Beckman Coulter SX4750A Instruction For Use, Installation And Assembly

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Instructions For Use

Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.

SX4750A Swinging-Bucket Rotor

For Use in the Beckman Coulter Allegra X-12 Series, Allegra X-14 Series, and Allegra X-15R Centrifuges

GX-TB-004EE February 2018

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SX4750A Swinging-Bucket Rotor

Beckman Coulter Eurocenter S.A. 22, rue Juste-Olivier Case Postale 1044 CH - 1260 Nyon 1, Switzerland Tel: +41 (0) 22 365 36 11

GX-TB-004EE (February 2018)

Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

If you have any questions, contact our Customer Support Center.

• Worldwide, find us via our website at

www.beckmancoulter.com/customersupport/support

• In the USA and Canada, call us at 1-800-369-0333.

• Outside of the USA and Canada, contact your local

Beckman Coulter Representative.

Original Instructions

Page 3

Revision History

When a subsequent version changes the information in this document, a new issue will be released to the Beckman Coulter website. For updates, go to beckmancoulter.com/ifu and download the most recent manual or system help for your instrument.

Issue EB, 8/13

The following section has changed subsequent to revision EA:

• Replacement Rotor Parts

Issue EC, 8/13

The following sections have changed subsequent to revision EB:

• Table 2, Beckman Coulter Tubes and Bottles for the SX4750A Rotor

• Beckman Coulter, Inc. SX4750A Warranty

Issue ED, 11/15

The following sections have changed subsequent to revision EC:

• Symmetrical and Balanced Loading

Issue EE, 02/18

The following sections have changed subsequent to revision ED:

• Description, footnote * on page -2

• Aerosolve Cannisters, footnote * on page -4

• Using Multiwell-Plate Carriers, step 3 on page -16

• Replacement Rotor Parts

Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin of the amended page.

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Revision History

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Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to use this equipment. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.

This safety notice summarizes information basic to the safe use of the rotor described in this manual. The international symbol displayed to the left is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equipment is attempted. When you see the symbol on other pages of this publication, pay special attention to the safety information presented. Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor.

Alerts for Warning, Caution, and Note

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

Safety Notice

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

NOTE

NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

Safety Information for the SX4750A Rotor

Handle body fluids with care because they can transmit disease. No known test offers complete assurance that such fluids are free of micro-organisms. Some of the most virulent— Hepatitis (B and C) viruses, HIV (I–V), atypical mycobacteria, and certain systemic fungi—further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment.

Do not run toxic, pathogenic, or radioactive materials in this rotor without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

This rotor was developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a

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Safety Notice

Safety Information for the SX4750A Rotor

centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter’s approval.

Although rotor components and accessories made by other manufacturers may fit in the SX4750A rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the SX4750A rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the components and accessories listed in this publication should be used in this rotor.

Attach all four buckets or carriers, loaded or empty, to the rotor for every run. Make sure that filled containers are loaded symmetrically into the rotor and that opposing containers are filled to the same level with liquid of the same density.

The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials in nor handle or store them near the ultracentrifuge.

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section on

Run Speeds.

Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins in scratches and may open fissures in the rotor with continued use.

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Revision History,iii

Safety Notice,v

Alerts for Warning, Caution, and Note,v

Safety Information for the SX4750A Rotor,v

SX4750A Swinging-Bucket Rotor,1

Specifications,1

Description,2

Buckets and Accessories,3

Modular Disk Adapters,3 Bottle Adapters,3 Blood Bag Cups,3 Aerosolve Cannisters,4 Cell Culture Flask Adapters,4 Multiwell-Plate Carriages,5

Contents

Run Preparation,5

Prerun Safety Checks,5 Installing the Rotor Yoke,6

Loading the Buckets,7

Symmetrical and Balanced Loading,7 Using Tube and Bottle Buckets,8

Using Tubes,10 Assembling Modular Disk Adapters,10 Loading Tubes in Adapters,11 Loading Adapters in the Buckets,11 Using Bottles in Adapters,11 Using Aerosolve Cannisters,12 Using Blood Bag Cups,12 Using Cell Culture Flask Adapters,14 Using Bucket Covers,14 Loading Buckets Onto the Yoke,15

Using Multiwell-Plate Carriers,15

Operation,16

Removal and Sample Recovery,17 Tubes, Bottles, and Accessories,17

Temperature Limits,17 Labware,17

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Contents

Run Speeds,24

Care and Maintenance,25

Maintenance,25 Cleaning,26

Modular Disk Adapters,27 Bottle Adapters and Blood Bag Cups,27

Cell Culture Flask Adapters,27 Decontamination,27 Sterilization and Disinfection,28 Tube Breakage,28

Aerosolve Cannisters,29

SST or Corvac Tubes,29 Storage,30

Returning a Rotor,30

Supply List,31

Replacement Rotor Parts,31 Other,31

Beckman Coulter, Inc. SX4750A Warranty

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Illustrations

1 Examples of Correctly and Incorrectly Loaded Buckets and

Carriers,9 2 Assembling a Modular Disk Adapter,10 3 Blood Bag Loading Procedures,13 4 Lubricating Pivot Pin/Bucket Contact Areas,26

Illustrations

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Tables

1 Adapters and Blood Bag Cups Available for the SX4750A

Rotor,18 2 Beckman Coulter Tubes and Bottles for the SX4750A Rotor,20 3 Beckman Coulter Labware for Use with Multiwell-Plate

Carriers,23 4 Commercially Available Labware for Use with Multiwell-Plate

Carriers,24

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SX4750A

min

max

min

max

Swinging-Bucket Rotor

Specifications

Maximum speed

Allegra X-15R (tube-and-bottle buckets) .............................. 4750 RPM

Allegra X-15R (multiwell-plate carriers)............................... 4450 RPM

Allegra X-14 series (tube-and-bottle buckets) ..................... 4300 RPM

Allegra X-14 series (multiwell-plate carriers)...................... 4000 RPM

Allegra X-12 series (all buckets) ............................................. 3750 RPM

Critical speed range

Maximum solution density .......................................................... 1.2 g/mL

Relative Centrifugal Field

using tube-and-bottle buckets (r using multiwell-plate carriers (r

Conditions requiring speed reductions .................... see Speed Derating

Number of buckets/carriers ........................................................................4

Available tubes and bottles........................................................ see Table 2

Maximum load allowed in each tube-and

bottle bucket at rated speed

(excluding weight of bucket and cover)............................ 1000 grams

Maximum load allowed in each multiwell-plate

carrier at rated speed (excluding weight

of carrier and cover)............................................................... 360 grams

Total maximum allowable imbalance of opposing loads..........50 grams

Maximum rotor capacity................................................................... 3 liters

1. Axis of Rotation

a. The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing through or running

at the critical speed range is characterized by some vibration.

b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (r

gravity (g) according to the following formula: RCF = r/g — where r is the radius in millimeters,  is the angular velocity in radians per second (2  RPM /60), and g is the standard acceleration of gravity (9807 mm/s

c. RCF inside buckets is 5095 ´ g (tube-and-bottle buckets—r

Approximate acceleration time ............................................... 80 seconds

Approximate deceleration time................................................ 81 seconds

Weight of fully loaded rotor (buckets with covers)....... 15.4 kg (33.9 lb)

Rotor yoke material ...............................................................stainless steel

Bucket and carrier material....................................... anodized aluminum

201.6 mm) or 3919 ´ g (multiwell plate carriers—r

max

............................................................580 to 720 RPM

at maximum speed

= 207.8 mm) ................ 5250  g

max

= 183.2 mm)................. 4060  g

max



2) to the standard acceleration of

). After substitution: RCF = 1.12r (RPM/1000)

176.7 mm).

max

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SX4750A Swinging-Bucket Rotor

Description

1. Yoke

2. Bucket

3. Bottle

4. Carrier

5. Carriage

This Beckman Coulter rotor has been manufactured in an ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges.

The SX4750A is a four-place swinging bucket rotor. The rotor buckets carry adapters that allow centrifugation of a wide range o f tube and bottle sizes (from 1.5 mL reaction vials to 750-mL bottles) or single- to quad-pack blood bags as well as multiwell plates in carriers. Special adapters are also

available that allow centrifugation of Corning canted-neck 75-cm

and 25-cm2 cell culture flasks. The rotor develops centrifugal forces that are suitable for rapidly sedimenting protein precipitates, large particles, cells, and cell debris.

The rotor yoke is made of stainless steel. Black-anodized aluminum buckets and carriers can be run by placing them over pivot pins on the arms of the yoke; they swing out to horizontal position

during centrifugation. Transparent covers made of high-impact plastic have been tested

to demonstrate containment of microbiological aerosols under normal conditions of the associated Beckman Coulter centrifuge when used and maintained as instructed. A captive tie-down device is used to secure the rotor to the drive shaft during centrifugation.

The ARIES (Automated Rotor Imbalance Equilibrating System) technology provides imbalance compensation for rotors with buckets that are up to 50 grams unbalanced.

The centrifuge identifies the rotor during the run by means of a magnetic sensor system in the centrifuge rotor chamber and magnets imbedded in the rotor. The overspeed system ensures that the rotor does not exceed its permitted speed.

Refer to the Warranty at the back of this manual for warranty information.

* Validation of microbiological containment was done at an independent third-party testing facility (Public Health

England). Improper use or maintenance may affect seal integrity and thus containment.

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Buckets and Accessories

Several types of labware can be run in the buckets, depending on your application.

Modular Disk Adapters

Tubes are supported in modular disk adapters, which can also serve as tube racks in the laboratory. The adapter disks are color coded by the tube size they accommodate (see Table 1); the number of disks used in an adapter assembly depends upon the length of tubes used. A tube decanter is available to hold either 10-mm or 12-mm tubes securely in the blue adapter, allowing all the tubes to be decanted at once. Additionally, 1.5-mL Microfuge tubes can be run using a special plate that fits on top of the blue adapter. Both of these accessories are described in Table 1. Beckman Coulter tubes and bottles available for use in the rotor buckets are described in Table 2.

SX4750A Swinging-Bucket Rotor

Description

Bottle Adapters

1. Bottle

2. Adapter

Bottles are supported in polypropylene adapters that fit inside the rotor buckets. The adapters accommodate four bottle sizes, including two conical bottles (see Table 1).

Blood Bag Cups

Polypropylene cups provide support for blood bags in the rotor buckets. Blood bag cups are available in two sizes: one for single- or double-pack bags, and one for triple- or quad-pack bags (see Table 1).

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SX4750A Swinging-Bucket Rotor

Description

Aerosolve Cannisters

1. Lid

2. O-Ring

3. Aerosolve Cannister

Aerosolve cannisters, designed to contain aerosol leakage and liquid spills, can be used in the SX4750A buckets when this additional containment is required. The cannister was tested

to demonstrate containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge, when used and maintained as instructed. Aerosolve cannisters hold a variety of tube sizes in racks, or they can be used as 500-mL wide-mouth bottles.

Cell Culture Flask Adapters

75-cm2 Flask Adapter

Specially designed EPDM (ethylene propylene) adapters can be used to centrifuge Corning 25-cm2 (two per bucket) or 75-cm

the separation and concentration step of the cell culturing process can be accomplished in the flask. Eliminating the need to transfer cultures into tubes for centrifugation and back into flasks minimizes the risk of contamination, as well as saving time and labware.

* Validation of microbiological containment was done at an independent third-party testing facility (Public Health

England). Improper use or maintenance may affect seal integrity and thus containment.

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(one per bucket) canted-neck cell culture flasks. Using these adapters,

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Multiwell-Plate Carriages

Used in the multiwell-plate carriers to facilitate loading and unloading and to provide support to the labware during centrifugation, each multiwell-plate carriage can hold up to four stacked multiwell plates (not to exceed 55.9 mm/2.2 in. in depth) or one deep-well plate. Each carrier can also carry a 96-well kit for high-throughput processing (such as DNA or RNA kit). A polypropylene pad provides support for the labware.

Run Preparation

SX4750A Swinging-Bucket Rotor

Run Preparation

Specific information about the SX4750A rotor is given here. Information about the use and care of the centrifuge is contained in the centrifuge manual, which should be used together with this manual for complete rotor and centrifuge operation.

NOTE

Prerun Safety Checks

IMPORTANT

Make sure that the rotor yoke, buckets and/or carriers, and covers are clean and show no signs of corrosion or cracking.

• If any evidence of damage is present, do not centrifuge the rotor.

Check the chemical compatibilities of all materials used (refer to Chemical Resistances, publication IN-175).

Read the Safety Notice section at the front of this manual before using the rotor.

Verify that the tubes, bottles, and accessories being used are listed in Table 1, Table 2, and

Table 3.

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SX4750A Swinging-Bucket Rotor

Run Preparation

Installing the Rotor Yoke

NOTE

Before the first use of a new rotor, attach the tie-down T-bar (369718) to the rotor yoke. Screw it down

as far as possible.

Before installing the yoke in the centrifuge, lightly lubricate the drive hole with a lubricant such as Spinkote (306812).

CAUTION

Never drop the rotor yoke onto the centrifuge drive shaft. The drive shaft can be damaged if the rotor is forced sideways or dropped onto it.

Carefully lower the yoke straight down onto the centrifuge drive shaft.

• Be sure the yoke is properly seated on the shaft.

Turn the tie-down T-bar to the right (clockwise) to tighten the yoke firmly on the drive shaft.

NOTE

If the rotor yoke is left in the centrifuge between runs, before each run make sure it is properly

seated on the drive shaft, and that the T-bar is tight.

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Loading the Buckets

For runs at other than room temperature, refrigerate or warm the rotor and precool the centrifuge beforehand for fast equilibration.

WARNING

Handle body fluids with care because they can transmit disease. No known test offers complete assurance that such fluids are free of micro-organisms. Some of the most virulent—Hepatitis (B and C) viruses, HIV (I–V), atypical mycobacteria, and certain systemic fungi— further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment.

Do not run toxic, pathogenic, or other hazardous materials in this rotor without taking all appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

SX4750A Swinging-Bucket Rotor

Loading the Buckets

Symmetrical and Balanced Loading

Beckman Coulter supplies buckets and carriers in weight-matched sets to make balancing easier (the weight and retirement date are marked on the side of each bucket or carrier). Modular disk adapters are also sold in weight-matched sets. However, there are variances in weight between sets, as well as variance in weight between previously purchased adapters. To prevent accidental imbalance it is important to keep matched sets of buckets, as well as matched sets of adapters, together and to weigh other adapters to be sure they are approximately the same. Marking matched adapter sets will help you keep them together.

NOTE

To ensure optimal performance and stability, the rotor must be loaded symmetrically. Two factors affect symmetric loading:

1. The buckets or carriers must be loaded symmetrically with respect to their pivotal axes

2. The rotor should be loaded symmetrically with respect to its center of rotation. However,

For best results, load opposing buckets or carriers with the same type of labware. Unlike other rotors, the ARIES rotors do not require careful balancing of opposing loads. Imbalances of up to 50 grams can be accommodated. Do not exceed the rated maximum load for buckets (1000 grams each for tube-and-bottle buckets; 360 grams each for multiwell-plate carriers).

The light purple 500-mL adapters (349945) replace the previous tan adapters. There is a weight difference between the old tan adapters and the current purple adapters. If you run a mix of the purple and tan adapters, be sure to run matching colors in opposing positions or rotor imbalance could occur.

(seeFigure 1).

unlike other rotors, it is not necessary to balance opposing loads carefully.

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SX4750A Swinging-Bucket Rotor

Loading the Buckets

It is not necessary to completely fill all tubes* or positions in buckets; however, partially filled buckets must be balanced with respect to the bucket pivotal axis (see Figure 1). In multitube adapters, each tube should be placed so that its weight is balanced by a tube in a diametrically opposite position across the pivotal axis in the same adapter. Adapters placed in opposing buckets should also be filled the same way.

1. At Rest 2. At Speed

In multiwell plate carriers, samples should be loaded into the wells symmetrically with respect to the pivotal axis of the bucket, and opposing carriers should contain similar loads.

NOTE

The ARIES rotors are designed to accommodate imbalances of up to 50 grams. It is not necessary to carefully balance opposing tubes as in regular rotors. In fact, doing so may result in vibration during early acceleration as the ARIES device adjusts to equilibrium.

Using Tube and Bottle Buckets

Whether you are running tubes, bottles, cell culture flasks, or blood bags, you must load the buckets symmetrically around the center of rotation and each bucket must be loaded symmetrically with respect to its pivotal axis (see Symmetrical and Balanced Loading, above).

* Fill tubes at least half full. Speed reduction may be required for fill volumes below 75 percent.

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Figure 1 Examples of Correctly and Incorrectly Loaded Buckets and Carriers

CORRECT

INCORRECT

Balanced Load Unbalanced Load

SX4750A Swinging-Bucket Rotor

Loading the Buckets

1. Balanced Load

2. Unbalanced Load

3. Pivotal Axis of Bucket

4. Center of Rotation

GX-TB-004EE

5. CORRECT

6. Examples of Symmetrically Loaded Multiwell Plates (load opposite plates the same way)

7. INCORRECT

8. Examples of Nonsymetrically Loaded Multiwell Plates

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SX4750A Swinging-Bucket Rotor

Loading the Buckets

CAUTION

Buckets are designed to be used only with modular disk adapters, bottle adapters, cell culture flask adapters, Aerosolve cannisters, and blood bag cups. Do not pour samples directly into buckets, bottle sleeves, or blood bag cups. Do not load bottles, cell culture flasks, or tubes directly into the buckets. Do not use labware that is not specified for use in this rotor.

You can load buckets before or after they are installed on the rotor yoke. In either case, we recommend filling the appropriate labware first and then loading the labware into the buckets.

Using Tubes

Assembling Modular Disk Adapters

Refer to Table 1 to determine which color adapter is required for the tubes you are using and the number of disks needed to support the length of the tubes.

Select the appropriate adapter base (from Table 1) and attach a bail to it (see Figure 2).

Figure 2 Assembling a Modular Disk Adapter

1. Bails 2. Disks 3. Base

Position one of the disks so that its grooves are aligned with the bail.

• Push the disk down until the bail snaps into the grooves.

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SX4750A Swinging-Bucket Rotor

Loading the Buckets

Add more disks in this way until the height of the assembly is nearly as tall as the tubes you will be using.

• (If the height of the disks is very tall, you may have to push the bail into the grooves of the top disks by hand.)

• Remove or add disks to the bail to accommodate shorter or longer tubes.

• If the tubes fit too snugly in the adapter’s rubber base, apply a light film of powder, such as

talcum powder, to prevent the tubes from sticking.

Loading Tubes in Adapters

When placing tubes in modular disk adapters, it is important to make sure that they will not contact the rotor yoke during a run.

• One way to do this is to place empty tubes in an adapter, place the adapter in a bucket on the rotor yoke, and manually swing the bucket to the horizontal position, making sure that all tubes clear the yoke.

• Another method is to place the adapter in a bucket and position a bucket cover over the tubes, checking that tubes do not touch the cover.

If all positions in an adapter are not filled, load the adapter symmetrically with respect to its pivotal axis (see Symmetrical and Balanced Loading, above).

Loading Adapters in the Buckets

After placing tubes in the disk adapters, lower the adapters into the buckets so that the bails line up with the rotor pivot pins. If only two loaded adapters are run, place them in opposing buckets. The other two buckets should contain empty modular disk adapters (do not centrifuge empty cell culture flask adapters) to prevent imbalance. (See Symmetrical and Balanced Loading, above.

Using Bottles in Adapters

Load bottles into their appropriate adapters (see Table 1), then place the filled adapters in the rotor buckets. If only two bottles are run, place them in opposite buckets. Make sure the other two buckets contain at least a minimal “blank” load (such as empty modular disk adapters) to prevent rotor imbalance. If modular disk adapters are not available, use two water-filled bottles in adapters to balance the load. (See Symmetrical and Balanced Loading, above.)

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SX4750A Swinging-Bucket Rotor

Loading the Buckets

Using Aerosolve Cannisters

CAUTION

Do not run chloroformed samples in Aerosolve cannisters. Chloroform vapors can damage the cannister material.

1. Aerosolve Tube Rack

Aerosolve cannisters can be used as wide-mouth bottles or with tubes in racks that are specially designed to fit in the cannisters. Table 1 lists the Aerosolve tube racks and the number and sizes of tubes they accommodate. When Aerosolve cannisters are used in the SX4750A rotor buckets, pads (361269) must be placed beneath the cannisters in the buckets to provide support and prevent the cannisters from being damaged during centrifugation. These pads are included in the Aerosolve cannister kits (359232). Bucket covers cannot be used with Aerosolve cannisters.

Using Blood Bag Cups

NOTE

The yellow 90-mm cup (356856) accommodates up to two single bags or one double pack; the orange 97-mm cup (356857) supports either one triple or one quad pack. Do not pour liquid directly into blood bag cups. Fit blood bags into cups before loading the cups into the rotor buckets . Load the blood bag cups as follows:

This rotor is suitable for obtaining cell-free plasma or for cell packing.

Load the bags as far down into the cups as possible.

NOTE

Make sure the bags stay as vertical as possible, with no folds at the top or corners. If folds are

present, blood cells could remain in the folds and then mix with the plasma when the bag is removed.

Sandwich the tubing between the blood bag and any transfer packs (see Figure 3).

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Figure 3 Blood Bag Loading Procedures

SX4750A Swinging-Bucket Rotor

Loading the Buckets

1. Blood Bag Label

2. Blood Bag Seam Aligned with

Pivotal Axis of Bucket

3. Ports

4. Blood Bag Seam

5. Blood Bag Cup

6. Sandwich Coiled Tubing between

Transfer Bags and Filled Blood Bag

Make sure the loaded blood bag cups opposite each other on the rotor yoke are approximately the same weight.

• Soft, pliable balancing pads that will not puncture blood bags (see the Supply List) can be

used to maintain weight balance, if necessary.

Place loaded cups into rotor buckets.

• To reduce the possibility of bag breakage, align the blood bag seam with the rotor pivot pins

(see Figure 3) with the label facing out.

Place bucket covers on rotor buckets to prevent blood bags or tubing from contacting the rotor yoke during centrifugation.

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SX4750A Swinging-Bucket Rotor

Loading the Buckets

CAUTION

If bucket covers are not used, make sure the superstructure of the blood bag protruding from the cup does not inhibit the bucket from reaching its horizontal position. If it does, remove the cup from the rotor and reposition the blood bag so that it seats further into the cup. Allowing the blood bags to contact the rotor yoke during centrifugation can cause the bucket to come off the pivot pins and can seriously damage both the rotor and the centrifuge.

If only two filled cups are run, place them in opposing buckets. The other two buckets should contain similar “blank” loads to prevent imbalance (either empty modular disk adapters or waterfilled blood bags in cups).

Using Cell Culture Flask Adapters

Refer to publication GX-TB-007 for 25-cm2 flask adapter information or GX-TB-006 for 75-cm2 flask adapters. (These publications are supplied with the respective adapters.)

NOTE

A dry release agent is used to facilitate removal of cell culture flask adapters from buckets following centrifugation. To avoid compromising the bucket/cover bioseal, the release agent should not be allowed on the bucket O-ring. When running cell culture flasks, use the provided adapter to apply the dry release agent to the bucket cavity and take care to avoid contamination of the O-ring.

Using Bucket Covers

NOTE

Install the cover as follows:

Because of the shape of the covers, 130-mm tubes can be used only in the center cavities of the adapters when covers are used.

Make sure that the bucket and the bucket cover surfaces are clean, dry, and undamaged.

Make sure that the O-ring (961648) is in good condition and lightly coated with silicone vacuum grease (335148).

• Place the O-ring on the ledge inside the bucket.

1. Insert O-ring here

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SX4750A Swinging-Bucket Rotor

Loading the Buckets

Place the cover on the bucket with the latches perpendicular to the pin sockets.

Snap the latches down and secure them under the bucket rim.

Loading Buckets Onto the Yoke

Attach each bucket to the yoke by aligning the grooves in the bucket sides with the pivot pins, then sliding the buckets down until the pivot pins are seated in the bucket pockets.

CAUTION

Attach all four buckets, loaded or empty, to the rotor yoke. If only two buckets are filled, place them in opposite positions on the yoke. All four positions must contain buckets or carriers during a run. Never run the rotor with only two positions filled.

Gently swing the buckets to ensure that they are properly seated on the pivot pins.

Using Multiwell-Plate Carriers

If only two filled multiwell-plate carriers are run, install them opposite each other in the rotor and run two additional carriers or buckets (they can be empty) to prevent rotor imbalance.

Insert the filled plate(s) into the carriage.

• If using two or three Beckman Coulter plates per carrier, place a 96-well cap strip (see

Table 3) between the plates to prevent breakage during centrifugation.

NOTE

Up to three Beckman Coulter plates, separated by cap strips, can be run per carrier at maximum multiwell-plate speeds. If running four Beckman Coulter plates, cap strips cannot be used due to height limitations; reduce run speed to 2700 RPM. Observe manufacturer’s run

speed and stacking limitations if using other manufacturers’ multiwell plates. limitations vary widely between manufacturers—pretest other manufacturers’ plates using water instead of valuable sample.

RCF and stacking

*See Table 4 on page 24 for plates recommended for use in stacks of four at maximum speed.

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SX4750A Swinging-Bucket Rotor

Operation

Grasp the carriage by the handles and lower it into the carrier.

Make sure that carrier cover gaskets are in good condition and secured to the covers.

IMPORTANT

contaminants that could affect the seal.

Put covers on the carriers with the latches perpendicular to the pin sockets.

• Snap the latches down and secure them under the carrier rim.

Attach each carrier to the yoke by aligning the grooves in the sides with the pivot pins, then sliding the carrier down until the pivot pins are seated in the pin pockets.

The seal surfaces of the gaskets and carriers must be free of dust, fibers, and other

CAUTION

All four positions must contain buckets or carriers during a run. Never run the rotor with only two positions filled. If two tube-and-bottle buckets and two multiwell-plate carriers are run, position the similar buckets opposite each other. Select multiwell-plate carrier speed when running mixed sets.

Gently swing the carriers to ensure that they are properly seated on the pivot pins.

Operation

Refer to the instrument instruction manual for centrifuge operation.

NOTE

See Run Speeds for information about speed limitations.

1-16

When using multiwell-plate carriers, enter SX4750A as the rotor and limit top speed of the instrument to 3750 RPM in an Allegra X-12 series centrifuge, 4000 RPM in an Allegra X-14 series centrifuge, or 4450 RPM in an Allegra X-15R centrifuge (when running up to three stacked Beckman Coulter plates separated by cap strips; reduce speed to 2700 RPM if running four stacked plates).

Set rotor speed using RPM values only. To set speed to a specific RCF, use the RPM-to-RCF conversion chart (GX-TB-015) to determine the applicable RPM to enter.

GX-TB-004EE

Page 27

Removal and Sample Recovery

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

Remove the buckets or carriers from the centrifuge.

Remove the labware from the buckets or carriers.

If removing the rotor yoke, turn the tie-down T-bar to the left (counterclockwise) to loosen it.

• Lift the yoke straight up and off the drive shaft.

SX4750A Swinging-Bucket Rotor

Operation

Tubes, Bottles, and Accessories

The rotor uses labware listed in Table 1, Table 2, and Table 3. RCF limitations for some non-Beckman Coulter multiwell plates are shown in Table 4.

Temperature Limits

• Beckman Coulter plastic tubes and bottles have been centrifuge tested for use at temperatures

between 2 and 25°C. For centrifugation at other temperatures, pretest tubes under anticipated run conditions.

• If plastic containers are frozen before use, make sure that they are thawed to at least 2°C prior to centrifugation.

Labware

Beckman Coulter tubes and bottles listed in Table 2 can be run in the SX4750A rotor. Fill tubes at least half full; speed reduction may be required for fill volumes below 75 percent. Refer to Table 3 for Beckman Coulter labware that can be centrifuged in multiwell-plate carriers.

GX-TB-004EE

1-17

Page 28

SX4750A Swinging-Bucket Rotor

Operation

Table 1 Adapters and Blood Bag Cups Available for the SX4750A Rotor

MODULAR DISK ADAPTERS (polypropylene)

Color Code

blue 3

Nom. Tube Vol. (mL)

Nom. Tube Dia. (mm)

10 12

Max. No. Tubes per Adapter

Max. No. Tubes in Rotor

max

Adapter Bottom (mm)

RCF at Maximum Speed ( g)

No. Disks per Adapter

Adapter Part No.

Set of Two

Set of Four

37 148 184.7 4659 5 359469 359148

tan 3 & 5 13 30 120 184.7 4659 5 359478 359157

orange 7 & 10 14 24 96 184.7 4659 6 359470 359149

purple 12 16 19 76 184.7 4659 7 359471 359150

green

15 18 14 56 194.7 4911 6 359472 359151

(conical)

green 15 & 20 18 14 56 184.7 4659 7 359473 359152

lt. green

30 & 50 30 4 16 191 4817 5 359475 359154

(conical)

yellow 50 29 7 28 184.7 4659 6 359474 359153

dk. blue 50 35 4 16 184.7 4659 7 359476 359155

brown 100 44 2 8 184.7 4659 3 359477 359156

tube decanter35

10 12

37 148 — — 1

343108 (each)

—

1.5-mL

1.5 & 1.8 11 26 104 — — 1 adapter plate

a. Tube retainers and adapter plates are sold individually.

SOLID ADAPTER (polypropylene)

Color Code

grey 50-mL

Nominal Tube Volume

Nominal Tube Size

Maximum Number of Tubes per Adapter

Maximum Number of Tubes in Rotor

max

Adapter Bottom (mm)

RCF at Maximum Speed ( g)

29  115 mm 7 28 195.6 4943

conical

a. Do not autoclave this adapter. Use cold/chemical sterilization techniques only. Refer to Sterilization and Disinfection.

354511

—

(each)

Adapter Part No.

Set of Two

393266

Set of Four

393267

1-18

GX-TB-004EE

Page 29

CELL CULTURE FLASK ADAPTERS (EPDM)

SX4750A Swinging-Bucket Rotor

Operation

Color Code

Flask Size (cm2)

Number Flasks per Adapter

at Flask

max

Bottom (mm)

RCF at Max mum Speed ( g)

Part Number (qty two)

orange 75 1 195.3 2080 369292

green 25 2 177.5 2000 369295

BLOOD BAG CUPS (polypropylene)

Color Code Cup Capacity Size

(mm)

yellow single bag

90 2 196.2 4949 356856

Number Bags per Cup

at Cup

max

Bottom (mm)

RCF at Maximum Speed ( g)

Part Number (qty one)

double pack

orange triple pack

97 1 196.7 4961 356857

quad pack

AEROSOLVE CANNISTER RACKS (polypropylene)

Color Code

white 1.5 11 24 96 174 4389 354495

Nom. Tube Vol. (mL)

Nom. Tube Dia. (mm)

Max. No. Tubes per Adapter

Max. No. Tubes in Rotor

max

Adapter Base (mm)

RCF at Maximum

Speed ( g)

Adapter Part Number

Set of Two Set of Four

—

(each)

blue 3 & 5 12 24 96 174 4389 359482 359160

tan 5 12 24 96 180 4530 359489 358993

orange 10 14 18 72 175 4414 359483 359161

purple 12

3 & 5

white

15 14 10 40 174 4389 344517

(vials)

green 15 & 20

3 & 5

lt. green 15

16 12

18 12

12 6

177 178

4469 4490

359484 359162

—

(each)

48 24

174 176

181

4389 4439

4565

359485 359163

359487 358991

(conical)

3 & 5 (round

180

4539

bottom)

lime green

50 (conical)

3 & 5 (round

181

180

2850

4539

359488 358992

bottom)

yellow 50

3 & 5

29 12

4 4

16 16

177 178

4464 4490

359486 359164

GX-TB-004EE

1-19

Page 30

SX4750A Swinging-Bucket Rotor

Operation

AEROSOLVE CANNISTER RACKS (polypropylene)

Color Code

Nom. Tube Vol. (mL)

Nom. Tube Dia. (mm)

Max. No. Tubes per Adapter

Max. No. Tubes in Rotor

max

Adapter Base (mm)

RCF at Maximum

Speed ( g)

Adapter Part Number

Set of Two Set of Four

orange 230 62 1 4 180 4534 — 356985

Cannister

Kit

a. Tube racks used with Aerosolve cannisters do not provide full tube support; some manufacturers’ plastic and glass tubes cannot withstand the

b. Cannisters and lids are made of polyphenylsulphone; O-rings are ethylene propylene. Cannister kit includes the pad (361269) that must be used

500 — — — 183 4616 359481 359232

maximum forces generated by this rotor when used in these racks. Beckman Coulter highly recommends that you pretest other manufacturers’ tubes (in the appropriate Aerosolve cannister labware) using water samples.

beneath the cannister.

BOTTLE ADAPTERS (polypropylene)

Color Code Nominal

Bottle Volume (mL)

orange

230 62 4 195.1 4921 356983 (use

(conical)

Nominal Bottle Diameter (mm)

Maximum Number Bottles in Rotor

max

at Bottle Bottom (mm)

RCF at Maximum Speed ( g)

Adapter Part Number (qty one)

with 349946)

yellow 250 62 4 195.1 4921 349946

warm red

250

62 4 203.2 5125 349849

(conical)

light purple

500 70 4 200.2 6050 349945

blue 750 96 4 195.2 4921 349846

a. Corning polypropylene bottle.

b. Light purple adapter replaces the previous tan adapter. See note under Symmetrical and Balanced Loading for weight difference information.

Table 2 Beckman Coulter Tubes and Bottles for the SX4750A Rotor

OPEN-TOP TUBES

Dimensions Volume

(mL)

16  76 mm 10 polypropylene 355640 349471

16  76 mm 10 polycarbonate 355630 349471

16  76 mm 10 stainless steel 301108 359471

Description Part Number Adapter

Set of Two Set of Four

359484

359150

359162

359150

359162

359150

359162

18  98 mm 15 polycarbonate 342080 359473 359152

18  98 mm 15 polyethylene 342081 359473 359152

1-20

GX-TB-004EE

Page 31

Table 2 Beckman Coulter Tubes and Bottles for the SX4750A Rotor (Continued)

OPEN-TOP TUBES

SX4750A Swinging-Bucket Rotor

Operation

Dimensions Volume

(mL)

Description Part Number Adapter

Set of Two Set of Four

18 98 mm 15 polypropylene 342082 359473 359152

29  104 mm 50 polycarbonate, graduated 363075

(pkg/8)

29  103 mm 50 polypropylene 357007 359474

29  103 mm 50 polycarbonate 363647 359474

359474 359486

359486

359153

359164

359153

359164

359153

359164

TUBES WITH SNAP-ON CAPS

11  38 mm 1.5 polypropylene 357448 359469

354511 354495

11  38 mm 1.5 polypropylene 356090 359469

354511 354495

11  38 mm 1.5 blue polypropylene 356091 359469

354511 354495

359148

11  38 mm 1.5 green polypropylene 356092 359469

354511 354495

11  38 mm 1.5 yellow polypropylene 356093 359469

354511 354495

11  38 mm 1.5 orange polypropylene 356094 359469

354511 354495

11  38 mm 1.5 polypropylene (cap separate) 343169 359469

354511 354495

11  39 mm 1.8 white

polyethylene

340196 359469

354511 354495

29  103 mm 50 polypropylene 357005 359474

359486

29  103 mm 50 polycarbonate 363664 359474

359486

a. Fill tubes at least half full. Speed reduction may be required for fill volumes below 75 percent.

359148

359153

359164

359153

359164

GX-TB-004EE

1-21

Page 32

SX4750A Swinging-Bucket Rotor

Operation

b. Adapter used in Aerosolve cannister (part number 359232).

c. Tube retainers and adapter plates are sold individually.

CONICAL TUBES

Dimensions Volume

(mL)

Description Part Number Adapter

Set of Two Set of Four

62  141 mm 230 polycarbonate (with cap) 356987 349946 356983

356985

62  141 mm 230 polypropylene (with cap) 356989 349946 356983

356985

BIOVIALS

14  55 mm 4 polypropylene 566353 359470

359149 344517 (each)

a. Fill tubes at least half full. Speed reduction may be required for fill volumes below 75 percent.

BOTTLES

Dimensions Volume

(mL)

29 104 mm 50 polycarbonate (with cap assembly) 361693 359474

29  104 mm 50 polycarbonate (with screw cap) 357002 359474

29  104 mm 50 polypropylene (with cap assembly) 357001

29 104 mm 50 polypropylene (with screw cap) 357003 359474

28.5  107 mm 50 Teflon with high-speed screw cap

62  141 mm 230 conical, wide-mouth polycarbonate

Description Part

Number

or 361694

363076

356987

Adapter

Set of Two Set of Four

359153

359486

359164

359153

359486

359474 359486

359164

359153

359164

359153

359486

359474 359486

356983

359164

359153

359164

—

(use w/349946)

62  141 mm 230 conical, wide-mouth polypropylene

356989

356983

—

(use w/349946)

62  136 mm 250 polycarbonate (with screw cap,

355673 349946 —

round bottom)

62  122 mm 250 wide-mouth polycarbonate

356013 349946 —

(with cap)

62  122 mm 250 wide-mouth polypropylene

(with cap)

62  122 mm 250 wide-mouth polycarbonate 358275 349946 —

1-22

356011 349946 —

GX-TB-004EE

Page 33

BOTTLES

SX4750A Swinging-Bucket Rotor

Operation

Dimensions Volume

(mL)

Description Part

Adapter

Number

Set of Two Set of Four

62  120 mm 250 wide-mouth polypropylene 358326 349946 —

69  160 mm 500 polypropylene (with cap assembly)

69  159 mm 500 polypropylene (with cap)

69  159 mm 500 polypropylene

96  130 mm 750

a. Fill tubes at least half full. Speed reduction may be required for fill volumes below 75 percent.

b. Adapter used in Aerosolve cannister (part number 359232).

c. These tubes cannot be run with bucket covers in place.

d. Replacement bottle cap part number is 356263 (set of 6).

e. Do not load the 750-mL bottle directly into the bucket; always use the adapter.

polycarbonate (with screw cap

polypropylene (with screw cap

355607

355665

355650

) 358299

) 356855

349945 —

349846 —

Table 3 Beckman Coulter Labware for Use with Multiwell-Plate Carriers

Description Volume Part Number Available Accessory

Description Part Number

Multiwell plate, 96-well, nonsterile 300 L/well

609844 (pkg/100)

Deep-well polystyrene plate, 96-well, nonsterile

Deep-well polystyrene plate, 96-well, sterile

Deep-well polypropylene plate, 96-well, nonsterile

Deep-well polypropylene plate, 96-well, sterile

1 mL/well 267001

(pkg/24)

1 mL/well 267004

(pkg/24)

1 mL/well 267006

(pkg/24)

1 mL/well 267007

(pkg/24)

Square-well polypropylene plate 2 mL/well 140504

(pkg/24)

Cap strip, nonsterile

Cap strip, sterile

Aluminum foil lid

Cap strip, nonsterile

Cap strip, sterile

Aluminum foil lid

Cap strip, nonsterile

Cap strip, sterile

Aluminum foil lid

Cap strip, nonsterile

Cap strip, sterile

Aluminum foil lid

Cap strip, nonsterile

Cap strip, sterile

Aluminum foil lid

267002 (pkg/10)

267005 (pkg/10)

538619 (pkg/100)

267002 (pkg/10)

267005 (pkg/10)

538619 (pkg/100)

267002 (pkg/10)

267005 (pkg/10)

538619 (pkg/100)

267002 (pkg/10)

267005 (pkg/10)

538619 (pkg/100)

267002 (pkg/10)

267005 (pkg/10)

538619 (pkg/100)

GX-TB-004EE

1-23

Page 34

SX4750A Swinging-Bucket Rotor

reduced maximum speed = (4750 RPM)

1000 grams

heaviest load in grams

-------------------------------------------- -----------

reduced maximum speed = (4450 RPM)

360 grams

heaviest load in grams

-------------------------------------------- -----------

Operation

a. Caps are optional; however, if stacking two or three multiwell plates, use cap strips between plates for speeds greater than 2700 RPM.

b. Requires soft rubber roller (4-in.), part number 538618, for installation.

Table 4 Commercially Available Labware for Use with Multiwell-Plate Carriers

Description Volume Part Number Required Accessory

Description Part Number

BD Falcon

96-well flat-bottom assay

plate, clear polystyrene, standard

300 L/well

BD Falcon 353915 (5/bag, 50/case)

surface, nonsterile

BD Falcon 96-well flat-bottom ELISA plate, clear polystyrene, enhanced

300 L/well

BD Falcon 353279 (25/sleeve, 100/case)

surface, non sterile

BD Falcon 96-well flat-bottom assay plate, clear polystyrene, standard

300 L/well

BD Falcon 353228 (10/bag, 60/case)

surface, nonsterile

a. BD Falcon is a trademark of Becton, Dickinson and Company.

b. These plates can be run at 4450 RPM (4063  g), up to four plates per carrier, without lids or cap strips.

Run Speeds

The centrifugal force at a given radius in a rotor is a function of speed. Comparisons of forces between different rotors are made by comparing the rotors’ relative centrifugal fields (RCF). When rotational speed is adjusted so that identical samples are subjected to the same RCF in two different rotors, the samples are subjected to the same force. The RCF at each speed is automatically calculated by the centrifuge software; if the RCF is entered, the centrifuge calculates the equivalent RPM (revolutions per minute). Run speeds must be reduced in the following circumstances:

• If the weight of the load in a tube-and-bottle bucket exceeds 1000 grams, or if the solution density is more than 1.2 g/mL, reduce the maximum allowable run speed according to the following equation:

——

• If the weight of the load in a multiwell-plate carrier exceeds 360 grams, reduce the maximum allowable run speed according to the following equation:

Do not select rotational speed in excess of:

• 4750 RPM for tube-and-bottle buckets or 4450 RPM for multiwell-plate carriers in an Allegra X-15R,

• 4300 RPM for tube-and-bottle buckets or 4000 RPM for the multiwell-plate carriers in an Allegra X-14 series, or

• 3750 RPM for either buckets or carriers in an Allegra X-12 series centrifuge.

1-24

GX-TB-004EE

Page 35

(Up to three Beckman Coulter plates can be run per carrier at maximum multiwell-plate speeds. If running four Beckman Coulter plates, reduce run speed to 2700 RPM. Observe manufacturer’s run speed and stacking limitations if using other manufacturers’ multiwell plates.) If using multiwell plates for runs exceeding 2 hours in a warm and/or humid environment, speed reduction may be required to maintain low sample temperatures.

Care and Maintenance

Maintenance

SX4750A Swinging-Bucket Rotor

Care and Maintenance

NOTE

• Refer to publication TJ6-TB-011 (supplied with the cannister kit) for information on Aerosolve

• Refer to publication GX-TB-007 for information on 25-cm

Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins

in scratches and may open fissures in the rotor with continued use.

Periodically (at least monthly) inspect the rotor yoke, buckets, and/or multiwell-plate carriers, especially inside cavities, for rough spots or pitting, white powder deposits— frequently

aluminum oxide—or heavy discoloration.

• If any of these signs are evident, do not run the rotor.

• Contact your Beckman Coulter representative for information about the Field Rotor

Inspection Program and the rotor repair center.

Approximately every 400 runs, and after cleaning and/or autoclaving, wipe the rotor pins and pin sockets (see Figure 4) with a paper towel, then coat the sockets with Paint On Graphite

Lubricant (977212).

• Allow the lubricant to dry for at least 5 minutes before installing the rotor in a centrifuge.

cannister maintenance.

cell culture flask adapter

maintenance or GX-TB-006 for 75-cm

flask adapters.

Store the rotor in a dry environment (not in the centrifuge). Refer to Chemical Resistances (publication IN-175) for the chemical compatibilities of rotor and accessory materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center.

GX-TB-004EE

1-25

Page 36

SX4750A Swinging-Bucket Rotor

Care and Maintenance

Figure 4 Lubricating Pivot Pin/Bucket Contact Areas

Cleaning

1. Lubrication Area (both pin sockets) 2. Rotor Pin

Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred. Do not allow corrosive materials to dry on the rotor.

Under normal use, wash the rotor frequently to prevent buildup of residues.

Wash the rotor yoke, buckets, bucket covers, and multiwell-plate carriers and covers in a mild detergent, such as Beckman Solution 555 (339555), that won’t damage the rotor.

• The Rotor Cleaning Kit (339558) contains two plastic- coated brushes and two quarts of

Solution 555 for use with rotors and accessories.

— Dilute the detergent 10 to 1 with water.

NOTE

Thoroughly rinse the cleaned rotor components with distilled water.

Do not wash rotor components in a dishwasher. Do not soak in detergent solution for long periods,

such as overnight.

Air-dry the rotor components upside down.

• Do not use acetone to dry the rotor.

1-26

GX-TB-004EE

Page 37

SX4750A Swinging-Bucket Rotor

Care and Maintenance

Before reinstalling the rotor yoke, lightly lubricate the drive hole with Spinkote (306812) to prevent the rotor from sticking.

Modular Disk Adapters

To disassemble adapters for washing, first pull the bail out of the groove in the disks, then remove the disks and unsnap the bail from the rubber bottom.

Use a mild detergent such as Solution 555 (339555), diluted 10 to 1 with water, and a soft brush to scrub the adapters.

Rinse and dry, then reassemble.

Bottle Adapters and Blood Bag Cups

Use a mild detergent such as Beckman Solution 555 (339555), diluted 10 to 1 with water, and a soft brush to clean bottle adapters and blood bag cups.

Thoroughly rinse with water and air-dry upside down.

Cell Culture Flask Adapters

Refer to publication GX-TB-007 (25-cm2 flask adapters) or GX-TB-006 (75-cm2 flask adapters) for cell culture flask adapter cleaning instructions.

Decontamination

If aluminum rotor components become contaminated with radioactive material, decontaminate them using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum: RadCon Surface Spray or

IsoClean Solution (for soaking),

GX-TB-004EE

and Radiacwash.

†

1-27

Page 38

SX4750A Swinging-Bucket Rotor

Care and Maintenance

While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of decontamination is expressed or implied. Consult your laboratory safety officer regarding the proper decontamination methods to use.

If the rotor or accessories are contaminated with toxic or pathogenic solutions, follow appropriate decontamination procedures. Check Chemical Resistances to be sure the decontamination method will not damage any part of the rotor.

Sterilization and Disinfection

• The rotor yoke, buckets, adapters, and multiwell-plate carriers can be autoclaved at 121°C for

up to an hour. Blood bag cups, bucket and carrier covers, and Aerosolve cannisters can be autoclaved at that temperature for about 15 minutes. (Do not autoclave Aerosolve cannisters purchased prior to April 1993, or any components made of Noryl.) Remove the covers from the buckets and place the rotor yoke, buckets, covers, and/or multiwell-plate carriers in the autoclave upside down. Before autoclaving, remove the air-vent filter from each cover by gently pushing it out from underneath the cover with a pencil or other non-metal tool that will not scratch the cover material. After autoclaving, insert a new air-vent filter (368148) into each cover.

• Ethanol (70%) those made of plastic. Bleach (sodium hypochlorite) may be used, but may cause discoloration of anodized surfaces. Use the minimum immersion time for each solution, per laboratory standards.

or hydrogen peroxide (6%) may be used on all rotor components, including

While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

Refer to publication IN-192, included in each box of tubes or bottles, for tube sterilization and disinfection procedures.

Tube Breakage

If a glass tube breaks, remove the glass very carefully from the adapter and bucket. Imbedded glass particles that remain in the bucket or adapters can cause tube failure during subsequent runs.

* In U.S., contact Nuclear Associates (New York); in Eastern Europe and Commonwealth States, contact Victoreen GmbH

(Munich); in South Pacific, contact Gammasonics Pty. Ltd. (Australia); in Japan, contact Toyo Medic Co. Ltd. (Tokyo).

† In U.S., contact Biodex Medical Systems (Shirley, New York); internationally, contact the U.S. office to find the dealer

closest to you.

* Flammability hazard. Do not use in or near operating ultracentrifuges.

CAUTION

To reduce the potential for corrosion, clean buckets or carriers thoroughly immediately following a tube or well plate breakage.

1-28

GX-TB-004EE

Page 39

SX4750A Swinging-Bucket Rotor

Care and Maintenance

Aerosolve Cannisters

WARNING

When working with potentially hazardous materials, open cannisters in an appropriate hood or biological safety cabinet in case of tube breakage.

If a glass tube breaks in an Aerosolve cannister tube rack, discard and replace the O-ring and rubber tube cushion in the base of the rack. Imbedded glass particles that remain in the bucket or adapters can break tubes during subsequent runs.

SST or Corvac Tubes

If an SST or Corvac tube breaks, the tube’s silicone gel barrier material becomes laced with glass fragments and the tube contents contaminate the entire tube adapter and bucket (or tube rack and cannister).

If one of these tubes breaks in an adapter in a bucket, use the following procedure.

Fill the bucket with water.

Autoclave the entire bucket and contents at 121°C for 20 minutes.

Decant any remaining water and dislodge the tube adapter (do not remove it) over a waste container.

Working under a hood and using plastic gloves, immerse the bucket and tube adapter (intact) in a beaker or similar vessel containing ethyl acetate.

Scrub the adapter with a brush and decant the ethyl acetate mixture into a waste bottle for proper disposal.

Rinse with water and air-dry the bucket and tube adapter.

If one of these tubes breaks in an Aerosolve cannister, use the following procedure.

Working in a biologic safety cabinet and wearing plastic gloves, open the cannister and immerse the cannister, lid, and contents (intact) in a beaker or similar vessel containing 6%

sodium hypochlorite (undiluted bleach).

* Flammability hazard. Do not use in or near operating centrifuges.

GX-TB-004EE

1-29

Page 40

SX4750A Swinging-Bucket Rotor

Returning a Rotor

Leave the cannister to soak overnight.

Carefully dislodge the tube rack and scrub it with a brush.

Decant the bleach into a waste bottle for proper disposal.

Rinse with water and air-dry the cannister and tube rack.

Storage

When it is not in use, store the rotor in a dry environment (not in the centrifuge).

Returning a Rotor

Before returning a rotor or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. This form may be obtained from your local Beckman Coulter sales office. The form, entitled Returned Material Authorization (RMA) for United States returns or Returned Goods Authorization (RGA) for international returns, should contain the following information:

• rotor type and serial number,

• history of use (approximate frequency of use),

• reason for the return,

• original purchase order number, billing number, and shipping number, if possible,

• name and email address of the person to be notified upon receipt of the rotor or accessory at

the factory,

• name and email address of the person to be notified about repair costs, etc.

To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity. Sterilization and decontamination must be done before returning the parts. Smaller items (such as tubes, bottles, etc.) should be enclosed in a sealed plastic bag.

All parts must be accompanied by a note, plainly visible on the outside of the box or bag, stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach

this notification will result in return or disposal of the items without review of the reported problem.

Use the address label printed on the RGA/RMA form when mailing the rotor and/or accessories.

Customers located outside the United States should contact their local Beckman Coulter office.

1-30

GX-TB-004EE

Page 41

Supply List

SX4750A Swinging-Bucket Rotor

Supply List

NOTE

Publications referenced in this manual can be obtained at www.beckmancoulter.com or by calling Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local Beckman Coulter office.

Call Beckman Coulter Sales (1-800-742-2345 in the United States) for detailed information on ordering parts and supplies. For your convenience, a partial list is given below.

Replacement Rotor Parts

SX4750A rotor assembly (tube-and-bottle buckets) 369704

Tube-and-bottle bucket (set of 2) 392804

Tube-and-bottle bucket cover (set of 2) 392805

Tube-and-bottle bucket O-ring (pkg/8) 961648

Tube-and-bottle bucket cover latch assembly 360587

Multiwell-plate carrier (set of 2) 392806

Multiwell-plate carrier cover (set of 2) 393071

Multiwell-plate carriage (set of 2) 392873

Multiwell-plate support pad (set of 4) 392872

Bucket/carrier cover air-vent filter (set of 60) 368148

Tie-down T-bar 369718

Other

NOTE

For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com

Adapters see Table 1

Tubes and bottles see Table 2

Multiwell-plate labware see Table 3

Soft rubber roller (for aluminum foil lids) 538618

Tube decanter (for use with blue adapter)

includes:

gasket for 10-mm dia tubes (silicone) gasket for 12-mm dia tubes (silicone)

Adapter plate,1.5-mL (for use with blue adapter) 354511

Aerosolve cannister kit (set of 4)

(each kit includes 1 cannister, 1 lid, 1 O-ring, silicone

vacuum grease, and 1 pad) 359232

Aerosolve cannister O-ring (ethylene propylene) 345366

Aerosolve pad (placed beneath cannister in bucket)

(polyethylene) 361269

Tube racks for Aerosolve cannisters See Table 1

343108

343106 343107

GX-TB-004EE

1-31

Page 42

SX4750A Swinging-Bucket Rotor

Supply List

Neoprene tube cushions

for blue rack (pkg of 24) for orange rack (pkg of 18) for purple rack (pkg of 18; 12 large, 6 small) for dark green rack (pkg of 18; 12 large, 6 small) for yellow rack (pkg of 8; 4 large, 4 small)

Blood bag cup, 90-mm, yellow (polypropylene) 356856

Blood bag cup, 97-mm, orange (polypropylene) 356857

Balancing pads (six pads, 3 grams each, red) 358365

Cell culture flask adapter, 75-cm

Cell culture flask adapter, 25-cm

Rotor Cleaning Kit 339558

Solution 555 (1 qt) 339555

Silicone vacuum grease (1 oz) 335148

Spinkote lubricant (2 oz) 306812

Paint On Graphite Lubricant 977212

(pkg of 2)

344117 344118 344119 344120 344121

369292

369295

1-32

GX-TB-004EE

Page 43

Beckman Coulter, Inc.

SX4750A Warranty

Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election, by replacement, any defects of material or workmanship which develop within seven (7) years after delivery of a benchtop centrifuge rotor to the original buyer by Beckman Coulter or by an authorized representative, provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use. Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this warranty, Beckman Coulter will supply free of charge all centrifuge parts required for repair.

Conditions

Except as otherwise specifically provided herein, this warranty covers the rotor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to (i) tubes, (ii) tube caps, (iii) tube adapters, or (iv) tube contents.

This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual.

This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor characteristics or operated in a Beckman Coulter centrifuge that has been improperly disassembled, repaired, or modified.

Thermoplastic rotors or components used in some benchtop centrifuges are warranted for one (1) year from date of purchase.

Disclaimer

IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER, INC. SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.

GX-TB-004EE

Warranty-1

Page 44

Beckman Coulter, Inc. SX4750A Warranty

Warranty-2

GX-TB-004EE

Page 45

Page 46

www.beckmancoulter.com

Beckman Coulter SX4750A Instruction For Use, Installation And Assembly

Specifications and Main Features

Frequently Asked Questions

User Manual