Biotek MultiFlo FX Operator's Manual

Operator’s Manual

Microplate Dispenser

MultiFlo™ FX Microplate

Dispenser



MultiFlo™ FX Multi-Mode Dispenser
Operator's Manual

BioTek Instruments, Inc.
May 2014
© May 2014
Part Number 1261000
Revision B

ii | MultiFlo FX Operator's Manual

Notices

BioTekInstruments, Inc.

Highland Park, P.O. Box 998

Winooski, Vermont 05404-0998 USA

All Rights Reserved

© 2014, BioTek®Instruments, Incorporated. No part of this publication may be
reproduced, transcribed, or transmitted in any form, or by any means electronic
or mechanical, including photocopying and recording, for any purpose other
than the purchaser’s use without written permission of BioTek Instruments, Inc.

Trademarks

BioTek®is a registered trademark and MultiFlo™ FX Multi-Mode Dispenseris a
trademark of BioTek Instruments, Inc.

Microsoft®, Internet Explorer®, Windows®, Windows 7, Windows 8, Windows
Vista®, Windows XP, and Excel®are either registered trademarks or trademarks
of Microsoft Corporation in the United States and/or other countries.

All other trademarks are the property of their respective holders.

Restrictions and Liabilities

Information in this document is subject to change and does not represent a
commitment by BioTek Instruments, Inc. Changes made to the information in
this document will be incorporated in new editions of the publication. No
responsibility is assumed by BioTek for the use or reliability of software or
equipment that is not supplied by BioTek or its affiliated dealers.

MultiFlo™ FX Multi-Mode Dispenser

Notices|iii

Contents

Notices ii

All Rights Reserved ii
Trademarks ii
Restrictions and Liabilities ii

Global Service and Support ix
Revision History x
Document Conventions xi
Intended Use Statement xi
Quality Control xi
Warranty and Product Registration xii
Dispense Cassette Warranty xii
Repackaging and Shipping xii
Warnings xii
Hazards and Precautions xiii

Hazards xiii
Precautions xiv

CE Mark xvi

Directive 2004/108/EC: Electromagnetic Compatibility xvi
Directive 2006/95/EC Low Voltage (Safety) xvi
Directive 2002/96/EC: Waste Electrical and Electronic Equipment xvii
Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use) xvii

Electromagnetic Interference and Susceptibility xvii

USA FCC CLASS A xvii
Canadian Department of Communications Class A xviii

User Safety xviii
Safety Symbols xix

Introduction 1

Introducing the MultiFlo™ FX Dispenser 2
Features of the MultiFlo FX 3

Washer 3
Peri-pump Dispenser 4
Syringe Dispensers 4
Liquid Handling Control™ (LHC) Software 5
RAD™ Technology Features 5
MultiFlo FXModels 6

MultiFlo FX Dispenser Comparison 7

Plate Types Table 9

BioStack Compatibility 10
Package Contents 12
Optional Accessories 13

Peri-pump Optional Accessories 13
Syringe Dispenser Optional Accessories 14

BioTek Instruments, Inc.

iv | MultiFlo FX Operator's Manual

Strip Washer Optional Accessories 15

Physical Specifications 18
Performance Specifications 21

Peri-Pump Dispenser 21
Syringe Dispensers 22
Strip Washer 23

Space Requirements 25
BioTek's Customer Resource Center 26

Installation 27

Unpack and Inspect the Instrument 28
Install the Waste Tubing 29
Remove the Shipping Hardware 31
Set up the Strip Washer 34

Strip Washer accessories 34
Attach washer module and connect its cable 35
Install the tubing bracket 37
Install the Fluid Supply 38
Install the Waste Bottle 39
Install the muffler 41
Install Washer Manifolds 41

Set Up the Peri-pump Dispenser 44

Attach the Peri-pump Cover Panel 44

Install the Secondary Peri-Pump 46
Install RAD Technology Components 51

Install the Tubing Bracket and RADModule 51
Install the tubing bracket 52
Attach the RADModule to the Tubing Bracket 52
Install RAD Dispense Head 53
Dispense Cassette Diagram 54
Install the Dispense Cassette 56
Install RAD Technology Cassette 58
Prime Trough Inserts 59
Peri-pump Reservoir Holder 60
Shorten the Dispense Cassette Tubing 61

Install the Syringe Dispenser Component 62

Syringe Dispenser Placement Options 63
Install the Syringe Dispenser on Back Shelf 64
Install the Syringe Dispenser on Top 65
Install Tubing and Manifolds for Syringe Dispenser 68
Syringe Dispenser Check Valves 70
Install Inline Filter for 32-Tube Dispensers 71

Install Software/Connect to Computer 71

Connect to Host Computer 72

Connect Power Cable 72
Define Instrument Settings 73

LHC Users Only 73
Enable Waste Sensor 73

MultiFlo™ FX Multi-Mode Dispenser

Notices|v

Update the Instrument to use the Syringe Dispenser 74
Change the Syringe Dispenser Manifold 75
Change the cassette type setting 76
Setting the Time and Date 77
Define Startup Preferences (LHC users only) 77

Verify Performance 79

Verify the Peri-Pump Dispenser 80
Verify the Syringe Dispensers 80
Verify the Washer Component 81

Repacking the MultiFlo FX 82
Install the Shipping Hardware 83
Repacking the Secondary Peri-pump 85
MultiFlo FX Repacking 86
Repacking the Syringe Dispenser 89
Repacking the Strip Washer 90

Operation 91

Touch Screen Basics 92

Quick Prime 93
Quick Dispense 94
Quick Wash 95
Add protocols to the Home screen 95
Remove protocols from the Home screen 95
Get protocols from a memory stick - USB drive 96
Hardware Change Detected 96

Optimize Performance 98

Recommended prime volumes for the Strip Washer 99
Recommended prime volumes for the Peri-pump 99
Recommended prime volumes for the Syringe dispensers 101
Optimize protocols to improve evacuation 101
Add a secondary aspiration to a wash cycle 101

Create or Modify a Protocol 102
Protocol Parameters Tables 103
Wash Parameters Table 108

Washer Flow Rates 111
Pre-Dispense 113
Minimum Prime Volumes for the Strip Washer 114
Bottom Wash 114
Change the Plate Type 115
Rename a protocol 115
Shake/Soak Step Parameters 116
Repeat steps in a Loop 116
Delay the Protocol 117
Timer Activated Delay 118
RUN: Running Predefined Protocols 120
LHC Users Only: Customize the Predefined Protocols 120

Predefined Protocols Listing 121
Predefined Sample Protocols 122
Operating with the BioStack 124

BioTek Instruments, Inc.

vi | MultiFlo FX Operator's Manual

Use the BioStack 125
Re-stack plates in the BioStack 127
Nth Plate to perform special process 127
About the Nth Plate Step 128

Advanced Plate Sequencing with the BioStack 129
Plate Types and Processing Patterns 133
Handling Special Plates and Mini-tubes 134

1536F - 1536-well Flanged Plates 134
Peri-pump Special Plate Handling 134
Special Plate Carrier for Mini-tubes 136
Mini-Tube Racks 137
Dispensing to Mini-Tubes 137

Dispense Processing Patterns 138

8-Channel Dispenser Dispense Pattern 139
Dispense Pattern with Both Syringes 140

Washer Operation 141

About the MultiFlo FX Wash Step 141

Cell Wash 142

Define a Cell Wash Protocol 142
Cell Wash Strategies 143

Biomagnetic Separation - Magnetic Bead Assays 145

Perform Magnetic Bead Assays 146
Optimize Magnetic Bead Protocols 147
Flat Magnets 147
Ring Magnets 148
How to determine the Magnet Adapter Height Offset 149
Magnet Height Offset 150
Special Procedure for Magnetic Bead Assays 151

Peri-pump Peristaltic Dispenser 152
Peri-pump: How it works 153

Release the tension on the dispense cassette 154

Peri-pump Dispense Step 155

RADTip Tracking 156
RAD Well Map 157

Require a Specific Peri-pump Cassette 158
Peri-pump Advanced Settings 159

Settings for 0.5 µL Dispense 159
8-to-1 Tube RAD Cassette 159

Dual Syringe Dispenser 160

How to Prime the Syringe dispenser 160

Syringe Prime Step 161
Syringe Dispense Step 163

Change the Syringe Dispenser Manifold 165

Syringe Dispenser Settings 166
Syringe Dispenser- Autoclavable vs. Non-autoclavable 167
Changing the Instrument's Settings 168

Dispense Pattern 168

MultiFlo™ FX Multi-Mode Dispenser

Notices|vii

Change the Plate Carrier Setting (Touch screen) 169
Change the Plate Clearance Setting (Touch screen) 169
Plate Carrier Speed 170
Upload-Download Protocols (LHC Only) 170
Transfer Protocols (Touch Screen Only) 170
Transfer Plate Lid Definitions 171

Maintenance 173

Overview 174
Recommended Maintenance Schedule 176
Daily Maintenance 178
Overnight/Multi-Day Maintenance 179
AutoPrime 179

Turn on AutoPrime for the Peri-pump Dispenser 180
Turn on AutoPrime for the Syringe Dispenser 182
Turn on AutoPrime for the Washer 183

Soak the manifold tubes in cleaning fluid 185
Removing Protein Residuals and Fungi Growth 187
Periodic Maintenance 189

Autoclavable Components 190
Clean the Bottles 190
Clean the Plate Carrier 191
Clean the exterior surfaces 191

Peri-pump Dispenser Maintenance 192

Flush the Dispense Cassette 192
Unclog the Dispense Tips 194
RADSingle-tube Cassette - Unclog the Tip 196
RAD8-to-1 Cassette - Unclog the Tips 197
Record the Number of Plates Processed 199
RAD8-to-1 Cassette - Replace Chute 200

Syringe Dispenser and Strip Washer Maintenance 201

Clean the Manifolds 201
Clean the Dispense/Aspirate Tubes 202
Clean or Replace the Check Valves 204
Autoclave the Syringe Head 204

Decontamination 205

Tools and Supplies 206
Decontaminate Exterior Surfaces 207
Decontaminate Tubing and Manifold 208

Long Shutdown (Prepare for Storage or Shipment) 208

Storing the Instrument 210

Replace Components 210
Calibrate the Backlash for Syringe Dispenser 212

Qualification 215

Qualification Overview 216

Washer 216
Peri-pump and Syringe Dispensers 216

Qualification Schedule 217

BioTek Instruments, Inc.

viii | MultiFlo FX Operator's Manual

Index..........................................................................................

System Self-Test, Verify Information 218

Record Onboard Software 218

Liquid Testing the MultiFlo™ FX Multi-Mode Dispenser 219

Which Tests to Perform? 219
Important Recommendations for All Liquid Tests 220

Peri-pump Dispense Precision and Accuracy Tests 221

Peri-pump Dispenser Test Materials 223
Peri-pump Dispenser Precision and Accuracy Test Solutions 223

Perform the Peri-Pump Precision and Accuracy Tests 224
Syringe Dispenser Liquid Tests 235

Syringe Dispenser Test Materials 236
Syringe Dispenser Test Solutions 236

Perform the Syringe Dispense Precision & Accuracy Test 236
Washer Qualification Liquid Tests 250

Dispense Precision and Accuracy Test 250
Evacuation Efficiency Test 250
Washer Qualification Test Materials 251
Create Washer Qualification Protocols 252
Dispense Precision and Accuracy Test 253
Evacuation Efficiency Test 254
Evacuation Diagnostic Test 256

Troubleshooting 271

Troubleshooting 272

Syringe Dispenser Troubleshooting 273
Dispense Manifold Movement 273
Fluid Delivery 274
Fluid Leakage 276
Microplate Carrier Movement 276
Syringe Movement 277
Peri-pump Troubleshooting 277
Washer Troubleshooting 278
Fluid Aspiration 278
Fluid Delivery 280
Fluid Leakage 282

Error Codes 283

System Error Codes 284
MultiFlo FX Software Error Codes 294

Chemical Compatibility 306

301

MultiFlo™ FX Multi-Mode Dispenser

Global Service and Support| ix

Global Service and Support

BioTek instrument service and repair is available worldwide at several of BioTek’s
International Service Centers and in the field at your location. Contact the office
nearest you to arrange service or to get answers to your technical questions, call the
Technical Assistance Center (TAC) at 802-655-4740 in the US.

BioTek World Headquarters US BioTek China

Mailing Address:
PO Box 998, Highland Park
Winsooki, VT 05404-0998 United States

Service Shipping Address:
15 Tigan Street
Winooski, VT 05404 United States

Phone: [phone]
Fax: [phone]

Email: [e-mail]
Website: www.biotek.com

BioTek Germany Service Center
& European Coordination Center

Kocherwaldstrasse 34 D-74177
Bad Friedrichshall
Germany

Phone: [phone]
Fax: [phone]

Room 304, Tower D
Ocean International Center
62 Middle 4th East Ring Road
Chaoyang District
Beijing 100024
P.R. China

Phone: [phone]
Fax: [phone]

Email: [e-mail]
Website: www.biotekchina.com.cn

BioTek Instruments SAS

Bureau de liaison France

50 avenue d’Alsace
68025 Colmar Cedex
France

Phone: [phone]
Fax: [phone]

Email: [e-mail]
Website: www.biotek.de

Email: [e-mail]
Website: www.biotek.fr

BioTek India BioTek Singapore

Unit 223, Linkway Estate
New Link Road, Malad West
Mumbai 400064
India

Phone: [phone]
Fax: [phone]

Email: [e-mail] Email: [e-mail]

20 Science Park Road #01-08A
Teletech Park
Singapore 117674

Phone: [phone]
Fax: [phone]

BioTek Instruments, Inc.

x | MultiFlo FX Operator's Manual

BioTek India BioTek Singapore

Website: www.biotek.in Website: www.biotek.com

BioTek South Korea BioTek Switzerland

3F, Gyungnam building, 830-48
Yeoksam-dong, Gangnam-gu
Seoul, South Korea (135-936)

Phone: [phone]
Fax: [phone]

Email: [e-mail]
Website: www.biotekinstruments.co.kr

Zentrum FanhÖfli 8
6014 Luzern
Switzerland

Phone: [phone]
Fax: [phone]

Email: [e-mail]
Website: www.biotek.ch

BioTek United Kingdom (UK) BioTek Japan

6 Bull Street
Potton, Bedfordshire SG19 2NR
United Kingdom

Phone: [phone]
Fax: [phone]

Email: [e-mail]
Website: www.biotek.uk.com

8F Andoh Building
2-7-1 Taito, Taito-ku
Tokyo 110-0016 Japan

Phone: [phone]
Fax: [phone]

Email: [e-mail] Website:
www.biotek.com/ja

Revision History

Rev Date Changes

A 10/2013 First Issue

B 5/2014 The manual was updated to add RAD Technology content

throughout. Syringe dispenser installation steps were updated to
add the "Import from USB" calibration data feature. Added
references to new Peri-pump cassette types and new Syringe
dispenser manifolds. Added references to and some instructions
for running the BioStack 4 to process microplates with lids.
Updated the contents of the Cassette Calibration folder to match
improvements made to its separately purchased accessory kit:
PN7170017. Refined the procedure to remove proteins from the
tubing by emphasizing the need to flush first with buffer.

MultiFlo™ FX Multi-Mode Dispenser

Document Conventions

This manual uses the following typographic conventions:

n This note format calls attention to important information.

Warnings are presented in this style to call attention to potential hazards and

other safety concerns.

This icon calls attention to important safety information.

Document Conventions| xi

Tips and suggestions for improving performance are formatted this way.

Navigation instructions: how to get to the function being described

Intended Use Statement

l The MultiFlo™ FX Multi-Mode Dispenser provides microplate priming and dispensing, and

when equipped, washing, for ELISA™, fluorescence and chemiluminescence
immunoassays, cellular and agglutination assays.

l If the instrument has an "IVD" label it may be used for clinical and non-clinical purposes,

including research and development. If there is no such label the instrument may only be
used for research and development and non-clinical purposes.

Quality Control

It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for
the test to be conducted. Failure to conduct Quality Control checks could result in
erroneous test data.

BioTek Instruments, Inc.

xii | MultiFlo FX Operator's Manual

Warranty and Product Registration

Please take a moment to review the Warranty information that shipped with your
product. Please also register your product with BioTek to ensure that you receive
important information and updates about the product(s) you have purchased.

You can register online through BioTek’s Customer Resource Center (CRC) at

www.biotek.com or by calling 888/451-5171 or 802/655-4740.

Dispense Cassette Warranty

The Peri-pump dispense cassettes are backed by BioTek's Confidence-Plus lifetime
warranty. For the lifetime of the cassette, BioTek guarantees the cassette
components (Tip Holder, Center Holder, Tube Tensioner, and Tube Organizer) will
withstand steam autoclave conditions of 121°C and 1 bar (750 mmHg) without
adversely affecting dispense performance. In the event of a failure of any cassette
component previously listed, BioTek will replace the cassette components free of
charge.

Repackaging and Shipping

If you need to ship the instrument to BioTek for service or repair, contact BioTek
for a Return Materials Authorization (RMA) number and use the original packing
materials. Other forms of commercially available packaging are not recommended
and can void the warranty. If the original packing materials have been damaged or
lost, contact BioTek for replacement packing.

Warnings

Operate the instrument on a level, stable surface away from excessive
humidity.
When operated in a safe environment, according to the instructions in this
document, there are no known hazards associated with the MultiFlo FX.
However, the operator should be aware of certain situations that could result in
serious injury; these vary depending on the instrument type. See Hazards and

Precautions.

Strict adherence to instrument maintenance and qualification procedures is
required to ensure accurate dispense volumes and risk-free operation.

MultiFlo™ FX Multi-Mode Dispenser

Hazards and Precautions

Hazards

The following hazards are provided to help avoid injury:

Warning! Power Rating. The instrument’s power supply or power cord must
be connected to a power receptacle that provides voltage and current within the
specified rating for the system. Use of an incompatible power receptacle may
produce electrical shock and fire hazards.

Warning! Electrical Grounding. Never use a plug adapter to connect primary
power to the external power supply. Use of an adapter disconnects the utility
ground, creating a severe shock hazard. Always connect the power cord directly
to an appropriate receptacle with a functional ground.

Warning! Service. Only qualified technical personnel should perform service
procedures on internal components.

Hazards and Precautions| xiii

Warning! Accessories. Only accessories which meet the manufacturer’s
specifications shall be used with the instrument.

Warning! Lubricants. Do not apply lubricants to the microplate carrier or
carrier track. Lubricant on the carrier mechanism will attract dust and other
particles, which may obstruct the carrier path and cause the instrument to
produce an error.

Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into
internal components creates a potential for shock hazard or instrument
damage. If a spill occurs while a program is running, abort the program and
turn the instrument off. Wipe up all spills immediately. Do not operate the
instrument if internal components have been exposed to fluid.

Warning! Unspecified Use. Failure to operate this equipment according to
the guidelines and safeguards specified in this manual could result in a
hazardous condition.

Warning! Software Quality Control. The operator must follow the
manufacturer’s assay package insert when modifying software parameters and
establishing washing or dispensing methods. Failure to conduct quality

control checks could result in erroneous test data.
Warning! Internal Voltage. Always turn off the power switch and unplug the

power supply before cleaning the outer surface of the instrument.

BioTek Instruments, Inc.

xiv | MultiFlo FX Operator's Manual

Warning! Potential Biohazards. Some assays or specimens may pose a
biohazard. Adequate safety precautions should be taken as outlined in the
assay’s package insert. This hazard is noted by the symbol shown here. Always
wear safety glasses and appropriate protective equipment, such as chemically
resistant rubber gloves and apron.

Warning! Pinch Hazard. Some areas of the instrument or its components can
present pinch hazards when the instrument is operating. Depending on the
instrument or component, these areas are marked with the symbol shown here.
Keep hands/fingers clear of these areas when the instrument is operating.

Precautions

The following precautions are provided to help avoid damage to the instrument:

Caution: Service. The instrument should be serviced by BioTek authorized
service personnel. Only qualified technical personnel should perform
troubleshooting and service procedures on internal components.

Caution: Spare Parts. Only approved spare parts should be used for
maintenance. The use of unapproved spare parts and accessories may result in
a loss of warranty and potentially impair instrument performance or cause
damage to the instrument.

Caution: Environmental Conditions. Do not expose the instrument to
temperature extremes. For proper operation, ambient temperatures should
remain within the range listed in the Specifications section. Performance may
be adversely affected if temperatures fluctuate above or below this range.
Storage temperature limits are broader.

Caution: Sodium Hypochlorite. Do not expose any part of the instrument to
the recommended diluted sodium hypochlorite solution (bleach) for more than
20 minutes. Prolonged contact may damage the instrument surfaces. Be certain
to rinse and thoroughly wipe all surfaces.

Caution: Buffer Solution. Although many precautions have been taken to
ensure that the instrument is as corrosion-proof as possible, the instrument is
not sealed and liquids can seep into sensitive components. Make sure that any
spilled buffer solution is wiped off the instrument. Prolonged exposure to salt
solution may corrode parts of the microplate carrier, movement rail, springs,
and other hardware.

Caution: Chemical Compatibility. Some chemicals may cause irreparable
damage to the instrument. The following chemicals have been deemed safe for
use in the instrument: buffer solutions (such as PBS), saline, surfactants,
deionized water, 70% ethyl, isopropyl, or methyl alcohol, 40% formaldehyde,
and 20% sodium hydroxide. Never use acetic acid, DMSO, or other organic
solvents. These chemicals may cause severe damage to the instrument.

MultiFlo™ FX Multi-Mode Dispenser

Hazards and Precautions| xv

Contact BioTek for more information and prior to using other questionable
chemicals.

Caution: Bovine Serum Albumin. Solutions containing proteins, such as
bovine serum albumin (BSA), will compromise the instrument’s performance
over time unless a strict maintenance protocol is adhered to. See
Maintenance procedures regarding BSA.

Caution: Power Supply. Only use the power supply shipped with the
instrument. Operate this power supply within the range of line voltages listed
on it.

Caution: Disposal. This instrument contains printed circuit boards and wiring
with lead solder. Dispose of the instrument according to Directive 2002/96/EC,
“on waste electrical and electronic equipment (WEEE),” or local ordinances.

Caution: Warranty. Failure to follow preventive maintenance protocols may
void the warranty.

Caution: Shipping Hardware. All shipping hardware (e.g., shipping bracket

etc.) must be removed before operating the instrument and reinstalled before
repackaging the instrument for shipment.

Caution: Do not run the Peri-pump without a cassette installed on the pump.
Caution: Electromagnetic Environment. Per IEC 61326-2-6 it is the user’s

responsibility to ensure that a compatible electromagnetic environment for this
instrument is provided and maintained in order that the device will perform as
intended.

Caution: Electromagnetic Compatibility. Do not use this device in close
proximity to sources of strong electromagnetic radiation (e.g., unshielded
intentional RF sources), because these may interfere with the proper operation.

BioTek Instruments, Inc.

xvi | MultiFlo FX Operator's Manual

CE Mark

Based on the testing described below and information contained
herein, this instrument bears the CE mark.

n Note: See the Declaration of Conformity for specific information.

Directive 2004/108/EC: Electromagnetic Compatibility

Emissions—Class A

The system has been type-tested by an independent, accredited testing
laboratory and found to meet the requirements of EN 61326-1: Class A for
Radiated Emissions and Line Conducted Emissions.

Verification of compliance was conducted to the limits and methods of EN 55011
(CISPR 11) Class A. In a domestic environment it may cause radio interference,
in which case, you may need to mitigate the interference.

Immunity

The system has been type-tested by an independent, accredited testing
laboratory and found to meet the requirements of EN 61326-1 and EN 61326-2-6
for Immunity. Verification of compliance was conducted to the limits and
methods of the following:

EN 61000-4-2, Electrostatic Discharge

EN 61000-4-3, Radiated EM Fields

EN 61000-4-4, Electrical Fast Transient/Burst

EN 61000-4-5, Surge Immunity

EN 61000-4-6, Conducted Disturbances from RFI

EN 61000-4-11, Voltage Dips, Short Interruptions and Variations

Directive 2006/95/EC Low Voltage (Safety)

The system has been type-tested by an independent testing laboratory and was
found to meet the requirements of this Directive. Verification of compliance was
conducted to the limits and methods of the following:

MultiFlo™ FX Multi-Mode Dispenser

Electromagnetic Interference and Susceptibility| xvii

EN 61010-1, “Safety requirement for electrical equipment for measurement,
control and laboratory use. Part 1, General requirements.”

EN 61010-2-081, “Particular requirements for automatic and semi-automatic
laboratory equipment for analysis and other purposes.”

Directive 2002/96/EC: Waste Electrical and Electronic Equipment

Disposal Notice: This instrument contains printed circuit boards and wiring with
lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on
waste electrical and electronic equipment (WEEE)” or local ordinances.

Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use)

l Product registration with competent authorities.
l Traceability to the U.S. National Institute of Standards and Technology (NIST).

EN 61010-2-101 Particular requirements for in vitro diagnostic (IVD) medical
equipment.

Electromagnetic Interference and Susceptibility

USA FCC CLASS A

RADIO AND TELEVISION INTERFERENCE

NOTE: This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits
are designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if not installed
and used in accordance with the instruction manual, may cause harmful
interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference, in which case the user
will be required to correct the interference at their own expense.

In order to maintain compliance with FCC regulations shielded cables must be
used with this equipment. Operation with non-approved equipment or
unshielded cables is likely to result in interference to radio and television
reception.

BioTek Instruments, Inc.

xviii | MultiFlo FX Operator's Manual

Canadian Department of Communications Class A

This digital apparatus does not exceed Class A limits for radio emissions from
digital apparatus set out in the Radio Interference Regulations of the Canadian
Department of Communications.

Le present appareil numerique n'émet pas de bruits radioelectriques depassant
les limites applicables aux appareils numerique de la Class A prescrites dans
le Reglement sur le brouillage radioelectrique edicte par le ministere des
Communications du Canada.

User Safety

This device has been type-tested by an independent laboratory and found to meet
the requirements of the following:

l Underwriters Laboratories UL 61010-1 “Safety requirements for electrical

equipment for measurement, control and laboratory use; Part 1: general
requirements.”

l Canadian Standards Association CAN/CSA C22.2 No. 61010-1 “Safety

requirements for electrical equipment for measurement, control and
laboratory use; Part 1: general requirements.”

l EN 61010 Standards, see CE Mark on page xvi.

MultiFlo™ FX Multi-Mode Dispenser

Safety Symbols

Some of these symbols appear on the instrument or accessories:

Alternating current
Courant alternatif
Wechselstrom
Corrientealterna
Correntealternata

Safety Symbols| xix

Both direct and alternating current
Courant continu et courant alternatif
Gleich - und Wechselstrom
Corriente continua y corrientealterna
Corrente continua e
correntealternata

Direct current
Courant continu
Gleichstrom
Corriente continua
Corrente continua

On (Supply)
Marche (alimentation)
Ein (VerbindungmitdemNetz)
Conectado
Chiuso

Off (Supply)
Arrêt (alimentation)
Aus (TrennungvomNetz)
Desconectado
Aperto
(sconnessionedallaretedialimentazio
ne)

Warning, risk of electric shock
Attention, risque de choc électrique
Gefährlicheelektrischeschlag
Precaución, riesgo de
sacudidaeléctrica
Attenzione, rischiodiscossaelettrica

Earth ground terminal
Borne de terre
Erde (Betriebserde)
Borne de tierra
Terra (difunzionamento)

Protective conductor terminal
Borne de terre de protection
Schutzleiteranschluss
Borne de tierra de protección
Terra diprotezione

Caution (refer to accompanying
documents)
Attention (voir documents
d’accompanement)
AchtungsieheBegleitpapiere
Atención (vease los
documentosincluidos)
Attenzione, consultare la doc annessa

Warning, risk of crushing or pinching
Attention, risqued’écrasement et
pincement
Warnen, Gefahr des Zerquetschens
und Klemmen
Precaución, riesgo del
machacamiento y sejeción
Attenzione,
rischiodischiacciareedintrappolarsi

Warning, hot surface
Attention, surface chaude
Warnen, heißeOberfläche
Precaución, superficiecaliente
Attenzione, superficiecalda

In vitro diagnostic medical device
Dispositif médical de diagnostic in
vitro
Medizinisches In-Vitro-Diagnostikum
Dispositivo médico de diagnóstico in

Warning, potential biohazards
Attention,
risquesbiologiquespotentiels
Warnung!
MoeglichebiologischeGiftstoffe
Atención, riesgosbiológicos
Attenzione, rischiobiologico

Separate collection for electrical and
electronic equipment
Les équipements électriques et
électroniques font l’objet d’une
collecte sélective

BioTek Instruments, Inc.

xx | MultiFlo FX Operator's Manual

vitro
Dispositivo medico diagnostico in
vitro

Consult instructions for use
Consulter la notice d’emploi
Gebrauchsanweisung beachten
Consultar las instrucciones de uso
Consultare le istruzioni per uso

Getrennte Sammlung von Elektro­und Elektronikgeräten
Recogida selectiva de aparatos
eléctricos y electrónicos
Raccolta separata delle
apparecchiature elettriche ed
elettroniche

MultiFlo™ FX Multi-Mode Dispenser

Chapter 1

Introduction

Thank you for purchasing the MultiFlo™ FX Multi-Mode
Dispenser. This chapter describes the instrument's features
and specifications and includes important contact information.

Introducing the MultiFlo™ FX Dispenser 2
Features of the MultiFlo FX 3
MultiFlo FX Dispenser Comparison 7
BioStack Compatibility 10
Package Contents 12
Optional Accessories 13
Physical Specifications 18
Performance Specifications 21
Space Requirements 25
BioTek's Customer Resource Center 26

2 | Chapter 1: Introduction

Introducing the MultiFlo™ FX Dispenser

The MultiFlo FX offers several devices in one instrument: one or two peristaltic
pump dispensers called the Peri-pump, a dual Syringe pump dispenser, and either
a strip Washer or a single-channel Random Access Dispenser (RAD) module. The
MutliFlo FX is available in multiple configurations to support numerous
microplate geometries.

Device/Component Description

1 Peri-pump Dispenser Peristaltic, 8-channel dispenser with entirely visible fluid

path.

2 Dispense arm Holds the Peri-pump Tip Holder(s), the Syringe dispenser

manifolds and the washer dispense manifold, when
applicable.

MultiFlo™ FX Multi-Mode Dispenser

Features of the MultiFlo FX | 3

Device/Component Description

3 Aspirate/RAD arm Washer and RAD models only: holds the strip washer's

aspirate manifold or the RAD cassette's tip holder.

4 Priming trough Waste reservoir for collecting or disposing of priming fluid.

Priming trough inserts, for collecting fluid, are shown in this
image.

5 Plate Carrier Holds standard microplates for processing.

6 Touch screen One of two ways to control the MultiFlo FX; the other way is

LHC.

7 Strip Washer An external module containing a syringe-pump dispenser

and vacuum aspiration pump.

8 Syringe Dispenser Another external module with two distinct syringe-pump

dispensers that support a variety of manifold types to
process standard and low-density microplates.

9 Peri-pump Dispenser Secondary or external Peri-pump dispenser (required for

RAD models).

See Plate Types Table on page 9 to review the plate types supported by each

device.

Features of the MultiFlo FX

l Supports all microplate-based assays, including ELISA, fluorescence,

chemiluminescence, RIA, DNA probes, and cellular assays.

l A variety of solutions, including buffered saline and reagents can be dispensed.

l The intuitive onboard software allows you to create and store wash and dispense

protocols. BioTek provides numerous predefined protocols for maintenance and
instrument qualification purposes.

l Two USB flash drive ports facilitate file transfer and storage.

l A low-maintenance design, the result of BioTek’s long history with liquid-handling

instruments.

l Compatible with BioTek’s BioStack™ Microplate Stacker for automated plate processing.

l A robot-accessible carrier that can be interfaced into some robotic systems.

l Computer control using BioTek’s Liquid Handling Control™software (“LHC”).

Washer

BioTek Instruments, Inc.

4 | Chapter 1: Introduction

l Programmable dispense volumes, and a wide range of wash options, from gentle

washing for cellular assays to vigorous washing for ELISA.

l A “bottom washing” routine to lower the background absorbance and “crosswise” or

secondary aspiration to reduce residual volumes.

l Supports Wash, Prime, Dispense, and Aspirate steps.

l Several predefined protocols are provided to simplify preventative maintenance, which

should be performed regularly to ensure optimum washer performance.

Peri-pump Dispenser

l A peristaltic pump with eight individual tubes transfers fluid from a supply bottle, or up

to eight different supply bottles, to various vessels. The pump has four rollers over which
the tubing is stretched.

l The tubing is contained in an easy to load and unload cassette that is attached to the

pump head. The pump’s protective cover must be in place to run a dispense routine.

l Three tubing sizes are available: 1 µL, 5 µL, and 10 µL for the most precise dispensing of

volumes from 1 to 30,000 µL and 0.5 µL dispenses when using a 1 µL cassette.

l RAD™ Technology models support "random access dispensing" with several single-

channel cassette options, including a bulk dispensing cassette (8 tubes to one chute) with
flow tracking to minimize turbulence in the wells.

l Autoclavable tubing (steam temperatures and pressures of 121° C and 1 bar

(750 mmHg)) is compatible with 70% ethyl or isopropyl alcohol and 0.5% sodium
hypochlorite (bleach) solution for easy maintenance.

Syringe Dispensers

l The Syringe dispenser has a long-lasting seal that ensures precise and accurate fluid

delivery, as well as reproducibility for repeated dispenses.

l Two syringe pumps support distinct fluid sources and dispense manifolds:

o

16-channel: one tube per well for 384-well plates and two tubes per well for 96­well plates.

o

32-channel: one tube per well for 1536-well plates.

o

8-channel (two manifolds in one block): one tube per well for both 96- and 384­well plates.

l Autoclavable components can be used with organic solvents and provide easy

maintenance.

l Does not require recalibration

Low-density Microplate-specific Syringe Manifolds

Plate-specific dispense manifolds with angled dispensing for cellular assays:

MultiFlo™ FX Multi-Mode Dispenser

Features of the MultiFlo FX | 5

l 6-well plate: 8-channel dispense manifold (4 tubes/well).

l 12-well plate: 9-channel dispense manifold (3 tubes/well).

l 24-well plate: 8-channel dispense manifold (2 tubes/well).

l 48-well plate: 12-channel dispense manifold (2 tubes/well).

Liquid Handling Control™ (LHC) Software

BioTek’s Liquid Handling Control (LHC) software lets you control the instrument
from your computer. You’ll enjoy the convenience of programming assay-specific
wash and dispense protocols in a familiar Windows environment (Microsoft
Windows®7, Windows 8, and Windows XP).

For high-throughput applications, the LHC supports BioStack™ integration.

Please refer to the LHC Installation Guide and Help system to learn about:

o

Installing the LHC software on the controlling computer

o

Running Maintenance protocols

®

o

Running Qualification protocols

o

Special considerations when operating with the BioStack Microplate Stacker

RAD™ Technology Features

Random Access Dispensing or RAD models offer the most gentle dispensing
possible to support sensitive cellular assays. The single-tube cassettes are ideal for
reagent preservation, hit-picking or partial plate filling. The 8-to-1 channel bulk ­dispensing cassettes are designed to preserve cell monolayers in low-density
plates. An external Peri-pump powers the RAD cassettes, which are not compatible
with the internal Peri-pump.

At a glance:

Features Single-tube 8-to-1 tube

Plate
Types

Cassette
tubing

6- to 384-well 6-, 12-, 24-well

1 µL, 5 µL, or 10 µL 5 µL only

Primary
Uses

Example
Application

Random dispensing in 48-,
96-, and 384-well plates

Dispensing costly reagent ­lowest dead volume

Fast but gentle dispensing with "Tip Tracking"

Media addition to cells in low-density plates

BioTek Instruments, Inc.

6 | Chapter 1: Introduction

Features Single-tube 8-to-1 tube

Other Hit picking: fast, intuitive

selection of individual
wells, rows, or columns;
Access and preserve more
reagent: single tube can
handle minute quantities of
reagent.

MultiFlo FXModels

Tip Tracking: gradually raises the tip chute as
fluid is dispensed to the well to ensure the least
disturbance possible. See RADTip Tracking on

page 156.

cell monolayer is preserved

Model Internal Peri-pump Strip Washer RAD Technology

MFX

MFXW X

MFXP X

MFXPW X X

MFXR X

MFXPR X X

The Syringe and secondary Peri-pump dispensers are ordered separately. Except
RAD models include the external Peri-pump.

MultiFlo™ FX Multi-Mode Dispenser

MultiFlo FX Dispenser Comparison | 7

MultiFlo FX Dispenser Comparison

Counting the washer as a potential dispenser, the MultiFlo FX offers three distinct
dispensers. Here is a comparison of the devices:

n For precious reagents use the Peri-pump to preserve unused fluids. It has

the shortest, most visible fluid path, and a Purge capability to reverse the fluid
flow to recover fluid from the tubing. Another advantage is the ability to
dedicate a dispense cassette to use with one reagent only, reducing the
amount of priming required prior to use. And, you can shorten the tubing to
further reduce the dead volume: See Shorten the Dispense Cassette

Tubing on page 61.

n RAD Technologyoffers the best tools for preserving cells and reagents.

n All MultiFlo FX devices are capable of dispensing up to 30,000 µL. BioTek's

recommended maximum dispense volumes are cited here.

Volume

Device

range
µL/well

Peri-pump 0.5‡, and

1-3000*

RAD
Technology

0.5‡, and
1­30,000*

Syringe 8-

10-3000 <5% CV @

tube/6-well

Precision Accuracy

<10% CV
@ 1
µL/well
(typical

+/-10%
(typical
+/-3%) @
1 µL/well

<3% CV)

<10% CV
@ 1
µL/well
(typical

+/-10%
(typical
+/-3%) @
1 µL/well

<3% CV)

±2 µL @

20 µL/well

10
µL/well

Approximate Dead
volume

Cassette Type:

1 µL 1.20 mL

5 µL 4.23 mL

10 µL 7.36 mL

Type Dead Vol.

1 µL 150 µL

5 µL 530 µL

10 µL 920 µL

For all manifold types:
12 mL

Syringe 16-tube 5-3000 <10% CV

@ 5
µL/well

±2 µL @
10
µL/well

Syringe 12-/24- 5-30,000 <3% CV @ ±2 µL @

BioTek Instruments, Inc.

8 | Chapter 1: Introduction

Volume

Device

/48-well certain

Syringe 32-tube 3-3000 <12% CV

Strip Washer 20 –

*1 µL cassettes' maximum recommended dispense volume is 50 µL/well.
‡ 0.5 µL dispensing is supported when using a 1 µL cassette.

¥Depending on wash manifold, See Performance Specifications on page 21.

range
µL/well

30,000

Precision Accuracy

any

µL/well

@ 6
µL/well

≤3.0 - 5.0

¥

% CV

µL/well

±5% @ 6
µL/well

≤±3.0% 12 mL

Approximate Dead
volume

BioTek recommends priming a dispenser with three times its dead volume to
prepare it for accurate dispensing.

Processing Time

§

Protocols were optimized for speed to obtain the following processing times,
including the fastest flow and travel rates. Some of these parameters are listed in
the Parameters column of the table. Only standard, non-RAD Peri-pump
cassettes are referenced here.

Device Plate

Type

Peri-pump - 5 µL 96 10 High flow rate 3
Peri-pump - 1 µL 384 1 High flow rate 6

1536 1 High flow rate 21
Syringe 8-tube 96 20 Flow rate 1 6.5
Syringe 16-tube 96 20 Flow rate 1 5.25

384 20 Flow rate 1 14
Syringe 32-tube 1536 3 One SB manifold 16.5

1536 14 One LB manifold 27
Strip Washer 96 300 3 cycles <105

Volume
(µL/well)

Parameters Time in

seconds

§ Review the Specifications for more details.

¥ Excluding plate carrier and manifold homing movements.

SB = small bore Syringe manifold; LB = large bore manifold.

¥

MultiFlo™ FX Multi-Mode Dispenser