Proline
®
Käyttöohje
Bruksanvisning
Instruction Manual
Käyttöohje ....................................................1
Bruksanvisning .......................................... 17
Instruction Manual .................................... 33
Specifications ............................................ 48
Proline
®
Grip cover:
metal blue 721060
grey 721752
yellow 721825
red 721706
blue 721221
green 721792
Handle,
complete, in gray colour
0.1-2.5 µl 721061
0.5-10 µl 721062
2-20 µl 721063
5-50 µl 721064
10-100 µl 721065
20-200 µl 721082
50-200 µl 721066
100-1000 µl 721067
200-1000 µl 721068
1-5 ml 721069
Piston assembly:
0.1-2.5 µl 721145
0.5-10 µl 721141
2-20 µl 721150
5-50 µl 721150
10-100 µl 721160
20-200 µl 721160
50-200 µl 721160
100-1000 µl 721175
200-1000 µl 721175
1-5 mI 721173
Piston:
0.1-2.5 µl 721146
0.5-10 µl 721142
2-20 µl 721151
5-50 µl 721151
10-100 µl 721161
20-200 µl 721161
50-200 µl 721161
100-1000 µl 721176
200-1000 µl 721176
1-5 ml 721177
Piston spring:
0.1-2.5 µl 721143
0.5-10 µl 721143
2-20 µl 721152
5-50 µl, 721152
10-100 µl 721162
20-200 µl 721162
50-200 µl 721162
100-1000 µl 721465
200-1000 µl 721465
1-5 ml N/A
Spring support:
0.1-2.5 µl 721144
0.5-10 µl 721144
2-20 µl N/A
5-50 µl N/A
10-100 µl 721144
20-200 µl 721144
50-200 µl 721144
100-1000 µl 721464
200-1000 µl 721464
1-5 mI N/A
O-ring, set of 10 pieces
0.1-2.5 µl N/A
0.5-10 µl 721090
2-20 µl 721100
5-50 µl 721100
10-100 µl 721110
20-200 µl 721110
50-200 µl 721110
100-1000 µl 721120
200-1000 µl 721120
1-5 ml 721121
Secondary spring:
0.1-2.5 µl N/A
0.5-10 µl N/A
2-20 µl N/A
5-50 µl N/A
10-100 µl N/A
20-200 µl N/A
50-200 µl N/A
100-1000 µl 721463
200-1000 µl 721463
1-5 mI N/A
Tip cone:
0.1-2.5 µl 721012
0.5-10 µl 721011
2-20 µl 721020
5-50 µl 721020
10-100 µl 721030
20-200 µl 721030
50-200 µl 721030
100-1000 µl 721041
200-1000 µl 721041
1-5 mI 721047
Tip cone filter (pack of 50 pieces)
Standard Plus
0.1-2.5 µl N/A N/A
0.5-10 µl N/A N/A
2-20 µl 721008 721018
5-50 µl 721008 721018
10-100 µl 721007 721017
20-200 µl 721007 721017
50-200 µl 721007 721017
100-1000 µl 721006 721016
200-1000 µl 721006 721016
1-5 ml 721005 721015
Tip ejector collar:
0.1-2.5 µl 721053 (metal blue)
0.5-10 µl for 300 µl tip 721054 (metal blue)
0.5-10 µl for 300 µl tip 721755 (grey)
0.5-10 µl for 10 µl tip 721053 (metal blue)
0.5-10 µl for 10 µl tip 721754 (grey)
2-20 µl 721054 (metal blue)
5-50 µl 721054 (metal blue)
5-50 µl 721700 (red)
10-100 µl 721054 (metal blue)
20-200 µl 721054 (metal blue)
50-200 µl 721054 (metal blue)
50-200 µl 721806 (yellow)
100-1000 µl 721055 (metal blue)
200-1000 µl 721055 (metal blue)
200-1000 µl 721080 (blue)
1-5 mI 721056 (metal blue)
1-5 mI 721797 (green)
Tip ejector:
metal blue, 721057
grey, 721753
yellow, 721826
red, 721704
blue, 721230
green, 721793
Tip ejector spring:
721240
Push button:
metal blue, 721059
grey, 721751
white, 721223
yellow, 721824
red, 721703
blue, 721220
green, 721791
Counter:
0.1-2.5 µl 721185
0.5-10 µl 721182
2-20 µl 721192
5-50 µl 721191
10-100 µl 721202
20-200 µl 721201
50-200 µl 721201
100-1000 µl 721212
200-1000 µl 721212
1-5 mI 721213
N/A= Not Available
Grip cover:
metal blue, 721060
grey, 721752
yellow, 721825
red, 721706
blue, 721221
Handle, complete,
in gray colour:
4-channel:
5-50 µl 721070
50-250 µl 721071
8-channel:
0.5-10 µl 721072
5-50 µl 721073
50-300 µl 721075
12-channel:
0.5-10 µl 721076
5-50 µl 721077
50-300 µl 721079
Piston assembly:
0.5-10 µl 721276
5-50 µl 721271
50-250 µl 721272
50-300 µl 721278
Tip cone housing,
complete, in gray colour:
4-channel, 721677
8-channel, 721668
8-channel, 0.5-10 µl 721667
12-channel 721676
12-channel, 0.5-10 µl 721675
Tip ejector bar:
4-channel 721268
8-channel 721269
8-channel, 0.5-10 µl 721280
12-channel 721270
12-channel, 0.5-10 µl 721666
NOTE:
Coloured spare parts presented in
these two pages are for the metal
blue/gray Biohit Proline pipettors.
Spare part codes for the white
handles and tip cone housings are
in a separate spare part list, which
can be ordered from Biohit
subsidiaries or distributors.
Push button:
metal blue, 721059
grey, 721751
yellow, 721824
red, 721703
blue, 721220
Tip ejector, complete:
metal blue, 721058
grey, 721758
yellow, 721862
red, 721828
blue, 721231
Tip ejector spring:
721240
Tip cone:
0.5-10 µl 721275
5-50 µl 721273
50-250 µl 721274
50-300 µl 721277
Tip cone filter (set of 50 pcs):
0.5-10 µl N/A
5-50 µl 721014
50-300 µl 721014
Piston:
0.5-10 µl 721296
5-50 µl 721297
50-250 µl 721298
50-300 µl 721298
Piston spring:
0.5-10 µl 721261
5-50 µl 721261
50-250 µl 721261
50-300 µl 721261
Spring support:
0.5-10 µl 721262
5-50 µl 721262
50-250 µl 721262
50-300 µl 721262
Elastomer:
0.5-10 µl 721300
CONTENTS
1 . Your new Biohit Proline pipettor ..................................................... 34
1.1. Biohit Proline adjustable volume pipettors...................................34
1.2. Biohit Proline fixed volume pipettors ...........................................35
1.3. H-sign (Konformitätsbescheinigung) ............................................35
1.4. Biohit Proline tips ........................................................................ 36
2. Unpacking ......................................................................................... 37
3. Installing the pipettor holder ........................................................... 37
4. Pipettor materials ............................................................................ 38
5. Operating the pipettor ..................................................................... 39
5.1. Volume setting ............................................................................. 39
5.2. Sealing and ejecting tips ............................................................. 39
5.3. Two-in-one pipettors ................................................................... 40
5.4. Protective filters .......................................................................... 40
6. Pipetting techniques ........................................................................ 41
6.1. Forward pipetting ........................................................................ 41
6.2. Reverse pipetting ........................................................................ 42
7. Storage .............................................................................................. 42
8. Testing the performance and recalibration ................................... 42
8.1. Performance test (Checking calibration)...................................... 42
8.2. Recalibration procedure .............................................................. 44
9. Maintenance ...................................................................................... 44
9.1. Cleaning and decontaminating your pipettor ..............................44
9.2. In-house maintenance ................................................................. 45
10. Troubleshooting ................................................................................ 46
11. Warranty information .........................................................................47
33
1. Your new Biohit Proline pipettor
Your new Biohit Proline hand-held pipettor is a general purpose
pipettor, for the accurate and precise sampling and dispensing of liquid
volumes. All Biohit Proline pipettors operate on the basis of the air
displacement principle and use disposable tips.
The range of Biohit Proline pipettors cover a volume range from 0.1 µl
to 5 ml.
1.1. Blohit Proline adjustable volume pipettors
Single channel pipettors:
Multichannel pipettors:
Safe-Cone
Filters
50 pcs/bag
Cat.No. Volume Range Increment Tip Standard
720120 4-ch 5-50 µl 0.5 µl 300, 350 µl, 721014
350 µl 4 tip-band
720130 4-ch 50-250 µl 5 µl 300, 350 µl, 721014
350 µl 4 tip-band
720210 8-ch 0.5-10 µl 0.1 µl 10 µl 720220 8-ch 5-50 µl 0.5 µl 300, 350 µl, 721014
350 µl 4 tip-band
720240 8-ch 50-300 µl 5 µl 350 µl, 721014
350 µl 4 tip-band
720310 12-ch 0.5-10 µl 0.1 µl 10 µl 720320 12-ch 5-50 µl 0.5 µl 300, 350 µl, 721014
350 µl 4 tip-band
720340 12-ch 50-300 µl 5 µl 350 µl, 721014
350 µl 4 tip-band
34
Safe-Cone Filters
Volume 50 pcs/bag
Cat.No. Range Increment Tip Standard Plus
720005 0.1-2.5 µl 0.05 µl 10 µl - 720000 0.5-10 µl 0.1 µl 10, 300 µl - 720080 2-20 µl 0.5 µl 300 µl 721008 721018
720020 5-50 µl 0.5 µl 300, 350 µl 721008 721018
720050 10-100 µl 1 µl 300, 350 µl 721007 721017
720070 20-200 µl 1 µl 300, 350 µl 721007 721017
720030 50-200 µl 1 µl 300, 350 µl 721007 721017
720060 100-1000 µl 5 µl 1000 µl 721006 721016
720040 200-1000 µl 5 µl 1000 µl 721006 721016
720110 1000-5000 µl 50 µl 5000 µl 721005 721015
1.2. Biohit Proline fixed volume pipettors
Single channel pipettors:
1.3. H-sign (Konformitätsbescheinigung)
All Biohit Proline pipettors have been quality tested according to ISO
8655-6 and have received the H-sign (Konformitätsbescheinigung, DIN
12600) printed on the grip of each pipettor. The quality control
according to ISO 8655 involves gravimetric testing of each pipettor with
distilled water (grade 3, ISO 3696) at 22OC using the manufacturer’s
original tips.
35
Safe-Cone Filters
Volume 50 pcs/bag
Cat.No. Range Tip Standard Plus
722001 5 µl 10, 300, 350 µl - 722004 10 µl 10, 300, 350 µl - 722010 20 µl 300, 350 µl 721008 721018
722015 25 µl 300, 350 µl 721008 721018
722020 50 µl 300, 350 µl 721008 721018
722025 100 µl 300, 350 µl 721007 721017
722030 200 µl 300, 350 µl 721007 721017
722035 250 µl 1000 µl 721006 721016
722040 500 µl 1000 µl 721006 721016
722045 1000 µl 1000 µl 721006 721016
722050 2000 µl 5000 µl 721005 721015
722055 5000 µl 5000 µl 721005 721015
1.4. Biohit Proline tips
The full range of Biohit pipettor tips are recommended for use with
Biohit Proline pipettors. Biohit standard tips are made of virgin
polypropylene. Biohit also offers a full range of filter tips. Biohit
standard tips are available as bulk packages, space saving refill
systems and autoclavable (121°C, 20 min, 1 atm) trays. Pre-sterilized
tips in trays are also available. (Fig. 1.)
Fig. 1.
36
2. Unpacking
The Biohit Proline pipettor package contains the following items:
1. Pipettor
2. Calibration/Opening tool
3. Grease
4. Instructions for use
5. Pipettor holder
6. Tip
7. Identification stickers
8. Caps for colour coding (excludes fixed volume pipettors)
9. Filters (pipettors > 10 µl)
10. Performance certificate according to ISO 8655-6
3. Installing the pipettor holder
For convenience and safety always keep the pipettor vertically on its
own holder when not in use. When installing the holder, please follow
the instructions below:
1. Clean the shelf surface with ethanol.
2. Remove the protective paper from the adhesive tape
3. Install the holder as described in Figure 2A.
(Make sure the holder is pressed against the edge of the shelf.)
4. Place the pipettor onto the holder as shown in Figure 2B.
Fig. 2A
Fig. 3.
Fig. 2B
Note: An ergonomic, convenient carousel stand for 5 Biohit Proline
pipettors is also available (Cat. No.721000) (Fig. 3).
37
4. Pipettor Materials
Biohit Proline Single Channel
Biohit Proline Multichannel
38
Push button Push button
rod (ABS) (Operating button)
(PA)
Grip Cover
(PA) Tip ejector
(PA)
Handle
(ABS)
Display cover
(PC)
Tip Ejector
collar (PA)
Tip cone
2.5–1000 µl (PVDF)
2000–5000 µl (PPS)
Push button Push button
rod (ABS) (PA)
Grip cover
(PA) Tip Ejector
(PA)
Handle
(ABS)
Display cover
(PC)
Tip cone
housing
(ASA/PC)
Tip cone,
soft sealing
Tip ejector (PVDF)
bar (PETP) (TPU)
(PVDF)
Hard tip
cone
(PC)
39
5. Operating the pipettor
5.1. Volume setting
The volume of the pipettor is clearly shown through the handle display
window. The delivery volume (variable volume pipettors only) is set by
turning the operating button clockwise or anticlockwise (Fig. 4). When
setting the volume, please make sure that:
1. The desired delivery volume
clicks into place.
2. The digits are completely visible
in the display window.
3. The selected volume is within the
pipettor’s specified range.
Do not turn the operating button outside
the volume range because it may jam the
mechanism and damage the pipettor.
Fig. 4
5.2. Sealing and ejecting tips
Biohit Proline tips are recommended for use
with Biohit Proline pipettors. Before fitting a
tip ensure that the pipettor tip cone is clean.
Press the tip on the cone of the pipettor
firmly to ensure an airtight seal. The seal is
tight when a visible sealing ring forms
between the tip and the black tip cone
(Fig. 5).
Fig. 5
Each Biohit Proline pipettor is fitted with a
tip ejector to help eliminate the safety
hazards associated with contamination.
The tip ejector needs to be pressed firmly
downwards to ensure proper tip ejection
(Fig. 6).
Make sure that the tip is disposed of into a
suitable waste container.
Fig. 6
5.3. Two-in-one pipettors
The Biohit Proline fixed volume pipettors 5 and 10 µl, and variable
volume pipettors 0.5-10 µl can be used with both 10 µl and 300 µl tips.
The pipettors are supplied with two ejector collars and are factory fitted
with the ejector suitable for 10 µl tips. When using 300 µl tips, please
change the ejector collar by following the instructions below (Fig 7):
1.
3.
2.
Fig. 7
5.4. Protective filters
Fig. 8
Note: Change the filter on the tip cone regularly (after 50-250
pipettings).
40
The new tip cones of Biohit Proline
pipettors (>10 µl) allow the use of a
changeable filter as an option. The filter
prevents liquids and aerosols from
entering the pipettor. Two types of
filters are available: a Safe-Cone Filter
Standard and a Safe-Cone Filter Plus
(see cat.no. in Chapter 1.1). Plus filters
block the passage of liquids completely. The filters do not affect the calibration of the pipettor (Fig. 8).
1. Push the tip ejector down (1.).
2. Push the opening tool pin between
the ejector bar and ejector collar to
release the locking mechanism (2.).
3. Pull the ejector collar off (3.).
4. Install the 300 µl collar by pushing
the collar locking pin into the
ejector bar hole, keeping the tip
ejector pushed down.
5. Release the tip ejector and test
that it runs smoothly.
41
6. Pipetting techniques
1. Make sure that the pipettor, tips and the liquid are at the same
temperature.
2. Make sure that the tip is firmly attached to the tip cone.
3. Hold the pipettor vertically when aspirating the liquid and place
the tip only a few millimetres into the liquid.
4. Prerinse the tip before aspirating the liquid by filling and
emptying the tip 5 times. This is important especially when
dispensing liquids which have a viscosity and density different
from water.
5. Always control the operating button movements with the thumb
to ensure consistency.
6.1. Forward pipetting
This technique employs the blow-out function ensuring complete
delivery of the liquid.
1. Depress the operating button to the first stop (Fig. 9B).
2. Place the tip(s) just under the surface of the liquid (2-3 mm) and
smoothly release the operating button up to the starting
position (Fig.9A). Carefully withdraw the tip from the liquid,
touching against the edge of the container to remove excess.
3. Liquid is dispensed by gently depressing the operating button
to the first stop (Fig. 9B). After a short delay continue to
depress the operating button to the second stop (= blow-out)
(Fig 9C). This procedure will empty the tip(s) and ensure
accurate delivery.
4. Release the operating button to the starting position (Fig. 9A).
If necessary change the tip(s) and continue pipetting.
Starting position First stop Second stop
Fig. 9A Fig. 9B Fig. 9C
6.2. Reverse pipetting
A selected volume plus an excess is aspirated into the tip. The delivery
is done without blow-out, and so the excess volume remains in the tip.
The reverse technique is recommended for high viscosity, biological or
foaming liquids, or very small volumes of liquid.
1. Depress the operating button all the way to the second stop
(Fig. 9C). Place the tip(s) just under the surface of the liquid
(2-3 mm) and smoothly release the operating button up to the
starting position (Fig.9A).
2. Withdraw the tip(s) from the liquid touching against the edge of
the container to remove excess.
3. Deliver the preset volume by smoothly depressing the
operating button to the first stop (Fig. 9B). Hold the operating
button at the first stop. The liquid that remains in the tip(s)
should not be included in the delivery.
4. The remaining liquid should now be discarded with the tip(s) or
delivered back into the container vessel.
7. Storage
When not in use it is recommended that your Biohit Proline pipettor is
stored in a vertical position. See Installing the pipettor holder (Chapter
3).
8. Testing the performance and recalibration
It is recommended to check the performance of your Biohit Proline
pipettors regularly (e.g. every 3 months) and always after in-house
maintenance. However, the user should establish a regular testing
routine for their pipettors with regard to accuracy requirements of the
application, frequency of use, number of operators using the pipettor,
nature of the liquid dispensed and the acceptable maximum
permissible errors established by the user. (ISO 8655-1.)
8.1. Testing the performance
Performance testing should take place in a draught-free room at 15 –
30OC, constant to +/- 0.5OC and humidity above 50%. The pipettor, tips
and the test water should have stood in the test room a sufficient time
(at least 2 hours) to reach equilibrium with the room conditions. Use
distilled or deionised water (grade 3, ISO 3696). Use an analytical
balance with a readability of 0.01 mgs. (ISO 8655-6.)
Weighing
1. Adjust the desired test volume V
s
.
2. Carefully fit the tip onto the tip cone.
3. Fill the tip with test water and expel to waste five times to reach a
humidity equilibrium in the dead air volume.
4. Replace the tip. Pre-wet the tip by filling it once with test water and
expel to waste.
5. Aspirate the test water, immersing the tip only 2-3 mm below the
surface of the water. Keep the pipettor vertical.
6. Withdraw the pipettor vertically and touch the tip against the inside
wall of the test water container.
42
7. Pipette the water into the weighing vessel, touching the tip against
the inside wall of the vessel just above the liquid surface at an
angle of 30° to 45°. Withdraw the pipettor by drawing the tip 8-10
mm along the inner wall of the weighing vessel.
8. Read the weight in mgs (m
i
).
9. Repeat the test cycle until 10 measurements have been recorded.
10. Convert the recorded masses (m
i
) to volumes (Vi)
V
i
= miZ Z = correction factor (Table 1)
11. Calculate the mean volume (V) delivered:
V = (∑V
i
)/10
12. For conformity evaluation calculate the systematic error e
s
of the
measurement:
in µl: e
s
= V – V
s
Vs= selected test volume
or in %: es= 100 (V – Vs)/V
s
13. For conformity evaluation calculate the random error of the
measurement:
as standard deviation s = ∑(V
i
– V)
2
n = number of
measurement (10)
n – 1
or as coefficient of variation CV = 100S/V
14. Compare the systematic error (inaccuracy) and random error
(imprecision) with the values in the performance specifications
(pages 48, 49), or the specifications of your own laboratory.
If the results fall within the specifications, the pipettor is ready for
use. Otherwise check both systematic and random errors and,
when necessary, proceed to the recalibration procedure
(Chapter 8.2).
Note: Systematic error (inaccuracy) is the difference between the
dispensed volume and the selected test volume. Random error
(imprecision) is the scatter of the dispensed volumes around the mean
of the dispensed volume. (ISO 8655-1.)
Note: Biohit specifications are achieved in strictly controlled conditions
(ISO 8655-6). The user should establish own specifications based on
the field of use and the accuracy requirements placed on the pipettor
(ISO8655-1).
Table 1
Note: This method is based on ISO 8655.
43
Z-values (µl/mg):
Temp. (°C) Air Pressure (kPa)
95 100 101.3 105
20.0 1.0028 1.0028 1.0029 1.0029
20.5 1.0029 1.0029 1.0030 1.0030
21.0 1.0030 1.0031 1.0031 1.0031
21.5 1.0031 1.0032 1.0032 1.0032
22.0 1.0032 1.0033 1.0033 1.0033
22.5 1.0033 1.0034 1.0034 1.0034
23.0 1.0034 1.0035 1.0035 1.0036
23.5 1.0036 1.0036 1.0036 1.0037
8.2. Recalibration procedure
+
–
Fig. 10
9. Maintenance
To maintain the best results from your Biohit Proline pipettor each unit
should be checked every day for cleanliness. Particular attention
should be paid to the tip cone(s).
Biohit Proline pipettors have been designed for easy in-house service.
However, Biohit also provides complete repair and recalibration service
including a service report and performance certificate(s). Please return
your pipettor to your local representative for repair or performance test/
recalibration. Before returning please make sure that it is free from all
contamination. Please advise our Service Representative of any
hazardous materials which may have been used with your pipettor.
Note: Check the performance of your Biohit Proline pipettor regularly
e.g. every 3 months and always after in-house service or maintenance.
9.1. Cleaning and decontaminating your pipettor
To clean and decontaminate your pipettor, lightly spray the outside of
the pipettor with Biohit Proline Biocontrol decontamination solution
(Cat. No. 724004, 5 l) or use ethanol. Wipe dry with a soft cloth or lintfree tissue. It is recommended to clean and decontaminate the tip cone
regularly.
1. Place the calibration tool into
the holes of the calibration
adjustment lock (under the
operating button) (Fig. 10).
2. Turn the adjustment lock
anticlockwise to decrease
and clockwise to increase
the volume.
3. Repeat Performance test
(Chapter 8.1.) procedure
from step 1 until the pipetting
results are correct.
44
45
9.2. In-house maintenance
1. Hold down the tip ejector.
2. Place the tooth of the opening tool between the tip ejector and
the tip ejector collar to release the locking mechanism (Fig. 11).
3. Carefully release the tip ejector and remove the tip ejector
collar.
4. Place the wrench end of the opening tool over the tip cone,
turning it anticlockwise. Do not use any other tools (Fig. 12).
The 5 ml tip cone is removed by turning it anticlockwise without
any tools (Fig. 13).
5. Remove the tip cone, piston, and spring. Remove the filter if
fitted.
6. Place the tip cone, tip ejector, tip ejector collar, piston, O-ring,
and spring into a beaker containing Biohit Proline Biocontrol
and leave for at least 30 minutes to secure complete
decontamination.
7. Remove the components from the beaker and rinse them with
distilled water, then dry, preferably with warm air, for at least
one hour.
8. Before replacing the tip cone it is recommended to grease the
piston and O-ring slightly by using the silicone grease provided.
9. After reassembling press the operating button several times to
ensure that the grease has spread evenly.
10.Check the pipettor calibration.
Note: Instead of Biocontrol you can use ethanol: Wipe the piston, the
O-ring, and the tip cone with ethanol and a lint-free cloth.
Note: On models 720005, 720000, 722001 and 722004 (≤10 µl
pipettors) the O-ring cannot be accessed for maintenance.
Note: Excessive use of grease may jam the piston.
1.
1.
1.
3.
2. 2. 2.
Fig. 11 Fig. 12 Fig. 13
10. Troubleshooting
Trouble Possible cause Solution
Droplets left Unsuitable tip Use original Biohit tips
inside the tip
Non-uniform wetting Attach new tip
of the tip plastic
Leakage or Tip incorrectly attached Attach firmly
pipetted volume
too small Unsuitable tip Use original Biohit tips
Foreign particles Clean the tip cone,
between tip and tip attach new tip
cone
Instrument Clean and grease
contaminated O-ring and piston,
clean the tip cone (*)
Insufficient amount of Grease accordingly
grease on piston and
O-ring
O-ring not correctly Change the O-ring
positioned or damaged
Incorrect operation Follow instructions
carefully
Calibration altered or Recalibrate according
unsuitable for the liquid to instructions
Instrument damaged Send for service
Push button Piston contaminated Clean and grease
jammed or O-ring and piston,
moves erratically clean the tip cone (*)
Penetration of solvent Clean and grease
vapours O-ring and piston,
clean the tip cone (*)
Pipettor blocked, Liquid has penetrated Clean and grease
aspirated volume tip cone and dried O-ring and piston,
too small clean the tip cone (*)
Tip ejector Tip cone and/or ejector Clean the tip cone and
jammed or collar contaminated the ejector collar (*)
moves
erratically
(*) Clean with Biohit Proline Biocontrol or 75% ethanol.
46
11. Warranty information
Biohit Proline pipettors are covered by warranty for 3 years against
defects in materials and workmanship. Should your Proline pipettor fail
to function at any time, please contact your local Biohit representative.
ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT
IS FOUND TO HAVE BEEN CAUSED BY MALTREATMENT, MISUSE,
UNAUTHORIZED MAINTENANCE OF SERVICE OR NEGLIGENCE
OF REGULAR MAINTENANCE AND SERVICE, ACCIDENTAL
DAMAGE, INCORRECT STORAGE OR USE OF THE PRODUCTS
FOR OPERATIONS OUTSIDE THEIR SPECIFIED LIMITATIONS,
OUTSIDE THEIR SPECIFICATIONS, CONTRARY TO THE
INSTRUCTIONS GIVEN IN THIS MANUAL OR WITH OTHER THAN
THE MANUFACTURER’S ORIGINAL TIPS.
Each Biohit Proline pipettor is tested before shipping by the
manufacturer. The Biohit Quality Assurance Procedure guarantees that
the Biohit Proline pipettor you have purchased is ready for use.
47
Biohit Proline Multichannel Adjustable Volume Pipettors
Inaccuracy Imprecision
Cat.No. Volume range Volume ± ±
720120 4-ch 5-50 µl 50 µl 1.00% 0.50%
25 µl 1.50% 1.00%
5 µl 3.00% 2.00%
720130 4-ch 50-250 µl 250 µl 0.70% 0.25%
125 µl 1.00% 0.50%
50 µl 1.50% 0.80%
Specifications
Biohit Proline Single Channel Adjustable Volume Pipettors
Volume Inaccuracy Imprecision
Cat.No. range Volume ± ±
720005 0.1-2.5 µl 2.5 µl 2.50% 2.00%
1.25 µl 3.00% 3.00%
0.25 µI 12.00% 6.00%
720000 0.5-10 µl 10 µl 1.00% 0.80%
5 µl 1.50% 1.50%
1 µl 2.50% 1.50%
700080 2-20 µl 20 µl 0.90% 0.40%
10 µl 1.20% 1.00%
2 µl 3.00% 2.00%
720020 5-50 µl 50 µl 0.60% 0.30%
25 µl 0.90% 0.60%
5 µl 2.00% 2.00%
700050 10-100 µl 100 µl 0.80% 0.15%
50 µl 1.00% 0.40%
10 µl 3.00% 1.50%
720070 20-200 µl 200 µl 0.60% 0.15%
100 µl 0.80% 0.30%
20 µl 3.00% 1.00%
720030 50-200 µl 200 µl 0.60% 0.15%
100 µl 0.80% 0.30%
50 µl 1.00% 0.40%
720060 100-1000 µl 1000 µl 0.60% 0.20%
500 µl 0.70% 0.25%
100 µl 2.00% 0.70%
720040 200-1000 µI 1000 µI 0.60% 0.20%
500 µI 0.70% 0.25%
200 µI 0.90% 0.30%
720110 1-5 ml 5 ml 0.50% 0.15%
2.5 ml 0.60% 0.30%
1 ml 0.70% 0.30%
48
Inaccuracy Imprecision
Cat.No. Volume range Volume ± ±
720210 8-ch 0.5-10 µl 10 µl 1.50% 1.50%
5 µl 2.50% 2.50%
1 µl 4.00% 4.00%
720220 8-ch 5-50 µl 50 µl 1.00% 0.50%
25 µl 1.50% 1.00%
5 µl 3.00% 2.00%
720240 8-ch 50-300 µl 300 µl 0.70% 0.25%
150 µl 1.00% 0.50%
50 µl 1.50% 0.80%
720310 12-ch 0.5-10 µl 10 µl 1.50% 1.50,%
5 µl 2.50% 2.50%
1 µl 4.00% 4.00%
720320 12-ch 5-50 µl 50 µl 1.00% 0.50%
25 µl 1.50% 1.00%
5 µl 3.00% 2.00%
720340 12-ch 50-300 µl 300 µl 0.70% 0.25%
150 µl 1.00% 0.50%
50 µl 1.50% 0.80%
Biohit Proline Fixed Volume Pipettors
Inaccuracy Imprecision
Cat.No. Volume range Volume ± ±
722001 5 µl 5 µl 1.30% 1.20%
722004 10 µl 10 µl 0.80% 0.80%
722010 20 µl 20 µl 0.60% 0.50%
722015 25 µl 25 µl 0.50% 0.30%
722020 50 µl 50 µl 0.50% 0.30%
722025 100 µl 100 µl 0.50% 0.30%
722030 200 µl 200 µl 0.40% 0.20%
722035 250 µl 250 µl 0.40% 0.20%
722040 500 µl 500 µl 0.30% 0.20%
722045 1000 µl 1000 µl 0.30% 0.20%
722050 2000 µl 2000 µl 0.30% 0.15%
722055 5000 µl 5000 µl 0.30% 0.15%
Liquid: Distilled water (grade 3, ISO 3696)
Reference temperature: 22
O
C. constant to ± 0.5OC
Tested: According to ISO 8655 using original
Biohit Proline tips
Loading...