3560 Beck Road • Butler, PA 16002 • Voice: 724-282-7145 • Fax: 724-282-0745 • E-mail: info@bioptechs.com • Web: www.bioptechs.com
Delta T Hinged Perfusion Adapter Set Instructions
The Bioptechs Hinged Perfusion Supports provide Delta T users with a convenient and inexpensive method of supporting
perfusion needles in the culture dish. A typical application is to maintain low volume perfusion over cells during longterm experiments. Perfusion supports are sold in pairs, one hinge and needle is for irrigation, the other is for aspiration.
The balance between irrigation and aspiration can be maintained continuously with the use of the Delta T Micro-Perfusion
Pump*. Additional supports can be added to hold gas jets, pH probes, cooling apparatus, or other items which do not
require critical positioning.
Benets:
• Needles compatible with 1/16” tubing
• User adjustable friction for reliable positioning
• Repeatable positioning, ips in and out to replace dishes
• Adjustable pick-up tube to control level of media in dish*
• Perfusion assembly translates with the stage adapter and dish
• Eliminates the need for expensive micromanipulators for low precision positioning
*Bioptechs recommends the use of the Delta T Micro-Perfusion Pump for closely regulated perfusion.
Instructions:
Press t the 1/8” ends of the hinge adapter into the stage adapter. Adjust the tension of the hinge with the 5/64” Allen
driver provided. The hinge can be rotated or adjusted up and down as needed. Press tubing onto the 14 gauge needles and
attach to uid irrigation and aspiration as noted below.
Continual Delta TC3 Perfusion with the Delta T Micro-Perfusion Pump:
The Delta T Micro-Perfusion Pump is shipped with dual channel tubing. When the tubing pair is stretched around
the rollers on the pump, the tube with the black band will have a slightly smaller ID yielding a slower uid transport
capability. Attach the supply line from your media to the barb on the input side of the black band pump tubing and
another tube from the output side of this tube to the selected irrigation port of the dish. Attach tubing from the aspiration
port of the dish to the input side of the other pump tube and a line from the output side of this pump tube to a waste
container. Adjust the pump speed as needed for suitable perfusion rate. Adjust the depth of the aspiration needle to obtain
the desired level of media in the dish.
Note: Please refer to the back of this page for an instructional diagram.
3560 Beck Road • Butler, PA 16002 • Voice: 724-282-7145 • Fax: 724-282-0745 • E-mail: info@bioptechs.com • Web: www.bioptechs.com
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