Bel-Art Scienceware 37895-1100, 37895-1010, 37895-0100, 37895-0010 User manual

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I N S T R U C T I O N S
V A R I PE T
Repetitive Positive D i s p l a c e m e n t Dispenser
Catalog Numbers
®
F37895-0010 Teflon
F37895-0040 Teflon
F37895-0100 Teflon
F37895-0300 Teflon
Tip 1ml
®
Tip 4ml
®
Tip 10ml
®
Tip 30ml
™
Tip 1ml
™
Tip 4ml
™
Tip 10ml
®
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CO N TE N TS
IMP ORTAN T NOTE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
PRI NCIPL E OF O PERAT ION . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
SPE CIFIC AT ION S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
OPE RAT ION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
SET V OLU ME . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
FIL L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
ELI MINATE A IR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
DIS PENSE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
CLE ANING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
AUT OCLAVIN G . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
ASS EMBLY AN D DI SA SSE MBLY . . . . . . . . . . . . . . . . . . . . . . . .4
VA RIPE T VA RIPE T VA RIPE T
CHE MICA L COMPATIB ILIT Y CH ART . . . . . . . . . . . . . . . . . . . . . 7, 8
Consult Chemical Resistance Chart before using your Varipet®to determine that it is compat­able with the chemical to be dispensed.
All parts of the Teflon
®
Teflon are chrome plated brass and stainless steel.
Each Varipet of accuracy and reliability. There may be a small amount of ordinary tap water remaining in the barrel as a result of the testing process.
®
TRO UBLES HOOT ING . . . . . . . . . . . . . . . . . . . . . . . . .4
®
1 TO 10m l DIAG RAM . . . . . . . . . . . . . . . . . . . . . . . . .5
®
30m l DIA GRAM . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
I M P OR TA N T NO T E
®
Tip Varipet® that come in contact with liquid are made of glass or
, and are suitable for use with most acids and solvents. Metal Luer-Lok™and cannula
®
is tested before it is shipped to assure that it performs to the highest standards
PRINCIPLE OF OPERATI ON
Varipet®is a variable volume pipettor for repetitive dispensing of preset amounts of liquid. Set the desired volume, then depress the plunger for dispensing and release it for filling.
SPECIFI CATIONS
(Size) Maximum Volume: 1ml 4ml 10ml 30ml
Graduation: 0.01ml 0.05ml 0.2ml 1ml Repetitive accuracy ±%
at full scale: 0.75 0.5 0.5 1.0 at half scale: 1.0 0.75 0.75 1.5 at quarter scale: 1.5 1.0 1.0 1.0
Statistical accuracy within two standard deviations (97% of all readings fall within these lim­its).
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OPERATION
TO SET VOLUME
1. Rotate knob clockwise to decrease volume; counter-clockwise to increase volume.
2. Line up bottom edge of piston with the appropriate graduation on the glass barrel.
3. Dispense set volume in graduated beaker or cylinder. Check and readjust if necessary.
TO FILL
1. Depress plunger fully.
2. Insert tip of Varipet
3. Release plunger. The Varipet
®
is now full and ready for dispensing.
®
in the liquid.
TO ELIMINATE AIR
Should air bubbles appear around piston, the following corrective action should be taken:
1. Depress plunger quickly and forcefully several times using short strokes while tip is in liquid.
2. Turn pipette tip end up and tap glass barrel until air bubbles rise to the top. Then slowly depress plunger until air is expelled.
3. Make sure glass barrel and plunger are clean.
TO DISPENSE
Depress plunger fully. Insert tip of Varipet®into the liquid to be transferred before releasing plunger. This method should prevent air from entering the system.
CLEA NING
1. Empty Varipet®by removing tip from the liquid and pressing knob a few times.
2. Insert tip in cleaning solution. Depress plunger several times to fill and then empty unit.
3. Use only chemicals for this purpose that are compatible with the specific model. For general cleaning, use detergent (such as Bel-Art’s Aquet
F17094-0030) or chromic-sulphuric acid solution (made with Bel-Art’s Chromerge
4. Harsh corrosive chemicals should not be left in pipette for extended periods of time.
®
, Cat. No. F17089-0000) for more stubborn deposits.
Clean and rinse it immediately after each use.
®
, Cat. No.
AUTO CLAVI NG
All parts of Varipet®are completely autoclavable. However: it is necessary to sepa- rate plunger assembly from glass barrel for autoclaving. Follow standard autoclaving prac­tices.
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ASSE MBLY AND DISA SSEMBLY
TO DISASSEMBLE (1, 4, 10ml MODELS)
Hold plastic syringe adapter (93790-1109 1ml) (93790-0106, 4ml) or glass barrel (10ml) in one hand and plastic body in the other hand. Twist a quarter turn in either direction, then pull the upper piston assembly out of glass barrel.
TO REASSEMBLE (1, 4, 10ml MODELS)
Insert piston into glass barrel tilted at an angle so as not to damage piston edges. Once pis­ton is inside barrel it can be pushed straight in to complete assembly. While rotating plastic adapter (93790-1109, 1ml) (93790-0106, 4ml) or glass barrel (10ml) push both parts together until the adapter or barrel flange disappears into the plastic body; then rotate a quarter turn in either direction to lock. A rotating motion of the glass barrel in the locked position will not affect the operation of the Varipet
®
.
TO DISASSEMBLE 30ml MODEL
Remove flange screws holding plunger assembly and glass barrel together. Separate plunger assembly from glass barrel by pulling them apart.
TO REASSEMBLE 30ml MODEL
Insert piston into glass barrel tilted at an angle so as not to damage piston edges. Once pis­ton is inside barrel, it can be pushed straight in to complete assembly. Line up screw holes in flange and reinsert screws. Tighten until snug. Do not overtighten.
®
VARIPET
TROU BLE SHOOTING
KNOB DIFFICULT TO TURN WHEN SETTING VOLUME
No lubrication on adjustment nut (Cat. No. 93790-1103 or 93790-0139). Remove upper piston assembly from glass barrel. Turn knob clockwise until nut appears.
Lubricate nut with petroleum jelly and reassemble.
TO CORRECT PISTON LEAKS
When liquid passes behind the Teflon®piston it is because the Teflon®plunger and the glass barrel are no longer forming a proper seal.
FOR 1ml MODELS, F37895-0010 & F37895-1010
1. Disassemble the unit by removing the plunger assembly from the glass barrel.
2. Insert a blunt object like a ballpoint pen in the front face groove of the plunger.
3. Gently force the thin Teflon circumference. If the Teflon®edge is intact and has no nicks, this simple operation will restore the sealing ability of the plunger. If not, replace the piston and pin (#93790-
1120).
®
edge outward by circling the pen around the entire
FOR ALL OTHER MODELS
1. Disassemble the unit by removing the plunger assembly from the glass barrel.
2. Closely inspect the plunger; if there is a nick in the plunger, the piston and rod should be replaced. See diagrams for replacement #.
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VA RI PE T®D I A G R AM
1 m l, 4 m l, 1 0 ml Si z es
Knob 93790-1131
Main Spring 93790-1128
Adjustment Nut 93790-1103
1ml Syringe Adapter 93790-1109 or 4ml Syringe Adapter 93790-0106
Syringe Barrel or Teflon
1ml w/Luer-lok™93790-1102 or 1ml w/Teflon®Tip 93789-5001
4ml w/Luer-lok™93790-0104 or 4ml w/Teflon
10ml w/Luer-lok™93790-0110 or 10ml w/Teflon®Tip 93789-5100
®
Tip
®
Tip 93795-0040
Thumb Knob 93790-1130
Bushings 93790-1132
Upper Body
93790-1137
Friction O Ring
93790-1105
E-Ring pk/2
93790-1126
Syringe Barrel O
Ring
93790-1104
Piston & Rod 1ml 93790-1121 4ml 93790-0117 10ml 93790-0120
Piston & Pin 1ml 93790-1120 4ml 93790-0116 10ml 93790-0119
Tef l o n®Tip
Cannula
Cannula or Teflon Teflon®Tip 93789-5003 16 Ga Cannula 1ml & 4ml 93790­1116 13 Ga Cannula 10ml 93790-0111
®
Tip
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Luer-Lok
™
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VA RI PE T®D I A G R AM
Knob 93790-1131
Main Spring 93790-0145
Clamp Flange 93790-0136
3 0 ml Si z e
Thumb Knob
93790-1130
Bushing
93790-0140
Adjustment
Nut
93790-0139
Piston & Rod
Assembly
93790-0131
Piston & Pin
93790-0144
Syringe Barrel
30ml Syringe
Only
93790-0143
w/ Teflon
93789-5030
6
®
Tip
®
Teflon
93789-5032
Tip
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CHEMICAL COMPATIBILITY CHART
CONSULT THIS CHART TO DETERMINE THE VARIPET
®
SUITED TO YOUR REQUIREMENTS
This chart contains information gained from laboratory tests and should be used as
a guide only. For applications not shown, consult our Engineering Department.
Harsh corrosive chemicals should not be left in Varipet
®
for extended periods of time.
Clean Varipet®immediately after each use
Chemical
ACETIC ACID (5%) YES ACETIC ACID (60%) YES ACETIC ACID (Glacier) YES ACETIC ANHYDRIDE YES YES ACETONE YES ALCOHOLS in general YES YES ALUMINUM CHLORIDE SOLUTIONS YES YES ALUMINUM SULFATE SOLUTIONS YES YES AMMONIA YES YES AMMONIUM CHLORIDE SOLUTIONS YES YES AMMONIUM HYDROXIDE (58%) YES YES AMMONIUM PERSULFATE (Set'd) YES AMMONIUM PHOSPHATE YES AMMONIUM SULFIDE YES ANIUNE YES BARIUM HYDROXIDE SOLUTIONS YES YES BENZENE YES BORIC ACID YES YES BUTANOL YES YES BUTYL ACETATE YES CADMIUM CHLORIDE YES CALCIUM CHLORIDE YES YES CALCIUM HYPOCHLORITE YES CARBON TETRACHLORIDE YES CHLOROFORM YES CHLOROACETIC ACID YES CHROMIC ACID (10%) YES CHROMIC ACID (50%) YES CITRIC ACID (10%) YES YES COPPER CHLORIDE SOLUTIONS YES COPPER SULFATE SOLUTIONS YES YES CELLOSOLVE YES YES CRESOL YES CYCLOHEXANONE YES DIACETONE ALCOHOL YES DIBUTYL PHTHALATE YES DIBENZYL SEBACATE YES DIISOBUTYL KETONE YES ESTERS in general YES ETHERS in general YES ETHYL ACETATE YES ETHYL BENZENE YES ETHYLENE GLYCOL YES ETHYL ETHER YES FATTY ACIDS YES FERRIC CHLORIDE SOLUTIONS YES FERRIC NITRATE SOLUTIONS YES FERRIC SULFATE YES FORMALDEHYDE (40%) YES YES FORMIC ACID (100%) YES YES FUEL OIL YES YES GASOLINE YES GLUCOSE YES YES GLYCERINE YES YES HEPTANE YES
Compatability
Luer-Lok
Varipet
™
Teflon®Tip
®
Varipet
®
Chart continued on next page
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Chemical
N-HEXANE YES HYRAULIC OIL YES YES HYDROCHLORIC ACID (10%) YES HYDROCHLORIC ACID (37.5%) YES HYDROGEN PEROXIDE YES ISOOCTANE YES YES ISOPENTYL ACETATE YES KEROSENE YES YES KETONES in general YES LACTIC ACID (85%) YES YES MAGNESIUM CHLORIDE YES YES METHYL ALCOHOL YES YES METHYL BENZOATE YES METHYL ETHYL KETONE YES METHYL ISOBUTYL KETONE YES NITRIC ACID (10%) YES NITRIC ACID (50%) YES OLEIC ACID YES OXALIC ACID YES PALMITIC ACID YES YES PERCHLORIC ACID (68%) YES PHOSPHORIC ACID (75%) YES YES PHOTOGRAPHIC DEVELOPERS YES PHOTOGRAPHIC FIXING SOLUTIONS YES POTASSIUM CHLORATE YES YES POTASSIUM HYDROXIDE (9%) YES YES POTASSIUM HYDROXIDE (conc.) YES YES POTASSIUM PERMANGANATE YES YES SAE 20 OIL YES YES SODIUM HYDROXIDE (conc.) YES SODIUM HYPOCHLORITE YES STANNOUS CHLORIDE YES YES STEARIC ACID YES YES SULFURIC ACID (10%) YES SULFURIC ACID (50%) YES SULFURIC ACID (75%) YES TANNIC ACID YES YES TARTARIC ACID YES YES TOLUENE YES TRICHLOROACETIC ACID YES TRICHLOROETHYLENE YES TRICRESYL PHOSPHATE YES YES TRIETHANOLAMINE YES YES TURPENTINE YES YES XYLOL (Xylene) YES ZINC CHLORIDE YES YES
Luer-Lok
Varipet
™
Teflon®Tip
®
Varipet
Compatability
®
CHEMICAL COMPATIBILITY CHART
CONSULT THIS CHART TO DETERMINE THE VARIPET
SUITED TO YOUR REQUIREMENTS
This chart con tai ns information gained from laboratory t est s and sh oul d be used as
a guide only. For applicat ion s not sh own , consult our Engineering Department.
Harsh corrosive c hem ica ls should not be left in Varipet®for extended p eri ods of t ime.
Clean Varipet
937895009 07/08
Scienceware registered TM of BD • Bel-Art Products assumes no obligation or liability for any advice furnished by it, or for results obtained with respect to these products. All such advice is given and accepted at the buyer’s risk. ©2008 Bel-Art Products. All Rights Reserved.
®
, Varipet®are registered TMs of Bel-Art Products • Teflon®is a registered TM of E.I. duPont de Nemours & Co. • Luer-Lok™is a
®
immediately after ea ch use.
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®
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