Biotek ELx50 User manual
Operator’s Manual
All Models
Microplate Strip Washer
ELx50
™
.
ELx50™
Microplate Strip Washer
Operator’s Manual
January 2008
© 2008
Part Number 4071000
Revision L
BioTek® Instruments, Inc.
ii | Preface
Notices
BioTek Instruments, Inc.
®
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
All Rights Reserved
© 2008, BioTek® Instruments, Incorporated. No part of this publication may be
reproduced, transcribed, or transmitted in any form, or by any means electronic or
mechanical, including photocopying and recording, for any purpose other than the
purchaser’s use without written permission of BioTek Instruments, Inc.
Trademarks
BioTek® is a registered trademark, and ELx50™ and Liquid Level Alert™ are
trademarks of BioTek Instruments, Inc. Microsoft
trademarks or trademarks of Microsoft Corporation in the United States and/or
other countries. All other trademarks are the property of their respective holders.
Restrictions and Liabilities
Information in this document is subject to change and does not represent a
commitment by BioTek Instruments, Inc. Changes made to the information in this
document will be incorporated in new editions of the publication. No
responsibility is assumed by BioTek for the use or reliability of software or
equipment that is not supplied by BioTek or its affiliated dealers.
®
and Excel® are either registered
BioTek Instruments, Inc.
Contents
Preface................................................................................................i
Notices .......................................................................................... ii
All Rights Reserved..................................................................... ii
Trademarks ............................................................................... ii
Restrictions and Liabilities............................................................ ii
Contact Information........................................................................ ix
Customer Service and Sales........................................................ ix
Service/TAC.............................................................................. ix
European Coordination Center/Authorized European Representative.. ix
Document Conventions .................................................................... x
Revision History ............................................................................. xi
Intended Use Statement.................................................................xiv
Quality Control..............................................................................xiv
Repackaging and Shipping..............................................................xiv
Warnings......................................................................................xiv
Hazards and Precautions .................................................................xv
CE Mark Information..................................................................... xvii
Electromagnetic Interference and Susceptibility ............................... xviii
User Safety ................................................................................ xviii
Safety Symbols.............................................................................xix
Chapter 1: Introduction.....................................................................1
Introducing the ELx50 Microplate Strip Washer....................................2
ELx50 Washer Variations.................................................................. 3
Manifold Variations......................................................................3
Patented Dual-Manifold Design................................................. 4
Carrier Variations........................................................................5
Software Variations.....................................................................5
Hardware Features .......................................................................... 7
Accessories Included........................................................................8
Optional Accessories ........................................................................9
Specifications................................................................................ 10
Microplates .............................................................................. 10
Electrical ................................................................................. 10
Physical................................................................................... 10
Environmental.......................................................................... 10
Hardware ................................................................................ 10
Performance Specifications......................................................... 11
Product Support & Service.............................................................. 12
Contacting the Technical Assistance Center .................................. 12
Returning Instruments for Service/Repair..................................... 12
Contents | iii
ELx50 Operator's Manual
iv | Preface
Chapter 2: Installation .................................................................... 13
Unpacking and Inspecting the Instrument......................................... 14
Unpacking the 16-Channel Manifold, Mist Shield, and Bottles .......... 15
Unpacking the 8-, 8s-, or 12-Channel Manifold, Microplate Carrier,
Power Supply, Tubing, In-Line Filter, and Optional Pump Muffler...... 16
Unpacking the Inner Shipping Container and Foam Corner Blocks.... 17
Unpacking the Aspiration Carrier (ELx50/8FMW models only).......... 18
Unpacking the ELx50 Washer ..................................................... 19
Setting Up the ELx50 ..................................................................... 20
Operating Environment.............................................................. 20
Installing the Carrier and Manifold............................................... 20
Microplate Carrier................................................................. 20
8-, 8s-, or 12-Channel Manifold.............................................. 21
16-Channel Manifold............................................................. 22
Aspiration Carrier (ELx50/8FMW) ........................................... 23
Connecting the Tubes and Bottles ............................................... 25
Before Connecting the Tubes and Bottles................................. 25
Waste System ..................................................................... 28
Supply System .................................................................... 31
Liquid Level Alert Option ....................................................... 34
Final Check ......................................................................... 35
Attaching the Mist Shield ........................................................... 35
Connecting the Power Supply and Cord........................................ 36
Serial Port for Communication With Other Devices......................... 36
Verifying Performance.................................................................... 37
Repackaging and Shipping.............................................................. 38
Before Repackaging the Instrument............................................. 38
Repackaging the ELx50 and Its Accessories.................................. 38
Removing the Mist Shield...................................................... 39
Removing the Manifold and Microplate Carrier.......................... 40
Removing the Aspiration Carrier (ELx50/8FMW) ....................... 41
Packing the 8-, 8s-, or 12-Channel Manifold in Its Container ...... 42
Packing the 16-Channel Manifold in Its Container...................... 43
Rotating the Cam................................................................. 44
Placing the Washer into the Inner Shipping Container................ 45
Placing the Aspiration Carrier into the Inner Shipping Container.. 46
Packing the Inner Shipping Container...................................... 47
Packing the 16-Channel Manifold and Mist Shield...................... 48
Packing the 8-, 8s-, or 12-Channel Manifold, Microplate Carrier,
and Power Supply ................................................................ 49
Chapter 3: Operation.......................................................................51
ELx50 Front Panel.......................................................................... 52
Keypad Description........................................................................ 53
Entering Program Names................................................................ 54
BioTek Instruments, Inc.
Contents | v
System Startup............................................................................. 54
Main Menu.................................................................................... 55
General Usage Guidelines ............................................................... 56
Before Running Any Program...................................................... 56
Maintenance ............................................................................ 58
Programming Considerations...................................................... 58
Liquid Level Alert Option ....................................................... 59
Wash........................................................................................... 60
Run Wash Program ................................................................... 60
Run Wash Program Options................................................... 60
Define Wash Program................................................................ 61
Define Wash Program Options................................................ 61
Define Wash METHOD Program Options................................... 62
Wash METHOD Defaults and Ranges ....................................... 63
Define Wash DISPENSE (DISP) Program Options ...................... 64
Wash DISPENSE Defaults and Ranges..................................... 66
Define Wash ASPIRATION (ASPIR) Program Options ................. 67
Wash ASPIRATE Defaults and Ranges...................................... 69
Edit Wash Program ................................................................... 71
Copy Wash Program .................................................................. 71
Copy Wash Program Options................................................. 71
Delete Wash Program................................................................ 71
Delete Wash Program Options............................................... 71
Prime........................................................................................... 72
Define Prime Program ............................................................... 72
Define Prime Program Options ............................................... 72
PRIME Program Defaults and Ranges ...................................... 73
Run Prime Program................................................................... 74
Run Prime Program Options.................................................. 74
Edit Prime Program................................................................... 74
Copy Prime Program ................................................................. 75
Copy Prime Program Options ................................................ 75
Delete Prime Program ............................................................... 75
Delete Prime Program Options .............................................. 75
Dispense...................................................................................... 76
Define Dispense Program........................................................... 76
Define Dispense Program Options.......................................... 76
Run Dispense Program .............................................................. 76
Run Dispense Program Options ............................................. 77
Edit Dispense Program .............................................................. 77
Copy Dispense Program............................................................. 77
Copy Dispense Program Options............................................ 78
Delete Dispense Program........................................................... 78
ELx50 Operator's Manual
vi | Preface
Delete Dispense Program Options.......................................... 78
Aspiration..................................................................................... 79
Define Aspiration Program.......................................................... 79
Define Aspiration Program Options ......................................... 79
Define Aspirate-only Program Options..................................... 80
Aspirate-only Program Defaults and Ranges............................. 80
Run Aspiration Program............................................................. 80
Run Aspiration Program Options............................................. 81
Edit Aspiration Program............................................................. 81
Copy Aspiration Program ........................................................... 81
Copy Aspiration Program Options ........................................... 82
Delete Aspiration Program.......................................................... 82
Delete Aspiration Program Options ......................................... 82
Soak............................................................................................ 83
Define Soak Program................................................................. 83
Define Soak Program Options ................................................ 83
Edit Soak Program.................................................................... 84
Copy Soak Program .................................................................. 84
Copy Soak Program Options .................................................. 84
Delete Soak Program................................................................. 85
Delete Soak Program Options ................................................ 85
Linking Programs........................................................................... 86
Define Link Program.................................................................. 86
Define Link Program Options.................................................. 86
View Link Program.................................................................... 87
Run Link Program ..................................................................... 87
Run Link Program Options.................................................... 88
Delete Link Program.................................................................. 88
Delete Link Program Options................................................. 88
Utility .......................................................................................... 89
Tests ...................................................................................... 89
Self-Test (SLFCHK) .............................................................. 89
Configuration Information (CHKSUM)...................................... 89
Setup...................................................................................... 90
Manifold (MANIFLD) ............................................................. 90
SENSOR ............................................................................. 90
AutoPrime................................................................................ 91
ELx50™ Microplate Strip Washer Menu Maps ..................................... 92
Chapter 4: Maintenance and Performance Verification
(IQ/OQ/PQ).....................................................................................97
Overview...................................................................................... 98
Recommended Maintenance and Performance Verification Schedules .... 98
Recommended Maintenance Schedule .............................................. 99
Recommended Performance Verification Schedule.............................100
BioTek Instruments, Inc.
Contents | vii
Warnings & Precautions.................................................................101
Maintenance Programs..................................................................102
Prime Programs ...........................................................................103
Daily Maintenance ........................................................................104
DAY_RINSE.............................................................................104
AUTOPRIME ............................................................................104
AutoPrime Program Options..................................................105
Overnight Maintenance..................................................................106
OVERNIGHT_LOOP...................................................................106
RINSE_AND_SOAK...................................................................107
Monthly Maintenance ....................................................................108
Tools and Supplies...................................................................108
Cleaning the Bottles.................................................................109
Cleaning the Manifold...............................................................109
Cleaning the Aspirate and Dispense Tubes...................................110
8-, 8s-, or 12-Channel Manifold.............................................110
16-Channel Manifold............................................................110
Cleaning the Carrier.................................................................111
Microplate Carrier................................................................111
Aspiration Carrier (ELx50/8FMW only)....................................112
Cleaning the Check Valves ........................................................114
Long-Term Storage Maintenance ....................................................115
LONG_SHUTDOWN...................................................................115
Disinfect the Aspiration Carrier (ELx50/8FMW).............................116
Storing the ELx50™......................................................................116
Decontamination ..........................................................................117
Purpose..................................................................................117
Tools and Supplies...................................................................118
Decontamination Procedure for External Components....................119
Decontamination Procedure for Tubing and Manifold .....................119
Alternate Decontamination Procedure for Tubing and Manifold........120
Decontamination Procedure for Aspiration Carrier (ELx50/8FMW) ... 120
Performance Verification................................................................122
Installation Qualification (IQ) .........................................................123
Operational Qualification (OQ)........................................................123
Performance Qualification (PQ).......................................................124
Self-Test and Checksum Test .........................................................125
Self-Test ................................................................................125
Configuration Information (CHKSUM)..........................................125
Liquid Tests for the 8-, 8s- and 12-Channel Manifolds........................126
Evacuation Efficiency and Dispense Precision Tests.......................126
Materials............................................................................126
Evacuation Efficiency Test Procedure......................................127
Dispense Precision Test Procedure.........................................128
ELx50 Operator's Manual
viii | Preface
Liquid Tests Worksheet (8-, 8s-, and 12-Channel Manifolds) ..........129
Liquid Tests for the 16-Channel Manifold..........................................130
Evacuation Efficiency and Dispense Precision Tests.......................130
Materials............................................................................130
Evacuation Efficiency Test Procedure......................................131
Dispense Precision Test Procedure.........................................132
Liquid Tests Worksheet (16-Channel Manifold).............................133
Liquid Test for the Aspiration Carrier (ELx50/8FMW)..........................134
Evacuation Efficiency Test.........................................................134
Materials............................................................................134
Evacuation Efficiency Test Procedure......................................134
Liquid Test Worksheet (Aspiration Carrier)...................................136
Maintenance/Performance and Operational Logs ...............................137
Chapter 5: Troubleshooting...........................................................141
Overview.....................................................................................142
Troubleshooting Checklist .........................................................142
Troubleshooting Charts .................................................................143
Washer Start-Up......................................................................143
Fluid Aspiration .......................................................................144
Fluid Delivery..........................................................................148
Fluid Leakage.......................................................................... 150
Carrier Movement....................................................................151
Microplate Carrier................................................................151
Aspiration Carrier (ELx50/8FMW) ..........................................151
Washer Manifold Movement....................................................... 151
Microplate Scratches ................................................................152
Syringe Drive Movement...........................................................152
Appendix A: Error Codes................................................................153
Error Codes .................................................................................154
General Errors .............................................................................155
Fatal Errors .................................................................................157
Dispense Volume Invalid for Manifold Type (0F00 Error).....................158
Appendix B: ELx50 Manifold Variations ......................................... 159
ELx50 Manifold Variations..............................................................160
Reconfiguring the Washer .........................................................160
Hardware Steps.......................................................................161
Software Steps........................................................................162
Appendix C: Chemical Compatibility ..............................................163
Components and Materials Composition...........................................164
Appendix D: Default Programs ......................................................165
Default Programs..................................................................... 166
BioTek Instruments, Inc.
Contact Information
BioTek® Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
Customer Service and Sales
Internet: www.biotek.com
Phone: 888-451-5171 (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: 802-655-7941
E-Mail: customercare@biotek.com
Contact Information | ix
Service/TAC
Phone: 800-242-4685 (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: 802-655-3399
E-Mail: tac@biotek.com
European Coordination Center/Authorized European Representative
BioTek® Instruments GmbH
Kocherwaldstrasse 34
D-74177 Bad Friedrichshall
Germany
Internet: www.biotek.de
Phone: +49 (0) 7136 9680
Fax: +49 (0) 7136 968 111
E-Mail: info@biotek.de
ELx50 Operator's Manual
x | Preface
Document Conventions
This manual uses the following typographic conventions:
Example Description
This icon calls attention to important safety notes.
Warning!
A Warning indicates the potential for bodily harm and tells you
how to avoid the problem
.
Caution
DEFINE
Note:
A Caution indicates potential damage to the instrument and tells
you how to avoid the problem.
Text in COURIER font represents menu options as they appear on
the instrument’s display.
Bold text is primarily used for emphasis.
This icon calls attention to important information.
About This Manual
The intent of this Operator’s Manual is to instruct the new user how to set up and
operate BioTek’s ELx50™. To help you read and understand this manual, certain
document conventions have been used.
Major topic headings start a new page (such as
give you a visual and style clue that a new major subject is being introduced. One or
more subheadings may appear below each major heading.
Document Conventions, above) to
BioTek Instruments, Inc.
Revision History
Revision Date Changes
A 6/97 First Issue.
B 9/97
C 4/99 Updated menu sequences, addresses.
D 4/99 Revised operator’s manual to reflect BOM revision.
E 6/99 Added support for 16-channel manifold/384-well plates.
F 3/01
G 10/03
H 2/04
I 2/07
Added valve tubing connection diagram. Corrected rear panel
figure.
Revised Chapter 4 maintenance procedures; removed
Appendix B, Performance Verification, and added it to
Chapter 4. Added a log to record maintenance.
Updated Notices to include current company contact
information. Updated Safety Symbols and Intended Use.
Added Chemical Compatibility information to Precautions.
Updated Warranty to include Bio-Tek’s current warranty
statement. Changed operating environment temperature
range from 15°C - 30°C to 15°C - 40°C. Added Registration
Card information. Clarified bleach dilutions for
Decontamination.
Reformatted entire manual according to new template.
Changed selectable ranges for the following throughout
manual to conform to software specs: Prime Volume from 1
to 999 ml to 1 to 200 ml; Shake Duration from 0 to 600
seconds to 1 to 600 seconds, Bottom Dispense Volume from
25 to 3000 µl/well to 50 to 3000 µl/well. Updated
introductory material, enhanced Hardware Features, Package
Contents, and Optional Accessories. Corrected specs for
physical dimensions and storage and operating temperatures.
Updated contact information, enhanced Unpacking and
Packing instructions with new text and 14 new figures.
Enhanced Operation chapter with new sections: Keypad
Description, System Startup, Main Menu, and Utility. Added
new Defaults and Ranges tables for Wash Method, Wash
Dispense, Wash Aspirate, and Prime Programs. Restructured
Chapter 4, Maintenance and Performance Verification for
continuity, enhanced Decontamination section, updated
Liquid Testing section. Enhanced/updated Troubleshooting
and Error Codes. Added two new appendices C, Chemical
Compatibility, and D, Default Programs.
Added instructions and information in all chapters of the
manual for the aspiration carrier (ELx50™/8FMW model).
Removed hyphen from “Bio-Tek” (“BioTek”) and changed
“ELx50 Automated Strip Washer” to “ELx50 Microplate Strip
Washer.” Replaced the previous cover with new cover design
from Marketing.
Cont’d on next page
Revision History | xi
ELx50 Operator's Manual
xii | Preface
Revision Date Changes
Updated regulatory/safety information and removed
Warranty and Registration Card sections in the Preface.
Revised ranges for prime flow rate, dispense flow rate,
bottom flow rate, and dispense volume for the 12-channel
manifold in Chapter 3, to reflect recent revisions in the
washer’s software. Revised instructions for flushing BSA in
Chapter 4. Incorporated manual update H2: added dispense
volume ranges for 96- and 384-well plates for the 16-channel
manifold in Chapter 3 and in Appendix A, and revised the
Recommended Maintenance/Performance Verification
Schedule in Chapter 4 by moving the Liquid Tests from the IQ
to the Initial/Annual OQ and monthly PQ.
J 4/07
K 10/07
Revised Chapters 1 through 5 primarily to support a redesign
of the aspiration carrier in the ELx50/8FMW washer: the
vacuum grate is now secured in place (under the plate seal
gasket) by six screws around the perimeter of the top of the
carrier. Also revised vacuum levels for the aspiration carrier:
from –240 mmHg to –64 mmH for the 0.047” diameter hole
of the carrier’s vent port, and from –64 mmHg to –240
mmHg for the 0.020” diameter hole in the black vent plug.
General. Added information on the Liquid Level Alert™ option,
which is available for all ELx50 models. Added information on
the new ‘First Strip’ run-time parameter. Additional cleanup
and clarifications as described below.
Chapter 1, Introduction. Software Variations: Clarified which
features and functions are supported for the different
basecode part numbers. Hardware Features: Simplified the
description of the supply and waste bottles provided with the
instrument. Optional Accessories: Added information on
Liquid Level Alert option.
Chapter 2, Installation. Connecting Tubes and Bottles: Added
information on the Liquid Level Alert option components.
Added Liquid Alert port to drawings. Added instructions for
connecting the sensor cables to the select box, and the select
box to the washer.
Chapter 3, Operation. Throughout: Added information on the
‘First Strip’ run-time parameter. Updated Menu Maps.
General Usage Guidelines: Added section for the Liquid Level
Alert option. Linking Programs: Added troubleshooting tip for
resolving Link program creation issues. Setup: Added
information on new SENSOR option.
Chapter 4, Maintenance/Performance Verification.
Recommended Maintenance and Performance Verification
Schedules: Divided the former Maintenance AND Performance
Verification Schedule table into two separate tables.
Decontamination: Added warning not to prepare a strongerthan-recommended bleach solution.
Appendix A, Error Codes. General Errors: Added error codes
2800 and 2900, related to the Liquid Level Alert option.
BioTek Instruments, Inc.
Revision Date Changes
L 1/08
General. Added information on the optional vacuum pump
muffler for ELx50 washers equipped with the new vacuum
pump (washers manufactured after January 2008). Additional
changes are described below.
Preface. Updated Directives in the CE Mark section and added
Unspecified Use Warning to Hazards and Precautions.
Chapter 1, Introduction. Added the optional vacuum pump
muffler to the Hardware Features and Accessories Included
sections. Updated the Performance Specifications section for
the 96-well: changed residual volume specs from ≤ 4.0 µl to
≤ 2.0 µl per well, and dispense precision specs from
≤ 4.0% CV to ≤ 3.0% CV.
Chapter 2, Installation. Updated unpacking/packing artwork
and instructions to reflect new packaging materials, and
added a graphic of the vacuum pump muffler to illustrations
of the washer’s rear panel.
Chapter 4, Maintenance and Performance Verification
(IQ/OQ/PQ). In the Evacuation Efficiency and Dispense
Precision Tests and Worksheets for the 8-, 8s-, and
12-channel manifolds, changed the residual volume specs
from ≤ 0.004 grams per well to ≤ 0.002 grams per well, and
the dispense precision specs from ≤ 4.0% CV to ≤ 3.0% CV.
Chapter 5, Troubleshooting. In the Troubleshooting Charts,
added references to the vacuum pump muffler wherever
references to the in-line vacuum filter were present.
Appendix B, Manifold Variations. Removed instructions for
returning washers to BioTek for reconfiguration to work with
another manifold. (The user can perform all the steps
necessary to reconfigure the washer for a different manifold.)
Revision History | xiii
ELx50 Operator's Manual
xiv | Preface
Intended Use Statement
• The ELx50™ Microplate Strip Washer provides microplate priming and washing
for ELISA™, fluorescence and chemiluminescence immunoassays, cellular and
agglutination assays.
• The intended use of this instrument is dependent on the instrument’s rear panel
label. If there is an IVD label, then the instrument may be used for clinical,
research and development, or other non-clinical purposes. If there is no such label,
then the instrument may
non-clinical purposes.
only be used for research and development, or for other
Quality Control
It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for the test
to be conducted. Failure to conduct Quality Control checks could result in erroneous test
data.
Repackaging and Shipping
If you need to ship the instrument to BioTek for service or
repair, contact BioTek for a Return Materials Authorization
(RMA) number, and be sure to use the original packing. Other
forms of commercially available packing are not recommended
and can void the warranty. If the original packing materials
have been damaged or lost, contact BioTek for replacement
packing.
Warnings
Operate the instrument on a flat surface and away from excessive humidity.
When operated in a safe environment according to the instructions in this
document, there are no known hazards associated with the ELx50™.
However, the operator should be aware of certain situations that could result
in serious injury; these may vary depending on the instrument model.
BioTek Instruments, Inc.
Hazards and Precautions
Hazards
Hazards and Precautions | xv
Warning! Power Rating.
supply must be connected to a power receptacle that provides voltage and
current within the specified rating for the system. Use of an incompatible
power receptacle may produce electrical shock and fire hazards.
Warning! Electrical Grounding.
connect primary power to the ELx50 power supply. Use of a two-prong
adapter disconnects the utility ground, creating a severe shock hazard.
Always connect the power cord directly to a three-prong receptacle with a
functional ground.
Warning! Internal Voltage.
unplug the power supply before cleaning the outer surface of the instrument.
Warning! Liquids.
into internal components creates a potential shock hazard. Wipe up all spills
immediately. Do not operate the instrument if internal components have
been exposed to fluid.
Warning! Potential Biohazards.
biohazard. Adequate safety precautions should be taken as outlined in the
assay’s package insert. Always wear safety glasses and appropriate
protective equipment, such as chemically resistant rubber gloves and apron.
Avoid spilling liquids on the instrument; fluid seepage
The ELx50 Microplate Strip Washer’s power
Never use a two-prong plug adapter to
Always turn off the power switch and
Some assays or specimens may pose a
Warning! Unspecified Use.
the guidelines and safeguards specified in this manual could result in a
Failure to operate this equipment according to
hazardous condition.
Warning! Software Quality Control.
manufacturer’s assay package insert when modifying software parameters
and establishing wash methods, using the washer’s onboard software.
Warning! Pinch Hazard.
operation, as the syringe pump may pinch your fingers.
Do not reach under the instrument during
The operator must follow the
ELx50 Operator's Manual
xvi | Preface
Precautions
The following precautions are provided to help avoid damage to the instrument:
Caution: Service.
The washer should be serviced by BioTek authorized
service personnel. Only qualified technical personnel should perform
troubleshooting and service procedures on internal components.
Caution: Environmental Conditions.
Do not expose the instrument to
temperature extremes. For proper operation, ambient temperatures should
remain between 15°-40°C (59°-104°F). Performance may be adversely
affected if temperatures fluctuate above or below this range. Storage
temperature limits are broader (see
Caution: Sodium Hypochlorite.
Storing the ELx50 in Chapter 4).
Do not expose any part of the
instrument to the recommended diluted sodium hypochlorite solution
(bleach) for more than 20 minutes. Prolonged contact may damage the
instrument surfaces. Be certain to rinse and thoroughly wipe all surfaces.
Caution: Chemical Compatibility - Washers.
Some chemicals may
cause irreparable damage to washers. The following chemicals have been
deemed safe for use in washers: buffer solutions (such as PBS), saline,
surfactants, deionized water, 70% ethyl, isopropyl, or methyl alcohol, 40%
formaldehyde, and 20% sodium hydroxide. Never use acetic acid, DMSO, or
organic solvents. Other chemicals may cause severe damage to the
instrument. Contact BioTek prior to using any other chemicals. Refer to
Appendix C, Chemical Compatibility.
Caution: Washers and Wash Buffer Solution.
Although many
precautions have been taken to ensure that the instrument is as corrosionproof as possible, the washer is not sealed and liquids can seep into sensitive
components. Make sure that any spilled wash buffer solution is wiped off the
washer. Prolonged exposure to salt solution may corrode parts of the carrier,
movement rail, springs, and other hardware.
Caution: Bovine Serum Albumin.
Solutions containing proteins, such as
bovine serum albumin (BSA), will compromise the washer’s performance
over time unless a strict maintenance protocol is adhered to. See
Maintenance and Performance Verification (IQ/OQ/PQ) for cleaning
instructions regarding BSA.
Caution: Warranty.
may
void the warranty. See Chapter 4.
Caution: Disposal.
This instrument contains printed circuit boards and
Failure to follow preventive maintenance protocols
Chapter 4,
wiring with lead solder. Dispose of the instrument according to Directive
2002/96/EC, “on waste electrical and electronic equipment (WEEE).”
BioTek Instruments, Inc.
Hazards and Precautions | xvii
Based on the testing described below and information contained
herein, this instrument bears the CE mark.
Directive 2004/108/EC: Electromagnetic Compatibility
Emissions - CLASS A
EN 50081-1 and IEC 61326-1
EN 55022 Class A
Immunity
EN 50082-1 and IEC 61326-1
IEC 1000-4-2 Electrostatic Discharge
IEC 1000-4-3 Radiated EM Fields
IEC 1000-4-4 Electrical Fast Transient/Burst
IEC 1000-4-5 Surge Immunity
EN 61000-4-6 Conducted Disturbances
EN 61000-4-11 Voltage Dips, Short Interruptions and Variations
Directive 73/23/EEC Low Voltage
EN 61010-1: “Safety requirement for electrical equipment for measurement, control
and laboratory use. Part 1, General requirements.”
Directive 2002/96/EC: Waste Electrical and Electronic Equipment
Disposal Notice
This instrument contains printed circuit boards and wiring with lead solder. Dispose of
the instrument according to Directive 2002/96/EC, “on waste electrical and electronic
equipment (WEEE).”
Directive 98/79/EC: In Vitro Diagnostics (some models)
• Product registration with competent authorities
• Traceability to the U.S. National Institute of Standards and Technology (NIST):
Dispense precision and average residual volume is traceable to NIST.
Specific data for a particular serial number is available upon request from BioTek
Instruments. See page ix for contact information.
ELx50 Operator's Manual
xviii | Preface
Electromagnetic Interference and Susceptibility
USA FCC CLASS A
Warning: Changes or modifications to this unit not expressly approved by the
manufacturer could
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to Part 15 of the FCC Rules.
These limits are designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial environment. Like all similar equipment,
this equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful
interference to radio communications. Operation of this equipment in a residential area is
likely to cause interference, in which case the user will be required to correct the
interference at his own expense.
Canadian Department of Communications Class A
void the user's authority to operate the equipment.
This digital apparatus does not exceed Class A limits for radio emissions from digital
apparatus set out in the Radio Interference Regulations of the Canadian Department of
Communications.
Le present appareil numerique n'met pas du bruits radioelectriques depassant les limites
applicables aux appareils numerique de la Class A prescrites dans le Reglement sur le
brouillage radioelectrique edicte par le ministere des Communications du Canada.
User Safety
This device has been type tested by an independent laboratory and found to meet the
requirements of the following:
North America
• Canadian Standards Association CAN/CSA C22 .2 No . 61010-1-04
“Safety Requirements for Electrical Equipment for Measurement, Control and
Laboratory Use, Part 1: General Requirements.”
• Underwriters Laboratories UL 61010-1:2004
“Safety Requirements for Electrical Equipment for Measurement, Control and
Laboratory Use, Part 1: General Requirements.”
International
• EN 61010-1
“Safety Requirements for Electrical Equipment for Measurement, Control and
Laboratory Use, Part 1: General Requirements.”
BioTek Instruments, Inc.
Safety Symbols
Safety Symbols | xix
Alternating current
Courant alternatif
Wechselstrom
Corriente alterna
Corrente alternata
Direct current
Courant continu
Gleichstrom
Corriente continua
Corrente continua
Both direct and alternating current
Courant continu et courant alternatif
Gleich - und Wechselstrom
Corriente continua y corriente alterna
Corrente continua e corrente alternata
Earth ground terminal
Borne de terre
Erde (Betriebserde)
Borne de tierra
Terra (di funzionamento)
Protective conductor terminal
Borne de terre de protection
Schutzleiteranschluss
Borne de tierra de protección
Terra di protezione
On (Supply)
Marche (alimentation)
Ein (Verbindung mit dem Netz)
Conectado
Chiuso
Off (Supply)
Arrêt (alimentation)
Aus (Trennung vom Netz)
Desconectado
Aperto (sconnessione dalla rete di alimentazione)
ELx50 Operator's Manual
xx | Preface
Caution (refer to accompanying documents)
Attention (voir documents d’accompanement)
Achtung siehe Begleitpapiere
Atención (vease los documentos incluidos)
Attenzione, consultare la doc annessa
Warning, risk of electric shock
Attention, risque de choc électrique
Gefährliche elektrische Schlag
Precaución, riesgo de sacudida eléctrica
Attenzione, rischio di scossa elettrica
Warning, risk of crushing or pinching
Attention, risque d’écrasement et pincement
Warnen, Gefahr des Zerquetschens und Klemmen
Precaución, riesgo del machacamiento y sejeción
Attenzione, rischio di schiacciare ed intrappolarsi
Warning, hot surface
Attention, surface chaude
Warnen, heiße Oberfläche
Precaución, superficie caliente
Attenzione, superficie calda
Consult instructions for use
Consulter la notice d’emploi
Gebrauchsanweisung beachten
Consultar las instrucciones de uso
Consultare le istruzioni per uso
In vitro diagnostic medical device
Dispositif médical de diagnostic in vitro
Medizinisches In-Vitro-Diagnostikum
Dispositivo médico de diagnóstico in vitro
Dispositivo medico diagnostico in vitro
Separate collection for electrical and electronic
equipment
Les équipements électriques et électroniques font l’objet
d’une collecte sélective
Getrennte Sammlung von Elektro- und Elektronikgeräten
Recogida selectiva de aparatos eléctricos y electrónicos
Raccolta separata delle apparecchiature elettriche ed
elettroniche
BioTek Instruments, Inc.
Chapter 1
Introduction
This chapter introduces the ELx50™ Microplate Strip Washer, and
describes its hardware and software features and technical
specifications. Instructions on how to contact BioTek for Product
Support & Service are included on page 12.
Introducing the ELx50 Microplate Strip Washer ............................. 2
ELx50 Washer Variations ........................................................... 3
Manifold Variations............................................................... 3
Patented Dual-Manifold Design............................................ 4
Carrier Variations ................................................................. 5
Software Variations .............................................................. 5
Hardware Features.................................................................... 7
Accessories Included ................................................................. 8
Optional Accessories ................................................................. 9
Specifications..........................................................................10
Microplates.........................................................................10
Electrical............................................................................10
Physical .............................................................................10
Environmental ....................................................................10
Hardware...........................................................................10
Performance Specifications ...................................................11
Product Support & Service ........................................................12
Contacting the Technical Assistance Center.............................12
Returning Instruments for Service/Repair ...............................12
2 | Chapter 1: Introduction
Introducing the ELx50 Microplate Strip Washer
Fast, versatile, and easy to use, the ELx50™ is a user-programmable, fully automated
microplate strip washer. Its key features include the following:
• The precision syringe pump with its long-lasting seal ensures precise fluid
delivery, as well as reproducibility from wash to wash.
• Programmable dispense volumes, flow, and height, and aspiration rates and
depths provide for a wide range of washing capabilities, from gentle washing for
cellular assays to vigorous washing for ELISA™.
• The optional bottom washing routine can be applied to lower the background
absorbance. The optional crosswise aspiration routine can be applied to reduce
residual volumes.
• The ELx50 can be used to wash all microplate-based assays, including ELISA,
fluorescence, chemiluminescence, RIA, DNA probes, and cellular assays.
• The intuitive menu-driven software allows you to create and store up to 75 washer
programs. When you are ready to run a program, simply select the program name
and follow the screen prompts. The ELx50 will then process the plate according to
the program parameters.
• All ELx50 models support Wash, Prime, Dispense, and Aspirate programs. Some
models also support Soak and Link programs.
• Several pre-defined programs are provided to simplify preventative maintenance,
which should be performed regularly to ensure optimum washer performance.
• All ELx50 models can be used to wash standard 96-well microplates, and some
models are also equipped to wash standard 384-well microplates.
• “V” versions of the ELx50 feature automatic wash buffer switching with three
valves.
• The Liquid Level Alert™ option allows users the convenience of continuous
monitoring for both supply and waste bottles. At the beginning and end of a wash
protocol, the liquid level is verified to ensure sufficient wash buffer remains in the
designated dispense bottle to complete a microplate wash. Sufficient storage
capacity in the waste bottle is also verified. Each Liquid Level Alert kit includes a
quantity of supply and waste bottles with level-sensing capability. Up to three
different supply bottles can be connected to the washer through the Liquid Level
Alert Select Box.
• An aspiration carrier is provided with the ELx50/8FMW model for bottom
aspiration of filter-bottom microplates. A vent port on the carrier and two
selectable vent plugs for altering the diameter of the port provide three vacuum
levels: low, medium, and high. Plates with filter pore sizes from 0.45 µm to 1.2 µm
are supported.
BioTek Instruments, Inc.
ELx50 Washer Variations | 3
ELx50 Washer Variations
There are several different ELx50™ washer models available: the ELx50/8, ELx50/12,
ELx50/16, and ELx50/8FMW.
Each washer comes with one of four possible washer manifolds (8-, 8s-, 12-, or 16-channel
positioned above the microplates), and “V” version washers include automatic buffer
switching with three valves. All models support the Liquid Level Alert™ option.
The washer manifold, carrier, and software variations are described on the next few pages.
For additional information, contact BioTek Instruments (see
page 12).
Manifold Variations
The manifold is the washer component positioned above the microplate that delivers
fluid to, and removes it from, the microwells. Each ELx50 washer is equipped with a
manifold containing 8, 12, or 16 sets of aspirate and dispense tubes, or “channels.” The
following table shows the washing capabilities of each manifold:
Product Support & Service,
With this manifold: You can wash:
8-channel 8-well strips, 96-well plates
8s-channel 8-well strips, 96-well plates
12-channel 12-well strips, 96-well plates
16-channel 8-well strips, 96-well plates, 384-well plates
The ELx50 supports the washing of standard flat, round, and ‘V’ bottom microwells;
the ELx50/8FMW model also supports bottom aspiration of filter-bottom plates.
The ELx50 can be reconfigured to support a different manifold. See
ELx50 Manifold Variations for more information.
The 8s-channel manifold has shorter dispense tubes for special
applications.
The 16-channel manifold uses two aspirate tubes for each well when
used with 8-well strips and 96-well plates. For these formats, it may be
difficult for the tubes to reach the very bottom of round or ‘V’ bottom
wells, possibly leading to higher residuals.
The ELx50/8FMW model does not support the 16-channel manifold.
Appendix B,
ELx50 Operator's Manual
4 | Chapter 1: Introduction
Patented Dual-Manifold Design
The patented Dual-Action™ 16-channel manifold has 16 sets of dispense and
aspirate tubes arranged in a fixed horizontal position relative to each other but
located in two separate manifolds (see
independently of each other on the vertical axis. This dual-manifold design
overcomes the problem of fitting both a dispense and an aspirate tube into the
much smaller 384-well at the same time, yet it allows for overfill washing of the
smaller 384 wells. The dispense tubes are held above the plate when the dispense
manifold reaches a mechanical stop, while the aspirate tubes are able to continue
descending into the wells.
Figure 1 below). The two manifolds move
Dispense
manifold
Figure 1: Patented Design of the 16-Channel Manifold
Tubes are
positioned
for overfill
washing
Aspirate
manifold
Tubes are
positioned
BioTek Instruments, Inc.
Carrier Variations
The carrier is the washer component that transports microplates and positions the
wells underneath the manifold tubes. There are three possible carrier types, and their
usage depends on the manifold type installed and the microplate type being used:
• Microplate carrier that supports 8-, 8s-, and 12-channel manifolds and
solid-bottom plates.
• Microplate carrier that supports 16-, 8-, and 8s-channel manifolds and
solid-bottom plates.
• “Aspiration carrier” that supports 8- and 8s-channel manifolds and 96-well
filter-bottom plates. This carrier is used on ELx50/8FMW model only, and
aspirates fluid from below
through the filters at the bottom of the wells.
The basecode software onboard the ELx50/8FMW has been pre-configured to use
either the microplate carrier or the aspiration carrier. The aspirate-only program
parameters ASPIRATE TYPE and BOT ASPIRATE TIME only appear if the washer
model is the ELx50/8FMW, and the manifold is configured as either 8- or 8s-channel.
ELx50 Washer Variations | 5
the microplates, so that fluid is drawn down
Software Variations
The ELx50™ washer is operated by onboard software, controllable through the
washer’s keypad. This onboard software, referred to as the “basecode,” has an
identifying
Your washer is installed with one of three possible ELx50 basecode part numbers:
4070230, 4070261, or 4070201. Each part number in turn has an identifying
number.
be asked for the part number and version of the basecode currently residing on your
washer. See
information from the washer.
The
4070230 basecode supports:
• Compatibility with the 8-, 8s-, 12, and 16-channel manifolds. The 16-channel
• An easy to use, menu-driven interface.
• Ability to create Wash, Prime, Dispense, Aspirate, Soak, and Link programs for
• Storage of up to 75 washer programs.
• User-definable washer program names, for rapid recall at program run time.
part number.
If you need to contact BioTek Instruments for technical assistance, you will
Product Support & Service, page 12, for instructions for retrieving this
manifold supports both 96- and 384-well plate washing.
automated microplate washing.
version
• Configurable program parameters such as variable soaking, multispeed
shaking, bottom washing, and crosswise aspiration.
• Pre-defined washer programs that can be copied and used as the basis for new
programs.
ELx50 Operator's Manual
6 | Chapter 1: Introduction
• Pre-defined maintenance programs, including AutoPrime, used to keep the
manifold tubes moist and free from clogging.
• Support for the following languages: English, French, German, Italian, and
Spanish.
Note: Contact BioTek for information on changing to a different
language.
• Compatibility with the ELx50/8FMW washer’s aspiration carrier, for bottom
aspiration of 96-well filter-bottom plates. Note: The 16-channel manifold
cannot be used with the aspiration carrier.
• Compatibility with the Liquid Level Alert™ option.
4070261 basecode supports the above features, but it is not compatible with the
The
Liquid Level Alert option and does not support the 16-channel manifold with the
ELx50/8FMW washer’s aspiration carrier.
The
4070201 basecode is not compatible with the 16-channel manifold, the Liquid
Level Alert option, or the ELx50/8FMW washer’s bottom aspiration carrier. This
basecode does not provide the ability to create Soak or Link programs.
BioTek Instruments, Inc.
Hardware Features
• 2-line x 24-character LCD display.
• Membrane keypad with 25 alphanumeric keys.
• Washes:
96-well microplates with 0.355” / 9 mm well centers
384-well microplates with 0.177” / 4.5 mm well centers (washer
model-dependent)
96-well filter-bottom microplates (ELx50™/8FMW model only)
1 x 8 or 1 x 12 microwell strips
• Crosswise aspiration for optimizing washing performance.
• External 24-volt power supply.
• One serial RS-232 COM port (25-pin male connector).
Hardware Features | 7
• Supply and waste systems appropriate for the model/purchased options.
• Internal positive displacement pump provides controllable flow rates and volumes
to make washing cellular assays and immunoassays equally convenient. Settings
are adjustable via the onboard software, for low to high velocity dispensing.
• Internal vacuum pump aspirates liquid from the microwells into the waste
collection vacuum vessel.
• Stepper motors provide accurate and repeatable carrier and manifold positioning.
• Accommodates 1 x 8, 1 x 12, or 1 x 16 manifolds. See
Variations
for more information.
Appendix B, ELx50 Manifold
• Flat, round and ‘V’ bottomed strips and plates are washed.
• Carrier and manifold are easily removed for cleaning.
• Priming/soaking trough built into the carrier.
• Five shaking speeds for more intense washing.
• Programmable aspiration depth: Ensures that the tubes do not touch the well
bottom, allowing the handling of sensitive cell assays.
• ELx50/8FMW model: one vent port (0.047” in diameter) on the aspiration carrier
and two selectable vent plugs (with 0.032” and 0.020” diameter holes) for varying
the amount of vacuum used with the aspiration carrier.
• Mist shield protects the microplate from contaminants.
ELx50 Operator's Manual
8 | Chapter 1: Introduction
Accessories Included
• 24 VDC power supply (PN 61062)
• Power cord (part number varies according to country of use)
• 8-, 8s-, 12-, or 16-channel manifold
• Microplate carrier (specific to manifold type)
• Aspiration carrier (ELx50™/8FMW model only) with two vent plugs:
One plug with 0.032” diameter hole (PN 4072100)
One plug with 0.020” diameter hole (PN 4072099)
• Mist shield (PN 4070517)
• Two 2-liter supply bottles (three with “V” version washers) (PN 4070515)
• One 2-liter waste bottle (PN 48140)
• Waste tubing set:
All models except ELx50/8FMW (PN 4070511)
ELx50/8FMW model only (PN 4073002)
• Supply tubing set (PN 4070510)
• Optional in-line vacuum filter (PN 48146)
• Optional vacuum pump muffler (PN 4073009) for ELx50 washers that are
equipped with the new vacuum pump (washers manufactured after January 2008)
• Stylus maintenance kit (PN 4070523)
• Operator’s Manual (PN 4071000)
• Packing instructions (PN 4071015)
• Set of shipping materials (PN 4073001)
• Shipping document (PN 94075) that includes a Warranty Statement and Certificate
of Compliance and Calibration
• Declaration of Conformity (PN 4071006 for clinical models or PN 4071034 for
non-clinical models)
Part numbers are subject to change over time. Please contact BioTek
Customer Care if you have any questions.
BioTek Instruments, Inc.
Loading...