Biotek Synergy HT Operator's Manual
Operator’s Manual
Multi-Mode Microplate Reader
Synergy
™
HT
Synergy HT
Multi-Mode Microplate Reader
Operator’s Guide
October 2012
© 2012
Part Number 7091000
Revision L
BioTek® Instruments, Inc.
ii | Preface
Notices
BioTek® Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
All Rights Reserved
© 2012, BioTek® Instruments, Incorporated. No part of this publication may be
reproduced, transcribed, or transmitted in any form, or by any means electronic or
mechanical, including photocopying and recording, for any purpose other than the
purchaser’s use without written permission of BioTek Instruments, Inc.
Trademarks
BioTek® is a registered trademark, and Synergy™ HT, Gen5™, BioCell™, 4-Zone™
and BioStack™ are trademarks of BioTek Instruments, Inc.
Microsoft
Microsoft Corporation in the United States and/or other countries.
All other trademarks are the property of their respective holders.
®
, Windows®, and Excel® are either registered trademarks or trademarks of
Restrictions and Liabilities
Information in this document is subject to change and does not represent a
commitment by BioTek Instruments, Inc. Changes made to the information in this
document will be incorporated in new editions of the publication. No responsibility is
assumed by BioTek for the use or reliability of software or equipment that is not
supplied by BioTek or its affiliated dealers.
BioTek Instruments, Inc.
Contents
Contact Information ........................................................... v
Document Conventions .......................................................vi
Revision History ............................................................... vii
Intended Use Statement .....................................................xi
Quality Control ..................................................................xi
Warranty and Product Registration .......................................xi
Repackaging and Shipping ................................................. xii
Warnings ......................................................................... xii
Hazards ........................................................................... xii
Precautions ..................................................................... xiii
CE Mark .......................................................................... xv
Electromagnetic Interference and Susceptibility ................... xvi
User Safety .................................................................... xvii
Safety Symbols ............................................................. xviii
Chapter 1: Introduction ........................................................ 1
Synergy HT Multi-Mode Microplate Reader ............................. 2
Features ........................................................................... 3
Package Contents .............................................................. 4
Optional Accessories .......................................................... 5
Product Support & Service .................................................. 6
Chapter 2: Installation ......................................................... 7
Product Registration ........................................................... 8
1: Unpack and Inspect the Reader ....................................... 8
Contents | iii
Customer Service and Sales ............................................ v
Service/TAC ................................................................. v
European Coordination Center/Authorized European
Representative .............................................................. v
Directive 2004/108/EC: Electromagnetic Compatibility ...... xv
Directive 2006/95/EC Low Voltage (Safety) ...................... xv
Directive 2002/96/EC: Waste Electrical and Electronic
Equipment ................................................................. xvi
Directive 98/79/EC: In Vitro Diagnostics (if labeled for this
use) .......................................................................... xvi
USA FCC CLASS A ....................................................... xvi
Canadian Department of Communications Class A ............ xvi
Technical Assistance Center (TAC) ................................... 6
Returning Instruments for Service/Repair ......................... 6
Contacting BioTek for Applications Support ....................... 6
Synergy HT Operator’s Manual
iv | Preface
Chapter 3: Getting Started ................................................. 33
Chapter 4: Instrument Qualification ................................... 53
2: Remove the Shipping Panel ........................................... 10
3: Remove the Microplate Carrier Shipping Screw ................. 12
4: Install the Fluorescence Lamp Assembly .......................... 13
5: Select an Appropriate Location ....................................... 14
6: Connect the Power Supply............................................. 14
7: Unpack and Inspect the Dispense Module ........................ 15
8: Install the Dispense Module ........................................... 19
9: Connect the Host Computer .......................................... 23
10: Install Gen5 Software ................................................. 23
11: Turn on the Reader .................................................... 23
12: Establish Communication ............................................ 24
13: Run a System Test ..................................................... 25
14: Test the Injector System ............................................. 26
Operational/Performance Qualification ................................ 28
Repackaging and Shipping Instructions ............................... 28
Key Components ............................................................. 34
Power Switch, Carrier Eject Button, Microplate Carrier ...... 34
Lamp Assembly and Filter Wheel Access ......................... 35
Excitation and Emission Filter Wheels ............................. 36
Installing the Time-Resolved Fluorescence Cartridge ........ 39
Configuring the System for Luminescence Measurements .. 40
The External Dispense Module ....................................... 41
Gen5 Software ................................................................ 44
Viewing/Updating the Filter and Wavelengths Tables ........ 44
Creating Protocols and Experiments ............................... 45
Controlling the Dispense Module .................................... 49
Recommendations for Optimum Performance ....................... 51
Overview ........................................................................ 54
IQ/OQ/PQ ....................................................................... 54
Recommended Qualification Schedule ................................. 56
System Test .................................................................... 57
Absorbance Plate Test ...................................................... 62
Test Plate Certificates .................................................. 62
Define Absorbance Test Plate Parameters ....................... 62
Run the Absorbance Plate Test ...................................... 63
Results and Troubleshooting Tips ................................... 66
Absorbance Liquid Tests ................................................... 68
rbance Liquid Test 1 .............................................. 69
o
Abs
Absorbance Liquid Test 2 .............................................. 71
Absorbance Liquid Test 3 (optional) ............................... 74
BioTek Instruments, Inc.
Contents | v
Fluorescence Tests ........................................................... 76
Required Materials ....................................................... 77
Test Solutions ............................................................. 78
Procedure .................................................................. 79
Results Analysis .......................................................... 79
Troubleshooting .......................................................... 80
Pipette Map ................................................................ 81
Gen5 Protocol Reading Parameters ................................ 82
Fluorescence Tests Using Methylumbelliferone ................. 83
Dispense Module Tests ..................................................... 86
Required Materials ....................................................... 87
Alternate Test Solutions ............................................... 88
Test Setup ................................................................. 89
Test Procedure ............................................................ 89
Results Analysis .......................................................... 91
Creating the Test Protocols ........................................... 92
Chapter 5: Preventive Maintenance .................................... 93
Recommended Maintenance Schedule ................................. 94
Overview ................................................................... 94
Daily Cleaning for the Dispense Module .......................... 94
Recommended Maintenance Schedule ............................ 95
Warnings & Precautions .................................................... 96
Cleaning Exposed Surfaces ................................................ 97
Inspect/Clean Excitation and Emission Filters ....................... 98
Flush/Purge the Fluid Path ................................................ 99
Running a Dispense Protocol (Optional) ............................. 100
Empty/Clean the Tip Priming Trough ................................. 101
Clean the Priming Plate .................................................... 101
Clean the Internal Components ......................................... 102
Required Materials ...................................................... 103
Removing the Reader’s Shroud ..................................... 104
Removing the Internal Tubes and Injector Heads ............ 106
Cleaning the Internal Tubes and Injector Heads .............. 109
Cleaning the Optical Probes ......................................... 110
Cleaning the Reader’s Internal Surface .......................... 118
Reassembling the Components ..................................... 11
Perf
ormance Check ..................................................... 120
9
Appendix A: Decontamination .......................................... 123
Purpose ......................................................................... 124
Required Materials .......................................................... 125
Procedure for Models without Injectors .............................. 126
Routine Procedure for Models with Injectors ....................... 127
Synergy HT Operator’s Manual
vi | Preface
Appendix B: Computer Control ......................................... 133
Appendix C: Error Codes ................................................... 135
Appendix D: Specifications ............................................... 179
Appendix E: Instrument Dimensions for Robotic Interface187
Clean Exposed Surfaces .............................................. 127
Decontaminate the Fluid Lines ...................................... 128
Rinse the Fluid Lines ................................................... 129
Clean the Internal Tubing and Injector Heads ................. 129
Clean the Tip Priming Trough and Priming Plate .............. 130
Alternate Procedure for Models with Injectors ..................... 131
Error Codes ................................................................... 136
Fatal Errors ............................................................... 137
Non-Fatal Errors ......................................................... 138
Status String Format ....................................................... 173
Technical Specifications ................................................... 180
General Specifications ................................................. 180
Absorbance Specifications ........................................... 181
Fluorescence Specifications .......................................... 183
Models with Injectors .................................................. 185
Instrument Dimensions for Robotic Interface ...................... 188
BioTek Instruments, Inc.
Contact Information
BioTek® Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
Customer Service and Sales
Internet: www.biotek.com
Phone: 888-451-5171 (toll-free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: 802-655-7941
E-Mail: customercare@biotek.com
Service/TAC
Contact Information | v
Phone: 800-242-4685 (toll-free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: 802-654-0638
E-Mail: tac@biotek.com
European Coordination Center/Authorized European
Representative
BioTek® Instruments GmbH
Kocherwaldstrasse 34
D-74177 Bad Friedrichshall
Germany
Internet: www.biotek.de
Phone: +49 (0) 7136 9680
Fax: +49 (0) 7136 968 111
E-Mail: info@biotek.de
Synergy HT Operator’s Manual
vi | Preface
Document Conventions
This icon calls attention to important safety notes.
Warning!
Caution
Note:
italic
A Warning indicates the potential for bodily harm and
tells you how to avoid the problem.
A Caution indicates potential damage to the instrument
and tells you how to avoid the problem.
Bold text is primarily used for emphasis.
Topics that apply only to specific Synergy HT models are
preceded by a notice in italics, for example: Applies only to
Synergy HT models with injectors.
This icon calls attention to important information.
BioTek Instruments, Inc.
Revision History | vii
Revision History
Rev Date Changes
A 4/2002 First issue
B 8/2002 Added Time-Resolved Mode
C 8/2003 Added Dual Fluid Dispense Feature
Ch. 1, Introduction: Updated specifications, accessories, and technical support.
Ch. 2, Instrument Description: Updated component descriptions and added
drawings.
Ch. 3, Installation: Revised Dispenser Module setup instructions and KC4 launch
procedure. Revised unpacking/repackaging instructions.
Added new Chapter 4, “Getting Started With KC4.”
Ch. 5, Performance Verification/Qualification Tests (formerly Chapter 4): Revised
test procedures.
Reformatted Appx A, Decontamination, and Appendix B, Computer Control.
Updated Appx C, Error Codes. Renamed Appx D, Microplate Location Dimensions
to “Instrument Dimensions.”
D 12/2003 Preface: Updated safety symbols and text (p. ix and x). Updated Intended Use
Statement (p. xi). Revised Warranty (p. xii).
Chapter 1: Modified Introduction (p. 1-3). Updated list of optional accessories (p.
1-4). Revised absorbance reading speed information (p. 1-5). Clarified
fluorescence specifications (p. 1-8 and 1-9). Added specifications for injector
model (p. 1-10).
Chapter 2: Clarified description of external and internal components of the
injector model and updated drawings. Moved procedure for replacing the lamp
assembly to Ch. 6.
Chapter 5: Added note regarding the availability of the Installation-OperationalPerformance (IQ-OQ-PQ) package (PN 7090521) (p. 5-2). Updated liquid test
procedures.
Added new Chapter 6, Maintenance and Troubleshooting, which includes: Sample
reproducible page from maintenance logbook; Procedures for maintenance and
routine cleaning; Instructions for changing injector positions.
Updated decontamination procedure (Appendix A). Modified Appendix B,
Computer Control. Corrected Appendix C, Error Codes. Changed “Dispenser
Module” to “Dispense Module” throughout.
E 2/2004 Chapter 1, Introduction: Removed reference to “NB” version (p. 1-3). Added
specifications to reflect the use of an additional PMT type (R4220PHA) to
Hardware Features (p. 1-3). Moved filter plug (7082073) from Optional
Accessories list to Package Contents (p. 1-4). Added required specifications for
microplates used in “Luminescence” mode
(p. 1-5). Added “6- to 96-well plates” and “fluorescence and luminescence read
modes” to Injector Model features (p. 1-10). Removed “option” from reference to
Incubation specifications (p. 1-11).
Chapter 3, Installation: Removed references to “NB” version from description of
reader model options (Setting Communication Parameters in KC4, p. 3-12).
Chapter 5, Performance Verification/Qualification Tests: Updated Figure 5-1,
Sample System Test (p. 5-6 and 5-7).
Updated Appendix C, Error Codes.
Synergy HT Operator’s Manual
viii | Preface
Rev Date Changes
F 7/2004 General: Edited and reformatted text according to new template. Added
photographs for clarification as needed.
Chapter 1: Updated Optional Accessories list.
Chapter 2: Updated text and graphics to describe/illustrate: Removal of the front
injector. Redesign of the priming plate, to limit splatter. Improved system design
to reduce need for periodic maintenance. Elimination of the right-front tip priming
trough, and redesign of the left-rear trough. Improved bottle holder setup.
Chapter 3: Updated diagram showing removal of Dispense Module from inner
shipping box. Updated procedure for setting up Dispense Module on the Injector
Model.
Chapter 4: Removed instructions for setting injector position.
Chapter 6: Added preventive maintenance procedure for periodic cleaning of the
top/bottom fluorescence optical probes and the absorbance read channel optical
path.
Appendix A: Updated drawing of tip priming trough and priming plate.
G 8/2005 Updated Warranty information.
Moved “Specifications” from Chapter 1 to Appendix D. Corrected operating
temperature (18-40°C) and injector accuracy (±1 µl at 5-50 µl) to match
published specifications.
Removed Chapter 2, “Instrument Description” and distributed the information and
photos among the remaining chapters.
Reorganized the flow of the “Installation” chapter to better represent actual
practice. Added test to verify the injector system setup.
Changed the former “Getting Started with KC4” chapter to a broader “Getting
Started” chapter that includes information on the key instrument components.
Added new topic for configuring the system for luminescence reads.
Renamed the “Performance Verification/Qualification Tests” chapter to
“Instrument Qualification.” Replaced former Dispense Precision & Accuracy Tests
with new tests that use a single green dye solution and a single microplate.
Restructured the “Preventive Maintenance” chapter to better represent actual
practice. Added a recommended maintenance table for models without injectors.
Added new photos to help with identification of the various components.
Updated the “Error Codes” appendix with recent information.
Additional minor corrections and improvements throughout.
H 5/2006 Redesigned the front cover. Removed unnecessary Warranty information; a
Warranty card ships with every instrument. Added warning to shut down
instrument and wait for the fluorescence lamp to cool down before replacing it.
Added the PN for a replacement fluorescence lamp (7080500).
For models with injectors: Simplified the installation and setup steps for the
Dispense Module. Added recommendation to set a tip prime volume equal to the
per-well dispense volume for volumes < 20 µl.
Updated Absorbance Plate Test instructions related to Peak Wavelength, to
support the modified 7-filter test plate.
Simplified the process for creating Titration Dyes for the Fluorescence (SF)
Sensitivity Test. Added information to the pass/fail criteria table for the (SF)
Sensitivity Test.
Clarified that for models without injectors, the reader’s internal chamber and
optical probes are not user-accessible for cleaning.
Updated the “Error Codes” appendix with recent information.
Additional minor cosmetic changes throughout.
Added/modified instructions throughout to support Gen5™, including:
Chapter 2, Installation - Added instructions for installing software, establishing
BioTek Instruments, Inc.
Revision History | ix
Rev Date Changes
(H) communication with the reader, installing/testing dispense module components.
Chapter 3, Getting Started - Added introductory information for new Gen5 users.
Chapter 4, Instrument Qualification - Added instructions for performing the
System Test, Absorbance Plate Test, and Dispense Accuracy & Precision Test.
Chapter 5, Preventive Maintenance - Added instructions for creating the optional
Dispense protocol in Gen5.
I 11/2008 Throughout: Changed product description from “Multi-Detection” to “Multi-Mode”.
Changed “Bio-Stack” to “BioStack.”
Preface: Corrected Service/TAC fax number. Updated the Intended Use section
with respect to IVD labeling. Added cautions for Electromagnetic Environment and
Compatibility. Updated Directives. Added ‘Pinch hazard’ to Hazards and
Precautions. Added ‘Consult Instructions for use’ and ‘IVD’ to Safety Symbols.
Chapter 1, Introduction: In the product introduction section, added note that
Synergy HT basecode software version 2.24 or greater is required for use with
Gen5™. Under ‘Package Contents’ added notice that part numbers are subject to
change over time, and updated part numbers for the priming plate and tip priming
trough.
Chapter 2, Installation: Added section “Product Registration.”
Chapter 4, Instrument Qualification: Modified sample System Test Report and
Absorbance Test Plate Results to reflect more current date and minimum
basecode for Synergy HT to work with Gen5. Absorbance Liquid Tests section:
Liquid Test 3, removed instructions for creating the rarely used Buffer Solution A.
Fluorescence Liquid Tests section: Added option to use Sodium Borate instead of
PBS with sodium fluorescein.
Reconfigured the SF test solutions, dilutions, and pipette maps for efficiency and
consistency with other BioTek products. Added option to use Methylumbelliferone
to test the top optics. Dispense Module Tests section: Corrected the formula for
Accuracy % Error.
Chapter 5, Preventive Maintenance: Removed unnecessary “Clean Supply Bottle”
section. Modified the Running a Dispense Protocol procedure to include running
the experiment and inspecting the plate. Added a missing word to the Cleaning
the Optical Probes section.
Appendix C, Error Codes: Corrected the range of error codes under Home Sensor
Initial Find Errors. Removed some text from the Status String Format section that
was misleading.
J 7/2011 General: Removed references to outdated software KC4. Updated instructions for
Gen5 2.x.
Preface: Updated Trademarks, the Intended Use Statement, Hazards, CE Mark,
and sequence.
Chapter 1: Introduction: Added info on BioTek SF and MUB liquid test kits to
optional accessories. Updated Power Supply part number.
Chapter 3: Getting Started: Added a recommendation regarding filter positions in
the wheels (p. 36).
Chapter 4: Instrument Qualification: Under test solutions, corrected the stock
solution unit of measure (nM to mM). Added references to BioTek SF and MUB
liquid test kits.
Appendix C: Error Codes: Added new Probable Cause to code 0508.
Synergy HT Operator’s Manual
x | Preface
Rev Date Changes
K 7/2012 General: Updated instructions to include terminology changes in Gen5 v2.x;
added support for the Take3 and Take3 Trio plates; added text to indicate
sections that apply only to certain models (i.e., models with a dispenser)
Preface: Updated date and revision on title page and in Notices; in “Hazards,”
added “Service” and “Accessories” warnings; in “Precautions,” added “Spare
Parts” caution; updated CE Mark text.
Chapter 1, Introduction: Updated the format of “Package Contents” and “Optional
Contents”.
Chapter 2, Installation: Added “Try a different COM Port” to “12: Establish
Communication.”
Chapter 4, Qualification: Updated the Absorbance Plate Test section
L 10/2012 Preface: Added a warning to use two people to lift/carry the instrument. Updated
the dispense module pinch hazard warning text and symbols. Updated the CE
Mark information to include EN 61010-2-081 and EN 61010-2-010.
Chapter 2, Installation: Added a warning to use two people to lift/carry the
instrument.
BioTek Instruments, Inc.
Intended Use Statement
• The Synergy HT is a single-channel absorbance, fluorescence, and luminescence
microplate reader that uses a dual-optics design to perform measurements of
samples in a microplate format. The performance characteristics of the data
reduction software have not been established with any laboratory diagnostic assay.
The user must evaluate this instrument and Gen5 software in conjunction with
their specific assay(s). This evaluation must include the confirmation that
performance characteristics for the specific assay(s) are met.
• If the instrument has an “IVD” label, it may be used for clinical and non-clinical
purposes, including research and development. If there is no such label, the
instrument may be used only for research and development or other non-clinical
purposes.
Quality Control
Intended Use Statement | xi
It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for the test
to be conducted. Failure to conduct Quality Control checks could result in erroneous test
data.
Warranty and Product Registration
Take a moment to review the Warranty information that shipped with your product.
Please also register your product with BioTek to ensure that you receive important
information and updates about the product(s) you have purchased. You can register online
through the Customer Resource Center at www.biotek.com or by calling 888/451-5171 or
802/655-4740.
Synergy HT Operator’s Manual
xii | Preface
Repackaging and Shipping
If you need to ship the instrument to BioTek for service or repair,
contact BioTek for a Return Materials Authorization (RMA)
number, and be sure to use the original packing materials. Other
forms of commercially available packaging are not recommended
and can void the warranty. If the original packing materials have
been damaged or lost, contact BioTek for replacement packing.
Warnings
Operate the instrument on a level, stable surface away from excessive humidity.
Bright sunlight or strong incandescent light can reduce the linear performance range
of the instrument.
Measurement values may be affected by extraneous particles (such as dust) in the
microplate wells. A clean work area is necessary to ensure accurate readings.
When operated in a safe environment according to the instructions in this document,
there are no known hazards associated with the instrument. However, the operator
should be aware of certain situations that could result in serious injury; these may
vary depending on the instrument model. See
Hazards and Precautions.
Hazards
The following hazard warnings are provided to help avoid injury:
Warning! Internal Voltage.
power supply before cleaning the outer surface of the instrument or removing its top
case.
Warning! Power Rating.
connected to a power receptacle that provides voltage and current within the
specified rating for the system. Use of an incompatible power receptacle may
produce electrical shock and fire hazards.
Always turn off the power switch and unplug the
The instrument’s power supply or power cord must be
BioTek Instruments, Inc.
Warning! Electrical Grounding.
power to the external power supply. Use of an adapter disconnects the utility
ground, creating a severe shock hazard. Always connect the power cord directly to
an appropriate receptacle with a functional ground.
Never use a plug adapter to connect primary
Hazards | xiii
Warning! Service.
Only qualified technical personnel should perform service
procedures on internal components.
Warning! Accessories. Only accessories that meet the manufacturer’s
specifications shall be used with the instrument.
Warning!
when lifting and carrying the instrument.
Warning! Lubricants.
The instrument weighs approximately 38 pounds (17 kg). Use two people
Do not apply lubricants to the microplate carrier or carrier
track. Lubricant on the carrier mechanism or components in the carrier compartment
will attract dust and other particles, which may obstruct the carrier path and cause
the instrument to produce an error.
Warning! Liquids.
Avoid spilling liquids on the instrument; fluid seepage into
internal components creates a potential for shock hazard or instrument damage. If a
spill occurs while a program is running, abort the program and turn the instrument
off. Wipe up all spills immediately. Do not operate the instrument if internal
components have been exposed to fluid.
Warning! Unspecified Use.
Failure to operate this equipment according to the
guidelines and safeguards specified in this manual could result in a hazardous
condition.
Warning! Software Quality Control.
The operator must follow the
manufacturer’s assay package insert when modifying software parameters and
establishing reading, washing, or dispensing methods.
control checks could result in erroneous test data
Failure to conduct quality
.
Warning! Reader Data Reduction Protocol.
No limits are applied to the raw
absorbance data. All information exported via computer control must be thoroughly
analyzed by the operator.
Warning! Potential Biohazards.
Some assays or specimens may pose a
biohazard. Adequate safety precautions should be taken as outlined in the assay’s
package insert. This hazard is noted by the symbol shown here. Always wear safety
glasses and appropriate protective equipment, such as chemically resistant rubber
gloves and apron.
Warning! Hot Surface.
The lamp assembly is hot when the instrument is turned
on. Turn off the reader and allow the lamp to cool down before attempting
replacement.
Warning! Pinch Hazard. Some areas of the dispense module can present
pinch hazards when the instrument is operating. The module is marked with
one of the symbols shown here. Keep hands/fingers clear of these areas
when the instrument is operating.
Synergy HT Operator’s Manual
xiv | Preface
Precautions
The following precautions are provided to help avoid damage to the instrument:
Caution: Service.
The instrument should be serviced by BioTek authorized service
personnel. Only qualified technical personnel should perform troubleshooting and
service procedures on internal components.
Caution: Spare Parts.
Only approved spare parts should be used for maintenance.
The use of unapproved spare parts and accessories may result in a loss of warranty
and potentially impair instrument performance or cause damage to the instrument.
Caution: Environmental Conditions.
Do not expose the instrument to
temperature extremes. For proper operation, ambient temperatures should remain
within the range listed in the
Specifications section. Performance may be adversely
affected if temperatures fluctuate above or below this range. Storage temperature
limits are broader.
Caution: Sodium Hypochlorite.
Do not expose any part of the instrument to the
recommended diluted sodium hypochlorite solution (bleach) for more than 20
minutes. Prolonged contact may damage the instrument surfaces. Be certain to rinse
and thoroughly wipe all surfaces.
Caution: Power Supply.
Only use the power supply shipped with the instrument.
Operate this power supply within the range of line voltages listed on it.
Caution: Disposal.
This instrument contains printed circuit boards and wiring with
lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste
electrical and electronic equipment (WEEE),” or local ordinances.
Caution: Warranty.
the warranty
Caution: Shipping Hardware.
.
Failure to follow preventive maintenance protocols may void
All shipping hardware must be removed before
operating the instrument and reinstalled before repackaging the instrument for
shipment.
Caution: Electromagnetic Environment.
Per IEC 61326-2-6 it is the user’s
responsibility to ensure that a compatible electromagnetic environment for this
instrument is provided and maintained in order that the device will perform as
intended.
Caution: Electromagnetic Compatibility.
Do not use this device in close
proximity to sources of strong electromagnetic radiation (e.g., unshielded intentional
RF sources), because these may interfere with the proper operation.
BioTek Instruments, Inc.
CE Mark
Based on the testing described below and information contained
herein, this instrument bears the CE mark
See the Declaration of Conformity for more information.
Directive 2004/108/EC: Electromagnetic Compatibility
Emissions—Class A
The system has been type-tested by an independent, accredited testing laboratory
and found to meet the requirements of EN 61326-1: Class A for Radiated Emissions
and Line Conducted Emissions. Verification of compliance was conducted to the
limits and methods of EN 55011 – (CISPR 11) Class A. In a domestic environment it
may cause radio interference, in which case you may need to mitigate the
interference.
CE Mark | xv
Immunity
The system has been type-tested by an independent, accredited testing laboratory
and found to meet the requirements of EN 61326-1 and EN 61326-2-6 for Immunity.
Verification of compliance was conducted to the limits and methods of the
following:
EN 61000-4-2, Electrostatic Discharge
EN 61000-4-3, Radiated EM Fields
EN 61000-4-4, Electrical Fast Transient/Burst
EN 61000-4-5, Surge Immunity
EN 61000-4-6, Conducted Disturbances from RFI
EN 61000-4-11, Voltage Dips, Short Interruptions and Variations
Directive 2006/95/EC Low Voltage (Safety)
The system has been type-tested by an independent testing laboratory and was found
to meet the requirements of this Directive. Verification of compliance was conducted to
the limits and methods of the following:
EN 61010-1. “Safety requirement for electrical equipment for measurement, control and
laboratory use. Part 1, General requirements.”
EN 61010-2-081, “Particular requirements for automatic and semi-automatic laboratory
equipment for analysis and other purposes.”
Synergy HT Operator’s Manual
xvi | Preface
EN 61010-2-010, “Particular requirements for laboratory equipment for the heating of
materials.”
Directive 2002/96/EC: Waste Electrical and Electronic Equipment
Disposal Notice: This instrument contains printed circuit boards and wiring with
lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste
electrical and electronic equipment (WEEE)” or local ordinances.
Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use)
• Product registration with competent authorities.
• Traceability to the U.S. National Institute of Standards and Technology (NIST).
• EN 61010-2-101, “Particular requirements for in vitro diagnostic (IVD) medical
equipment.”
Electromagnetic Interference and Susceptibility
USA FCC CLASS A
RADIO AND TELEVISION INTERFERENCE
Note:
This equipment has been tested and found to comply with the limits for a
Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are
designed to provide reasonable protection against harmful interference when the
equipment is operated in a commercial environment. Like all similar equipment,
this equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful
interference to radio communications. Operation of this equipment in a residential
area is likely to cause interference, in which case users will be required to correct
the interference at their own expense.
In order to maintain compliance with FCC regulations, shielded cables must be
used with this equipment. Operation with non-approved equipment or unshielded
cables is likely to result in interference to radio and television reception.
Canadian Department of Communications Class A
This digital apparatus does not exceed Class A limits for radio emissions from
digital apparatus set out in the Radio Interference Regulations of the Canadian
Department of Communications.
Le present appareil numerique n'emet pas de bruits radioelectriques depassant les
limites applicables aux appareils numerique de la Class A prescrites dans le
Reglement sur le brouillage radioelectrique edicte par le ministere des
Communications du Canada.
BioTek Instruments, Inc.
User Safety | xvii
User Safety
This device has been type-tested by an independent laboratory and found to meet the
requirements of the following:
• Underwriters Laboratories UL 61010-1, “Safety requirements for electrical
equipment for measurement, control and laboratory use; Part 1: General
requirements.”
• Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety
requirements for electrical equipment for measurement, control and laboratory
use; Part 1: General requirements.”
• EN 61010 Standards, see
CE Mark starting on page xv.
Synergy HT Operator’s Manual
xviii | Preface
Safety Symbols
Some of these symbols appear on the instrument or accessories:
Alternating current
Courant alternatif
Wechselstrom
Corriente alterna
Corrente alternata
Both direct and alternating current
Courant continu et courant alternatif
Gleich - und Wechselstrom
Corriente continua y corriente alterna
Corrente continua e corrente alternata
Direct current
Courant continu
Gleichstrom
Corriente continua
Corrente continua
On (Supply)
Marche (alimentation)
Ein (Verbindung mit dem
Netz)
Conectado
Chiuso
Off (Supply)
Arrêt (alimentation)
Aus (Trennung vom Netz)
Desconectado
Aperto (sconnessione dalla
rete di alimentazione)
Warning, risk of electric shock
Attention, risque de choc
électrique
Gefährliche elektrische schlag
Precaución, riesgo de
sacudida eléctrica
Attenzione, rischio di scossa
elettrica
Earth ground terminal
Borne de terre
Erde (Betriebserde)
Borne de tierra
Terra (di funzionamento)
Protective conductor terminal
Borne de terre de protection
Schutzleiteranschluss
Borne de tierra de protección
Terra di protezione
Caution (refer to accompanying documents)
Attention (voir documents d’accompanement)
Achtung siehe Begleitpapiere
Atención (vease los documentos incluidos)
Attenzione, consultare la doc annessa
Warning, risk of crushing or pinching
Attention, risque d’écrasement et pincement
Warnen, Gefahr des Zerquetschens und
Klemmen
Precaución, riesgo del machacamiento y
sejeción
Attenzione, rischio di schiacciare ed
intrappolarsi
Warning, hot surface
Attention, surface chaude
Warnen, heiße Oberfläche
Precaución, superficie caliente
Attenzione, superficie calda
BioTek Instruments, Inc.
Warning, potential biohazards
Attention, risques biologiques potentiels
Warnung! Moegliche biologische Giftstoffe
Atención, riesgos biológicos
Attenzione, rischio biologico
Safety Symbols | xix
In vitro diagnostic medical
device
Dispositif médical de
diagnostic in vitro
Medizinisches In-VitroDiagnostikum
Dispositivo médico de
diagnóstico in vitro
Dispositivo medico
diagnostico in vitro
Separate collection for electrical and
electronic equipment
Les équipements électriques et
électroniques font l’objet d’une collecte
sélective
Getrennte Sammlung von Elektro- und
Elektronikgeräten
Recogida selectiva de aparatos eléctricos y
electrónicos
Raccolta separata delle apparecchiature
elettriche ed elettroniche
Consult instructions for use
Consulter la notice d’emploi
Gebrauchsanweisung beachten
Consultar las instrucciones de
uso
Consultare le istruzioni per
uso
Synergy HT Operator’s Manual
xx | Preface
BioTek Instruments, Inc.
Chapter 1
Introduction
This chapter introduces the Synergy HT, describes its key features,
lists its package contents, and provides contact information for
technical assistance.
Synergy HT Multi-Mode Microplate Reader ................................. 2
Features ............................................................................... 3
Package Contents ................................................................... 4
Optional Accessories ............................................................... 5
Product Support & Service ....................................................... 6
Technical Assistance Center (TAC) ........................................ 6
Returning Instruments for Service/Repair .............................. 6
Contacting BioTek for Applications Support ............................ 6
2 | Chapter 1: Introduction
Synergy HT Multi-Mode Microplate Reader
The Synergy HT is a single-channel absorbance, fluorescence, and luminescence
microplate reader. It is computer-controlled using BioTek’s Gen5 software for all
operations including data reduction and analysis.
version
2.24 or greater is required for use with Gen5. The Synergy HT is robot
accessible and compatible with BioTek’s BioStack Microplate Stacker.
Note: Synergy HT basecode software
When making
fluorescence determinations, the Synergy HT uses a tungsten quartz
halogen lamp with interference filters for wavelength specificity in conjunction with a
photomultiplier (PMT) tube detector. The Synergy HT has both top and bottom probes for
fluorescence measurements. The top probe can be adjusted vertically for the correct
reading height, via Gen5’s Read Height reading parameter (see
Started
Luminescence is measured by the low-noise PMT detector through an empty filter
).
Chapter 3, Getting
position in the Emission filter wheel. A filter can also be left in place if light filtering is
necessary.
Absorbance measurements are made by switching to a xenon flash lamp and a
monochromator for wavelength selection. The use of a xenon flash lamp allows for both
UV and visible light absorbance measurements. The monochromator provides wavelength
selection from 200 to 999 nm in 1-nm increments.
The Synergy HT has a 4-Zone
temperature control from 4°C (39.2°F) over ambient to
50°C (122°F) that ensures superior temperature uniformity necessary for kinetic assays.
Internal plate shaking is also supported.
All Synergy HT models support the reading of 6-, 12-, 24-, 48-, 96-, and 384-well
microplates with standard 128 x 86 mm geometry, as well as the BioTek Take3 and Take3
Trio Micro-Volume Plates. Absorbance mode reads plates up to 0.8” (20.3 mm) in height;
fluorescence mode reads plates up to 1.25” (31.75 mm). Polymerase Chain Reaction (PCR)
tubes up to 1.25” (31.75 mm) are also readable with the use of existing adapter plates.
The Time-Resolved (TR) option allows
time-resolved fluorescence measurements by
using the xenon flash light source in conjunction with the PMT measurement detector. A
special cartridge installed in the Excitation filter wheel location is required.
Models with
microplates with standard 128 x 86 mm geometry. An
injectors support dual-reagent dispensing to 6-, 12-, 24-, 48-, and 96-well
external dispense module
pumps fluid from the supply bottles to the two injectors located inside the instrument.
Both injectors are positioned directly above the bottom probe, and fluid is injected into one
well at a time.
BioTek Instruments, Inc.
Features | 3
Features
• Operated using BioTek’s Gen5 Data Analysis Software
• Dual-optics design, with separate fluorescence and absorbance channels
• 3 mm top and 5 mm bottom fluorescence probes (standard configuration)
• Optional 1.5 mm top/bottom, 3 mm bottom fluorescence probes (custom
configurations)
• Optional Time-Resolved Fluorescence (TRF) capability (“T” models, e.g., SIAFRT)
• Fluorescence λ ranges:
Standard low-noise PMT
Optional red-extended PMT
• Absorbance λ range of 200 to 999 nm
¾ Excitation: 300 to 650 nm (200 to 700 nm with “T” models)
¾ Emission: 300 to 700 nm
¾ Excitation: 300 to 650 nm (200 to 800 nm with “T” models)
¾ Emission: 300 to 800 nm
• Absorbance OD range from 0.000 to 4.000 OD
• Low, Medium, High and Variable plate-shaking speeds with adjustable durations
• All models read 6-, 12-, 24-, 48-, 96- and 384-well microplates
• Injector models dispense to 6-, 12-, 24-, 48-, and 96-well microplates
• Operates from 100 to 240 V~ (± 10%) @ 50 to 60 Hz
• One serial COM port (9-pin female connector)
• One USB port
• 4-Zone incubation to 50ºC (122ºF)
• Optional dual-reagent dispensing capability
Synergy HT Operator’s Manual
4 | Chapter 1: Introduction
Package Contents
Part numbers and package contents are subject to change. Contact
BioTek Customer Care with any questions.
Item Part #
Synergy HT Operator’s Manual 7091000
injectors:
Power supply
Power cord set (specific to installation environment):
Europe (Schuko)
USA/International
United Kingdom
Australia/New Zealand
RS-232 serial cable 75034
USB cable
with USB Driver Software
Wrench 48576
Fluorescence lamp assembly (Note: The replacement lamp assembly is
PN 7080500)
Filter “plugs” (2) (also referred to as “dummy filters” or “blanks”) 7082073
Plastic storage bag and fastener strips —
Time-Resolved Fluorescence cartridge assembly (“T” models only) 7090523
Models with injectors (“SIAFRTD” “SIAFRTD-CUSTOM”), an external
dispense module (packed separately), with the following accessories:
Outlet tubes (2, plus 2 spare) from dispense module to instrument
Inlet tubes (2) from supply bottles to syringe drives
250 µl syringes (2)
Syringe thumbscrews (2)
Priming plate
Injector tip priming trough
Dispense module communication cable
Dispense module front cover
Supply bottles (2, 30 mL)
Supply bottle holder assemblies (2)
Injector tip cleaning stylus and plastic storage bag
7090204
7080501
7082120
7082121
7083000
7132158
7132169
7082137
7122609
7090564
2872304
models
with
76061
all other
models:
76077
75010
75011
75012
75013
75108
19511
75107
BioTek Instruments, Inc.
Optional Accessories | 5
Optional Accessories
Accessory availability and part numbers are subject to change. Contact
BioTek Customer Care with questions or visit www.biotek.com and use
the Accessories search tool.
Item Part #
7-filter Absorbance Test Plate
7260522
Fluorescence Test Plate
Product Qualification (IQ-OQ-PQ) package
PCR Tube Adapter Plates
Terasaki Adapter Plate
Take3 Micro-Volume Plate
Take3 Trio Micro-Volume Plate
BioCell Quartz Vessel and Adapter Plate
Additional Fluorescence Filters; contact BioTek for part numbers and availability
The Synergy HT is compatible with the BioStack Microplate Stacker. Contact BioTek or
visit our website to learn more.
7092092
7090521
6002072 and
6002076
7330531
TAKE3
Take3Trio
7272051/7270512
For Use with Liquid Tests (see Chapter 5) Part #
Absorbance Liquid Test Solutions:
BioTek Wetting Agent Solution
BioTek QC Check Solution #1
25 mL
125 mL
Dispense Module Liquid Test Solution:
BioTek Green Test Dye
BioTek Blue Test Dye
BioTek QC (Yellow) Test Dye
Individual Fluorescence Liquid Test Solutions:
Sodium Fluorescein Powder
Liquid Test Kit using Sodium Fluorescein
Liquid Test Kit using Methylumbelliferone (“MUB”)
7773002
7120779
7120782
7773003
7773001
7120782
98155
7160013
7160012
Synergy HT Operator’s Manual
6 | Chapter 1: Introduction
Product Support & Service
Technical Assistance Center (TAC)
If your instrument(s) or software fails to function properly, if you have questions about
how to use or maintain our products, or if you need to send an instrument to BioTek
for service or repair, please contact our Technical Assistance Center. BioTek’s “TAC” is
open from 8:30 AM to 5:30 PM (EST), Monday through Friday, excluding standard U.S.
holidays. You can send a fax or an e-mail any time. You can also request technical
assistance via our website:
Phone: (800) 242-4685 or Fax: (802) 654-0638 E-Mail: tac@biotek.com
(802) 655-4740
Please be prepared to provide the following information:
• Your name and company information, along with a daytime phone or fax
number, and/or an e-mail address
• The product name, model, and serial number
www.biotek.com.
Web: www.biotek.com
• The onboard software part number and version (available through Gen5 at
System > Instrument Control > Information)
• Gen5 software version information (
Help > About Gen5)
• For troubleshooting assistance or instruments needing repair, the specific steps
that produce your problem and any error codes displayed in Gen5
(see also
Appendix C, Error Codes)
Returning Instruments for Service/Repair
If you need to return an instrument to BioTek for service or repair, please contact the
TAC for a Return Materials Authorization (RMA) number before shipping the
instrument. Repackage the instrument properly (see
Chapter 2, Installation), write
the RMA number on the shipping box, and ship to BioTek.
Contacting BioTek for Applications Support
BioTek’s fully equipped Application Laboratory provides our on-staff scientists with
the means to assist you with the integration of our instrumentation and software with
your unique scientific applications. If you are having difficulty with optimizing
fluorescence sensitivity or integrating a unique data reduction transformation, or you
are just looking for a recommendation on an appropriate fluorophore, contact us.
Phone: (888) 451-5171 E-Mail: applications@biotek.com
BioTek Instruments, Inc.
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