Benchmark H5000-H, H5000-HC Operation Manual

MultiTherm Shaker

Operations Manual

Item: H5000-H and H5000-HC

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Foreword

Thank you for purchasing a MultiTherm™ Shaker. This operations manual

contains instructions for the care of this Instrument. In order to use the

instrument properly, please read this manual carefully before operation.

Please keep it available for future use.

Prior to the initial operation:

Please check the Instrument and the accessories against the packing list

when you first open the shipping carton. If you find there is damage, a

discrepancy, or missing items, please contact the supplier immediately.

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Safety Warnings and Guidelines

1. Important operation information:

Please read this manual carefully prior to any attempt to operate the Instrument.

Read the manual prior to power up of the instrument. Read the

guidelines and directions below and be familiar with all safety

warnin

2. Security:

The operation, maintenance and repair of the Instrument must comply with the

basic guidelines and warnings stated below. Failure to comply may jeopardize the

instrument life, warranty coverage and user safety.

s and guidelines.

Use this product indoors. Do not operate in a damp or wet

environment.

Do not attempt to disassemble or repair the Instrument by

yourself. Disassembly will void the warranty. If there is a

malfunction contact your supplier for repair service.

The three pin plug supplied with the instrument is a safety

device that should be matched with a suitable grounded outlet.

Do not attempt to defeat the three pin plug or its grounded

function.

During normal operation, the temperature of metal block will

become very hot. Do not touch the block during or within 30

minutes of operation.

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Close all test tube lids before inserting into the block. Do not

allow liquids to spill into the block or any part of the instrument.

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Before connectivity to a power source, check that the voltage

listed on the serial number label (on the bottom or back side of

the unit) to ensure that it matches the voltage available in your

area.

Be sure to install the Instrument in a cool, dry location away

from any direct sunlight or strong light sources. Additionally

the instrument should be kept away from corrosive gas,

magnetic fields and dusty environments. Keep the instrument

and the air vents at least 4 inches from walls, other instruments

or any obstructions.

The Main power switch is on the rear of the device,

push “I” to power on the instrument, and push “O” to

power off.

Shut the power off when you finish your work. Unplug the

machine if it will not be operated for a long time period and cover

it with a cloth or plastic paper for protection from dust particles

Immediately unplug the instrument from the outlet and

contact the vendor in the event of:

 Fire or smoke.
 Liquid has gotten into the Instrument;
 Abnormal operation: such as abnormal sound or smell.
 Instrument has been dropped or outer case is damaged.
 The function has visibly changed.

3. Maintenance of the Instrument

The block wells should be cleaned by a cloth dampened with alcohol to ensure

good heat transmission between the block and the test tube. If there are marks, dirt

or stains on the instrument exterior, clean them with a cloth dampened with water

or a mild detergent solution.

Always shut the power off when cleaning the Instrument.

When cleaning the block wells, do not apply the cleaning liquid

directly to the block, use a dampened cloth. Do not use strong or

corrosive cleaning agents.

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Contents

Chapter 1: Introduction 5

Chapter 2: Specifications 5

1. Normal operating conditions 5

2. Technical data 6

Chapter 3: Preparations 8

1. Physical Description 8

2. Keyboard and Display panel 9

3. Key Functions 10

Chapter 4: Operation Guide 11

1. Single Temperature, Speed and Time setting 11

2. Sequence Link Setting 12

3. Disabling the Temperature Speed or Time 12

4. Pulse 13

5. Temperature Calibration 13

6. Changing the Block 16

Chapter 5: Failure analysis and troubleshooting 17

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H5000 Series MultiTherm Shakers

Chapter 1: Introduction

The MultiTherm shaker is an ideal instrument for intensive mixing of samples under

temperature regulated conditions.

Mixing and temperature control modes can be used both simultaneously and

independently i.e, the device can work as shaker and/or as an incubator. The base

instrument can be used with a variety of different blocks. The instrument is applicable

for DNA analysis, extraction of lipids and other cell components, DNA library creation,
PCR amplification, pre-denaturation in electrophoresis, serum solidification etc.

Features of this product as follows:

1. Because it can be used with various optional mixing blocks, the instrument can

adapt to different tubes and plates to accommodate many experimental needs.

Customized blocks are available to suit your specific demands.

2. LCD display

3. Simultaneous display of set and actual time, temperature and speed

4. Over-heat protection system ensures safety & reliability

5. Temperature can be calibrated to meet the user's needs

6. End-of-run signal alerts the user of cycle completion

Chapter 2: Specifications

1. Normal operating conditions

Ambient temperature: 5C ～30C / Relative humidity: ≤70%

Note: Do not attempt to operate the instrument at temperatures or humidity levels

outside of this range.

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H5000 Series MultiTherm Shakers

2. Technical data, H5000-H

Model

H5000-H

Parameter

Mixing rate* 200~1500 rpm

Orbit 2mm

Temperature setting range 5°C to 100°C

Temperature control range* Ambient +5°C to 100°C

Timing range 1min to 99h59min

Accuracy of the temperature ≤0.5°C

Heating time

≤15min （from 20°C to 100°C）

Cooling time N/A

A-BLOCK: 96 well Plate or 96 x 0.2ml;

B-BLOCK: 0.5ml x 54 C-BLOCK: 1.5ml x 35

Block Configuration

D-BLOCK: 2.0ml x 35 E-BLOCK: 15ml x 12

F-BLOCK: 12mm x 24

H-BLOCK: 0.5ml x 20 & 0.5ml x15

AC100-120～ 2.5A

G-BLOCK: 50ml x 6

AC200-240

～ 1.5A

Power Supply

50-60Hz

50-60Hz

Fuse 250V 4.0A Ф5×20mm 250V 3.0A Ф5×20mm

Dimension (mm) 300(D)×2 12(W)×210(H)

Net weight (kg) 8.5

*Specification is valid when used with “standard” blocks only. Larger blocks (Blocks E, F and G)
have different speed and temperature specifications.

6

H5000 Series MultiTherm Shakers

Technical data, H5000-HC

Model

H5000-HC

Parameter

Mixing rate* 200~1500 rpm

Orbit 2mm

Temperature setting range 0° to 100°C

0° to 100° at Room temp. ≤20°C

Temperature control range*

4° to 100° at Room temp.≤25°C

10° to 100° at Room temp.≤30°C

Timing range 1min ~ 99h59min

Accuracy of the temperature ≤0.5°C

Heating time

≤15min （from 20°C to 100°C）

Cooling time ≤30min from room temp. to -20°C below room temp.

A-BLOCK: 96 well Plate or 96 x 0.2ml;

B-BLOCK: 0.5ml x 54 C-BLOCK: 1.5ml x 35

Block Configuration

D-BLOCK: 2.0ml x 35 E-BLOCK: 15ml x 12

F-BLOCK: 12mm x 24

H-BLOCK: 0.5ml x 20 & 0.5ml x15

AC100-120～ 2.5A

G-BLOCK: 50ml x 6

AC200-240

～ 1.5A

Power Supply

50-60Hz

50-60Hz

Fuse 250V 4.0A Ф5×20mm 250V 3.0A Ф5×20mm

Dimension (mm) 300(D)×2 12(W)×210(H)

Net weight (kg) 8.5

*Specification is valid when used with “standard” blocks only. Larger blocks (Blocks E, F and G)
have different speed and temperature specifications.

7

H5000 Series MultiTherm Shakers

Chapter 3: Preparations

This chapter provides a physical description, location of controls, function of

individual keys, as well as some preparations before the initial power-on. You

should be familiar with this chapter before the Thermo Shaker is first operated.

1. Physical Description

Standard Block

Keyboard

Power Switch

Power connector

Fuse

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H5000 Series MultiTherm Shakers

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2. Keyboard and Display panel

Display Panel：

Current program

P1100.0120010:30

P1100.0 120015:00

Selected pro

Current temperature

Selected temperature

ram

Current speed

Selected speed

Remaining time

Selected time

─ 9 ─

H5000 Series MultiTherm Shakers

y

3. Key Functions

Prog. 

Temp. 

Speed 

Time

Seq. Link

Pulse

Start/Stop

Program Selection Keys: Use the up and down keys to

choose the desired program (P1, P2, P3, P4 or P5).

Temperature Selection Keys: Press“” or “” to select the

desired temperature in increments of 1°C. (To quickly scroll to
the desired temperature, press and hold the“” or “” keys.)

Speed Selection Keys: Press “” or “” to select the desired

mixing speed in increments of 10rpm. (To quickly scroll to the
desired mixing speed, press and hold the“” or “” keys.)

Time Selection Keys: Press “” or “” to select the desired

run time. (To quickly scroll to the desired time, press and hold
the“” or “” keys.)

Sequence Link Key. Press the “Seq. Link” key to run

consecutive programs. Choose from P1 to P2, P1 to P3, P1 to

P4, or P1 to P5 to run up to five consecutive programs.

(The Sequence link function is only available on the H5000-HC.)

The device mixes at the speed shown on the display for as long

as the Pulse key is held down. The time is counted in seconds

until 999 Seconds has expired.

Start/Stop Key. press this key to start or stop the program. Press

to start. Press and hold to stop.

briefl

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H5000 Series MultiTherm Shakers

Chapter 4: Operation Guide

1. Single temperature, speed and time setting

a) Following power up, the unit beeps once and

the LCD will display “System Testing”. After

approx. 5 seconds, the current and selected

settings appear on the display and the

System‐Testing

......

instrument is ready for use.

b) The current block temperature is displayed

on the LCD, for example, 30.0 along with the current

mixing speed, 0 rpm and the current time, 00:00. The

set or selected temperature shows 37.0, which is the

initial preset temperature. The initial preset mixing

speed and time are also displayed at 1000rpm and

10:00 respectively.

c) Five programs are available for storage. By

pressing the “” or “” Prog. keys， choose the

desired program, P1, P2, P3, P4 or P5.

By pressing the “” or “” Temp. keys，the value of

the selected temperature is adjusted in increments of

0.1°C on the display window.

Prog 

P130.0000:00
P137.0100010:00

Temp 

P130.0000:00
P140.0100010:00

By pressing the “” or “” Speed keys，the value of

the selected mixing speed is adjusted in increments

of 10 rpm.

By pressing the “” or “” time keys，the value of

the selected run time is adjusted in increments of 1

min.

Speed 

P130.0000:00
P140.0120010:00

Time 

P130.0000:00
P140.012002:00

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H5000 Series MultiTherm Shakers

NOTE: Press and hold the “” or “” adjustment

keys to quickly scroll through the available

temperature, speed and time range

Press the

2.

Sequence Link Program Setting (Available on H5000-HC only)

Star/Stop Key to begin the cycle

a) By Pressing the “Seq. Link” Key, the user can connect up to five programs to run

consecutively (P1-P2, P1-P2-P3, P1-P2-P3-P4, P1-P2-P3-P4-P5).

b) Once the Seq. Link button is pressed, the display

shows the starting program “P1” and the default

ending program, “P2”. To adjust the ending program,

press the “” or “” Prog. Keys. Once the ending

program has been selected, press the Seq. Link key

to finalize.

PROGtovalidate
Star:P1End:P2

P130.0000:00

c) To begin the first program, press the “start/stop”key.

The first program will run through completion.

Following completion, the subsequent cycle will begin

automatically.

NOTE: A program can always be stopped (cancelled) by pressing and

P14 50.014003:00

holding the “start/stop” key.

3. Disabling the temperature, speed or time functions.

a) To deactivate the temperature control, press and hold the “” Temp. key until the

set temp. shows “OFF”. The temperature control is now deactivated and the

instrument can be used solely as a shaker/mixer.

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H5000 Series MultiTherm Shakers

p

b) To deactivate the shaker speed, press and hold the “” Speed key until the set

speed shows “OFF”. The shaker is now deactivated and the instrument can be

used solely for temperature control.

c) To deactivate the timer, press and hold the “” Time key until the set time shows

“OFF”. The timer is now deactivated and the instrument will run continuously

until the user presses and holds the start/stop key.

NOTE: When running consecutive programs in the Seq. Link mode, the time should

not be set to “OFF” in any program.

4. Pulse

By pressing and holding the Pulse key, the

instrument mixes at the selected speed for as

long as the Pulse key is held down. The time is

counted in seconds until 999 seconds has

expired.

Pulse is running

600r

m 023S

5. Temperature calibration

The temperature of the Instrument has been factory adjusted and calibrated prior to

being shipped. But if there is deviation between the actual temperature and the displayed

temperature, the user can self-calibrate the instrument in order to correct the error.

Note: The temperature accuracy will be within ±0.5°C.

Calibration Procedure:

a) Verify that the ambient temperature is less than 25°C and power up the instrument.

Once powered on, verify that the current temperature in display is below 25°C. If the

temperature is above than 25°C, please wait for the unit to cool down to 25°C or less.

b) Inject olefin oil into one of the cone-shaped wells, and insert a thermometer into this

well. (Make sure the accuracy of the thermometer is to be within 0.1°C and that it is

completely immersed into well). Heat insulation material is needed on the block to

insulate it from the ambient temperature. See Fig a.:

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H5000 Series MultiTherm Shakers

Adj

Adj

Notes: Please read the actual value after allowing 20 minutes constant temperature

equilibration time.

To enter the temperature calibration mode, press
the “” and “” Prog. Keys simultaneously.

The instrument begins to cool to the low

calibration point.

- H5000-H

Calibration points: 40°C, 100°C

- H5000-HC

Calibration points: 10°C, 40°C, 100°C

c) After 20 mintues，read the temperature of the

Thermometer (for ex. 9.8°C). Using the “”

or “” Temp. keys, adjust the temperature of

the display to match that of the thermometer

and press the “start/stop”key to confirm.

Fig. A

Thermometer

Insulating sheet

Standard Mineral Oil

Well-bottom

P：20.5 ADJ 

Temp= 10.0

P:10.0 ADJ
AdjTemp= 10.0

P:10.0 ADJ 

Temp= 9.8

Press “stop/start”

— 14 —

H5000 Series MultiTherm Shakers

Adj

d) Repeat the above step for the remaining

temperature calibation points.

Following the adjustment to the final calibration point,

the temperature calibration of the instrument has now

been completed and the displayed temperature will

accurately represent the temperature of the heating

block.

P：40.1 ADJ 
AdjTemp= 40.0

P：100.0 Adj 

Temp= 98.0

NOTE: During the temperature calibration, press the “” and “” Prog. keys

simultaneously to cancel the calibration. The system will default back to the former

calibration.

— 15 —

H5000 Series MultiTherm Shakers

6．Changing the block

a) Open the transparent lid and use the

supplied hex wrench (3mm) to remove the

four screws that hold the block to the
heating board.

b) Close the lid and lift the block up and away

from the base unit.

c) Place the desired block softly on the

heating platform of the base unit and
carefully align the four screw holes.

d) Install and tighten the four screws. Do not

over tighten.

Screw

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Chapter 5: Failure analysis and troubleshooting

Failure analysis and processing procedures

No. Symptom Possible Causes Action

No power Check the power

No display when

1

powered up.

The actual and
displayed

2

temperatures are
significantly different.

“OPEN” in the

3

temperature display
with a short beep

“SHORT” in the

4

temperature display
with a shirt beep

5 No heating or cooling

No reaction when a

6

key is pressed

Fuse failure

Broken switch Replace the switch

Others Call for service

Temperature sensor failure Call for service

Temperature sensor failure
or the environmental
temperature is below zero

Temperature sensor failure
or the environmental
temperature is below zero

Broken sensor or Broken TE
module

Failed film switch Call for service

Exchange fuse（250V 3.0A
5X20mm）

Call for service

Call for service

Call for service

Notes

PH: 908-769-5555 EM: [email protected]