimen, you need to set the size of the

condenser aperture diaphragm at 70%～80% of the numerical aperture which marked in the

objective. but if observing the bacterium specimen which not colored, you could turn

the aperture diaphragm lever at the direction of“

”(clockwise).

Page 19

5．Phase contrast viewing BMS INVERTED BIOLOGICAL

5-1 The name of the components

Figure 26

1 2

5-1-1 Phase contrast objective（fig.26）

◎ the optional magnification of the

phase contrast is :10X、20X。

◎ If you want to know how to mount the phase

contrast objective, please see 2-2-4.

You ought to mount it on the turret.

5-1-2 Phase contrast slider（fig.27）

◎ phase centering adjustable slider

● The light ring was centered beforehand,

so it needn’t to adjust in the use

process. If the ring is not in the

center, you could adjust by the

centering bolt.

● The 10X/20X light ring ①is worked with

the 10X,20X phase contrast objective,

while the opening② is used for bright

field .

Figure 27

Page 20

BMS INVERTED BIOLOGICAL

5-2 The installation and use

Figure 28

Figure 29

Figure 30

5-2-1 Installing the phase contrast

slider（fig.28）

1. Keep the slider① face ( the surface which had

character) up towards, then inserted it into the

illumination system from the right to the left

like the figure showed.

2. Every light ring or opening has its own

located position, so you need to move them

until you heard the “clicked” to ensure the ring

or the opening reaches the center of the light

path.

3. When in the phase contrast observation, do

keep the aperture diaphragm adjustment lever② on the position of “O” (wide opening).

5-2-2 The centering ring（fig.29 and fig.30）

★ Usually you needn’t the operation of

centering. If necessary, please accord to the following steps:

1. Place the specimen on the stage and focus it.

2. Take out the eyepiece, replace it with the CT ( the centering telescope),and inserted it into the viewing tube without diopter adjustment.

3. Make sure the matched phase contrast objective and light ring( in the phase contrast slider) have been in the center of the light path.

Page 21

4. Using the CT to look the light ring’s image① and the phase contrast ring’s image②, if the light ring’s image is not sharp, please shifting the CT’s ocular until you can see a clear image of the light ring②.

5. Adjusting the bolts of the two centering holes③ in the phase contrast slider by the screwdriver ③until the light ring center and the phase contrast center are coincided.

6. The 10X and the 20X phase contrast objective use the same light ring on the phase contrast slider. So you need to check the coincidence of the light ring center and the phase contrast center when changing the objective. If having departure, you ought to center again.

★ If the light ring is centering incorrectly, you will fail to obtain the best viewing

effect of the microscopy.

★ After removing or replacing a thick specimen, the light ring and the phase contrast

ring are likely to deviate each other, which will result in a decline of the image contrast. So if happened, please repeat the steps as above.

★ If the container or the cover flip which used to place the specimen is not flat, it

maybe need to repeat the centering steps for obtaining a more contrast effect. Please center the light ring by the phase contrast objective, according to the sequence of low to high magnification.

Page 22

6．Microscope photography and video BMS INVERTED BIOLOGICAL

6-1 Microscope video

6-1-1 selecting the light path（fig.31）

Figure 31

★ just used in the trinocular observation

1. Pulling out the light path selector lever, until

you heard the “clicked”.

★ In the dark specimen observation, you can make

the focus by both eyes at first, then change the

light path.

6-1-2 Installing the video set（fig.32）

1. Loosen the locking bolt① on the trinocular viewing tube, and take out the dust cap②.

2. Remove the dust cover on the both ends of the video accessories③, and revolve the screw

head end into the CCD/CMOS port.

3. Install the accessories into the tri-through port, and screw down the bolt①.

6-1-3 Focus（fig.32）

Doing a binocular observation at 20%

brightness, look the image on the video

or the computer which connected with the

video

CCD

microscope video system when the image

is sharp. If it is not in focus, please

turning the revolving video connected

tube④ until the image is sharp enough.

Page 23

BMS INVERTED BIOLOGICAL

6-2 Microscope photography

camera

6-2-1 selecting the light path

★ just used in the trinocular observation

The operation diagram is shown in 6-1-1，

and the details reference is in 4-3-4。

6-2-2 Installing the photography set

（fig.33）

1. Loosen the locking bolt① on the trinocular

viewing tube, and take out the dust cap②.

2. Install the photography accessories③ into the

tri-through port, and screw down the locking bolts①.

3. Inserted the camera gate which on the digital

photography connected head④ into the

correspond position of the camera set port,

and screw it down clockwise.

5. Plug the digital photo connected head into

the photo tube, then screw down the locking bolts①.

● Before connecting the camera and adapter,

remove the camera lens firstly, then connect

the lens port with the adapter. Pay attention

to the gate type, please.

● To avoid the disturbing from the ocular in the

observation, please place the viewer finder

on the two sides of the microscope when

installing the camera set.

● The camera magnification = objective

magnification ×camera lens magnification

★ When shooting the micrograph, the lens

close will bring an impact in some camera. In order to weaken the impact, and obtain a clear image, you could select a longer time of exposure or decrease the brightness to have some compensation.

★ This explanation is used for NiKon

Single-lens reflex digital camera

Page 24

6-2-3 Focus

Do the binocular observation at 20% brightness, and focus primary. When in microscope

photography, do use the camera viewfinder to focus the specimen. Please refer the user manual of the camera set to obtain the details.

6-2-4 Adjusting the color temperature

◎ When shooting the chromophotograph, please use the sunlight film.

1. Mount the LBD temperature changed color filter on to the color filter bracket.

2. Turn the brightness adjustment knob to the maximal position, so you obtain a sunlight

illumination.

Page 25

7．Technical specifications BMS INVERTED BIOLOGICAL

7-1 Main specifications

Optical

system

Viewing Tube

Eyepiece Wide Field Eyepiece 10X, Linear Visual Field: 22 mm

Nosepiece Backward Quintuple Nosepiece

Objective

Focusing

System

Stage

Mechanical

ruler

Illumination Halogen Lamp 6V30W, Preset Center, Intensity continued Adjustable

Condenser Long working Distance Condenser, Numerical Aperture 0.3, Working Distance 72mm

Operation

environment

Infinite Optical System

Compensation Free Trinocular Tube Inclined at 30;

Division ratio: 20% of Binocular Viewing and 80% of Video Viewing & Micrography

Infinite Long Working Distance Plan Achromatic: 4X、40X Infinite Long Working Distance Plan Phase Contrast: 10X、20X

Coaxial Coarse and Fine Focusing System

Sensitivity and Graduation of Fine Focus: 0.002mm

Movement Range(from the surface focus of stage plate): up 8mm, down 3mm Area: 160（width）×250（Length）mm

Movement Range: 120（width）×78（Length）mm

z Use indoor z Altitude: Maximum 2000 m z Temperature: 5℃~40℃（41°F~109°F） z Maximum Relative Humidity: 80% at 31℃（88°F）, then Fall Linear. z 70% at 34℃（93°F）, 60% at 37℃（104°F）,50% at 40℃（104°F）. z Pollution Degree:2 (refer to IEC60664)

7-2Objective Specifications

TYPE

Infinite

Long

Work in g

Distance

Plan

Achromatic

Objective

Infinite

Long

Work in g

Distance

Plan Phase

Contrast

Objective

MAGNIFICATION

4X 0.1 25.2

40X 0.6 3.2

10X 0.25 11

20X

NUMERICAL

APERTURE

（N.A）

0.4

WORKING

DISTANCE

（mm）

CONJUGATE

DISTANCE

（mm）

∞

FOCUS

DISTANCE

（mm）

45 1.2mm

COVER

SLIP

THICKNESS

─

0.17

Page 26

8．Trouble Shooting BMS INVERTED BIOLOGICAL

Under certain condition, some no-fault factors will bring a reversible influence to the instrument’s

performance. If the problem is happened, please take

you can’t solve the trouble by the supplied methods, please contact with the sales department of our company.

proper measures according to the follow table. If

PROBLEM REASON

I、Optical Part:

1. The illumination

is opening, but the

field of view is dark.

2. The edge of the

field of view has

shadow or the

brightness is

asymmetry

Find dust and

stain in the field of view

4. appear double

image

5. Resolution problems：

z Image is not

z The contrast is

z The detail is

z Don’t obtain

sharp；

not high；

not clear；

the phase

contrast effect

The plug of the lamp holder is

not connected into the

illumination set

The bulb burnt out Change a new lamp 3

The brightness is too low Adjust to a proper position 8

The color filter is piled too

much

No use the appointed lamp bulb

The nosepiece is not in the located position

the color filter is stopped

midway

The phase contrast slider is not

located in the proper position

There are stains on the

specimen

There are stains and dust

on the eyepiece

the size of the aperture

diaphragm is too small

The nosepiece is not in the

center of the light path

the aperture diaphragm in the

view of field is opened too

large or too small

The lens (condenser, objective,

ocular or culture dish)

become dirty

In the phase contrast

observation, the bottom

thickness of the culture dish is

more than 1.2mm.

Use a bright field objective Change to the phase contrast

SOLUTION

Connect them well 3

Minimize the number of the

filters

use the specified halogen Lamp

6V30W

Turn the nosepiece into the

position where you can hear

“clicked”

Insert deeply 5

Turn the slider into the

“clicked” position

Open up the aperture

diaphragm

ensure the nosepiece is turned

into the “clicked” position

adjust the aperture diaphragm

correctly

Clean all

Use a the culture dish whose

bottom thickness is less than

1.2mm

objective

Change a clean specimen

Clean the eyepiece

PAGE

Page 27

6.one side of the

image is unfocused

The condenser ring is not

coincident with the objective

phase ring

The light ring and the phase

contrast kits is not centered

The objective used is not fit

to the phase contrast

observation

When looking at the edge of

the culture dish, the phase

contrast ring and the light ring

is deviated each other

The nosepiece is not in the

center of the light path

The specimen don’t place

properly

The optical performance of the

culture dish bottom is poor

(such as erose figure and soon)

Adjust the condenser ring to

match the objective phase ring

Adjust the bolts to center them 12

Please use the compatible

objective

Moving the culture dish until

you obtain the phase contrast

effect. You also could demount

the slider, and dail the field

diaphragm with the direction of

“

”

Insure the nosepiece is in the

“clicked” position

Place the specimen on the

stage correctly.

Please use a regular culture

dish

Page 28

BMS INVERTED BIOLOGICAL

PROBLEM REASON

II、Mechanical Part:

1.The coarse focus

knob is hard to run

2.The image can’t stay on

the focal plane in the

process of the observation

III.Electric Part:

1 The lamp can’t

light

The bulb burns out in a high frequency

1、 The height of

the brightness is

not enough

2、 The light

glimpse

Ⅳ. Viewing tube

The two eyes’ field

of view is different

Ⅴ. Microscope video

1.the image is

unfocused

There is faintness

around the image

3.The indoor window

or the fluorescence

lamp develop

The tension adjustment

collar is too tight

The tension adjustment

collar is too loose

No power supply

the installation of the bulb is wrong

The bulb burn out Change a new bulb

Not use the specified lamp Use the required lamp

Not use a appointed lamp use a appointed lamp

The brightness adjustment

knob is used wrong The bulb is going to spoil Change the bulb The power cord have a poor

contact

The interpupillar distance is

not correct

The diopter is not right Adjust the diopter 10

Not adapte to the microscope

observation

Focus incorrectly

It is a inherent character of the

achromatic objective

The extra light entered into the

eyepiece and viewfinder is

reflected

SOLUTION

Loose properly

Tighten properly

Check the power cord, and

connect them exactly

Install the bulb correctly

Adjust the brightness

adjustment knob in a correct way

Check the power cord, and

connect them exactly

Adjust the interpupillar

distance

When look into the objective, do

not stare at the specimen but at the

whole field of view, or move the

eyes away to see other things, then

back into the objective

Adjusting the focus system, make the double

reticle and the specimen distinctly to see

The problem is unavoidable if you

used an achromatic objective

Cover up the eyepiece and the

viewfinder of the microscope

illumination system

PAGE