CoolCell® SV2
Instructions for Use
BCS-172
Controlled-rate cell freezing container
Quick Start
• The 12 chambers and 2ml serum vials should be dry to avoid tube sticking upon freezing.
• Make sure the core (black ring) is at room temperature and seated in the bottom of the central cavity.
• Place sample vials containing 1ml of cell suspension in each well. For reproducible freezing profiles, each
well should contain a filled vial; “blank” vials containing 1 ml of freezing medium should be placed in
empty chambers if freezing less than 12 vials of cell suspension.
• Check that the tubes slide in and out freely.
• Fully seat the lid on CoolCell SV2 container.
• Place the CoolCell SV2 container into a -80
o
C freezer or dry ice locker. Ensure that there is at least one
inch of free space clearance around the CoolCell SV2 module.
• Freeze for four hours before transferring samples to archive storage.
Transferring frozen samples to archive storage
• Prepare an insulated pan or container with a one inch (2.5cm) layer of pulverized or pellet dry ice.
• Remove CoolCell SV2 container from the freezer and gently remove the lid using a twisting and rocking
motion.
• Vial tops will be exposed once lid is removed and vials should be quickly extracted and placed onto the
dry ice.
Special Notes:
• Always use dry ice to transfer frozen vials containing cells to permanent storage to avoid temperature
rise and cell damage. Vial contents can rise from -80
o
C to over -50oC in less than one minute if exposed to
room temperature air.
• It is strongly recommended that all frozen cell cultures be checked for viability before the stock culture is
terminated.
Recycling CoolCell SV2 to room temperature
The CoolCell SV2 container is ready to freeze again as soon as the core (black ring) is at room temperature.
To rapidly recycle CoolCell SV2 container to room temperature, remove the center solid core ring by inverting
and tapping on a surface. CoolCell SV2 body and lid will return to room temperature in 10 to 15 minutes.
Check that all chambers are dry. Dry the core ring before re-inserting into the central chamber.
About CoolCell SV2
The CoolCell SV2 contianer, in combination with a -80
o
C freezer or dry ice locker, will provide the freezing
rate of -1
0
C per minute that is ideal for cryo-preservation of most cultured cell lines. CoolCell SV2 design
uses a combination of insulation foam, radial symmetry, and a heat transfer core to regulate heat loss rather
than using a large thermal mass (alcohol based freezing container). As a result, freezing profiles are extremely
consistent from one run to the next. Also, because of this low thermal mass, the CoolCell SV2 container will
not cause a rise in local freezer temperature and will protect nearby samples already stored in the freezer.
Low thermal mass also means the CoolCell SV2 container will rapidly return to room temperature for another
freezing cycle (see fast recycle instructions above).
CoolCell SV2 freezing performance
The CoolCell SV2 container will freeze 12 serum vials each
containing 1ml of cell suspension at -1
0
C per minute when
placed in a -80
o
C environment (mechanical freezer or dry
ice locker). The graph (left) displays a typical freezing
profile obtained using these conditions.
Troubleshooting
Care and cleaning
The CoolCell SV2 container is constructed of closed cell crosslinked polyethylene foam and a solid
thermoconductive core. The CoolCell SV2 container is compatible with prolonged cryogenic temperature
exposure. The foam may be cleaned by water and mild soap. Rinse and dry thoroughly. CoolCell SV2 is
resistant to alcohols and 10% bleach solutions. Do not autoclave. Maximum temperature exposure: 60
o
C.
Avoid prolonged exposure to UV light sources.
Problem Solution
Vials do not freely fit in the
chambers
CoolCell SV2 is designed to fit standard serum vial shaped vessels up
to 14.9mm in diameter and up to 37 mm in height. Check that flag style
labels, if used, will not bind and hinder insertion or removal.
Vials are stuck in CoolCell SV2
after freezing
It is likely moisture was in the vial chambers or on the sample vial prior
to freezing. Remove the core (black ring) and tap the CoolCell SV2 to
dislodge vials.
The lid does not fully seat Ensure that sample vials are properly seated. The maximum height of the
vial is 37mm.
12 E. Sir Francis Drake Blvd, Suite B, Larkspur CA, 94939 USA info@biocision.com www.biocision.com
CoolCell SV2 Instructions, May 2014
CoolCell SV2. ©2014. BioCision, LLC. All rights reserved. Patents pending. BioCision, Standardizing Samples, CoolCell designations are
trademarks owned by BioCision, LLC.
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