Bioptechs CULTURECYLINDER User Manual

Glass Culture Cylinder Instructions

Description:

Culturing Cylinders are used to barricade cells or suspended specimens in a Delta T Dish or to restrict and concentrate
the growth and location of cells plated on a coverslip. They are 5mm high and available in a variety of inside diameters
including, 4mm, 6mm, 8mm, 10mm, 12mm, and 14mm. The outer diameter is always 2mm greater than the inside
diameter due to the 1mm wall thickness. The cylinders are made of Pyrex glass which are heavier than plastic cloning
rings to eliminate oating. They are optically polished on the bottom surface to mate with and form a tight seal with other
glass surfaces such as coverslips and Delta T Dishes. Culture Cylinders can be autoclaved for reuse.

Delta T Application:

When using Culturing Cylinders with Delta T System, select the appropriate size cylinder for the protocol and place the
cylinder into the center of the dish. Pipette the specimen into the cylinder then pipette media around the cylinder to a
depth equal to the depth in the cylinder thereby equalizing hydrostatic forces.
Make sure to maintain a minimum 2ml of media in the dish to maintain its thermal properties. Several cylinders can be
used at the same time in a single dish for multiple specimens.

FCS2 Application:

Place the cylinder onto the center of the 40mm FCS2 coverslip to restrict plating of the cells to the central region of the
coverslip. Apply media around the cylinder to equalize hydrostatic forces. After cells have grown to conuence remove
cylinder and transfer coverslip to the FCS2 for micro-observation.

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