Biosan FTA-1, FTA-2i User Instructions

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Contents
1. About this edition of user instructions ....................................................................... 3
2. Safety precautions ................................................................................................... 4
3. General information .................................................................................................. 5
4. Getting started .......................................................................................................... 6
5. Operation ................................................................................................................. 9
6. Specifications ......................................................................................................... 10
7. Care and maintenance ........................................................................................... 12
8. Warranty ................................................................................................................. 14
9. EU Declaration of conformity .................................................................................. 15
The current edition of the user manual applies to the following models and versions
of aspirators with trap flasks:
FTA-1 version V.4AW FTA-2i version V.1A01
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2. Safety precautions
Caution! Make sure you have fully read and understood the present Manual
before using the equipment. Please pay special attention to sections marked by this symbol.
GENERAL SAFETY
The protection provided can be ineffective if the operation of the appliance does not
comply with the manufacturer's requirements.
Save the unit from shocks and falling. Store and transport the unit at ambient temperatures between -20°C and +60°C and
maximum relative humidity of 80%.
After transportation or storage and before connecting it to the electric circuit, keep the
unit under room temperature for 2-3 hrs.
Use only original parts and accessories, provided by manufacturer for this product. Before using any cleaning or decontamination methods except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not damage the equipment.
Do not make modifications in design of the unit.
ELECTRICAL SAFETY
Connect only to the mains with voltage corresponding to that on the serial number
label.
Use only the external power supply provided with this product. Ensure that the power plug is easily accessible during use. Disconnect the unit from the mains before moving. If liquid penetrates into the unit, disconnect it from the mains and have it checked by
a repair and maintenance technician.
Do not operate the unit in premises where condensation can form. Operating condi-
tions of the unit are defined in the Specifications section.
DURING OPERATION
Do not operate the unit in environments with aggressive or explosive chemical mix-
tures. Please contact manufacturer for possible operation of the unit in specific at­mospheres.
Do not operate the unit if it is faulty or has been installed incorrectly. Do not use outside laboratory rooms. Do not use the unit for aggressive organic compounds, chlorinated solvents and
strong conjugated acids.
BIOLOGICAL SAFETY
It is the user’s responsibility to carry out appropriate decontamination if hazardous
material is spilt on or penetrates into the equipment.
Empty the trapping flask when the liquid level reaches maximum. Take necessary
precautions utilizing waste liquid in accordance with general laboratory standards.
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3. General information
FTA-1 & FTA-2i aspirators with trap flask have been designed for liquid removal from
micro-test tubes (e.g. for supernatant removal during DNA/RNA extraction), ELISA plates (e.g. performing several repeated well washing cycles), cell culture flasks (cell media ex­change and washing during cell growth) and other vessels during various laboratory tech­niques.
Model FTA-1 has a 1 L glass trap flask and is designed to be a low-cost solution.
Aspiration is possible with a single standard 200 μL tip and the 8-tipped MA-8 adapter.
Model FTA-2i comes with a 2 L polypropylene trap flask and a liquid overflow sensor that stops collected material from damaging the filter. Aspiration is possible with single 200 and 1000 μL tip using the MA-U adapter or with the handheld vacuum controller HAS-1 with different attachments such as 8-channel adapter with stainless steel tips, 8-channel adapter for 200 μl tips, single channel adapter for 200 μl tips and others.
Microbiologic suction filter eliminates risks of contamination caused by bacteria, vi­ruses, and infected particles from samples. Such contamination can be distributed by suction pump or central vacuum units. Microbiological suction filter is hydrophobic and has very high contamination removal bacterial efficiency – up to 99.99% of particles bigger than 0.027 micrometres (e.g. Hepatitis A, B and C, HIV, etc.).
In order to prolong performance and efficiency of device it is not allowed to use ag­gressive organic compounds (e.g. phenol) and strong conjugated acids (e.g. guanidine) for aspiration. Please, contact manufacturer in case of any questions regarding operation of device.
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4. Getting started
4.1. Unpacking. Remove packing materials carefully and retain them for future shipment
or storage of the unit. Examine the unit carefully for any damage incurred during
transit. The warranty does not cover in-transit damage. Warranty covers only the units
transported in the original package.
4.2. Complete set. Package contents:
4.2.1. FTA-1:
- FTA-1, aspirator with trap flask ........................................................................ 1 pce.
- Hydrophobic filter ............................................................................................. 1 pce.
- Aspiration tips, 200 µL .................................................................................... 50 pcs.
- Holder tubes (1.5 ml) ..................................................................................... 10 pcs.
- Spare hydrophobic filter ................................................................................... 1 pce.
- External power supply ..................................................................................... 1 pce.
- Operating manual, declaration of conformity .................................................. 1 copy
- MA-8, 8-channel aspiration tip with holder ................................................ on request
4.2.2. FTA-2i:
- FTA-2i, aspirator with flask trap ........................................................................ 1 pce.
- MA-U, universal adapter .................................................................................. 1 pce.
- Hydrophobic filter ............................................................................................. 1 pce.
- Aspiration tips, 200 µL .................................................................................... 50 pcs.
- Aspiration tips, 1000 µL .................................................................................. 10 pcs.
- Holder tubes (2 ml) ................................ .......................................................... 5 pcs.
- Spare hydrophobic filter ................................................................................... 1 pce.
- External power supply ..................................................................................... 1 pce.
- Operating manual, declaration of conformity .................................................. 1 copy
- MA-8, 8-channel aspiration tip with holder ................................................ on request
- HAS-1, hand operator set with SH-6 stand ............................................... on request
Figure 1. Assembled FTA-1 Figure 2. Installation of MA-8 aspiration tip
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Figure 3. FTA-2i front view Figure 4. FTA-2i lid with sensor and tubing
Figure 5. MA-U, universal adapter
Figure 6. FTA-2i filter setup Figure 7. FTA-2i rear panel
4.3. FTA-1 setup.
- Place the unit on horizontal even working surface.
- Install the trapping flask (fig. 1/5) on the unit.
- Assemble the lid, aspiration tubing and filter set (fig. 1/2). Attach aspiration tip
(fig. 1/6) to the tubing. Caution! Position the filter with the IN marking pointing towards the unit (fig. 1/2).
- Screw on the lid with the filter set and aspiration tubing to trap flask. Ensure that the
filter set is tightly inserted.
- Connect the filter tubing to the pump (fig. 1/1) located at the top of the base behind
the trap flask, by turning the fitting clockwise.
- Place the microtubes for aspiration tip into the holder (fig. 1/4).
- Connect the external power supply unit into the socket at the rear side of the unit and
position the unit for an easy access to the external power supply and the power switch.
4.3.1. Optional MA-8 aspiration tip installation. Remove microtubes and aspiration tip
from the holder (fig. 1/4). Place MA-8 storing holder (fig. 2/1) into the holder. Remove
aspiration tip (fig. 1/6) from the aspiration tubing and connect the MA-8 aspiration tip
(fig. 2/2). Store the aspiration tip in the new holder.
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4.4. FTA-2i setup.
- Place the unit on horizontal even working surface.
- Install the trapping flask (fig. 3/2) on the unit.
- Screw on the lid with the sensor needles and valves for tubing (fig. 3/1) to trapping flask.
- Assemble the filter set (figure 6) and connect it to the port on the rear panel of the
unit (fig. 7/1). Caution! Position the filter with the IN marking pointing away from the unit (fig. 6/1).
- Attach the fitting (fig. 4/1) of the tubing to the filter (fig. 4/2) to the valve with white
ring on the lid.
- Ensure that the filter set is tightly fixed in the slot (fig. 7/1).
- Assemble the MA-U aspiration tip set (figure 5).
- Attach the fitting (fig. 4/3) of the tubing for liquids (fig. 4/4 and 5/3) to the valve with
the blue ring on the lid.
- Connect the sensor cable (fig. 4/5) to the lid of the unit and to the port on the rear
panel of the unit (fig. 5/3).
- Place the microtubes for aspiration tip into the holder (fig. 3/3).
- Connect the external power supply unit into the socket at the rear side of the unit (fig.
7/4) and position the unit for an easy access to the external power supply and the
power switch.
4.4.1. Optional MA-8 aspiration tip installation. Remove microtubes and aspiration tip
from the holder (fig. 3/3). Remove the aspiration tip (fig. 5/1) from the MA-U adapter
and connect the MA-8 aspiration tip (fig. 8/1).
4.4.2. Optional HAS-1 hand operator installation. Unscrew the MA-U adapter from the
aspiration tube (fig. 5/3) and connect the HAS-1 hand operator (fig. 8/) by screwing
it to the fitting.
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5. Operation
5.1. Connect the external power supply to the mains.
5.2. Turn the Power switch (fig. 1/3 or 7/2) to the position I (on). The pump starts working.
5.3. (For model FTA-2i) Regulate the aspiration intensity with the regulator knob on the
front panel of the unit (fig. 3/4). Clockwise turning direction increases power, counter
clockwise – decreases. Current intensity is indicated by a light ring around the regu-
lator knob.
5.4. Touch the surface of the liquid with the aspiration tip (fig. 1/6, 5/1 or MA-8) to remove
the liquid. Caution! During operation, do not allow the collected liquid to exceed the max-
imum level. Empty the trapping flask when the liquid level reaches maximum allowed level, according to 5.7.
Note. In model FTA-2i, when collected liquid exceeds the maximum level,
the sensor turns off the pump and produces sound and light warning signals.
5.5. (For model FTA-2i) When using the optional HAS-1 hand regulator, touch the liquid
with the aspiration tip, then press and hold the blue button on the regulator to collect.
5.6. After finishing the operation, switch off the Power switch and disconnect the external
power supply from the mains.
5.7. Emptying the trapping flask. Turn off the unit. Remove the tubings and cables from
the lid. Remove the closed flask from its place. Unscrew the lid and empty the trap-
ping flask. Screw the lid back on and return the trapping flask on the unit. Replace
the tubings and the cables. Caution! Take necessary precautions in utilizing waste liquid in accordance
with general laboratory standards. For information regarding auto­claving procedures, see 7.4.
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6. Specifications
The unit is designed for operation in cold rooms, incubators (except CO2 incubators)
and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non-condens-
ing atmosphere and maximum relative humidity 80% for temperatures up to 31°C decreasing
linearly to 50% relative humidity at 40°C.
Biosan is committed to a continuous programme of improvement and reserves the right to alter design and specifications of the equipment without additional notice.
FTA-1
FTA-2i
Aspiration speed
up to 1.7 mL/s
up to 27.8 mL/s
Vacuum regulation
None
Yes
Working vacuum pressure
-500 mbar
-200 … -800 mbar
Overflow protection sensor
None
Yes, invasive
Overflow protection actions
None
Pump stop, sound and light signals
Trapping flask
1 L, glass
2 L, polypropylene (PP)
Air filtration
Hydrophobic microbiologic air filter 2200/021
Filter pore diameter
0.027 μm
Input current
DC 12 V, 300 mA
DC 12 V, 1 A
Power consumption
3.6 W
10.8 W
External power supply
input AC 100-240 V, 50/60 Hz; output DC 12 V
Dimensions
160x210x340 mm
185x290x390 mm
Weight2
1.7 kg
1.85 kg 
Figure 8. Optional accessories and replacement parts
1
Eliminates risk of contamination from the trapping flask with bacteria, viruses and infected particles
2
Accurate within ±10 %
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Figure 8. Optional accessories and replacement parts (continued)
Table 1. Optional accessories
Description
Catalogue number
МА-8, 8-channel aspiration tip with holder (fig. 8/1)
BS-040108-BK
HAS-1, hand operator set (only for FTA-2i). Set includes:
Handheld vacuum controller with adapter (fig. 6/),
8-channel adapter (with ejector) for 200 µL tips (fig. 6/),
1-channel adapter with 125 mm stainless steel pin (fig. 6/),
1-channel adapter with 40 mm stainless steel pin (fig. 6/), 8-channel adapter with 35 mm stainless steel pin (fig. 6/),
1-channel adapter (with ejector) for 200 µL tips (fig. 6/3),
Stand SH-6 for hand operator parts (fig. 6/)
BS-040118-PK
Table 2. Replacement parts for FTA-1
Description
Catalogue number
Hydrophobic filter
BS-040108-S25
Aspiration tip, 200 µL, 1000 pcs. (fig. 8/)
BS-000001-S04
Aspiration tip, 8 channels, for MA-8 (fig. 8/1)
BS-040108-S24
Trapping flask, 1 L, glass, with lid, inserts and sealing ring
BS-040108-S11
Tubing and fitting set
BS-040108-S22
Table 3. Replacement parts for FTA-2i
Description
Catalogue number
MA-U, universal adapter for 200/1000 µL single use tips (fig. 8/)
BS-040118-AK
Hydrophobic filter
BS-040108-S25
Aspiration tip, 200 µL, 1000 pcs. (fig. 8/)
BS-000001-S04
Aspiration tip, 1000 µL, 1000 pcs. (fig. 8/)
BS-000001-S05
Aspiration tip, 8 channels, for MA-8 (fig. 8/1)
BS-040108-S24
Trapping flask, 2 L, polypropylene, with lid, inserts and sealing ring
BS-040120-S11
Tubing and fitting set
BS-040120-S14
Sensor cable
BS-040120-S12
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7. Care and maintenance
7.1. If the unit requires maintenance, disconnect the unit from the mains and contact Bio-
san or your local Biosan representative.
7.2. All maintenance and repair operations must be performed only by qualified and spe-
cially trained personnel.
7.3. Cleaning and disinfection.
7.3.1. Glass and plastic outer parts. Standard ethanol (75%) or other cleaning agents rec-
ommended for cleaning of laboratory equipment can be used for cleaning and decon-
tamination.
7.3.2. Silicone and internal parts. Use only distilled water, no less than 0.5 L.
7.4. Autoclaving procedures.
Lid, fittings,
tubing
Flask
Hydrophobic filter,
cables, base pump
FTA-1
121°C and 1
atm., 15 min.
121°C and 1 atm., 55 min.
Do not autoclave
FTA-2i
121°C and 1 atm., 15 min.
Caution! Do not put a closed flask in the autoclave, as it might irreversibly de-
form. Completely disengage the thread on the cap.
Note. Hydrophobic filter and sensor cable are sensitive to moisture and are
not autoclavable.
Tubing is made of silicone and deteriorate after frequent autoclaving
and should be replaced.
7.5. Tubing replacement. Change the silicone tubing every 6 months.
7.6. Filter replacement. Replace the filter as soon as it accumulates visible traces of dirt,
or when the liquid is sucked into it. Maximum working time for the filter is 30 days.
When working with infected materials, replace the filter immediately after finishing the
procedure to prevent the spread of infection.
7.6.1. Changing procedure. Remove the filter (fig. 1/2 or 6/1) by detaching it from adapters.
Insert a new filter and reconnect the adapters. Caution! Observe the orientation of the filter:
For model FTA-1, the IN marking should be facing towards the unit (figure 1). For model FTA-2i, the IN marking should be facing away from the unit (figure 6).
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7.7. Troubleshooting.
Problem
Possible reason
Troubleshooting
Unit not working
No power
Check external power supply and
connector
(FTA-2i) Trapping flask full
Sensor shut down the motor. Empty
the trapping flask according to 5.7.
(FTA-2i) Sensor not connected
properly
Check sensor cable and attach-
ments
Aspiration slow or
absent
(FTA-2i) Aspiration intensity set
to small
Turn the regulator knob clockwise to
increase aspiration.
Breach in the system
Check all the tubing and fittings.
Tightly close the lid of the unit
Dirt in the tubing
Clean the tubing, see 7.3.
Liquid in the filter
Replace the filter
(FTA-2i) Sensor
activated, but the
flask is not full
Foam in the trapping flask acti-
vated the sensor
Do not use the unit with a foaming
liquid or use antifoaming agent.
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8. Warranty
8.1. The Manufacturer guarantees the compliance of the unit with the requirements of
Specifications, provided the Customer follows the operation, storage and transporta-
tion instructions.
8.2. The warranted service life of the unit from the date of its delivery to the Customer is
24 months. For extended warranty, see 8.5.
8.3. Warranty covers only the units transported in the original package.
8.4. If any manufacturing defects are discovered by the Customer, an unsatisfactory
equipment report shall be compiled, certified and sent to the local distributor address.
To obtain the claim form, visit section Technical support on our website at link be-
low.
8.5. Extended warranty.
For FTA-2i, the Premium class model, one year of extended warranty is available
free of charge after registration, during 6 months from the date of sale. Online regis-
tration form can be found in section Warranty registration on our website at the link
below.
For FTA-1, the Basic Plus class model, extended warranty is a paid service. Contact
your local Biosan representative or our service department through the Technical
support section on our website at the link below.
8.6. Description of the classes of our products is available in the Product class descrip-
tion section on our website at the link below.
Technical support
Registration
Product class description
biosan.lv/en/support
biosan.lv/register-en
biosan.lv/classes-en
8.7. The following information will be required in the event that warranty or post-warranty
service comes necessary. Complete the table below and retain for your records.
Model
FTA-1 & FTA-2i, aspirators with trap flask
Serial number
Date of sale
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9. EU Declaration of conformity
Unit type Aspirators with trap flask Models FTA-1, FTA-2i Serial number 14 digits styled XXXXXXYYMMZZZZ, where XXXXXX is
model code, YY and MM – year and month of production, ZZZZ – unit number.
Manufacturer SIA BIOSAN
Latvia, LV-1067, Riga, Ratsupites str. 7/2
Applicable Directives EMC Directive 2014/30/EU
LVD Directive 2014/35/EU RoHS2 2011/65/EU WEEE 2012/19/EU
Applicable Standards LVS EN 61326-1: 2013
Electrical equipment for measurement, control and laboratory use. EMC requirements. General requirements.
LVS EN 61010-1: 2011
Safety requirements for electrical equipment for measurement, control, and laboratory use. General requirements.
We declare that this product conforms to the requirements of the above Directives
____________________ ____________________
Signature Signature
Svetlana Bankovska Aleksandr Shevchik Managing director Engineer of R&D
____________________ ____________________
Date Date
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Biosan SIA
Ratsupites 7, build.2, Riga, LV-1067, Latvia Phone: +371 67426137 Fax: +371 67428101
http://www.biosan.lv
Edition 1.-4.01 – March 2018
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