Bioptechs PERFHEATEDLID User Manual

Delta T Heated Culture Dish Cover with Perfusion Ports

Instructions

Description:

The Bioptechs Delta T® Heated Lid w/ Perfusion is a device, which will provide a condensate free optical surface
on the top of a Delta T® Dish through which specimens can be trans-illuminated on an inverted microscope. It
also provides a port for the introduction of gas above the specimen as well as perfusion ports for the exchange of
media.

Instructions:

When preparing to use the heated lid, it should be pre-warmed by either placing it in an incubator or connecting
it to the heated lid output socket in the back of the controller for about 15 minutes. Connection is made to the
Heated Lid through a three pin 0.1 pin header. Both of the outer pins are earth ground, the center pin carries
current. Therefore, orientation of the connector does not matter. The controller end of the connector wire is a
standard 5.5mm DC coaxial plug.

After the Heated Lids is warmed, it can be mounted to the Delta T® dish by applying a combination of downward
and slight rotational force to the lid in such a manner to compress the o-ring seal to seat the lid on the dish.
Removal also requires a slight rotating motion. It is powered by a 2.5-volt adjustable power supply located on
the back of the Delta T®4 Controller. The voltage should be adjusted to a point where the temperature of the
glass surface of the lid prevents condensation in the dish. The controller is factory set to 2.5 volts. However, It
may be necessary to adjust the voltage depending on ambient conditions. An adjustment screw for this setting is
provided adjacent to the Heated Lid Socket on the back of the Delta T4 controller. Alternate power supplies can
be used including a DC battery or an AC adapter.

Note: A heat lter set is recommended in the transmitted light path. (Reection lter before absorption lter).

Cleaning:

The heating lid can be cleaned with mild laboratory soap and water, rinsed and dried. If sterilization is a concern
it can be placed in an autoclave bag and autoclaved on a short cycle of about 15 minutes. Exercise extreme care
in handling.

Note: After autoclaving it must be thoroughly dry on the outside surface before use.

Important!

Do not apply electrical power to the lid if liquids are present on the coated surface of the lid (same surface as
contacts). Make sure the lid is dry before use.

Perfusion

Specimens can be perfused by attaching perfusion tubing to the ports provided. It is recommended to use the
Bioptechs Micro-Perfusion Pump with the dual perfusion tubing for this purpose. A diagram of this conguration is
available at: http://www.bioptechs.com/Products/Delta_T/D_Perf/d_perf.html.

3560 Beck Road • Butler, PA 16002 • Voice: 724-282-7145 • Fax: [phone] • E-mail: [e-mail] • Web: www.bioptechs.com