Bioptechs FCS3PERFUSION User Manual

Perfusion Conguration for Induced Change Experiments

®

6 Luer adapter (male, adapts 1/16" tubing to female Luer lock)

7 Alternate media glass syringe (growth factors, inhibitors, etc.)

8 Three-way valve (switches source of perfusion; pump or manual)

9 FCS3 Chamber

10 Tubing to waste container

11 Tee adapter (place at same height as chamber, open top of tee to prevent siphoning)

Note: Syringes are glass to prevent contamination of media from silicone lubricant typical in plastic syringes.

Typical FCS3

in delicate optical cavities)

loading chamber)

1 Perfusion tubing, 1/16" ID supply line (keep as short as possible)

2 FCS3 Micro-Perfusion Pump (recommended for prolonged, controlled perfusion

3 Tee adapter (adds low volume syringe to facilitate manual perfusion when

4 Luer adapter (female, adapts Luer taper syringe to 1/16" tubing)

5 Low volume glass syringe

3560 Beck Road • Butler, PA 16002 • Voice: 724-282-7145 • Fax: [phone] • E-mail: [e-mail] • Web: www.bioptechs.com