Bio-Tek FL600 Hardware Manual

BIO-TEK FL600

FLUORESCENCE

PLATE READER

HARDWARE GUIDE

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BIO-TEK FL600

FLUORESCENCE

PLATE READER

For Research & Development Use Only

HARDWARE GUIDE

™

Part No. 6001000

June, 1998
Revision D

Notices

BIO-TEK INSTRUMENTS, INC.
Highland Park, Box 998
Winooski, Vermont, USA
05404-0998

Customer Service & Sales: 888-451-5171
Outside U.S.: 802-655-4040
Service: 800-24-BIOTK
Sales FAX: [phone]
Service FAX: [phone]
E-MAIL: [e-mail]
[e-mail]
Internet: http://www.biotek.com

Lionheart Technologies, Europe N.V.
Sneeuwbeslaan 33
B-2610 Wilrijk
Belgium
TEL: [phone]
FAX: [phone]

Copyright

 Copyright 1997, Bio-Tek Instruments, Incorporated. No part of this
publication may be reproduced, transmitted, transcribed, stored in a retrieval
system, or translated into any language without the written permission of Bio-Tek
Instruments, Incorporated.

Trademarks

Bio-Tek® is a registered trademark, and FL600™ and KC4™ are trademarks of

Bio-Tek Instruments, Inc.. Windows® and Windows 95 are trademarks of the
Microsoft Corporation.

Restrictions and Liabilities

Information in this document is subject to change, and does not represent a
commitment by Bio-Tek Instruments, Inc. Changes made to the information in
this document will be incorporated in new editions of the publication. No
responsibility is assumed by Bio-Tek for the use or reliability of software or
equipment that is not supplied by Bio-Tek, or its affiliated dealers.

Bio-Tek Part No. 6001000

Bio-Tek FL600 iii

Revision History

Revision Date Changes

A 4/97 Release to production

B 10/97 Added Stay Clear safety symbol. Corrected Electrical, Optical,

and Performance Specifications sections. Added CE Mark
information. Deleted Appendix A.

C3/98

D6/98

Preface: Added warranty. Page 2-1: Modified instructions for
Setting Up the FL600 Reader. Page 5-2: Changed electrical
specification for fuses. Page 5-11: Modified text and data on
serial communications to reflect parity differences between KC4
reader software and FL600 diagnostic software. Page 5-13: On
filter table, modified use of excitation filters and added two new
filter wavelengths.

Preface: Changed phone numbers and e-mail addresses.
Chapter 4: Changed air filter maintenance procedure. Added

lamp replacement procedure.

iv Bio-Tek FL600

Warranty

This Warranty is limited and applies only to new products, except for computer-based
software which is covered under a separate Warranty Policy, manufactured by
Bio-Tek® Instruments, Inc. (“Bio-Tek”). Bio-Tek makes no warranty whatsoever
regarding the condition of used products.

Bio-Tek warrants the instrument (hereinafter collectively referred to as “Products” or
“Product”) for a period of one (1) year from the original purchase date against defective
materials or workmanship. This Warranty is limited to the original purchaser (the
“Purchaser”) and cannot be assigned or transferred. All claims under this Limited
Warranty must be made in writing to Bio-Tek, Attention: Service Department.
Purchaser must ship the Product to Bio-Tek, postage pre-paid. Bio-Tek shall either
repair or replace with new or like-new, at its option and without cost to the Purchaser,
any Product which in Bio-Tek’s sole judgment is defective by reason of defects in the
materials or workmanship.

This Warranty is VOID if the Product has been damaged by accident or misuse, or has
been damaged by abuse or negligence in the operation or maintenance of the Product,
including without limitation unsafe operation, operation by untrained personnel, and
failure to perform routine maintenance. This Warranty is VOID if the Product has been
repaired or altered by persons not authorized by Bio-Tek, or if the Product has had the
serial number altered, effaced, or removed. This Warranty is VOID if any of the
Products has not been connected, installed or adjusted strictly in accordance with
written directions furnished by Bio-Tek. Batteries, fuses, lightbulbs, and other
“consumable” items used in any of the Products are not covered by this Warranty.
Software utilized in conjunction with any of the Products is not covered by the terms of
this Warranty but may be covered under a separate Bio-Tek software warranty.

We will continue to stock parts for a maximum period of five (5) years after the
manufacturer of any equipment has been discontinued. Parts shall include all materials,
charts, instructions, diagrams, and accessories that were furnished with the standard
models.

THIS WARRANTY CONTAINS THE ENTIRE OBLIGATION OF BIO-TEK
INSTRUMENTS, INC., AND NO OTHER WARRANTIES, EXPRESSED, IMPLIED,
OR STATUTORY ARE GIVEN. PURCHASER AGREES TO ASSUME ALL
LIABILITY FOR ANY DAMAGES AND/OR BODILY INJURY OR DEATH
WHICH MAY RESULT FROM THE USE OR MISUSE OF ANY EQUIPMENT OR
INSTRUMENT BY THE PURCHASER, HIS EMPLOYEES, AGENTS, OR
CUSTOMERS, OTHER THAN THE EXPRESS WARRANTY CONTAINED
HEREIN. WE SHALL NOT BE RESPONSIBLE FOR ANY DIRECT OR
CONSEQUENTIAL DAMAGES OF ANY KIND. THIS WARRANTY SHALL NOT
BE CHANGED OR MODIFIED IN ANY WAY WITHOUT THE EXPRESS
WRITTEN PERMISSION OF AN OFFICER OF BIO-TEK INSTRUMENTS, INC.

Bio-Tek FL600 v

Warnings and Precautions

When operated in a safe environment, according to the instructions in this manual,
there are no known hazards associated with the instrument. However, the
operator should be aware of certain situations which could result in serious injury.

Hazard Warnings

Warning! Power Rating. The instrument must be connected to a power
receptacle that provides voltage and current within the specified rating for the
system. Use of an incompatible power receptacle may produce electrical shock
and fire hazards.

Warning! Electrical Grounding. Never use a two-prong plug adapter to
connect primary power to the instrument. Use of a two-prong adapter disconnects
the utility ground, creating a severe shock hazard. Always connect the system
power cord directly to a three-prong receptacle with a functional ground.

Warning! Internal Voltage. Always turn off the power switch and unplug the
power cord before cleaning the outer surfaces of the instrument or changing
filters.

Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into
internal components creates a potential shock hazard. Wipe up all spills
immediately. Do not operate the instrument if internal components have been
exposed to fluid. If the microplate appears to have been exposed to fluid, the
reading chamber may also have been exposed. To protect the incubator, remove
the top cover and clean the interior.

Precautions

The following precautions are provided to help you avoid damaging the system:

Caution: Service. The system should be serviced by Bio-Tek-authorized service
personnel. Only qualified technical personnel should perform troubleshooting and
service procedures on internal components.

Caution: Replacement Fuses. Use replacement fuses with the required current
rating and specification. Improper fuses or short-circuiting the fuse holders may
cause fire or damage the instrument.

vi Bio-Tek FL600

Caution: Environmental Conditions. Do not expose the system to temperature
extremes. System performance may be adversely affected.

Caution: Filter Settings. The software filter settings must match the actual
filters used in the reader, in order to prevent possible damage to the
photomultiplier and/or degradation of data.

Safety Symbols

The following warning and informational symbols may be found in various
locations on the instrument. Only qualified personnel who recognize shock
hazards and are familiar with the safety precautions should use this instrument.
Read the manual carefully before operating this instrument.

Direct current

Courant continue
Gleichstrom
Corriente continua
Corrente continua

Alternating current

Courant alternatif
Dreiphasen-Wechselstrom
Corriente Atterna
Corrente alternata

Both direct and alternating current

Courant continu et courant alternatif
Allstrom (Gleich - und Wechselstrom
Corriente continua y corriente alterna
Corrente continua e corrente alternata

Earth ground terminal

Borne de terre
Erde (Bettriebserde)
Borne de Tierra
Terra (di funzionamento)

Protective conductor terminal

Borne de terre de protection
Schutzleiteranscluss
Borne de Tierra de Protección
Terra di protezione

On (Supply)

Marche (alimentation)
Ein (Verbindung mit dem Netz)
Connectado
Chiuso

Bio-Tek FL600 vii

Off (Supply)
Arrest (alimentation)
Aus (Trennung vom Netz)
Desconectado
Aperto (sconnessione dalla rete di alimentazione)

Caution (Refer to accompanying documents)

Attention (voir documents d'accompanement)
Achtung siehe Begleitpapiere
Atención (vease los documentos incluidos)
Attenzione, consultare la doc annessa

Caution, risk of electric shock

Attention, risque de choc electrique
Gefährliche elektrische Spannung
Atención, riesgo de sacudida eléctrica
Alta tensione (in questo documento
Alta tensione non significa "te nsione pericolosa"
come definito in IEC 417)

Stay clear

Ne pas toucher svp .
Bitte nicht berühren
No tocario por favor
Si prega di non toccare

viii Bio-Tek FL600

Contents

1. Introducing the Bio-Tek FL600

Hardware Requirements 1-3
Software Requirements 1-4

2. Getting Started

Setting Up the FL600 Reader 2-1
Installing KC4™ and FL600 Diagnostic Software 2-2

Installing KC4 2-2
Installing the Hardware Key 2-2
Installing the FL600 Diagnostic Software (Diagnostics Use)2-2

Checking the FL600 System 2-4

Using the FL600 Diagnostic Software 2-4
Filter Wavelength Locations 2-5
Checking the COM Port 2-5

Running System Diagnostics 2-7

What to Do if the Tests Fail 2-8

Exiting the FL600 Diagnostic Software 2-9

3. Replacing Filters and Adjusting Probe Height

Adding and Replacing Filters 3-1

Changing the Filters Inside the Reader 3-2
Setting the Filter Positions in KC4 Software 3-6

Utilizing the Filter Adapter System 3-7

Removing the Filter from the Adapter 3-7

Inserting the Replacement Filter into the Adapter 3-8
Adjusting Top Optical Probe Height 3-8
FL600 Interior Configuration Diagrams 3-10

4. Bio-Tek FL600 Maintenance

Maintaining the Reader 4-1

How to Maintain the Exterior 4-1

How to Replace the Air Filter 4-1

How to Replace the Lamp 4-3

Bio-Tek FL600 ix

5. Technical Information

Bio-Tek FL600 Fluorescence/Absorbance Plate Reader 5-1

Physical Dimensions 5-1

Operating Conditions 5-1

Electrical Specifications 5-2

Optical Specifications 5-2

Scanning Mechanism 5-3

Temperature Control 5-4

Performance Specifications 5-4

CE Mark Approval 5-8
Computer Specifications for FL600 Diagnostic Software 5-10
FL600 Serial Communications 5-11
Ordering Filters 5-12

Fluorescence Filters 5-12

Absorbance Filters 5-14

x Bio-Tek FL600

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This chapter introduces the Bio-Tek FL600 Fluorescence

Plate Reader. You'll find information on hardware and

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Thank you for selecting the Bio-Tek FL600. The FL600 is a powerful

fluorescence and absorbance measurement system which measures

through the use of a photomultiplier tube housed inside of a light-

impermeable detection compartment. The FL600 is capable of quantifying

both soluble and cell-associated fluorescence. The system consists of:

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software requirements and optional equipment

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suggestions.

• Accessories Kit

Both software packages are for Windows-based PCs and provides the user

with full command of the operation of the X-Y scanner. FL600’s precision

X-Y mechanism scans a microplate with a sensitive optical detection

system to quantify the fluorescence or absorbance in each well.

• The Bio-Tek FL600 Fluorescence Plate Reader

• KC4™ reader control and data reduction software

• FL600 System Software for diagnostics

• Serial Communications Cable (P/N 75034)

Virtually any microplate type from 6 to 384 wells can be used with the

FL600. Users can select any microplate from the FL600’s built-in list of

industry-standard plates, or they can define their own unique plate

format(s).

The FL600 is designed to meet international radio frequency emission

standards, EMC immunity standards, and user safety standards.

The Bio-Tek FL600 should be installed in a well-ventilated area, free from

dust and fumes. Keep the system away from direct sunlight and local heat

sources.

1-2

Introduction to the Bio-Tek FL600

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• IBM™ 486 PC or clone 33 MHz clock

• 640 KB base memory, extended to 8 MB

• Color graphics monitor (VGA)

• Color graphics adapter (VGA)

• 120 MB hard drive

• High-density floppy disk drive 3½"

• 1 serial (COM) and 1 parallel (LPT) port

• Mouse and keyboard ports

• Microsoft or compatible mouse

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• IBM 486 PC or clone 100 MHz clock

• 640 KB base memory, extended to 8 MB

• Color graphics monitor (VGA)

• Color graphics adapter (VGA)

• 120 MB hard drive

• High-density floppy disk drive 3½"

• 1 serial (COM) and 1 parallel (LPT) port

• Mouse and keyboard ports

• Microsoft or compatible mouse

• Surge/spike protector

Introduction to the Bio-Tek FL600

1-3

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• IBM 486 / 100 MHz PC or better compatible

• Memory: 640 KB base, extended to 16 MB minimum

• 250 MB hard drive

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• Microsoft Windows compatible printer

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• FL600 System Software, version 2 or higher

• KC4™ reader control and data reduction software

• MS-DOS version 5.0 or later

• Microsoft Windows version 3.1 or later, or Microsoft

Windows 95 (with 16 MB memory)

1-4

Introduction to the Bio-Tek FL600

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This chapter discusses how to set up the Bio-Tek FL600

hardware, check the communication port and run the

diagnostic tests, install and configure the FL600 software,

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and exit the FL600 system software.

CAUTION: It is recommended that two people lift the FL600, which weighs about 23

kg (50.6 lb.). Care must be taken when moving the device or removing it from the

packaging. Hold the device firmly from the bottom of the case when lifting it.

Setting up the Bio-Tek FL600 Fluorescence Plate Reader is simply a matter of
connecting the system to the computer via the computer’s serial port, installing
the software, and setting up communications parameters. One of the serial ports
most likely already has a mouse connected to it (the FL600 software, since it
operates under Windows, requires a mouse).

Step 1:

Step 2:
Step 3:

Step 4:

Step 5:

110V for 100-120V˜ operation,
220V for 200-240V˜ operation

Set the incubator power switch to the appropriate voltage as
follows:

Turn off the PC.
To connect the FL600, first attach the 9-pin D-type cable into the

computer’s serial port (COM1, COM2, COM3, or COM4).
Plug the other end of the cable into the Serial Data Port located at

the rear of the FL600 Fluorescence Plate Reader.
Connect the power cable to the back of the Plate Reader.

Step 6:

Plug all power cords into grounded, electrical outlets (use of a surge
protector is strongly recommended).

The FL600 and all computer hardware must be electrically grounded and

connected to a viable surge protector to avoid dangerous shocks and to

avoid destroying the electronic components through electrical spikes and

surges.

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The FL600 is supplied with two Windows™ software packages, KC4™

and FL600 Diagnostic Software.

KC4 controls the reader, reduces the fluorescence and absorbance data,

and furnishes the reports.

The FL600 Diagnostic software provides diagnostics to test the reader.

Although it contains other functions, these are undocumented and

unsupported.

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Please consult your KC4™ User’s Guide. Place disk 1 into the floppy

drive. Run Setup.exe by choosing the Run option from the File Menu of

your Windows program. This routine will guide you through the

installation of KC4 onto your hard disk.

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To use KC4 to control the reader and print data, the hardware key needs to

be installed on the parallel port of your computer. Please consult your

KC4 Operations manual for further instruction.

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To install the FL600 software, follow these steps:

Step 1:

Step 2:
Step 3:
Step 4:

Turn on your computer, start Windows, and activate Windows’
Program Manager, if necessary.

Insert the FL600 Setup diskette into the floppy drive.
Select

Run

from the Windows’

File

menu.

Type drive: setup, (i.e., b: setup).

2-2

Getting Started

Setup will indicate to you that all FL600 diagnostic software files will be

installed into the drive and directory C:\FL600. You will be given the

opportunity to change either the drive or the directory (or both). Setup

creates a new group called Bio-Tek FL600. Note that this is different from

the KC4™ group created by the KC4™ software.

NOTE: Be sure that TrueType Fonts are enabled in the Fonts Section of the

Windows Control Panel. FL600 requires certain TrueType fonts when
printing reports. For more information on activating TrueType Fonts,
please check your Windows User’s Guide.

Getting Started

2-3