Bioptechs DELTAT4CONTROLLER User Manual

3560 Beck Road Butler, PA 16002 Voice: 724-282-7145 Fax: 724-282-0745 E-mail: info@bioptechs.com Web: www.bioptechs.com
Delta T4 Culture Dish System Instructions
Caution: Read Carefully Before Use!
The System is comprised of three main components: a temperature controller, a stage adapter, and the Delta T Dish. It utilizes a new and improved technology that is much faster and more accurate than traditional peripheral stage warmers. The controller has a fast learning curve to anticipate changes in the dish temperature due to perfusion or surface evaporation while regulating temperature and compensating for entropy. The fast response capability of this system enables control features previously not possible with conventional peripheral systems. Therefore, it is highly recommended that you read and fully understand these instructions before use.
Before You Begin:
Check to make sure there is no visible damage in shipment and save all packing materials.
The Controller box should contain; controller, power cord, 2.5mm screwdriver, 6’ BNC cable, 1/8” jack, and 3 pin mini DIN
connector.
Check the packing list and call your supplier if there are any discrepancies.
Figure 1
Control Interface Descriptions
Numeric Display should be illuminated when the controller is turned on. If it does not light, check the main power source then the fuse block
located in the power input module where the AC power cord plugs in.
Display Selector is the black button adjacent to the display. It selects the source of the information displayed. When the controller is turned on, the default condition is to display the setpoint value indicated by the light adjacent to the word Setpoint. The button selects one of a sequence of display options. This button can be pressed at any time and has no effect on the temperature regulation functions of the controller.
Display Lights indicate the source of the value displayed on the numeric display.
Setpoint is adjusted by a potentiometer accessible with the 2.5mm screwdriver provided. To adjust the setpoint make sure the numeric display is
set to Setpoint, then adjust the setpoint to the desired value. See adjusting setpoint value. If you need to change the setpoint value during your experiment on a routine basis there is a remote setpoint jack on the back of the controller. See Remote Setpoint.
Dish is the location where the calibration of the dish thermistor reading circuit can be adjusted.
Reference is the location where the calibration of the thermistor reading circuit can be adjusted.
Adjust Volts is the location where the non-dynamic voltage adjustment is made. This is the energy value sent to the dish when the controller is in Imaging Mode. Adjust Volts must be selected for the display and the Mode switch must be pressed for this value to appear. The display will show
a number that is 10 times the actual voltage value being sent to the dish. Adjustments can be made to this value by turning the small screw located in the hole corresponding to Imaging Volts.
Mode button changes the mode of operation of the controller from dynamic feedback control to a xed voltage set by the Adjust Volts setting. When the controller is in Dynamic mode, the amber LED will be lit next to the mode switch. When the controller is in Imaging mode, the green LED will light. If the controller is in Imaging mode as a result of pressing the mode switch, only the green LED will light. If the controller is in Dynamic mode and a TTL voltage is applied to the BNC jack on the back of the controller, both the amber and the green LEDs will light. Caution! Make sure that the controller is not left in the Imaging mode for longer than 30 seconds. This will not damage the controller but if the temperature in the dish deviates more than the error margin during this time, the alarm will sound and the controller will ignore all the safety interrupts. The controller will continue to supply the constant voltage to the dish until the controller is returned to the Dynamic mode.
3560 Beck Road Butler, PA 16002 Voice: 724-282-7145 Fax: 724-282-0745 E-mail: info@bioptechs.com Web: www.bioptechs.com
Reset (Red Button) resets the controller to the cold start condition and enters the energy value used in Adjust Volts as a starting regulation value.
It is to be used when any of the following conditions occur:
1. Immediately before placing a new dish into the stage adapter if you want to change dishes without turning off the controller.
2. Immediately after placing a new dish into the stage adapter. This is insurance that the controllers learning curve did not wonder during the few seconds it takes to insert a dish. Dish exchanges should not take more than 10 seconds.
3. When the alarm goes off and the red LED lights next to the Reset button. Note: If the alarm went off there must have been a reason. Check the dish before resetting the controller.
Calibrate substitutes precision resistors into the circuit that reads the thermistors so that calibration adjustments can be made to a known value.
Stage is a six pin mini DIN connector where the stage adapter is plugged in.
Back Panel of the Delta T4 Controller
Figure 2
AC Power line input, power switch, voltage selector, and fuses are all incorporated in the AC Power Input Module. The controller can operate between 90 - 260V 50-60Hz, or a 24V battery can be used as an alternate power supply. The fuse carrier contains two 0.5A slow blow fuses. The fuses can be accessed by prying the fuse block out of its socket with a small screwdriver wedged under the small slot in the opening of the AC line receptacle. When replacing the fuse block, make sure to align the arrow on the outside of the fuse block with the closest voltage value to your supply line voltage:
For 90-130 supply line voltage place the fuse block with the arrow pointing to 110-120V.
For 180-260 supply line voltage place the fuse block with the arrow pointing to 220-240V.
Heated Lid Adjustment is the adjustment for the voltage sent to the Heated Lid. Its range is from 0 to 5 V DC. The nominal voltage is 2.5 V. This voltage should be measured at the Heated Lid with an external meter. It should be adjusted to a value just above the condensation point.
Heated Lid is the location to plug in the Bioptechs Heated Lid.
Temp Out is a voltage output direct from two instrument ampliers reading the thermistors from channel A and B.
The output from this socket is temperature /10. 37.0°C = 3.70 Volt DC. Left pin is channel A, center pin is channel B, and right pin is ground.
Remote Setpoint is an analog input that allows the user to set the controllers setpoint from an external DC source. The sleeve is earth ground. A DC voltage can be applied to the tip of this jack equal to temperature /10. i.e.: 28.5°C = 2.85 Volt. When the jack is inserted into the controller it will automatically switch to the jacks value. When removed it will default to the setting on the front of the controller. Use for programming or cycling operating temperatures.
Fixing Adjustment allows the user to adjust the voltage sent to the dish when the Fixing Switch is depressed. Range from operating temp to 97°C.
Fixing Switch is provided so that cells can be xed on the stage of the microscope. It can also be used for heat shock experiments. The switch is
recessed so that it is not actuated by mistake. Caution! This switch should be used when you intentionally want to overheat your cells. It provides a nearly instantaneous heat shock to the dish.
TTL is a BNC connection used to change the mode of operation of the controller from a remote source such as a computer or slave from a shutter trigger. The default condition of the controller is dynamic control. To change to Imaging mode, apply a TTL logic high and hold to this BNC. The controller will remain in Imaging mode as long as the TTL condition remains high as indicated by the LED on the front panel.
3560 Beck Road Butler, PA 16002 Voice: 724-282-7145 Fax: 724-282-0745 E-mail: info@bioptechs.com Web: www.bioptechs.com
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