Becton Dickinson CA6000 User guide

CLAY ADAMS

™

CA

Centrifuge Users

BECTON DICKINSON AND COMPANY

Becton Dickinson Microbiology Systems

6000

Manual

7 Loveton Circle

Sparks, Maryland 21152 USA

18006318064

March, 1998

Document Number: MA0128

Revision: New

Catalog Number: 42601025

CA6000 Centrifuge Users Manual

Clay Adams is a registered trademark of Becton Dickinson and Company.

© Copyright Becton Dickinson and Company, 1998. All rights reserved. No part of this publication may be
reproduced, transmitted, transcribed, stored in retrieval systems, or translated into any language or
computer language, in any form or by any means, electronic, mechanical, magnetic, optical, chemical,
manual, or otherwise, without the prior written permission of Becton Dickinson Microbiology Systems, 7

Loveton Circle, Sparks, Maryland, 21152, United States of America.

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CA6000 Centrifuge Users Manual

Contents

1  Introduction

1.1 Overview  Functional Principles ....................................................................................... 1

1.2 Safety Devices and Notices .............................................................................................. 3

1.3 Hazards, Precautions, and Limitations of Use ................................................................... 3

1.4 Rotor and Accessory Precautions ..................................................................................... 4

1.4.1 Corrosion Information ........................................................................................... 4

1.5 Contamination Hazards..................................................................................................... 5

1.6 Manual Structure............................................................................................................... 6

1.7 Use of this Manual ............................................................................................................ 6

1.8 Manual Conventions .......................................................................................................... 6

1.8.1 Symbols Used on the Equipment ......................................................................... 6

1.8.2 Notes, Cautions, and Warnings............................................................................ 7

2  Installation and Setup

2.1 Transport, Packaging, Lifting............................................................................................. 8

2.2 Installation ........................................................................................................................ 8

2.2.1 General Placement .............................................................................................. 8

2.2.2 Inspection ............................................................................................................ 9

2.2.3 Electrical Connection ........................................................................................... 9

2.2.4 Lid Unlock ............................................................................................................ 9

2.2.5 Assembly of the Rotating Head ............................................................................ 9

2.3 Specifications ................................................................................................................. 10

3  Controls and Indicators

3.1 General ........................................................................................................................... 12

3.2 On/Off Switch ................................................................................................................. 12

3.3 Lid Release Latch ........................................................................................................... 13

3.4 Emergency Lid Release Access Screw........................................................................... 13

3.5 CA6000 Keypad .............................................................................................................. 13

3.5.1 RPM Readout .................................................................................................... 13

3.5.2 RPM Set Keys ................................................................................................... 13

3.5.3 Not In Use Indicator ........................................................................................... 14

3.5.4 In Use Indicator .................................................................................................. 14

3.5.5 Time (MIN) Readout ........................................................................................... 14

3.5.6 Time Set keys ................................................................................................... 14

3.5.7 SOFT ACCEL Key ............................................................................................. 14

3.5.8 SOFT ACCEL Indicator ...................................................................................... 14

3.5.9 SOFT BRAKE Key ............................................................................................. 15

3.5.10 SOFT BRAKE Indicator...................................................................................... 15

3.5.11 START key ......................................................................................................... 15

3.5.11 STOP/PROGRAM LOCK Key ............................................................................ 15

3.5.12 PROGRAM Keys ............................................................................................... 15

3.5.13 Program Indicators ............................................................................................. 15

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CA6000 Centrifuge Users Manual

Contents

4  Operation

4.1 CA6000 Centrifuge Setup for Operation ........................................................................... 16

4.2 Loading Balance ............................................................................................................. 17

4.3 Rotating Equipment Performance .................................................................................... 18

4.4 Selecting Operating Modes ............................................................................................. 18

4.5 Starting the Centrifuge..................................................................................................... 20

4.6 Stopping the Centrifuge ................................................................................................... 20

4.7 Emergency Lid Unlock .................................................................................................... 20

5  Maintenance

5.1 Disassembly of the Rotating Head .................................................................................. 21

5.2 Rotating Equipment and Centrifugation Chamber Cleaning .............................................. 21

5.3 Exterior Cleaning ............................................................................................................ 22

5.4 Disinfection ..................................................................................................................... 22

5.5 Radioactive Decontamination .......................................................................................... 23

5.6 Fuses ............................................................................................................................. 23

6  Troubleshooting

6.1 Alarm Messages ............................................................................................................. 24

6.2 Other Problems .............................................................................................................. 25

6.3 Repair ............................................................................................................................. 25

A  Limited Warranty

B  Parts and Accessories

C  International Contacts

D  Exploded View Drawing

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CA6000 Centrifuge Users Manual

Figures

1 Clay Adams CA6000 Centrifuge..................................................................................... 2

2 Symbols Used on the CA6000 Centrifuge ......................................................................... 6

3 CA6000 Centrifuge Layout .............................................................................................. 13

4 CA6000 Centrifuge Keypad ............................................................................................. 14

5 Loading Balance ............................................................................................................. 17

Tables

1 Physical Dimensions ...................................................................................................... 10

2 Environmental Requirements........................................................................................... 10

3 Electrical Requirements ...................................................................................................11

4 Centrifugation Characteristics ..........................................................................................11

MA0128 v

Introduction



1.1 Overview  Functional Principles

The Clay Adams CA6000 centrifuge is a multipurpose centrifuge designed for laboratory use in
performing separation and sedimentation procedures. Through the application of a Relative Centrifugal
Force (RCF) it allows the separation of substances made of different density elements. Potential
applications include hematology, chemistry, urinalysis, blood banking, microbiology, cytology, pharma­cology, food processing, and agriculture.

Specific features include:

H Microprocessor controlled

H 5 Program (Memory) Settings

H Speeds from 300  6500 rpm in 10 rpm increments

H High or low acceleration (5 preprogrammed settings)

H High or low deceleration (4 preprogrammed settings plus inertial)

H Timer from 1 minute to 99 minutes plus infinite state

H Electronic-accelerometric imbalance encoder

H Direct drive system

H Brushless induction drive motor

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CA6000 Centrifuge Users Manual

Drive System

The centrifuge is driven by a three phase asynchronous motor. It is situated in the center of a sealed
and armor plated centrifugation chamber.

Safety Interlock System

The CA6000 centrifuge is equipped with an interlock system that prevents opening of the centrifuge lid
when the rotor is spinning. The centrifuge will not operate until the lid is closed and latched in place.
The lid remains latched until the rotor stops spinning.

NOTE

If a power failure occurs, you can access the samples in the centrifuge by

following the Emergency Lid Unlock procedure in Section 4.7.

Imbalance Sensor

The centrifuge is equipped with a load imbalance detector. In case of excessive imbalance the machine
stops and displays the message IMBAL. After the lid has been opened to remove the cause of the
imbalance, the message disappears. Carefully balance the sample load as described in Section 4.2
to avoid activating the imbalance detection system.

Figure 1  Clay Adams CA6000 Centrifuge

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CA6000 Centrifuge Users Manual

1.2 Safety Devices and Notices

In order to ensure both mechanical and electrical operational safety, the centrifuge features the following
safety control systems:

F The centrifuge will not start unless the lid is locked. The lid cannot be opened while the rotor is

spinning, even in the event of power failure.

F Safety electronic circuits for self-diagnosis (with microprocessor control); any possible breakdown

of the circuits and of the sensors is shown on the display and neutralized, preventing unsafe
operating conditions.

F Load imbalance detection.

F Double metal protection casing; reinforced centrifugation chamber in AISI 304 stainless steel.

F Lid anti-fall mechanism.

F Main bipolar switch.

F Normalized filters to avoid Radio Frequency Interference (RFI).

F Individual certified test, with measurements and testing of the leakage currents, the ground

resistance, and the applied voltage.

F Identification plates; operating instructions.

1.3 Hazards, Precautions, and Limitations of Use

The following CAUTIONS and WARNINGS represent dangerous operations and work conditions to avoid.

! Using the centrifuge if it has not been properly installed.

! Leaning on the machine.

! Depositing any object in the free area of the centrifuge bowl.

! Using the centrifuge with rotating equipment showing evident corrosion, wear marks, or cracking

on the rotating head or on the buckets.

! Using the centrifuge with rotating equipment and accessories not approved by Becton Dickinson.

! Using the centrifuge in explosion risk rooms, or with explosion risk products or chemical materials

subject to violent reaction.

! Incorrect assembly of the rotating head on the driving stud.

! Imbalanced load causing an excessive vibration of the centrifuge.

! Failing to insert all buckets into the rotating equipment, even for partial loads.

! Moving or shifting the machine during centrifugation.

WARNING

IF ANY DANGEROUS EVENT OCCURS INVOLVING THE CENTRIFUGE,

VACATE THE AREA AND SWITCH OFF THE MAIN POWER SOURCE.

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CA6000 Centrifuge Users Manual

! Exceeding the maximum speed indicated in the max. rpm column of the performance table (see

4.2.2).

! Using old accessories on a new machine.

! Using tubes or bottles not suited for centrifugation.

! Centrifuging samples with higher density than allowed for the maximum speed, without reducing

the speed limit (see Section 4.2).

! Manipulating or tampering with the electronic and mechanical parts.

1.4 Rotor and Accessory Precautions

1.4.1 Corrosion Information

CA6000 rotors made of aluminum alloy are designed to operate at their rated RCF for many years. With
careful use they will resist corrosion, lessening the possibility of excessive imbalance, disruption and
subsequent damage to the instrument. The primary conditions for the initiation of corrosion exist in every
laboratory during daily use of the centrifuge. For this reason it is essential that care and attention be paid
to inspection and cleaning.

CHEMICAL CORROSION

This corrosion is characterized by chemical reactions due to the existence of any electrolyte liquid on
the surface of the rotating equipment. If these substances are allowed to remain on the surface, the
corrosion will almost certainly occur. This produces first a discoloration of the anodization and
eventually pitting of the metal.

Acidic and alkaline solutions sustaining their pH level will create problems of corrosion in aluminum
equipment. Chlorides, present in salts or even in skin contact with the rotating equipment, are among
the most aggressive and harmful substances commonly found in the laboratory.

Chemical products, which are the source of this corrosion do not necessarily originate from broken
tubes, for example they could come from:

H Chemical vapors present in the laboratory which are dissolved in the residual humidity, in

condensed water (refrigerated centrifuges) present at the base of the rotor pockets.

H Corrosive liquids originating from overfilled uncapped tubes (the liquid overflowing during

centrifugation)

H Inserts, adapters, racks, bottles whose exterior has been soiled by a chemical product or

poorly rinsed after decontamination (with bleach, for example)

NOTE

If the products are very corrosive, simply rinsing is insufficient. Residual

traces dissolve little by little with the humidity present in the bottom of the

rotor pocket.

CAUTION

BEWARE of the presence of solid particles beneath tubes, inserts,
racks, or adapters. These particles are crushed by the centrifugal

force and penetrate the protective, anodized layer of buckets and

rotors, thus creating easy pathways for corrosion.

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STRESS CORROSION

This term relates to the phenomenon of accelerated corrosion due to the effect of centrifugation when
a corrosive chemical is in contact with the alloy. From the time when the aluminum alloy has been
attacked by chemicals, stress corrosion begins to appear. Because the process begins on a
microscopic scale it is even more dangerous than a macroscopic process since it is invisible.

During centrifugation, chemicals responsible for corrosion are also submitted to the very high g force
which pushes them against the alloy. This close contact facilitates the chemical reaction which occurs
much faster than in a static situation. Moreover, centrifugal force is very directional thus corrosion
under stress creates, with a very small amount of corrosive product, straight microscopic fissures.
Each centrifugation run makes the chemical migrate further and further.

Fissures or cracks, although microscopic, are cuts in the metal, breaking the materials cohesion. As
one weak link in a chain allows the chain to break, so the microfissures break the chain of resistance
of the accessory to centrifugal force. As accessories are designed with high levels of safety, rupture
does not occur as soon as the first microfissures are produced.

Depending upon the location of the fissure, disruption may occur before it reaches the external surface
of the accessory. The fissure creates a weakness which makes the accessory less and less resistant
to mechanical fatigue. The corrosion by a small amount of corrosive product does not disrupt the
accessory but makes it mechanically weaker and weaker until disruption occurs due to both
centrifugal effort and the number of cycles.

Because stress corrosion is largely invisible, it is essential that rotating equipment be
scrutinized regularly paying particular attention to susceptible parts such as the bottom of
pockets, the outer edges and the base of the rotating equipment.

1.5 Contamination Hazards

Clay Adams centrifuges are likely to be used in facilities where hazardous substances (including
radioactive chemicals) are frequently present.

WARNING

BLOOD AND BODY FLUIDS MAY CONTAIN THE HEPATITIS B VIRUS

(HBV), HEPATITIS C VIRUS (HCV), HUMAN IMMUNODEFICIENCY VIRUS

(HIV), OR OTHER DISEASE-CAUSING AGENTS. HANDLE ALL PATIENT

SPECIMENS AS POTENTIAL BIOHAZARDS CAPABLE OF TRANSMIT-

TING INFECTION. WEAR APPROPRIATE PERSONAL PROTECTIVE

EQUIPMENT, INCLUDING LABORATORY GLOVES, WHEN COLLECT-

ING, HANDLING, AND PROCESSING BLOOD AND BODY FLUIDS.

IN ADDITION TO WEARING GLOVES, THE USE OF DISPOSABLE

LAB COATS OR GOWNS AND PROTECTIVE GLASSES OR

GOGGLES IS RECOMMENDED WHEN WORKING AROUND THE

CENTRIFUGE.

IF A TUBE BREAKS IN THE UNIT, CAREFULLY REMOVE BROKEN

GLASS WITH A HEMOSTAT OR OTHER DEVICE, USING PUNCTURE

RESISTANT UTILITY GLOVES.

Always use the appropriate decontamination procedures when the rotating equipment is exposed to these
chemicals. Examples of commonly used techniques are outlined in Section 5  Maintenance. The
information is given as a guide only. Decontamination is the responsibility of the owner to use the most
suitable procedure.

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CA6000 Centrifuge Users Manual

!

The rotating equipment should always be completely disassembled before being subjected to heat and after
external chemical cleaning. Seals, tubes, and plastic components should be decontaminated with the
method most suitable for them (which might not be the same as for the rotating equipment).

1.6 Manual Structure

This users manual contains the following sections:

Section 1  Introduction  provides an overview of the CA6000 centrifuge and its uses in the laboratory.

Section 2  Installation and Setup  gives specifications for installation of the centrifuge and

instructions for setup.

Section 3  Controls and Indicators  explains the use and meaning of all controls and indicators

of the unit.

Section 4  Operation  provides instructions for routine daily activities.

Section 5  Maintenance  explains all user system maintenance, including parts replacements.

Section 6  Troubleshooting  provides a convenient guide for identifying and correcting system

malfunctions.

The Appendices contain warranty information, parts and accessories list, a listing of Becton Dickinson

international contacts, and an exploded view drawing.

1.7 Use of this Manual

This users manual is designed as a reference tool for technologists, supervisors, and other personnel who
operate and maintain the CA6000 centrifuge on a regular basis. Every attempt has been made to include
all information which would be required during normal use and maintenance. Should a question arise which
is not answered in this manual, please contact the following parties (USA):

For assistance with mechanical or electrical performance problems:

( Field Service 18005447434

For procedural questions:

( Technical Services 18006318064

International contacts are listed in Appendix D.

1.8 Manual Conventions

1.8.1 Symbols Used on the Equipment

Figure 2  Symbols Used on the CA6000 Centrifuge

Left figure: Symbol for electrical hazard; Middle figure: Symbol for refer to accompanying

documentation (specifically, the users manual) for instructions; Right figure: Symbol for fuse.

MA0128 6

CA6000 Centrifuge Users Manual

1.8.2 Notes, Cautions, and Warnings

Throughout this manual, important information is presented in boxes offset from the regular text, and is
labeled as either a NOTE, CAUTION, or WARNING. These messages are formatted as shown below and
bear the following significance:

NOTE

Important information about system use worthy of special attention is

presented as a NOTE.

CAUTION

Information on an activity which potentially could cause damage to

the centrifuge is presented as a CAUTION.

WARNING

INFORMATION ON AN ACTIVITY WHICH POTENTIALLY COULD
CAUSE INJURY TO THE USER IS PRESENTED AS A WARNING.

MA0128 7

Installation and Setup



2.1 Transport, Packaging, Lifting

Due to the weight of the CA6000 centrifuge, all lifting and transporting must be carried out using proper handling
equipment (e.g., forklift) that complies with current regulations, and by people with the necessary training.

The centrifuge may be transported and stored in ambient temperatures from 20° C to 50° C, and relative
humidity up to 90% (non-condensing).

During transport the centrifuge is seated in a special packaging. Unpack the centrifuge, carefully removing
first the rotating equipment, any possible accessories and the material supplied for ordinary maintenance.
Check the contents of packaging against the packing list and note any damage to the shipping carton. If
damage is observed, notify the carrier immediately.

Keep the packaging until the centrifuge has been set up and tested.

WARNING

DUE TO ITS SIZE AND WEIGHT, AT LEAST TWO PEOPLE ARE

REQUIRED TO LIFT THE CENTRIFUGE FROM THE PALETTE AND

PLACE IT ON THE BENCH.

2.2 Installation

2.2.1 General Placement

The centrifuge should be installed in a dust and corrosion free room.

Place the centrifuge on a bench top capable of supporting the specified weight (see Section 2.3). Be sure
there is approximately 12 in. of clearance on all sides of the unit.

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CA6000 Centrifuge Users Manual

2.2.2 Inspection

Before installation, the centrifuge should be thoroughly inspected for corrosion and cleanness (see Section
5  Maintenance). The clip in the center of the rotor and the drive spindle should also be clean and

undamaged: these parts should be wiped over before each use.

Any failure to follow the above advice may have serious consequences for the safety of the appliance.

2.2.3 Electrical Connection

Attach the power cord to the receptacle on the rear panel of the centrifuge. Plug the power cord into the
appropriate 3-wire grounded power receptacle. If an extension cord is required, use only a 3-wire grounded
cord rated for the correct voltage for your unit (120 VAC/15 A or 220 VAC/7.5 A).

WARNING

TO AVOID ELECTRICAL SHOCK, CONNECT THE POWER CORD ONLY
TO AN APPROVED POWER SOURCE SUCH AS A 3-WIRE GROUNDED

RECEPTACLE. WHERE A 2-WIRE RECEPTACLE IS ENCOUNTERED,

HAVE IT REPLACED WITH A PROPERLY GROUNDED 3-WIRE RECEP-

TACLE IN ACCORDANCE WITH THE NATIONAL ELECTRICAL CODE.

DO NOT, UNDER ANY CIRCUMSTANCES, REMOVE THE GROUND

PRONG FROM THE POWER PLUG.

SHOULD THE POWER CORD OR PLUG BECOME CRACKED, FRAYED,

BROKEN, OR OTHERWISE DAMAGED, REPLACE THE CORD OR

UNPLUG THE POWER CORD BEFORE ANY MAINTENANCE OR SERVICING.

NEVER ATTEMPT TO OVERRIDE THE ELECTRICAL SAFETY INTER-

2.2.4 Lid Unlock

1 Switch the centrifuge on by flipping the power switch on the right side of the machine to the on

position.

2 When the centrifuge is switched on and not spinning the NOT IN USE LED will be lit.

3 To open the lid, pull the lever on the right side of the machine towards the centrifuge front.

BEFORE PROCEEDING, REMOVE POWER TO THE CENTRIFUGE BY

4 After opening the lid, remove any packaging around the driving spindle. Unscrew the rotor head nut

from the motor shaft. Carefully clean the inside of the centrifuge chamber removing any packing
residues. Due to the air-whirling, solid particles accidentally left in the centrifuge chamber would
create excessive wear of the chamber itself and of the rotors.

PLUG IMMEDIATELY.

LOCKS OF THE CENTRIFUGE.

WARNING

MOVING THE POWER SWITCH TO THE OFF POSITION.

2.2.5 Assembly of the Rotating Head

The rotors drive is made by means of two pins inserted in the rotors shaft which must be aligned with the
milling of the rotor. For a correct mounting follow the instructions below:

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CA6000 Centrifuge Users Manual

 Ensure that both the spindle of the motor and the hole of the head are clean. It is suggested that

you apply a light coat of lubrication with a light oil or silicone spray.

 Insert the head in the centrifuge protective bowl and slip it onto the spindle with the milling of the

collar face down.

 Align the milling in the collar with the pins on the spindle.

Screw and slightly tighten (with the supplied key) the hexagonal nut on the threaded spindle of the motor
unit.

2.3 Specifications

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Table 1  Physical Dimensions

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Table 2  Environmental Requirements

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CA6000 Centrifuge Users Manual

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Table 3  Electrical Requirements

The centrifuge is provided with RFI Filters. The manufacturer declines all responsibility for any
damages due to non-grounding of the centrifuge.

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Table 4  Centrifugation Characteristics

MA0128 11

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Controls and Indicators



3.1 General

This section describes the meaning and use of the controls and indicators of the Clay Adams CA6000
centrifuge. The overall layout of the unit is shown in Figure 3. The keypad is shown in Figure 4.

WARNING

ALL SYSTEM USERS MUST BECOME THOROUGHLY FAMILIAR WITH

ALL CONTROLS AND INDICATORS BEFORE ATTEMPTING TO

OPERATE THE CENTRIFUGE.

3.2 On/Off Switch

The On/Off switch is located on the right side of the centrifuge, at the bottom. When in the | (On) position,
power is applied to the centrifuge. When in the O (Off) position, power is removed from the centrifuge.

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CA6000 Centrifuge Users Manual

Figure 3  CA6000 Centrifuge Layout

3.3 Lid Release Latch

The Lid Release Latch is located on the right side of the centrifuge, at the top front. Pull this latch forward
to open the centrifuge lid.

Note that the Lid Release Latch does not operate when power to the unit has failed.

3.4 Emergency Lid Release Access Screw

The emergency Lid Release Access Screw is located on the right side of the centrifuge, just in front of the
Lid Release Latch. In the event of a power failure, remove the screw to access the emergency lid release
function (see Section 4.7).

3.5 CA6000 Keypad

The keypad is located at the center of the front panel. It presents all the keys and displays necessary to
program, start, and stop operation of the centrifuge.

3.5.1 RPM Readout

When the centrifuge is stopped, the RPM Readout displays the speed setpoint (in revolutions per minute).
It displays the actual speed when the centrifuge is spinning. When the readout is flashing, it indicates that
the current speed/time setting can be entered into memory (Program 1  5).

3.5.2 RPM Set Keys

Press p to increase or q to decrease the speed setting. The speed can be set from 300 to 6500 rpm
in 10 rpm increments.

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CA6000 Centrifuge Users Manual

3.5.3 NOT IN USE Indicator

When lit, this amber LED indicates that the rotor is not spinning and the lid can be opened.

3.5.4 IN USE Indicator

When lit, this amber LED indicates that the rotor is spinning and the lid cannot be opened.

3.5.5 Time (MIN) Readout

When the centrifuge is stopped, the time readout displays the time setpoint (in minutes). It displays the
remaining time when the centrifuge is spinning. When the readout is flashing, it indicates that the current
speed/time setting can be entered into memory (Program 1  5).

3.5.6 Time Set keys

Press p to increase or q to decrease the time setting. The time can be set from 1 minute to 99 minutes.
The Hold (Hd) setting, located at the zero position, allows you to manually start and stop the centrifuge
without presetting a time (i.e., spin for an indeterminate or infinite time).

3.5.7 SOFT ACCEL Key

This key is used to select soft acceleration modes. Press and hold the key until the RPM Readout shows
ACL flashing and a number from 1  5. Use p to increase or q to decrease the acceleration profile
number. Profile 1 is the slowest setting, increasing to Profile 5, the fastest setting.

3.5.8 SOFT ACCEL Indicator

This green indicator flashes when the centrifuge is accelerating to its setpoint. The LED remains on while
the actual speed is at the setpoint.

Figure 4  CA6000 Centrifuge Keypad

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CA6000 Centrifuge Users Manual

3.5.9 SOFT BRAKE Key

This key is used to select soft deceleration modes. Press and hold the key until the RPM Readout shows
dCL flashing and a number from 0  4. Use p to increase or q to decrease the deceleration profile
number. Profile 0 represents a coast mode (no braking).

3.5.10 SOFT BRAKE Indicator

This green indicator flashes when the centrifuge is decelerating from its setpoint. The LED remains on while
the actual speed is at the setpoint.

3.5.11 START key

Press to start a centrifuge cycle.

3.5.11 STOP/PROGRAM LOCK Key

Press this key to stop the centrifuge during its spin cycle.

Press this key ALONG WITH a PROGRAM Key to lock the program setting. When a program is locked,
it cannot be changed unless it is unlocked.

3.5.12 PROGRAM Keys

When the RPM and Time (MIN) readouts are flashing, pressing one of the five PROGRAM keys saves the
current speed/time settings in memory. When RPM and Time readouts are not flashing, pressing
PROGRAM 1  5 recalls the speed/time settings stored in that memory location.

You can lock any of the programs while you are setting it by pressing the STOP/PROGRAM LOCK Key
along with the desired PROGRAM Key. When locked, a program cannot be changed. To unlock a program,
press the STOP Key along with the appropriate PROGRAM Key.

3.5.13 PROGRAM Indicators

When a preset program (1  5) is selected, the amber LED next to the PROGRAM number lights.

MA0128 15

Operation



WARNING

USE ONLY THE ROTORS, BUCKETS, INSERTS, AND OTHER ACCES-

SORIES SPECIFIED IN APPENDIX B  PARTS AND ACCESSORIES.

DO NOT OPERATE THE CENTRIFUGE WITH SPECIMENS OR MATE-

RIALS HAVING A DENSITY GREATER THAN 1200 KG/M3. DOING SO

MAY CAUSE DAMAGE TO THE CENTRIFUGE, AND MAY CAUSE

UNSTABLE OPERATION AND CAUSE THE CENTRIFUGE TO MOVE

OFF OF THE OPERATING SURFACE.

DO NOT OPERATE THE CENTRIFUGE IN ATMOSPHERES CONTAIN-

ING FLAMMABLE OR VOLATILE GASES. DO NOT USE THE CENTRI-

FUGE TO SPIN DOWN FLAMMABLE OR VOLATILE LIQUIDS.

4.1 CA6000 Centrifuge Setup for Operation

Turn the On/Off Switch to the On (|) position.

The CA6000 will be in the same operative mode as the last time it was used.

Open the lid by pulling the Lid Release Latch (located on the upper right side of the centrifuge) toward the
front of the centrifuge.

MA0128 16

CA6000 Centrifuge Users Manual

4.2 Loading Balance

All rotating equipment is dynamically balanced at the source. Reference numbers and marks indicate the
exact respective positions of the various parts forming the equipment; when rotating, this should always
be whole and complete.

All buckets must be always inserted into the rotating fixed-angle equipment, even for partial loads.

WARNING

NEVER ATTEMPT TO BALANCE THE LOAD BY ADDING WEIGHTS,

MERCURY, OR METAL SHOT TO THE BOTTOMS OF TUBES OR

SHIELDS. ONLY THE PROPER BUCKETS, INSERTS, AND TUBES

SHOULD BE PLACED IN THE CENTRIFUGE.

Distribute the load correctly. Loads should be inserted two by two, of equal weight, at 180° from the rotation
axis and at equal distance (radius) from the center of rotation. Use matching inserts when balancing the rotor.
When spinning an odd number of samples, even the load by inserting a balance tube filled with water.

For partial loads of swing-out rotating equipment, it is advisable to distribute the tubes symmetrically also
with regard to the center of rotation.

Correct
Sample
Placement
in Insert

Incorrect
Sample
Placement
in Insert

Figure 5  Loading Balance

Avoid overloading the rotating equipment. To centrifuge liquids with density higher than 1200 kg/m3, the
maximum rpm should be reduced. Calculate the new allowable rpm using the following formula:

New allowed rpm = max rpm * 1200

√

New Density

Lower the lid, then close it by pressing on the front edges until the hook in the lid clicks into the lid lock
mechanism. Only this operation will activate the centrifuge starting device.

When using the aerodynamic rotating heads, all tubes must be balanced with ± 2 grams, in order to obtain
the best separation.

MA0128 17

CA6000 Centrifuge Users Manual

4.3 Rotating Equipment Performance

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Performance Table

Type of equipment Max. Speed (rpm) Max. RCF (xg)

42601001 4000 2933

42601026 6500 5289

RCF Calculation

The main factors of the centrifuge action in terms of correct analysis methodology are: the centrifugation
time and the gravity number xg (relative centrifugal force) to which the product is submitted. The formula
allows the determination, as a function of the radius r, of the relative centrifugal force value, expressed in
multiples of the gravity acceleration value g (9.9 m/sec

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2

) for each rpm value between 150 and 20,000.

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The relative centrifugal force and the RPM number are connected by the following relations:

RPM in thousands =

RCF

√

where RCF = relative centrifugal force specified for the particular centrifugation procedure

r = Tip radius of tube (in inches) obtained from the chart above

28.4r

4.4 Selecting Operating Modes

Instructions for setting the following parameters are provided in this section:

H Rpm centrifugation (300 rpm up to 6500 rpm)

H Centrifugation time (1 minute up to 99 minutes, plus Hold)

H Acceleration (Profiles 1-5)

H Deceleration (0-4 Coast-down; 0 inertial)

MA0128 18

CA6000 Centrifuge Users Manual

Setting Acceleration

To select an acceleration profile, use the following procedure:

1 Press and hold the SOFT ACCEL key for approximately 3 seconds, until the rpm display shows

ACL flashing and a number between 1 and 5.

SOFT

ACCEL

2 Use the qp keys to select a profile. 1 is slowest.

High acceleration (LED off)
Low acceleration (LED on)

Setting Deceleration

It is possible to select various low coast-down deceleration curves, corresponding to several profiles
of decreasing speed.

1 Press and hold the SOFT BRAKE key for approximately 3 seconds, until the rpm display shows

dCL flashing and a number between 0 and 4.

SOFT

BRAKE

2 Use the qp keys to select a profile. 0 is coast mode (no braking).

High deceleration (LED off)
Low deceleration (LED on)

Setting Rpm, Time (MIN) Profiles

To set rpm or cycle time, press and hold the q or p key located under the respective parameter.
Release the key when the desired value is reached. The display will flash for 3 seconds after the
adjustment key is released and then stop, indicating that the new parameter is accepted.

How to Set and Memorize a Program

To store the parameters that appear on the display, press a PROGRAM key from 1 to 5 while the
parameter is flashing (the display flashes when setting rpm, minutes, soft acceleration, or decelera­tion). When the LED next to the program key is lit, the program has been memorized.

It is not possible to set a program with unspecified Hold (Hd) time: the timer display will continue
flashing; you must insert a determined time profile.

To restore manual mode, press again the selected program key; the LED goes off and you can make
new parameter selections.

How to Protect a Program

1 To protect a program from overwriting, when you memorize a new program (pressing a PROGRAM

key during flashing phase) you must press simultaneously STOP/PROGRAM LOCK key.

2 To cancel the program protection, press the PROGRAM key together with the STOP/PROGRAM

LOCK key.

MA0128 19

CA6000 Centrifuge Users Manual

4.5 Starting the Centrifuge

1 Make sure the lid is closed.

2 Press the START key.

3 The Centrifuge accelerates to the set speed.

The yellow NOT IN USE LED goes off, and the IN USE LED lights.

The green SOFT ACCEL LED starts flashing. This LED stays on when the set speed equals the
actual speed. (Note that if the set rpm is higher than the maximum speed of the selected rotor (see
Section4.3), the LED continues flashing.)

4 During the run time all displays show real parameters: Rpm, Time (min  elapsed run time in

minutes counts down or remaining run time counts up if you have set a time of Hold). If you are
running a Program, you can show set values by pressing the corresponding arrow key.

If your parameters have been set manually it is possible to change them. To change the rpm or time,
use pq. To change the deceleration mode, press and hold the SOFT BRAKE key for about 3
seconds. The Readouts start to flash and show the set values for 3 seconds. While the Readout
flashes it is possible to change set values.

5 When the run time is completely elapsed, deceleration will take place.

4.6 Stopping the Centrifuge

Once the preset time has elapsed, the centrifuge automatically stops with the preset deceleration mode.

However, in any situation you can press the STOP key to stop the centrifuge.

Note that the cycle will be interrupted automatically for load imbalance, power failure, system error, or
breakdown.

If this occurs, the relevant information appears on the message display.

4.7 Emergency Lid Unlock

In the event of a power failure, the lid can be opened using the special tools supplied with the centrifuge.

This operation should be performed with the power disconnected.

1 Unscrew the nylon screw in the hole on the right side of the centrifuge, just in front of the Lid Release

Latch.

2 Insert the unlocking tool supplied with the centrifuge in the hole.

3 Pivot the unlocking tool downward, and at the same time pull the Lid Release Latch forward.

4 Reinstall the nylon screw

WARNING

SHOULD A POWER FAILURE OCCUR DURING CENTRIFUGATION,

WAIT FOR TEN MINUTES. IF THE ROTOR IS STILL SPINNING,

CLOSE THE LID AND WAIT ANOTHER TEN MINUTES BEFORE RE-

PEATING THE OPERATION.

MA0128 20

Maintenance



WARNING

DURING MAINTENANCE OPERATIONS, ALWAYS DISCONNECT THE

CENTRIFUGE FROM THE POWER SOURCE. OTHER THAN ROTOR

AND EXTERNAL FUSE REPLACEMENT, REFER SERVICING TO

QUALIFIED PERSONNEL.

5.1 Disassembly of the Rotating Head

Remove the head nut securing the rotor to the motor shaft. Lift the rotor straight up.

5.2 Rotating Equipment and Centrifugation Chamber Cleaning

Regular cleaning of the rotating heads and centrifugation chamber is vital for correct operation of the
centrifuge.

At a minumum the unit should be cleaned weekly in warm water containing a few drops of mild nonalkaline
detergent. The centrifuge should always be cleaned immediately after a spillage has occurred. Do not forget
to wash the core of the rotating head which comes into contact with the drive spindle. Each pocket of the
rotating heads, slots, and buckets must be washed thoroughly using a small nylon brush.

MA0128 21

CA6000 Centrifuge Users Manual

CAUTION

Do not use metal wire brushes to clean the centrifuge.

Once the rotating equipment is clean, rinse it in running water, preferably distilled. Dry the rotating
equipment with a soft absorbent non-woven cloth or tissue. Drying may be finished off with warm air jet (e.g.,
a hair dryer).

MAKE CERTAIN THAT ROTATING HEADS, SLOTS, AND BUCKETS ARE WELL DRIED.

NEVER LEAVE damp rotating equipment on a metal surface, particularly stainless steel. An electrochemi­cal reaction could take place with the aluminum or magnesium in the rotor.

For swing-out rotating equipment, be sure to clean the dirty grease from the studs and replace it with a small
amount of fresh grease. This will ensure that the buckets swing freely. The vast majority of apparent
imbalance problems arise from the failure of the user to clean and grease the studs, not from instrument
error.

Never apply grease if studs and balancing slots have not been carefully cleaned. Use only the same kind
of grease furnished in the rotor head packaging.

Once the rotating head has been removed, it is easy to clean the centrifugation chamber (do not use
corrosive solvents or detergents).

5.3 Exterior Cleaning

For a correct cleaning a light domestic use detergent is recommended. A mixture of 50% isopropanol and
water is suggested because it will not damage the labels.

5.4 Disinfection

Autoclave rotating equipment for 20 minutes at 120° C to destroy microorganisms.

Rotor lids must be disassembled from rotor bodies. O rings, autoclaved separately, should be replaced
when deformed.

Any part which has been subjected to temperatures above 130° C must be discarded.

Household bleach used at 10% concentration with 10 minutes immersion is effective against bacteria,
spores, and viruses but, as an oxidizing agent, is corrosive to metal alloys and must be thoroughly rinsed
off and dried. It should never be used if there is surface damage to the rotating equipment.

Note that the black color on the surface or the rotating equipment will be gradually washed out if the rotor
is regularly autoclaved or bleached. This does not necessarily denote a degradation on the anodization.

If there is a biological liquid overflow there may be the possibility of a discharged into the drain hose. Do
not forget to proceed to the decontamination with the same household bleach (10%).

For any other unscheduled cleaning, the customer must contact Becton Dickinson to verify that the
proposed method does not damage the centrifuge.

NOTE

Becton Dickinson makes no claims as to the effectiveness of proprietary

brands or decontaminating solutions.

MA0128 22

CA6000 Centrifuge Users Manual

5.5 Radioactive Decontamination

We recommend that all radioactive decontamination be referred to your Radioactivity Safety Officer.

After decontamination, the unit should be cleaned as described above (Section 5.4).

5.6 Fuses

There are two fuses in the power entry module. The fuses are located in a drawer just below the power cord
plug.

These fuses are:

Diameter 5 x 20 mm F6A/250V

WARNING

BEFORE BEGINNING TO CHANGE THE FUSES, MAKE SURE THE

UNITS POWER IS TURNED OFF AND DISCONNECT THE CENTRI-

FUGE POWER CORD FROM THE POWER SOURCE.

In case of fuse blowing, do not replace them until the machine has been inspected by a maintenance
engineer.

MA0128 23

Troubleshooting



6.1 Alarm Messages

Below is a list of error messages and their meaning.

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MA0128 24

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.noitarepotxenehttasraeppasidegassemehT.sraeppaegassemehtsnruter

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notceBtcatnoc,sedocXXErehtollaroF.dilehtgninepoerofebpotsotdaehrotor

6.2 Other Problems

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CA6000 Centrifuge Users Manual

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nwolbsiesufeniL)6.5noitceS(esufecalpeR

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Becton Dickinson Instrument Repair: 18005447434

straplanretni

6.3 Repair

Please refer all repairs to a qualified Biomedical Engineer.

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riapeRtnemurtsnInosnikciDnotceBllaC

MA0128 25

Limited Warranty

f

Becton Dickinson warrants the Clay Adams CA6000 Centrifuge to be free from defects in workmanship and
materials for a period of 18 months from date of installation, provided the Centrifuge is operated in
accordance with this Users Manual. During such period, Becton Dickinson agrees to ship replacement
parts at no additional costs to the user which, in Becton Dickinsons sole judgment, are found to be
defective, provided the Centrifuge has not been subjected to misuse or abuse. The warranty stated herein
shall extend to the original consumer only and not to any subsequent consumer of the Centrifuge.

Becton Dickinson shall not be liable for any incidental or consequential damages. Becton Dickinson makes
no other warranties, expressed or implied, except as stated herein.

MA0128 26

Parts and Accessories

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MA0128 27

CA6000 Centrifuge Users Manual

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MA0128 28

International Contacts

h

Becton Dickinson de
Mexico, S.A. de C.V.
Monte Pelvoux 111  9th Floor
Col. Lomas de Chapultepec
11000 Mexico D.F.
Voice: 52 5 237 1200  Fax: [phone]

Becton Dickinson
Asia Pacific Division
30 Tuas Avenue 2
SIngapore 639461
Voice: (65) 8610633  Fax: [phone]

Nippon Becton Dickinson Co., Ltd.
Akasaka DS Building
526 Akasaka 8-chome
Minato-ku, Tokyo 107
Japan
Voice: (81) 3 54138181  Fax [phone]

Becton Dickinson
Latin America Diagnostics Division
Rua Alexandre Dumas 1976
04717-004 Sao Paulo, S.P. Brazil
Voice: (55) 11 5459833  Fax: [phone]

Becton Dickinson
Becton Dickinson and Company
2464 South Sheridan Way
Mississauga, Ontario
Canada L5J 2M8
Voice: [phone]  Fax: [phone]

Becton Dickinson
European Divisions
5 Chemin des Sources  BP 37
38241 Meylan CEDEX France
Voice: 33 4 76 416464  Fax: [phone]

Becton Dickinson
Microbiology Systems
7 Loveton Circle
Sparks, Maryland 21152
USA
Voice: [phone]  Fax: [phone]
Toll-Free: Technical Services: 1-800-631-8064

Instrument Repair: 1-800-544-7434

MA0128 29

Exploded View Drawing

i

MA0128 30

CA6000 Centrifuge Users Manual

MA0128 31