Biotek BioStack 4, BioStack, BioStack 3, BioStack 2WR Operator's Manual

Operator’s Manual

BioStack

™

Microplate Stacker

BioStack™ Microplate Stacker
Operator's Manual

© 2018
BioTek Instruments, Inc.
PN 7311000 Rev AH

ii | BioStack Operator's Manual

Notices

BioTek® Instruments, Inc.
Highland Park, PO Box 998
Winooski, VT USA 05404-0998

© 2018, BioTek Instruments, Incorporated. No part of this publication may be
reproduced, transcribed, or transmitted in any form or by any means electronic or
mechanical, including photocopying and recording, for any purpose other than the
purchaser's use without the written permission of BioTek Instruments, Inc.

Trademarks

BioTek® is a registered trademark, and BioStack™, 405™ Microplate Washer,
EL406™, ELx405™, Eon™, Epoch™, Gen5™, Liquid Handling Control™,
MicroFill™, MultiFlo™, MultiFlo™ FX, PowerWave™, Precision™, Precision
Power™, Cytation™ and Synergy™ are trademarks of BioTek Instruments, Inc.
Microsoft® and Windows® are registered trademarks of Microsoft Corporation in
the United States and/or other countries.

All other trademarks are the property of their respective holders.

Restrictions and Liabilities

Information in this document is subject to change and does not represent a
commitment by BioTek Instruments, Inc. Changes made to the information in this
document will be incorporated in new editions of the publication. No
responsibility is assumed by BioTek for the use or reliability of software or
equipment that is not supplied by BioTek or its affiliated dealers.

BioStack™ Microplate Stacker

Notices| iii

Contents

Notices ii
Contact Information vi

Global Service and Support vi
Customer Service and Sales vi
Service/Technical Assistance Center (TAC) vi
European Coordination Center/Authorized European Representative vi

Revision History vii
Document Conventions xi

Intended Use Statement xi
Quality Control xii
Warranty and Product Registration xii
Warnings xii

Hazards xiii
Precautions xiv
CE Mark xvi

Directive 2014/30/EU: Electromagnetic Compatibility xvi
Directive 2014/35/EU Low Voltage (Safety) xvi
Directive 2012/19/EU: Waste Electrical and Electronic Equipment xvii
Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use) xvii

Electromagnetic Interference and Susceptibility xvii

USA FCC CLASS A xvii
Canadian Department of Communications Class A xvii
User Safety xviii

Safety Symbols xix

BioStack 3 and BioStack 4: Secure Gripper Before Moving xx

Introduction 1

Introducing the BioStack 2

Product Description 3
BioStack 4: Notes about Handling Lidded Plates 4

Package Contents 6
Optional Accessories 6
Specifications 9

Stay-On Lid Mode (BioStack 4 Only) 10

Installation 13

Upgrade Scenarios 14
Unpack and Inspect the Instrument 15
Setting Up the BioStack 17
Remove the Shipping Hardware—BIOSTACK2WR 18
Remove the Shipping Hardware—BioStack 3 20
Remove the Shipping Hardware—BioStack 4 23

Wrist Angle Setting Requirements 26
0 Degree Orientation for Liquid Handlers 27

Dip Switch Settings 28

Change Setting for Wrist Angle 29
Change Setting for 50-Plate Stacks 30

BioTek Instruments, Inc.

iv | BioStack Operator's Manual

Test Plates for Compatibility—BioStack 4 Only 30

Connect to Power 31

Connect to Computer 32
Connect to Liquid Handler 33

Where to Go Next 35
Repacking the BioStack 36
Install the Shipping Hardware—BioStack2WR 37
Install the Shipping Hardware—BioStack 3 37
Install the Shipping Hardware—BioStack 4 38

Repacking—Install Shipping Panel 39

Operation 41

Before You Start 42
Load Plates into Input Stack 43

Load Plates Using the Pedestal 44
Supported Microplate Types and Dimensions 45

Processing Microplates 46

Controlling the BioStack: 46
Stopping Plate Processing 47
Restack the Plates 49

Processing Plates with Lids 49

Plate De-Lidding: How It Works 50
Prevent Condensation on Plate Lids 51
Plate Lid Definition Files 52
Microplate and Lid Dimensions 53
Plate Lid Advanced Dimensions 54
Start Up the BioStack 57

Maintenance 59

Overview 60
Recommended Maintenance Schedule 60
Warnings and Precautions 61
Required Materials 61
Routine Cleaning Procedure 63
Decontamination 64

Tools and Supplies 65
Decontaminate the BioStack 65

Cleaning and Lubricating the Linear Ways 66

Qualification 69

Overview 70
Recommended Qualification Schedule 70
Installation Qualification 72
Operational Qualification Procedure 72

Troubleshooting & Error Codes 73

Troubleshooting 74
Communication Error Codes 74

BioStack Instrument Errors 76
Plate Lid Definition Errors 82

BioStack™ Microplate Stacker

Notices| v

Washer or Dispenser Communication Error Codes 85
BioStack Sensor Numbers 86
BioStack Motor Numbers 87
Interfacing Instrument Numbers 87
Plate Stacked Height 88
Change Setting for Low-Profile Plates 89
Loosen Plate Carrier Set Screws - BioStack 4 Only 90
Adjust the Stack Dogs 91

Install the Barcode Scanner 93

Barcode Scanner Overview 94
Install the Barcode Scanner 94

Change Dip Switch Setting for Barcode Scanner 97
Install the Scanner Cable Duct 97

Preparing and Attaching Barcode Labels 99
Scanner Test with Readers 101

To Report Barcodes with Reader Results 101

Scanner Test with Precision/XS 103

To Report Scanned Codes with Precision/XS 104

BioTek Instruments, Inc.

vi | BioStack Operator's Manual

Contact Information

BioTek Instruments, Inc.

Highland Park, P.O. Box 998

Winooski, Vermont 05404-0998 USA

Global Service and Support

BioTek instrument service and repair is available worldwide at several of BioTek’s
International Service Centers and in the field at your location. To arrange for service
or repair, contact the office nearest you; visit www.biotek.com for up-to-date
contact information. For customer service, sales, and technical assistance, refer to
the information below.

Customer Service and Sales

Internet: www.biotek.com

Phone:

 802-655-4740(outsidetheUS)

Fax: [phone]

Email: [e-mail]

888-451-5171 (toll-free in the US)

Service/Technical Assistance Center (TAC)

Phone:

 802-655-4740(outsidetheUS)

Fax: [phone]

Email: [e-mail]

800-242-4685 (toll-free in the US)

European Coordination Center/Authorized European Representative

BioTek Instruments GmbH

Kocherwaldstrasse 34

D-74177 Bad Friedrichshall

Germany

Internet: www.biotek.de

BioStack™ Microplate Stacker

Revision History| vii

Phone:

Fax: +49(0)7136968111

Email: [e-mail]

[phone]

Revision History

Rev Date Changes

A 11/2002 First issue.

B 5/2003 Throughout: Clarified the compatibility of the ELx405 with the BioStack by

specifying the Washer’s Auto Plate and Select models only. Updated
contact information, Safety Symbols, and Warranty.

C 10/2003 Preface: Updated the Intended Use statement to distinguish between the

European Union and all other jurisdictions.

D 06/2004 Throughout: Reformatted manual for cosmetic effect. Added references

about compatibility of the BioStack with the ELx405 HT and ELx405 Select
CW Microplate Washers. Added new sections about compatibility,
installation, operation, alignment, etc., of the Precision and Precision XS.

Revised the Technical Support section and added new sections on Depot

Service Contracts and Applications Support. Enhanced Introduction,
Installation, Operation, and Maintenance chapters.

E 06/2005 Throughout: Added information on the new barcode scanner and USB port.

Changed ELx405 “Auto” model to “ELx405.”

Moved instructions from former Appendix B, Serial Cable Connections and

Appendix C, Mounting the Aligning Plates and Posts into Chapter 3,
Installation.

Chapter 5: Simplified/clarified IQ and OQ sections. Chapter 7: Added KC4

error codes for BioStack operation with readers, and BioStack barcode
scanner error codes.

F 05/2006 Added instructions for using Gen5 software. Preface: Updated safety

information and removed Warranty, Registration Card, and Registration
Online sections in Preface. Added laser beam warning. Appendix B: Added
laser beam warning.

G 07/2006 Added instructions for installing/operating the BioStack with the Synergy 2.

H 12/2006 Added instructions for installing and operating the BioStack with the

NanoQuot. In Chapter 5 and Appendix B: Added the Barcode Scanner Test
as an optional IQ and OQ test.

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viii | BioStack Operator's Manual

Rev Date Changes

I 06/2007 Added instructions for installing and operating the BioStack with the

Synergy 4. Updated error code information. Incorporated manual update
revision H1 as Appendix C, Reconfiguration of the BioStack. Added
Appendix D, Required Software Versions.

J 08/2007 Added references to the Liquid Handling Control (LHC) Software throughout

the manual, and instructions for installation/operation of the software for
PC control of the BioStack and ELx405.

K 10/2007 Added instructions for installing and operating the BioStack with the

MicroFlo Select.

L 03/2008 Added instructions for installing and operating the BioStack with the

PowerWave XS2. Changed “μFill” to “MicroFill.”

M 06/2008 Changed “Bio-Stack” to “BioStack.” Added references to the fixed wrist

model (PN BIOSTACK2) and rotational wrist model (PN BIOSTACK2WR).
Added instructions for installation/operation of the new models with
interfacing instruments.

N 10/2008 Added instructions for installing and operating the BioStack with the EL406.

Changed “Alignment Kit” to “Integration Kit” throughout. Added more
information about installation of two BioStacks with two interfacing
instruments (ELx405, MicroFlo Select, EL406).

O 12/2008 Added instructions for installing and operating the BioStack with the

Synergy Mx. Removed references to obsolete BioStack PN BIOSTACK.

P 04/2009 Added information regarding instrument control of the EL406 and

installation/operation instructions for controlling the BioStack and EL406
via the keypad. Added more information about how the BioStack barcode
scanner works in Appendix B. Added instructions or notes throughout the
manual instructing the user to turn the BioStack on before the interfacing
instrument, and to manually raise the BioStack’s claw/gripper above the
interfacing instrument’s plate carrier or supply station (Precision) before
turning the BioStack on.

Q 01/2010 Added instructions for installing and operating the BioStack with the

Synergy H4 reader.

R 09/2010 Overall: Streamlined and rearchitected content to enhance usability and

support maintenance and reuse. Moved installation and operation
instructions, software version compatibility tables, software data sheets,
and information on obtaining software version information out of the main
Operator’s Manual and into individual, instrument-specific PDF files on the
BioStack Operator’s Manual CD. Added support for the MultiFlo Dispenser.

Preface: Updated Intended Use Statement; Warnings, Hazards, and

BioStack™ Microplate Stacker

Revision History| ix

Rev Date Changes

Precautions; Directives; and Safety Symbols.

Chapter 1 Introduction: Updated Package Contents and Optional

Accessories lists. Chapter 2 Installation: Added “Configure for 50-Plate
Stacks.” Chapter 5 Instrument Qualification: Removed IQ Checklists.
Moved the Software Data Sheets to the individual instrument-specific PDF
files. Chapter 6 Error Codes: Removed error code tables for MicroFlo and
EL406 Interface Software, and Gen5/KC4 Function Call Errors.

S 11/2010 Added instructions for installing and operating the BioStack with the

Synergy H1 reader. Emphasized the importance of orienting microplates in
the input stack to match the expected placement of well A1 on the
interfacing instrument’s plate carrier.

T 8/2011 Added instructions for installing/operating the BioStack with the Eon,

Epoch.

U 3/2012 Added instructions for installing and operating the BioStack with the 405

TS. Added references to the 10-plate stacks.

V 6/2012 Added support for BIOSTACK3WR (for liquid handlers). Removed

references to BioStack2. Added ‘Service’ and ‘Accessories’ hazard
warnings. Added ‘Spare Parts’ precaution. Changed all references from
claw/gripper to gripper. Added Wrist Angle Setting Requirement and Dip
switch Settings content. Revised descriptions of the microplate
requirements. Replaced former Appendix B, Microplate Stack
Adjustment/Configuration for Half-Height Plates with section in
Troubleshooting chapter: Adjust Stack Dogs. Updated error codes and
added Troubleshooting guidelines to the same chapter. Omitted former
Appendix C, Information for External Developers, as it was obsolete.
Updated format and text styles, replaced some drawings with photos.
Revised the liquid handler instrument-specific sections; conversely, the
reader instrument-specific PDFs are unchanged since the last revision. The
Nanoquot instrument-specific section has been omitted.

W 7/2012 Added support for BioStack3 for readers and for controlling both BioStack

models using Gen5 alone (i.e. BioStack PC Control Software is no longer
needed for the readers). Consolidated the Integration Kits when applicable,
i.e. universal aligning base plates support both BioStack models. Removed
references to obsolete aligning base plate in instrument-specific
instructions. Added units to the barcode label graphic.

X 4/2013 Added instructions for installing and operating the BioStack with the

Cytation 3. Corrected or improved content based on suggestions from field
staff, including deleting the statement that barcode labels can be purchased
from BioTek. Added references to the rubber band that secures the
BioStack3's wrist during shipping. Recommended using rubber bands to

BioTek Instruments, Inc.

x | BioStack Operator's Manual

Rev Date Changes

secure gripper before moving the BioStack 3.

Y 11/2013 Added instructions for installing and operating the BioStack with the

MultiFlo FX. Added Precision Universal models (PRC384U) to the list of
instruments that are not BioStack-compatible. Restored references to, and
added a diagram for 0° integration for liquid handlers and the
BIOSTACK2WR. Added "Space Requirement Dimensions" for most
interfacing instruments.

Z 12/2013 Introduced BioStack 4 and its support of plate lids during processing. Added

hazard label and description: cutting fingers/hand. Added "Plate
Compatibility Test" for BioStack4 only. Added support for the Synergy Neo

- BioStack4 integration.

AA 6/2014 Added support for new instruments: Epoch2 and Synergy HTX. Added a

warning that the PowerWave HT is not compatible with the BioStack 4 and a
"strong recommendation" to measure the plate and lid type before use to
verify they meet the minimum requirements for de-lidding operations with
the BioStack 4.

AB 9/2014 Updated specifications to include certain low-density plates with lids that

are supported in "stay-on lid" mode. Added references to stay-on lid mode
where applicable. Corrected the space requirement for liquid handlers:
increased the depth required by six inches to account for wrist movement.

AC 12/2014 Added support for Cytation 5. Updated the "Stay-on Lid" mode description

to describe the newly relabeled stacks required to support this feature, i.e.,
Tall Lidded Plate Stacks. Updated notice of compliance with EU Directives.

AD 2/2015 Updated Synergy Neo integration instructions to match new and improved

alignment hardware. Added new 28 volt power supply to parts list.

AE 3/2015 Reverted contents to match revision AC.

AF 7/2015 Added support for Synergy Neo2, updated Contact Information, changed

RMA to Service Call Notice (SCN) number, updated Synergy Neo and
Synergy Neo2 integration kit to PN 7310055

AG 2017 Updated PN for replacement shipping materials 7313002 to 1163003;

removed reference to the ANSI standard in the microplate specification sec­tion; add note to “Install and Run BioStack with the Synergy H1” to point
out position of bracket on alignment plate, which varies depending on
which BioStack is used; added Cytation 1 support; resolved EPR 24692 by
adding error code 2002 to the list; modified instructions for installing soft­ware and testing communication in the Install and Operate the BioStack
with Precision document; added USB and serial cable part numbers to the
fluid interfacing instrument documents.

BioStack™ Microplate Stacker

Document Conventions| xi

Rev Date Changes

AH 12/2018 Updated Epoch 2 information to correspond with the updated Epoch 2 instru-

ment; corrected alignment plate PN for Synergy H1; updated the drawing
of the Cytation showing the space needed for an instrument with a Cooling
Accessory installed; updated PN for BioStack2 shipping container to PN
7310014; updated BioStack leg PN for use with Epoch 2 to 7310570;
updated the PN for the baseplate for the Microflo FX to 7312138; added
error code 020D.

Document Conventions

This manual uses the following typographic conventions:

n This note format calls attention to important information.

Warnings are presented in this style to call attention to potential hazards and

other safety concerns.



This icon calls attention to important safety information.

Tips and suggestions for improving performance are formatted this way.

Intended Use Statement

The BioStack Microplate Stacker is an automated microplate stacking system that is
designed to systematically transfer a stack of microplates to and from compatible
BioTek laboratory instruments wherever washing, dispensing, pipetting, or reading
of multiple microplates is performed.

If the instrument has an “IVD” label it may be used for clinical and non-clinical
purposes, including research & development. If there is no such label the
instrument may only be used for research & development or other non-clinical
purposes.

BioTek Instruments, Inc.

xii | BioStack Operator's Manual

Quality Control

It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for
the test to be conducted. Failure to conduct Quality Control checks could result in
erroneous test data.

Warranty and Product Registration

Please take a moment to review the Warranty information that shipped with your
product. Please also register your product with BioTek to ensure that you receive
important information and updates about the product(s) you have purchased.

You can register online through BioTek’s Customer Resource Center (CRC) at

www.biotek.com or by calling 888/451-5171 or 802/655-4740.

Warnings

Operate the instrument on a level, stable surface away from excessive
humidity.
When operated in a safe environment, according to the instructions in this
manual, there are no known hazards associated with the BioStack. However,
the operator should be aware of certain situations that could result in serious
injury: see Hazards and Precautions.

BioStack™ Microplate Stacker

Hazards

The following hazard warnings are provided to help avoid injury:

Warning! Power Rating. The instrument’s power supply or power cord must

be connected to a power receptacle that provides voltage and current within the
specified rating for the system. Use of an incompatible power receptacle may
produce electrical shock and fire hazards.

Hazards| xiii





Warning! Electrical Grounding. Never use a plug adapter to connect primary

power to the external power supply. Use of an adapter disconnects the utility
ground, creating a severe shock hazard. Always connect the power cord directly to
an appropriate receptacle with a functional ground.

Warning! Service. Only qualified technical personnel should perform service

procedures on internal components.

Warning! Accessories. Only accessories which meet the manufacturer’s

specifications shall be used with the instrument.

Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into

internal components creates a potential for shock hazard or instrument damage. If a
spill occurs while a program is running, abort the program and turn the instrument
off. Wipe up all spills immediately. Do not operate the instrument if internal
components have been exposed to fluid.

Warning! Unspecified Use. Failure to operate this equipment according to the

guidelines and safeguards specified in this manual could result in a hazardous
condition.

Warning! Software Quality Control. The operator must follow the

manufacturer’s assay package insert when modifying software parameters and
establishing reading, washing, or dispensing methods. Failure to conduct quality
control checks could result in erroneous test data.

Warning! Internal Voltage. Always turn off the power switch and unplug the

power supply before cleaning the outer surface of the instrument.

Warning! Potential Biohazards. Some assays or specimens may pose a

biohazard. Adequate safety precautions should be taken as outlined in the assay’s
package insert. This hazard is noted by the symbol shown here. Always wear safety
glasses and appropriate protective equipment, such as chemically resistant rubber
gloves and apron.

Warning! Laser Beam. Serious eye injury may occur if you stare directly into

the laser beam of the barcode scanner during operation of the scanner. This hazard
is noted by the symbol shown here. Do not look directly into the laser beam during
operation of the scanner.





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xiv | BioStack Operator's Manual

Warning! Pinch Hazard. Some areas of the instrument or its components can

present pinch hazards when the instrument is operating. These areas are marked
with the symbol shown here. Keep hands/fingers clear of these areas when the
instrument is operating.

Warning! Cutting of Fingers or Hand Hazard. The metal plate gripper

presents a cutting hazard to fingers/hands when the instrument is operating. The
gripper is marked with the symbol shown here. Keep fingers/hands away from the
gripper when the instrument is operating.

Precautions

The following precautions are provided to help avoid damage to the instrument(s):

Caution: Service. The instrument should be serviced by BioTek-authorized

service personnel. Only qualified technical personnel should perform troubleshooting
and service procedures on internal components.

Caution: Spare Parts. Only approved spare parts should be used for

maintenance. The use of unapproved spare parts and accessories may result in a
loss of warranty and potentially impair instrument performance or cause damage to
the instrument.

Caution: Environmental Conditions. Do not expose the instrument to

temperature extremes. For proper operation, temperaturess near the instrument
should remain within the range listed in the Specifications section. Performance
may be adversely affected if temperatures fluctuate above or below this range.

Caution: Sodium Hypochlorite. Do not expose any part of the instrument to

the recommended diluted sodium hypochlorite solution (bleach) for more than 20
minutes. Prolonged contact may damage the instrument surfaces. Be certain to
rinse and thoroughly wipe all surfaces.

Caution: Power Supply. Only use the power supply shipped with the

instrument. Operate this power supply within the range of line voltages listed on it.

Caution: Disposal. Dispose of the instrument according to Directive

2002/96/EC, “on waste electrical and electronic equipment (WEEE),” or local
ordinances.

Caution: Warranty. Failure to follow preventive maintenance protocols may

void the warranty.

Caution: Shipping Hardware. All shipping hardware (e.g., shipping panel,

shipping block, carrier shipping screws, etc.) must be removed before operating the
instrument and reinstalled before repackaging the instrument for shipment.

Caution: Waste Sensor Port (for customers who have purchased the

BioStack for use with the ELx405, 405 TS/LS, or EL406). The waste sensor port on
the back of the washer/dispenser is the same type as the 24-VDC power connector
on the back of the BioStack. Do not plug the BioStack’s external power supply into

BioStack™ Microplate Stacker

Precautions| xv

the washer’s/dispenser’s port; it will permanently damage internal components.

Caution: Aligning Posts. When installing the BioStack’s four aligning posts,

use caution not to cross thread these parts. Finger-tighten only!

Caution: BioStack Barcode Scanner Mirror. Do not scratch or damage

the mirror when unpacking or installing the barcode scanner.

Caution: Electromagnetic Environment. Per IEC 61326-2-6 it is the user’s

responsibility to ensure that a compatible electromagnetic environment for this
instrument is provided and maintained in order that the device will perform as
intended.

Caution: Electromagnetic Compatibility. Do not use this device in close

proximity to sources of strong electromagnetic radiation (e.g., unshielded
intentional RF sources), because these may interfere with the proper operation.

BioTek Instruments, Inc.

xvi | BioStack Operator's Manual

CE Mark

Based on the testing described below and information contained
herein, this instrument bears the CE mark.

n Note: Refer to the Declaration of Conformity for specific details.

Directive 2014/30/EU: Electromagnetic Compatibility

Emissions—Class A

The system has been type-tested by an independent, accredited testing
laboratory and found to meet the requirements of EN 61326-1: Class A for
Radiated Emissions and Line Conducted Emissions.

Verification of compliance was conducted to the limits and methods of EN 55011
(CISPR 11) Class A. In a domestic environment it may cause radio interference,
in which case, you may need to take measures to mitigate the interference.

Immunity

The system has been type-tested by an independent, accredited testing
laboratory and found to meet the requirements of EN 61326-1 and EN 61326-2-6
for Immunity. Verification of compliance was conducted to the limits and
methods of the following:

EN 61000-4-2, Electrostatic Discharge
EN 61000-4-3, Radiated EM Fields
EN 61000-4-4, Electrical Fast Transient/Burst
EN 61000-4-5, Surge Immunity
EN 61000-4-6, Conducted Disturbances from RFI
EN 61000-4-11, Voltage Dips, Short Interruptions and Variations

Directive 2014/35/EU Low Voltage (Safety)

The system has been type-tested by an independent testing laboratory and was
found to meet the requirements of this Directive. Verification of compliance was
conducted to the limits and methods of the following:

EN 61010-1, “Safety requirement for electrical equipment for measurement,

control and laboratory use. Part 1, General requirements.”

EN 61010-2-081, “Particular requirements for automatic and semi-automatic

laboratory equipment for analysis and other purposes.”

BioStack™ Microplate Stacker

Electromagnetic Interference and Susceptibility| xvii

Directive 2012/19/EU: Waste Electrical and Electronic Equipment

Disposal Notice: Dispose of the instrument according to Directive 2012/19/EU,
“on waste electrical and electronic equipment (WEEE)” or local ordinances.

Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use)

n Product registration with competent authorities

n Traceability to the U.S. National Institute of Standards and Technology (NIST).

EN 61010-2-101 Particular requirements for in vitro diagnostic (IVD) medical
equipment.

Electromagnetic Interference and Susceptibility

USA FCC CLASS A

RADIO AND TELEVISION INTERFERENCE

NOTE: This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits
are designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if not installed
and used in accordance with the instruction manual, may cause harmful
interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference, in which case the user
will be required to correct the interference at their own expense.

In order to maintain compliance with FCC regulations shielded cables must be
used with this equipment. Operation with non-approved equipment or
unshielded cables is likely to result in interference to radio and television
reception.

Canadian Department of Communications Class A

This digital apparatus does not exceed Class A limits for radio emissions from
digital apparatus set out in the Radio Interference Regulations of the Canadian
Department of Communications.

Le present appareil numerique n'émet pas de bruits radioelectriques depassant
les limites applicables aux appareils numerique de la Class A prescrites dans
le Reglement sur le brouillage radioelectrique edicte par le ministere des
Communications du Canada.

BioTek Instruments, Inc.

xviii | BioStack Operator's Manual

User Safety

This device has been type-tested by an independent laboratory and found to meet
the requirements of the following:

l Underwriters Laboratories UL 61010-1 “Safety requirements for electrical

equipment for measurement, control and laboratory use; Part 1: general
requirements.”

l Canadian Standards Association CAN/CSA C22.2 No. 61010-1 “Safety

requirements for electrical equipment for measurement, control and
laboratory use; Part 1: general requirements.”

l EN 61010 Standards. See CE Mark on page xvi.

BioStack™ Microplate Stacker

Safety Symbols

Some of these symbols appear on the instrument or accessories:

Alternating current
Courant alternatif
Wechselstrom
Corrientealterna
Correntealternata

Safety Symbols| xix

Both direct and alternating
current
Courant continu et courant
alternatif
Gleich - und Wechselstrom
Corriente continua y
corrientealterna
Corrente continua e
correntealternata

Direct current
Courant continu
Gleichstrom
Corriente continua
Corrente continua

On (Supply)
Marche (alimentation)
Ein (VerbindungmitdemNetz)
Conectado
Chiuso

Off (Supply)
Arrêt (alimentation)
Aus (TrennungvomNetz)
Desconectado
Aperto
(sconnessionedallaretedialimentazi
one)

Warning, risk of electric shock
Attention, risque de choc électrique
Gefährlicheelektrischeschlag
Precaución, riesgo de
sacudidaeléctrica
Attenzione, rischiodiscossaelettrica

Earth ground terminal
Borne de terre
Erde (Betriebserde)
Borne de tierra
Terra (difunzionamento)

Protective conductor terminal
Borne de terre de protection
Schutzleiteranschluss
Borne de tierra de protección
Terra diprotezione

Caution (refer to
accompanying documents)
Attention (voir documents
d’accompanement)
AchtungsieheBegleitpapiere
Atención (vease los
documentosincluidos)
Attenzione, consultare la doc
annessa

Warning, risk of crushing or
pinching
Attention, risqued’écrasement
et pincement
Warnen, Gefahr des
Zerquetschens und Klemmen
Precaución, riesgo del
machacamiento y sejeción
Attenzione,
rischiodischiacciareedintrappol
arsi

Warning, hot surface
Attention, surface chaude
Warnen, heißeOberfläche
Precaución, superficiecaliente
Attenzione, superficiecalda

Warning, potential biohazards
Attention,
risquesbiologiquespotentiels
Warnung!
MoeglichebiologischeGiftstoffe
Atención, riesgosbiológicos
Attenzione, rischiobiologico

BioTek Instruments, Inc.

xx | BioStack Operator's Manual

In vitro diagnostic medical device
Dispositif médical de diagnostic in
vitro
Medizinisches In-Vitro­Diagnostikum
Dispositivo médico de diagnóstico in
vitro
Dispositivo medico diagnostico in
vitro

Consult instructions for use
Consulter la notice d’emploi
Gebrauchsanweisung beachten
Consultar las instrucciones de uso
Consultare le istruzioni per uso

Separate collection for
electrical and electronic
equipment
Les équipements électriques
et électroniques font l’objet
d’une collecte sélective
Getrennte Sammlung von
Elektro- und
Elektronikgeräten
Recogida selectiva de aparatos
eléctricos y electrónicos
Raccolta separata delle
apparecchiature elettriche ed
elettroniche

Laser radiation: Do not stare
into beam
Rayonnement laser: Ne pas
regarder dans le faisceau
Laserstrahlung: Nicht in den
strahl blicken
Radiación de láser: No mire
fijamente al rayo
Radiazione di laser: Non stare
nel fascio

BioStack 3 and BioStack 4: Secure Gripper Before Moving

Note: Do not let the gripper flop around! Before moving the BioStack 3 or BioStack 4,

secure the gripper with a rubber band or tape to prevent it from losing its alignment. A
complex recalibration is required if it is knocked out of position.

BioStack™ Microplate Stacker

Chapter 1

Introduction

Thank you for purchasing the BioStack™ Microplate Stacker.
This chapter describes its features and specifications and
includes important contact information.

Introducing the BioStack 2
Package Contents 6
Optional Accessories 6
Specifications 9

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2 | Chapter 1: Introduction

Introducing the BioStack

This manual covers the BioStack (BIOSTACK2WR), BioStack3 (BIOSTACK3WR),
and BioStack4 (BIOSTACK4). Unless otherwise specified, the term BioStack refers
to all models.

BioStack 3 shown without stacks

Side view

1. Gripper—arm with wrist and gripper

2. Plate carrier Back view

3. Stack locks 1. Power switch

4. Dip switch access 2. Power cable port

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3. LED status indicator

4. Barcode scanner port

5. Computer and interfacing
instrument ports

BioStack™ Microplate Stacker

Introducing the BioStack | 3

BioStack Gripper arm Stack, pedestal and plates

All current BioStack models have rotational wrists that
enable them to interface with most BioTek instruments in
the most convenient and comfortable angle/position.

A tool to help load plates into
the stack, called a pedestal, is
provided with the BioStack: See

Load Plates Using the
Pedestal on page 44.

Important: Review the Wrist Angle Setting Requirements on page 26 to determine

if you need to change the wrist angle setting to interface correctly with your BioTek reader
or liquid handler.

Product Description

The BioStack Microplate Stacker is a simple and cost-effective solution for benchtop
automation of BioTek’s microplate instrumentation. The BioStack's compatibility
with a range of detection and liquid handling systems provides laboratories high
throughput processing for a variety of applications in genomics, proteomics, drug
discovery, and clinical diagnostics. Speed, ease of use, and walk-away automation
are guaranteed for any routine microplate process.

The BioStack’s small footprint fits easily in standard laboratory hoods and may not
require a computer. As a batch processor for BioTek readers and liquid handlers,
the BioStack interfaces seamlessly with BioTek’s Gen5 Data Analysis Software and
Liquid Handling Control (LHC) Software.

Up to two BioStacks (2WR models only) can interface with the Precision Microplate
Pipetting System for unattended plate replication, bulk reagent dispensing, or other
liquid handling operations.

BioTek Instruments, Inc.

4 | Chapter 1: Introduction

Features:

l Automates routine laboratory procedures

l 10, 30, or 50 standard height microplates per interchangeable stack

l Simple to use for all laboratory personnel

l Restacking capability

l Small footprint conserves precious benchtop space

l Universal design compatible with all supported BioTek instrumentation

l Designed for heavy use with aluminum precision-ground base and synchronistic

movement

l Linear mechanisms built around proven BioTek technology

l Quiet operation with cams activated by stepper motors

l Reliable transport with microplate gripped from below during transfers

l Onboard self-diagnostics ensures trouble-free operation

l BioStack 4 Only: Handles microplates with lids: removes the lid before plate transfer to

the attached instrument, and replaces the lid after retrieval from the instrument,
except in "stay-on lid" mode.

Models:

l PN BIOSTACK2WR: Supports 90° and 0° integrations; one plate at a time transfer.

l PN BIOSTACK3WR and BIOSTACK4: Support +90°, -90°, and 0° integrations; two

plates in motion for every transfer. BioStack 4 processes plates with or without lids.

BioStack 4: Notes about Handling Lidded Plates

The BioStack 4 functions similarly and can do everything the other BioStack models
do, and it can process microplates with lids.

l The required stacks are labeled "TALL LIDDED PLATE STACK" to process plates with

lids.

l The BioStack 4 supports standard stack types when not handling lids. (Conversely, do

not use the "tall lidded plate stacks" with BioStack 2/3 models.)

l The "tall lidded plate stack" also supports taller plates without lids; it can handle plates

up to 22 mm. (Maximum supported height of plate + lid = 23.2 mm when Stay-On Lid

Mode requirements are met.)

l Standard stacks are recommended for handling low-profile PCR plates. The BioStack 4

has been designed to better handle these plates that warp easily, but the best
performance is achieved combining the BioStack 4 with the standard stacks.

l Only 30- and 10-plate stacks are supported when processing lids. And, the stacks hold

fewer plates when lids are included: the 30-plate stack holds about 25 plates with lids
and the 10-plate stack holds approximately 8 plates with lids, depending on the plate

BioStack™ Microplate Stacker

Introducing the BioStack | 5

and lid type and manufacturer. Another limiting factor is the total weight of the filled
plates in the stack cannot exceed 7.0 lbs (3.2 kg).

l When processing plates with lids the BioStack 4 performs several extra error-checking

movements, using its fingers to determine if the expected labware, i.e., a plate, lid, or
combination, is present. You'll observe several extra finger movements during
processing.

l

Except in Stay-On Lid Mode, lids are always
removed from the plates during processing even when the option to "Keep lids on
during read" is selected. In this case, the lid is removed briefly and replaced almost
immediately. Lid integrity is maintained: the lid removed from a plate is always the
same lid restored to the plate.

l A Plate Lid Definition file is required to process plates with lids.

n Gen5: Lid dimensions are stored in the Plate Type Database record. BioTek

updated the most commonly used plate type records with lid dimensions and
users can add more, as needed.

n LHC and touch screen liquid handlers: Several plate lid definition files are

provided to support the most common types of plates and new files can be
created. Important: A copy of the Plate Lid Definition file is attached to the
protocol for processing. The protocol no longer references the original lid
definition file, only its copy. If the original plate-lid definition file is modified,
those changes are not applied to the copies of the file attached to protocols.

n Keypad Liquid Handlers: Must use LHC to process plates with lids. Keypad

instruments can control the BioStack 4 when not processing plates with lids,
identical to BioStack 3 behavior.

l Certain plate types are not compatible with the de-lidding operation. Measure your

plate and lid type before use to verify they meet the minimum requirements:
Specifications on page 9. The BD Falcon 351172, for example, is a nonsupported
plate type.

BioTek Instruments, Inc.

6 | Chapter 1: Introduction

Package Contents

n Part numbers and package contents are subject to change and vary according

to instrument model. Please contact BioTek Customer Care if you have any
questions.

n BioStack Microplate Stacker (PN BIOSTACK2WR, BIOSTACK3WR, BIOSTACK4)

n Power supply: 24 volt (PN 01281)

n Plate stacking pedestal (PN 7312083)

n Grease kit (PN 7110017)

n BioStack Operator’s Manual (PN 7311000) on USB flash drive (PN 7310571)

Optional Accessories

l Set of two microplate stacks

Stack Type Any BioStack

¥

BioStack 4*

30-plate 7310008‡ 1230004

10-plate 7310030 1230003

50-plate 7310539 N/A

* These stacks are required for transferring plates with lids. The 50-plate stacks can be used on the
BioStack 4 to process plates only, not plates with lids.
¥ Recommended for low-volume PCR plates.
‡ Also supports up to 44 low-profile, 1536-well plates without lids.

Note: Stack Types are named for the number of plates they can hold without lids.
When using lids, the approximate number of plates with lids the 30-plate stack holds is 25;
for the 10-plate stack, it's 8, depending on the manufacturer. Another limiting factor is
that the total weight of the filled plates in the stack cannot exceed 7.0 lbs (3.2 kg).

BioStack™ Microplate Stacker

Optional Accessories | 7

Microplate Stacks with height in inches: 50-, 30-, and 10-plate

l Instrument Qualification package (PN 7310530)

l Barcode scanner and installation kit (PN 7310017)

l Integration Kits for use with interfacing instruments:

Instrument Integration Kit

405 TS/LS PN 7310040

Cytation 1/3/5 PN 7310053

EL406 PN 7310042

ELx405 PN 7310041

Eon PN 7310049

EpochR BIOSTACK2WR: PN 7310032

BIOSTACK3WR/BIOSTACK4: PN 7310043

Epoch 2 PN 7310054

MicroFill* PN 7310012

BioTek Instruments, Inc.

8 | Chapter 1: Introduction

Instrument Integration Kit

MicroFlo Select* PN 7310020

MultiFlo/MultiFlo FX PN 7310044

PowerWave HT

¥

PN 7310048

¥

Precision, Precision XS* PN 7110004

Synergy HT/HTX PN 7310046

Synergy 2/4/Mx/H4 PN 7310047

Synergy H1 PN 7310045

Synergy Neo and Synergy

PN 7310055

Neo2‡

*Compatible with BIOSTACK2WR only.

‡

Compatible with BioStack 4 only and uses its own barcode scanner, i.e., not compatible with the BioStack

barcode scanner listed above.

¥

Not compatible with BIOSTACK4.

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BioStack™ Microplate Stacker