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Operator’s Manual
Dispenser
MicroFlo
™
Select
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MicroFlo Select™
Microplate Dispenser
Operator’s Manual
February 2010
© 2010
Part Number 7171000
Revision C
BioTek® Instruments, Inc.
Page 4
ii | MicroFlo Select™
Notices
BioTek® Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
All Rights Reserved
© 2010, BioTek® Instruments, Inc. All rights are reserved. No part of this manual may
be reproduced or transmitted in any form, or by any means electronic or mechanical,
including photocopying and recording for any purpose other than the purchaser’s
personal use without written permission of BioTek
Trademarks
BioTek is a registered trademark, and MicroFlo™ Select, BioStack™ , and Liquid
Handling Control™ Software are trademarks of BioTek Instruments, Inc.
®
Instruments, Inc.
Microsoft
®
, Windows® , Windows XP, Windows 2000, Windows Vista™, and Excel®
are either registered trademarks or trademarks of Microsoft Corporation in the United
States and/or other countries.
All other trademarks are the property of their respective holders.
Restrictions and Liabilities
Information in this document is subject to change and does not represent a
commitment by BioTek. Changes made to the information in this document will be
incorporated in new editions of the publication. No responsibility is assumed by
BioTek for the use or reliability of software or equipment that is not supplied by
BioTek or its affiliated dealers.
BioTek Instruments, Inc.
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Contents
Notices ......................................................................................ii
Contents................................................................................... iii
Contacting BioTek Instruments, Inc. ............................................ vii
Revision History....................................................................... viii
Document Conventions ............................................................. viii
Intended Use Statement .............................................................ix
Quality Control...........................................................................ix
Warranty & Product Registration....................................................x
Cassette Warranty.......................................................................x
Warnings ...................................................................................x
Hazards .....................................................................................x
Precautions ...............................................................................xi
CE Mark................................................................................... xii
Electromagnetic Interference and Susceptibility............................ xiv
User Safety ............................................................................. xiv
Safety Symbols......................................................................... xv
Introduction..............................................................................1
The MicroFlo Select Microplate Dispenser....................................... 2
Hardware Features..................................................................... 4
Package Contents....................................................................... 5
Optional Accessories................................................................... 6
Specifications ............................................................................ 7
Contents | iii
All Rights Reserved.................................................................ii
Trademarks...........................................................................ii
Restrictions and Liabilities........................................................ii
Customer Service and Sales................................................... vii
Service/TAC ........................................................................ vii
European Coordination Center/ Authorized European
Representative..................................................................... vii
Regulatory Agency Approvals..................................................ix
Directive 2004/108/EC: Electromagnetic Compatibility.............. xiii
Directive 73/23/EEC: Low Voltage (Safety) ............................. xiii
Directive 2002/96/EC: Waste Electrical and Electronic Equipmentxiii
Directive 2002/95/EC: Reduction of Hazardous Substances (RoHS)xiv
Directive 98/79/EC: In Vitro Diagnostics................................. xiv
USA FCC CLASS A ............................................................... xiv
Canadian Department of Communications Class A.................... xiv
Software.............................................................................. 3
Compatibility with the BioStack Microplate Stacker..................... 3
MicroFlo Select Operator's Manual
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iv | MicroFlo Select™
Installation ...............................................................................9
1: Unpack and Inspect the Instrument .........................................10
Accessories Checklist ............................................................10
2. Remove the shipping hardware................................................12
3: Install the MicroFlo’s Components............................................14
Install the Tubing Cassette ....................................................14
Tubing Cassette Diagram.......................................................15
Insert the Prime Trough Drain Tube ........................................18
Prime Trough Insert..............................................................19
Power Supply and Cords........................................................20
Data Communication ............................................................20
Operating the MicroFlo with the BioStack.................................20
4: Verify Performance................................................................21
Repacking and shipping the MicroFlo............................................22
Before shipping the instrument: .............................................22
Repackaging the instrument and its accessories: ......................22
Repacking Illustration ...........................................................25
Essential Concepts..................................................................27
How it works ............................................................................28
How to optimize performance......................................................29
Quick Dispense or Protocol?........................................................31
Handling Special Plates and Test tube racks..................................33
6-, 12-, 24-, and 48-Well Plates .............................................33
Test Tube Racks...................................................................34
Chemical Compatibility...............................................................35
Table 1. Material/Where Used List...........................................35
Table 2. Chemical Compatibility Ratings...................................36
Operation................................................................................37
Basic Operation.........................................................................38
Turn On/Off the MicroFlo.......................................................38
Using the Keypad.................................................................38
Main Menu ..........................................................................39
Dispenser Run Menu.............................................................40
Dispenser Action Menu..........................................................40
Run a Quick Dispense ................................................................42
About Dispense Protocols ...........................................................43
Run a Dispense Protocol.............................................................47
Prime the MicroFlo.....................................................................48
Purge the MicroFlo.....................................................................49
Define the Cassette ...................................................................50
Removing the Tubing Cassette....................................................50
Define the Plate Type and Plate Map ............................................51
BioTek Instruments, Inc.
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Contents | v
About Plate Types and Plate Maps...........................................51
Setup Menu Options ..................................................................53
Utility Menu Options ..................................................................54
Using the BioStack ....................................................................55
Preventive Maintenance..........................................................57
Overview .................................................................................58
Recommended Maintenance Schedule..........................................58
Flush the Tubing Cassette...........................................................59
Record the number of plates processed ........................................60
Clean Exterior Surfaces..............................................................61
Clean Priming Trough and Drain Hose ..........................................61
Verify Performance....................................................................62
Unclogging the Dispense Tips......................................................63
Replace the Tubing....................................................................65
Recalibrate the Cassette.............................................................65
Decontamination.......................................................................66
Purpose ..............................................................................66
Tools and Supplies................................................................67
Decontamination Procedure ...................................................67
Instrument Qualification.........................................................69
IQ/OQ/PQ ................................................................................70
Recommended Qualification Schedule ..........................................71
System Self-Check and Checksum Test ........................................72
System Self-Check ...............................................................72
Checksum Test ....................................................................72
Dispense Precision and Accuracy Tests.........................................73
Materials.............................................................................75
Test Solutions......................................................................76
Performing the Precision and Accuracy Tests.................................77
Procedure: ..........................................................................77
Documenting Test Results .....................................................79
MicroFlo Select Dispense Precision & Accuracy Test Worksheet 1 µL
Cassette Test.......................................................................80
MicroFlo Select Dispense Precision & Accuracy Test Worksheet 5 µL
Cassette Test.......................................................................81
MicroFlo Select Dispense Precision & Accuracy Test Worksheet 1 µL
Cassette Test.......................................................................82
MicroFlo Select Dispense Precision & Accuracy Test Worksheet 10
µL Cassette Test ..................................................................83
MicroFlo Select Dispense Precision & Accuracy Test Worksheet 5 µL
Cassette Test.......................................................................84
MicroFlo Select Dispense Precision & Accuracy Test Worksheet 10
µL Cassette Test ..................................................................85
MicroFlo Select Operator's Manual
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vi | MicroFlo Select™
MicroFlo Select Dispense Precision & Accuracy Test Worksheet
1536 Cassette Test...............................................................86
Troubleshooting......................................................................87
Performance Issues ...................................................................88
Error Codes..............................................................................89
Contact BioTek Technical Assistance Center ..................................90
Service/Technical Assistance Center (TAC)...............................90
European Coordination Center/ Authorized European
Representative.....................................................................90
Error Codes.............................................................................91
Error Codes..............................................................................92
Instrument Error Codes.........................................................92
Software Error Codes............................................................94
Dispensing to Test Tubes ........................................................95
Install the test tube rack ............................................................96
Dispensing to the test tube rack..................................................97
Gravimetric Calibration Instructions.......................................99
Calibrating the Tubing Cassette Gravimetrically........................... 100
Procedure: ........................................................................ 101
Gravimetric Cassette Calibration Worksheet...........................104
About Calibrating Cassettes Gravimetrically ................................105
Index ....................................................................................109
BioTek Instruments, Inc.
Page 9
Contacting BioTek Instruments, Inc. | vii
Contacting BioTek Instruments, Inc.
BioTek Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
Customer Service and Sales
Internet: www.biotek.com
Phone: 888-451-5171 (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: 802-655-7941
E-Mail: customercare@biotek.com
Service/TAC
Phone: 800-242-4685 (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: 802-654-0638
E-Mail: tac@biotek.com
European Coordination Center/
Authorized European Representative
BioTek Instruments GmbH
Kocherwaldstrasse 34
D-74177 Bad Friedrichshall
Germany
Internet: www.biotek.de
Phone: +49 (0) 7136 9680
Fax: +49 (0) 7136 968 111
E-Mail: info@biotek.de
MicroFlo Select Operator's Manual
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viii | MicroFlo Select™
Revision History
Rev Date Changes
A 10/2007 First Issue
B 08/2008
C 02/2010
Revised the repackaging artwork and instructions in the
Installation chapter to reflect new packaging materials.
Removed the hyphen from BioStack. Updated the Trademarks
section by adding the BioStack, Liquid Handling Control™
Software, Microsoft, and Excel. Added a Cassette Warranty to
the Preface. Updated the Registration section in the Preface
by adding an instruction to review the Warranty for the
instrument and register online through the Customer
Resource Center. Updated the TAC fax number and added
“ATTN: RMA # xxxxx” to the BioTek shipping address (for
returning the instrument). Added a part number disclaimer in
the Package Contents and Optional Accessories sections.
Description and pictures of the Dispense Cassette were
revised to match new design. Added descriptions of the Tip
Guard and break-away tabs. Added description of the new
Plate Clearance Height parameter.
Optional accessories were updated with angled dispensing and
tubing extensions products.
Revised the Intended Use statement for IVD.
Document Conventions
The intent of this operator’s manual is to instruct the new user how to set up and operate
BioTek’s MicroFlo Select Microplate Dispenser. To help you read and understand this
manual, certain document conventions have been used.
This manual uses the following typographic conventions:
Example Description
This icon calls attention to important safety notes.
Warning!
Caution
A Warning indicates the potential for bodily harm and
tells you how to avoid the problem.
A Caution indicates potential damage to the
instrument and tells you how to avoid the problem.
BioTek Instruments, Inc.
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Example Description
Intended Use Statement | ix
The menu path to open the feature described is shown in this format.
When applicable, the above format shows you how to navigate to the feature described
in that section.
Note: Information of particular importance or usefulness is presented
in this format.
Tips or other helpful information is presented in this format.
This icon calls attention to important informat ion.
Intended Use Statement
BioTek’s MicroFlo Select™ Microplate Dispenser accommodates all standard microplates
and deep well blocks for bulk reagent dispensing applications. The dispense tubing
cassettes are autoclavable for those applications requiring a sterile fluid path.
The MicroFlo Select™ can operate with standard robotic systems, such as BioTek’s
BioStack™ Microplate Stacker.
This product may be used for In Vitro Diagnostic, research and development, or other
non-clinical purposes.
Quality Control
It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert or
standard laboratory protocol for the test to be conducted. Failure to conduct Quality
Control checks could result in erroneous test data.
Regulatory Agency Approvals
The MicroFlo Select Microplate Dispenser has been tested, approved, and labeled to
show compliance to the test standards indicated on later pages in this section.
MicroFlo Select Operator's Manual
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x | MicroFlo Select™
Warranty & Product Registration
Please take a moment to review the Warranty information that shipped with your
product. Please also
important information and updates about the product(s) you have purchased.
You can register online through BioTek’s Customer Resource Center (CRC) at
www.biotek.com or by calling 888-451-5171 or 802-655-4740.
register your product with BioTek to ensure that you receive
Cassette Warranty
MicroFlo Select dispense cassettes are backed by BioTek's Confidence-Plus lifetime
warranty. For the lifetime of the cassette, BioTek guarantees the cassette components
(Tip Holder, Center Holder, Tube Tensioner, and Tube Organizer) will withstand
steam autoclave conditions of 121°C and 1 bar (750 mmHg) without adversely affecting
dispense performance. In the event of a failure of any cassette component previously
listed, BioTek will replace the cassette components free of charge.
Warnings
Operate the instrument on a flat surface away from excessive humidity.
When operated in a safe environment according to the instructions in this
document, there are no known hazards associated with the instrument. However,
the operator should be aware of certain situations that could result in serious
injury; these vary depending on the instrument type. See Hazards and
Precautions.
Hazards
The following hazards are provided to help avoid injury:
Warning! Power Rating.
be connected to a power receptacle that provides voltage and current within the
specified rating for the system. Use of an incompatible power receptacle may
produce electrical shock and fire hazards.
Warning! Electrical Grounding.
connect primary power to the external power supply. Use of a two-prong adapter
disconnects the utility ground, creating a severe shock hazard. Always connect the
power cord directly to an appropriate receptacle with a functional ground.
The instrument’s power supply or power cord must
Never use a two-prong plug adapter to
BioTek Instruments, Inc.
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