Biotek Synergy H1 Operator's Manual
Operator’s Manual
Hybrid Multi-Mode Microplate Reader
Synergy
™
H1
Synergy H1™
Hybrid Multi-Mode Microplate Reader
Operator’s Manual
March 2014
© 2014
Part Number 8041000
Revision G
BioTek® Instruments, Inc.
ii | Notices
Notices
BioTek® Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
All Rights Reserved
© 2014, BioTek® Instruments, Incorporated. No part of this publication may be
reproduced, transcribed, or transmitted in any form, or by any means electronic or
mechanical, including photocopying and recording, for any purpose other than the
purchaser’s use without written permission of BioTek Instruments, Inc.
Trademarks
BioTek® is a registered trademark, and Synergy™ H1, Gen5™, BioStack™, and
Take3™ and Take3Trio™ Micro-Volume Plates are trademarks of BioTek Instruments,
Inc. Harta™ is a trademark of Harta Instruments. Glowell™ is a trademark of LUX
Biotechnology, Ltd.
Microsoft
®
, Windows®, and Excel® are either registered trademarks or trademarks of
Microsoft Corporation in the United States and/or other countries.
®
Kalrez
is a registered trademark of DuPont Performance Elastomers, L.L.C. (DPE).
All other trademarks are the property of their respective holders.
Restrictions and Liabilities
Information in this document is subject to change and does not represent a
commitment by BioTek Instruments, Inc. Changes made to the information in this
document will be incorporated in new editions of the publication. No responsibility is
assumed by BioTek for the use or reliability of software or equipment that is not
supplied by BioTek or its affiliated dealers.
BioTek Instruments, Inc.
Contents
Global Service and Support ................................................................ v
Revision History ............................................................................. vii
Document Conventions .................................................................... ix
Intended Use Statement .................................................................. ix
Quality Control ................................................................................ x
Warranty and Product Registration ..................................................... x
Repackaging and Shipping ................................................................. x
Warnings ........................................................................................ x
Hazards ......................................................................................... xi
Precautions ................................................................................... xii
CE Mark ....................................................................................... xiii
Electromagnetic Interference and Susceptibility ................................. xiv
User Safety ................................................................................... xv
Safety Symbols ............................................................................. xvi
Introduction ........................................................................................ 1
Product Description ......................................................................... 2
Package Contents & Accessories ........................................................ 3
Optional Accessories ........................................................................ 4
Product Support & Service ................................................................ 6
0BInstallation ......................................................................................... 7
Product Registration ........................................................................ 8
Important Information ..................................................................... 8
1: Unpack and Inspect the Reader ..................................................... 9
2: Unpack and Inspect the Dispense Module ...................................... 10
3: Unpack and Inspect the Gas Controller .......................................... 11
4: Select an Appropriate Location ..................................................... 11
5: Remove the Shipping Hardware .................................................... 12
6: Install the Power Supply .............................................................. 13
7: Connect the Gas Controller .......................................................... 13
8: Install the Dispense Module ......................................................... 13
9: Connect the Host Computer ......................................................... 17
10: Install Gen5 on the Host Computer ............................................. 18
11: Turn on the Reader ................................................................... 18
12: Establish Communication ........................................................... 18
13: Run a System Test ................................................................... 19
14: Test the Injector System ........................................................... 20
Operational/Performance Qualification ............................................... 22
Repackaging and Shipping Instructions ............................................. 22
Getting Started .................................................................................. 29
Modular Design .............................................................................. 30
External Components ...................................................................... 31
Internal Components ...................................................................... 31
Contents | iii
Synergy H1 Operator’s Manual
iv | Contents
Gen5 Software ............................................................................... 36
Recommendations for Optimum Performance ..................................... 39
Filters and Mirrors ............................................................................. 43
Filter Cube Overview ...................................................................... 44
Filters and Mirrors .......................................................................... 48
Preventive Maintenance .................................................................... 53
Preventive Maintenance .................................................................. 54
Warnings and Precautions ............................................................... 55
Clean Exposed Surfaces .................................................................. 56
Inspect/Clean Excitation and Emission Filters ..................................... 57
Inspect/Clean Mirrors ..................................................................... 58
Flush/Purge the Fluid Path ............................................................... 60
Run a Dispense Protocol (Optional) ................................................... 61
Empty/Clean the Tip Priming Trough ................................................. 62
Clean the Priming Plate ................................................................... 62
Clean the Dispense Tubes and Injectors ............................................ 63
As-Needed Maintenance .................................................................... 65
Decontamination ............................................................................ 66
Dispense Module: Syringe Replacement ............................................. 72
0BInstrument Qualification ................................................................... 75
Overview ...................................................................................... 77
IQ/OQ/PQ ..................................................................................... 77
Recommended Qualification Schedule ............................................... 78
System Test .................................................................................. 79
Absorbance Plate Test ..................................................................... 85
Absorbance Liquid Tests .................................................................. 91
Fluorescence Liquid Tests ................................................................ 99
Luminescence Test ....................................................................... 122
Dispense Module Tests .................................................................. 129
Specifications .................................................................................. 141
General Specifications ................................................................... 142
Absorbance Specifications ............................................................. 144
Dispense/Read Specifications ......................................................... 145
Fluorescence Specifications (Mono-Based) ....................................... 145
Fluorescence Specifications (Filter-Based) ........................................ 146
Luminescence Specifications .......................................................... 147
Error Codes ..................................................................................... 149
Overview .................................................................................... 150
Error Codes ................................................................................. 151
Instrument Dimensions for Robotic Interface ................................. 155
BioTek Instruments, Inc.
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