Beckman Coulter SW 32 Ti, SW 32.1 Ti Instructions For Use Manual

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Page 1

Instructions For Use

Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

For Use in Beckman Coulter Class H, R, and S Preparative Ultracentrifuges

LXL-TB-015BB November 2011

Page 2

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Beckman Coulter Ireland, Inc. Mervue Business Park, Mervue Galway, Ireland 353 91 774068

製造販売元 : ベックマン･コールター株式会社東京都江東区有明三丁目5番7号 TOC 有明ウエストタワー

贝克曼库尔特有限公司，美国加利福尼亚州，Brea 市，S. Kraemer 大街 250 号，邮编：92821 电话：(001) 714-993-5321

LXL-TB-015AE (November 2011)

All rights reserved. No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without prior written permission from Beckman Coulter, Inc.

Find us on the World Wide Web at:

www.beckmancoulter.com

Page 3

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting

to use this equipment. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.

This safety notice summarizes information basic to the safe use of the rotors described in this manual. The international symbol displayed to the left is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equipment is attempted. When you see the symbol on other pages of this publication, pay special attention to the safety information presented. Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor. These rotors were developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter ultracentrifuge/ rotor system. Their safety or reliability cannot be assured if used in an ultracentrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter ultracentrifuge that has been modified without Beckman Coulter’s approval.

Alerts for Danger, Warning, Caution, and Note

Safety Notice

DANGER

DANGER indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

NOTE

NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

Safety Information for the SW 32 Ti and SW 32.1 Ti Rotors

Handle body fluids with care because they can transmit disease. No known test offers complete assurance that such fluids are free of micro-organisms. Some of the most virulent —Hepatitis (B and C) viruses, HIV (I–V), atypical mycobacteria, and certain systemic fungi—further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory

LXL-TB-015BB

iii

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Safety Notice

Safety Information for the SW 32 Ti and SW 32.1 Ti Rotors

procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in these rotors without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

The rotors and accessories are not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials in nor handle or store them near the centrifuge

Although rotor components and accessories made by other manufacturers may fit in the SW 32 Ti and SW 32.1 Ti rotors, their safety in these rotors cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in these rotors may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the components and accessories listed in this publication should be used in this rotor.

Attach all six buckets, loaded or empty, to the rotor for every run. Make sure that filled containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same level with liquid of the same density. Make sure that buckets containing Quick-Seal tubes have the proper floating spacers inserted (if applicable) before installing the bucket cap.

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section on Run

Speeds, and derate the run speed as appropriate.

Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins in scratches and may open fissures in the rotor with continued use.

LXL-TB-015BB

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Contents

Safety Notice,iii

Alerts for Danger, Warning, Caution, and Note, iii

Safety Information for the SW 32 Ti and SW 32.1 Ti Rotors,iii

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors,1

Specifications for the SW 32 Ti Rotor,1

Specifications for the SW 32.1 Ti Rotor,2

Description,3

Preparation and Use,4

Prerun Safety Checks,4 Rotor Preparation,4 Operation,6 Removal and Sample Recovery,7 Tubes and Accessories,7 Run Times,12 Run Speeds,13 Selecting CsCl Gradients,19 Adjusting Fill Volumes,19 Typical Examples for Determining CsCl Run Parameters,20

Care and Maintenance,21

Maintenance,21 Cleaning,22 Decontamination,23 Sterilization and Disinfection,23 Storage,23

Returning a Rotor,23

Supply List,24

Replacement Rotor Parts,24 Other,25

Warranty

Page 6

Page 7

Illustrations

1 Arranging Tubes Symmetrically in the Rotor Buckets,6 2 Precipitation Curves for the SW 32 Ti Rotor.r,15 3 CsCl Gradients at Equilibrium for the SW 32 Ti Rotor,16 4 Precipitation Curves for the SW 32.1 Ti Rotor,17 5 CsCl Gradients at Equilibrium for the SW 32.1 Ti ,18

Illustrations

vii

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Tables

1 Beckman Coulter Tubes and Accessories for the SW 32 Ti

Rotor,8

2 Beckman Coulter Tubes and Accessories for the SW 32.1 Ti

Rotor,9

3 Relative Centrifugal Fields for the SW 32 Ti and SW 32.1 Ti

Rotors,14

viii

Page 9

Specifications for the SW 32 Ti Rotor

min

max

Maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32,000 RPM

Density rating at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL

Relative Centrifugal Field

At r

k factor at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 204

k factor at maximum speed (5 to 20% sucrose gradient; 5°C)

(152.5 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175,000  g

max

(109.7 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126,000  g

(66.8 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76,600  g

min

SW 32 Ti and SW 32.1 Ti

Swinging-Bucket Rotors

at maximum speed

When particle density = 1.3 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 468

When particle density = 1.5 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 428

When particle density = 1.7 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 412

Conditions requiring speed reductions . . . . . . . . . . . . . . . . . . . . see Run Speeds

Number of buckets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Available tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 1

Nominal tube dimensions (largest tube) . . . . . . . . . . . . . . . . . . . . . 25  89 mm

1. Axis of Rotation

a. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rw2) to the standard acceleration of

gravity (g) according to the following formula: RCF = r/g — where r is the radius in millimeters,  is the angular velocity in radians per

second (2  RPM /60), and g is the standard acceleration of gravity (9807 mm/s

Nominal tube capacity (largest tube) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38.5 mL

Nominal rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 231 mL

Approximate acceleration time to maximum speed

(rotor fully loaded). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Approximate deceleration time from maximum speed

(rotor fully loaded . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Weight of fully loaded rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.5 kg (18.7 lb)

Rotor and bucket material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . titanium

). After substitution: RCF = 1.12r (RPM/1000)

/4 min

/2min

LXL-TB-015BB

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SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

min

max

Specifications for the SW 32.1 Ti Rotor

Maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32,000 RPM

Density rating at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL

Relative Centrifugal Field

At r

k factor at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 229

k factor at maximum speed (5 to 20% sucrose gradient; 5°C)

When particle density = 1.3 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 613

When particle density = 1.5 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 560

When particle density = 1.7 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 536

(162.8 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 187,000  g

max

(113.6 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130,000  g

(64.4 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73,900  g

min

at maximum speed

Conditions requiring speed reductions . . . . . . . . . . . . . . . . . . . . see Run Speeds

Number of buckets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

1. Axis of Rotation

Available tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 2

Nominal tube dimensions (largest tube) . . . . . . . . . . . . . . . . . . . . 16  102 mm

Nominal tube capacity (largest tube) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 mL

Nominal rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102 mL

Approximate acceleration time to maximum speed

(rotor fully loaded). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

/2 min

Approximate deceleration time from maximum speed

(rotor fully loaded . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

/4 min

Weight of fully loaded rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.3 kg (18.3 lb)

Rotor and bucket material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . titanium

a. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rw2) to the standard acceleration of

gravity (g) according to the following formula: RCF = r/g — where r is the radius in millimeters,  is the angular velocity in radians per

second (2  RPM /60), and g is the standard acceleration of gravity (9807 mm/s

). After substitution: RCF = 1.12r (RPM/1000)

LXL-TB-015BB

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Description

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Description

1. Black Cap

2. O-ring (812715)

3. Bucket (SW 32 Ti)

4. Rotor

5. Overspeed Disk (335456)

These Beckman Coulter rotors have been manufactured in an ISO 9001 or 13485 facility for use with the specified Beckman Coulter ultracentrifuges.

The SW 32 Ti and SW 32.1 Ti are swinging bucket rotors designed to centrifuge up to six tubes each. Used in Beckman Coulter class H, R, and S preparative ultracentrifuges, these rotors develop centrifugal forces for the separation of subcellular particles and viruses in density gradients. The rotors have a common rotor body with buckets that can be used interchangeably (see

Rotor

Preparation). Bucket and rotor body positions are numbered for operator convenience.

The rotor body and buckets are made of titanium, finished with polyurethane paint. Bucket caps are made of aluminum, anodized for corrosion resistance. Each bucket and cap assembly sets into grooves in the rotor body. O-rings, made of Buna N rubber, between each bucket and bucket cap maintain atmospheric pressure inside the buckets during centrifugation.

For overspeed protection, a Beckman Coulter ultracentrifuge equipped with a photoelectric detector will monitor the overspeed disk on the rotor bottom and shut down the run if a speed exceeding the maximum allowable run speed is detected.

See the Warranty at the back of this manual for warranty information.

LXL-TB-015BB

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SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

32,000-RPM

56-Sector

(335456)

Preparation and Use

Specific information about the SW 32 Ti and SW 32.1 Ti rotors is given here. Information common to these and other rotors is contained in Rotors and Tubes for Preparative Ultracentrifuges (publication LR-IM), which should be used together with this manual for complete rotor and accessory operation. Publication LR-IM is included in the literature package with this rotor manual.

NOTE

Prerun Safety Checks

Read the Safety Notice section at the front of this manual before using the rotor.

Make sure that the rotor, buckets, and caps are clean and show no signs of corrosion or cracking.

Make sure that the rotor is equipped with the correct overspeed disk.

a. If the disk is missing or damaged, replace it according to the instructions in Rotors

and Tubes

Verify that the tubes and bottles being used are listed in Table 1 or Table 2.

Check the chemical compatibilities of all materials used.

• Refer to Chemical Resistances (publication IN-175), included in the Rotors and Tubes CD.

Rotor Preparation

For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration.

NOTE

Place the rotor on the rotor stand (332400) when it is not in the centrifuge. Take care to protect the overspeed disk from damage when handling the rotor.

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SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

Install the rotor body on the drive hub.

a. Slowly turn the rotor to the right (clockwise) to make sure it is properly seated.

Load the filled containers into the buckets (see page 7 for tube and accessory information).

a. Complete loading by placing the correct floating spacers (if required) over the tubes.

Ensure that bucket gaskets are lightly but evenly coated with silicone vacuum grease.

a. Do not run a bucket without an O-ring, as the bucket will leak.

1. Ta b

2. Black Cap

3. O-ring (812715)

4. SW 32 Ti Bucket

5. Ta b

6. Red Cap

7. O-ring (812715)

8. SW 32.1 Ti Bucket

CAUTION

Use only black caps (369643) on black SW 32 Ti buckets and red caps (369645) on red SW 32.1 Ti buckets.

Use a lint-free cotton swab to apply Spinkote™ lubricant (396812) to cap grooves in the bucket tops.

a. Match bucket caps with numbered buckets. b. Align the pins on each side of the cap with the guide slots in the bucket. c. Twist the cap clockwise until it stops (one-quarter turn).

1. Cap Pins

2. Ta b

3. Bucket Top View

4. Guide Slots

5. This side toward outside of rotor

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SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

Grasp the bucket cap tabs and lower the buckets into the openings on the rotor body with the rounded sides toward the outside of the rotor.

Operation

NOTE

Six bucket assemblies must be installed, whether loaded or empty. If fewer than six tubes are being run, they must be arranged symmetrically in the rotor (see Figure 1). Opposing tubes must be filled to the same level with liquid of the same density.

Figure 1 Arranging Tubes Symmetrically in the Rotor Buckets

NOTE Two, three, four, or six tubes can be centrifuged per run if they are arranged in the rotor as shown. All

bucket assemblies must be attached to the rotor, whether loaded or empty.

A bucket will not seat in the rotor if it is inserted in the rotor with the rounded side toward the rotor

center. The bucket will fall through, spilling or disturbing the tube contents.

For low-temperature runs, precool the rotor in the centrifuge or in a refrigerator before use—especially before short runs—to ensure that the rotor reaches the set temperature. A suggested precooling cycle is a minimum of 30 minutes at 2000 RPM at the required temperature.

Refer to the centrifuge instruction manual for additional operating instructions.

For additional operating information, see the following:

• Run Times, page 12, for using k factors to adjust run durations.

• Run Speeds, page 13, for information about speed limitations.

• Selecting CsCl Gradients, page 19, for methods to avoid CsCl precipitation during

centrifugation.

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Page 15

Removal and Sample Recovery

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

Grasp the bucket cap tabs and lift up to remove the buckets.

• (Buckets may be removed without removing the rotor body from the ultracentrifuge.)

Remove the bucket caps by twisting counterclockwise and lifting up.

a. Use a tube removal tool (361668) or hemostat to remove the spacers and tubes.

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

NOTE

To remove the rotor from the centrifuge, lift the rotor straight up and off the drive hub.

a. Set the rotor on the rotor stand.

If the conical-shaped adapters that support konical tubes are difficult to remove after

centrifugation, an extractor tool (354468) is available to facilitate removal.

Tubes and Accessories

The SW 32 Ti rotor uses tubes and accessories listed in Table 1; the SW 32.1 Ti rotor uses tubes and accessories listed in Table 2. Be sure to use only those items listed, and to observe the maximum speed limits shown. Refer to Appendix A in Rotors and Tubes for information on the chemical resistances of tube and accessory materials.

1. Extractor Tool (354468)

While pressing the rubber tip against the adapter wall, pull the tube and adapter up and out of the cavity.

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SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

Table 1 Beckman Coulter Tubes and Accessories for the SW 32 Ti Rotor

Tube Required Accessory

Dimensions/

Nominal Volume/

25  89 mm

38.5 mL

25  89 mm

38.5 mL

25  89 mm

31 mL

Description

Ultra Clear open-top

polyallomer open-top

thickwall polyallomer

Part

Number

344058

(pkg/50)

326823

(pkg/50)

355642

(pkg/25)

Description

none — 32,000 RPM

open-top

25  89 mm

31 mL

thickwall polycarbonate

355631

(pkg/25)

none — 32,000 RPM

open-top

Max Speed/

Part

Number

RCF/

k factor

175,000  g

204

175,000  g

204

175,000  g

198

175,000  g

202

25  83 mm

33.5 mL

25  89 mm

31.5 mL

25  83 mm

28 mL

25  76 mm

25.5 mL

25  64 mm

27 mL

25  77 mm

32.4 mL

25  38 mm

15 mL

polyallmer Quick-Seal, bell-top

konical polyallomer open-top

konical polyallomer Quick-Seal,bell-top

konical polyallomer open-top

polyallomer Quick-Seal, bell-top

OptiSeal bell-top

polyallomer Quick-Seal, bell-top

344623

(pkg/50)

358126

(pkg/50)

358651

(pkg/50)

358125

(pkg/50)

343665

(pkg/50)

361625

(pkg/50)

343664

(pkg/50)

Noryl spacer

floating

355536 32,000 RPM

175,000  g

192

Hytrel

adapter

358156

(pkg/6)

32,000 RPM

175,000  g

199

adapter 358156 32,000 RPM

175,000  g

Noryl floating spacer 355536

Hytrel adapter 358156

(pkg/6)

198

32,000 RPM

175,000  g

156

Noryl floating spacer 355536 32,000 RPM

175,000  g

146

Ultem spacer

392833 32,000 RPM

175,000  g

155

Noryl floating spacer 355536 32,000 RPM

175,000  g

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Page 17

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

Table 1 Beckman Coulter Tubes and Accessories for the SW 32 Ti Rotor

Tube Required Accessory

Dimensions/

Nominal

Volume/

25  38 mm

8.4 mL

Description

konical polyallomer Quick-Seal, bell-top

Part

Number

358652

(pkg/50)

Description

Hytrel adapter 358156

Noryl floating spacer 355536

25  76 mm

22.5 mL

konical polyallomer Quick-Seal, bell-top

358654

(pkg/50)

Hytrel adapter 358156 32,000 RPM

Noryl floating spacer 355536

a. Use only the items listed here..

b. Noryl and Ultem are registered trademarks of GE Plastics.

c. Hytrel is a registered trademark of E.I. Du Pont de Nemours and Company.

d. Includes disposable plastic plugs.

Table 2 Beckman Coulter Tubes and Accessories for the SW 32.1 Ti Rotor

(Continued)

Number

Part

(pkg/6)

Max Speed/

RCF/

k factor

32,000 RPM 175,000  g

175,000  g

155

Dimensions/

Nominal

Volume/

16  102 mm

14.5 mL

16  102 mm

17 mL

16  102 mm

12.5 mL

16  96 mm

16.5 mL

16  93 mm

13.5 mL

Tube Required Accessory

Part

Description

polyallomer Quick-Seal,

Number

356291

(pkg/50)

Description

Noryl floating spacer 355579 32,000 RPM

bell-top

Ultra-Clear, open-top

konical polyallomer Quick-Seal bell-top

344061

(pkg/50)

358653

(pkg/50)

none — 32,000 RPM

adapter 358155 32,000 RPM

Noryl floating spacer 355579

polyallomer, open-top

konical polyallomer open-top

337986

(pkg/50)

358123

(pkg/50)

none — 32,000 RPM

Hytrel adapter 358155 32,000 RPM

Part

Number

Max Speed/

RCF/

k factor

187,000  g

199

187,000  g

228

187,000  g

205

187,000  g

228

187,000  g

225

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Page 18

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

4°C

Preparation and Use

Table 2 Beckman Coulter Tubes and Accessories for the SW 32.1 Ti Rotora(Continued)

Tube Required Accessory

Dimensions/

Nominal Volume/

16  67 mm

10.5 mL

16  57 mm

8 mL

16  44 mm

6.5 mL

16  32 mm

4.5 mL

a. Use only the items listed here..

Description

polyallomer Quick-Seal, bell-top

Part

Number

344622

(pkg/50)

344621

(pkg/50)

345830

(pkg/50)

356562

(pkg/50)

Part

Description

Noryl floating spacer 355579 32,000 RPM

Number

Max Speed/

RCF/

k factor

187,000  g

133

187,000  g

102

187,000  g

Temperature Limits

• Plastic tubes have been centrifuge tested for use at temperatures between 4 and 25°C. For

centrifugation at other temperatures, pretest tubes under anticipated run conditions.

• If plastic containers are frozen before use, make sure that they are thawed to at least 4°C prior to centrifugation.

OptiSeal Tubes

OptiSeal™ tubes come with plastic plugs and can be quickly and easily prepared for use. With the tube spacer in place, the g force during centrifugation ensures a tight, reliable seal that protects your samples.

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Page 19

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

Place the tubes in the rack and fill each tube to the base of the stem, leaving no fluid in the stem.

• Overfilling the tube can cause spillage when the plug is inserted or can compromise seal integrity.

• However, too much air can cause excessive tube deformation, disrupting gradients and sample bands.

1. Spacer

2. Plug

3. Stem

4. Meniscus

5. Tu b e

6. Base of Stem

Refer to Using OptiSeal™ Tubes (publication IN-189), included in each box of tubes, for detailed information on the use and care of OptiSeal tubes.

Quick Seal Tubes

Quick-Seal® tubes must be sealed prior to centrifugation. These tubes are heat sealed and do not need caps; however, spacers are required on top of the tubes when they are loaded into the rotor buckets.

Fill Quick-Seal tubes leaving a small bubble of air at the base of the neck.

a. Do not leave a large air space—too much air can cause excessive tube deformation.

Some of the tubes listed in Table 1 and Table 2 are part of the g-Max™ system, which uses a combination of small bell-top Quick-Seal tubes and floating spacers (also called g-Max spacers).

• This means that you can run the shorter tubes listed in the Tables in the SW 32 Ti and SW 32.1 T rotors without reduction in g force.

• For detailed information on the g-Max system see publication DS-709.

1. g-Max Spacer

2. Bell-top Tube

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Page 20

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

maxrmin

ln



-----------------------------------

3600

------------

=

2.533 10

r

max

r

min

ln

RPM

--------------------------------------------- ---------------------------=

Preparation and Use

Refer to Rotors and Tubes for detailed information on the use and care of Quick-Seal tubes.

• Quick-Seal tubes are disposable and should be discarded after a single use.

konical™ Tubes

Polyallomer konical tubes, used to optimize pelleting separations, have a conical tip that concentrates the pellet in the narrow end of the tube. The narrow bottom also reduces the tube’s nominal volume and minimizes gradient material requirement. The konical tubes come in both open-top and Quick-Seal tube designs. Conical cavity adapters hold the tubes in the rotor buckets

1. Adapters

Run Times

Polyallomer and Ultra-Clear Open-Top Tubes

Polyallomer and Ultra-Clear® open-top tubes should be filled as full as possible (2 or 3 mm from the tube top) for tube support. If necessary, float mineral oil (or some other low-density, immiscible liquid) on top of the tube contents to fill the tube to its maximum volume. (Do not use an oil overlay in Ultra-Clear tubes.) All opposing tubes for a run must be filled to the same level with liquid of the same density.

The k factor of the rotor is a measure of the rotor’s pelleting efficiency. (Beckman Coulter has calculated the k factors for all of its preparative rotors at maximum rated speed and using full tubes.) The k factor is calculated from the formula

EQ 1

where  is the angular velocity of the rotor in radians per second ( = 0.105  RPM), r maximum radius, and r

is the minimum radius.

min

After substitution:

max

is the

EQ 2

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Page 21

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

k s

---=

adj

32 000

actual run speed

----------------------------------------





-----

------=

reduced maximum speed = 32,000 RPM)

1.2 g/mL



----------------------

Preparation and Use

Use the k factor in the following equation to estimate the run time t (in hours) required to pellet particles of known sedimentation coefficient s (in Svedberg units, S).

EQ 3

Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows:

EQ 4

Run times can also be estimated from data established in prior experiments if the k factor of the previous rotor is known. For any two rotors, a and b:

EQ 5

where the k factors have been adjusted for the actual run speed used.

Run Speeds

The centrifugal force at a given radius in a rotor is a function of speed. Comparisons of forces between different rotors are made by comparing the rotors’ relative centrifugal fields (RCF). When rotational speed is selected so that identical samples are subjected to the same RCF in two different rotors, the samples are subjected to the same force. The RCF at a number of rotor speeds is provided in Table 3.

Do not select rotational speeds in excess of 32,000 RPM. In addition, speeds must be reduced under the following circumstances:

1. If nonprecipitating solutions more dense than 1.2 g/mL are centrifuged, reduce the maximum

2. Further speed limits must be imposed when CsCl or other self-forming-gradient salts are

allowable run speed according to the following equation:

EQ 6

where  is he density of the tube contents. This speed reduction will protect the rotor from excessive stresses due to the added tube load.

centrifuged, as equation (6) does not predict concentration limits/speeds that are required to prevent precipitation of salt crystals. Solid CsCl has a density of 4 g/mL, and if precipitated during centrifugation may cause rotor failure. Figure 2 through Figure 5, together with the description and examples below, show how to reduce run speeds when using CsCl gradients.

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Page 22

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

Rotor Speed (RPM)

5,000

500

(162.8 mm)

Relative Centrifugal Field (g)

At r

max

(113.6 mm)

187,000

164,000

114,000

73,000

41,000

18,200

4,560

Table 3 Relative Centrifugal Fields for the SW 32 Ti and SW 32.1 Ti Rotors

Relative Centrifugal Field (g)

Rotor Speed (RPM)

32,000

30,000

25,000

20,000

15,000

10,000

5,000

500

a. Entries in this table are calculated from the formula RCF = 1.12r (RPM/1000)2 and then rounded to three significant digits.

At r

max

(152.5 mm)

175,000

154,000

107,000

68,300

38,400

17,100

4,270

At r

(109.7 mm)

126,000

111,000

76,800

49,200

27,600

12,300

3,070

At r

min

(66.8 mm)

76,600

67,300

46,800

29,900

16,800

7,480

1,870

32,000

30,000

25,000

20,000

15,000

10,000

Relative Centrifugal Fields, SW 32 Ti Rotor

200,000

180,000

160,000

140,000

120,000

100,000

80,000

RCF (x g)

60,000

40,000

20,000

0 5000 10,000 15,000 20,000 25,000 30,000

Speed (RPM)

32,000

At r

130,000

115,000

80,000

50,900

28,600

12,700

3,180

At r

(64.4 mm)

73,900

65,000

45,100

28,900

16,200

max

min

7,210

1,800

200,000

180,000

160,000

140,000

120,000

100,000

80,000

RCF (x g)

60,000

40,000

20,000

0 5000 10,000 15,000 20,000 25,000 30,000

Relative Centrifugal Fields, SW 32.1 Ti Rotor

Speed (RPM)

32,000

max

min

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Page 23

Figure 2 Precipitation Curves for the SW 32 Ti Rotor.r*

1.90

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

1.80

1.70

1.60

1.50

Homogeneous CsCl Solution (g/mL)

1.40

1/4

1/2

3/4

full

1/4

1/2

3/4

full

1.30

1.20

1.10 0

* Using speed and density combinations that intersect on or below the solid curves ensures that CsCl will not precipitate

during centrifugation. Tube fill volumes are indicated on the curves.

LXL-TB-015BB

SW 32 Ti ROTOR

= 20°C

= 4°C

5000 10,000 15,000 20,000 25,000 30,000

Rotor Speed (RPM)

Page 24

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

1.20

1.10

1.00

1.30

1.40

1.50

1.60

1.70

1.80

1.90

Density (g/mL)

max

70.0 80.0 90.0 100.0 110.0 120.0 130.0 140.0 152.5

Distance from Axis of Rotation (mm)

min 3/4-filled 1/2-filled 1/4-filled

66.8

SW 32 Ti ROTOR

= 20°C

= 4°C

20,000 RPM

25,000 RPM

30,000 RPM

32,000 RPM

Preparation and Use

Figure 3 CsCl Gradients at Equilibrium for the SW 32 Ti Rotor

* Centrifugation of homogeneous CsCl solutions at maximum allowable speeds (from Figure 2) results in gradients

presented here.

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Page 25

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

Figure 4 Precipitation Curves for the SW 32.1 Ti Rotor

1.90

1.80

1.70

1.60

1.50

1/4

1/2

3/4

full

1/4

1/2

3/4

Homogeneous CsCl Solution (g/mL)

1.40

1.30

1.20

1.10 0

5000 10,000 15,000 20,000 25,000 30,000

full

SW 32.1 Ti ROTOR

= 20°C

= 4°C

Rotor Speed (RPM)

* Using speed and density combinations that intersect on or below the solid curves ensures that CsCl will not precipitate

during centrifugation. Tube fill volumes are indicated on the curves.

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Page 26

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

1.20

1.10

1.00

1.30

1.40

1.50

1.60

1.70

1.80

1.90

Density (g/mL)

max

70.0 80.0 90.0 100.0 110.0 120.0 130.0 140.0 150.0

Distance from Axis of Rotation (mm)

min

64.4 162.8

SW 32.1 Ti ROTOR

= 20°C

= 4°C

20,000 RPM

25,000 RPM

30,000 RPM

32,000 RPM

25,000 RPM

3/4-filled 1/2-filled 1/4-filled

Preparation and Use

Figure 5 CsCl Gradients at Equilibrium for the SW 32.1 Ti

* Centrifugation of homogeneous CsCl solutions at maximum allowable speeds (from Figure 4) results in gradients

presented here.

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Page 27

Selecting CsCl Gradients

Rotor speed is used to control the slope of a CsCl density gradient, and must be limited to prevent CsCl precipitation during centrifugation. Speed and density combinations that intersect on or below the curves in Figure 2 (for the SW 32 T i rotor) a nd in Figure 4 (for the SW 32.1 Ti rotor) ensure that CsCl will not precipitate during centrifugation in these rotors. Curves are provided at two temperatures: 20°C (black curves) and 4°C (gray curves). Curves in Figure 2 through Figure 5 are provided up to the maximum speed of the rotor.

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Preparation and Use

NOTE

The curves in Figure 2 through Figu re 5 are for solutions of CsCl salt dissolved in distilled water only. If other salts are present in significant concentrations, the overall CsCl concentration may need to be reduced.

The reference curves shown in Figure 3 and Figure 5 show gradient distribution at equilibrium. Each curve inFigure 3 is within the density limits allowed for the SW 32 Ti rotor; each curve in

Figure 5 is within the density limits allowed for the SW 32.1 Ti rotor. Each curve was generated for

a single run speed using the maximum allowable homogeneous CsCl densities (one for each fill level) that avoid precipitation at that speed. (The gradients in Figure 3 and Figure 5 can be generated from step or linear gradients, or from homogeneous solutions. But the total amount of CsC l i n solut io n must b e e quivale nt to a homo ge neous solution corresponding to the concentrations specified in Figure 3 and Figure 5.) Figure 3 and Figure 5 can also be used to approximate the banding positions of sample particles. Curves not shown may be interpolated.

Adjusting Fill Volumes

Figure 2 through Figure 5 show that several fill volumes are possible in a tube. If a thinwall tube is

partially filled with gradient solution, float mineral oil (or some other low-density, immiscible liquid) on top of the tube contents to fill the tube to its maximum volume. (Do not use an oil overlay in Ultra-Clear tubes.) Note that for a given CsCl density, as the fill level decreases the maximum allowable speed increases. Partial filling may be desirable when there is little sample or when you wish to shorten the run time

For example, in the SW 32 Ti rotor, a quarter-filled tube of 1.69-g/mL homogeneous CsCl solution at 4°C may be centrifuged at 25,000 RPM (see Figure 2). The segment of the 25,000 RPM curve (Figure 3) from the quarter-filled line to r

a half-filled tube may be centrifuged no faster than 17,000 RPM, and 15,000 RPM in a three-quarter- filled tube. A tube full of the 1.69-g/mL CsCl solution may be centrifuged no faster than 13,500 RPM. Curves not shown in the figures may be interpolated

LXL-TB-015BB

(the tube bottom) represents this gradient. The same solution in

max

Page 28

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

min

max

V =  r2h

Preparation and Use

Typical Examples for Determining CsCl Run Parameters

Example A:

Starting with a homogeneous CsCl solution density of 1.40 g/mL and approximate particle buoyant densities of 1.38 and 1.42 g/mL, at 20°C, where will particles band at equilibrium in the SW 32 Ti rotor?

1. At Speed

2. At Rest in Rotor

3. At Rest Outside Rotor

4. Floating Components

5. Bands

6. Pelleted Material

7. Pathlength

In Figure 2, find the curve that corresponds to the required run temperature (20°C) and fill volume (three-quarters full).

• The maximum allowable rotor speed is determined from the point where this curve intersects the homogeneous CsCl density (28,000 RPM).

In Figure 3, sketch a horizontal line corresponding to each particle’s buoyant density.

Mark the point in Figure 3 where each particle density intersects the curve corresponding to the selected run speed and temperature.

• Particles will band at these locations across the tube diameter at equilibrium during centrifugation.

In this example, particles will band about 120 and 125 mm from the axis of rotation, about 5 mm of centerband-to-centerband separation.

To determine interband volume in milliliters, use the following equation:

EQ 7

where r is the tube radius in centimeters and h is the interband separation in centimeters

Example B:

Knowing particle buoyant densities (for example, 1.55 and 1.50 g/mL), how do you achieve good separation in the SW 32.1 Ti rotor?

In Figure 5, sketch in a horizontal line corresponding to each particle’s buoyant density.

LXL-TB-015BB

Page 29

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Care and Maintenance

Select the curve at the desired temperature (20°C) and tube volume (full) that gives the best particle separation.

Note the run speed along the selected curve (20,000 RPM).

From Figure 2, select the maximum homogeneous CsCl density (in this case, 1.56 g/mL) that corresponds to the temperature and run speed established above.

• These parameters will provide the particle-banding pattern selected in Step 2.

In this example, particles will band about 119.5 and 129 mm from the axis of rotation (about

9.5 mm apart).

Care and Maintenance

Maintenance

NOTE Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins

in scratches and may open fissures in the rotor with continued use.

Frequently check the bucket O-rings for signs of wear.

a. Replace O-rings every 6 months, or whenever worn or damaged. b. Use the hooked removal tool (978354) to remove O-rings. c. Keep the O-rings lightly coated with silicone vacuum grease (335148).

Regularly inspect the rotor body, buckets, and caps for cracks, pitting, or heavy discoloration.

NOTE

Wear will become visible over time on the contact areas between buckets and rotor body. Such

wear will not affect rotor operation.

LXL-TB-015BB

1. Contact Area

Page 30

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Care and Maintenance

Frequently lubricate the bucket and cap mating surfaces with a thin, even coat of Spinkote lubricant (306812).

Refer to Appendix A in Rotors and Tubes for the chemical resistances of rotor and accessory materials.

• Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center.

Cleaning

Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred. Do not allow corrosive materials to dry on the rotor.

Under normal use, wash the rotor frequently (at least weekly) to prevent buildup of residues.

Wash the rotor buckets, O-rings, and caps in a mild detergent, such as Beckman Solution 555™, that won’t damage the rotor.

a. Dilute the detergent 10 to 1 with water.

• The Rotor Cleaning Kit contains two plastic-coated brushes and two quarts of Solution 555

(339555) for use with rotors and accessories.

Rinse the cleaned rotor and components with distilled water.

Air-dry the rotor and buckets upside down. Do not use acetone to dry the rotor.

Air-dry the rotor and lid upside down.

a. Do not use acetone to dry the rotor.

Clean metal threads frequently to prevent buildup of residues and ensure adequate closure.

a. Use a brush and concentrated Solution 555. b. Rinse and dry thoroughly, then lubricate lightly but evenly with Spinkote to coat all

threads.

LXL-TB-015BB

Page 31

Decontamination

If the rotor or other components are contaminated with toxic, radioactive, or pathogenic materials, follow appropriate decontamination procedures as outlined by your laboratory safety officer. Refer to Appendix A in Rotors and Tubes to select solutions that will not damage the rotor and accessory materials.

Sterilization and Disinfection

• The rotor and all rotor components, except those made of Noryl, can be autoclaved at 121°C for

up to an hour. Remove the plugs from the rotor and place the rotor, plugs, and spacers in the autoclave upside down.

• Ethanol (70%) those made of plastic. Bleach (sodium hypochlorite) may be used, but may cause discoloration of anodized surfaces. Use the minimum immersion time for each solution, per laboratory standards.

or hydrogen peroxide (6%) may be used on all rotor components, including

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Returning a Rotor

While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

Refer to publication IN-192 (included with each box of tubes) for tube sterilization and disinfection procedures. Quick-Seal, Ultra Clear, and thinwall open-top tubes are disposable and should be discarded

after a single use.

Storage

When it is not in use, store the rotor and buckets in a dry environment (not in the instrument). Remove the bucket caps to allow air circulation so that moisture will not collect in the buckets.

Returning a Rotor

Before returning a rotor or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. This form may be obtained from your local Beckman Coulter sales office. The form, entitled Returned Material Authorization (RMA) for United States returns or Returned Goods Authorization (RGA) for international returns, should contain the following information:

• rotor type and serial number,

• history of use (approximate frequency of use),

• reason for the return,

* Flammability hazard. Do not use in or near operating ultracentrifuges.

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Page 32

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Supply List

• original purchase order number, billing number, and shipping number, if possible,

• name and email address of the person to be notified upon receipt of the rotor or accessory at

the factory,

• name and email address of the person to be notified about repair costs, etc.

To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity. Sterilization and decontamination must be done before returning the parts. Smaller items (such as tubes, bottles, etc.) should be enclosed in a sealed plastic bag.

All parts must be accompanied by a note, plainly visible on the outside of the box or bag, stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach

this notification will result in return or disposal of the items without review of the reported problem.

Use the address label printed on the RMA/RGA form when mailing the rotor and/or accessories.

Customers located outside the United States should contact their local Beckman Coulter office.

Supply List

NOTE Publications referenced in this manual can be obtained at www.beckmancoulter.com, by

calling Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local Beckman Coulter office.

See the Beckman Coulter Ultracentrifuge Rotors, Tubes & Accessories catalog (BR-8101, available at www.beckmancoulter.com) or contact Beckman Coulter Sales (1-800-742-2345 in the United States) for detailed information on ordering parts and supplies. For your convenience, a partial list is given below.

Replacement Rotor Parts

Description Part Number

SW 32 Ti rotor assembly 369650

SW 32 Ti buckets (set of 6, black, with caps and O-rings) 369647

SW 32 Ti bucket O-ring 812715

SW 32 Ti cap (black) 369643

SW 32.1 Ti rotor assembly 369651

SW 32.1 Ti buckets (set of 6, red, with caps and O-rings) 369648

SW 32.1 Ti bucket O-ring 812715

SW 32.1 Ti cap (red) 369645

Rotor stand 332400

Overspeed disk (32,000 RPM) 335456

LXL-TB-015BB

Page 33

Other

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.

Description Part Number

Tubes and accessories see Ta b l e 1 and

Ta b l e 2

Bucket holder rack 331186

Quick-Seal Cordless Tube Topper kit, 60 Hz 358312

Quick-Seal Cordless Tube Topper kit, 50 Hz (Europe) 358313

Quick-Seal Cordless Tube Topper kit, 50 Hz (Great Britain) 358314

Quick-Seal Cordless Tube Topper kit, 50 Hz (Australia) 358315

Quick-Seal Cordless Tube Topper kit, 50 Hz (Canada) 367803

Tu b e r ac k s f or t h e Tub e To p pe r

for 16-mm diameter tubes for 38-mm diameter tubes

348123 348124

Supply List

OptiSeal tube rack 361646

Tube removal tool (Quick-Seal tubes) 361668

Extractor tool (konical tube adapters) 354468

Spinkote lubricant (2 oz) 306812

Silicone vacuum grease (1 oz) 335148

Rotor Cleaning Kit 339558

Beckman Solution 555 (1 qt) 339555

Rotor cleaning brush 339379

Hooked O-ring removal tool 978354

LXL-TB-015BB

Page 34

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors

Supply List

LXL-TB-015BB

Page 35

Beckman Coulter, Inc.

Ultracentrifuge Rotor Warranty

All Beckman Coulter ultracentrifuge Fixed Angle, Vertical Tube, Near Vertical Tube, Swinging Bucket, and Airfuge rotors are warranted against defects in materials or workmanship for the time periods indicated below, subject to the Warranty Conditions stated below.

Preparative Ultracentrifuge Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 years — No Proration

Analytical Ultracentrifuge Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 years — No Proration

ML and TL Series Ultracentrifuge Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 years — No Proration

Airfuge Ultracentrifuge Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 year — No Proration

For Zonal, Continuous Flow, Component Test, and Rock Core Ultracentrifuge Rotors, see separate warranty.

Warranty Conditions (as applicable)

1. This warranty is valid for the time periods indicated above from the date of shipment to the original Buyer by Beckman Coulter or an authorized Beckman Coulter representative.

2. This warranty extends only to the original Buyer and may not be assigned or extended to a third person without written consent of Beckman Coulter.

3. This warranty covers the Beckman Coulter Centrifuge Systems only (including but not limited to the centrifuge, rotor, and accessories) and Beckman Coulter shall not be liable for damage to or loss of the user’s sample, non-Beckman Coulter tubes, adapters, or other rotor contents.

4. This warranty is void if the Beckman Coulter Centrifuge System is determined by Beckman Coulter to have been operated or maintained in a manner contrary to the instructions in the operator’s manual(s) for the Beckman Coulter Centrifuge System components in use. This includes but is not limited to operator misuse, abuse, or negligence regarding indicated maintenance procedures, centrifuge and rotor classification requirements, proper speed reduction for the high density of certain fluids, tubes, and tube caps, speed reduction for precipitating gradient materials, and speed reduction for high-temperature operation.

5. Rotor bucket sets purchased concurrently with or subsequent to the purchase of a Swinging Bucket Rotor are warranted only for a term co-extensive with that of the rotor for which the bucket sets are purchased.

6. This warranty does not cover the failure of a Beckman Coulter rotor in a centrifuge not of Beckman Coulter manufacture, or if the rotor is used in a Beckman Coulter centrifuge that has been modified without the written permission of Beckman Coulter, or is used with carriers, buckets, belts, or other devices not of Beckman Coulter manufacture.

7. Rotor parts subject to wear, including but not limited to rotor O-rings, VTi, NVT™, TLV, MLN, and TLN rotor tube cavity plugs and gaskets, tubing, tools, optical overspeed disks, bearings, seals, and lubrication are excluded from this warranty and should be frequently inspected and replaced if they become worn or damaged.

8. Keeping a rotor log is not mandatory, but may be desirable for maintenance of good laboratory practices.

Repair and Replacement Policies

1. If a Beckman Coulter rotor is determined by Beckman Coulter to be defective, Beckman Coulter will

repair or replace it, subject to the Warranty Conditions. A replacement rotor will be warranted for the time remaining on the original rotor’s warranty.

2. If a Beckman Coulter centrifuge is damaged due to a failure of a rotor covered by this warranty, Beckman Coulter will supply free of charge (i) all centrifuge parts required for repair (except the drive unit, which will be replaced at the then current price less a credit determined by the total number of revolutions or

LXL-TB-015BB

Warranty-1

Page 36

years completed, provided that such a unit was manufactured or rebuilt by Beckman Coulter), and (ii) if the centrifuge is currently covered by a Beckman Coulter warranty or Full Service Agreement, all labor necessary for repair of the centrifuge.

3. If a Beckman Coulter rotor covered by this warranty is damaged due to a malfunction of a Beckman Coulter ultracentrifuge covered by an Ultracentrifuge System Service Agreement, Beckman Coulter will repair or replace the rotor free of charge.

4. If a Beckman Coulter rotor covered by this warranty is damaged due to a failure of a Beckman Coulter tube, bottle, tube cap, spacer, or adapter, covered under the Conditions of this Warranty, Beckman Coulter will repair or replace the rotor and repair the instrument as per the conditions in policy point (2) above, and the replacement policy.

5. Damage to a Beckman Coulter rotor or instrument due to the failure or malfunction of a non-Beckman Coulter tube, bottle, tube cap, spacer, or adapter is not covered under this warranty, although Beckman Coulter will assist in seeking compensation under the manufacturer’s warranty.

Disclaimer

IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND BECKMAN COULTER, INC. SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.

Factory Rotor Inspection Service

Beckman Coulter, Inc., will provide free mechanical and metallurgical inspection in Indianapolis, Indiana, USA, of any Beckman Coulter rotor at the request of the user. (Shipping charges to Beckman Coulter are the responsibility of the user.) Rotors will be inspected in the user’s laboratory if the centrifuge in which they are used is covered by an appropriate Beckman Coulter Service Agreement. Contact your local Beckman Coulter office for details of service coverage or cost.

Before shipping, contact the nearest Beckman Coulter Sales and Service office and request a Returned Goods Authorization (RGA) form and packaging instructions. Please include the complete rotor assembly, with buckets, lid, handle, tube cavity caps, etc. A SIGNED STATEMENT THAT THE ROTOR AND ACCESSORIES ARE NON-RADIOACTIVE, NON-PATHOGENIC, NON-TOXIC, AND OTHERWISE SAFE TO SHIP AND HANDLE IS REQUIRED.

Warranty-2

LXL-TB-015BB

Page 37

Page 38

www.beckmancoulter.com

Beckman Coulter SW 32 Ti, SW 32.1 Ti Instructions For Use Manual

Specifications and Main Features

Frequently Asked Questions

User Manual