Biohit mLINE m3, mLINE m10, mLINE m20, mLINE m100, mLINE m1000 Instruction Manual

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mLINE

®

Instruction Manual

Bedienungsanleitung

Mode d’ emploi

Manual Usuario

Instruzioni d’impiego

Инструкция пользователя

m3 m10 m20 m100 m200 m1000 m5000 m10 ml
1 726051 726051 726052 726052 726052 726056 726054 726055
2 726041 726041 726041 726041 726041 726042 N/A N/A
3 726044 731084 726083 726042 726039 726048 N/A N/A
4 N/A N/A N/A N/A N/A N/A 726038 726047
5 726032 731076 726084 726034 726035 726036 726037 726031
6 N/A N/A N/A N/A N/A N/A N/A N/A
7 726006 726006 726006 726006 726006 726006 726006 726006
8 726021 726022 726082 726026 726027 726028 726029 726012
9 726012 726012 726012 726012 726012 726012 726012 726012
10 726005 726005 726005 726005 726005 726005 726005 726005
11 726004 726004 726004 726004 726004 726004 726004 726004
12 726003 726003 726003 726003 726003 726003 726003 726003
13 726002 726002 726002 726002 726002 726002 726002 726002
14 726001 726001 726001 726001 726001 726001 726001 726001

14

13

11

12

10

9

7

8

3

5

1

2

m3, m10,
m20, m100

m200, m1000 m5000, m10 ml

14

13

11

12

10

9

7

8

3

5

1

2

14

13

11

12

10

9

7

8

5

1

6

4

6

Spare Parts

1 Tip ejector

collar

2 Tip cone

holder

3 Tip cone

complete

4 Tip cone

cylinder

5 Piston
6 Piston seal

7 Calibration

nut cover

8 Display
9 Handle
10 Tip ejector

11 ID window

12 ID tag

13 Handle collar

14 Cap (set of

5 colours)

Ersatzteile

Spitzenabwurf

Konusfilterhalter

Spitzenkonus

Konuszylinder

Kolben
Kolbenstempel

Abdeckung
Kalibrierschraube

Display
Handgriff
Spitzenabwerfer

ID Fenster

ID Aufkleber

Griffhalter

Abdeckungen
(5 Farben Set)

Piéces détachées

Tube d’éjection

Bague de retenue

Porte-cône
complet

Cylindre

Piston
Joint de piston

Dessus d’écrou

Affichage
Ejecteur
Corps de pipette

Corps d’identification

Clip d’identification

Poignet

Bouchons
(5 couleurs)

Repuestos

Expulsor de puntas

Anillo soporte de
puntas

Soporte de puntas
completo

Cilindro del soporte
de puntas

Pistón
Junta de pistón

Tapa del mecanismo
de calibración

Display
Empuñadura
Sistema expulsor

de puntas
Visor ID

Etiqueta de
identificación

Cubierta de la
empuñadura

Tapas
(set de 5 colores)

Parti di ricambio

Collare dell’espulsore
puntale

Contenitore cono

Cono ancoraggio
puntali completo

Cilindro cono

Pistone
Sigilla pistone

Copri dado di
calibrazione

Display
Impugnatura
Espulsore puntali

Finestra ID

Etichetta ID

Collare
dell’impugnatura

Cappuccio
(set di 5 colori)

Запасная часть

Сбрасыватель
наконечников

Муфта крепления
посадочного конуса

Посадочный конус
в сборе

Посадочный конус

Поршень

Уплотнительна я
манжета

Узел калибровки

Крышка дисплея

Рукоять

Сбрасыватель
наконечников

Прозрачная
защитная пластинка

Идентификационный
вкладыш

Воротник рукояти

Декоративный
съемный колпачок
(5 цветов)

m10/8 m100/8 m300/8 m10/12 m100/12 m300/12
1 726131 726132 726133 726134 726135 726136
2 726137 726138 726138 726139 726140 726140
3 726149 726150 726151 726149 726150 726151
4 726146 726147 726148 726146 726147 726148
5 726152 726153 726154 726152 726153 726154
6 726006 726006 726006 726006 726006 726006
7 726022 726141 726142 726022 726141 726142
8 726012 726012 726012 726012 726012 726012
9 726005 726005 726005 726005 726005 726005
10 726004 726004 726004 726004 726004 726004
11 726003 726003 726003 726003 726003 726003
12 726002 726002 726002 726002 726002 726002
13 726001 726001 726001 726001 726001 726001

8-channel: m10, m100, m300
12-channel:

m10, m100, m300

13

11

12

10

9

7

8

3

5

1

2

6

4

Spare Parts

1 Tip cone

housing

2 Tip ejector bar

3 Tip cone

complete
4 Piston
5 Piston

assembly
6 Calibration

nut cover
7 Display
8 Handle
9 Tip ejector

10 ID window

11 ID tag

12 Handle collar

13 Cap

(set of 5

colours)

Ersatzteile

Spitzenkonus­gehäuse

Spitzenabwurfs­balken

Spitzenkonus

Kolben
Kolbenzu-

sammensetzung
Abdeckung

Kalibrierschraube
Display
Handgriff
Spitzenabwerfer

ID Fenster

ID Aufkleber

Griffhalter

Abdeckungen
(5 Farben Set)

Piéces détachées

Module
porte-cônes

Barre d’éjection

Porte-cône
complet

Piston
Piston

assemblé
Dessus d’écrou

Affichage
Ejecteur
Corps de pipette

Corps
d’identification

Clip d’identification

Poignet

Bouchons
(5 couleurs)

Repuestos

Carcasa del
Soporte de puntas

Expulsor de
puntas

Soporte de puntas
completo

Pistón
Conjunto de

pistón
Tapa del mecanismo

de calibración
Display
Empuñadura
Sistema expulsor

de puntas
Visor ID

Etiqueta de
identificación

Cubierta de la
empuñadura

Tapas
(set de 5 colores)

Parti di ricambio

Alloggiamento del
cono di ancorag­gio del puntale

Barra di espul­sione del puntale

Cono ancoraggio
puntali completo

Pistone
Assemblaggio

del pistone
Copri dado di

calibrazione
Display
Impugnatura
Espulsore puntali

Finestra ID

Etichetta ID

Collare
dell’impugnatura

Cappuccio
(set di 5 colori)

Запасная часть

Корпус поршневой
части

Сбрасыватель
наконечника

Посадочный конус

Поршень

Поршень системы
в сборе

Узел калибровки

Крышка дисплея

Рукоять

Сбрасыватель
наконечников

Прозрачная
защитная пластинка

Идентификационный
вкладыш

Воротник рукояти

Декоративный
съемный колпачок
(5 цветов)

Instruction Manual .......................................1

Bedienungsanleitung .................................. 17

Mode d’emploi ...........................................33

Manual Usuario ..........................................49

Instruzioni d’impiego ..................................65

Инструкция пользователя .............................. 81

Specifications ............................................. 97

mLINE

®

1

CONTENTS

1. INTENDED USE ........................................................... 2

2. PRODUCT DESCRIPTION .......................................... 2

3. BIOHIT OPTIFIT TIPS .................................................. 4

4. UNPACKING ................................................................ 4

5. PERSONAL IDENTIFICATION .................................... 4

6. PIPETTE HOLDER AND CAROUSEL STAND ........... 5

6.1. Installing the pipette holder ................................ 5

6.2. Carousel stand ................................................... 5

7. CALIBR ATION TOOL

(also tube and ID window opener) ............................ 5

8. OPERATING THE PIPETTE ........................................ 6

8.1. Setting the volume .............................................. 6

8.2. Sealing and ejecting tips .................................... 6

8.3. Protective tip cone fi lters .................................... 7

9. PIPETTING TECHNIQUES .......................................... 7

9.1. Forward pipetting (Fig. 5) ................................... 8

9.2. Reverse pipetting (Fig. 9).................................... 8

9.3. Repetitive reverse technique .............................. 9

10. RECOMMENDATIONS FOR GOOD PIPETTING ...... 9

11. MAINTENANCE ........................................................... 9

11.1. Daily cleaning of the outer surface

of the pipette ..................................................... 10

11.2. Cleaning and decontaminating

the lower part of the pipette ............................. 10

11.3. Sterilization of the pipette .................................11

12. TESTING THE PERFORMANCE AND

RECALIBRATION ....................................................... 12

12.1. Testing the performance .................................... 12

12.2. Recalibration ..................................................... 14

13. TROUBLE SHOOTING .............................................. 15

14. WARRANTY INFORMATION .................................... 16

15. PERFORMANCE SPECIFICATIONS ........................ 16

2

1. INTENDED USE

This liquid handling device is designed and manufactured to be
used with the IVD-product as an IVD-accessory and as a stand
alone laboratory equipment.

2. PRODUCT DESCRIPTION

Your new Biohit mLINE is an autoclavable air displacement
pipette. The attractive and ergonomical design of the mLINE
pipette together with its very low pipetting forces reduce the risk
of repetitive strain injuries (RSI)1. The pipette is designed for both
right- and left-handeduse

The special tip cone design of the pipette offers the possibility to
use replaceable Safe-Cone fi lters to help prevent the contamination
and damage of the pipette. The operating button of the mLINE
allows safe and easy fi lter ejection. The pipette uses disposable
tips.

Biohit mLINE single- and multichannel pipettes

1.

Note: It is knowledge that prolonged pipetting can cause RSI.
The manufacturer is not responsible for RSI or any related diseases
caused by prolonged pipetting.

Safe Cone fi lters
Colour Volume 50 pcs/bag
Cat. No. Code Channels Range Increment Tip Standard Plus

725010 Grey 1-ch 0.1 - 3 μl 0.002 μl 10 N/A N/A
725020 Grey 1-ch 0.5 - 10 μl 0.01 μl 10 N/A N/A
725030 Yellow 1-ch 2 - 20 μl 0.02 μl 300 721014 N/A
725050 Yellow 1-ch 10 - 100 μl 0.1 μl 300, 350 721008 721018
725060 Yellow 1-ch 20 - 200 μl 0.2 μl 300, 350 721007 721017
725070 Blue 1-ch 100 - 1000 μl 1 μl 1000 721006 721016
725080 Green 1-ch 500 - 5000 μl 10 μl 5000 721005 721015
725090 Red 1-ch 1 - 10 ml 20 μl 10 ml 721005 721015

725120 Grey 8-ch 0.5 - 10 μl 0.01 μl 10 N/A N/A
725130 Yellow 8-ch 5 - 100 μl 0.1 μl 300, 350 721008 721018
725140 Orange 8-ch 30 - 300 μl 0.2 μl 350 721007 721017

725220 Grey 12-ch 0.5 - 10 μl 0.01 μl 10 N/A N/A
725230 Yellow 12-ch 5 - 100 μl 0.1μl 300, 350 721008 721018
725240 Orange 12-ch 30 - 300 μl 0.2 μl 350 721007 721017

3

Pipette Description

Cap .......................

Operating

button ...................

Tip

ejector .......

Display .............

Volume

lock ..................

Colour

coding...............

Tip ejector

collar ......................

Tip cone....................

ID window
and tag

Finger
support

........ Handle

... Calibration
nut

Safe Cone

....... fi lter

Tip cone ...............

........ Tip cone

housing

Tip

........ ejector bar

........ Safe Cone

fi lter

4

3. BIOHIT OPTIFIT TIPS

The full range of Biohit pipette tips are recommended for use with
Biohit mLINE pipettes. Biohit standard tips are made of virgin
polypropylene. Biohit also offers a full range of Safetyspace fi lter
tips. Biohit standard tips are available as bulk packages, space
saving refi ll systems and autoclavable (121°C, 20 min, 1 atm) trays.
Pre-sterilized tips in trays are also available. (Fig. 1.)

Fig. 1.

4. UNPACKING

The Biohit mLINE pipette package contains the following items:

1. Pipette

2. Tip

3. Caps for colour coding

4. Calibration tool (also tube and ID window opener)

5. Pipette holder

6. Identifi cation tags

7. Safe-Cone fi lters (pipettors >10 μl)

8. Grease

9. Instruction manual

10. Performance certifi cation in accordance with ISO 8655-6

Please check that all items are included and that no damage has
occurred during shipment.

5. PERSONAL IDENTIFICATION

Each Biohit mLINE pipette can be identifi ed with a tag located
underthe ID window which is placed on the fi nger support.

1.

Remove the ID window by using the calibration tool (Chapter 7.).

2. Remove the tag and mark the identifying information on it.

3. Position the tag on place and clip the window back into

place.

5

A

B

C

6. PIPETTE HOLDER AND CAROUSEL STAND

For convenience and safety always keep the pipette vertically on
its own holder, carousel or linear stand when not in use.

Cat.No. Product
725600 Biohit mLINE Carousel
725610 Biohit mLINE Pipette Holder

6.1. Installing the pipette holder

1. Clean the shelf surface with ethanol.

2. Remove the protective paper from

the adhesive tape.

3. Install the holder by pressing it

against the edge of the shelf (Fig. 2).

4. Place the pipette onto the holder.

6.2. Carousel stand

A convenient and ergonomical
Carousel Stand (Fig. 3) for six
Biohit mLINE pipettes is also available

7. CALIBR ATIO N TOOL
(also tube and ID window opener)

The calibration tool is designed for the following purposes (Fig. 4):

1. Tool for recalibration (A).

2. Opening tool for the ID window and lid of
the calibration nut (B).

3. Opening tool for the tubes (C).

Fig. 4.

Fig. 2.

Fig. 3.

6

8. OPERATING THE PIPETTE

8.1. Setting the volume

The volume of the pipette is clearly shown through the display. The
volume setting is carried out with the following steps (Fig. 5):

1. Press the volume lock and hold it down.

2. Set the volume by rotating the operating button (clockwise to

decrease the volume and counterclockwise to increase).

3. Release the volume lock.

Fig. 5. Volume Lock

+

–

Note: Do not rotate the operating button without pressing down
the volume lock. Do not rotate the operating button outside the
volume range of the pipette (Chapter. 1.)

8.2. Sealing and ejecting tips

To ensure the maximum accuracy and precision Biohit tips are
recommended to be used with mLINE pipettes. Before fi tting a tip
ensure that the pipette tip cone is clean. Press the tip on the tip
cone of the pipette. The pipette is provided with a spring loaded
tip conefor optimal sealing.

Eject the tip by pressing the tip ejector with your thumb (Fig. 6).
Make sure that the tip is disposed into a suitable waste container.

Fig. 4. Tip ejector

Note: If the tip is pressed too hard the tip cone yields and, as a
result, the counter and operating button moves.

7

8.3. Protective tip cone fi lters

The tip cone design of the Biohit mLINE pipettes (>10 μl) allows the
use of Biohit Safe-Cone fi lters in the tip cones. These removable
fi lters prevent liquids and aerosols from entering the pipette and,
thus, protects the pipette from contamination and damage.

The Safe-Cone fi lters are available as Standard or Plus versions. It
is recommended to use the Standard fi lter for general applications
and the Plus fi lter for more demanding applications such as cell
culture, bacterial and virological work and molecular biology.
Filters need to be changed regularly. The interval for fi lter changing
is application dependant but the recommendation is to change the
fi lter daily (after 50 - 250 pipetting cycles) and always in case of
over-aspiration. (See Chapter 2 for ordering information.)

The Safe-Cone fi lters can be removed with the unique fi lter ejector
without touching the fi lters by hand. Remove the operating button
cap and eject the fi lter by pressing the operating button down
(Fig. 7). Make sure that the fi lter is disposed into a suitable waste
container. Clean the tip cone if needed and put the new fi lter in
place.

Fig. 7.

9. PIPETTING TECHNIQUES

Two basic pipetting techniques, forward and reverse pipetting,
are associated with mLINE pipettes. Forward pipetting is the
most common used pipetting technique. The technique employs
the blow-out function ensuring complete delivery of the liquid.
Reverse pipetting is recommended for highly viscous, biological
or foaming liquids, or very small volumes of liquid. A selected
volume plus an excess is aspirated into the tip. The deliver y is done
without blow-out, and, thus, the excess volume remains in the tip.
The reverse technique also facilitates the repeated delivery of the
same volume. If reverse pipetting technique is used the pipette
might need recalibration.

8

9.1. Forward pipetting (Fig. 5)

1. Fit the tip onto the pipette tip cone.

2. Press the operating button to the fi rst stop.

3. Place the tip just under the surface of the liquid (2-3 mm)

and smoothly release the operating button up to the starting
position.Wait one second. Carefully withdraw the tip from the
liquid, touching against the edge of the container to remove
excess fromthe outside of the tip.

4. Dispense the liquid by pressing the operating button to the

fi rst stop. After a short delay press the operating button to the
second stop. This action will empty the tip.

Fig. 8. 0

1
2

9.2. Reverse pipetting (Fig. 9)

1. Fit the tip onto the pipette tip cone.

2. Press the operating button all the way to the second stop.

3. Place the tip just under the surface of the liquid (2-3 mm)

and smoothly release the operating button up to the starting
position.

4. Withdraw the tip from the liquid touching against the edge of

the container to remove excess.

5. Press the operating button smoothly to the fi rst stop to deliver

the present volume. Hold the operating button at the fi rst stop.
The liquid that remains in the tip should not be included in the
delivery.

6. Discard the remaining liquid by pressing the operating button

to the second stop.

Fig. 9. 0

1
2

9

9.3. Repetitive reverse technique

1. Follow the reverse technique steps 1 to 5.

2. Continue pipetting by repeating steps 3 to 5 as long as

needed.

3. Finally discard the remaining liquid by pressing the operating

button to the second stop.

10. RECOMMENDATIONS FOR GOOD PIPETTING

• Make sure that the tip is fi rmly attached to the tip cone.

• Hold the pipettor vertically when aspirating the liquid and place

the tip only a few millimeters into the liquid.

• Always control the operating button slowly and smoothly.

• Pre-rinse the tip before aspirating the liquid by fi lling and

emptying the tip for three to fi ve times. This is important
especially when pipetting liquids with a viscosity and density
greater than water or liquids with high vapor pressure (e.g.
ethanol).

• Check that the pipette, tip and liquid are at the same

temperature.

• When pipetting liquids with temperatures different to the

ambient temperature change the tip after each pipetting. Do
not pre-rinse the tip.

• To avoid contamination, do not rest the pipette on its side

especially with the tip attached.

• Change the tip cone fi lter regularly (recommendation after 50

-250 pipetting cycles).

• Never strike the tip cone against the tip tray when mounting the

tip as this can damage the pipette.

• Avoid rough handling and do not drop the pipette.

• Avoid exposing the unit to extreme temperature changes,

humidity and dust (operating temperature from 15°C to 40°C).

11. MAINTENANCE

Biohit mLINE pipettes have been designed for easy in-house
service. If the pipette is used daily it is recommended to clean/
decontaminate and check the performance of the pipette every
th re e m ont hs . B io hit al so pr ov ide s c omp le te re pa ir a nd re cal ib rat io n
service for your pipette including a service report and performance
certifi cate.

Note: Please make sure that the pipette has been decontaminated
before you send it to us or your local representative. Please advise
if any hazardous material has been used with your pipette.

Note: The use of the tip cone fi lters may prolong the interval of the
service. Change the fi lters regularly.

Note: It is recommended to always use gloves when cleaning the
pipette.

10

11.1. Daily cleaning of the outer surface of the pipette

Your Biohit mLINE pipette should be checked every day for
cleanliness. To clean and decontaminate the outer surface of your
pipette use Biohit Proline Biocontrol (Cat.no. 724004, 5 litres)
and a soft lint-free cloth. It is also possible to use ethanol (70%),
isopropanol (60%) or a mild detergent as a cleaning agent.

Gently clean the surface of the pipette with moistened cloth and
wipe dry. Pay special attention to the tip cone. Change the tip cone
fi lter if needed (Chapter 8.3.)

11.2. Cleaning and decontaminating the lower part
of the pipette

If your pipette is in daily use it is recommended to clean/
decontaminate and grease the pipette every three months. It is
recommended to send the multichannel pipettes to your local
Biohit service representative for cleaning and greasing. To clean
and decontaminate the lower parts of the single channel pipette,
follow these steps:

Disassembling and cleaning
(see the pictures under the cover page):

1. Eject the tip cone fi lter (if fi tted, Chapter 8.3.)

2.

Unscrew the tip ejector collar (1) counterclockwise and remove it.

3. Pipettes m3, m10, m20, m100, m200, m1000:
Unscrew the tip cone holder (2) counterclockwise and carefully

remove it with the tip cone (3).

Pipettes m5000 and m10 000:

Unscrew the tip cone cylinder (4) counterclockwise and remove it.

4. Clean the tip ejector collar, the tip cone holder, the tip cone

(cylinder) and the piston (5) with Biohit Proline Biocontrol,
ethanol (70%), isopropanol (60%) or mild detergent and soft
lint-free cloth.

5. Clean the interior of the tip ejector collar and the tip cone

(cylinder) with a cotton swab. Be careful with the pipettes m3,

m10, m20 and m100 so that the seal inside the tip cone will not

damage.

6. Rinse the parts with distilled water if needed and let the parts

dry.

7. Pipe t t e s m3, m10, m20 and m100:
Put a thin layer of grease on the piston (5).
Pipettes m200 and m1000:
Put a thin layer of grease around the seal (6).
Pipettes m5000 and m10 000:
Put a thin layer of grease on the seal (6) and the interior of the

tip cone cylinder (4).

Note: Avoid excess grease. Use only the grease provided with
the pipette.

Note: Before reassembling check that no lint or particles are on
the surface of the piston.

11

Decontamination:
For complete decontamination of the lower parts place the tip
ejector collar (1), tip cone holder (2), tip cone (3) and tip cone
cylinder (4) (only the models m5000 and m10 000) into a beaker
containing Biohit Proline‚ Biocontrol and leave for at least 30
minutes. Wipe the piston with Biohit Proline‚ Biocontrol and the
lint-free cloth. Rinse the parts with distilled or sterile water. Let the
parts dry. Grease the piston and seal according to the instructions
given earlier in this chapter.

Reassembling:

1. Pipettes

m3, m10, m20, m100, m200, m1000:

Carefully place the tip cone (3) on the piston and attach it by

screwing the tip cone holder (2) clockwise.
Pipettes m5000 and m10 000:
Carefully place the tip cone cylinder (4) on the piston and screw

clockwise. Make sure that the tip cone (cylinder) is properly

tightened. Avoid over tightening.

2. Attach the tip ejector collar (1) by screwing it clockwise.

3. Place the new tip cone fi lter on its place.

4. Press the operating button several times to ensure that the

grease has spread evenly.

5. Check the performance of the pipette.

Note: It is always necessary to check the performance of the
pipette after in-house service or maintenance.

11.3. Sterilization of the pipette

The entire mLINE pipette can be

sterilized by steam autoclaving
at 121°C, (252°F), 1 atm for
20 minutes. Remove the tip
cone filter (if fitted, Chapter

8.3). The single channel
pipettes can be autoclaved
without special preparations.

Unscrew the lower part of
the multichannel pipettes by

holding the connecting collar and turning the lower part 360°
counterclockwise (Fig. 10). Put the pipette into the sterilization bag

and place it into the autoclave. After autoclaving the pipette must be
cooled down and left to dry overnight before use. Screw the lower
part of the multichannel pipet tes by holding the connecting collar and
turning the lower part 360° clockwise. Make sure that the lower part
is properly tightened. It is recommended to check the per formance of
the pipette after every autoclaving. It is also recommended to grease
the piston/seal of the pipette after every 10 autoclaving.

360°

Fig. 10.

Connecting
collar

12

12. TESTING THE PERFORMANCE AND RECALIBRATION

It is recommended to check the performance of your Biohit mLINE
pipettes regularly (e.g. every 3 months) and always after in-house
maintenance. However, the user should establish a regular testing
routine for their pipettes with regard to accuracy requirements of
the application, frequency of use, number of operators using the
pipette, nature of the liquid dispensed and the acceptable maxi­mum permissible errors established by the user. (ISO 8655-1.)

12.1. Testing the performance

Performance testing should take place in a draught-free room at
15 - 30°C, constant to +/- 0.5°C and humidity above 50%. The
pipette, tips and the test water should have stood in the test room
a suffi cient time (at least 2 hours) to reach equilibrium with the
room conditions. Use distilled or deionised water (grade 3, ISO

3696). Use an analytical balance with a readability of 0.01 mgs.
(ISO 8655-6.)

Weighing

1. Adjust the desired test volume V

s

.

2. Carefully fi t the tip onto the tip cone.

3. Fill the tip with test water and expel to waste fi ve times to reach

a humidity equilibrium in the dead air volume.

4. Replace the tip. Pre-wet the tip by fi lling it once with test water

and expel to waste.

5. Aspirate the test water, immersing the tip only 2-3 mm below

the surface of the water. Keep the pipette vertical.

6. Withdraw the pipette vertically and touch the tip against the

inside wall of the test water container.

7. Pipette the water into the weighing vessel, touching the tip

against the inside wall of the vessel just above the liquid surface

at an angle of 30° to 45°. Withdraw the pipette by drawing the

tip 8-10 mm along the inner wall of the weighing vessel.

8. Read the weight in mgs (mi).

9. Repeat the test cycle until 10 measurements have been

recorded.

10. Convert the recorded masses (mi) to volumes (Vi)
Vi = mi Z Z = correction factor (Table 1)

11. Calculate the mean volume (v ) delivered:
v = (∑V

i

)/10

12. For conformity evaluation calculate the systematic error es of

the measurement:
in μl: e

s

= v - V

s

V

s

= selected test volume

or in %: e

s

= 100 (v - V

s

)/V

s

13

13. For conformity evaluation calculate the random error of the

measurement:

as standard deviation s = ∑(V

i

- v )

2

n = number of

measurement (10)

n - 1

or as coeffi cient of variation CV = 100

s/v

14. Compare the systematic error (inaccuracy) and random error

(imprecision) with the values in the performance specifi cations

- (p. 49) or the specifi cations of your own laboratory.

If the results fall within the specifi cations, the pipette is ready

for use. Otherwise check both systematic and random errors

and, when necessary, proceed to the recalibration procedure

(Chapter 12.2).

Note: Systematic error (inaccuracy) is the difference between the
dispensed volume and the selected test volume. Random error
(imprecision) is the scatter of the dispensed volumes around the
mean of the dispensed volume. (ISO 8655-1.)

Note: Biohit specifi cations are achieved in strictly controlled condi-
tions (ISO 8655-6). The user should establish own specifi cations
based on the fi eld of use and the accuracy requirements placed on
the pipettor (ISO8655-1).

Table 1

Note: This method is based on ISO 8655.

Z-values (μl/mg):
Temp. (°C) Air Pressure (kPa)
95 100 101.3 105

20.0 1.0028 1.0028 1.0029 1.0029

20.5 1.0029 1.0029 1.0030 1.0030

21.0 1.0030 1.0031 1.0031 1.0031

21.5 1.0031 1.0032 1.0032 1.0032

22.0 1.0032 1.0033 1.0033 1.0033

22.5 1.0033 1.0034 1.0034 1.0034

23.0 1.0034 1.0035 1.0035 1.0036

23.5 1.0036 1.0036 1.0036 1.0037

14

12.2. Recalibration

The calibration of your mLINE pipette has been factory checked
by using forward pipetting technique and certifi ed at 22°C using
grade 3 distilled water according to ISO 3696. The calibration is
based on ISO 8655-6: Gravimetric test method for volumetric
instruments. The pipette's specifi cations are guaranteed only with
genuine Biohit tips. If you fi nd the pipette to be inaccurate after
performance testing, please follow the instructions below:

1. Remove the lid of the calibration nut, located at the backside

of the handle, with the aid of the calibration tool (Fig. 11).

2. Place the hexagonal head of the calibration tool into the hole

of the calibration nut.

3. Turn the adjustment lock counterclockwise to decrease and

clockwise to increase the volume.

4. Repeat testing the performance procedure (Chapter 12.1.).

Continue until the results are correct.

Note: Recalibration is recommended if reverse pipetting technique
is used.

Note: Biohit offers accredited calibration service. Please contact
your local Biohit representative for further information.

Fig. 11.

–

+

15

Symptom Possible cause Solution

Droplets left inside Unsuitable tip Use original tips
the tip

Leakage or pipetted Non-uniform wetting Attach new tip
volume too small of the plastic

Tip incorrectly Attach fi rmly
attached

Unsuitable tip Use original tips

Foreign particles Clean the tip cone,
between tip and attach new tip
tip cone

Tip cone holder Tighten the tip
incorrectly tightened cone holder

Pipette damaged Return to your
Biohit service
representative
for servicing

Pipette out of Incorrect operation Follow instructions
established
specifi cations Unsuitable tip Use original tips

Calibration altered Recalibrate

Operating button Liquid has penetrated Clean and grease
or moves tip cone and dried jammed the piston
erratically /seal and the tip
cone

Safe Cone fi lter has Change fi lter
been contaminated

Insuffi cient amount of Grease
grease on a piston accordingly
and seal

Tip ejector jammed Tip ejector collar has Remove and clean
or moves erratically been contaminated the ejector collar
and tip cone

13. TROUBLE SHOOTING

16

14. WARRANTY INFORMATION

The Biohit mLINE pipettes are covered by warranty for 3 years
against defects in materials and workmanship. Should your mLINE
pipette fail to function at any time, please contact your local Biohit
representative.

ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF
FAU LT I S F OU ND TO H AV E B EE N C AU SE D BY MA LTR E ATM EN T,
MISUSE, UNAUTHORIZED MAINTENANCE OF SERVICE OR
NEGLIGENCE OF REGULAR MAINTENANCE AND SERVICE,
ACCIDENTAL DAMAGE, INCORRECT STORAGE OR USE
OF THE PRODUCTS FOR OPERATIONS OUTSIDE THEIR
SPECIFIED LIMITATIONS, OUTSIDE THEIR SPECIFICATIONS,
CONTRARY TO THE INSTRUCTIONS GIVEN IN THIS MANUAL
OR WITH OTHER THAN THE MANUFACTURER’S ORIGINAL
TIPS.

Each Biohit

mLINE pipette is tested before shipping by the

manufacturer. The Biohit Quality Assurance Procedure guarantees
that the Biohit mLINE pipette you have purchased is ready for
use.

Each Biohit mLINE pipette is CE/IVD marked.

15. PERFORMANCE SPECIFICATIONS

The manufacturer's specifi cations (p. 81) are guaranteed only
when the manufacturer's original tips are used. The manufacturer's
specifi cations should be used as guidelines when establishing your
own performance specifi cations in accordance with ISO 8655.

Specifi cations

Biohit mLINE single-, 8- and 12-channel pipettes

Volume Test
Cat. No. Channels Range Volume

Inacc. (%) Impr. (%)

725010 1 0.1 - 3 μl 3 μl 1.30 % 0.80 %

1.5 μl 2.40 % 1.60 %

0.3 μl 10.0 % 6.00 %

725020 1 0.5 - 10 μl 10 μl 1.00 % 0.60 %
5 μl 1.50 % 1.00 %
1 μl 2.50 % 1.50 %

0.5 μl 5.00 % 4.00 %

725030 1 2 - 20 μl 20 μl 0.90 % 0.40 %
10 μl 1.20 % 1.00 %
2 μl 3.00 % 2.00 %

725050 1 10 - 100 μl 100 μl 0.80 % 0.15 %
50 μl 1.00 % 0.40 %
10 μl 2.00 % 1.00 %

725060 1 20 - 200 μl 200 μl 0.60 % 0.15 %
100 μl 0.80 % 0.30 %
20 μl 2.00 % 0.80 %

725070 1 100 - 1000 μl 1000 μl 0.60 % 0.20 %
500 μl 0.60 % 0.20 %
100μl 1.00 % 0.40 %

725080 1 500 - 5000 μl 5000 μl 0.50 % 0.20 %
2500 μl 0.60 % 0.30 %
500 μl 2.00 % 0.60 %

725090 1 1 - 10 ml 10 000 μl 0,6 % 0,2 %
5000 μl 1,2 % 0,3 %
1000 μl 3,0 % 0,6 %

725120 8 0.5 - 10 μl 10 μl 1.50 % 1.00 %
5 μl 2.50 % 2.50 %
1 μl 4.00 % 4.00 %

725130 8 5 - 100

μl 100 μl 0.70 % 0.25 %
50 μl 1.00 % 0.70 %
10 μl 3.00 % 1.50 %

725140 8 30 - 300 μl 300 μl 0.60 % 0.25 %
150 μl 1.00 % 0.50 %
30 μl 2.00 % 1.00 %

725220 12 0.5 - 10 μl 10 μl 1.50 % 1.00 %
5 μl 2.50 % 2.50 %
1 μl 4.00 % 4.00 %

725230 12 5 - 100 μl 100 μl 0.70 % 0.25 %
50 μl 1.00 % 0.70 %
10 μl 3.00 % 1.50 %

725240 12 30 - 300 μl 300 μl 0.60 % 0.25 %
150 μl 1.00 % 0.50 %
30 μl 2.00 % 1.00 %

CHINA

Biohit Biotech (Suzhou) Co Ltd.
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

FRANCE

Biohit SAS
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

GERMANY

Biohit Deutschland GmbH
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

JAPAN

Biohit Japan Co., Ltd.
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

RUSSIA

Biohit OOO.
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

U.K.

Biohit Ltd.
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

U.S.A.

Biohit Inc.
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

Biohit Oyj

Headquarters

Laippatie 1, 00880 Helsinki,
Finland
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]
www.biohit.com

mLINE

®

Instruction Manual

Bedienungsanleitung

Mode d’ emploi

Manual Usuario

Instruzioni d’impiego

Инструкция пользователя

400208.07 • 10/2010 • © Biohit Oyj

All information contained in this publication is current at the time
of publication and whilst the greatest care has been taken over its
compilation, Biohit is not responsible for any errors or omissions
and reserves the right to make changes without notice. All
trademarks are Biohit property unless otherwise stated. Patents
granted or pending

Alle in diesem Dokument veröffentlichten Daten entsprechen
den aktuellen Erkenntnissen und wurden mit größter Sorgfalt
zusammengetragen. Biohit ist nicht verantwortlich für Fehler oder
Auslassungen und behält sich das Recht vor, Änderungen ohne
Ankündigung vorzunehmen. Alle Marken sind Eigentum von Biohit,
soweit nicht anders angegeben. Patente wurden gewährt oder
sind schwebend.

Toutes les informations contenues dans cette publication sont à
jour au moment de la publication et ont été compilées avec le plus
grand soin, Biohit n’est pas responsable des éventuelles erreurs ou
omissions et se réserve le droit d’apporter des modifications sans
préavis. Toutes les marques sont la propriété Biohit sauf indication
contraire. Brevets délivrés ou en attente.

Toda la información contenida en este documento es actual al ser
publicada, y si bien se ha tomado la mayor atención al realizarlo,
Biohit no es responsable en caso de errores u omisiones, y se
reserva el derecho de aplicar cambios sin previo aviso. Todas las
marcas registradas son de propiedad de Biohit a menos de que no
se estipule lo contrario. Patentes concedidas o pendientes.

Tutte le informazioni contenute nella presente pubblicazione sono
aggiornate al momento della pubblicazione e pur essendo stata
usata la massima cura nella compilazione, Biohit non risponde
di eventuali errori o omissioni e si riserva il diritto di apportare
modifiche senza preavviso. Tutti i marchi sono dei proprietà di
Biohit se non diversamente specificato. Brevetti concessi o in
corso di registrazione.

Вся информация, которая содержится в данной инструкции,
является действительной на момент публикации и несмотря
на то, что сбор данной информации производился с особой
тщательностью, компания Biohit не несет ответственност и
за содержащиеся в ней ошибки и неточности, а также
оставляет за собой право вно сить в нее любые изменения без
предварительного уведомления. Все торговые марки являются
собственностью Biohit, если не указано иное. Все патенты
получены.

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