Bioptechs DT4COOLINGRING User Manual

3560 Beck Road • Butler, PA 16002 • Voice: 724-282-7145 • Fax: [phone] • E-mail: [e-mail] • Web: www.bioptechs.com

Delta T Cooling Ring Instructions

Description:

The Bioptechs cooling ring is an immersion device, which provides a thermally conductive physical barrier between
chilled uid passing through the ring and the uid surrounding the specimen thus absorbing heat from the specimen. This
cooling ring is made of autoclavable 304 stainless steel and provides the microscopist with a convenient and inexpensive
method of reducing the temperature of specimens in Delta T Culture Dishes. The cooling ring is supported on the stage
adapter and translates along with the dish. It is easily ipped up and out of the way to enable easy exchange of dishes in
the stage adapter.

Instructions:

1. Mount an empty Delta T Dish into the stage adapter.

2. Press t the 1/8” end of the hinged cooling ring adapter into the holes provided adjacent to the rectangular
opening on the stage adapter.

3. Using the 5/64” Allen driver provided, adjust the immersion ring to t into the dish and ip up and down as
needed to replace dishes.

4. Adjust the tension of the hinge with the 5/64” wrench.

5. Press 1/16” Tygon tubing onto the 14 gauge tube and attach to a chilled uid source and drain.
Note: A simple Dewar of ice water above the stage, Tygon tubing, and a pinch clamp will reduce the
temperature in the dish to 4 - 10°C. It is simplistic, inexpensive and it works!

6. When you need temperatures above ambient simply stop the chilled water ow and turn on the Delta T
Controller.

3560 Beck Road • Butler, PA 16002 • Voice: 724-282-7145 • Fax: [phone] • E-mail: [e-mail] • Web: www.bioptechs.com