Biotage PRESSURE+ 48 User Manual

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Biotage® PRESSURE+ 

User Manual

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Contents

Biotage® PRESSURE+ 

User Manual

CONTENTS

 Safety

1 Intended Use 1 Education, Training, and Competence 1 Warranty and Liability 1 Service 1 Equipment Safety Standards 1 Labels 1 Safety Requirements

 Installation

2 Site Requirements 2 Unpack and Install

 System Description

4 Load the Columns 5 Flow Control

 Operation

6 Step-by-Step Instructions

 Maintenance

8 Replace the Sealing Gasket 8 Clean

 General Information

9 Consumables and Accessories 9 Manufacturer 9 Contact Us

 Biotage® PRESSURE+ 48 User Manual | © Biotage 2017

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Safety

Intended Use

Biotage PRESSURE+ 48 is intended for laboratory use only and has to be operated by trained professionals. All operations must be performed:

According to the user documentation delivered with the equipment.

According to instructions available at www.biotage.com.

According to instructions given by the technical suppor t staf f from Biotage.

Within limits set by the technical specification.

Failure to follow these instructions and operate within the limits set by the technical specification may result in personal injur y and/or equipment damage.

Education, Training, and Competence

It is your responsibility to provide all applicable health and safety regulations to your personnel. You must also ensure that all personnel involved in the operation and maintenance of the equipment fulfill the following criteria:

Have the necessary education, training, and competence required for the intended use of the equipment.

Observe general and specific safety regulations for the use of the equipment and its accessories and consumables at all time.

Warranty and Liability

See the “Biotage Terms & Conditions of Sale” document at www.biotage.com.

Service

All service or adjustments must be performed by an authorized Biotage service engineer. Before handing over the unit for service, it should be cleaned from harmful residues as described in the Clean section on page 8.

It is the responsibility of the customer to inform Biotage Support hazardous biological, radioactive, or toxic samples and/or solvents, prior to any service being performed. When returning equipment to Biotage, this should be done in accordance with the material return procedures supplied separately by Biotage.

™

representatives if the equipment has been used with

1-Point

Equipment Safety Standards

PRESSURE+ 48 and its instruction materials are certified by Biotage to comply with:

CE-Marketing Directive 93/68/EEC

Machinery Directive 2006/42/EC

Labels

Labels used on PRESSURE+ 48:

In accor dance with all the essential requirements of all applicable European product directives; see “Equipment Safety Standards” above.

Consult accompanying user documentation.

Ma nuf a ctu r er.

Safety Requirements

Always keep hands, loose clothing, and long hair away from the manifold when it is moving. Note that slight movement may occur as the unit pressurizes, i.e. when connecting the gas supply.

Many chemicals and samples used with PRESSURE+ 48 can pose chemical, biological, and radiological hazards. You must always understand the potentially hazardous effects of all the materials you work with. To prevent personal injury and/or equipment damage, you must always use impermeable gloves, chemical safety goggles, and protective clothing, follow all generally-accepted lab safety procedures and applicable laws and regulations, and consult your company’s safety expert for guidance.

PRESSURE+ 48 units are manufactured from aluminum, stainless steel, and plastics. Always clean up spills or overflow immediately.

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Installation

Site Requirements

The PRESSURE+ 48 unit should be placed in a laboratory and operated where there is proper ventilation or from inside a chemical fume hood when volatile solvents are used. The PRESSURE+ 48 unit requires a compressed gas supply, and should be located with this in mind.

Table/bench Flat, st able, and able to support the weight of

Operating temperature

Humidity Maximum relative humidity of 80% for

Altitude Up to 2000 meters

Gas Source

PRESSURE+ 48 operates using a compressed gas both to seal the columns and displace liquid. Whether compressed air or nitrogen is used, or cylinders of purified air or nitrogen, the gas should be free of moisture, particulates, and hydrocarbons. This is essential to prevent sample contamination and general fouling of the manifold.

the unit (14 kg/31 lbs).

The dimensions of the unit (W x D x H) is 335 x 305 x 390 mm (13.2" x 12.0" x 15.4"). The free space above and behind the bench should be at least 10 cm (4") for ventilation.

5°C to 40°C (41°F to 104°F), indoor use only

temperatures up to 31°C (87.8°F), decreasing linearly to 50% relative humidit y at 40°C (104°F)

Unpack and Install

Warning

• The total weight of the package inc luding the unit is 22 kg (48 lbs). Follow regional safety practices when handling, moving, and unpacking shipping boxes and containers.

• Keep hands, loose clothing, and long hair away from the manifold when connecting the gas supply. Slight movement may occur as the unit pressurizes.

Note: Biotage recommend that the boxes and packing materials are retained by the customer in case the unit needs to be transported or stored.

The unit will already have the tubing attached in the back as shown in Figure 1.

1. Open the inner box and remove the smaller boxes. These contain the gas supply installation kit, sealing gasket, etc; check the contents against the packing list on the next page.

2. Gently pull the unit from the box and set the unit on a bench or in a fume hood. See the site requirements above.

3. Using the supplied gas supply installation kit (PPM-GA), plumb the back of the unit to the gas supply (see Figure1). Refer to the Gas Source section above for gas requirements.

4. Position the unit so that the handle and gauges are facing forward and the gas supply is in the rear. Note that the unit weighs 14 kg (31 lbs).

There are a variety of filters available for the removal of moisture, particulates, and hydrocarbons most commonly found within house gas supply systems. The optimum gas supply (80 psi) is achieved by a regulator positioned between the gas source and the unit. The supplied gas supply installation kit (PPM-GA) provides the par ts necessary to make the connection between the gas regulator and the unit.

Minimum pressure 60 psi (4.1 bar)

Maximum pressure 100 psi (6.9 bar)

Optimum pressure 80 psi (5.5 bar)

Figure 1. Connec t the gas line to the right port at t he back of the unit.

 Biotage® PRESSURE+ 48 User Manual | © Biotage 2017

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Packing List

PRESSURE+ 48 is shipped with the following items:

Part Number Description Qty

PPM-A48-GSKT PRESSURE+ 48 Sealing Gasket 48

positions (also contains a hex key)

Installation

PPM-GA PRESSURE+ Gas Supply Adaptor for

PPM-A48WSTRCK

4132 47 User Manual 1

all models (6’ of 1/8” i.d. polyethylene tubing and 1/8” and 1/4” NPT connectors)

PRESSURE + 48 Waste Bin Rack with Insert s (3)

Column Racks and Collection Racks

Note: Column racks and collection racks must be purchased

separately; see the list of racks below.

Part Number Description

PPM-A48-1RCK PRESSUR E+ 48 SPE Column Rack 1 mL

PPM-A48-3RCK PR ESSURE+ 48 SPE Column Rack 3 mL

PPM-A48-6RCK PRESSURE+ 48 SPE Column Rack 6 mL

PPM-A48-1275 PRESSURE+ 48 Collection Rack 12 x 75 mm

PPM-A48-13100 PRESSURE+ 48 Collection Rack 13 x 100 mm

PPM-A48-16100 PRESSURE+ 48 Collection Rack 16 x 100 mm

PPM-A48-1232 PRESSURE+ 48 Sample Vial Rack 12 x 32 mm

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System Description

PRESSURE+ 48 is a positive pressure manifold designed to process SPE columns using a pressurized gas source.

Load the Columns

PRESSURE+ 48 units are compatible with all commonly used 1 mL, 3 mL and 6 mL tabless columns used for sample preparation.

The handgrip on the front of the platform helps you to slide the platform with the racks forward and backward under the manifold. Stack the column rack onto the waste bin rack and populate with the required number of columns. Each column will be pressurized equally even if columns are omitted.

Push the platform and populated rack assembly to the back of the track until it reaches the stop point (under the upper manifold). To lower the manifold, press down on the two rocker switches simultaneously and hold for approximately 3 seconds. This will activate the upper manifold and it will self-adjust to an appropriate height by lowering and compressing. If you let go too soon, the upper manifold will rise up and you can simply repeat by pressing down on the rocker switches and holding them again for approximately 3 seconds.

To release the column rack, press up on both rocker switches, then slide the assembly forward.

Note: The upper manifold is powered by the gas source and the speed is preset and not adjustable.

IMPO RTANT: An important safety feature of the unit is the requirement to hold both rocker switches for approximately 3 seconds simultaneously to lower the manifold. This is to keep hands clear of moving parts during compression.

Figure 2 . Lower the manifold by pressing down on the t wo rocker switches simultaneously for approximately 3 seconds.

 Biotage® PRESSURE+ 48 User Manual | © Biotage 2017

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Flow Control

The PRESSURE+ 48 unit has a two-tier gas delivery system. The flow control switch (B) enables setting and operation of gas flow in addition to turning gas flow on and off:

Adjustable Flow Rate (0 to 15 psi, 0 to 1180 mL/min)

Recommended for sample loading and elution. By turning the flow control switch (B) to the lef t to the Adjustable Flow position, gas is channeled through the Adjustable Flow regulator (C) and then through a rotometer (A) controlled by the needle valve positioned at its base. This path through the rotometer provides ver y precise and slow flow through the columns by limiting gas flow from 0 to 1180 mL/min.

IMPO RTANT: As indicated on the label, never turn the rotometer completely off. Seating the needle valve into its orifice can damage the unit.

System Description

Maximum Flow Rate (0 to 100 psi)

Recommended for column washing, dr ying, etc. By turning the flow control switch (B) to the right to the Maximum Flow position, gas is introduced at the pressure set on the Maximum Flow regulator (D). The pressure range for this regulator is 0 to 100 psi and should be used when processing very viscous or dir ty samples.

Turn Off the Gas

By turning the flow control switch (B) to the OFF position, no gas will flow through the manifold.

IMPO RTANT: The unit should always be in the OFF position during compression and decompression cycles and when not in use. Always shut off the external gas supply when the unit is not in use.

Figure 3. The rotometer (A), flow control switch (B), Adjustable Flow regulator (C), and Maximum Flow regulator (D).

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Operation

Warning

• Keep hands, loose clothing, and long hair away from the manifold when it is moving.

• Only use racks and waste bin inserts supplied by Biotage and ensure to stack the racks properly on the platform to avoid spillage. The racks are physically keyed to the platform by three elevated pins on the platform that recess into the bottom of the rack. When installed properly, the rack should sit level on the platform.

• To prevent personal injury and/or equipment damage, always use impermeable gloves, chemical safety goggles and protective clothing, follow all generally-accepted lab safet y procedures and applicable laws and regulations, and consult your company’s safet y exper t for guidance.

Step-by-Step Instructions

1. Ensure the unit is connected to a gas supply; see Installation section on page 2.

2. Place a waste bin rack and a waste bin insert on the platform; see Figure A.

3. Insert desired number of columns in a column rack. Each column will be pressurized equally even if columns are omitted.

4. Place the column rack on top of the waste bin rack and add sample; see Figure B.

5. Slide the platform back to position the column rack under the manifold; see Figure C.

6. Turn on the gas for the rows in use, i.e. toggle the appropriate gas switch(es) on top of the manifold to the ON position. There are four switches; one switch for each row of columns. See Figure D.

7. Turn the flow control switch to the left to the Adjustable Flow position; see Figure E.

8. Adjust the regulator and rotometer to achieve the desired flow rate; see Figure F.

IMPO RTANT: As indicated on the label, never turn the rotometer completely off. Seating the needle valve into its orifice can damage the unit.

13. Add wash solvent to each column and slide the platform back to position the column rack under the manifold.

14. Lower the manifold by pressing down on the two rocker switches simultaneously for approximately 3 seconds.

15. Turn the flow control switch to the left to the Adjustable Flow position; see Figure E.

16. Turn the flow control switch to the right to the Maximum Flow position and adjust the manifold pressure to the desired setting for the drying period; see Figure I.

17. When the drying period is completed, turn the flow control switch to the OFF position. See Figure H.

18. Raise the manifold by pressing up on the two rocker switches simultaneously.

19. Pull out the platform and replace the waste bin rack with a collection rack; see Figure J.

20. Add elution solvent to each column.

21. Allow the samples to elute by gravity or repeat step 5 through 12 for pressure assisted elution. Tip: To trigger gravity elution, slide the platform back to position the rack under the manifold, ensure the flow control switch is in the OFF position and then lower the manifold. Raise the manifold after a few seconds.

IMPO RTANT: The unit should always be in the OFF position during compression and decompression cycles and when not in use. Always shut off the external gas supply when the unit is not in use.

9. Lower the manifold by pressing down on the two rocker switches simultaneously for approximately 3 seconds; see Figure G.

10. When all liquids have passed through the columns, turn the flow control switch to the OFF position. See Figure H.

11. Raise the pressure manifold by pressing up on the two rocker switches simultaneously.

12. Pull out the platform.

 Biotage® PRESSURE+ 48 User Manual | © Biotage 2017

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Operation

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Maintenance

Replace the Sealing Gasket

You need a sealing gasket with 48 positions and a hex key (PPM-A48-GSKT). In order to maintain compliance, only sealing gaskets supplied by Biotage must be used with the unit.

1. Shut off the external gas supply.

2. Ensure that at least one gas switch on top of the manifold is in the ON position.

3. Turn the unit’s flow control switch to the right to the Maximum Flow position.

4. When there is no remaining pressure in the unit (0 psi), turn the unit’s flow control switch to the OFF position.

5. Remove the two 1/4-20 hex screws that hold the upper manifold to the unit (standoffs) using the hex key.

6. Lift the manifold off the standoffs and place it upside down on the benchtop.

7. Remove the old sealing gasket from the manifold and clean the manifold using ethanol.

8. Remove the backing of the replacement sealing gasket.

9. Align the holes on the new sealing gasket with the manifold’s holes and adhere the seal.

10. Fasten the manifold (with the sealing gasket facing down) to the standoffs using the two hex screws and the hex key.

Note: The adhesive on the sealing gaskets is volatile and cannot be stored for long periods of time at room temperature. For extended storage, place sealing gaskets in refrigerated conditions at 2°C to 5°C

(35.6°F to 41°F).

Clean

Materials of construction are primarily stainless steel and anodized aluminum. Other materials include silicone rubber, polyethylene, and polypropylene. Always clean up spills or overflow immediately.

If you wish to clean the sealing gasket, use a soft cloth, dampened with ethanol or methanol. The sealing gasket is manufactured from silicone rubber.

To clean the manifold, use a soft cloth, dampened with water. Never spray or apply water directly to the manifold.

Note: The controls and gauges are not solvent resistant.

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General Information

Consumables and Accessories

To order consumables and accessories, see contact information on the back of this document or visit our website www.biotage.com.

Part Number Description Qty

PPM-A48-1RCK SPE Column Rack 1 mL 1

PPM-A48-3RCK SPE Column Rack 3 mL 1

PPM-A48-6RCK SPE Column Rack 6 mL 1

PPM-A48-1275 Collection Rack 12 x 75 mm 1

PPM-A48-13100 Collection Rack 13 x 100 mm 1

PPM-A48-16100 Collection Rack 16 x 100 mm 1

PPM-A48-1232 Sample Vial Rack 12 x 32 mm 1

C44651 12 x 75 mm Test Tube, Uncapped 1000

C40707 13 x 100 mm Test Tube, Uncapped 1000

C40708 16 x 100 mm Test Tube, Uncapped 1000

PPM-A48-WST Waste Bin Inserts 3

PPM-A48-WSTRCK Waste Bin Rack with Inserts (3) 1

Biotage Sweden AB

Box 8 SE-751 03 Uppsala SWEDEN

Visiting address: Vimpelgatan 5

Phone: +46 18 56 59 00 Fax: +46 18 59 19 22 E-mail: [email protected] Website: www.biotage.com

Please contact your local Biotage representative. See contact information on the back of this document or visit our website www.biotage.com.

PPM-A48-GSKT Sealing Gasket 48 positions (also

contains a hex key)

PPM-GA PRESSURE+ Gas Supply Adaptor for

all models (6' of 1/8" i.d. polyethylene tubing and 1/8" and 1/4" NPT connectors)

For a complete list, please visit our website www.biotage.com.

Manufacturer

Made in USA

for Biotage Sweden AB

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Your Complete Partner for Effective Chemistry

Biotage is a worldwide supplier of instruments and accessories designed to facilitate the work of laboratory and process chemists. With our deep knowledge of the industry, academic contacts and in-house R&D teams, we can deliver the best solutions to your challenges. We take great pride in our flexibility and ability to meet our customer’s individual needs. With strong foundations in both analytical, organic and process chemistry, we can offer the widest range of solutions available on the market.

Title Truncated if Neccessar y... | Page 

EUROPE

Main Of fice: +46 18 565900

Toll Free: +800 18 565710

Fax: +46 18 591922

Order Tel: +46 18 565710

Order Fa x: +46 18 565705

[email protected]

Suppor t Tel: +46 18 56 59 11

Suppor t Fax: + 46 18 56 57 11

[email protected]

Part Numb er: 413247-C

© 2017 Biotage. A ll rights r eserve d. No materi al may be repr oduced or p ublished w ithout the wr itten p ermissi on of Biotag e. Informa tion in this d ocument is s ubject to c hange with out notice a nd does not r eprese nt any commit ment from B iotage. E&O E. A list of all t rademar ks owned by Bi otage AB is ava ilable at ww w.biotage.c om/legal. O ther pro duct and co mpany names me ntioned herein m ay be tradem arks or re gistere d tradema rks and/or se rvice ma rks of thei r respec tive owner s, and are us ed only for explana tion and to th e owners’ b enefit, wi thout inte nt to infrin ge.

NORTH & LATIN AMERICA

Main Of fice: +1 704 654 4900

Toll Free: +1 800 446 4752

Fax: +1 704 654 4917

Order Tel: +1 704 654 4900

Order Fa x: +1 434 296 8217

[email protected]

Suppor t Tel: +1 800 446 4752

Outside US: +1 704 654 4900

[email protected]

JAPAN

Tel: +81 3 5627 3123