UV Transilluminator
Model: E3000
Operation Manual
Version 10.16
99 Washington Street
Melrose, MA 02176
Phone 781-665-1400
Toll Free 1-800-517-8431
Visit us at www.TestEquipmentDepot.com
I. Overview
The Accuris E3000 UV Transilluminator is a laboratory instrument designed to
produce a uniform source of UV light for the excitation of fluorescent dyes
such as Ethidium Bromide. The E3000 is ideally suited for observation,
analysis and photo imaging of stained electrophoresis gels that are positioned
on the glass viewing surface.
The midrange wavelength of the E3000 Transilluminator is 302nm. Other
manufacturers of transilluminators may refer to the same wavelength range as
300nm, or 312nm, but the spectral range is the same.
II. Warnings
UV Transilluminators are powerful sources of UV radiation, which
is hazardous to the skin and eyes.
Always make sure the protective UV blocking cover is positioned to
prevent UV exposure to the user, and wear protective clothing and
protective glasses to prevent UV exposure to the skin and eyes.
Although the UV blocking cover will effectively block UV radiation,
it is always recommended to wear protective UV blocking glasses.
Power / High / Low Switch
UV protective cover
Cooling Fan
Cover hinges
Use caution when cutting gels on the glass surface. Although
this surface is scratch resistant, use minimal pressure when
working with metal blades or tools.
II. Installation
Install the E3000 Transilluminator on a level and stable surface such as
laboratory counter, close to an available electrical outlet. The instrument
should be oriented with the power switch and hinges facing the user.
Leave sufficient space around the instrument to allow proper airflow, and
take care not to block the cooling fan on the right, rear side of the housing.
Make sure that the front power switch is set to the middle position (OFF),
and then connect the included electrical cord to the back of the instrument
and to an appropriately rated electrical outlet.
Note: The E3000 is available in 2 versions, for input voltage of 110V or 230V.
Check the rating plate on the rear housing to confirm use of the correct input
voltage.
III. Operation
1. Place a gel or sample on the viewing surface of the transilluminator.
2. Position the UV blocking cover to prevent exposure.
3. Turn on power to the UV light by pressing the front switch, choosing
either High or Low Intensity.
4. Note: Low intensity (50%) is used for preparation, positioning or
longer term exposure of the sample and will prevent excessive UV
damage.
5. High intensity (100%) will maximize excitation of the fluorescing dye,
and can be used for photography or visualization of small quantities
of sample.
IV. Imaging using the Accuris SmartDoc™
To image gels on the E3000 Transilluminator, it
is recommended to use an imaging enclosure.
V. Specifications
Light Source
UV, 8W bulbs
Transmission wavelength
302nm
Gel Viewing Surface
160x190mm
Dimensions
265 x 325 x 140mm
Input voltage
110V±10% or 230V±10%
Frequency
50/60Hz
VI. Cleaning and Care
The glass surface should be cleaned regularly with a cloth dampened with
water, a mild soap solution, or ethanol.
In order to extend the working life of light tubes and UV glass filter, power
off the instrument when not in use.
For service, contact Benchmark Scientific’s Service Department at 908-7695555
using a smart phone camera, is designed to fit
the viewing surface of the E3000. Filters are
The Accuris SmartDoc™ enclosure, for photos
available for blocking the UV wavelength to
allow image capture with minimal background
fluorescence.
Visit us at www.TestEquipmentDepot.com
99 Washington Street
Melrose, MA 02176
Phone 781-665-1400
Toll Free 1-800-517-8431
Loading...