Biotek Synergy HTX Operator's Manual

Operator’s Manual

Multi-Mode Microplate Reader

Synergy

™

HTX



Synergy HTX

Multi-Mode Microplate Reader
Operator’s Guide

July 2014
 2014
Part Number 1341000
Revision A
BioTek Instruments, Inc.

ii | Preface

Notices

BioTek Instruments, Inc.

Highland Park, P.O. Box 998

Winooski, Vermont 05404-0998 USA

All Rights Reserved

© 2014, BioTek® Instruments, Incorporated. No part of this publication may be
reproduced, transcribed, or transmitted in any form, or by any means electronic or
mechanical, including photocopying and recording, for any purpose other than the
purchaser’s use without written permission of BioTek Instruments, Inc.

Trademarks

BioTek® is a registered trademark, and Synergy™, Gen5™, BioCell™, 4-Zone™ and
BioStack™ are trademarks of BioTek Instruments, Inc. Harta™ is a trademark of Harta
Instruments.

Microsoft
Microsoft Corporation in the United States and/or other countries.

All other trademarks are the property of their respective holders.

®

, Windows®, and Excel® are either registered trademarks or trademarks of

Restrictions and Liabilities

Information in this document is subject to change and does not represent a
commitment by BioTek Instruments, Inc. Changes made to the information in this
document will be incorporated in new editions of the publication. No responsibility is
assumed by BioTek for the use or reliability of software or equipment that is not
supplied by BioTek or its affiliated dealers.

BioTek Instruments, Inc.

Contents

Contact Information ........................................................... v

Document Conventions ...................................................... vii

Revision History .............................................................. viii

Intended Use Statement .....................................................ix

Quality Control ..................................................................ix

Warranty and Product Registration .......................................ix

Repackaging and Shipping .................................................. x

Warnings .......................................................................... x

Hazards ............................................................................ x

Precautions ...................................................................... xii

CE Mark ......................................................................... xiii

Electromagnetic Interference and Susceptibility ................... xiv

User Safety ...................................................................... xv

Safety Symbols ............................................................... xvi

Introduction ......................................................................... 1

Synergy HTX Multi-Mode Microplate Reader ........................... 2

Package Contents .............................................................. 3

Optional Accessories .......................................................... 4

Product Support & Service .................................................. 5

Installation ........................................................................... 7

Product Registration ........................................................... 8

1: Unpack and Inspect the Reader ....................................... 8

2: Remove the Shipping Panel ........................................... 10

3: Remove the Microplate Carrier Shipping Screw ................. 11

4: Install the Fluorescence Lamp Assembly .......................... 12

Contents | iii

Customer Service and Sales ............................................ v

Global Service and Support ............................................. v

Directive 2004/108/EC: Electromagnetic Compatibility ..... xiii

Directive 2006/95/EC Low Voltage (Safety) ..................... xiii

Directive 2002/96/EC: Waste Electrical and Electronic

Equipment ................................................................. xiv

Directive 98/79/EC: In Vitro Diagnostics (if labeled for this

use) .......................................................................... xiv

USA FCC CLASS A ....................................................... xiv

Canadian Department of Communications Class A ............ xiv

Technical Assistance Center (TAC) ................................... 5

Returning Instruments for Service/Repair ......................... 5

Applications Support ...................................................... 5

Synergy HTX Operator’s Manual

iv | Preface

Getting Started ................................................................... 31

Preventive Maintenance ..................................................... 51

5: Select an Appropriate Location ....................................... 13

6: Connect the Power Supply............................................. 14

7: Unpack and Inspect the Dispense Module ........................ 15

8: Install the Dispense Module ........................................... 18

Record Syringe Calibration Values .................................. 18

Install the Dispenser .................................................... 18

9: Connect the Host Computer .......................................... 20

10: Install Gen5 Software ................................................. 20

11: Turn on the Reader .................................................... 20

12: Establish Communication ............................................ 21

13: Set the Dispenser Calibration Values ............................. 22

14: Run a System Test ..................................................... 23

15: Test the Injector System ............................................. 24

Operational/Performance Qualification ................................ 26

Repackaging and Shipping Instructions ............................... 26

Key Components ............................................................. 32

Power Switch, Carrier Eject Button, Microplate Carrier ...... 32

Lamp Assembly and Filter Wheel Access ......................... 33

Excitation and Emission Filter Wheels ............................. 34

Installing the Time-Resolved Fluorescence Cartridge ........ 37

Configuring the System for Luminescence Measurements .. 38

The External Dispense Module ....................................... 39

Gen5 Software ................................................................ 41

Viewing/Updating the Filter and Wavelengths Tables ........ 42

Creating Protocols and Experiments ............................... 43

Controlling the Dispense Module .................................... 45

Recommendations for Optimum Performance ....................... 48

Incubation and Partial Plates ......................................... 49

Recommended Maintenance Schedule ................................. 52

Overview ................................................................... 52

Daily Cleaning for the Dispense Module .......................... 52

Recommended Maintenance Schedule ............................ 53

Warnings and Precautions ................................................. 54

Cleaning Exposed Surfaces ................................................ 55

Inspect/Clean Excitation and Emission Filters ....................... 56

Flush/Purge the Fluid Path ................................................ 57

Running a Dispense Protocol (Optional) .............................. 58

Empty/Clean the Tip Priming Trough .................................. 59

Clean the Priming Plate ..................................................... 59

Clean the Internal Components .......................................... 60

BioTek Instruments, Inc.

Contents | v

Required Materials ....................................................... 61

Removing the Reader’s Shroud ...................................... 62

Removing the Internal Tubes and Injector Heads ............. 64

Cleaning the Internal Tubes and Injector Heads ............... 67

Cleaning the Optical Probes .......................................... 68

Cleaning the Reader’s Internal Surface ........................... 76

Reassembling the Components ...................................... 77

Performance Check ...................................................... 79

As-Needed Maintenance ..................................................... 81

Purpose .......................................................................... 82

Required Materials ........................................................... 83

Procedure for Models without Injectors ............................... 84

Routine Procedure for Models with Injectors ........................ 85

Clean Exposed Surfaces ............................................... 85

Decontaminate the Fluid Lines ....................................... 86

Rinse the Fluid Lines .................................................... 87

Clean the Internal Tubing and Injector Heads .................. 87

Clean the Tip Priming Trough and Priming Plate ............... 88

Alternate Procedure for Models with Injectors ...................... 89

Instrument Qualification .................................................... 91

Overview ........................................................................ 92

IQ/OQ/PQ ....................................................................... 92

Recommended Qualification Schedule ................................. 94

System Test .................................................................... 95

Absorbance Plate Test ..................................................... 100

Test Plate Certificates ................................................. 100

Define Absorbance Test Plate Parameters ...................... 100

Run the Absorbance Plate Test ..................................... 101

Results and Troubleshooting Tips .................................. 104

Luminescence Tests ........................................................ 105

Harta Plate Test ......................................................... 106

Gen5 Protocol Reading Parameters ............................... 107

Troubleshooting ......................................................... 109

Absorbance Liquid Tests .................................................. 109

Absorbance Liquid Test 1 ............................................. 110

Absorbance Liquid Test 2 ............................................. 112

Absorbance Liquid Test 3 (optional) .............................. 114

Fluorescence Tests .......................................................... 116

Required Materials ...................................................... 117

Test Solutions ............................................................ 118

Procedure ................................................................. 119

Results Analysis ......................................................... 119

Synergy HTX Operator’s Manual

vi | Preface

Specifications ................................................................... 143

Error Codes ...................................................................... 151

Troubleshooting ......................................................... 120

Pipette Map ............................................................... 121

Gen5 Protocol Reading Parameters ............................... 122

Fluorescence Tests Using Methylumbelliferone ................ 126

Dispense Module Tests .................................................... 131

Required Materials ...................................................... 132

Alternate Test Solutions .............................................. 133

Procedure for Models with Absorbance Capabilities .......... 133

Procedure for Models without Absorbance Capabilities ..... 135

Results Analysis ......................................................... 136

Gen5 Test Protocols for Models with Absorbance Capabilities137

Gen5 Test Protocols for Models without Absorbance

Capabilities ............................................................... 139

Create the Dispense Protocols ...................................... 139

General Specifications ..................................................... 144

Absorbance Specifications ................................................ 145

Fluorescence Specifications .............................................. 147

Luminescence Specifications ............................................. 148

Models with Injectors ...................................................... 149

Error Codes Overview ...................................................... 152

Contact Info: BioTek Service/TAC ................................. 152

Error Codes ................................................................... 153

BioTek Instruments, Inc.

Contact Information | v

Mailing Address:

Winooski, VT 05404

Room 304, Tower D

Phone: [phone]

Phone: [phone]

Email: [e-mail]
Website: www.biotek.com

Email: [e-mail]
Website: www.biotekchina.com.cn

Kocherwaldstrasse 34 D-74177

Germany

50 avenue d'Alsace

France

Phone: [phone]
Fax: [phone]

Phone: [phone]
Fax: [phone]

Email: [e-mail]
Website: www.biotek.de

Email: [e-mail]
Website: www.biotek.fr

BioTek India

BioTek Singapore

Unit 223, Linkway Estate

Mumbai 400064

20 Science Park Road #01-08A

Singapore 117674

Contact Information

Customer Service and Sales

Internet: www.biotek.com

Phone: [phone] (toll-free in the U.S.)

802-655-4740 (outside the U.S.)

Fax: [phone]

Email: [e-mail]

Global Service and Support

BioTek instrument service and repair is available worldwide at one of BioTek's
International Service Centers and in the field at your location. For technical assistance,
contact the Technical Assistance Center (TAC) at BioTek World Headquarters US. To
arrange for service or repair of your instrument, contact the office nearest you.

BioTek World Headquarters US BioTek China

PO Box 998, Highland Park
Winooski, VT 05404-0998
United States
Service Shipping Address:
15 Tigan Street

Outside US: [phone]
Fax: [phone]

BioTek Germany Service Center &
European Coordination Center

Bad Friedrichshall

Ocean International Center
62 Middle 4th East Ring Road
Chaoyang District
Beijing 100025
P.R. China

Fax: [phone]

BioTek Instruments SAS
Bureau de liaison France

68025 Colmar Cedex

New Link Road, Malad West

Synergy HTX Operator’s Manual

Teletech Park

vi | Preface

India

Phone: [phone]
Fax: [phone]

Phone: [phone]
Fax: [phone]

Email: [e-mail]
Website: www.biotek.in

Email: [e-mail]
Website: www.biotek.com

3F, Gyungnam building, 830-48

Seoul, South Korea (135-936)

Zentrum Fanhöfli 8

Switzerland

Phone: [phone]
Fax: [phone]

Phone: [phone]
Fax: [phone]

Email: [e-mail]
Website: www.biotekinstruments.co.kr

Email: [e-mail]
Website: www.biotek.ch

6 Bull Street

United Kingdom

Phone: [phone]
Fax: [phone]

Email: [e-mail]
Website: www.biotek.uk.com

BioTek South Korea BioTek Switzerland

Yeoksam-dong, Gangnam-gu

BioTek United Kingdom (UK)

Potton, Bedfordshire SG19 2NR

6014 Luzern

BioTek Instruments, Inc.



Document Conventions

This icon calls attention to important safety notes.

Document Conventions | vii

Warning!

Caution

Note:

italic

A Warning indicates the potential for bodily harm and
tells you how to avoid the problem.

A Caution indicates potential damage to the instrument
and tells you how to avoid the problem.

Bold text is primarily used for emphasis.

Topics that apply only to specific Synergy HTX models are
preceded by a notice in italics, for example: Applies only to
Synergy HTX models with injectors.

This icon calls attention to important information.

Synergy HTX Operator’s Manual

viii | Preface

Rev

Date

Changes

Revision History

A 7/2014 Initial release

BioTek Instruments, Inc.

Intended Use Statement

• The Synergy HTX is a single-channel absorbance, fluorescence, and luminescence
microplate reader that uses a dual-optics design to perform measurements of
samples in a microplate format. The performance characteristics of the data
reduction software have not been established with any laboratory diagnostic assay.
The user must evaluate this instrument and Gen5 software in conjunction with
their specific assay(s). This evaluation must include the confirmation that
performance characteristics for the specific assay(s) are met.

• If the instrument has an “IVD” label, it may be used for clinical and non-clinical
purposes, including research and development. If there is no such label, the
instrument may be used only for research and development or other non-clinical
purposes.

Quality Control

Intended Use Statement | ix

It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for the test
to be conducted. Failure to conduct Quality Control checks could result in erroneous test
data.

Warranty and Product Registration

Take a moment to review the Warranty information that shipped with your product.
Please also register your product with BioTek to ensure that you receive important
information and updates about the product(s) you have purchased. You can register online
through the Customer Resource Center at www.biotek.com or by calling 888/451-5171 or
802/655-4740.

Synergy HTX Operator’s Manual

x | Preface

Operate the instrument on a level, stable surface away from excessive humidity.

removing its top case.

receptacle may produce electrical shock and fire hazards.

cord directly to an appropriate receptacle with a functional ground.

Repackaging and Shipping

If you need to ship the instrument to BioTek for service or repair,



contact BioTek for a service authorization number, and be sure to
use the original packing materials. Other forms of commercially
available packaging are not recommended and can void the

warranty. If the original packing materials have been damaged or

lost, contact BioTek for replacement packing.

Warnings

Bright sunlight or strong incandescent light can reduce the linear performance range
of the instrument.

Measurement values may be affected by extraneous particles (such as dust) in the
microplate wells. A clean work area is necessary to ensure accurate readings.

When operated in a safe environment according to the instructions in this document,
there are no known hazards associated with the instrument. However, the operator
should be aware of certain situations that could result in serious injury; these may
vary depending on the instrument model. See

Hazards and Precautions.

Hazards

The following hazard warnings are provided to help avoid injury:

Warning! Internal Voltage. Always turn off the power switch and unplug

the power supply before cleaning the outer surface of the instrument or

Warning! Power Rating. The instrument’s power supply or power cord

must be connected to a power receptacle that provides voltage and current
within the specified rating for the system. Use of an incompatible power

Warning! Electrical Grounding. Never use a plug adapter to connect

primary power to the external power supply. Use of an adapter disconnects
the utility ground, creating a severe shock hazard. Always connect the power

BioTek Instruments, Inc.

Hazards | xi

specifications shall be used with the instrument.

people when lifting and carrying the instrument.

carrier path and cause the instrument to produce an error.

instrument if internal components have been exposed to fluid.

hazardous condition.

.

thoroughly analyzed by the operator.

chemically resistant rubber gloves and apron.

attempting replacement.

when the instrument is operating.

Warning! Service.

Only qualified technical personnel should perform

service procedures on internal components.

Warning! Accessories. Only accessories that meet the manufacturer’s

Warning! The instrument weighs approximately 38 pounds (17 kg). Use two

Warning! Lubricants. Do not apply lubricants to the microplate carrier or

carrier track. Lubricant on the carrier mechanism or components in the carrier
compartment will attract dust and other particles, which may obstruct the

Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage

into internal components creates a potential for shock hazard or instrument
damage. If a spill occurs while a program is running, abort the program and
turn the instrument off. Wipe up all spills immediately. Do not operate the

Warning! Unspecified Use. Failure to operate this equipment according to

the guidelines and safeguards specified in this manual could result in a

Warning! Software Quality Control. The operator must follow the

manufacturer’s assay package insert when modifying software parameters
and establishing reading, washing, or dispensing methods.

conduct quality control checks could result in erroneous test data

Failure to

Warning! Reader Data Reduction Protocol. No limits are applied to the

raw absorbance data. All information exported via computer control must be

Warning! Potential Biohazards. Some assays or specimens may pose a

biohazard. Adequate safety precautions should be taken as outlined in the
assay’s package insert. This hazard is noted by the symbol shown here.
Always wear safety glasses and appropriate protective equipment, such as

Warning! Hot Surface. The lamp assembly is hot when the instrument is

turned on. Turn off the reader and allow the lamp to cool down before

Warning! Pinch Hazard. Some areas of the dispense module can present

pinch hazards when the instrument is operating. The module is marked with
one of the symbols shown here. Keep hands/fingers clear of these areas

Synergy HTX Operator’s Manual

xii | Preface

and potentially impair instrument performance or cause damage to the instrument.

limits are broader.

and thoroughly wipe all surfaces.

Operate this power supply within the range of line voltages listed on it.

“on waste electrical and electronic equipment (WEEE),” or local ordinances.

the warranty.

shipment.

intended.

RF sources), because these may interfere with the proper operation.

Precautions

The following precautions are provided to help avoid damage to the instrument:

Caution: Service. The instrument should be serviced by BioTek authorized service

personnel. Only qualified technical personnel should perform troubleshooting and
service procedures on internal components.

Caution: Spare Parts. Only approved spare parts should be used for maintenance.

The use of unapproved spare parts and accessories may result in a loss of warranty

Caution: Environmental Conditions. Do not expose the instrument to

temperature extremes. For proper operation, ambient temperatures should remain
within the range listed in the
affected if temperatures fluctuate above or below this range. Storage temperature

Caution: Sodium Hypochlorite. Do not expose any part of the instrument to the

recommended diluted sodium hypochlorite solution (bleach) for more than 20
minutes. Prolonged contact may damage the instrument surfaces. Be certain to rinse

Specifications section. Performance may be adversely

Caution: Power Supply. Only use the power supply shipped with the instrument.

Caution: Disposal. Dispose of the instrument according to Directive 2002/96/EC,

Caution: Warranty. Failure to follow preventive maintenance protocols may void

Caution: Shipping Hardware. All shipping hardware must be removed before

operating the instrument and reinstalled before repackaging the instrument for

Caution: Electromagnetic Environment. Per IEC 61326-2-6 it is the user’s

responsibility to ensure that a compatible electromagnetic environment for this
instrument is provided and maintained in order that the device will perform as

Caution: Electromagnetic Compatibility. Do not use this device in close

proximity to sources of strong electromagnetic radiation (e.g., unshielded intentional

BioTek Instruments, Inc.

CE Mark

Based on the testing described below and information contained
herein, this instrument bears the CE mark

 See the Declaration of Conformity for more information.

Directive 2004/108/EC: Electromagnetic Compatibility

Emissions—Class A

The system has been type-tested by an independent, accredited testing laboratory
and found to meet the requirements of EN 61326-1: Class A for Radiated Emissions
and Line Conducted Emissions. Verification of compliance was conducted to the
limits and methods of EN 55011 – (CISPR 11) Class A. In a domestic environment it
may cause radio interference, in which case you may need to mitigate the
interference.

CE Mark | xiii

Immunity

The system has been type-tested by an independent, accredited testing laboratory
and found to meet the requirements of EN 61326-1 and EN 61326-2-6 for Immunity.
Verification of compliance was conducted to the limits and methods of the
following:

EN 61000-4-2, Electrostatic Discharge
EN 61000-4-3, Radiated EM Fields
EN 61000-4-4, Electrical Fast Transient/Burst
EN 61000-4-5, Surge Immunity
EN 61000-4-6, Conducted Disturbances from RFI
EN 61000-4-11, Voltage Dips, Short Interruptions and Variations

Directive 2006/95/EC Low Voltage (Safety)

The system has been type-tested by an independent testing laboratory and was found
to meet the requirements of this Directive. Verification of compliance was conducted to
the limits and methods of the following:

EN 61010-1. “Safety requirement for electrical equipment for measurement, control and
laboratory use. Part 1, General requirements.”

EN 61010-2-081, “Particular requirements for automatic and semi-automatic laboratory
equipment for analysis and other purposes.”

Synergy HTX Operator’s Manual

xiv | Preface

EN 61010-2-010, “Particular requirements for laboratory equipment for the heating of
materials.”

Directive 2002/96/EC: Waste Electrical and Electronic Equipment

Disposal Notice: Dispose of the instrument according to Directive 2002/96/EC, “on

waste electrical and electronic equipment (WEEE)” or local ordinances.

Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use)

• Product registration with competent authorities.

• Traceability to the U.S. National Institute of Standards and Technology (NIST).

• EN 61010-2-101, “Particular requirements for in vitro diagnostic (IVD) medical

equipment.”

Electromagnetic Interference and Susceptibility

USA FCC CLASS A

RADIO AND TELEVISION INTERFERENCE

Note:

This equipment has been tested and found to comply with the limits for a

Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are
designed to provide reasonable protection against harmful interference when the
equipment is operated in a commercial environment. Like all similar equipment,
this equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful
interference to radio communications. Operation of this equipment in a residential
area is likely to cause interference, in which case users will be required to correct
the interference at their own expense.

In order to maintain compliance with FCC regulations, shielded cables must be
used with this equipment. Operation with non-approved equipment or unshielded
cables is likely to result in interference to radio and television reception.

Canadian Department of Communications Class A

This digital apparatus does not exceed Class A limits for radio emissions from
digital apparatus set out in the Radio Interference Regulations of the Canadian
Department of Communications.

Le present appareil numerique n'emet pas de bruits radioelectriques depassant les
limites applicables aux appareils numerique de la Class A prescrites dans le
Reglement sur le brouillage radioelectrique edicte par le ministere des
Communications du Canada.

BioTek Instruments, Inc.

User Safety | xv

User Safety

This device has been type-tested by an independent laboratory and found to meet the
requirements of the following:

• Underwriters Laboratories UL 61010-1, “Safety requirements for electrical
equipment for measurement, control and laboratory use; Part 1: General
requirements.”

• Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety
requirements for electrical equipment for measurement, control and laboratory
use; Part 1: General requirements.”

• EN 61010 Standards, see

CE Mark starting on page xiii.

Synergy HTX Operator’s Manual

xvi | Preface

Safety Symbols

Some of these symbols appear on the instrument or accessories:

Alternating current
Courant alternatif
Wechselstrom
Corriente alterna
Corrente alternata

Both direct and alternating current
Courant continu et courant alternatif
Gleich - und Wechselstrom
Corriente continua y corriente alterna
Corrente continua e corrente alternata

Direct current
Courant continu
Gleichstrom
Corriente continua
Corrente continua

On (Supply)
Marche (alimentation)
Ein (Verbindung mit dem

Netz)
Conectado
Chiuso

Off (Supply)
Arrêt (alimentation)
Aus (Trennung vom Netz)
Desconectado
Aperto (sconnessione dalla

rete di alimentazione)

Warning, risk of electric shock
Attention, risque de choc

électrique
Gefährliche elektrische schlag
Precaución, riesgo de

sacudida eléctrica
Attenzione, rischio di scossa

elettrica

Earth ground terminal
Borne de terre
Erde (Betriebserde)
Borne de tierra
Terra (di funzionamento)

Protective conductor terminal
Borne de terre de protection
Schutzleiteranschluss
Borne de tierra de protección
Terra di protezione

Caution (refer to accompanying documents)
Attention (voir documents d’accompanement)
Achtung siehe Begleitpapiere
Atención (vease los documentos incluidos)
Attenzione, consultare la doc annessa

Warning, risk of crushing or pinching
Attention, risque d’écrasement et pincement
Warnen, Gefahr des Zerquetschens und

Klemmen
Precaución, riesgo del machacamiento y

sejeción
Attenzione, rischio di schiacciare ed

intrappolarsi

Warning, hot surface
Attention, surface chaude
Warnen, heiße Oberfläche
Precaución, superficie caliente
Attenzione, superficie calda

BioTek Instruments, Inc.

Warning, potential biohazards
Attention, risques biologiques potentiels
Warnung! Moegliche biologische Giftstoffe
Atención, riesgos biológicos
Attenzione, rischio biologico

Safety Symbols | xvii

In vitro diagnostic medical
device

Dispositif médical de
diagnostic in vitro

Medizinisches In-Vitro­Diagnostikum

Dispositivo médico de
diagnóstico in vitro

Dispositivo medico
diagnostico in vitro

Separate collection for electrical and
electronic equipment

Les équipements électriques et
électroniques font l’objet d’une collecte
sélective

Getrennte Sammlung von Elektro- und
Elektronikgeräten

Recogida selectiva de aparatos eléctricos y
electrónicos

Raccolta separata delle apparecchiature
elettriche ed elettroniche

Consult instructions for use
Consulter la notice d’emploi
Gebrauchsanweisung beachten
Consultar las instrucciones de

uso
Consultare le istruzioni per

uso

Synergy HTX Operator’s Manual

xviii | Preface

BioTek Instruments, Inc.

Chapter 1

Introduction

This chapter introduces the Synergy HTX, describes its key
features, lists its package contents, and provides contact
information for technical assistance.

Synergy HTX Multi-Mode Microplate Reader ............................... 2

Package Contents ................................................................... 3

Optional Accessories ............................................................... 4

Product Support & Service ....................................................... 5

Technical Assistance Center (TAC) ........................................ 5

Returning Instruments for Service/Repair .............................. 5

Applications Support .......................................................... 5

2 | Chapter 1: Introduction

Synergy HTX Multi-Mode Microplate Reader

The Synergy HTX is a single-channel microplate reader available with absorbance,
fluorescence, and luminescence detection. It is computer-controlled using BioTek’s Gen5
software for all operations including data reduction and analysis. Synergy HTX is robot
accessible and compatible with BioTek’s BioStack Microplate Stacker.

When making fluorescence determinations, the Synergy HTX uses a tungsten quartz
halogen lamp with interference filters for wavelength specificity in conjunction with a
photomultiplier (PMT) tube detector. The Synergy HTX has both top and bottom probes
for fluorescence measurements. The top probe can be adjusted vertically for the correct
reading height, via Gen5’s Read Height reading parameter (see

Started

Luminescence is measured by the low-noise PMT detector through an empty filter position
in the Emission filter wheel. A filter can also be left in place if light filtering is necessary.

Absorbance measurements are made by switching to a xenon flash lamp and a
monochromator for wavelength selection. The use of a xenon flash lamp allows for both
UV and visible light absorbance measurements. The monochromator provides wavelength
selection from 200 to 999 nm in 1-nm increments.

).

Chapter 3, Getting

The Synergy HTX has a 4-Zone temperature control from 4°C over ambient to 50°C,
controlled via a software-adjustable gradient. Internal plate shaking, with both linear and
orbital modes, is supported to ensure that reagents are properly mixed prior to reading.

Both Synergy HTX models support the reading of 6-, 12-, 24-, 48-, 96-, and 384-well
microplates with standard 128 x 86 mm geometry, as well as the BioTek Take3 and Take3
Trio Micro-Volume Plates. Absorbance mode reads plates up to 0.8" (20.3 mm) in height;
fluorescence mode reads plates up to 1.25" (31.75 mm). Polymerase Chain Reaction (PCR)
tubes up to 1.25" (31.75 mm) are also readable with the use of existing adapter plates.

For models with time-resolved fluorescence (TRF) capability, the TRF option allows
measurements by using the xenon flash light source in conjunction with the PMT
measurement detector. A special cartridge installed in the Excitation filter wheel location is
required.

Models with injectors support dual-reagent dispensing to 6-, 12-, 24-, 48-, 96-, and 384-well
microplates with standard 128 x 86 mm geometry. An external dispense module pumps
fluid from the supply bottles to the two injectors located inside the instrument. Both
injectors are positioned directly above the bottom probe, and fluid is injected into one well
at a time.

BioTek Instruments, Inc.

Package Contents | 3

Item

Part #

Package Contents

 Part numbers and package contents are subject to change. Contact

BioTek Customer Care with any questions.

Synergy HTX Operator’s Manual 1341000

Power supply 76061

Power cord set (specific to installation environment):

Europe (Schuko)
USA/International
United Kingdom
Australia/New Zealand

RS-232 serial cable 75034

USB cable

with USB Driver Software

Wrench 7772028

Fluorescence lamp assembly* (Note: The replacement lamp assembly is
PN 7080500)

Filter “plugs” (2) (also referred to as “dummy filters” or “blanks”)* 7082073

Plastic storage bag and fastener strips —

Time-Resolved Fluorescence cartridge assembly (“T” models only) 7090523

Models with injectors, an external dispense module (packed
separately), with the following accessories:

Outlet tubes (2, plus 2 spare) from dispense module to instrument
Inlet tubes (2) from supply bottles to syringe drives
250 µl syringes (2)
Syringe thumbscrews (2)
Priming plate
Injector tip priming trough
Dispense module communication cable
Dispense module front cover
Supply bottles (2, 30 mL)
Supply bottle holder assemblies (2)
Injector tip cleaning stylus and plastic storage bag

* If applicable to your reader model.

7090204

7080501

7082120
7082121
7083000

7132158
1342017

7082137
7122609
7090564
2872304

75010
75011
75012
75013

75108

19511

75107

Synergy HTX Operator’s Manual

4 | Chapter 1: Introduction

Item

Part #

Optional Accessories

 Accessory availability and part numbers are subject to change. Contact

BioTek Customer Care with questions or visit www.biotek.com and use
the Accessories search tool.

7-filter Absorbance Test Plate

Fluorescence Test Plate

Product Qualification (IQ-OQ-PQ) package

PCR Tube Adapter Plates

Terasaki Adapter Plate

Take3 Micro-Volume Plate

Take3 Trio Micro-Volume Plate

BioCell Quartz Vessel and Adapter Plate

Additional Fluorescence Filters; contact BioTek for part numbers and availability

The Synergy HTX is compatible with the BioStack Microplate Stacker. Contact BioTek or
visit our website to learn more.

7260522

7092092

1340508

6002072 and

6002076

7330531

TAKE3

Take3Trio

7272051/7270512

For Use with Liquid Tests (see Chapter 5) Part #

Absorbance Liquid Test Solutions:
BioTek Wetting Agent Solution
BioTek QC Check Solution #1
25 mL
125 mL

Dispense Module Liquid Test Solution:
BioTek Green Test Dye
BioTek Blue Test Dye
BioTek QC (Yellow) Test Dye

Individual Fluorescence Liquid Test Solutions:
Sodium Fluorescein Powder
Liquid Test Kit using Sodium Fluorescein
Liquid Test Kit using Methylumbelliferone (“MUB”)

7773002

7120779
7120782

7773003
7773001
7120782

98155
7160013
7160012

BioTek Instruments, Inc.

Product Support & Service

Technical Assistance Center (TAC)

If your instrument(s) or software fails to function properly, if you have questions about
how to use or maintain our products, or if you need to send an instrument to BioTek
for service or repair, please contact our Technical Assistance Center. BioTek’s “TAC” is
open from 8:30 AM to 5:30 PM (EST), Monday through Friday, excluding standard U.S.
holidays. You can send a fax or an e-mail any time. You can also request technical
assistance via our website:

Phone: [phone] or Fax: [phone] E-Mail: [e-mail]

[phone]

Please be prepared to provide the following information:

• Your name and company information, along with a daytime phone or fax
number, and/or an e-mail address

• The product name, model, and serial number

www.biotek.com.

Product Support & Service | 5

Web: www.biotek.com

• The onboard software part number and version (available through Gen5 at

System > Instrument Configuration > Get Basecode Information)

• Gen5 software version information (

Help > About Gen5)

• For troubleshooting assistance or instruments needing repair, the specific steps
that produce your problem and any error codes displayed in Gen5
(see also

Appendix C, Error Codes)

• A text file of the diagnostic history of the instrument (available via Gen5 by
selecting
file and clicking

System > Diagnostics > History, then selecting the appropriate

Export)

Returning Instruments for Service/Repair

If you need to return an instrument to BioTek for service or repair, please contact the
TAC for a service authorization number before shipping the instrument. Repackage the
instrument properly (see

Chapter 2, Installation), write the number on the shipping

box, and ship to BioTek.

Applications Support

BioTek’s fully equipped Application Laboratory provides our on-staff scientists with
the means to assist you with the integration of our instrumentation and software with
your unique scientific applications. If you are having difficulty with optimizing
fluorescence sensitivity or integrating a unique data reduction transformation, or you
are just looking for a recommendation on an appropriate fluorophore, contact us.

Phone: [phone] E-Mail: [e-mail]

Synergy HTX Operator’s Manual

6 | Chapter 1: Introduction

BioTek Instruments, Inc.

Chapter 2

Installation

This chapter includes instructions for unpacking and setting up
the Synergy HTX and, if applicable, the external dispense module.
Instructions are also included for repackaging the reader and
dispense module for shipment.

Product Registration ............................................................... 8

1: Unpack and Inspect the Reader ............................................ 8

2: Remove the Shipping Panel ............................................... 10

3: Remove the Microplate Carrier Shipping Screw ..................... 11

4: Install the Fluorescence Lamp Assembly .............................. 12

5: Select an Appropriate Location ........................................... 13

6: Connect the Power Supply ................................................. 14

7: Unpack and Inspect the Dispense Module ............................ 15

8: Install the Dispense Module ............................................... 18

9: Connect the Host Computer ............................................... 20

10: Install Gen5 Software ...................................................... 20

11: Turn on the Reader ......................................................... 20

12: Establish Communication ................................................. 21

13: Set the Dispenser Calibration Values ................................. 22

14: Run a System Test ......................................................... 23

15: Test the Injector System ................................................. 24

Operational/Performance Qualification ..................................... 26

Repackaging and Shipping Instructions ................................... 26

8 | Chapter 2: Installation

Product Registration

Please register your product with BioTek to ensure that you receive important information
and updates about the products you have purchased. Contact the Customer Resource
Center (CRC) at www.biotek.com or by calling 888-451-5171 or 802-655-4740.

1: Unpack and Inspect the Reader

Important! Save all packaging materials. If you need to ship the



reader to BioTek for repair or replacement, you must use the
original materials. Using other forms of commercially available
packaging, or failing to follow the repackaging instructions, may

void your warranty. Improper packaging that results in damage

to the instrument may lead to additional charges.

During the unpacking process, inspect the packaging, reader, and
accessories for shipping damage. If the reader is damaged, notify
the carrier and your BioTek representative. Keep the shipping
boxes and the packaging materials for the carrier’s inspection.
BioTek will arrange for repair or replacement of your reader
immediately.

1. Open the outer shipping box. Remove the foam blocks to access the inner box.

2. Carefully open the inner shipping box. Remove the accessories box and set it aside.
Remove the vertical supports.

 Warning! The instrument weighs approximately 38 pounds (17 kg).

Use two people when lifting and carrying the instrument.

3. The Synergy HTX is attached to a shipping panel that has two handles for lifting.
Locate and grasp the handles. Carefully lift the reader out of the box and place it on
a level surface. Remove the protective plastic bag.

4. Place all packing material back into the shipping box for reuse if the reader needs to
be shipped again.

 See Package Contents in Chapter 1 for assistance with identifying

the contents of the accessories box.

BioTek Instruments, Inc.