Beckman Coulter Allegra 6, Allegra 6R, Spinchron R, Allegra 6KR Instruction Manual

Allegra® 6 Series
and Spinchron™ R Centrifuges

Instruction Manual

BE- IM- 9

Symbol
Symbol
Symbole
Símbolo

Simbolo

!

Title / Titel / Titre / Titulo / Titolo /

Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione

Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate

/

On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)

Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)

Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra

Earth (ground)
Erde
Terre
Tierra
Scarica a terra

!

This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions should be read and understood before installation, operation, mainte
nance, or repair of this centrifuge. When you see the symbol on other pages, pay special
attention to the safety information presented. Observance of safety precautions will also
help to avoid actions that could damage or adversely affect the performance of the
centrifuge.

Safety During Installation and/or Maintenance

This centrifuge weighs 51.7 kg/114.0 lb (nonrefrigerated benchtop), 75.8 kg/167.0 lb
(refrigerated benchtop), or 90.3 kg/199.0 lb (refrigerated kneewell). DO NOT attempt
to

lift or move it without assistance from another person.

Be sure to use the anchoring system to secure the centrifuge in place. The anchoring
system is designed to reduce the possibility of injury or damage that could result from
centrifuge movement in the event of a major rotor mishap.

Any servicing of this equipment that requires removal of any covers can expose parts
which involve the risk of electric shock or personal injury. Make sure that the power
switch is off and the centrifuge is disconnected from the main power source, and refer
such servicing to qualified personnel.

SAFETY NOTICE

-

Do not replace any centrifuge components with parts not specified for use on this
instrument.

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and
plug to connect the centrifuge to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth­grounded. Check that the line voltage agrees with the voltage listed on the name-rating
plate affixed to the centrifuge.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple­outlet receptacle strip.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid
may get into the centrifuge and damage electrical or mechanical components.

Safety Against Risk of Fire

Certain electrical circuits within this equipment are protected by fuses against overcurrent
conditions. For continued protection against the risk of fire, replace only with the same
type and rating specified.

This centrifuge is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol)
in this centrifuge nor handle or store them within the 30-cm (1-ft) area surrounding the
centrifuge.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the rotors and accessories designed for use in this centrifuge.

• Before starting the centrifuge, make sure that the rotor tie-down nut (or rotor lid knob)
is securely fastened.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop the rotor by hand.

• Do not lift or move the centrifuge while the rotor is spinning.

• NEVER attempt to override the door interlock system while the rotor is spinning.

• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running.
During operation you should come within the envelope only to adjust instrument
controls, if necessary. Never lean on the centrifuge or place items on the centrifuge
while it is operating.

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic,
toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless
all necessary safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to
their use.

• Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that they are free of micro-organisms. Some of the most virulent

—

Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic

— further emphasize the need for aerosol protection. Handle other infectious

fungi
samples according to good laboratory procedures and methods to prevent spread of
disease. Because spills may generate aerosols, observe proper safety precautions for
aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this
centrifuge without taking appropriate safety precautions. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.

It is your responsibility to decontaminate the centrifuge and accessories before
requesting service by Beckman Coulter Field Service.

Allegra® 6 Series
and Spinchron™ R Centrifuges

Instruction Manual

BE-IM-9

February 2008

© 2008 Beckman Coulter, Inc.

INTRODUCTION

Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

Scope of Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi

Notes, Cautions, and Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi

CFC-Free Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

Canadian Regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii

Recycling Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii

SECTION 1: DESCRIPTION

Centrifuge Function and Specifications . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Centrifuge Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Allegra 6 and Spinchron Models . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3

Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

Safety Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5

Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6

Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6

Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6

Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6

Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6

Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7

Refrigeration System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7

Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8

Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8

Contents

Page

SECTION 2: INSTALLATION REQUIREMENTS

SECTION 3: OPERATION

Space and Location Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

Test Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

Run Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

Preparation and Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

Starting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4

Stopping a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6

iii

Contents

Page

SECTION 4: TROUBLESHOOTING

Shutdown/No-Start Diagnostics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

Other Possible Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Emergency Access . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

SECTION 5: CARE AND MAINTENANCE

General Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2

Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3

Sterilization and Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3

Tube Breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3

Speed Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4

Speed Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4

Speed Knob Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4

Temperature Knob Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6

Storage and Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7

Returning a Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7

Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8

Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8

Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8

Warranty

Illustrations and Tables

Figure 1-1. Allegra 6 Centrifuges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2

Figure 1-2. Interior View of the Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7

Figure 1-3. The Control Panel (Nonrefrigerated Model) . . . . . . . . . . . . . . . . . . . . 1-8

Figure 1-4. The Control Panel (Refrigerated Models) . . . . . . . . . . . . . . . . . . . . . . 1-9

Figure 2-1. Centrifuge Dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

Figure 4-1. Emergency Access . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5

Table 4-1. Shutdown/No-Start Diagnostics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Table 4-2. Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

iv

CERTIFICATION

Introduction

To ensure full system quality, Beckman Coulter Allegra® 6 series and

Spinchron

13485 facility. They have been designed and tested to be compliant

(when used with Beckman Coulter rotors) with the laboratory equip

ment requirements of applicable regulatory agencies. Declarations

conformity and certificates of compliance are available at

of

www.beckmancoulter.com.

™

R centrifuges have been manufactured in an ISO 9001 or

-

SCOPE OF MANUAL

This manual is designed to familiarize you with the Allegra 6 series

and Spinchron R centrifuges, their functions, specifications, opera

tion, and routine operator care and maintenance. We recommend that

you read this entire manual, especially the

safety-related information, before operating the centrifuge or

all

performing instrument maintenance.

NOTE

➠

The descriptions and instructions are the same
for Allegra 6R and Spinchron R centrifuges.
References in this manual to an Allegra 6R
model also apply to the Spinchron R model.

• Section 1 contains system specifications and a brief physical and
functional description of the centrifuge, including the operating
controls and indicators.

• Section 2 provides instructions for installing and connecting the
centrifuge.

SAFETY NOTICE and

-

• Section 3 contains centrifuge operating procedures.

v

Introduction

• Section 4 lists possible malfunctions, together with probable
causes and suggested corrective actions.

• Section 5 contains procedures for routine operator care and main­tenance, as well as a brief list of supplies and replacement parts.

NOTE

➠

If the centrifuge is used in a manner other
than

specified in this manual, the safety and
performance of this equipment could be
impaired. Further, the use of any equipment
other than that recommended by Beckman
Coulter has not been evaluated for safety. Use of
any equipment not specifically recommended in
this manual and/or the appropriate rotor manual
is the sole responsibility of the user.

CONVENTIONS

Certain symbols are used in this manual to call out safety-related and
other important information. These international symbols may also be
displayed on the centrifuge and are reproduced and described below
and on the inside of the front cover.

NOTES, CAUTIONS, AND WARNINGS

➠

!

NOTE

Used to call attention to important information
that should be followed during installation, use,
or servicing of this equipment.

CAUTION

Used to indicate a potentially hazardous situa­tion which, if not avoided, may result in minor or
moderate injury and/or mechanical damage. It
is also used to alert against unsafe practices.

vi

!

WARNING

WARNING

Introduction

Used whenever an action or condition may
potentially cause personal injury or loss of
life. Mechanical damage may also result.

Indicates high voltage or risk of electric
shock. Refer servicing of all areas displaying
either symbol to service personnel.

CFC-FREE CENTRIFUGATION

To ensure minimal environmental impact, no CFCs are used in the
manufacture or operation of Allegra 6 series and Spinchron R

CFC

centrifuges.

RADIO INTERFERENCE

This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environ
ment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
this instruction manual, may cause interference to radio communica
tions. Operation of this equipment in a residential area is likely to
cause interference, in which case the user will be required to correct
the interference at his own expense.

-

-

vii

Introduction

CANADIAN REGULATIONS

RECYCLING LABEL

This equipment does not exceed the Class A limits for radio noise
emissions from digital apparatus as set out in the radio interference
regulations of the Canadian Department of Communications.

Le présent appareil numérique n’émet pas de bruits radioélectriques
dépassant les limites applicables aux appareils numériques de Classe
A prescrites dans le reglement sur le brouillage radioelectrique édicté
par le Ministère des Communications du Canada.

This symbol is required in accordance with the Waste Electrical and
Electronic Equipment (WEEE) Directive of the European Union. The
presence of this marking on the product indicates.

A28219-AA

Note: On the instrument, the
triangle background is yellow
rather than gray.

1) The device was put on the European market after August 13, 2005
and

2) The device is not to be disposed via the municipal waste collection
system of any member state of the European Union.

It is very important that customers understand and follow all laws
regarding the proper decontamination and safe disposal of electrical
equipment. For Beckman Coulter products bearing this label please
contact your dealer or local Beckman Coulter office for details on the
take back program that will facilitate the proper collection, treatment,
recovery, recycling and safe disposal of the device.

viii

CENTRIFUGE FUNCTION AND SPECIFICATIONS

CENTRIFUGE FUNCTION

The Beckman Coulter Allegra 6 centrifuge generates centrifugal
forces required for a wide variety of applications. Together with the
Beckman Coulter rotors designed for use in this centrifuge, the centri
fuge applications include:

• Routine processing such as sample preparations, pelleting, extrac-

tions, purifications, concentrations, phase separations, receptor
binding, and column centrifugations.

1

Description

-

• Processing large numbers of small-volume samples in multiwell

plates for concentrating tissue-culture cells, cloning and replicate
studies, in-vitro cytotoxicity studies, receptor binding, and genetic
engineering experimentation.

• Virus isolation.

• Rapid sedimentation of protein precipitates, large particles, and

cell debris.

• Preparation of subcellular organelles such as mitochondria,

granules, and crude microsomes.

• Binding studies and separation of whole blood.

• Cell isolation.

ALLEGRA 6 AND SPINCHRON MODELS

The Allegra 6 (see Figure 1-1) comes in three models and the
Spinchron in one:

• A nonrefrigerated compact bench model (no Spinchron model)

• A refrigerated compact bench model

• A refrigerated kneewell model (no Spinchron model)

1-1

Description

Allegra 6

Allegra 6R

or

Spinchron R

Allegra 6KR

Figure 1-1. Allegra 6 Centrifuges

1-2

Description

SPECIFICATIONS

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.

Allegra 6R /

Allegra 6 Spinchron R Allegra 6KR

Speed

Set* (in 200-rpm increments). . . . . . . . . 0 to 8000 rpm . . . . . . . . . . . . . . 0 to 8000 rpm . . . . . . . . . . . . . . . . . . 0 to 8000 rpm

Display . . . . . . . . . . . . . . . . . . . . . . ±30 rpm of actual . . . . . . . . . . ±30 rpm of actual . . . . . . . . . . . . . . . ±30 rpm of actual

Set time

Timed run . . . . . . . . . . . . . . . . . . . . . . . . . up to 30 min . . . . . . . . . . . . . . . .up to 30 min . . . . . . . . . . . . . . . . . . . up to 30 min

Continuous run . . . . . . . . . . . . . . . . . . . . . . . . . .HOLD . . . . . . . . . . . . . . . . . . . . HOLD . . . . . . . . . . . . . . . . . . . . . . . . .HOLD

Temperature

Temperature setting

(refrigerated models only) . . . . . . . . . . . not applicable . . . . . . . . . . . . . . . –10 to 40°C . . . . . . . . . . . . . . . . . . . . –10 to 40°C

Normal operating range

(refrigerated models only) . . . . . . . . . . . not applicable . . . . . . . . . . . . . . . . –5 to 25°C . . . . . . . . . . . . . . . . . . . . . –5 to 25°C

Temperature display

(refrigerated models only) . . . . . . . . . . . not applicable . . . . actual chamber temp ±2°C . . . . . . . . actual chamber temp ±2°C

Ambient temperature range . . . . . . . . . . . . 10 to 35°C . . . . . . . . . . . . . . . . . 10 to 35°C . . . . . . . . . . . . . . . . . . . . . 10 to 35°C

Ambient temperature range

for optimum operation . . . . . . . . . . . . . . . . 10 to 25°C . . . . . . . . . . . . . . . . . 10 to 25°C . . . . . . . . . . . . . . . . . . . . . 10 to 25°C

Humidity restrictions <95% (noncondensing) . . . . . . <95% (noncondensing) . . . . . . . . . . . <95% (noncondensing)

Dimensions

Width . . . . . . . . . . . . . . . . . . . . . . . 54.6 cm (21.5 in.) . . . . . . . . . . . . . . 76 cm (30 in.) . . . . . . . . . . . . . . . 54.6 cm (21.5 in.)

Depth (overall) . . . . . . . . . . . . . . . . 66.0 cm (26.0 in.) . . . . . . . . . . . . . . 66 cm (26 in.) . . . . . . . . . . . . . . . 66.0 cm (26.0 in.)

Depth (at base) . . . . . . . . . . . . . . . . 58.0 cm (23.0 in.) . . . . . . . . . . . 58.4 cm (23.0 in.) . . . . . . . . . . . . . . . 58.4 cm (23.0 in.)

Height, door closed. . . . . . . . . . . . . 40.6 cm (16.0 in.) . . . . . . . . . . . 39.4 cm (15.5 in.) . . . . . . . . . . . . . . . 67.3 cm (26.5 in.)

Height, door open . . . . . . . . . . . . . . 96.8 cm (38.1 in.) . . . . . . . . . . . 94.0 cm (37.0 in.) . . . . . . . . . . . . . . 125.7 cm (49.5 in.)

Weight. . . . . . . . . . . . . . . . . . . . . . . 51.7 kg (114.0 lb) . . . . . . . . . . 75.8 kg (167.0 lb) . . . . . . . . . . . . . . . 90.3 kg (199.0 lb)

Ventilation clearances

(sides and rear) . . . . . . . . . . . . . . . . . . 7.6 cm (3.0 in.) . . . . . . . . . . . . . 7.6 cm (3.0 in.) . . . . . . . . . . . . . . . . . 7.6 cm (3.0 in.)

Electrical requirements . . . . . . . . . . . 120 VAC, 8 A, 60 Hz . . . . . . 120 VAC, 12 A, 60 Hz . . . . . . . . . . . . 120 VAC, 12 A, 60 Hz

230 VAC, 4 A, 50 Hz . . . . . . . . 230 VAC, 8 A, 50 Hz . . . . . . . . . . . . . 230 VAC, 8 A, 50 Hz

100 VAC, 10 A, 50/60 Hz . . . . . 200 VAC, 10 A, 50/60 Hz . . . . . . . . . 200 VAC, 10 A, 50/60 Hz

Electrical supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . Class I . . . . . . . . . . . . . . . . . . . . Class I . . . . . . . . . . . . . . . . . . . . . . . . .Class I

Maximum heat dissipation

into room under

steady-state conditions . . . . . . . 3073 Btu/hr (0.9 kW) . . . . . . . 5425 Btu/hr (1.59 kW) . . . . . . . . . . . 5425 Btu/hr (1.59 kW)

Noise level 0.91 m (3 ft)

in front of centrifuge. . . . . . . . . . . . . . . . . . . . <65 dBa . . . . . . . . . . . . . . . . . . <65 dBa . . . . . . . . . . . . . . . . . . . . . . . <65 dBa

Installation (overvoltage) category . . . . . . . . . . . . . . . . . II . . . . . . . . . . . . . . . . . . . . . . . . II . . . . . . . . . . . . . . . . . . . . . . . . . . . . II

Pollution degree . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2† . . . . . . . . . . . . . . . . . . . . . . . 2†. . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

†

* Maximum rated speed (depending on rotor in use) can be obtained only at nominal voltages (120 V for 60 Hz; 230 V for 50 Hz; 100 V

for 50/60 Hz).

† Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected.

1-3

Description

AVAILABLE ROTORS

Refer to the appropriate rotor manuals for complete rotor
descriptions.

Rotor Profile and Description

Rotor Profile and Description

GH-3.8A Horizontal (4 place)

GH-3.8 Horizontal (4 place)

PTS-2000 Horizontal (4 place)

Buckets (r

Carriers (r

Buckets (r

Carriers (r

= 204 mm)

max

= 163 mm)

max

= 204 mm)

max

= 163 mm)

max

Sector base, 100-mm tube
(r

= 170 mm)

max

Sector base, 75-mm tube
(r

= 160 mm)

max

Tube base, 16-mm tube
(r

= 176 mm)

max

Tube base, 13-mm tube
(r

= 176 mm)

max

Micro Plus carriers
(r

= 163 mm)

max

Max

RPM

3750

3250

3750

3250

3250

3250

3250

3250

3250

Max RCF

(× g)

3210

1924

3210

1924

2010

1890

2080

2080

1920

Max

Capacity

4 × 750 mL

4 × 192 mL

4 × 750 mL

4 × 192 mL

7 × 10 mL

(each carrier)

7 × 7 mL

(each carrier)

20 × 10 mL

(each carrier)

20 × 7 mL

(each carrier)

4 × 192 mL

Rotor Manual

Number

GS6-TB-017

GS6-TB-003

SP-TB-008

GH-4.7 Horizontal (4 place)

(r

1-4

= 182 mm) 4730 4560 4 × 250 mL

max

GS-TB-024

Description

Rotor Profile and Description

Rotor Profile and Description

GA-6 Fixed Angle, 35° (6 place)

SAFETY FEATURES

r

= 137 mm

max

Max

RPM

6100 5710 6 × 250 mL

Max RCF

(× g)

Max

Capacity

Rotor Manual

Number

GS-TB-023

The centrifuge has been designed and tested to operate safely indoors
at altitudes up to 2 000 m (6 562 ft). Safety features include the
following.

• The door has an electromechanical door-locking mechanism and a

secondary (manual) lock to prevent operator contact with spinning
rotors. When the door is closed it locks automatically. It can be
unlocked only by pressing the DOOR switch to OPEN, and opened
only when the power is on, the rotor is at rest, and the manual lock
is in the UNLOCK position. Two independent monitoring systems
prevent the door from opening if the rotor is spinning.

• An imbalance detector monitors the rotor during the run, causing

automatic shutdown if rotor loads are severely out of balance. At
low speeds, an incorrectly loaded rotor can cause imbalance. Rotor
instability can also occur if the centrifuge is moved while running,
or if it is not resting on a level surface.

• An anchoring system is provided to secure the centrifuge in place.

The anchoring system (bolts that anchor a benchtop centrifuge to
the laboratory bench; a stationary tray to roll a kneewell centrifuge
into for operation) is designed to minimize the possibility of injury
or damage that could result from centrifuge movement in the event
of a major rotor mishap.

1-5

Description

NAME RATING PLATE

CHASSIS

HOUSING

The name rating plate is affixed to the rear of the centrifuge. Check
that the line voltage agrees with the voltage listed on this name rating
plate before connecting the centrifuge. Always mention the serial
number and the model number shown when corresponding with
Beckman Coulter regarding your centrifuge (the serial number is also
on the inside of the door).

The centrifuge housing is made of sheet steel over a steel chassis and
is finished with acrylic baking enamel. The control panel overlay is
made of coated polycarbonate. Polyvinyl chloride and polyester foam
panels line the interior walls of the centrifuge housing for sound and
temperature insulation.

DOOR

ROTOR CHAMBER

The structural steel door has a strobe port for speed verification.
In nonrefrigerated models, the door has two filtered air-intake
ports for rotor cooling. An electromechanical door lock system,
together with a

manual lock, prevents run initiation unless the door
is closed and latched. When a run is in progress, the door locks
automatically and can not be opened unless the power is on, the rotor
is virtually stopped (spinning less than 40 rpm), and the manual lock
is in the UNLOCK position. If there is a power failure, the door
lock can be manually tripped for sample recovery (see Section 4,

TROUBLESHOOTING).

The aluminum rotor chamber (Figure 1-2) is coated with a chemical­resistant epoxy finish. The drive shaft and neoprene rubber boot
surrounding the drive shaft are visible in the chamber bottom. A
neoprene gasket around the chamber top assures sealing. (Centrifuge
gaskets have not been designed as bioseals for aerosol containment.)
The thermistor is also visible in refrigerated models.

1-6

Description

Drive Shaft

Neoprene Boot

Neoprene Gasket

Exhaust Holes

Thermistor

Refrigerated Nonrefrigerated

Figure 1-2. Interior View of the Centrifuge

Drive Shaft Neoprene Boot

Neoprene Gasket

Nonrefrigerated models only—During centrifugation, the rotor move­ment draws air through the filtered air-intake ports in the door into the
chamber bowl, where it circulates around the rotor. Holes in the side
of the bowl allow air to flow out to the exhaust ports beneath the
centrifuge. This air flow provides cooling for the rotor.

DRIVE

The direct-drive motor has permanently lubricated, sealed bearings.
A

tie-down screw (or rotor knob) secures the rotor to the drive shaft.
The resilient suspension ensures that loads will not be disturbed by
vibration, and prevents damage to the drive shaft if an imbalance
occurs during centrifugation.

REFRIGERATION SYSTEM (Refrigerated Models Only)

A refrigeration compressor circulates CFC-free refrigerant through
copper tubing wrapped around the chamber bowl, which is insulated
to increase refrigeration efficiency. A thermistor regulates the
compressor to maintain chamber temperature to ±2°C of set
temperature.

1-7

Description

CONTROLS AND INDICATORS

CONTROL PANEL

NOTE

➠

To avoid chamber icing, refrigeration is off
when the door is open. The centrifuge door must
be closed and locked for the refrigeration system
to begin operating.

Mounted at an angle on the centrifuge front for easy visibility

access, the control panel (Figure 1-3, nonrefrigerated model;

and

1-4, refrigerated models) comprises indicators, control knobs,

Figure
and switches.

POWER This two-position rocker switch, labeled I (ON) and O (OFF),

controls electrical power to the centrifuge. The switch lights up
when

the power is on.

DOOR When pressed to OPEN, this toggle switch releases the latch mecha-

nism and allows the door to be opened (the rotor must be stopped, the
power must be on, and the manual lock in UNLOCK position).

Figure 1-3. The Control Panel (Nonrefrigerated Model)

1-8

Description

Figure 1-4. The Control Panel (Refrigerated Models)

BRAKE This switch is used to select the brake mode used during deceleration.

Refer to the appropriate rotor manual for acceleration and decelera

­tion times for available rotors under various conditions. The three
positions of the switch provide:

• HIGH position activates high dynamic braking that starts immedi-

ately after the timer turns the motor off. When rotor speed reaches
50 rpm, all braking stops and the rotor coasts to a stop.

• OFF removes braking entirely and allows the rotor to coast to a

stop from set speed.

• LOW position activates low dynamic braking down to 50 rpm, and

is used for slower deceleration to avoid remixing or resuspension
of the sediment.

TIME This variable-setting knob may be set for timed runs or longer-

duration continuous runs. Either type of run can be stopped at any
time by switching the timer to OFF.

•Timed runs — the knob may be set for any run time up to

minutes in 1-minute increments.

30

• Continuous runs — with the knob set to HOLD, the centrifuge will

run until turned off.

SPEED The variable-setting knob is used to set the drive speed, in 200-rpm

increments, up to 8000 rpm. For precise speed adjustments, the
ACCU-SET button can be pressed as the speed knob is turned until
the required speed appears on the RPM display. The centrifuge will
accelerate to the set speed and maintain that speed for the duration of
the run.1 ACCU-SET can be adjusted at any time during a run.

1

Actual speed may fluctuate due to line voltage variation.

1-9

Description

RPM When centrifuge power is turned on, the display shows “0000.”

During the run the display indicates actual rotor speed to the nearest
10-rpm increment. If the run is shut down prematurely due to one

four conditions (see Section 4, TROUBLESHOOTING), the

of
diagnostic message “8880” will be displayed.

TEMPERATURE
(refrigerated models only)

°C
(refrigerated models only)

10 20

-10

0

CENTIGRADE

°

30

40

This knob is used to set the operating temperature between –10 and
+40°C in 1°C increments. (Recommended operating temperature
range is from –5 to +25°C.) A thermistor in the chamber regulates

compressor to maintain chamber temperature ±2°C of the set

the
temperature.2

NOTE

➠

Longer continuous runs are required to achieve
temperatures higher than 25°C.

Displays the temperature (±2°C) within the chamber bowl.

2

Temperature control is referenced for 25°C ambient; temperature performance at higher ambient will depend on the rotor in use and
environmental conditions.

1-10

!

WARNING

2

Installation Requirements

This centrifuge weighs 51.7 kg/114.0 lb
(nonrefrigerated benchtop), 75.8 kg/167.0 lb
(refrigerated benchtop), or 90.3 kg/199.0 lb
(refrigerated kneewell). DO NOT attempt to
lift or move it without assistance from
another person.

!

WARNING

Do not place the centrifuge near areas
containing flammable reagents or
combustible fluids. Vapors from these
materials could enter the centrifuge’s air
system and be ignited. Never bring any
flammable substances within the 30-cm (1-ft)
area surrounding the centrifuge. Maintain a
clearance envelope of 7.6 cm (3 in.) around an
operating centrifuge. No persons should be
within this clearance envelope while the
centrifuge is operating.

SPACE AND LOCATION REQUIREMENTS

Carefully remove the centrifuge and accessories from the shipping
container. Save the container and packing materials for possible
future relocation or storage of the centrifuge.

2-1

Installation Requirements

• Select a location away from heat-producing laboratory equipment,

with sufficient ventilation to allow for heat dissipation.

• Position the centrifuge on a level surface (a sturdy table or labora-

tory bench for benchtop models) that can support the weight of the
centrifuge (refer to

SPECIFICATIONS in Section 1) and resist

vibration.

• Allow a 7.6-cm (3-in.) clearance at each side and the back of the

centrifuge to ensure sufficient air circulation. Dimensions are
shown in Figure 2-1. The centrifuge must have adequate air venti
lation to ensure compliance to local requirements for vapors
produced during operation.

• Do not operate the centrifuge in ambient temperatures exceeding

35°C (95°F). Relative humidity should not exceed 95%
(noncondensing).

Use the appropriate anti-rotation kit to secure the centrifuge to the
working surface. The anti-rotation kit is designed to minimize the
possibility of injury or damage that could result from centrifuge
movement in the event of a rotor mishap.

-

• The anchoring kit for a benchtop centrifuge contains bolts to

secure the centrifuge to the table or bench. Complete instructions
(SP-TB-007) for installing the anti-rotation kit are included with
the kit, which is shipped with the centrifuge.

• The anchoring kit for a kneewell centrifuge contains a stationary

tray to roll the centrifuge into for operation. Complete instructions
(GS6-TB-007) for installing the anti-rotation kit are included with
the kit, which is shipped with the centrifuge.

ELECTRICAL REQUIREMENTS

Allegra 6 Spinchron R Allegra 6KR

120 VAC, 8 A, 60 Hz 120 VAC, 12 A, 60 Hz 120 VAC, 12 A, 60 Hz
230 VAC, 4 A, 50 Hz 230 VAC, 8 A, 50 Hz 230 VAC, 8 A, 50 Hz
100 VAC, 10 A, 50/60 Hz 200 VAC, 10 A, 50/60 Hz 200 VAC, 10 A, 50/60 Hz

A 1.8-m (6-ft) power cord with grounded plug is supplied with the
centrifuge. In regions where a different plug is required to meet local
electrical and safety requirements, contact your local Beckman
Coulter representative.

Allegra 6R /

2-2

37.0

94.0

Installation Requirements

37.0

94.0

26.0

66.0

23.0

58.4

Allegra 6R

or

Spinchron R

3.0

7.6

30.0

76.2

15.5

39.4

26.0

66.0

23.0

58.4

3.0

7.6

15.5

39.4

3.0

7.6

21.5

54.6

3.0

7.6

Allegra 6

in.

cm

49.5

125.7

Allegra 6KR

26.0

66.0

23.0

58.4

3.0

7.6

Figure 2-1. Centrifuge Dimensions

26.5

67.3

3.0

7.6

21.5

54.6

2-3

Installation Requirements

Make sure the voltage imprinted on the name rating plate affixed to
the back of the centrifuge agrees with the line voltage of the outlet
used. With the POWER switch in the OFF (O) position, plug in both
ends of the centrifuge power cord. If there is any question about
voltage, have a qualified service person measure it under load while
the drive is operating.

To reduce the risk of electrical shock, this equipment uses a
three-wire electrical cord and plug to connect the centrifuge to
earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly

wired and earth-grounded.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding

type of multiple-outlet receptacle strip.

To ensure safety, the centrifuge should be wired to a remote emer­gency switch (preferably outside the room where the centrifuge is
housed, or adjacent to the exit from that room), in order to disconnect
the centrifuge from the main power source in case of a malfunction.

TEST RUN

NOTE

➠

During transport between areas with varying
temperatures, condensation may occur inside the
centrifuge. Allow sufficient drying time before
running the centrifuge.

We recommend that you make a test run to ensure that the centrifuge
is in proper operating condition after shipment. See Section 3 for
instructions on operating the centrifuge.

After completing the test run, return the preaddressed warranty card
included with this literature. This will validate the centrifuge
warranty and ensure your receipt of further information regarding
new accessories and/or modifications as they become available.

2-4

!

WARNING

Operation

The centrifuge must not be used in the
vicinity of flammable liquids or vapors,
and

such materials should not be run in

centrifuge. Never bring any flammable

the
substances within the 30-cm (1-ft) area
surrounding the centrifuge. During operation
you should not come within the 7.6-cm (3-in.)
clearance envelope except to adjust centrifuge
controls, if necessary. Do not lean on the
centrifuge or place items on the centrifuge
while it is operating.

3

RUN PROCEDURES

The following detailed operating procedures describe operation of the
centrifuge. Refer to the appropriate rotor manual for instructions on
preparing the rotor for centrifugation.

3-1

Operation

!

WARNING

Handle body fluids with care because they
can transmit disease. No known test offers
complete assurance that they are free of
micro-organism. Some of the most virulent
Hepatitis (B and C) and HIV (I–V) viruses,
atypical mycobacteria, and certain systemic

— further emphasize the need for

fungi
aerosol protection.

Handle other infectious samples according to
good laboratory procedures and
prevent spread of disease. Because spills may
generate aerosols, observe proper safety
precautions for aerosol containment. Use only
the appropriate rotors and adapters. Do not
run toxic, pathogenic, or radioactive
materials in this centrifuge without taking
appropriate safety precautions. Biosafe hoods
should be used when Risk Group II materials
(as identified in the World Health Organiza
tion Laboratory Biosafety Manual) are
handled; materials of a higher group require
more than one level of protection.

methods to

—

-

3-2

PREPARATION AND LOADING

Action Result

1. Ensure that the power cord is
plugged into a wall recep­tacle that provides the correct
voltage.

2. Press the POWER switch to
ON (I).

Power is available to the centrifuge.

Power is applied to the system.

Action Result

3. Move the manual lock lever
to the left (UNLOCK).

UNLOCK LOCK

Operation

4. Press DOOR to OPEN posi­tion, then lift the door up.

5. Install the rotor or rotor yoke.
Be sure the base is seated on
the drive shaft, with the pin
in the rotor groove.

Groove

Drive Shaft

Pin

Door remains open.

!

CAUTION

Never drop the rotor onto the centrifuge drive
shaft. The shaft can be damaged if the rotor is
dropped onto it or forced sideways.

The rotor is ready to load. (You can load fixed angle rotors either
before or after installing the rotor in the centrifuge.)

6. Load the rotor according to
the rotor manual. Always run
the rotor with a balanced
load; fill all positions on a
swinging bucket rotor yoke
with buckets and/or carriers.

!

WARNING

Do not substitute a metal fastener for the
plastic tie-down nut supplied with the rotor.
If

a tie-down nut comes off the shaft during
centrifugation, the plastic nut furnished will
break apart in the chamber, causing minimal
damage to the centrifuge. A loose metal object
could substantially damage the rotor,
chamber, and lid, and could potentially
escape the chamber into the laboratory,
causing personal injury or property damage.

3-3

Operation

Action Result

7. Firmly fasten the tie-down
nut on the shaft using the
torquing bar (356036).

not overtighten the nut.

Do

8. Close the door. Push firmly
down until you hear a
clicking (latching) sound
indicating that the door is
latched.

9. Move the manual lock lever
to the right (LOCK).

UNLOCK LOCK

The rotor is secured to the shaft.

NOTE

➠

The tie-down nut is in the lid knob on the GA-6
fixed angle rotor. If you are using this rotor,
install the lid and turn the knob to the right
(clockwise) until firmly fastened on the shaft.

STARTING A RUN

Action Result

1. Set run speed.

• Press and hold the

ACCU-SET button.

• Rotate the SPEED dial to

the selected speed.

• Release the ACCU-SET

button.

The selected speed shows on the display.

3-4

Action Result

2. Select the brake position.

3. Set the TEMPERATURE
knob to the required run
temperature (refrigerated
models only).

Operation

4. Turn the TIME control to the
required run time.

As the run begins, acceleration control protects delicate gradients;
when the rotor reaches about 200 rpm, full acceleration to set speed
occurs.

• The digital RPM display indicates the rotor speed.

• The TIME control knob shows the time remaining in the run.

• The °C display indicates the chamber temperature (refrigerated
models only).

10 20

➠

NOTE

-10

0

CENTIGRADE

°

30

40

Turn the knob to the right (clockwise) for time
setting or to the left (counterclockwise) to
HOLD.

!

CAUTION

Do not attempt to override the door interlock
system while the rotor is spinning. Do not lift or
move the centrifuge while the rotor is spinning.

3-5

Operation

STOPPING A RUN

A timed run ends automatically when the TIME control counts down
to zero. To end a run in progress for any reason:

Action Result

1. Turn the TIME control knob
to OFF.

2. After the rotor stops spin­ning, move the manual lock
lever to the left (UNLOCK)

UNLOCK LOCK

3. Press the DOOR switch to
OPEN.

The door interlock system will keep the door latched until the rotor
has virtually stopped (spinning less than 40 rpm).

NOTE

➠

Do not stop a run by turning the SPEED knob

0. This will disengage the brake and the rotor

to
will coast to a stop, which could take a long
time. Always use the TIME knob to stop a run.

The door interlock system will release the latch and allow the door

be opened when the rotor speed is 40 rpm or less. However, for

to
operator safety, do not open the door until the rotor has come to a

.

complete stop.

!

CAUTION

If disassembly reveals evidence of leakage, you
should assume that some fluid escaped the rotor.
Apply appropriate decontamination procedures
to the centrifuge and accessories.

3-6

After completing a run, unload the rotor as described in the appro­priate rotor manual. It is not necessary to remove the rotor from the
centrifuge between runs unless the rotor or chamber bowl needs to be
cleaned. (If the rotor is left in the centrifuge between runs, make sure
the rotor is seated on the drive shaft and the tie-down nut is tight
before each run.)

NOTE

➠

Accumulations of sample, dust, and/or glass
particles from broken sample tubes can ruin
rotor pins and cause rotor vibrations. To prevent
these accumulations, remove the rotor and clean
the centrifuge bowl often. See
Section 5.

CLEANING in

➠

4

Troubleshooting

NOTE

It is your responsibility to decontaminate the
centrifuge, as well as any rotors and accessories,
before requesting service by Beckman Coulter
representatives.

SHUTDOWN/NO-START DIAGNOSTICS

If one of the conditions described in Table 4-1 shuts the centrifuge
down prematurely, the rotor will decelerate to a stop and the RPM
display will show “8880.” Refer to Table 4-1 to determine the nature
of the problem and recommended corrective actions. Possible causes
for each problem are listed in the probable order of occurrence.
Perform the recommended corrective action in sequence, as listed.

the problem persists, call Beckman Coulter Field Service.

If

To help diagnose and correct the problem, try to provide as much
information as possible:

• Note the operating situation when the error occurred (such as
speed or load type).

• Note any unusual environmental and/or operating conditions (such
as ambient temperature or voltage fluctuations).

• Add any other information that may be helpful.

4-1

Troubleshooting

OTHER POSSIBLE PROBLEMS

Probable Cause Recommended Action

Possible malfunctions not indicated by diagnostic messages are
described in Table 4-2, along with probable causes and corrective
actions required. Possible causes for each problem are listed in the
probable order of occurrence. If you are unable to correct the
problem, call Beckman Coulter Field Service.

Table 4-1. Shutdown/No-Start Diagnostics

Probable Cause

Imbalance detector
tripped

Thermal cutoff switch
activated

1. Turn the TIME knob to OFF and press the POWER switch to OFF (O).

2. Check to be sure the rotor is in good condition and is loaded symmetrically
around the center of rotation, with containers of equal weight and density
opposite each other.

NOTE

If you are using a swinging bucket rotor, fill all yoke positions with buckets and/
or carriers. Always run the rotor with a balanced load (refer to the applicable
rotor manual for complete loading information).

3. Be sure the rubber boot is undamaged and is seated correctly over the dust cover.

4. After a 30-second delay, press the POWER switch to ON (I) to reset the imbalance
detector circuit. You should be able to restart the run normally.

NOTE

It is possible to trip the imbalance detector switch during rotor installation or
loading. Performing these actions with the power off will avoid the problem. If
the imbalance switch is tripped, however, turn power to the centrifuge off, then
back on to reset the circuit.

1. Turn the TIME knob to OFF and press the POWER switch to OFF (O).

2. Check that ambient temperature is within the limits shown under SPECIFICATIONS
in Section 1.

3. Check for air inlet obstruction and dirty air-inlet filters. Clean as required (see
Section 5, Care and Maintenance).

4. After the motor has cooled (approximately 15 minutes with the door open), press the
POWER switch to ON (I) to reset the thermal protector circuit. If the problem per
sists, call Beckman Coulter Field Service.

-

Chamber over­temperature
(refrigerated models
only)

Tachometer signal loss Call Beckman Coulter Field Service.

1. Turn the TIME knob to OFF and press the POWER switch to OFF (O).

2. Check that ambient temperature is within the limits shown under SPECIFICATIONS
in Section 1.

3. Check for air inlet obstruction and dirty air-inlet filter. Clean as required (see
Section 5, Care and Maintenance).

4. Check chamber temperature; if it is overtemperature, call Beckman Coulter Field
Service.

4-2

Table 4-2. Troubleshooting Chart

Problem Probable Cause Recommended Action

Problem

Troubleshooting

Centrifuge does
not start

Rotor cannot
achieve set
speed

Set temperature
cannot be
achieved
(refrigerated
models only)

Temperature
cannot be
maintained
(refrigerated
models only)

Door will not
open

Power not on Plug in power cord; press POWER switch to ON (I). Verify

proper power to wall outlet.

Line voltage below rating Measure line voltage while the centrifuge is operating.

Imbalance detector not reset Press POWER switch to OFF (O) position; after 30 seconds,

turn it back ON (I).

Thermal protector not reset after
rotor cools

Electrical failure Check connections; call Beckman Coulter Field Service.

Motor bearings or commutator worn Motor needs replacing; call Beckman Coulter Field Service.

Motor failure Call Beckman Coulter Field Service.

Ambient temperature too high Reduce ambient temperature.

Run too short to achieve temperature Precool rotor; run for a longer time.

Temperature knob needs adjustment Adjust knob (see Section 5, Care and Maintenance).

Dirty filter Clean filter (see Section 5, Care and Maintenance).

Rotor hot Precool rotor.

Excess moisture in chamber Wipe out moisture before each run.

Tear or break in chamber gasket Call Beckman Coulter Field Service.

Refrigeration or electronic problem Call Beckman Coulter Field Service.

Ambient temperature too high Reduce ambient temperature.

Rotor speed higher than rated Reduce rotor speed.

Dirty filter Clean filter (see Section 5, Care and Maintenance).

Refrigeration or electronic problem Call Beckman Coulter Field Service.

Power not on Plug in power cord; press POWER switch to ON (I).

Rotor spinning over 40 rpm Wait until rotor stops.

Secondary latch lever in LOCK
position

Press POWER switch to OFF (O) position; after 60 seconds,
turn it back ON (I).

Move latch lever to UNLOCK.

Latch stuck See EMERGENCY ACCESS, below.

Rotor speed
shows “0000”
and rotor is
spinning

Rotor speed
display is blank

Chamber icing
(refrigerated
models only)

Display malfunction Call Beckman Coulter Field Service.

Power not on Plug in power cord; press POWER switch to ON (I).

Fuse blown Call Beckman Coulter Field Service.

Excess humidity in chamber Wipe out moisture before each run.

Condensation build-up between runs Turn refrigeration off between runs by one of the following:

• Leave door open between runs.

• Turn TEMPERATURE knob to a setting higher than ambient
temperature.

• Turn centrifuge power off.

4-3

Troubleshooting

EMERGENCY ACCESS

If the facility power fails, the centrifuge will resume operation when
power is restored and the rotor will return to set speed. In the event
of

an extended power failure, it may be necessary to trip the door­locking mechanism manually to remove the rotor and retrieve your
sample.

!

WARNING

The following procedure may expose the
operator to the possibility of contact with

spinning rotor. Turn the power off and

a
disconnect the centrifuge from the main
power source before proceeding. Never
attempt to override the door interlock
system while the rotor is spinning.

Action Result

1. Press the POWER switch
to OFF (O) and unplug
the power cord from the
power source.

2. Make sure that the rotor
is not spinning.

3. Move the secondary latch
lever to UNLOCK.

4. Insert a 4-mm (5/32-inch)
internal hex wrench into
the access opening in the
centrifuge side panel (see
Figure

4-1).

No indicators are lit.

No sound or vibration comes from the centrifuge.

4-4

Action Result

Troubleshooting

Secondary (manual)
Lock Lever

Figure 4-1. Emergency Access

5. Turn the hex wrench to
the right (clockwise) until
you hear a “click” or the
door opens.

6. Open the door and remove
the sample.

The latch releases and the door can be opened.

If the rotor is still spinning, close the door and wait until it stops
before attempting to remove it.

!

WARNING

Never try to slow or stop the rotor by hand.

4-5

Care and Maintenance

For maintenance not covered in this manual, contact Beckman
Coulter Field Service (1-800-742-2345 for Allegra 6 models or
1-800-854-3633 for Spinchron models; customers outside the United
States should contact their local Beckman Coulter representative).
Refer to the applicable rotor manual and Chemical Resistances
(publication IN-175) for instructions on the care of rotors and rotor
accessories.

5

!

➠

WARNING

Any maintenance procedure or servicing of
this equipment that requires removal of any
covers can expose parts which involve the risk
of electric shock or personal injury. Make
sure that the POWER switch is OFF (O) and
the centrifuge is disconnected from the main
power source, and refer such servicing to
qualified service personnel.

Do not use alcohol or other flammable
substances in or near operating centrifuges.

NOTE

It is your responsibility to decontaminate the
centrifuge, as well as any rotors and accessories,
before requesting service by Beckman Coulter
Field Service.

5-1

Care and Maintenance

GENERAL MAINTENANCE

Perform the following procedures regularly to ensure satisfactory
performance and long service life of the centrifuge.

• Regularly inspect the interior of the rotor chamber for accumula­tions of sample, dust, or glass particles from broken sample tubes.
Clean as required (see
can result in rotor vibrations.

• Regularly check the air intake and exhaust vents for obstructions.
Keep vents clear and clean.

• To prevent the rotor from sticking, lubricate the drive shaft with
Spinkote (306812) at least once a month (depending on usage),
and after each cleaning.

NOTE

➠

Before using any cleaning or decontamination
methods except those recommended by the
manufacture, users should check with the
manufacturer that the proposed method will not
damage the equipment.

CLEANING, below), as these accumulations

5-2

CLEANING

Frequent cleaning will ensure proper operation and prolong the life

the centrifuge. Always clean up spills when they occur to prevent

of
corrosives or contaminants from drying on component surfaces.

• To prevent accumulations of sample and/or dust, keep the interior
of the rotor chamber clean and dry by frequent wiping with a cloth
or paper towel. Remove the rotor and wash the bowl often, using a
mild detergent such as Beckman Solution 555™ (339555). Dilute
the detergent 10 to 1 with water. Rinse thoroughly and dry
completely. Spray the centrifuge bowl with antistatic solution and
wipe it clean. (Anti-static wipes are also available.)

• Clean the drive shaft, shaft cavity, threads, and the tie-down screw
at least once a week using a mild detergent such as Solution 555
and a soft brush. Dilute the detergent 10 to 1 with water. Rinse
thoroughly and dry completely. Lubricate the drive shaft with
Spinkote (306812) after cleaning.

Care and Maintenance

• Clean the centrifuge case and door by wiping with a cloth damp­ened with Solution 555. Do not use acetone or other solvents.

Air Intake
Filters

• Use a soft brush or vacuum to clean the air intake vent filter when
dust accumulates.

• (Nonrefrigerated models only) Remove the air-intake filters and
inspect for dust and dirt. Wash them using a mild soap or detergent
such as Solution 555. Rinse well and squeeze excess water out,
then air-dry before reinserting.

DECONTAMINATION

If the centrifuge and/or accessories are contaminated with radioactive
or pathogenic solutions, perform appropriate decontamination proce
dures. Refer to Chemical Resistances to be sure the decontamination
method will not damage any part of the centrifuge.

STERILIZATION AND DISINFECTION

1

Ethanol (70%)
Resistances for more information regarding chemical resistance of
centrifuge and accessory materials.

may be used on the centrifuge surface. See Chemical

-

While Beckman Coulter has tested these methods and found that they
do not damage the centrifuge, no guarantee of sterility or disinfection
is expressed or implied. When sterilization or disinfection is a
concern, consult your laboratory safety officer regarding proper
methods to

TUBE BREAKAGE

If a glass tube breaks, and all the glass is not contained in the bucket
or rotor, thoroughly clean the interior of the chamber bowl.

!

CAUTION

1

Flammability hazard. Do not use in or near operating centrifuges.

use.

Be careful when examining or cleaning the
gasket or chamber, as sharp glass fragments
may be embedded in their surfaces.

5-3

Care and Maintenance

SPEED CALIBRATION

SPEED VERIFICATION

• Examine the gasket to make sure that it is free of glass particles.
Carefully remove any glass particles that may remain. If glass is
under the gasket, call Beckman Coulter Field Service to replace
the gasket.

• Carefully wipe away any glass particles that remain in the bowl.

To compare the actual rotor speed with the set speed, proceed as
follows, using a digital strobe.

Action Result

1. Install a rotor.

2. Secure the rotor tie-down nut
onto the drive shaft.

3. Place a strip of reflective tape
on half of the tie-down nut.

4. Shut the centrifuge door.
Move the secondary latch
lever to LOCK.

5. Set the SPEED knob to the
selected speed.

6. Turn the TIME knob to
HOLD.

The centrifuge will start.

Reflective
Tape

7. When the rotor reaches set
speed, use a calibrated strobe
at the strobe port to verify the
actual rotor speed is within
±30 rpm of the display.

5-4

If there is a discrepancy, check the calibration of your strobe. If you
cannot determine the problem, call Beckman Coulter Field Service.

SPEED KNOB ADJUSTMENT

If set and indicated speed are not in agreement, the SPEED knob may
need adjustment.

Action Result

1. Install a rotor.

2. Set the SPEED knob to
3000

rpm.

Care and Maintenance

3. Check that the door is shut
and securely latched. Move
the secondary latch lever to
LOCK.

4. Turn the TIME knob to
HOLD.

5. When the rotor speed
stabilizes, adjust the knob
until the displayed speed is

rpm.

3000

6. Loosen the two setscrews on
the speed knob (one at each
end of the knob). Rotate the
knob on the shaft until it
indicates 3000 rpm. Tighten
the setscrews.

The centrifuge will start.

5-5

Care and Maintenance

TEMPERATURE KNOB ADJUSTMENT (Refrigerated Models Only)

Action Result

1. Turn the centrifuge
power off.

2. Tap lightly on the °C meter
until the needle is centered
at 15°C.

If set and indicated temperature are not in agreement, the TEMPERA­TURE knob may need adjustment.

-10

10 20

0

CENTIGRADE

°

30

40

3. Turn the power back on and
set the TEMPERATURE
control knob to 40°C.

4. Check that the door is
completely closed and the
manual latch lever is in
LOCK position.

5. Let the centrifuge run for a
short time until the tempera
ture needle stabilizes.

6. Rotate the knob slowly
toward a colder setting (such
as 20°C) until the compres

­sor turns on. Loosen the two
setscrews in the knob (one on
each end of the knob) and set
the knob to the indicated
temperature.

-

-10

10 20

0

CENTIGRADE

°

30

40

5-6

Action Result

7. Repeat step 6 until set and
indicated temperatures are
within one degree of each
other.

STORAGE AND TRANSPORT

STORAGE

Before storing a centrifuge for an extended period, return it to

original shipping container to protect it from dust and dirt.

the
Temperature and humidity conditions for storage should meet the
environmental requirements described under SPECIFICATIONS, in
Section 1.

Care and Maintenance

RETURNING A CENTRIFUGE

RGA

Before returning a centrifuge or accessory for any reason, prior
permission (a Returned Goods Authorization form) must be obtained
from Beckman Coulter, Inc. Contact your local Beckman Coulter
office to obtain the RGA form and for packaging and shipping
instructions.

To protect our personnel, it is the customer’s responsibility to ensure
that all parts are free from pathogens and/or radioactivity. Steriliza

-

tion and decontamination must be done before returning the parts.

All parts must be accompanied by a signed note, plainly visible on the
outside of the box, stating that they are safe to handle and that they
are not contaminated with pathogens or radioactivity. Failure to

attach this notification will result in return or disposal of the
items without review of the reported problem.

5-7

Care and Maintenance

SUPPLY LIST

Contact Beckman Coulter Sales (1-800-742-2345 in the United
States; worldwide offices are listed on the back cover of this manual)
for information about ordering parts and supplies. See the Beckman
Coulter Benchtop Rotors, Tubes & Accessories catalog (BR-9742,
available at www.beckmancoulter.com) for detailed information on
ordering rotors, tubes, and accessories. For your convenience, a
partial list is given below. Refer to the rotor manual for materials
and

supplies needed for rotors.

NOTE

➠

Publications referenced in this manual can be
obtained by calling Beckman Coulter at 1-800­742-2345 in the United States, or by contacting
your local Beckman Coulter office.

REPLACEMENT PARTS

SUPPLIES

Power cord (60-Hz). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 961919

Power cord (50-Hz). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355810

Neoprene boot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 358009

Front panel knob . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 974672

Rotor tie-down nut . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 356035

Rotor tie-down nut for GS-3.8A rotor. . . . . . . . . . . . . . . . . . . . . . . . . 366642

Tie-down nut O-ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 927571

Torquing bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 356036

Anti-Seize (1 1/2 oz) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 961660

Spinkote lubricant (2 oz). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 306812

Silicone vacuum grease (1 oz) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335148

Beckman Solution 555 (1 qt) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339555

5-8

ALLEGRA 6 AND SPINCHRON CENTRIFUGE WARRANTY

Subject to the exceptions and upon the conditions specified
below and the warranty clause of the Beckman Coulter, Inc.
terms and conditions in effect at the time of sale, Beckman
Coulter agrees to correct either by repair or, at its election, by
replacement, any defects of material or workmanship which
develop within one (1) year after delivery of an Allegra 6
Series or Spinchron centrifuge (the product), to the original
buyer by Beckman Coulter or by an authorized representative,
provided that investigation and factory inspection by Beckman
Coulter discloses that such defect developed under normal and
proper use.

Some components and accessories by their nature are not
intended to and will not function for as long as one (1) year. A
complete list of such components or accessories is maintained
at the factory and at each Beckman Coulter District Sales
Office. The lists applicable to the products sold hereunder
shall be deemed to be part of this warranty. If any such compo
nent or accessory fails to give reasonable service for a reason­able period of time, Beckman Coulter will repair or, at its
election, replace such component or accessory. What consti
tutes either reasonable service and a reasonable period of time
shall be determined solely by Beckman Coulter.

-

-

REPLACEMENT

Any product claimed to be defective must, if requested by
Beckman Coulter, be returned to the factory, transportation

charges prepaid, and will be returned to Buyer with the trans­portation charges collect unless the product is found to be
defective, in which case Beckman Coulter will pay all trans
portation charges.

-

CONDITIONS

Beckman Coulter shall be released from all obligations under
all warranties, either expressed or implied, if the product(s)
covered hereby are repaired or modified by persons other than
its own authorized service personnel, unless such repair in the
sole opinion of Beckman Coulter is minor, or unless such
modification is merely the installation of a new Beckman
Coulter plug-in component for such product(s).

DISCLAIMER

IT IS EXPRESSLY AGREED THAT THE ABOVE WAR­RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF
FITNESS AND OF THE WARRANTY OF MERCHANT
ABILITY AND THAT NEITHER BECKMAN COULTER,
INC., NOR ITS SUPPLIERS SHALL HAVE ANY
LIABILITY FOR SPECIAL OR CONSEQUENTIAL
DAMAGES OF ANY KIND WHATSOEVER ARISING
OUT OF THE MANUFACTURE, USE, SALE, HAN­DLING, REPAIR, MAINTENANCE, OR REPLACEMENT
OF THE PRODUCT.

-

Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821

Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com

©2009 Beckman Coulter, Inc.

All rights reserved