Beckman Coulter Optima XE User Manual

User Guide

Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821

Optima XE Series

Preparative Ultracentrifuges
For non-In Vitro Diagnostic Use

PN B07711AA
May 2011

Optima XE
Ultracentrifuge
User Guide

PN B07711AA (May 2011)

© 2011 Beckman Coulter, Inc.
All Rights Reserved

Find us on the World Wide Web at:

www.beckmancoulter.com

Beckman Coulter Ireland, Inc.
Mervue Business Park, Mervue Galway, Ireland 353 91 774068

Beckman Coulter do Brasil Com e Imp de Prod de Lab Ltda
Estr dos Romeiros, 220 - Galpao G3 - Km 38.5
06501-001 - Sao Paulo - SP - Brasil
CNPJ: 42.160.812/0001-44

製造販売元 : ベックマン･コールター株式会社
東京都江東区有明三丁目5番7号
TOC 有明ウエストタワー

贝克曼库尔特有限公司，
美国加利福尼亚州，Brea 市，S. Kraemer 大街 250 号，
邮编：92821 电话：[phone]

CHAPTER 1: Safety and Notices,1-1

Alerts for Danger, Warning, Caution, Important, and Note,1-1

Safety Notices,1-2

Information,1-2
Installation,1-2
Replacement Parts,1-3
Service,1-3
Power Supply,1-3
Fuse Replacement,1-3
Mechanical Safety,1-4
Chemical and Biological Safety,1-4
Precautions with Liquids,1-5
Volatile Liquids,1-5

Contents

Certification,1-5

Scope of Manual,1-5

CFC-Free Centrifugation,1-6

Radio Interference,1-6

United States FCC Certification,1-6
Canadian Department of Communications Certification,1-6
Industry Canada,1-6

Software Copyright,1-7

Summary of Instrument Labels,1-7

Caution Symbol,1-7
RoHS Caution,1-7
Universal Serial Bus (USB),1-8
Ethernet,1-8
Recycling Label,1-8
Multiple Compliance,1-9
CAUTION Static Sensitive Area,1-9
DANGER High Voltage,1-9
Protective Ground,1-10
Rotor Rotation,1-10

Symbols,1-11

iii

Contents

CHAPTER 2: Description,2-1

For non-In Vitro Diagnostic Use,2-1

Touch Screen,2-1

Name Rating Plate,2-1

Rotor Chamber,2-2

Vacuum System,2-2

Temperature Sensing and Control,2-2

Drive,2-2

Safety Features,2-3

Door,2-3
Barrier Ring,2-3
Imbalance Detector,2-3
Overspeed System,2-3
Dynamic Rotor Inertia Check (DRIC),2-3

Specifications,2-4

Control Features,2-4
Physical Data,2-5
Audible Sounds,2-6

Available Rotors,2-7

CHAPTER 3: The Touch Screen Interface,3-1

Areas on the Screen,3-1

Header Bar,3-2

Home Page Button,3-2
Menu Button,3-2
Status Display,3-3
Help Button,3-4

Footer Bar,3-4

Start Button,3-4
Stop Button,3-4
Footer Bar on the Home Page,3-5
Footer Bar on Other Pages,3-5
Vacuum Display/Button,3-5
System Name,3-5
Accel and Decel Display/Buttons,3-6

Page Display Area,3-6

Help Messages,3-6

Item Help,3-6
Global Help,3-7

CHAPTER 4: Operations,4-1

Manual Operation,4-1

Step 1: Start on the Home Page,4-2

iv

Step 2: Set the Speed and Rotor,4-3
Step 3: Set the Time,4-5
Step 4: Set the Temperature,4-6
Step 5: Start the Run,4-7

Zonal and Continuous Flow Operation,4-7

Preparing for the Run,4-8
Starting the Run,4-9
Loading the Sample,4-9
Running the Sample,4-10
Unloading the Sample,4-10
Stopping the Run,4-10
Finishing the Run,4-10

CHAPTER 5: Functional Pages,5-1

Home Page,5-2

w²t Display,5-2
Set Speed Display/Button,5-3
Set Time Display/Button,5-3
Set Temp Display/Button,5-3

Contents

Menu Page,5-4

Set Speed Page,5-5

Set Time Page,5-6

Set Speed Time Page (w²t Mode),5-7

Set Temperature Page,5-8

Set Acceleration/Deceleration Profiles Page,5-9

Example Acceleration and Deceleration Profiles,5-10

Select Rotor and Labware,5-11

References Page,5-12

System Options Page,5-13

Zonal Authorization Page,5-14

Continuous Flow Authorization Page,5-15

Service Login Page,5-16

About Page,5-17

Rotor Catalog Page,5-18

Labware Catalog Page,5-19

Export User Manual Page,5-20

Set System Name Page,5-21

Set Date and Time Page,5-22

Set Sound Page,5-23

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Contents

Select Language Page,5-24

Diagnostic History Page,5-25

Compatible Tubes for Rotor Page,5-26

Zonal Operation Page,5-27

Continuous Flow Operation Page,5-28

Running Home Page,5-29

CHAPTER 6: Maintenance and Troubleshooting,6-1

Field Service,6-1

Rotors and Labware,6-1

Cleaning,6-1

Instrument Surfaces,6-2
Rotor Chamber,6-2
Chamber Door O-ring,6-2

Decontamination,6-2

Sterilization and Disinfection,6-3

Diagnostics/User Messages,6-3

Retrieving Your Sample in Case of Power Failure,6-3

During a Run,6-3

Rotor Spinning at Restoration,6-3

Rotor Stopped at Restoration,6-4
Getting Access to the Sample,6-4
Restoring the Instrument to Operating Condition,6-8

Storage and Transportation,6-8

Supply List,6-9

Replacement Parts,6-9
Supplies,6-9

APPENDIX A: Preinstallation Requirements,A-1

Overview,A-1

Space Requirements,A-1

Safety,A-1
Ventilation,A-2
Temperature,A-2

Electrical Requirements,A-3

APPENDIX B: Special Warranty for the Optima™ XE,B-1

Special Warranty,B-1

vi

APPENDIX C: Diagnostics,C-1

Overview,C-1

Diagnostics/User Messages Chart,C-1

Contents

vii

Contents

viii

Safety and Notices

This chapter presents the important notices that apply to the instrument and describes the
conventions used in the document.

Alerts for Danger, Warning, Caution, Important, and Note

DANGER

CHAPTER 1

DANGER indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury. This signal word is to be limited to the most
extreme situations.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury. May be used to indicate the possibility of
erroneous data that could result in an incorrect diagnosis (does not apply to all
products).

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices. May be used to indicate the possibility of erroneous data that could
result in an incorrect diagnosis (does not apply to all products).

IMPORTANT

Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.

NOTE NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

IMPORTANT is used for comments that add value to the step or procedure being performed.

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1-1

Safety and Notices

Safety Notices

Safety Notices

Before installing, using, or maintaining the instrument, be certain that you know all the following
precautions.

Information

CAUTION

Read all product manuals and consult with Beckman Coulter-trained personnel
before attempting to operate instrument. Do not attempt to perform any
procedure before carefully reading all instructions. Always follow product labeling
and manufacturer’s recommendations. If in doubt as to how to proceed in any
situation, contact your Beckman Coulter Representative.

Installation

CAUTION

This instrument is designed to be installed by a Beckman Coulter Field Service
representative. Installation by anyone other than authorized Beckman Coulter
personnel invalidates any warranty covering the instrument. Also, should the
instrument need to be moved, a Beckman Coulter Field Service representative
must reinstall and re-level the instrument in its new location.

WARNING

Do not place the ultracentrifuge near areas containing flammable or combustible
fluids, or any other source of vapors that could enter the ultracentrifuge air
system and be ignited by the motor.

WARNING

Ultracentrifuge operations generate high energy levels that require precautions
against sudden movements that could result from the rare event of a rotor failure.
Maintain a 30-cm. (1-ft.) clearance envelope around and above the
ultracentrifuge. Do not install any equipment or furniture in this envelope. While
the ultracentrifuge is running, keep the envelope clear of any persons or objects
and do not reach into the envelope except when required to change operating
controls.

1-2

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Replacement Parts

WARNING

Do not replace any centrifuge components with parts not specified for use on this
instrument.

Service

WARNING

Any servicing of this equipment that requires removal of any covers can expose
parts which involve the risk of electric shock or personal injury. Make sure that the
power switch is turned off, and the instrument is disconnected from the main
power source, by removing its power plug from the receptacle. Refer such
servicing to qualified personnel.

Safety and Notices

Safety Notices

1

NOTE

It is your responsibility to decontaminate the instrument and accessories before requesting service by

Beckman Coulter Field Service.

Power Supply

DANGER

To reduce the risk of electrical shock, this instrument uses a three-wire electrical
cord and plug to connect this equipment to earth-ground. Make sure that the
matching wall outlet receptacle is properly wired and earth-grounded.

Fuse Replacement

WARNING

Fuses protect certain electrical circuits within this instrument against overcurrent
conditions. The fuse is not customer replaceable. For continued protections,
please contact Beckman Coulter Field Service.

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1-3

Safety and Notices

Safety Notices

Mechanical Safety

DANGER

For safe operation of the equipment, observe the following:

• Use only the Beckman Coulter rotors and accessories designed for use in this
instrument.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop a rotor by hand.

• Do not move the centrifuge while the drive motor is spinning.

• In the event of a power failure, do not attempt to retrieve the sample from the

instrument for at least one hour. Then follow the instructions for recovery of
the sample in the Maintenance and Troubleshooting chapter.

Chemical and Biological Safety

DANGER

Normal operation may involve the use of solutions and test samples that are
pathogenic, toxic, or radioactive. Such materials require that you take all
necessary safety precautions.

• Handle body fluids with care because they can transmit disease. No known
test offers complete assurance that they are free of micro-organisms.

• Handle all infectious samples according to good laboratory procedures and
methods to prevent spread of disease.

• Because spills may generate aerosols, observe proper safety precautions for
aerosol containment.

• Some of the most virulent infectious agents—Hepatitis (B and C) and HIV (I-V)
viruses, atypical mycobacteria, and certain systemic fungi—require
additional emphasis on aerosol protection.

• Do not run toxic, pathogenic, or radioactive materials in a rotor without taking
appropriate safety precautions.

• Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) require biosafe containment. Materials of a

higher group require more than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health
and safety guidelines.

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Precautions with Liquids

WARNING

Do not place containers holding liquid on or near the chamber door. Liquid, if
spilled, may get into the instrument and damage electrical or mechanical
components.

Volatile Liquids

DANGER

This instrument is not designed for use with materials capable of developing
flammable or explosive vapors. Do not centrifuge such materials (for example,
chloroform or ethyl alcohol) in this instrument nor handle or store them near the
centrifuge.

Safety and Notices

Certification

1

Certification

To ensure full system quality, Beckman Coulter Optima XEs have been manufactured in a registered
ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when used with
Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory
agencies. Declarations of conformity and certificates of compliance are available at
www.beckmancoulter.com.

Scope of Manual

This manual is designed to familiarize you with the Optima XE, its functions, specifications,
operation, and routine operator care and maintenance. Read this entire manual, especially the
safety notices and all safety-related information, before operating the instrument or performing
maintenance.

NOTE

If the instrument is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual and/or the appropriate rotor manual is the sole responsibility of the user.

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1-5

Safety and Notices

CFC-Free Centrifugation

CFC-Free Centrifugation

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the
Optima XE ultracentrifuge.

Radio Interference

This instrument is certified with regard to radio frequency interference for both the United States
and Canada.

United States FCC Certification

This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with this instruction manual, may cause interference to radio communications.
Operation of this equipment in a residential area is likely to cause interference, in which case the
user will be required to correct the interference at his or her own expense.

Canadian Department of Communications Certification

This equipment does not exceed the Class A limits for radio noise emissions from digital apparatus
as set out in the radio interference regulations of the Canadian Department of Communications.

Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites
applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage
radioelectrique édicté par le Ministère des Communications du Canada.

Industry Canada

This Class A digital apparatus complies with Canadian ICES-003.

1-6

Cet appareil numérique de la classe A est conforme à la norme NMB-003 du Canada.

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Software Copyright

The software and other information incorporated into the Optima XE is protected by international
copyright laws. Unauthorized copying, use, distribution, transfer, or sale is a violation of those laws
that may result in civil or criminal penalties. This computer program is also subject to additional
restrictions contained in the following Microsoft OEM Customer License Agreement for Embedded
Systems:

“If you use the Device to access or utilize the services or functionality of Microsoft Windows XP
Server (all editions), or use the Device to permit workstation or computing devices to access or
utilize the services or functionality of Microsoft Windows XP Server, you may be required to obtain
a Client Access License for the Device and/or each such workstation or computing device. Refer to
the End-User License Agreement for Microsoft Windows XP Server for additional information.”

The End-User License Agreement is available on the Microsoft Embedded Systems website.

Safety and Notices

Software Copyright

1

Summary of Instrument Labels

This section provides information for some labels and symbols appearing on the Optima XE
instrument housing. These labels and symbols may be associated with user-serviceable procedures.
Individual hazards associated with a specific procedure in this manual may use these labels and
symbols, and are included in

Warnings or Cautions within the procedures for that task.

Caution Symbol

!

This symbol indicates a caution message and appears adjacent to an explanation or other symbols
that define the caution.

RoHS Caution

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This label and materials declaration table (the Table of Hazardous Substance's Name and
Concentration) are to meet People's Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.

1-7

Safety and Notices

Summary of Instrument Labels

This logo indicates that this electronic information product contains certain toxic or hazardous
elements, and can be used safely during its environmental protection use period. The number in the
middle of the logo indicates the environmental protection use period for the product. The outer
circle indicates that the product can be recycled. The logo also signifies that the product should be
recycled immediately after its environmental protection use period has expired. The date on the
label indicates the date of manufacture.

Universal Serial Bus (USB)

This symbol indicates the location of a universal serial bus (USB) connector.

Ethernet

This symbol indicates the location of an ethernet connector.

Recycling Label

A28219-AA

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:

• the device was put on the European Market after August 13, 2005 and

• the device is not to be disposed of via the municipal waste collection system of any member

state of the European Union.

It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label, please contact your dealer or local Beckman Coulter office for details on the take-back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.

1-8

PN B07711AA

Multiple Compliance

This symbol indicates compliance with:

• certain US Federal Communications Commission Part 15 Rules as described on the label

• N395 – The C-Tick mark is intended for use on products that comply with Australian

Communication Authority (ACA) EMC Requirements.

• 169502 – This label indicates recognition by a Nationally Recognized Testing Laboratory (NRTL)
that the instrument has met the relevant product safety standards.

• CE – This label indicates conformance to various Directives set forth under European Union law.

• Recycling – Refer to the Recycling Label section in this document.

Safety and Notices

Summary of Instrument Labels

1

CAUTION Static Sensitive Area

Indicates an area of the instrument which is sensitive to static electrostatic discharge (ESD). To
prevent damage due to electrostatic discharge, always wear a properly earth-grounded wrist strap
while operating this instrument. For details on proper grounding, see IEEE standard P1100.

DANGER High Voltage

Operation, replacement or servicing of any components where contact with bare, live hazardous
parts could occur, possibly resulting in electric shock, should only be performed by your
Beckman Coulter representative.

PN B07711AA

1-9

Safety and Notices

Summary of Instrument Labels

Protective Ground

This symbol is used to indicate a protective ground. This instrument must be properly grounded.
Do not under any circumstances operate the instrument unless it is properly grounded.

Rotor Rotation

This indicates the direction of instrument rotor rotation.

1-10

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Symbols

Safety and Notices

Symbols

1

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1-11

Safety and Notices

Symbols

1-12

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The Optima XE generates centrifugal forces for the separation of particles. Classified S, it can be
used with all currently manufactured Beckman Coulter preparative rotors.

This chapter describes the major components of the instrument.

For non-In Vitro Diagnostic Use

This Optima XE centrifuge is intended for use as a general purpose laboratory instrument for the
separation of components through the use of relative centrifugal force. Applications may include
the separation of blood, urine, and other bodily fluids, either alone or after the addition of reagents
or other additives; chemicals; industrial or environmental samples.

CHAPTER 2

Description

This centrifuge should be operated by qualified professionals only.

Touch Screen

The touch screen is both the information display and the control input for the instrument. As they
are needed, control buttons appear on the screen. When you select a button, you activate that
control.

The touch screen position is adjustable (both swivel and tilt) to be made viewable from nearly
anywhere in the lab. This is especially useful in environments where a single operator may oversee
a number of instruments.

Each component of the touch screen interface is explained in the following chapters.

Name Rating Plate

A name rating plate is affixed to the rear of the instrument. Always mention the serial number and
model number (available on the About Page ) when contacting Beckman Coulter regarding your
instrument.

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2-1

Description

Rotor Chamber

Rotor Chamber

The rotor chamber is made of aluminum and coated with a chemical-resistant epoxy finish. The
central feature is the rotor drive spindle, but several sensors and control systems are also contained
in the rotor chamber.

Vacuum System

The Optima XE uses a diffusion pump in series with a mechanical vacuum pump to reduce chamber
pressure to a very low level. The system starts automatically when you start a run, or manually
when the chamber door is closed and you use the
Home Page. When the vacuum system is on, the chamber pressure is displayed in microns in the

Vacuum Display/Button.

Vacuum Display/Button on the Footer Bar of the

At the end of a run, press the
attempting to open the door. When the chamber vacuum is reduced to the point that you can open
the door, the system sounds an audible tone. After you open the door, there is a five minute period
where the chamber temperature remains as set (to allow back-to-back runs). Then the chamber
returns to approximate room temperature to minimize condensation collecting in the chamber. To
help keep the chamber dry and clean, keep the door closed whenever possible. If you wish to vent
the chamber before the rotor has come to a complete stop, you can do so as soon as it slows below
3000 rpm.

In zonal or continuous flow operation, the door may remain open while the rotor is spinning up to
the loading speed. The vacuum system is activated when the door is closed and the rotor speed is
above 3000 rpm (after loading the rotor). Normally, you vent the chamber at the end of the run
when the rotor has decelerated to the unloading speed, but you can vent the chamber when the
rotor has decelerated below 3000 rpm if the unloading speed is slower than that.

Vacuum Display/Button to vent the chamber vacuum before

Temperature Sensing and Control

A solid state thermopile in the bottom of the rotor chamber monitors rotor temperature.

The Optima XE uses a solid state thermoelectric refrigeration and heating system without coolant
or water. Cooling is provided by forced air from the fans.

Drive

2-2

When the power is on, the temperature control system starts when the door is closed and the
vacuum system is running.

The frequency-controlled, air-cooled, direct-drive induction motor requires no gears or brushes. In
addition, the drive does not require an oil vacuum seal, external oil reservoir, or continuously
operating damper. Externally cooled by forced air and internally cooled by oil, the drive delivers
ultra-smooth, quiet performance, with high rotor-imbalance tolerance.

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Safety Features

The Optima XE ultracentrifuge has been designed and tested to operate safely indoors at altitudes
up to 2000 m (6562 ft.).

Door

The high-strength structural steel chamber door has a solenoid interlock to prevent operator
contact with a spinning rotor. When the door is closed and a run begins, it locks automatically. It
can be opened only when the power is on and the rotor is at rest with the chamber at atmospheric
pressure. If there is a power failure, you can manually unlock the door as described in the

Maintenance and Troubleshooting chapter.

Barrier Ring

A 41-mm (1.63-in.) steel alloy armor ring acts as the primary barrier, surrounded by a 13-mm
(0.5-in.) vacuum chamber to provide full protection for the operator.

Description

Safety Features

2

Imbalance Detector

An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor
loads are severely out of balance. Even at low speeds, an incorrectly loaded rotor can cause an
imbalance.

Overspeed System

The overspeed system is a safety feature designed to ensure that the rotor does not exceed its
maximum allowable speed. This system includes a photoelectric device in the rotor chamber next
to the drive spindle and an overspeed disk on the bottom of the rotor. Individual rotor manuals
provide information on the correct overspeed disks to be used with each rotor.

The overspeed disk has alternating light and dark sectors. As the rotor spins, the passage of
reflecting and non-reflecting sectors over the photoelectric device generates a series of pulses
detected by the electronic circuitry and software.

After the rotor reaches 600 rpm, the set speed is checked against the overspeed disk. If the set speed
is greater than the maximum speed permitted by the disk, the speed setting is automatically
lowered to the maximum speed of the disk, but the run continues without interruption. An error
message appears to alert you to the change.

Dynamic Rotor Inertia Check (DRIC)

As the rotor accelerates between 15000 and 20000 rpm, the instrument checks the rotor inertia and
calculates the rotor energy for the speed set by the user. If the calculated rotor energy is excessive,
the instrument stops the run with braking to avoid possible damage and sends a diagnostic message
to alert you to this condition.

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2-3

Description

Specifications

Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.

Control Features

Specifications Description

Speed • Set speed:

— 1,000 to rated speed in 100-rpm increments

• Speed display:
— Actual measured rotor speed in 10-rpm increments below 1000 rpm

and 100-rpm increments ≥1000 rpm

• Speed control:
— Actual measured rotor speed, ± 2 rpm at steady state (1,000 rpm

to rated speed)

• Acceleration:
— 10 profiles

• Deceleration:
— 11 profiles, including coast

• Braking:
— Regenerative, power reclamation

Time • Set time:

— Up to 999 hours 59 minutes, including Hold runs

— Up to 3.94780 x 10^14 radians squared per second in

• Time display:
— Indicates time remaining in timed runs, time elapsed in Hold runs,

or estimated time remaining in

• Time accuracy:

— ± 70 ppm (6 seconds/day)

• Run modes:

— Time,

ω²t mode

ω²t runs

ω²t, and RCF

2-4

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Specifications Description

Temperature • Set temperature:

— 0 to 40°C in 1°C increments

• Rotor Temperature (after equilibration):

— ± 0.5°C over entire set temperature range for all rotors except

SW-32

— ± 0.8°C over entire set temperature range for SW-32

• Temperature display:

— Actual rotor temperature in 0.1°C increments

• Ambient temperature range:

— 10 to 35°C

• Humidity restrictions:

— <80% at <35°C (non-condensing)

• Condensation removal:

— Automatic (begins five minutes after vacuum vent)

• Vacuum:

— Below 5 microns (0.7 Pa)

Description

Specifications

2

Ease of Use • Languages:

Physical Data

Specification Description

Dimensions • Width :

— Multi-language support

• Help:

— On-screen, context sensitive

• Diagnostic messages:

— 10,000 messages saved

— 94.0 cm (37 in.)

• Depth:

— 68.1 cm (26.8 in.)

• Height:

— 125.7 cm (49.5 in.)

• Weight

— 485 kg (1068 lb)

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Ventilation Clearances • Sides

— 5.1 cm (2.0 in.)

• Rear

— 15.2 cm (6.0 in.)

2-5

Description

Specifications

Specification Description

Finishes • Rotor pad:

— Coated polycarbonate

• Top and Front surfaces:
— Polyurethane

• Other surfaces:
— Acrylic baking enamel

Electrical • Power requirement:

— 200–240 VAC, 30A branch circuit, 50/60 Hz
— Automatic tap selection on every run

• Electrical supply:
— Class I

• Power Consumption:
— 60W in idle

— 1.0 kW average running in steady state at 90K rpm

• Installation (overvoltage) category:

— II

• LCD Monitor:

— SAW technology touch screen
— Wide tilt range, horizontal and vertical

Environmental • RoHS compliant material:

a. Normally, only non-conductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation

must be expected.

Audible Sounds

The Optima XE instrument will make an audible sound while displaying a corresponding visual
prompt with the following events:

— 100% of content

• Cooling system:

— Thermoelectric (no CFC gases used)

• Noise output (1m in front of instrument):

— <51 dBA (Sound pressure level)
— 7.0 sones (Zwicker Loudness)

• Maximum heat dissipation into room under steady-state conditions:
— 3400 Btu/hr (1.0 kW)

• Pollution degree:

a

— 2

• HEPA filter:
— Available

2-6

• Boot up

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• Start of Run

• End of Run

• Diagnostics/Alert

• Vacuum low enough to open door

• Door open during Zonal or Continuous Flow mode

Available Rotors

All currently manufactured Beckman Coulter floor model ultracentrifuge rotors can be used in the
Optima XE ultracentrifuge. The rotors are described in individual manuals that accompany each
rotor. Information on rotors and accessories is available in the Rotor Catalog accessible from the
touch screen. Additional information is available in Rotors and Tubes for Preparative Ultracentrifuges
(LR-IM) and in the Beckman Coulter Ultracentrifuge Rotors, Tubes & Accessories catalog (publication
BR-8101). The Beckman Coulter website, http://www.beckmancoulter.com, contains additional
information about centrifugation processes and products.

Description

Available Rotors

2

Refer to specific rotor manuals for proper installation and removal of rotors from the instrument.

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2-7

Description

Available Rotors

2-8

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CHAPTER 3

The Touch Screen Interface

This chapter describes the touch screen interface for the Optima XE. Except for the power switch,
all the controls for the instrument are programmed controls that appear on the touch screen.

Figure 3.1 Home Page

Areas on the Screen

The items on the screen may change, depending on the state of the instrument. There are three
areas where items appear on the screen:

• The Header Bar

• The Footer Bar

• The Page Display Area

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3-1

The Touch Screen Interface

Areas on the Screen

Header Bar

Figure 3.2 Header Bar

The area across the top of the screen is called the Header Bar, containing four important items:

Home Page Button

•

•

Menu Button

•

Status Display
Help Button

•

The following sections explain these items.

Home Page Button

Figure 3.3 Home Page Button

The Home Page Button takes you to the Home Page from any other page. When you use Zonal Mode
or Continuous Flow Mode, the

Zonal/Continuous Flow Operation Page replaces the Home Page.

Menu Button

Figure 3.4 Menu Button

The Menu Button presents a list of menu items, allowing you to choose a particular function:

• Options

• References

• About

• Zonal Operation

• Continuous Flow Operation

3-2

• Service Mode

The menu options are explained in the following chapter.

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The Touch Screen Interface

Areas on the Screen

Status Display

Figure 3.5 Ready Status

The Status Display is in the middle of the Header Bar and shows you the current instrument status.
The background color of the header bar changes with the type of status:

• Blue background: system ready (no run in progress).

Figure 3.6 Header Bar - Ready Status

• Green background: in operation (run in progress).

3

Figure 3.7 Header Bar - Running Status

• Green background: in operation (stop in progress).

Figure 3.8 Header Bar - Stopping Status

• Yellow background: warning message.

Figure 3.9 Header Bar - Warning Message

• Red background: fatal error message.

Figure 3.10 Header Bar - Fatal Error Message

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3-3

The Touch Screen Interface

Areas on the Screen

Help Button

Figure 3.11 Help Button

The Help button gives you access to the built-in online help messages. Help messages are described
at the end of this chapter.

Footer Bar

The bottom of the touch screen always shows the footer bar. The footer bar appears in two different
ways, but it always has the

Start Button

Start Button at the left and the Stop Button at the right.

Figure 3.12 Start Button

The Start Button begins a run with the current settings. Use it only after you have set the
parameters for the run.

Stop Button

Figure 3.13 Stop Button

The Stop button stops the current run immediately. This is ordinarily only used for emergencies or
if you mistakenly set too long a run time.

3-4

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Footer Bar on the Home Page

On the Home Page, the middle of the Footer Bar shows three Items:

The Touch Screen Interface

Areas on the Screen

3

• The

Vacuum Display/Button

• The System Name

• The

Figure 3.14 Footer Bar - Home Page

Accel Display/Decel Display/Button

Each of these is explained below.

Footer Bar on Other Pages

When not on the Home Page, the three buttons for Speed, Time and Temperature appear in the
Footer Bar for easy access. To set Acceleration or Deceleration profiles, or to use the
button, simply press
detail within the

• The

• The

• The

Set Speed Display/Button
Set Time Display/Button
Set Temp Display/Button

Home button to return to the Home Page. The following items are described in

Home Page section of the next chapter:

Vacuum

Figure 3.15 Footer Bar on Other Pages

Vacuum Display/Button

As a display, the Vacuum Display/Button shows the current chamber vacuum and the action that
will be performed when the button is pressed. As a control, it serves two functions:

• Before a run, after mounting the rotor and closing the chamber door, press this control to
evacuate the chamber and precondition the chamber to the set temperature.

• After a run, press this control to release the vacuum before opening the chamber door.

System Name

The system name is simply a display. You can set the system name as one of the options on the

System Options Page.

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3-5

The Touch Screen Interface

Help Messages

Accel and Decel Display/Buttons

These are two displays that, together, act as a single button.

As a display, each shows the selected acceleration or deceleration profile currently selected. If you
select either one of them, it takes you to the Set Acceleration/Deceleration Profiles Page, described
in the next chapter.

Page Display Area

The Page Display Area is the main display area between the Header Bar and the Footer Bar. The
pages and help messages all appear in this area. The pages are all described in CHAPTER 5.

Help Messages

When you first select the Help Button, a number of new buttons appear on the screen, giving you
three sets of options:

Item Help

• Item Help

• Global Help

• Exit from Help.

Item Help Buttons appear on-screen items where you can get an item help message. These are brief

pop-up messages describing what the item does. Only one help message remains on the screen at a
time.

Figure 3.16 Item Help Button

3-6

PN B07711AA

Global Help

The Global Help Button appears next to the Help Button. If you select the Global Help Button, the
Page Display Area shows you a description of the current page and all the elements it contains.

Figure 3.17 Global Help Button attached to Help Button

While using Global Help, you also have some additional options:

The Touch Screen Interface

Help Messages

3

• The

Global Help page appears over a ghost image of the screen you were on when you selected

global help.

Figure 3.18 Home Page Help with ghost image

If the ghost image makes it difficult for you to read the Help Page, select the Transparency
Button in the upper right corner.

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Figure 3.19 Tr a n s p ar e n c y B ut to n

The resulting opaque help page eliminates the image of the screen behind it.

3-7

The Touch Screen Interface

Help Messages

Figure 3.20 Home Page Help with opaque screen

Select the Transparency Button again to return to the ghost screen image.

• If you need information about some other aspect of the instrument, you can select the Table of
Contents link at the bottom of the page to see the outline of all the available help pages. You
have access to all of the help pages from the Table of Contents.

Figure 3.21 Help Navigation Buttons

• To navigate through your selected help pages, use the Forward and Back buttons in the upper
left corner.

3-8

PN B07711AA

The Touch Screen Interface

Help Messages

• If the global help page is too large to fit in the page display area, a Scroll Bar appears on the

right side, allowing you to scroll up and down through the page.

Figure 3.22 Zonal/Continuous Flow Operation Help with scroll bar

3

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3-9

The Touch Screen Interface

Help Messages

3-10

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CHAPTER 4

Operations

The touch screen interface for the Optima XE makes operations about as simple as possible.
Although there are many additional things you can do, this chapter outlines basic operations:

• Manual Operation

• Operations with Preconditioning

• Continuous Flow Operation

• Zonal Operation

Manual Operation

Manual operation is a simple procedure you can do from the Home Page.

Before you begin, there are four things you must know:

• What kind of rotor you are using for the run.

• What speed you want for the run.

• How long the run should be.

• What temperature you want for the run.

When you have this information, the procedure for the run is as follows:

1

Start on the Home Page.

2

Set the speed and rotor.

3

Set the time.

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4

Set the temperature.

4-1

Operations

Manual Operation

5

Start the run.

Step 1: Start on the Home Page

Start at the Home Page. If your screen shows any other page, press the Home Page Button in the
upper left corner of the screen.

Figure 4.1 Home Page

4-2

NOTE If your system is in Zonal Mode or Continuous Flow Mode, you have to press the Cancel Button to go

to the Home Page.

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Figure 4.2 Zonal Operation Page with Cancel Button

Operations

Manual Operation

4

Step 2: Set the Speed and Rotor

Press the Set Speed Display/Button on the Home Page to go to the Set Speed Page.

Figure 4.3 Set Speed Page

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4-3

Operations

Manual Operation

When the Set Speed Page appears, follow these steps:

1

Press the Select Rotor Button to go to the Select Rotor and Labware Page.

Figure 4.4 Select Rotor and Labware Page

2

Select the rotor and labware for the run from the list.

3

Select the OK Button to return to the Set Speed Page.

4

If you plan to set the speed in units of relative centrifugal field (RCF), select the RPM/RCF Button.
Note that the RPM/RCF button is only enabled when you have selected a rotor.

5

Use the keypad to set the desired speed. Note that you actually enter hundreds, with two ending
zeroes added to your number. You can also use the

corrections.

6

Select the OK key to accept your entry and dismiss the page.

Backspace and Clear keys to make

4-4

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Step 3: Set the Time

Press the Set Time Display/Button to go to the Set Time Page.

Figure 4.5 Set Time Page

Operations

Manual Operation

4

On the Set Time Page, follow two steps:

1

Use the keypad to set the desired time in hours and minutes. Use the Backspace and Clear keys
to make corrections. You can also use the

countdown to an automatic end. When you use a
select the

2

Select the OK key to accept your entry and dismiss the page.

Stop key (or until the maximum time of 999 hours and 59 minutes has been reached).

Hold key to set the time to a hold state with no

Hold value, the run does not end until you

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4-5

Operations

Manual Operation

Step 4: Set the Temperature

Press the Set Temp Display/Button to go to the Set Temperature Page.

Figure 4.6 Set Temperature Page

On the Set Temperature Page, follow two steps:

1

Use the keypad to set the desired temperature in degrees Celsius. Use the Backspace and Clear
keys to make corrections.

2

Select the OK key to accept your entry and dismiss the page.

4-6

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Step 5: Start the Run

Once the run values are set, start the run:

1

Prepare your samples and place them in the rotor following all proper procedures, including
balanced weight distribution.

2

Preheat or precool the rotor and samples, if necessary.

3

Mount the rotor in the instrument following all the procedures in the rotor manual and
observing all safety procedures and cautions.

4

Close and lock the chamber door. For preconditioning, press the Vacuum Display/Button and
wait until the chamber reaches the set temperature.

Operations

Zonal and Continuous Flow Operation

4

5

Press the Start button.

Figure 4.7 Start Button

When the run begins, wait for the countdown timer to reach zero and the rotor to come to a stop.
You can then remove your rotor and samples.

Zonal and Continuous Flow Operation

WARNING

In zonal and continuous flow operation, the operator is unavoidably exposed to
rotating machinery. For safety, the operator must be properly instructed and
qualified. Guard against accidentally dropping objects, such as pens, pencils, or
hemostats into the chamber. Loose lab coats, neckties, scarves, and long
necklaces should not be worn while operating in the zonal or continuous flow
mode.

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Use only zonal rotors in the zonal mode.

Use only continuous flow rotors in the continuous flow mode.

Zonal and continuous flow operations require special rotors and have some additional hazards in
that samples are loaded and unloaded while the rotor is spinning. To prevent unauthorized users
from attempting these operations, the instrument requires a password (which is 1793). When zonal

4-7

Operations

Zonal and Continuous Flow Operation

or continuous flow operations have been authorized, the Zonal Operation Page or the Continuous

Flow Operation

Page replaces the Home Page. The mode does not end until the run is complete or

the user selects the

Zonal and Continuous Flow operations are very similar. The specific details vary according to the
rotor you are using, but the summary below shows an outline of the procedure.

Figure 4.8 Zonal Page

Cancel Button to end the mode.

There are seven phases to a Zonal or Continuous Flow run:

• Preparing

• Starting

• Loading

• Running

• Unloading

• Stopping

• Finishing

Each phase is outlined in a section below.

Preparing for the Run

The rotor manual details the preparations for a run, which may include cleaning, assembling, and
lubricating the rotor, setting up pumps and tubing, and usually includes overnight cooling to
precondition the rotor, sample and solutions used in the run. When you are ready to install the
rotor, set the parameters for the run:

• Run Speed

• Run Time

4-8

• Temperature

• Load Speed

• Unload Speed

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Operations

Zonal and Continuous Flow Operation

For Continuous Flow operation, you may have two different load speed settings. The initial setting
is for alignment and adjustment checks, which you set in this phase.

4

When everything else is ready, mount the rotor and select the
preconditioning the chamber. When the chamber reaches the set temperature, you are ready to
start the run.

Starting the Run

To start the run, press the Start Button and wait for the rotor to reach loading speed. The status
display at the top of the screen highlights Starting.

For a Continuous Flow run, you need to perform alignment and adjustment checks as detailed in the
rotor manual. You may need to use the
with the
checks, set the new loading speed (if it is different from the adjustment speed) and press the
Button again.

Figure 4.9 Continuous Flow Page

Vacuum Display/Button to begin

Slow to Zero RPM button to make adjustments and restart

Start Button. Refer to Figure 4-9. When the rotor passes the alignment and adjustment

Start

When the rotor reaches the Loading speed, the status display highlights Loading, to show that you
have moved into the next phase.

Loading the Sample

In the Loading phase, you inject the sample into the medium in the rotor. Again, the details depend
on the rotor and the type of operation as detailed in the rotor manual. When you have finished all
the specified loading steps, close the door, and select the
phase.

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Loading Complete button to go to the next

4-9

Operations

Zonal and Continuous Flow Operation

Running the Sample

The instrument highlights Running on the Status Display, accelerates to the set run speed, and
begins counting down the set run time. The run phase ends in one of three ways:

• When the timer countdown reaches zero, the instrument decelerates to the unloading speed
and goes to the Unloading phase.

• If you select the
unloading speed and goes to the Unloading phase.

• If you select the
the unloading phase, and exits Zonal or Continuous Flow mode entirely.

Unloading the Sample

While the rotor is running at unload speed, follow the instructions in the rotor manual to inject the
displacement solution into the rotor and collect the resulting centrifugate sample.

Unload Button to terminate the run early, the instrument decelerates to the

Stop Button to abort the run, the instrument brings the rotor to a halt, skips

Stopping the Run

When you have completed all the defined unloading steps, press the Stop Button to bring the rotor
to a halt.

Finishing the Run

When the rotor comes to a halt, unmount it and perform all the cleanup and follow-up steps given
in the rotor manual. The instrument exits Zonal or Continuous Flow mode when the rotor comes to
a stop.

4-10

PN B07711AA

CHAPTER 5

Functional Pages

This chapter describes the functional pages that you use to control the Optima XE Ultracentrifuge.
They include all of the following:

• Home Page

• Menu Page

• Set Speed Page

• Set Time Page

• Set Speed Time Page (w²t Mode)

• Set Temperature Page

• Set Acceleration/Deceleration Profiles Page

• Select Rotor and Labware

• References Page

• System Options Page

• Zonal Authorization Page

• Continuous Flow Authorization Page

• Service Login Page

• About Page

• Rotor Catalog Page

• Labware Catalog Page

• Export User Manual Page

• Set System Name Page

• Set Date and Time Page

• Set Sound Page

• Select Language Page

• Diagnostic History Page

• Compatible Tubes for Rotor Page

• Zonal Operation Page/Continuous Flow Operation Page

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• Running Home Page

5-1

Functional Pages

Home Page

Home Page

The Home Page is the first operational page to appear after the instrument startup. You return to it
when you select the
most operations.

Home Page Button on the Header Bar. It is the basic page from which you begin

In addition to the Header Bar and the Footer Bar, the

• The

• The

• The

• The

ω²t Display.

Set Speed Display/Button.
Set Time Display/Button.
Set Temp Display/Button.

Home Page contains the following elements:

When you use Zonal Mode or Continuous Flow Mode, the Zonal/Continuous Flow Operation Page
replaces the

Figure 5.1 Home Page

Home Page.

ω²t Display

Figure 5.2 ω²t Display

The
shows the accumuated

5-2

ω²t Display appears only when you have set the ω²t mode on the System Options Page. It

ω²t value for the run in progress. See the Set Speed Time Page for details.

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Set Speed Display/Button

Figure 5.3 Set Speed Display/Button

The Set Speed Display/Button shows the current rotor speed as a large number and also shows the
current run speed setting in small characters across the bottom.

Set Speed Display/Button takes you to the Set Speed Page (or to the Set Speed Time Page (w²t

The

Mode) if the

ω²t mode is active)

Functional Pages

Home Page

5

When you are not on the

Set Time Display/Button

Figure 5.4 Set Time Display/Button

The Set Time Display/Button shows the current remaining run time as a large number and also
shows the current run duration setting in small characters across the bottom. Before you begin a
run, the numbers are the same. During a run, the large number counts down to zero.

When you select the

Page (w²t Mode) if the

When you are not on the

Set Temp Display/Button

Figure 5.5 Set Temp Display/Button

Home page, the Set Speed Display/Button appears in the Footer Bar.

Set Time Display/Button, it takes you to the Set Time Page (Set Speed Time

ω²t mode is active).

Home page, the Set Time Display/Button appears in the Footer Bar.

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The Set Temp Display/Button shows the current rotor temperature as a large number, and also
shows the current temperature setting in small characters across the bottom.

When you select the

When you are not on the Home page, the Set Temp Display/Button appears in the Footer Bar.

Set Temp Display/Button, it takes you to the Set Temperature Page.

5-3

Functional Pages

Menu Page

Menu Page

Figure 5.6 Menu Page

The Menu Page appears when you select the Menu Button in the Header Bar, allowing you access to
the menu choices. Choosing an option dismisses the

Menu page and goes to the selected page.

• The Options Button takes you to the System Options Page.

• The

• The

• The

• The

• The

References Button takes you to the References Page.
About Button takes you to the About Page.
Zonal Operation Button takes you to the Zonal Operation Page.
Continuous Flow Operation Button takes you to the Continuous Flow Authorization Page.
Service Mode Button is used by service personnel only. If you accidently select this button

and go to the

Service Login Page, select the Cancel button to dismiss the page.

Each of these pages is described in a following section.

There is also a

Done button at the bottom of the page that dismisses the page.

5-4

PN B07711AA

Set Speed Page

Figure 5.7 Set Speed Page (No Rotor Selected)

Functional Pages

Set Speed Page

5

Figure 5.8 Set Speed Page (Rotor Selected)

The Set Speed Page appears when you select the Set Speed Display/Button on the Home Page or the
Footer Bar, allowing you to set the speed for the next run. As an alternate, if you have already
selected a rotor, you can set the units in RCF (Relative Centrifugal Field) instead of RPM.

If the instrument is in

In addition to the Header Bar and the Footer Bar, the
elements:

ω²t mode, the Set Speed Time Page appears instead.

Set Speed Page contains the following

PN B07711AA

• The Set Speed Display at the top of the page shows the current Set Speed in RPM or RCF. The
last two digits are always zeroes when the speed is above 1000 RPM.

• The Keypad changes the speed setting. You can use the Clear and Backspace keys to make
corrections.

5-5

Functional Pages

Set Time Page

• The RPM/RCF Button selects the type of speed units. This button is disabled until a rotor is

• The

• The

selected.

Select Rotor Button takes you to the Select Rotor and Labware Page.
Cancel Button discards your changes and dismisses the page.

• The

Set Time Page

Figure 5.9 Set Time Page

OK Button accepts your changes and dismisses the page.

5-6

The Set Time Page appears when you select the Set Time Display/Button on the Home Page or the
Footer Bar, allowing you to set the duration for the next run.

If the instrument is in

In addition to the Header Bar and the Footer Bar, the

• The Set Time Display shows the current

ω²t mode, the Set Speed Time Page appears instead.

Set Time Page contains the following elements:

Set Time setting in hours and minutes.

• The Keypad changes the time setting. You can use the Clear and Backspace keys to make
corrections.

• The

Cancel Button discards your changes and dismisses the page without changing the current

values.

• The

OK Button accepts your changes and dismisses the page.

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Set Speed Time Page (ω²t Mode)

Figure 5.10 Set Speed Time Page (²t Mode)

Functional Pages

Set Speed Time Page (w²t Mode)

5

The Set Speed Time Page appears when the ω²t mode is active and you select either the Set Speed
Button or the
the time and RPM (or RCF) values you enter. Having these all on the same page allows you to set the
values for the next run that produce the desired

Turn the

In addition to the Header Bar and the Footer Bar, the
elements:

• The
keyboard becomes active to set a new speed value. The
speed value.

• The
becomes active to set a new

• The
touch it, the keyboard becomes active to set a new time value. The
the new time value.

• The

• The

• The Keypad changes slightly depending on the value you are entering. For setting time, use the

Hold key to set a hold value. For setting
exponential notation.

Set Time Button on the Home Page or the Footer Bar. The ω²t value is computed from

ω²t value.

ω²t mode on and off with the ω²t Mode Button on the System Options Page.

Set Speed Time Page contains the following

Set Speed Button/Display shows the current Speed in RPM or RCF. When you touch it, the

ω²t value changes to reflect the new

ω²t Button/Display shows the current ω²t value. When you touch it, the keyboard

ω²t value. The time value changes to reflect the new ω²t value.

Set Time Button/Display shows the current time setting in hours and minutes. When you

ω²t value changes to reflect

RPM/RCF Button selects the type of speed units. You must have a rotor selected to use RCF.
Select Rotor Button takes you to the Select Rotor and Labware Page.

ω²t, use the decimal and e keys to enter values in

PN B07711AA

• The

• The

Cancel Button discards your changes and dismisses the page.
OK Button accepts your changes and dismisses the page.

5-7

Functional Pages

Set Temperature Page

Set Temperature Page

Figure 5.11 Set Temperature Page

The Set Temperature Page appears when you select the Set Temperature Button on the Home Page
or the Footer Bar, allowing you to set the temperature for the next run.

In addition to the Header Bar and the Footer Bar, the

Set Temperature Page contains the following

elements:

• The Set Temperature Display shows the current

Set Temperature setting in degrees Celsius.

• The Keypad changes the temperature setting.

• The

• The

To precondition the chamber to the selected temperature, use the
Footer Bar of the

Cancel Button discards your changes and dismisses the page.
OK Button accepts your changes and dismisses the page.

Vacuum Button/Display on the

Home Page.

5-8

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Set Acceleration/Deceleration Profiles Page

Figure 5.12 Set Acceleration/Deceleration Profiles Page

Functional Pages

Set Acceleration/Deceleration Profiles Page

5

The Set Acceleration/Deceleration Profiles Page appears when you select the Accel/Decel Button/
Display in the Footer Bar, allowing you to choose profiles for acceleration and deceleration.

In addition to the Header Bar and the Footer Bar, the
contains the following elements:

• Acceleration lets you to choose an acceleration value by selecting a number.

• Deceleration lets you choose a deceleration value by selecting a number.

• The

• The

The acceleration values are the degree of reduction from the maximum value. The acceleration
value of zero is the maximum (no reduction). The acceleration value of 9 is the slowest (maximum
reduction). The same applies to the deceleration values. The value 10 is an absolute reduction,
eliminating all braking and allowing the rotor to coast to a stop.

Slower (numerically higher) acceleration and deceleration values minimize sample-to-gradient
interface disturbance. Each acceleration profile has a designated time that it takes to reach a
specified speed. After that, it uses maximum acceleration to reach run speed. For a deceleration
profile, the instrument uses maximum deceleration until it reaches the specified speed, then takes
the designated time to slow to a stop.

Cancel Button discards your changes and dismisses the page.
OK Button accepts your changes and dismisses the page.

Set Acceleration/Deceleration Profiles Page

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5-9

Functional Pages

Set Acceleration/Deceleration Profiles Page

Example Acceleration and Deceleration Profiles

The values associated with each acceleration and deceleration profile are given in the tables below.

NOTE

The values shown should be considered as approximations for time and rpm.

Acceleration Deceleration

Profile # Time RPM Profile # Time RPM

0 (Max) 0:00 0 0 (Max) 0:00 0

12:00170 12:00170

22:40350 22:40350

3 3:00 500 3 3:00 500

4 3:00 170 4 3:00 170

5 4:00 350 5 4:00 350

6 4:30 500 6 4:30 500

7 4:00 170 7 4:00 170

85:20350 85:20350

96:00500 96:00500

10 Coast

For example, at an acceleration profile value of 5, the instrument takes four minutes to accelerate
to 350 rpm, then proceeds to the run speed at maximum acceleration. For a deceleration profile of
3, the instrument uses maximum deceleration until it reaches 500 rpm, then takes three minutes to
slow to a stop.

The exception is a deceleration profile of 10, for which no braking is applied at all. This is generally
not used for high-speed runs because of the extremely long length of time it would take for the

rotor to come to a stop.

5-10

PN B07711AA

Select Rotor and Labware

Figure 5.13 Select Rotor and Labware Page

Functional Pages

Select Rotor and Labware

5

The Select Rotor and Labware Page appears when you select the Button on either the Set Speed Page

Set Speed Time Page, allowing you to select a rotor for the next run.

or the

This is different from the Rotor Catalog Page, which shows rotor specifications, but does not select
one for your next run.

In addition to the Header Bar and the Footer Bar, the
following elements:

• The Rotor Catalog List shows the available rotors for the instrument. You can select a rotor
from this list. If the No Rotor value is selected, you cannot use RCF units on the

• The

• The

For details about each rotor or labware, use the
selection.

Cancel Button dismisses the page and any changes.
OK Button accepts the selection and dismisses the page.

Select Rotor and Labware Page contains the

Set Speed Page.

Rotor Catalog Page. This page only lists names for

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5-11

Functional Pages

References Page

References Page

Figure 5.14 References Page

The References Page appears when you select the References Button in the Menu Page, allowing you
to choose from the available reference materials to view.

In addition to the Header Bar and the Footer Bar, the

References Page contains the following

elements:

• The

• The

• The

• The

Rotor Catalog button takes you to the Rotor Catalog Page.
Labware Catalog button takes you to the Labware Catalog Page.
Export User Manual button takes you to the Export User Manual Page.
Done Button dismisses the page.

5-12

PN B07711AA

System Options Page

Figure 5.15 System Options Page

Functional Pages

System Options Page

5

The System Options Page appears when you select the Options Button in the Menu Page, allowing
you to choose any of the available system option setting pages.

In addition to the Header Bar and the Footer Bar, the
elements:

• The

• The

• The

• The

• The

• The

• The

Select Language Button takes you to the Select Language Page.
Set Sound Button takes you to the Set Sound Page.
Diagnostic History Button takes you to the Diagnostic History Page.
Set Date and Time button takes you to the Set Date and Time Page.
Set System Name Button takes you to the Set System Name Page.

ω²t Mode Button toggles the ω²t mode off and on. The mode is on when the button is

highlighted. See the

Done Button dismisses the page.

Set Speed Time Page for details.

System Options Page contains the following

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5-13

Functional Pages

Zonal Authorization Page

Zonal Authorization Page

Figure 5.16 Zonal Authorization Page

The Zonal Authorization Page appears when you select the Zonal Operation Button on the Menu Page,
allowing you to use Zonal mode for the next run.

In addition to the Header Bar and the Footer Bar, the

Zonal Authorization Page contains the

following elements:

• The Authorize Display allows you to enter the authorization password (which is 1793). For
security reasons, the display shows bullets in place of the numbers you enter.

• The Keypad for entering the password.

• The

• The

Cancel Button dismisses the page without entering Zonal mode.
Authorize Button accepts the password you have entered and (if the password is the correct

one) takes you to the Zonal Operation Page, which replaces the

Home Page while you are using

Zonal mode.

5-14

PN B07711AA

Continuous Flow Authorization Page

Figure 5.17 Continuous Flow Authorization Page

Functional Pages

Continuous Flow Authorization Page

5

The Continuous Flow Authorization Page appears when you select the Continuous Flow Operation
Button on the

In addition to the Header Bar and the Footer Bar, the
the following elements:

• The Authorize Display allows you to enter the authorization password (which is 1793). For
security reasons, the display shows bullets in place of the numbers you enter.

• The Keypad for entering the password.

• The

• The

one) takes you to the Continuous Flow Operation Page, which replaces the
are using Continuous Flow mode.

Menu Page, allowing you to use Continuous Flow mode for the next run.

Continuous Flow Authorization Page contains

Cancel Button dismisses the page without entering Continuous Flow mode.
Authorize Button accepts the password you have entered and (if the password is the correct

Home Page while you

PN B07711AA

5-15

Functional Pages

Service Login Page

Service Login Page

Figure 5.18 Service Login Page

The Service Login Page appears when you select the Service Mode Button on the Menu Page.

This page is for service personnel only. If you accidently find yourself on the
select the

Cancel button to dismiss the page.

Service Login Page,

5-16

PN B07711AA

About Page

Figure 5.19 About Page

Functional Pages

About Page

5

The About Page appears when you select the About Button on the Menu Page. This page presents
system information about your instrument.

In addition to the Header Bar and the Footer Bar, the
elements:

• The

• The

NOTE

• The Available Drives list shows all the available USB drives. If the list is empty, the prompt

• The

Done button dismisses the page.
Export button takes you to the export About Page.

Do not remove the USB drive while a data transfer is in progress.

“Insert USB Drive” appears below the list area. Select an available USB drive to receive the
information.

Export Button transfers the information to the selected USB drive.

About Page contains the following operating

PN B07711AA

5-17

Functional Pages

Rotor Catalog Page

Rotor Catalog Page

Figure 5.20 Rotor Catalog Page

The Rotor Catalog Page appears when you select the Rotor Catalog Button on the References Page,
allowing you to examine the detailed specifications for all the rotors available for your instrument.

This is different from the

Speed Page.

Select Rotor and Labware Page where you choose a rotor from the Set

In addition to the Header Bar and the Footer Bar, the Rotor Catalog Page contains the following
elements:

• The List of Rotors on the left side of the screen shows all the available rotors for the instrument.
You can scroll through this list and select a rotor to view the detailed information about it.

• The Rotor Details on the right side of the screen shows all the details for the selected rotor on
the List of Rotors.

• The

• The

Back Button returns you to the References Page.
Labware Button takes you to the Compatible Tubes for Rotor Page to show you the labware

for the selected rotor. Note that this is a small subset of all the labware shown on the

Catalog

Page.

Labware

5-18

PN B07711AA

Labware Catalog Page

Figure 5.21 Labware Catalog Page

Functional Pages

Labware Catalog Page

5

The Labware Catalog Page appears when you select the Labware Catalog Button on the References
Page, allowing you to examine the detailed specifications for the labware available for the rotors
your instrument can use.

This is different from the
for a particular rotor when you are on the

In addition to the Header Bar and the Footer Bar, the Labware Catalog Page contains the following
elements:

• The List of Labware on the left side of the screen shows the labware by volume, type, and part
number. Scroll through this list and select a particular labware to view the detailed information
about it.

• The Labware Details on the right side of the screen shows all the details for the selected labware
on the List of Labware.

• The

Back Button returns you to the References Page.

Compatible Tubes for Rotor Page, which shows only the labware available

Rotor Catalog Page.

PN B07711AA

5-19

Functional Pages

Export User Manual Page

Export User Manual Page

Figure 5.22 Export User Manual Page

Selection of the Export User Manual Button allows you to copy the User Manual to a USB drive.

NOTE Do not remove the USB drive while a data transfer is in progress.

In addition to the Header Bar and the Footer Bar, the Export User Manual Page contains the following
elements:

• The Available Drives list shows all the available USB drives. If the list is empty, the prompt
“Insert USB Drive” appears below the list area. Select an available USB drive to receive the
information.

• The

• The

Back Button returns you to the previous page.
Export Button transfers the information to the selected USB drive.

5-20

PN B07711AA

Set System Name Page

Figure 5.23 Set System Name Page

Functional Pages

Set System Name Page

5

The Set System Name Page appears when you select the Set System Name Button on the System

Options Page, allowing you to set the System Name that appears in the Footer Bar on the

In addition to the Header Bar and the Footer Bar, the
elements:

• The Set System Name Display shows the current System Name.

• The Keyboard for entering the name. It includes special keys for different character modes and

corrections.

• The

• The

The other keys work much like standard keyboard keys, except that the Shift and Special Character
Set keys work as toggle (like a standard Caps Lock key). There are also Clear and Backspace keys
with symbolic markings.

Cancel Button ignores any changes you have made and returns to the System Options Page.
OK Button accepts your changes and returns to the System Options Page.

Set System Name Page contains the following

Home Page.

PN B07711AA

5-21

Functional Pages

Set Date and Time Page

Set Date and Time Page

Figure 5.24 Set Date and Time Page

The Set Date and Time Page appears when you select the Set Date and Time Button on the System

Options Page, allowing you to set the instrument's internal time and date.

In addition to the Header Bar and the Footer Bar, the

Set Date and Time Page contains the following

elements:

• The Month-Day-Year controls let you set the date. Simply select the arrow button above one of
the numbers to make it larger or select the button below to make it smaller.

• The Hour-Minute-AM/PM controls let you set the time. They work the same as the date
controls except that the AM/PM value has only one arrow button to change the setting.

• The

Select Time Format Buttons set the way time appears on the instrument. The 12 Hour

format button selects a 12 hour display with AM and PM. The 24 Hour format button selects a
24 hour display without AM and PM notation. With some language selections, the 12 hour
option may be unavailable.

• The

Cancel Button discards any changes you made on this screen and returns to the System

Options Page.

• The

OK Button accepts your changes and returns to the System Options Page.

5-22

PN B07711AA

Set Sound Page

Figure 5.25 Set Sound Page

Functional Pages

Set Sound Page

5

The Set Sound Page appears when you select the Set Sound Button on the System Options Page,
allowing you to set the sound volume and enable or disable the key click option.

In addition to the Header Bar and the Footer Bar, the
elements:

• The

• The

• The

• The

Volume buttons let you to choose one of the four system volumes: Mute (silent), Low,

Medium, or High.

Key Click buttons let you choose if the key click is On or Off.
Cancel Button discards your changes and returns you to the System Options Page without

changing the current volume and key click settings.

OK button accepts your changes and returns you to the System Options Page.

Set Sound Page contains the following

PN B07711AA

5-23

Functional Pages

Select Language Page

Select Language Page

Figure 5.26 Select Language Page

The Select Language Page appears when you select the Select Language Button on the System

Options Page, allowing you to choose the language the instrument uses in operations.

In addition to the Header Bar and the Footer Bar, the

Select Language Page contains the following

elements:

• The List of Languages shows all the languages and countries available for the instrument. Scroll
through this list and select a language and country.

• The

• The

Cancel Button ignores your selection and returns you to the System Options Page.
OK Button accepts your selection and returns you to the System Options Page with the

newly-selected language active on all pages.

NOTE

Be careful that you do not select a language that you do not understand. You will find it difficult to
return to this page and restore the language setting (unless you can remember Menu, upper left button
for Options, and then upper left button for Select Language).

5-24

PN B07711AA

Diagnostic History Page

Figure 5.27 Diagnostic History Page

Functional Pages

Diagnostic History Page

5

The Diagnostic History Page appears when you select the Diagnostic History Button on the System

Options Page, allowing you to examine and export the details of incidents that caused a diagnostic

message on the instrument (warnings and error messages).

In addition to the Header Bar and the Footer Bar, the
elements:

• The List of Events on the left side of the screen shows all the diagnostic events for the
instrument. Scroll through this list and select an event to view the detailed information about
it.

• The Event Details on the right side of the screen shows all the details for the selected event on
the List of Events.

• The

• The

NOTE

• The Available Drives list shows all the available USB drives. If the list is empty, the prompt

• The

Back Button returns you to the System Options Page.
Export Button takes you to the Export Diagnostic Page to let you copy the diagnostic

history to a USB drive.

Do not remove the USB drive while a data transfer is in progress.

“Insert USB Drive” appears below the list area. Select an available USB drive to receive the
information.

Export Button transfers the information to the selected USB drive.

Diagnostic History Page contains the following

PN B07711AA

5-25

Functional Pages

Compatible Tubes for Rotor Page

Compatible Tubes for Rotor Page

Figure 5.28 Compatible Tubes for Rotor Page

The Compatible Tubes for Rotor Page appears when you select the Labware Button on the Rotor

Catalog

Page, allowing you to examine the detailed specifications for the labware for the selected

rotor.

This is different from the

Labware Catalog Page, which shows the labware for all rotors.

In addition to the Header Bar and the Footer Bar, the Compatible Tubes for Rotor Page contains the
following elements:

• The List of Labware on the left side of the screen shows the labware for the selected rotor by
volume, type, and part number. Scroll through this list and select a particular labware to view
the detailed information about it.

• The Labware Details on the right side of the screen show all the details for the selected labware
on the List of Labware.

• The

Back Button returns you to the Rotor Catalog Page.

5-26

PN B07711AA

Zonal Operation Page

Figure 5.29 Zonal Operation Page

Functional Pages

Zonal Operation Page

5

The Zonal Operation Page appears when you enter the correct password and select the Authorize
Button from the

When you use Zonal mode, the

In addition to the Header Bar and the Footer Bar, the
elements:

• The Status Display shows the current step in the Zonal run procedure. See Zonal and

Continuous Flow Operation in the Operations chapter for details.

• The

• The

• The Load Speed Display shows the sample loading speed. Use the

• The

• The Unload Speed Display shows the sample unloading speed. Use the arrow buttons above and

• The

Loading Complete Button ends the sample loading step and proceeds to the running step.
Unload Button terminates the running step early and proceeds to the sample unloading

step.

below to increase or decrease the sample loading speed.

Vacuum Display/Button shows the current vacuum. You press it to begin evacuating and

preconditioning the chamber at the beginning of a run, or to release the vacuum at the end of
a run.

below to increase or decrease the sample unloading speed.

Cancel Button ends Zonal Mode before loading is complete and returns you to the Home

Page.

Zonal Authorization Page, putting the instrument in Zonal mode.

Zonal Operation Page replaces the Home Page until Zonal mode ends.

Zonal Operation Page contains the following

arrow buttons above and

PN B07711AA

Use the Footer Bar for setting the run speed, time, and temperature.

5-27

Functional Pages

Continuous Flow Operation Page

Continuous Flow Operation Page

Figure 5.30 Continuous Flow Operation Page

The Continuous Flow Operation Page appears when you enter the correct password and select the

Authorize Button from the Continuous Flow Authorization Page, putting the instrument in

Continuous Flow mode.

When you use Continuous Flow mode, the

Continuous Flow Operation Page replaces the Home Page

until Continuous Flow mode ends.

In addition to the Header Bar and the Footer Bar, the

Continuous Flow Operation Page contains the

following elements:

• The Status Display shows the current step in the Continuous Flow Run procedure. See Zonal and

Continuous Flow Operation in the Operations chapter for details.

• The

• The

Loading Complete Button ends the sample loading step and proceeds to the running step.
Unload Button terminates the running step early and proceeds to the sample unloading

step.

• The Load Speed Display shows the sample loading speed. Use the arrow buttons above and
below to increase or decrease the sample loading speed.

• The

Vacuum Display/Button shows the current vacuum. Press it to begin evacuating and

preconditioning the chamber at the beginning of a run, or to release the vacuum at the end of
a run.

• The Unload Speed Display shows the sample unloading speed. Use the arrow buttons above and
below to increase or decrease the sample unloading speed.

• The

Slow to Zero rpm Button is for preliminary steps which require bringing the rotor to a stop

temporarily during the initial loading adjustments.

• The

Cancel Button ends Continuous Flow Mode before loading is complete and returns you to

Home Page.

the

5-28

Use the Footer Bar for setting the run speed, time, and temperature.

PN B07711AA

Running Home Page

Figure 5.31 Running Home Page

Functional Pages

Running Home Page

5

The Running Home Page appears when there is an actual run in progress.

In addition to the Header Bar and the Footer Bar, the Running Home Page contains the following
elements:

• The

• The Set Time Display shows the current

• The Set Temperature Display shows the current

The
progress. The button toggles between evacuating and venting. Press it before starting a run (after
the door is closed) to begin evacuating and pre-conditioning the chamber. Press it at the end of a
run (assuming speed is less than 3000 rpm and decelerating) to release the vacuum (i.e., vent the
chamber).

Press the
of a run.

Press the
of a run.

Set Speed Display shows the current Set Speed progress of the run in RPM or RCF. The last

two digits are always zeroes when the speed is above 1000 RPM.

Set Time progress of the run in hours and minutes.

Set Temperature of the run in degrees Celsius.

Vacuum Display/Button on the Footer Bar shows the current vacuum of the run that is in

Accel (acceleration) button on the Footer Bar to select a new profile for the acceleration

Decel (deceleration) button on the Footer Bar to select a new profile for the deceleration

PN B07711AA

5-29

Functional Pages

Running Home Page

5-30

PN B07711AA

This chapter contains care and maintenance procedures to be performed regularly.

Field Service

For any maintenance not covered in this manual, contact Beckman Coulter Field Service for
assistance. USA customers can call 1-800-742-2345. For international contacts, see the website at
www.beckmancoulter.com or use the contact numbers on the inside front cover of this book.

CHAPTER 6

Maintenance and Troubleshooting

NOTE

It is your responsibility to decontaminate the instrument, as well as any rotors and accessories,
before requesting service by Beckman Coulter Field Service.

Rotors and Labware

You also need to maintain rotors and labware. You can find the approved rotors and labware on the

Reference Page as described in the previous chapter. Refer to the applicable rotor and labware

documents for detailed instructions on their care.

Cleaning

The methods and materials used in the following procedures have been tested by Beckman Coulter
and will not damage the instrument if used as instructed.

CAUTION

Before using any other materials or methods, check with Beckman Coulter to
verify that they will not damage the instrument.

PN B07711AA

6-1

Maintenance and Troubleshooting

Decontamination

Instrument Surfaces

Clean instrument surfaces using a cloth dampened with a mild detergent solution, such as Beckman
Solution 555™.

CAUTION

Be careful not to spill liquid on the instrument where electrical or mechanical
components could get damaged.

Rotor Chamber

The rotor chamber is coated with epoxy resin paint. To clean the chamber, wipe it with a cloth
dampened with a mild detergent, such as Beckman Solution 555.

Chamber Door O-ring

The chamber door O-ring is Buna N rubber. Clean it with a tissue or soft cloth every 3 or 4 months.

If the O-ring becomes worn or damaged, replace it. Lightly coat the new O-ring with silicone
vacuum grease (335148) to ensure an optimum vacuum seal.

NOTE

Instrument O-rings have not been designed as bioseals for aerosol containment.

Decontamination

If the instrument and/or accessories are contaminated with radioactive or pathogenic solutions,
follow appropriate decontamination procedures as determined by your laboratory safety officer.
Refer to Chemical Resistances (publication IN-175), or contact Beckman Coulter Field Service to
ensure that the decontamination method does not damage any part of the instrument (or
accessories).

6-2

PN B07711AA

Sterilization and Disinfection

WARNING

While Beckman Coulter has tested these methods and found that they do not
damage the instrument, no guarantee of sterility or disinfection is expressed or
implied. When sterilization or disinfection is a concern, consult your laboratory
safety officer regarding proper methods.

The top working surface is finished with urethane paint. The sides are finished with general purpose
paint. You can use Ethanol (70%) on both these surfaces.

WARNING

Ethanol is a volatile liquid that cannot be used on or near an operating instrument
due to fire hazard.

Maintenance and Troubleshooting

Sterilization and Disinfection

6

Diagnostics/User Messages

When a condition arises that requires operator attention, the windshield turns yellow or red. A
dialog will automatically open showing the diagnostic message. User messages communicate
information about the ultracentrifuge or alert you to an abnormal condition. For a list of the
possible malfunctions and their corrective actions, see APPENDIX C, Diagnostics.

Retrieving Your Sample in Case of Power Failure

The instrument responds to a power failure during operations in two different ways:

• Power failed during a run and the rotor is still spinning when power is restored.

• Power failed during a run and the rotor has stopped when power is restored.

During a Run

If a power failure occurs during a run, the rotor begins to decelerate with the brake off. The
corrected run time is determined when power is restored and, if the set run time has not elapsed,
the run will resume as described. Note that a rotor decelerating without the brake may take hours
to come to a complete stop.

PN B07711AA

Rotor Spinning at Restoration

If the rotor is still spinning when power is restored, the instrument takes the following steps:

• Return to the set speed.

• Resume incrementing the run timer.

• Set a diagnostic message to alert you that a power failure occurred during the run.

6-3

Maintenance and Troubleshooting

Retrieving Your Sample in Case of Power Failure

Rotor Stopped at Restoration

If the rotor has stopped spinning when power is restored, the instrument cancels the run and sends
a diagnostic message to alert you to that the run was cancelled due to a power failure.

Getting Access to the Sample

If a power failure lasts for several hours, you may have to remove the sample from the rotor while
there is no power to the instrument. The procedure requires removing the front panel, which
should be done only by qualified service personnel.

WARNING

Any maintenance procedure requiring removal of a panel exposes the operator to
the possibility of electrical shock and/or physical injury. If any such procedure
becomes necessary, turn the power switch OFF, and then disconnect the
instrument from the main power source by removing its power plug from the
receptacle. Refer the maintenance to qualified service personnel.

To get access to the rotor, follow these steps:

• Disconnect the power by removing its power plug from the receptacle.

• Remove the front panel.

• Vent the chamber to release the vacuum.

• Release the door lock.

• Open the door.

WARNING

The following procedure should be performed only when absolutely necessary and
only by qualified service personnel.

Check Power

Check that the power switch is in the OFF position and the instrument is disconnected from the
power (i.e.,the power plug is removed from its receptacle).

Remove the Front Panel

To remove the front panel:

1

Locate the three front panel latch screws and use a small flat-head screwdriver to turn each
latch screw counter-clockwise until each one is loose. Refer to Figure 6.1.

6-4

PN B07711AA

Figure 6.1 Panel screws and Tabs

Maintenance and Troubleshooting

Retrieving Your Sample in Case of Power Failure

6

PN B07711AA

1. Panel Tabs

2. Top Panel

2

When all three latch screws are loose, use your hand to lift the top panel until it pops loose.

3

Lift the front panel until it is free and tilt the top edge towards you.

4

Lift the front panel off the tabs that secure it at the bottom and put it in a safe place.

3. Front Pa nel

4. Latch Screws

6-5

Maintenance and Troubleshooting

Retrieving Your Sample in Case of Power Failure

WARNING

When working on the inside of the instrument, be careful not to touch any wires
or circuitry.

Figure 6.2 Internal Parts

6-6

1. Fan Housing

2. Vac uum Ven t Port C ap

3. Door Interlock Assembly

5

Listen carefully for any sounds coming from the drive and touch the fan housing to feel for
vibrations. If you detect any sounds or vibrations, do not go further. The rotor is still spinning

and you must wait for it to come to a stop.

PN B07711AA

Maintenance and Troubleshooting

Retrieving Your Sample in Case of Power Failure

Vent the Chamber

Although you checked for noise and vibration, there is still a possibility that the rotor is still
spinning. If it is still spinning, you can hear a whining noise when you begin to vent the chamber.
If you hear the whining noise when you begin to vent the chamber, you must close the port
immediately and wait for the rotor to come to a stop. The following steps describe the procedure
you must follow:

1

Locate the vacuum vent port cap on the side of the rotor chamber.

2

Turn the cap very slowly counter-clockwise until you can hear the hiss of air entering the
chamber.

3

If you hear a whining noise, immediately turn the cap clockwise to close it and wait until the
rotor has had time to come to a stop (at least an hour) before attempting to vent the chamber

again.

6

4

When air flows into the chamber without a whining noise, remove the cap completely.

Release the Door Lock

Locate the door interlock assembly. Pull down on the interlock pin until it locks in the downward
position.

Open the Door

Open the chamber door carefully. If the rotor is still spinning, even at a slow speed, close the door
and wait.

WARNING

NEVER attempt to slow or stop the rotor by hand.

With the door open and the rotor stopped, you can retrieve your sample.

WARNING

Do not attempt to run the instrument before restoring it to a safe operating
condition as described in the following section.

PN B07711AA

6-7

Maintenance and Troubleshooting

Storage and Transportation

Restoring the Instrument to Operating Condition

After retrieving your sample, restore the instrument to operating condition as follows:

1

Close the chamber door.

2

Replace and tighten the vacuum vent port cap. It should be snug, but do not overtighten it.

3

Place the front panel in the tilted position with the top edge toward you, the sides aligned with
the sides of the instrument, and the tabs at the bottom edge inserted into the lip at the base of

the instrument.

4

Lift the front edge of the top panel a few inches and insert the upper edge of the front panel
under it. Push back gently to engage the tabs, then push down.

5

For each of the three latch screws, use the small flat-head screwdriver to turn it clockwise until
snug.

6

After the panels are all secure, reconnect the instrument to the power supply.

The instrument is again ready for use when power is restored.

Storage and Transportation

To ensure that the instrument does not get damaged, contact Beckman Coulter Field Service for
specific instructions and/or assistance in preparing the equipment for transport or long-term
storage. Temperature and humidity requirements for storage should meet the environmental
requirements described in

Appendix A: Preinstallation Requirements.

6-8

PN B07711AA

Supply List

Contact Beckman Coulter Sales for assistance ordering parts and supplies. Customers in the United
States call 1-800-742-2345. For international contacts, see the website at www.beckmancoulter.com
or use the contact numbers on the inside front cover of this book. A partial list of supplies is given
below for your convenience. See the Beckman Coulter Ultracentrifuge Rotors, Tubes & Accessories
catalog (BR-8101, available at www.beckmancoulter.com) for detailed information on ordering
rotors, tubes, and accessories.

Replacement Parts

Chamber O-ring 801778

Description Part Number

Maintenance and Troubleshooting

Supply List

6

Supplies

Description Part Number

Spinkote lubricant (1 oz) 306812

Silicone vacuum grease (2 oz) 335148

Beckman Solution 555 (1 qt) 339555

Logbook for preparative ultracentrifuges 330049

Master rotor logbook 339587

NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com

PN B07711AA

6-9

Maintenance and Troubleshooting

Supply List

6-10

PN B07711AA

Overview

APPENDIX A

Preinstallation Requirements

NOTE Do not attempt to install or turn on the power to the Optima XE. Its purchase price includes installation

by Beckman Coulter personnel. Installation by anyone other than authorized Beckman Coulter personnel
invalidates the instrument warranty.

Preinstallation requirements have been sent prior to shipment of the instrument. Copies are also
attached to the outside of the shipping container. The following information is provided in case the
instrument must be relocated. Contact Beckman Coulter Field Service to adjust and level the
instrument if it must be moved. The pads on each leveling leg are designed to prevent possible
rotation of the instrument in the case of a rotor mishap.

Space Requirements

Space requirements include specifications for safety, ventilation, and temperature.

Safety

Locate the ultracentrifuge in a clean, safe, uncluttered environment free of volatile vapors that
could be ignited by the operation of the centrifuge.

WARNING

Do not place the ultracentrifuge near areas containing flammable reagents or
combustible fluids. Vapors from these materials could enter the ultracentrifuge
air system and be ignited by the motor.

Although the possibility of a rotor failure is remote, your planning should include a safety space
around the instrument of 12 inches (30.5 cm.) to allow for the movement of the instrument in the
event that a rotor failure occurs at high rotational speed. If you decide to install the instrument in
an area where furniture, equipment, or a wall is within this safety space, you run the risk of damage
to anything within this space in the event of a rotor failure.

PN B07711AA

A-1

Preinstallation Requirements

Space Requirements

WARNING

Maintain a 30-cm (1-ft.) clearance envelope around the ultracentrifuge while it is
running. No persons or any hazardous materials should be within this clearance
boundary while the ultracentrifuge is operating except to change operating
controls, if required.

Figure A.1 Safety and ventilation space

Ventilation

If you choose to install the instrument with less than the 12 in./30.5 cm. safety clearence, you must
at least provide a required ventilation and service access clearence of 6 in. (15.2 cm.) behind the
instrument and 2 in. (5.1 cm.) on each side. In addition, the ultracentrifuge must have adequate air
ventilation to ensure compliance to local requirements for vapors produced during operation.

Temperature

The instrument operates within specifications in a laboratory with ambient temperatures ranging
from 10 to 35°C.

A-2

PN B07711AA

Electrical Requirements

Instrument Rating: 220 to 240 VAC, 50 Hz, 20 A

Power Line Range: 180 to 264 VAC, 60 or 50 Hz (single-phase), 30 A

To reduce risk of electrical shock, this equipment uses a three-wire electrical cord (3.05 m; 10 ft.)
and plug (see Figure A.2) to connect the equipment to earth ground. In regions where the
instrument is supplied with an unterminated cord, a plug that meets local electrical and safety
requirements must be supplied. (Contact your local Beckman Coulter office for specific information
regarding these requirements.) See Table A.1 for the required wire connections. Make sure that the
matching wall outlet is located near the centrifuge and is easily accessible, properly wired and
earth-grounded.

200 to 240 VAC, 50/60 Hz, 20 A

Preinstallation Requirements

Electrical Requirements

A

NOTE

Figure A.2 Electrical Connection

The power plug serves as the Disconnecting Device and must remain easily accessible.

1. 30-ampere Circuit Protector

2. Measured Line Voltage

3. North American Plug

4. Wall Outlet: NEMA 6-30 R

5. Earth Ground

6. 30-ampere Circuit Protector

To ensure safety, the instrument should be wired to a remote emergency switch (preferably outside
the room where the ultracentrifuge is housed, or adjacent to the exit from that room). Refer to
Table A.1.

PN B07711AA

Table A.1 Required Wire Connections

Symbol

Wire Insulation Color Terminal

Green/Yellow Earth ground

Light Blue Neutral N L

Brown Live or Line L L

Harmonized North American

A-3

Preinstallation Requirements

Electrical Requirements

A-4

PN B07711AA

Special Warranty

Subject to the exceptions and upon the conditions specified below, Beckman Coulter, Inc., agrees to
correct, either by repair, or, at its election, by replacement, any defects of material or workmanship
which develop within one (1) year after delivery of the Optima Ultracentrifuge (the product), to the
original Buyer by Beckman Coulter, or by an authorized representative, provided that investigation
and factory inspection by Beckman Coulter discloses that such defect developed under normal and
proper use.

APPENDIX B

Special Warranty for the Optima™ XE

Some components and accessories by their nature are not intended to and will not function for as
long as one (1) year. If any such component or accessory fails to give reasonable service for a
reasonable period of time, Beckman Coulter will repair or, at its election, replace such component
or accessory. What constitutes either reasonable service and a reasonable period of time shall be
determined solely by Beckman Coulter.

Replacement

Any product claimed to be defective must, if requested by Beckman Coulter be returned to the
factory, transportation charges prepaid, and will be returned to Buyer with the transportation
charges collect unless the product is found to be defective, in which case Beckman Coulter will pay
all transportation charges.

Beckman Coulter makes no warranty concerning products or accessories not manufactured by it.
In the event of failure of any such product or accessory, Beckman Coulter will give reasonable
assistance to the Buyer in obtaining from the respective manufacturer whatever adjustment is
reasonable in light of the manufacturer’s own warranty.

Damage to the instrument while operating a rotor not of Beckman Coulter manufacture is not
covered by warranty or service contract terms. Further, Beckman Coulter shall be released from all
obligations under all warranties either expressed or implied, if the product covered hereby is
repaired or modified by persons other than its own authorized service personnel, unless such repair
is made by others who meet qualifications similar to those required of Beckman Coulter’s service
personnel, or unless such repair in the sole opinion of Beckman Coulter is minor, or unless such
modification is merely the installation of a new Beckman Coulter plug-in component for such
product.

PN B07711AA

B-1

Special Warranty for the Optima™ XE

Special Warranty

Special Drive Warranty

During the instrument warranty period (one year), there will be no charge for drive replacement if
the drive unit is installed, serviced, and operated in accordance with the conditions listed below.
During the drive’s second through tenth year of use there is a prorated drive replacement price
based on years of use if the drive unit is installed, serviced, and operated in accordance with the
conditions listed below.

Drive replacement price for units not under service contract = current drive exchange price

years of use

----------------------- -------------

x

〈〉labor and travel.+

10

NOTE

For details of drive coverage with a service contract, contact your local Beckman Coulter service

representative

Conditions

1. The drive has been operated only within its rated speed and temperature ranges.

2. The drive unit has not been subjected to unequal loading, improper rotor installation,

corrosion from material spilled onto the hub or accumulated in the chamber of the instrument.

3. The drive unit has not been disassembled, modified, or repaired, except by Beckman Coulter
personnel.

4. The drive unit was installed by a Beckman Coulter Field Service representative.

5. The instrument in which the drive unit has been used and operated, and its associated rotors,

were manufactured by Beckman Coulter and serviced only by Beckman Coulter Field Service
representatives.

If the above conditions are not met, the full appropriate exchange price for the drive will be
charged.

Disclaimer

IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF
FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER, INC.
SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSO­EVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR
REPLACEMENT OF THE PRODUCT.

B-2

PN B07711AA

Overview

APPENDIX C

Diagnostics

This section lists possible malfunctions and corrective actions. Maintenance procedures are
described in CHAPTER 6, Maintenance and Troubleshooting.

For any maintenance not covered in this manual, contact Beckman Coulter Field Service for
assistance. USA customers can call 1-800-742-2345. For international contacts, see the website at
www.beckmancoulter.com or use the contact numbers on the inside front cover of this book.

NOTE

It is your responsibility to decontaminate the ultracentrifuge, as well as any rotors and/or accessories,

before requesting service by Beckman Coulter Field Service.

Diagnostics/User Messages Chart

Refer to the chart below for a list of possible malfunctions and their corrective actions.

Table C.1 Diagnostics/User Messages Chart

Message Definition/Result Recommended Action

D100 - Turn off power
immediately

D101 - SBC
Communications

D102 - SCB
Communications

D103 - Inverter
Communications

Instrument cannot trip
breaker

Single board computer
boot error

Communications error Call Beckman Coulter Field Service.

Inverter I²C error Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

PN B07711AA

D104 - Inverter
Communications

D105 - Inverter
Communications

Inverter ADC I²C error ­U21

Inverter I²C I/0 Expander
error

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

C-1

Diagnostics

Diagnostics/User Messages Chart

Table C.1 Diagnostics/User Messages Chart (Continued)

Message Definition/Result Recommended Action

D107 - Inverter

TEM ADC I²C error - U24 Call Beckman Coulter Field Service.

Communications

D108 - Network
Communications

D109 - Network
Communications

D110 - Network
Communications

D111 - System Data
Error

D108 - Network

Be sure external devices are turned on.

Communications

Network error - ethernet

Be sure network cable is plugged in.

disconnected

Network error - cannot

Check to see if network is active.

renew IP address

Data error Note: If recovery fails, this condition will result in

a 200 minute delay before the diagnostic can be
cleared and the door opened. The power must
be left on, until the delay period is completed.

Call Beckman Coulter Field Service.

D112 - USB Port USB import/export error USB device may be full or removed too quickly.

Save again with another USB device..

D113 - Touch Screen
Not Detected

Touch Screen USB cable
not connected

Be sure USB touch screen cable is connected.

If problem persists, call Beckman Coulter Field
Service.

D114 - Code error Bad checksum on code

Call Beckman Coulter Field Service.

flash

D115 - Code error Corrupted data flash Note: This condition will result in a 200 minute

delay before the diagnostic can be cleared and
the door opened. The power must be left on,
until the delay period is completed.

Call Beckman Coulter Field Service.

A117 - Alert: Printer
error

No printer or printer driver Be sure printer is turned on and drivers are

installed.

If problem persists, call Beckman Coulter Field
Service.

A118 - Alert: Hard
drive error

D119 - SBC

Enhanced Write Filter

Call Beckman Coulter Field Service.

disabled

Unable to open serial port Call Beckman Coulter Field Service.

Communication

A120 - Alert: Simulator Simulator not found Call Beckman Coulter Field Service.

D121 - Internal

Software error Call Beckman Coulter Field Service.

software error

C-2

PN B07711AA

Table C.1 Diagnostics/User Messages Chart (Continued)

Message Definition/Result Recommended Action

Diagnostics

Diagnostics/User Messages Chart

C

A122 - Alert: Firmware
error

D123 - UI
Communications

A124 - Alert: Hard
drive space low

D125 - SBC
Communications

A200 - Alert: AC Power
Loss - Run continued

A201 - Alert: AC Power
Loss - Run stopped

D202 - Bus Current B us current measures z ero

D203 - Bus Voltage Bus voltage too high

Firmware error caused
reset

Communications error Call Beckman Coulter Field Service.

Hard drive is 90% full Backup files soon.

Communications
parameters are not
compatible

AC Power Loss - Run
continued

AC Power Loss - Run,
Delayed Run, or Program
stopped

(<0.1A)

(>220VDC) or Bus voltage
too low (<180VDC)

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

None

None

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

D204 - Power Supply Inverter +18V supply too

high (>19.8VDC)

D205 - Power Supply Inverter +18V supply too

low (<16.2VDC)

D206 - Power Supply Inverter +5V supply too

high (>5.5VDC)

D207 - Power Supply Inverter +5V supply too

low (<4.5VDC)

D208 - Power Supply Inverter -5V supply too

high (>-4.5VDC)

D209 - Power Supply Inverter -5V supply too low

(<-5.5VDC)

D210 - Power Supply SCB +12V supply too high

(>13.2VDC) or SCB +12V
supply too low
(<10.8VDC)

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

PN B07711AA

C-3

Diagnostics

Diagnostics/User Messages Chart

Table C.1 Diagnostics/User Messages Chart (Continued)

Message Definition/Result Recommended Action

D211 - Power Supply SCB +3.3V supply too high

(>3.63VDC)

D212 - Power Supply SCB analog +3.3V supply

too high (>3.63VDC) or
SCB analog +3.3V supply
too low (<2.97VDC)

D213 - Power Supply System +24V supply too

high (>26.4VDC) or
System +24V supply too
low (<21.6VDC)

D214 - Power Supply System +5V supply too

high (>5.5VDC)

D216 - AC Power Out of

AC Power Out of Range Check electrical power source.

Range

A217 - Alert: AC Power
Voltage Sag

AC Power Voltage Sag
below 180Vac

D300 - Rotor Speed Rotor speed exceeds

maximum rated speed

A301 - Alert: Rotor

Rotor set speed adjusted None

Speed Adjusted

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

None

Call Beckman Coulter Field Service.

A302 - Alert: Inertia

Inertia calibration failure Call Beckman Coulter Field Service.

Calibration

D303 - Speed Signals Overspeed timing signal is

<9 counts/revolution or
>47 counts/revolution, or
overspeed timing signal is
unstable

D304 - Speed Signals No tachometer signal

1. Check the condition of the rotor overspeed

disk.

2. Make sure the rotor is installed properly.

3. Verify the rotor load is within limits specified

in the rotor manual.

NOTE This condition will result in a 20-minute/

10K of RPM delay before the diagnostic can
be cleared and the door opened. The power
must be left on, until the delay period is
completed.

1. Make sure the rotor is installed properly.

2. Check the condition of the rotor overspeed

disk.

3. Verify the rotor load is within limits specified

in the rotor manual.

4. If the problem persists, call Beckman Coulter

Field Service.

C-4

PN B07711AA

Diagnostics/User Messages Chart

Table C.1 Diagnostics/User Messages Chart (Continued)

Message Definition/Result Recommended Action

D305 - Inertia Inertia check failure Be sure rotor is loaded properly.

Diagnostics

C

A400 - Alert: Vacuum
Calibration

A401 - Alert: Slow
Vacuum

D402 - Slow Vacuum Vacuum not <20 microns

D403 - Lost Vacuum Vacuum >750 microns for

Vacuum calibration failure Call Beckman Coulter Field Service.

Vacuum exceeds 750
microns after 4 minutes

after 20 minutes

1 minute after being <750
microns

1. Make sure door o-ring is clean, undamaged,

and properly lubricated.

2. Check for sample leakage. Clean and dry the

rotor chamber if needed.

3. If the problem persists, call Beckman Coulter

Field Service.

1. Make sure door o-ring is clean, undamaged,

and properly lubricated.

2. Check for sample leakage. Clean and dry the

rotor chamber if needed.

3. If the problem persists, call Beckman Coulter

Field Service.

1. Make sure door o-ring is clean, undamaged,

and properly lubricated.

2. Check for sample leakage. Clean and dry the

rotor chamber if needed.

3. If the problem persists, call Beckman Coulter

Field Service.

D404 - Lost Vacuum Va cuu m >50 m icr ons f or

10 minutes after being
<20 microns

D407 - Vacuum Vent Vacuum vent solenoid not

connected

D408 - Vacuum Vent Vacuum vent can not be

opened

D500 - Temperature
Control

D501 - Temperature
Control

D502 - Temperature
Control

Ambient thermistor open Call Beckman Coulter Field Service.

Ambient thermistor
shorted

Ambient temperature out
of range (<10°C or
>35°C)

1. Make sure door o-ring is clean, undamaged,

and properly lubricated.

2. Check for sample leakage. Clean and dry the

rotor chamber if needed.

3. If the problem persists, call Beckman Coulter

Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Adjust room temperature before operating.

PN B07711AA

C-5

Diagnostics

Diagnostics/User Messages Chart

Table C.1 Diagnostics/User Messages Chart (Continued)

Message Definition/Result Recommended Action

D503 - Temperature
Control

D504 - Temperature
Control

D505 - Temperature
Control

D506 - Temperature
Control

D507 - Temperature
Control

D508 - Temperature
Control

D509 - Temperature
Control

D510 - Temperature
Control

Can thermistor not

Call Beckman Coulter Field Service.

connected

Can thermistor shorted Call Beckman Coulter Field Service.

Can temperature out of

Call Beckman Coulter Field Service.

limits (<-30°C or >70°C)

TEM voltage too high Call Beckman Coulter Field Service.

TEM voltage too low Call Beckman Coulter Field Service.

TEM impedance too low Call Beckman Coulter Field Service.

TEM impedance too high Call Beckman Coulter Field Service.

Rotor temperature error

Call Beckman Coulter Field Service.
rate of change is not
decreasing and rotor
temperature is greater
than 10°C from set
temperature after running
for 15 minutes.

C-6

D512 - Temperature
Communications

D513 - Temperature
Communications

D514 - Temperature
Communications

D512 - Temperature
Communications

No communications - TEM
I²C

No communications ­thermopile

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

D600 - DriveDrive fault Drive fault Call Beckman Coulter Field Service.

D601 - Drive Bus current too high

Call Beckman Coulter Field Service.
(>30A)

D602 - Drive

Drive temperature >69°C Call Beckman Coulter Field Service.

Temperature

D603 - Damper Damper coil status error Call Beckman Coulter Field Service.

D604 - CF-32 Rotor Oil
Level

CF-32 oil level error Make sure the rotor oil level switch is installed on

the back panel for CF-32 operation. Add oil to

CF-32 rotor.

PN B07711AA