Biotek Epoch 2 Instructions For Use Manual

Epoch 2

Microplate Spectrophotometer

INSTRUCTIONS FOR USE

BioTek Instruments, Inc.

1771011 Revision A

Preface Page 2 of 42

Contents

Installation ............................................................................................................ 12

Getting Started ..................................................................................................... 17

Maintenance ........................................................................................................ 33

Qualification ......................................................

Specifications........................................................................................................ 43

....................................................39

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Preface Page 3 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Preface

Notices

BioTek® Instruments, Inc.

100 Tigan Street

Highland Park, P.O. Box 998

Winooski, Vermont 05404-0998 USA

All Rights Reserved

© 2018, BioTek® Instruments, Incorporated. No part of this publication may be

reproduced, transcribed, or transmitted in any form, or by any means electronic or
mechanical, including photocopying and recording, for any purpose other than the

purchaser’s use without written permission of BioTek Instruments, Inc.

Trademarks

BioTek® is a registered trademark, and Epoch™, Take3™, BioStack™, and Gen5™ are

trademarks of BioTek Instruments, Inc. BioCell™ is a trademark of BioTek Instruments
and is patented under U.S. patent number 5,963,318.

Microsoft®, Windows®, and Excel® are either registered trademarks or trademarks of
Microsoft Corporation in the United States and/or other countries.

All other trademarks are the property of their respective holders.

Restrictions and Liabilities

Information in this document is subject to change and does not represent a
commitment by BioTek Instruments, Inc. Changes made to the information in this
document will be incorporated in new editions of the publication. No responsibility is
assumed by BioTek for the use or reliability of software or equipment that is not
supplied by BioTek or its affiliated dealers.

Preface Page 4 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Contact Information

Global Service and Support

Instrument service and repair is available worldwide

www.biotek.com/service_support

U.S. Headquarters

BioTek® Instruments, Inc.
100 Tigan Street
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
www.biotek.com

Customer Service/Sales: 802-655-4740 or [e-mail]
Technical Assistance Center (TAC): 802-655-4740 or [e-mail]

European Coordination Center/Authorized European Representative

BioTek® Instruments GmbH
Kocherwaldstrasse 34
D-74177 Bad Friedrichshall
Germany

www.biotek.de

Phone: [phone]

Fax: [phone]

Email: [e-mail]

Instructions for Use Requirements

This document fulfills the basic needs of persons operating this device, according to
the requirements of the In Vitro Diagnostic Directive (98/79/EC) for “Instructions for
Use.” Some of the device's higher-level functions and features, as well as certain
detailed maintenance and qualification routines, are described in the operator’s
manual.

Intended Use Statement

This instrument is intended for IVD use. The performance characteristics of the data
reduction software have not been established with any laboratory diagnostic assay.
Users must evaluate this instrument and PC-based software in conjunction with their
specific assay(s). This evaluation must include the confirmation that performance
characteristics for the specific assay(s) are met.

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Quality Control

It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for the
test to be conducted. Failure to conduct Quality Control checks could result in
erroneous test data.

Warnings

Operate the instrument on a level, stable surface away from excessive humidity.

Bright sunlight or strong incandescent light can reduce the linear performance
range of the instrument.

Measurement values may be affected by extraneous particles in the microplate
wells. A clean work area is necessary to ensure accurate readings.

When operated in a safe environment according to the instructions in this
document, there are no known hazards associated with the instrument.
However, the operator should be aware of certain situations that could result in
serious injury; these may vary depending on the instrument model. See Hazards
and Precautions.

Hazards

The following hazard warnings are provided to help avoid injury:

Warning! Internal Voltage. Always turn off the power switch and unplug the
power supply before cleaning the outer surface of the instrument.

Warning! Power Rating. The instrument’s power supply or power cord must
be connected to a power receptacle that provides voltage and current within
the specified rating for the system. Use of an incompatible power receptacle
may produce electrical shock and fire hazards.

Warning! Electrical Grounding. Never use a plug adapter to connect primary
power to the external power supply. Use of an adapter disconnects the utility
ground, creating a severe shock hazard. Always connect the power cord
directly to an appropriate receptacle with a functional ground.

Warning! Service. Only qualified technical personnel should perform service
procedures on internal components.

Warning! Accessories. Only accessories that meet the manufacturer's
specifications shall be used with the instrument.

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Warning! Lubricants. Do not apply lubricants to the microplate carrier or
carrier track. Lubricant on the carrier mechanism or components in the carrier
compartment will attract dust and other particles, which may obstruct the
carrier path and cause the instrument to produce an error.

Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into
internal components creates a potential for shock hazard. If a spill occurs while
a program is running, abort the program and turn off the instrument. Wipe up
all spills immediately. Do not operate the instrument if internal components
have been exposed to fluid. Contact BioTek Technical Assistance Center for
assistance.

Warning! Unspecified Use. Failure to operate the equipment according to the
guidelines and safeguards specified in this manual could result in a hazardous
condition.

Warning! Software Quality Control. The operator must follow the

manufacturer’s assay package insert when modifying software parameters and

establishing reading methods. Failure to conduct quality control checks could
result in erroneous test data.

Warning! Reader Data Reduction Protocol. No limits are applied to the raw
measurement data. All information displayed on the screen, sent to an
attached printer, or exported via computer control must be thoroughly
analyzed by the operator.

Warning! Potential Biohazards. Some assays or specimens may pose a
biohazard. This hazard is noted by the symbol shown here. Adequate safety
precautions should be taken as outlined in the assay’s package insert. Always
wear safety glasses and appropriate protective equipment, such as chemical­resistant rubber gloves and apron.

Precautions

The following precautions are provided to help avoid damage to the instrument:

Caution: Service. The instrument should be serviced by BioTek-authorized
service personnel. Only qualified technical personnel should perform service
procedures on internal components.

Caution: Spare Parts. Only approved spare parts should be used for
maintenance. The use of unapproved spare parts and accessories may result in
a loss of warranty and potentially impair instrument performance or cause
damage to the instrument.

Caution: Environmental Conditions. Do not expose the system to temperature
extremes. For proper operation, the temperature near the instrument should
remain within the range listed in Specifications. Performance may be
adversely affected if temperatures fluctuate above or below this range.

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Caution: Sodium Hypochlorite. Do not expose any part of the instrument to
the recommended diluted sodium hypochlorite solution for more than 20
minutes. Prolonged contact may damage the instrument surfaces. Be certain
to rinse and thoroughly wipe all surfaces.

Caution: Power Supply. Use only the power supply shipped with the
instrument. Operate this power supply within the range of line voltages listed
on it.

Caution: Shipping Hardware. The shipping hardware must be removed before
operating the instrument and reinstalled before repackaging the instrument
for shipment.

Caution: Disposal. Dispose of the instrument according to Directive
2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or local
ordinances.

Caution: Warranty. Failure to follow maintenance protocols may void the
warranty. See Maintenance.

Caution: Electromagnetic Environment. Per IEC 61326-2-6 it is the user’s
responsibility to ensure that a compatible electromagnetic environment for
this instrument is provided and maintained in order that the device will
perform as intended.

Caution: Electromagnetic Compatibility. Do not use this device in close
proximity to sources of strong electromagnetic radiation (e.g., unshielded
intentional RF sources), because these may interfere with the proper
operation.

Caution: Touchscreen. Do not use sharp implements to operate the
touchscreen.

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CE Mark

Directive 2014/30/EU: Electromagnetic Compatibility

Emissions—Class A

The system has been type-tested by an independent, accredited testing laboratory and
found to meet the requirements of EN 61326-1: Class A for Radiated Emissions and
Line Conducted Emissions.

Verification of compliance was conducted to the limits and methods of EN 55011 –
(CISPR 11) Class A. In a domestic environment it may cause radio interference, in which
case you may need to mitigate the interference.

Immunity

The system has been type-tested by an independent, accredited testing laboratory and
found to meet the requirements of EN 61326-1 and EN 61326-2-6 for Immunity.
Verification of compliance was conducted to the limits and methods of the following:

EN 61000-4-2, Electrostatic Discharge
EN 61000-4-3, Radiated Radio Frequency (RF) Immunity
EN 61000-4-4, Electrical Fast Transient/Burst Immunity
EN 61000-4-5, Surge Immunity
EN 61000-4-6, Conducted RF Disturbance Immunity
EN 61000-4-11, Voltage Dips, Interruptions and Short Variations

Directive 2014/35/EU Low Voltage (Safety)

The system has been type-tested by an independent testing laboratory and was found
to meet the requirements of this Directive. Verification of compliance was conducted
to the limits and methods of the following:

EN 61010-1. "Safety requirement for electrical equipment for measurement, control

and laboratory use. Part 1, General requirements."

EN 61010-2-010. "Particular requirements for laboratory equipment for the heating of

materials."

Directive 2012/19/EU: Waste Electrical and Electronic Equipment

Disposal Notice: Dispose of the instrument according to Directive 2012/19/EU, “on

waste electrical and electronic equipment (WEEE)” or local ordinances.

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Electromagnetic Interference and Susceptibility

Canadian Department of Communications Class A: This digital apparatus does not

exceed Class A limits for radio emissions from digital apparatus set out in the Radio
Interference Regulations of the Canada Department of Communications.

User Safety

This device has been type-tested by an independent laboratory and found to meet the
requirements of the following:

• Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety

requirements for electrical equipment for measurement, control and laboratory
use; Part 1: General requirements.”

• EN 61010 Standards, see CE Mark starting on page 8.

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Safety Symbols

Some of these symbols appear on the instrument or accessories:

Alternating current

Both direct and alternating current

Direct current

Earth ground terminal

Protective conductor terminal
On (Supply)

Off (Supply)

Caution (refer to accompanying documents)

Warning, risk of electric shock
Warning, risk of crushing or pinching

Warning, potential biohazards

Warning, hot surface

Laser radiation: Do not stare into beam

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Laser energy: Exposure near aperture may cause burns. Do not
stare directly at the laser during operation.

In vitro diagnostic medical device

Separate collection for electrical and electronic equipment

Consult instructions for use

Installation Page 12 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Installation

Models

Model

Touchscreen

Cuvette Port

EPOCH2NS

EPOCH2NSC •

EPOCH2TS •

EPOCH2TSC • •

Package Contents

Item

Notes

A specific model of an Epoch 2 instrument
per sales order

All required accessories to power the
instrument

Interface cables, if required

User manual and/or instructions

Optional accessories per sales order,
unless shipped separately

Installation Page 13 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Unpack the Box

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Remove the Shipping Hardware

Do not turn on the reader before the shipping hardware is removed!

Select an Appropriate Location

Install the reader on a level, stable surface. For models without a touchscreen, select
an area where temperatures between 18°C and 40°C can be maintained. For models
with a touchscreen, the temperature must be between 18°C and 30°C. The reader is
sensitive to extreme environmental conditions. Avoid excessive humidity, excessive
ambient light, and dust.

(Gen5 control only) Prepare the Host Computer

❖ Follow instructions in the Gen5 Getting Started Guide to install the software.

(Gen5 control only) Connect the Host Computer and Reader

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Install the Power Supply

Install the Cuvette Holder

Models with a cuvette port

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Turn on the Reader and Run the Power-Up System Test

Allow the reader to settle at room temperature before you turn it on.

(Gen5 control only) Start Gen5 and Test Communication

1. Start Gen5. If prompted to add a reader, click Yes. Otherwise, select System >

Instrument Configuration > Add Reader.

2. Select Epoch 2 and click OK. The reader name and serial number appear. Click on the

reader name and then click Test Communications. The message, “The reader is

communicating!” should appear.

If the communication attempt is not successful: Make sure the reader is turned on and
the USB cable is secure on both ends. Consult the Gen5 Getting Started Guide
(supplied as a PDF on the Gen5 software USB flash drive) for additional
troubleshooting information.

Repackage the Instrument (if needed)

If the reader has been exposed to potentially hazardous material,
decontaminate it to minimize the risk to all who come in contact with the
reader during shipping, handling, and servicing. Decontamination prior to
shipping is required by the U.S Department of Transportation regulations.
See the Maintenance section for instructions.

Remove any labware from the carrier and, if used cuvette, before shipment.
Spilled fluids can contaminate the optics and damage the instrument.

Replace the shipping hardware before repackaging the reader.

Reverse unpacking instructions.

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Getting Started

External Components

Touchscreen Models

1. USB port for data

output

2. microplate carrier

access door

3. cuvette holder

(if equipped)

4. power on/off

5. carrier in/out,

status LED

6. USB port for printer

7. power inlet

8. USB port for host

computer
(if used)

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Non-Touchscreen Models

1. microplate carrier

access door

2. cuvette holder

(if equipped)

3. power on/off

4. carrier in/out,

status LED

5. power inlet

6. USB port for host

computer

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Optional Printer

Refer to the printer's manual for installation and setup instructions.

Using the Touchscreen

Use your fingertip to operate the touchscreen. A sharp stylus or pencil will damage
the surface. You can use a stylus designed for resistive touchscreens.

Main Menu

Up to 60 uniquely named protocols can be saved on the reader at one time, excluding
the predefined Take3 protocols.

Protocols created through the touchscreen are limited to a single read step and, for
Endpoint, Kinetic, and Take3 protocols, up to 12 blank wells. Use Gen5 if your assays
require more complex reading methods or plate layouts.

The left side of the Main Menu lists the five most recently used protocols. The tabbed
sections in the middle contain the protocols created for each protocol type, and the

Take3 plate (if used). To run an existing protocol, tap its name, define the wells to be

read if fewer than the full plate, and tap Start.

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The right side of the Main Menu provides access to the following features:

• Quick: Define and run an Endpoint read, perform a standalone shake, or

incubate a plate. Quick reads do not support shake or delay actions, and they
read the full plate.

• Protocol: Edit, create (and save), delete, and copy protocols. Through this menu

option, protocols support shake and delay actions.

• Results: Provides access to results for the 12 most recently run protocols. Tap a

protocol name to view its results, and then tap the screen to cycle through the
available data sets (e.g., Raw data, Delta OD, Blanked data).

• Instrument: Run a system test, define date/time settings, enable output to a

printer and/or USB flash drive, configure a Take3 plate, and control
temperature.

Confirm or Set the Time and Date

From the Main Menu, tap Instrument and select the Configuration tab.

• Tap the Time / Time Format and Date / Date Format fields to change the

current settings.

• Set the Decimal Symbol to a period or comma.

• Set the List Separator, used in exported .csv files, to a comma or semicolon.

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Define Output Formats for Results Data

From the Main Menu, tap Instrument and select the Output tab.

Output Types:

• To print results (Endpoint only), select Print or Save&Print.

• To save results to a USB flash drive, select Save to USB or Save&Print.

Data types:

• Raw: The raw measurement value for each well.

• Delta OD: Applicable when a secondary wavelength is selected in an

absorbance protocol. This is the calculated value for each well of the primary
wavelength measurement minus the secondary wavelength measurement.

• Blanked: The calculated value for each well, after subtracting the average of the

blank well(s).

Save Format options:

• Report: .csv file containing raw data, Delta OD, and/or blanked data, as

available. This file can be opened in Excel or other spreadsheet software.

• Gen5 Input: .txt file containing only raw data. This file can be opened in Gen5

using the Read from File option.

• Prompt: Choose an output format after the plate has been read.

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Define and Start a Quick Endpoint Read

From the Main Menu, tap Quick.

1. Select the Plate type and define the appropriate settings for your assay.

2. Place the plate on the carrier and tap Start to run the protocol.

3. When the read is finished, the results are displayed and ready for output according to

the settings defined under Instrument > Output.

Define and Start a Standalone Shake

From the Main Menu, tap Quick and select the Shake tab.

1. Set Shake Mode to Linear (side-to-side), Orbital (circle), or Double Orbital (figure 8).

2. Set the Duration, from 1 second to 2 hours 45 minutes.

3. For Orbital shake modes, set the Speed to Slow or Fast.

4. Adjust the Frequency, if needed. The cycles per minute updates as the slider moves.

The measurement in mm indicates the distance the carrier travels during the shake.

5. Place the plate on the carrier and tap Start to shake the plate.

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Incubate a Plate

From the Main Menu, tap Quick and select the Incubate tab.

1. Check the Active box.

2. Define a Set point from 18°C to 65°C (64.4°F to 149°F). Heating begins immediately.

3. Move the plate carrier in, if it is currently out.

4. When the temperature displayed at the top of the screen reaches the set point, move

the carrier out, insert the plate, and move the carrier back in.

The minimum set point must be ambient room temperature plus 4°C.

Condensation Control™
A temperature Gradient can be applied to reduce the risk of condensation when using

microplate lids. When no gradient (0) is applied, the heaters above and below the
plate operate at the same (setpoint) temperature. This is recommended if the plate
has no lid, or condensation is not a problem when lids are used.

When a gradient (0.1 to 2.0) is applied, the heaters above the plate operate slightly
higher than the set point and the heaters below the plate operate slightly lower than
the set point. The gradient represents the temperature separation between the top
and bottom heaters. This differential should serve to reduce or eliminate
condensation.

In the example below, the Set point is 37°C and the Gradient is 1.0:

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Some experimentation with the gradient setting may be necessary to achieve optimal
results with your assay.

Create and Save a Protocol

1. From the Main Menu, tap Protocol > Create.

2. Use the onscreen keyboard to enter a name for the protocol, then tap Save. Note that

the protocol name is limited to 18 characters.

3. Set the Read Mode to Endpoint, Kinetic, or Spectral.

4. Define the protocol parameters and then tap Save.

Endpoint Protocols

Plate: Options include standard 6-, 12-, 24-, 48-, 96-, and 384-well plates, Cuvette (if

equipped), and Take3 (if configured).

Primary WL and (optional) Secondary WL: Define the primary and (optional)

secondary wavelength in the range 200 to 999 nm.

Microplates and Take3 only:

Read Speed: Select Normal (optimum performance) or Sweep (optimum speed). See

Specifications to compare the performance and timing specifications.

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Blanks: Tap to open a plate matrix and select up to 12 Blank wells. For a 384-well

plate, the matrix is displayed in quadrants; tap the 1-2-3-4 box to change the view.

Activity Before Read options:

• Set a delay (up to 2 hours, 45 minutes) before the read.

• Select a shake mode of Linear (side-to-side), Orbital (circle), or Double Orbital

(figure 8). Set a Duration (up to 2 hours 45 minutes) to shake the plate before
the read begins. Adjust the Frequency, if needed. The cycles per minute updates
as the slider moves. The measurement in mm indicates the distance the carrier
travels during the shake.

When finished, tap Save. The protocol name is added to the Main Menu.

If your Endpoint assay requires incubation, activate Temperature Control before reading
the plate. From the Main Menu, select Instrument and tap the Temperature tab.

Kinetic Protocols

Time: Specify the full duration of the kinetic analysis, up to 272:15:00 (hh:mm:ss).

Note: You can stop a protocol while it is running, before the Time expires; results will
be saved.

Interval: Specify the interval (delay) between reads, up to 2:45:00 (hh:mm:ss). The

Interval must be shorter than the Time.

Number of kinetic reads: Calculated automatically, based on the Time and Interval

settings. Cannot exceed 100 reads.

Considerations: The Interval must be long enough to support the protocol parameters

and the number of microplate wells selected for reading. If a shake is specified, its
Duration may result in a longer interval than defined in the protocol. To test the

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settings, save the protocol and return to the Main Menu. Tap the protocol to run it,
select the wells if not reading the full plate, and tap Start. The Minimum Kinetic Interval
will display if the defined Interval is too short. Run the protocol using the displayed
interval, or return to the protocol and modify its parameters.

Plate: Options include standard 6-, 12-, 24-, 48-, 96-, and 384-well microplates,

Cuvette (if equipped), and Take3 (if configured).

Primary WL: Define a wavelength in the range 200-999 nm. Only one wavelength is

supported through the touchscreen; use Gen5 if your assay has other requirements.

Microplates and Take3 only:

Read Speed: Select Normal (optimum performance) or Sweep (optimum speed). See

Specifications to compare performance and timing specifications.

Blanks: Tap to open a plate matrix and select up to 12 Blank wells. For a 384-well

plate, the matrix is displayed in quadrants; tap the 1-2-3-4 box to change the view.

Options (tap Edit):

If applicable, check Eject the plate when done. If unchecked, the plate will remain in
the chamber after the last kinetic read.

If the assay requires incubation:

1. Check the Active box.

2. Define a Setpoint from 18°C to 65°C. Heating will begin when you start to run

the protocol.

3. Check Turn off when done to turn the incubator off after the last kinetic read is

completed.

The minimum setpoint must be ambient room temperature plus 4°C. See Specifications
for more information on temperature control.

Shake Before Each Read: Select a shake mode of Linear (side-to-side), Orbital (circle),

or Double Orbital (a figure 8). Set a Duration (up to 2 hours 45 minutes) to shake the
plate before each kinetic read or select Continuous to shake the plate whenever it is
not being read. Adjust the Frequency, if needed. The cycles per minute updates as the
slider moves. The measurement in mm indicates the distance the carrier travels during
the shake.

• Continuous shaking typically results in higher ODs and lower CVs.

• To define a kinetic protocol with a shake before the first read only, use Gen5.

When finished, tap Save. The protocol name is added to the Main Menu.

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Spectral Scan Protocols

Plate: Options include standard 6-, 12-, 24-, 48-, 96-, and 384-well plates, Cuvette (if

equipped), and Take3 (if configured).

Start WL and End WL: Define the start and end wavelengths. The full spectral range is

200 to 999 nm.

Step Size: Define an increment (in nm) for measurement. For example, if the Start WL

is 200 and the Step Size is 10, measurements are taken at 200, 210, 220, and so on
until the End WL is reached.

The number of spectral data points cannot exceed 100.

Microplates and Take3 only:

Calibrate first: Set to Yes (optimum performance) to perform calibration at all of the

specified wavelengths when a plate read is initiated. Set to No (optimum speed) to
perform calibration at only those wavelengths not yet calibrated since the reader was
turned on.

Shake Before Read: Select a shake mode of Linear (side-to-side), Orbital (circle), or

Double Orbital (figure 8). Set a Duration (up to 2 hours 45 minutes) to shake the plate

before spectral scanning begins. Adjust the Frequency, if needed. The cycles per
minute updates as the slider moves. The measurement in mm indicates the distance
the carrier travels during the shake.

When finished, tap Save. The protocol name is added to the Main Menu.

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Run a Protocol

Kinetic protocols: If a temperature set point is defined in the protocol, you can turn the
incubator on manually by selecting Instrument > Temperature. Otherwise, the
incubator will turn on when you start the protocol. Wait until the set point is reached,
or tap Override to read the plate.

1. From the Main Menu, tap a protocol in the Most Recent list or under one of the

available tabs. The Run Protocol screen displays the defined parameters:

2. To read a partial plate, tap Full Plate and select the well(s) to read. Tap Save.

3. Press the blue lighted button on the front of the reader to eject the plate carrier.

4. Place the microplate on the carrier and tap Start.

When the read is finished, the results are displayed and ready for output according to
the settings defined under Instrument > Output.

The reader saves results for the last 10 Endpoint/Take3 and last 2 Kinetic/Spectral
protocols that were run. The next set of saved results will overwrite the oldest set for
the read mode only with your acknowledgment.

Edit, Delete, or Copy a Protocol

Edit a Protocol

1. In the Main Menu, tap Protocol.

2. Tap the protocol that you want to modify, then tap Edit.

3. Make any desired changes, then tap Save.

Delete a Protocol

1. From the Main Menu, tap Protocol.

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

2. Tap the protocol you want to delete, then tap Delete.

Copy a Protocol

1. In the Main Menu, tap Protocol.

2. Tap the protocol you want to copy, then tap Copy.

3. Enter a name for the new protocol, then tap Save.

4. Make any desired changes, then tap Save.

View or Output Results Stored on the Reader

1. In the Main Menu, tap Results. Results for the last 10 Endpoint/Take3 and last 2

Kinetic/Spectral protocols are listed by read mode/file name. Tap the protocol for
which you want to view or output results. The results are displayed on the
touchscreen.

• Tap the data set name at the top of the screen to view protocol details,

including the start date/time, final status, and protocol parameters.

• Endpoint: Tap the screen to toggle through the available data sets.

• Kinetic/Spectral: Tap a well to view its kinetic curve or spectral scan.

• If a well displays “OVR,” the measurement exceeds (overflow) the maximum

absorbance measurement range: 4.000 OD (normal read speed, or cuvette);

3.000 OD (sweep read speed).

• If a well displays “???,” a result (for example, blank subtraction) could not be

calculated.

2. Tap the green button in the upper-right corner of the display. The results are printed

and/or saved to the USB flash drive, depending on the read mode and defined output
format.

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Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Run a Take3 Session

Using the Touchscreen

The Epoch 2 is preprogrammed with seven Take3 protocols, available for use after a

Take3 plate has been defined and aligned on the reader.

The protocols measure at 260, 280, and 320 nm and cannot be edited. For the nucleic
acid protocols, a secondary wavelength of 230 nm can be selected at run time. For all
seven protocols, a spectral scan may also be selected.

Protocol name

Mass Extinction

coefficient

Secondary Ratio WL

(run time option)

Spectral Scan

(run time option)

dsDNA

50

230 nm

240–300 nm

RNA

40

230 nm

240–300 nm

ssDNA

33

230 nm

240–300 nm

1 Abs@1cm =

1 mg/mL

10

-

260–320 nm

BSA

6.7

-

260–320 nm

IgG

13.7

-

260–320 nm

Lysozyme

26.4

-

260–320 nm

Define and Align a Take3 Plate

Ensure that the glass slide is clean before running the alignment procedure.
Refer to the Take3/Take3 Trio User Guide for cleaning instructions.

If you remove or replace the glass slide, perform the Pathlength Calibration
procedure described in the Take3/Take3 Trio User Guide and then run the
alignment procedure described below. Update the Pathlength Values on the
touchscreen with the new values generated through the Pathlength Correction
procedure described in the user guide.

The reader can be configured with only one Take3 plate at a time.

This procedure ensures that the Take3 slide's microspot positions are captured
correctly in the reader's software. Upon completion, the Take3 tab appears on the

Main Menu and contains the preprogrammed Take3 protocols.

1. From the Main Menu, tap Instrument and select the Take3 tab.

Getting Started Page 31 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

2. Enter the plate’s Serial Number.

3. Place the plate on the carrier. Tap Required (or Realign) and then Start to perform the

alignment. The process takes several minutes to complete.

4. Change the Blank threshold CV% if the default value does not meet your assay

requirements. This setting compares all replicates of a blank to determine cleanliness
of the microspots (dirty microspots can skew the %CV). Set the value smaller to have a
tighter tolerance on the deviation of cleanliness of the microspots, or set it higher to
relax the tolerance.

5. Tap Edit and enter the pathlength values that either came with the Take3 plate or

were generated through the Pathlength Correction procedure in the Take3 User
Guide.

6. When finished, tap Back and Home to return to the Main Menu.

Run a Take3 Protocol

1. On the Main Menu, tap the Take3 tab and select the protocol to run.

2. Select 1 to 12 wells as Blank and tap Continue.

3. On the Run Protocol screen, select options as applicable:

• Concentration units: Select the unit of measure for the export file.

• Secondary Ratio WL (230 nm): Available for nucleic acid protocols.

Getting Started Page 32 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

• Spectral Scan: Perform a spectral scan for the wavelength range displayed (1

nm step size), in addition to the endpoint reads.

.

4. Place the Take3 plate on the carrier and tap Start.

• If the Take3 Blank Masking screen appears and reports that the CV Status is

DIVERGING, the %CV of the blank wells is higher than the Blank threshold CV%

defined for the plate. Invalid blank wells are highlighted in red. Tap a well to
mask (exclude) it from blank average and %CV calculations. When the
remaining unmasked blank wells are valid, the Approve button illuminates. If
the number of valid blank wells is sufficient for the assay, tap Approve,
otherwise select Cancel and address the problem (e.g., clean the slides).

• At least one blank well is required. If all blank wells are masked, the CV Status is

INVALID.

• Each masked blank well will be flagged with an asterisk in the printed and

exported results.

5. When the read is finished, the results are displayed and ready for output according to

the settings under Instrument > Output.

Maintenance Page 33 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Maintenance

Schedule

Task

Frequency

Clean external surfaces

As needed

Inspect/clean touchscreen

As needed

Decontamination

Before shipment or storage

Clean Exposed Surfaces

Materials

• Deionized or distilled water

• Mild detergent

• Clean, lint-free cotton cloths

• Protective gloves

• Lab coat

• Biohazard trash bags

• Safety glasses

• Surgical mask

Procedure

1. Eject the plate carrier and then turn off and unplug the instrument.

2. Moisten a clean cotton cloth with water, or with water and mild detergent. Wring out

the cloth so that liquid does not drip from it. Do not soak the cloth.

3. Wipe the plate carrier, the inside of the plate carrier door, and all exposed surfaces of

the instrument.

4. If the reader is equipped with a cuvette holder, remove the holder and clean its

exposed surfaces.

5. If detergent was used, wipe all surfaces with a cloth moistened, not soaked, with

water.

6. Use a clean, dry, lint-free cloth to dry all wet surfaces.

If liquid is spilled inside the reader, contact BioTek Technical Assistance Center.

Maintenance Page 34 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Clean the Touchscreen

Do not use:

• Strong solvents, such as alcohol, acetone, ammonium chloride, methylene

chloride, and hydrocarbons, which can permanently damage the surface
of the touchscreen.

• Fibrous materials, such as paper towels, which can scratch the

touchscreen. Over time, dirt particles and cleaning agents can get trapped
in the scratches.

Materials

• Dish soap or other mild cleaner

• Deionized or distilled water

• Lint-free disposable towels

• Protective gloves

• Lab coat

• Biohazard trash bags

• Safety glasses

• Surgical mask

Procedure

Do not spray or soak the touchscreen! This will cause damage.

1. Eject the plate carrier and then turn off and unplug the instrument.

2. Moisten a clean lint-free disposable towel with water, or with water and dish soap.

Wring out the towel so that liquid does not drip from it. Do not soak the towel.

3. Wipe the touchscreen gently with the moist towel.

4. If detergent was used, wipe the touchscreen with a towel moistened, not soaked,

with water.

5. Dry the screen gently using another towel.

Maintenance Page 35 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Decontamination

Any laboratory instrument that has been used for research or clinical analysis is
considered a biohazard and requires decontamination prior to handling.

Decontamination minimizes the risk to all who come into contact with the instrument
during shipping, handling, and servicing. Decontamination is required by the U.S.
Department of Transportation regulations.

Persons performing the decontamination process must be familiar with the basic setup
of the instrument.

Turn off and unplug the instrument for the decontamination procedure.

BioTek Instruments, Inc., recommends the use of the following

decontamination solutions and methods based on our knowledge of the
instrument and recommendations of the Centers for Disease Control and
Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the
adequacy of these solutions and methods. Each laboratory must ensure that
decontamination procedures are adequate for the biohazard(s) they handle.

Wear prophylactic gloves when handling contaminated instruments. Gloved
hands should be considered contaminated at all times; keep gloved hands
away from eyes, mouth, and nose. Eating and drinking while decontaminating
instruments is not advised.

Mucous membranes are considered prime entry routes for infectious agents.
Wear eye protection and a surgical mask when there is a possibility of aerosol
contamination. Intact skin is generally considered an effective barrier against
infectious organisms; however, small abrasions and cuts may not always be
visible. Wear protective gloves when performing the decontamination
procedure.

Touchscreen models: Do not spray the sodium hypochlorite solution or
alcohol solution directly on the touchscreen. Avoid fibrous materials that can
scratch the touchscreen surface. Do not use a stronger cleaning solution than
recommended.

Materials

• Sodium hypochlorite (NaClO) or 70% isopropyl alcohol

• Deionized or distilled water

• 125-mL beakers

• Clean, lint-free cotton cloths

• Protective gloves

• Lab coat

Maintenance Page 36 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

• Biohazard trash bags

• Safety glasses

• Surgical mask

Procedure

1. Eject the plate carrier and then turn off and unplug the instrument.

2. Prepare an aqueous solution of 0.5% sodium hypochlorite (NaClO). If the effects of

sodium hypochlorite are a concern, 70% isopropyl alcohol may be used.

3. Moisten a clean, lint-free cloth with the cleaning solution. Wring out the cloth so that

liquid does not drip from it. Do not soak the cloth.

4. Wipe the plate carrier and all exposed surfaces of the instrument, including the inside

of the microplate carrier access door and, if equipped, the cuvette holder.

5. If the instrument is equipped with a touchscreen, place the moistened cloth on the

touchscreen and it let rest there for 15 minutes, then remove it.

6. Allow the instrument to dry for 20 minutes for thorough decontamination.

7. Moisten a cloth with deionized or distilled water and wipe all surfaces of the

instrument that have been cleaned with the solution. Do not soak the cloth.

8. Use a clean, dry lint-free cloth to dry all wet surfaces.

9. Discard the used gloves and cloths, using a biohazard trash bag and an approved

biohazard container.

Instrument Testing Page 37 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Instrument Testing

System Test

Each time the Epoch 2 is turned on, it automatically performs a series of tests on the

reader’s motors, lamp, and various subsystems. This test can take a few minutes to

complete. If all tests pass, the microplate carrier is ejected and the green LED on the
power switch remains on. The Main Menu appears on the touchscreen (if equipped).
You can also initiate a system test through the touchscreen or Gen5.

If any test fails, the reader beeps repeatedly and, if equipped, an error message
appears on the touchscreen. If this occurs, either press the carrier eject button, or, if
equipped, tap OK on the touchscreen to stop the beeping. If necessary, start another
system test to try to retrieve an error code from the reader.

Absorbance Test Plates

Gen5 software is required for this test.

Absorbance Test Plate Part Number 7260522 uses NIST-traceable neutral density
filters to confirm absorbance specifications in the visible range (400–800 nm). This test
plate also contains precision-machined holes to verify mechanical alignment.

Absorbance Test Plate Part Number 7260551 uses NIST-traceable neutral density
filters to test performance at 340 nm.

Define the Absorbance Test Plate Parameters

Before the Absorbance Plate Test can be performed, the wavelength settings and the
calibration data for each wavelength selected must be initially entered into Gen5. Use
the data sheet included with the Absorbance Test Plate for the following:

• Select System > Diagnostics > Test Plates > Add/Modify Plates, then click Add.

Click Help for guidance when setting the wavelengths and entering the OD and
peak wavelength values.

Run the Absorbance Plate Test

1. From the Gen5 main screen, click System > Diagnostics > Test Plates > Run. If

prompted, select the desired Test Plate and click OK.

2. When the Absorbance Test Plate Options dialog appears, select Perform Peak

Wavelength Test, if it is not already selected.

3. Highlight the wavelength(s) to be included in this test.

4. (Optional) Enter a comment.

Instrument Testing Page 38 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

5. Click Start Test.

6. Place the Absorbance Test Plate on the microplate carrier, with well A1 in the

proper location.

7. Click OK to run the test.

8. When the test is complete, the results report appears. Scroll through the report;

every result should show “PASS.”

Specifications Page 39 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Specifications

General Specifications

Microplates

The Epoch 2 accommodates standard 6-, 12-, 24-, 48-, 96-, and 384-well microplates with 128 x 86
mm geometry and the Take3 Micro-Volume Plate. If using Gen5, the Take3 Trio Micro-Volume plate
and BioCell are also supported.

Maximum plate height: 20.32 mm

Hardware and Environmental

Weight:

Fully configured weight ~11.3 kg

Dimensions:

Touchscreen models: 31.8 cm x 32.4 cm x 39.4 cm
Non-touchscreen models: 21.6 cm x 32.4 cm x 39.4 cm

Operational Temperature
Range:

Touchscreen models: 18°C to 30°C
Non-touchscreen models: 18°C to 40°C

Humidity:

10% to 85% relative humidity (non-condensing)

Power Consumption:

Powered from an external 120W (minimum), 24VDC power supply
compatible with 100-240 volts AC @50-60Hz.

Temperature Control

Range:

Maximum incubation temperature 65°C. Minimum setpoint must be
ambient room temperature plus 4°C. For incubation setpoints > 60°C,
ambient room temperature must be at least 22°C.

Uniformity:

+/- 0.5°C at 37°C, tested with Innovative Instruments, Inc. temperature
test plate.

Specifications Page 40 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Absorbance Specifications

For the performance specifications described in this section, the gain on the optics test
should be < 8.

Unless indicated otherwise, the specifications in the table below apply to both
microplate and cuvette.

Wavelength Selection System:

Monochromator

Detectors:

2 photodiodes (measurement & reference) each for the
plate channel and the cuvette channel

Wavelength Range:

200–999 nm

Bandpass:

< 5 nm (microplate & cuvette)

Measurement Range:

0.000–4.000 OD (microplate normal mode, cuvette)

0.000–3.000 OD (microplate sweep mode)

Resolution:

0.001 OD (touchscreen control only)

0.0001 OD (Gen5 control only)

Increment:

1 nm

Wavelength Accuracy:

± 2 nm

Wavelength Precision

0.2 nm (standard deviation)

Accuracy

Tested with certified neutral density glass

96-well plate
normal read speed
delay after plate movement = 100 ms

0.000 to 2.000 OD: ±1.0% ±0.010 OD

2.000 to 2.500 OD: ±3.0% ±0.010 OD

384-well plate
normal read speed
delay after plate movement = 100 ms

0.000 to 1.500 OD: ±2.0% ±0.010 OD

1.500 to 2.000 OD: ±5.0% ±0.010 OD

96- and 384-well plate
sweep read speed

0.000 to 1.000 OD: ±1.0% ±0.010 OD

Specifications Page 41 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Linearity

By liquid dilution, 200 µL volume in 96-well plate, 80 µL volume in 384-well plate

96-well plate
normal read speed
delay after plate movement = 100 ms

0.000 to 2.000 OD: ±1.0% ±0.010 OD

2.000 to 2.500 OD: ±3.0% ±0.010 OD

384-well plate
normal read speed
delay after plate movement = 100 ms

0.000 to 1.500 OD: ±2.0% ±0.010 OD

1.500 to 2.000 OD: ±5.0% ±0.010 OD

96- and 384-well plate
sweep read speed

0.000 to 1.000 OD: ±1.0% ±0.010 OD

Repeatability (Standard Deviation [STD])

Tested with certified neutral density glass, measured by one standard deviation
(8 measurements/data point)

96-well plate
normal read speed
delay after plate movement = 100 ms

0.000 to 2.000 OD: ±1.0% ±0.005 OD

2.000 to 2.500 OD: ±3.0% ±0.005 OD

384-well plate
normal read speed
delay after plate movement = 100 ms

0.000 to 1.500 OD: ±1.0% ±0.005 OD

1.500 to 2.000 OD: ±3.0% ±0.005 OD

96- and 384-well plate
sweep read speed

0.000 to 1.000 OD: ±2.0% ±0.010 OD

Assay Validation

Using a Take3 plate

260 nm dsDNA detection limit: < 5 ng/μL

Cuvette

Absorbance performance of measurements made via cuvette shall satisfy the
requirements given above for accuracy, linearity. and repeatability in a 96 well-plate,
normal read speed, 100 ms delay after movement.

Specifications Page 42 of 42

Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.

Reading Speeds

Endpoint

Start of plate motion to stop of plate motion

96 well

450 nm

[sec] MAX

Delay Time

[msec]

Normal Mode

74

100

Normal Mode, Min Delay Time

64

0

Sweep

35

0 (at end of row)

384 well

Normal Mode

192

100

Normal Mode, Min Delay Time

154

0

Sweep

42

0 (at end of row)

Kinetic

A1 to A1

96 well

450 nm

[sec] MAX

Delay Time

[msec]

Normal Mode

50

100

Normal Mode, Min Delay Time

40

0

Sweep

8

0 (at end of row)

384 well

Normal Mode

170

100

Normal Mode, Min Delay Time

131

0

Sweep

14

0 (at end of row)