Bertin Technologies Cryolys Evolution User Manual

Document reference: CLYS-800-DU001-A

Original Instructions

Cryolys

®

is a registered trademark of Bertin Technologies.

© 2017 Bertin Technologies. All rights reserved

For research use only

Document reference: CLYS-800-DU002-A

Translated instructions from the French original version

Cryol ys® is a regi stered tra demark of Be rtin Techn ologies.

© 2017 Bertin Technologies.

All rights reserved

For Research purposes only

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Restricted User License:

By purchasi ng or u sing th e Produc t, the use r acc epts th e terms and c ondi tio ns of
this License in full.

The purchase or use of the Product confers on the User the non-transferable right
to use the Product for Research purposes only. The term Research denotes an y
scientific research programs conducted directly by the User for their own
purposes; the User renounces the right to use the Product for the purposes of
developing a tr eatment or diagn ostic method as well as any use of th e Product
for Commercial purposes.

The term "Commercial" includes in particular use of the Product or its
Components, (i) for manufacture, (ii) for the pr ovision of services, information or
data to a Thi rd Par ty, (e .g. pai d sample testi ng), (iii ) for r esale , whether with in the
context of Research or not, (iv) for quality control or providing quality assurance
for the manufacturer of a commercialized product.

The User is not authorized to modify, resell or transfer the Product or its
components.

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Legal Information:

Bertin Technologies and/or its subsidiaries deny any liability either explicit or
implicit and particularly but not exclusively for commercial or individual
warranties linked to this document.

Except where otherwise provided for by law, neither Bertin Technologies nor its
subsidiaries can be held liable, either in contractual or cri minal law, and in terms
of a guarantee or under any other regime that may be, for any damage in
whatever form which may occur particularly but not exclusively as a result of the
use of this document.

Neither Bertin Technologies, nor its employees, can be held liable for losses or
costs resulting from its use.

The information contained in this document is provided solely for use with this
Bertin Technologies product under the terms and conditions of the Restricted
User License. Bertin Technologies i s not liable for any use of thi s information when
applied to other products.

Bertin Technologies reserves the right to modify this document and make any
changes at any time without prior notice.

This liabili ty waiver cl ause is wri tten an d interpr eted accor ding to Fr ench law. Any
legal action relating to the content of this cl ause is the exclusi ve jurisdiction of the
Courts of Law in Paris, France.

© 2017 by Bertin Technologies.
This document is protected and contains information which is the property of

Bertin Instruments. The Bertin Instruments, Precellys

®

Evolution and Cryolys

®

Evolution logos are registered trademarks of Bertin Technologies.

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Table of Contents

............................................................................................................................................ 1

1 Introduction.................................................................................................................. 5

1.1 Safety instructions and recommendations ................................................... 5

1.2 Warranty .............................................................................................................. 9

2 Description of the Cryolys® Evolution .................................................................... 10

2.1 Description of the equipment ....................................................................... 10

2.2 Specifications ................................................................................................... 11

2.3 Generating cold air ......................................................................................... 12

3 Installing the Cryolys® Evolution .............................................................................. 14

3.1 Unpacking and installation ............................................................................ 14

3.2 Packaging and transport ............................................................................... 19

3.3 Storage .............................................................................................................. 20

4 Use ............................................................................................................................... 21

4.1 Description of user interface.......................................................................... 21

4.2 Starting tempe rature-controlled homogenization .................................... 22

5 Cleaning and decontamination............................................................................ 26

5.1 Recommendations .......................................................................................... 26

5.2 Decontamination protocol ............................................................................ 26

6 Support and documentation ................................................................................. 27

6.1 Frequently asked questi ons ........................................................................... 27

6.2 Troubleshooting ................................................................................................ 28

6.3 Support .............................................................................................................. 29

6.4 Disposal .............................................................................................................. 29

7 Appendix: Example of CE Declaratio n ................................................................. 30

8 Appendix: Electrical diagram ................................................................................. 31

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1 INTRODUCTION

Thank you for purchasin g a Cryolys® Evolution by B ertin Instruments.
The Cryolys® Evolution is a cooli ng system specifically designed fo r the Precellys®

Evolution. Based on the use of cooled atmospheric air through the sublimation of
dry ice, it provides optimal protection for temperature-sensitive molecules such
as proteins, small RN As or even parti cular chemical c ompounds by mai ntai nin g a
temperature between 0 and 10°C. The Cryolys

®

Evolution is the solution for
preserving the molecules in your samples and therefore for obtaining the best
possible results.

This user manual inclu des all the infor mation you need to use and maintain your
Cryolys

®

Evolution. The technical specifications of this product, along with all the
information contained in this manual may be modified at any time with out prior
notice.

1.1 Safety instructions and recommendations

Read this user manual carefully before using your Cr yol ys® Evolution.
If you are in any doubt about the safety of the device, please contact us by

email at thi s address: [e-mail] or contact your local
distributor, whose address is listed on our website: Bertin Instruments.

Bertin Technologies cannot be held liable for any damage or injuries caused by
using this device in a manner other than that described in this manual.

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Reference labels are attached to the device's components. Users must comply
with the info rmation printed th ereon.

1.1.1 Risk of electric shock

As the Cryolys

®

Evolution is connected electrically to the rear panel of the

Precellys

®

Evolution, usage precautions and recommendations against electric

shocks are as described in the Precellys

®

Evolution User Manual (02520-800-

DU003-B).

1.1.2 Biological risks

Always wear the necessary personal protection equipment (PPE) when cleaning
any parts soiled by biological samples, for example inside the Precellys

®

lid.

Note : Refer to the Prec ellys

®

Evolution manual (02520-800-DU003-B ) as well as to

the procedures cur rently in place in your laboratory

Eye and face protection

Hand and skin protection

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1.1.3 Risks linked to dry ice

Dry ice (cardice) is made from an accumulation of carbon dioxide in a solid
state with a temperature of -80°C.

It sublimates in air into CO2, a colorless and odorless gas. The principal risks linked
to the handling of this product are asphyxia and burns. In order to protect
yourself against projectiles and the risk of asphyxia, the user must wear protective
gear for the eyes, face and skin. Store and handle dry ice in a ventilated area.

CAUTION! Ensure you are in possession of the safety data sheet covering the risks
and precautions linked to the use of dry ice (handling using protective gloves,
transport and storage under particular conditions, etc.).

1.1.4 Mechanical risks

The lid is liable to self-closure. We recommend keeping it in the open position
with your hand during all operations requiring it to be opened. If not, we
recommend closing it.

When opening the lid, please be aware of the risk of pinching between the
hinge and the rear of the Precellys

®

Evolution.

Risk of burns: Dry ice at -80°C

Risk of asphyxia

RISK OF

PINCHING

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1.1.5 Special Safety Symbol

Comply with the safety instruc tions associated with this symbol.

1.1.6 Recommendations

Any use of this device other than that described in this manual can alter its
external protection and damage its internal components.

We recommend that you:

- Do not install unauthorize d components or acce ssories; th is c an alte r the
protectio n afforded by the device and void its warranty.

- Do not drop the device: the Cryolys

®

Evolution module must always sit

atop the Precellys

®

Evolution or on a horizontal surface, or its

comp o n e n t s may bec o me damaged .

- Dot not handle the device if it is fully or partially dismounted or if it is
damaged.

- Do not invert the connections as this could irreparably damage the
connectors.

- Do not plug the Cryolys

®

Evolution in to a power supply other than the

one supplied by Bertin Instruments.

- Do not obstruct the fan.

- ONLY use sticks of dry ice (do not use nitrogen or H

2

O ice).

- Do not fill the dry ice container beyond its limit (see "4. How to Use")

- Do not ship the device if the container contains dry ice.

- Do not ship the device in anything other than the original packaging.

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1.2 Warranty

The manufacturer's warranty is limited to one (1) year. This war ranty is valid from
the date the equipment is registered on our website: (www.bertin­instruments.com). This covers failure or malfunc tion of any of the components in
the device where it has been used in compliance with the recommendations
contained in this manual.

The warranty does not apply in the following cases:

- Use or maintenance which does not comply with the instructions contained in
this manual.

- Opening, repair or modification of the device by unauthorized persons.

- Alteration or removal of the label on which the device's serial number is printed.

Manufacturer:
Bertin Technologies
Parc d’acti vité du Pas du Lac
10bis Avenue Ampère
78180 MONTIGNY-LEBRETONNEUX
FRANCE

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2 DESCRIPTION OF THE CRYOLYS® EVOLUTION

The Cryolys® Evolution is a device designed to keep biological samples in the
Precellys

®

Evolution at a temperature between 0°C and 10°C to avoid any

degradation l inked to a temperature increase during homogenization.
The Cryolys® Evolution allows you to:

- Keep the sample temperature below 10°C throughout the
homogenization process.

- Control the temperature from the Precellys

®

Evolution’s screen.

- Protect temperature-sensiti ve molecules from degradation.

2.1 Description of the equipment

5

6 4 3 1 2 9 7

8

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1

Main seal: ensures a good seal between the cover and the module.

2

Diffuser Plate: this is attached to the cover and directs air over the dry ice.

3

Cooling module: ensures uniform diffusion of cooled air inside the Precellys®

Evolution.

4

Module cover: Encloses the cooling module.

5

Locking levers: allow removal or locking of the coolin g module from/to the

Precellys® lid.

6

Precellys® lid: its fixed base receives the cooling module.

7

Fan: propels atmospheric air through the module. The four-pin connector

must be connected to the back of the Precellys® Evolution.

8

Temperature sensor: measures the temperature inside the Precellys®

Evolution an d detec ts whethe r the module is attac hed to th e Precellys® lid.
The three-pin connector must be connected to the back of the Pr ecellys

®

Evolution.

9

Hinge reinforcement: reinforces the hinge of the Precellys® Evolution in order

to support the Cryolys® Evolution.

2.2 Specifications

Technical specifications

Supply voltage

110 - 230V

Power consumption

<1 kVA

Physical specifications

Width

330 mm

Depth

336 mm

Height

111 mm

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1: The atmospheric air propelled by the fan is cooled as it
travels over the dry ice

2: The cold air is injected into the tube container via the
Precellys® lid

1

2

Figure 1: cooling p rinciple

Weight (empty module)

1.1 kg

Operating temperature

15-30°C

Humidity

15-85% RH

Altitude

< 2,000 m

Operating specifications

Maximum air flow

168 m3/hour

Dry ice reservoi r

1.8 kg

Dry ice consumption

Approx. 1.5 kg/30 mins

Min imum d ry i ce volume

0.5 kg

Noise level

< 60dB

Temperature control

Between 0°C and 10 °C (1°C increments)

2.3 Generating cold air

Cold air is pro duced and maintain ed at the temperature set by the user.

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Temperature during homogenization without Cryolys® Evolution

Temperature during homogenization with Cryolys® Evolution
Precellys

®

Evolution homogenization cycles

Figure 2: Temperature inside th e tu bes during homogenization with and

without Cryoly s® Evolution

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3 INSTALLING THE CRYOLYS® EVOLUTION

3.1 Unpacking and installation

It is important to check the delivered package carefully and inform your carrier
of any damage.

3.1.1 Package contents

The package contains the following items:

- One Cryolys® Evolution (module + specific Precellys® lid)

- Two mountin g s crews attached to the Pre cellys® lid

- Hinge reinforcement for the Precellys

®

Evolution

- Cable tie

- USB stick containing Cryolys

®

Evolution control software

- User manual

- CE compliance decl aration.

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3.1.2 Unpacking the package

1. Open the cardboard

box.

2. Pull out the inner box

using the central handle.

3. Open the inner box

and pull out the top foam
protector.

4. Check that all

components are
present.

5. Separate the Cryolys®

Evolution from its bottom
protector, and place it on a
flat surface.

6. Lift the hinge

reinforcement out of
the bottom protector

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3.1.3 Installing the Cryolys® Evolution

Note: Make sure the Precellys® Evolution is disconnected from the power supply before
installation.

Required tool: No 5 Allen (hex) key

1. Unscrew the 2 screws

on the cover of the
Precellys

®

Evolution

2. Detach the hinge and

remove the original
cover

3. First remove the

cooling module and then
place the lid on top of
the Precellys

®

Evolution

CAUTION - IMPORTANT

Do not hold the Cryolys® Evolution by its cover

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4. Insert the hinge

reinforcement into the
hinge screw holes

5. Gently tighten the 2

screws supplied with the
cover

6. Connect both fan and

temperature sensor
connectors to their
respective connections
at the back of the
Precellys

®

Evolution.

7. Attach the fan and board interface cables to the hinge reinforcement using the

cable tie provided.

3.1.4 Updating the software

In order to optimize the use of your instrument, we recommend always having
the latest available software version. You can check the installed software
version via the instrument's "settings" menu.

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Registering your product will ensure you are informed when new software
versions are made available for the Precellys

®

Evoluti on. Th is can be do ne via th e

Bertin Instruments website (www.bertin-instruments.com).

1. Insert the USB stick

containing the latest
version of the software.

2. Turn the Precellys®

Evolution on and select
"Data manager (USB)"
from the Menu.

3. Select "Update

software version".

Figure 3: Precellys® Evolution "settings" menu

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4. Press OK and wait 60 seconds.

5. The Precellys® Evolution will restart

automatically.

3.2 Packaging and transport

Before transporting the device, you must:

1. Check that there is absolutely no dry ice inside the module

2. Ensure there is no hu midi ty or, if necessary, dry any humid areas

If you have to transport or ship the Cryolys

®

Evolution, arrange and pack it by

following these steps:

1. Separate the module

from the Precellys® lid.

2. Disconnect both fan

and board interface
connectors from their
respective connections
at the back of the
Precellys

®

Evolution.

3. Unscrew the 2 screws

on the cover of the
Precellys

®

Evolution.

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4. Detach the hinge

reinforcement from the
hinge on the Precellys

®

Evolution.

5. Lift the lid off the

Precellys® Evolution.

6. Replace the module

on top of the Precellys®
lid and place the entire
unit inside the foam
protector and on the
inner box

7. Place the top foam

protector over the Cryolys®
Evolution

8. Close the inner box

9. Place inside the main

cardboard box using
the handle

3.3 Storage

The device must be stor ed i n a dr y loc ati on at a tempe r atur e betwe en +0°C an d
+50°C on a horizontal surface.

The device may be stored after checking the following points:

1. The module is empty

2. The device is dry, clean and decontami nated

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4 USE

4.1 Description of the user interface

4.1.1 Main interface

Figure 4: Precellys® Evoluti on main interface sc r e en

1

Mixer protocol settings interface: see Precellys® Evolution user manual

(02520-800-DU003-B).

2

Cooling protocol settings’ interface: allows you to set the various

parameters of the Cryolys® Evolution.

Note: the temperature display disappears and the Cryolys® Evolution interface is
grayed-out and deactivated.

3

Current temperature: indicates the temperature under the Precellys® lid.

4

Cryolys® Evolution module activation field (ON/OFF) : al lows you to ac ti vate

or deactivate th e Cryolys® Evolution

6

5

3 4 2

1

1

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5

Operating temperature selection field: allows you to set the temperature

between 0 and 10°C

6

Operating mode selection field: allows you to choose manual or automatic

mode

Note: for information about navigating the Precell ys® Evolution from the screen interface,
please refer to the Precellys

®

Evolution user manual (02520-800-DU003-B).

4.2 Starting temperature-controlled homogenization

4.2.1 Installing your Cryolys® Evolution

Use of the Cryolys® Evolution requires dry ice sticks.

Note: Wear personal protection equipment (PPE) as described in paragraphs 1.1.2 and

1.1.3.

CAUTION! Do not inhale dry ice vapors: risk of asphyxia.
Ensure the dry ice source container is closed after filling the Cryolys® Evolution.
Use the device in a ventilated area.

1

Fill the cooling module with dry ice

Note: Fill the cooling module up to the level of the interior fins to optimize the
production of cool air.

2

Close the cooling module with the cover

3

Clip the cooling module to the Precellys® lid

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4.2.2 Prepare the samples

1

Place the samples into the Precellys tubes and add the required buffer if

necessary

Note: Bertin Instruments recommends the use of the Precellys® Lysing Kits.

2

Close the tubes and arrange them ad hoc in the tube holder

Note: Bertin Instruments guarantees the compatibility of all Precellys® consumables
and accessories with the Cryolys

®

Evolution

4.2.3 Homogenizing the samples

Select a saved program or use the "work" function (See the Precellys® Evolution
user manual 02520-800-DU003-B)

Note: Saved protocol s must include Cryolys® Evolution parameters. The Cryolys® Evolution
module's activation fi eld must be set to ON.

The Cryolys® Evolution allows two operating modes, one automatic and one
manual.

CAUTION! Do not open the Precellys® Evolution lid or the cooling module cover
until the cycle completes.

Figure 5: diagram showing automatic and manual modes

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1

Program the homogenization parameters via the dedicated interface:

volume, speed, number & duration of cycle and pause

2

Program cooling parameters via the dedicated interface: ON/OFF,

temperature and mode

Note: You can use a saved protocol if the Cryolys® Evolution module activation field is set
to ON.

Automatic mode

3a

Place the tubes and tube holder (fitted with the plate) into the Precellys®

Evolution (refer to the Precellys® Evolution user manual 02520-800-DU003-B)

4a

Close the Precellys® lid

5a

Press the START button on the Precellys® Evolution

Note: When the chosen temperature is reached, the following
message appears: "Temp. is reached." Protocol starting". The
homogenization cycle star ts.

6a

At the end of the cycle, open the Precellys® lid and retrieve the tubes and

the tube holder

7a

Insert new samples and close the Precellys® lid

Note: Ensure that the volume of dry ice is sufficient. If no more homogenization is
required, empty the cooling module.

Manual mode

3m

Close the Precellys® lid

4m

Press the START button on the Precellys® Evolution

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5m

When the chosen temperature is reached, the following message

appears: "Temp. is reached." Open the Precellys® lid

6m

Place the tubes and the tube holder (fitted with the plate) into the

Precellys® Evolution (refer to the Precellys® Evolution user manual 02520­800-DU003-B)

7m

Close the Precellys® lid

8m

Press the START button on the Precellys® Evolution

9m

At the end of the cycle, open the Precellys® lid and retrieve the tubes

and the tube holder

10m

Insert new samples and close the Precellys® lid

Note: Ensure that the volume of dry ice is sufficient. If no more homogenization is
required, empty the cooling module.

4.2.4 End of cycle

If there are no more tests to carry out:

1. Empty the dry ice from the Cryolys

®

Evolution into a suitable container.

2. Clean the Cryolys

®

Evolution using a wipe (reservoir, reservoir air outlet,

interior of di ffuser module, etc .) .

3. Turn the Cryolys

®

Evolution switch to OFF.

CAUTION! Avoid all direct contact between your skin and cold parts of the
Precellys

®

Evolution and Cryolys® Evolution.

Do not heat up the dry ice using a hair dryer or similar in order to accelerate
evaporation.

Handle only with suitable gloves whenever the module contains dry ice.
If using the Precellys® Evolution without cooling, ensure that the Cryolys® Evolution

is fitted to the diffusion cover to avoid any projectiles in the event of a leak from a
tube.

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5 CLEANING AND DECONTAMINATION

5.1 Recommendations

For safety reasons, and to avoid damaging the instrument, follow these
instructions:

- Do not spray anything directly onto the device, particularly on the
electrical connectors.

- Clean the instrument when it is unplugged.

- Do not use a sponge-scourer to avoid damage to the device.

- Do not use an airborne decontamination process.

- Do not use caustic soda crystals or acetone which will irreparably

damage the equipment .

- Immediately remove any surplus liquids using a dr y cloth .

The exterior of the device can be cleaned using a sponge or cloth which has
been dampened with water or household alcohol.

5.2 Decontamination protocol

In the event of splashing, any parts that might have been contaminated by
infectious agents must be decontaminated using a suitable disinfectant.

The user alone is responsible for the choice of decontamination procedure.
Any parts that might have been contaminated may, for example, be cleaned

using a wipe dipped in bleach diluted to 6° Cl.

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6 SUPPORT AND DOCUMENTATION

6.1 Frequently asked questions

Question

Answer

Can the Cryolys® Evolution fall off when
I open the cover of the Precellys

®

Evolution?

We recommend separating the cooling
module containing dry ice from the
cover when operating the cover

Can I use any type of ice (ice cubes,
blue ice, ice gel, etc .) instead of dry
ice?

The Cryolys

®

Evolution must absolutely

be used with dry ice sticks only

Is it possible to save a protocol's cooling
parameters?

Just as for Precellys® Evolution protocols,

you can save a protocol using the
Cryolys® Evolution. Homogenization and
cooling parameters will be saved
simultaneously.

What is the maximum/minimum
quantity of dry ice that I can plac e
inside the Cryolys

®

Evolution?

We recommend a minimum of 0.5 kg of

dry ice in the module which appears as
a uniform layer across the bottom of the
module.

The maximum is defined by your ability
to close the module cover, or around

1.5 kg of dry ice.

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6.2 Troubleshooting

Problem

encountered

Possible cause

Action(s) to be performed

Message
appears: "No
Cryolys

®

Evolution
has been
detected"

Module is badly

positioned

Reposition the module on the

Precellys® lid.

Bad connection

1. Check that the interface

board connector is
plugged in

2. Check the state of the
interface board, its cable
and connector.

Message "Temp.
not reached" or
"Te mp. not
maintained
during protocol"
appears

Not enough dry ice

Check that the dry ice covers

at least the center of the
reservoir.

The cooling system is not
receiving any air

1. Check that the fan

connector is plugged in

2. Check the state of th e fan,
its cable and connector.

Diffuser holes are bloc ked

Wait for the i nterior of the

module to defrost.

Cut the power

Disconnect the Precellys

®

Evolution from the power
supply

If any of these faults persist, contact technical support (see 6.3 Support).

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6.3 Support

If, despi te the in formati on c on tai ne d in thi s manu al, yo u ar e sti ll u na ble to re solve
your problem, please contact your distributor.

For the latest information about our services and support, please go to our
website http://www.bertin-instruments.com.

You can also contact the Bertin Instruments team vi a email at:

[e-mail]

.

6.4 Disposal

This equipment is subject to selective collection in accordance with the
European directive on waste electrical and electronic equipment (WEEE)
2012/19/EU.

Bertin Technologies is responsible for treatment of WEEE for
products sold in France (via RECYLUM). For other countries, the
importer i s responsibl e for treatmen t and this was te treatmen t is
dependent on current regulati o ns.

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7 APPENDIX: EXAMPLE OF CE DECL A R ATION

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8 APPENDIX: ELECTRIC AL DIAGRAM

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