Beckman Coulter Optima MAX-XP Instructions For Use Manual

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Instructions For Use

Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.

Optima MAX-XP

Ultracentrifuge

393552AH August 2018

Optima MAX-XP

Beckman Coulter Eurocenter S.A. 22, rue Juste-Olivier Case Postale 1044 CH - 1260 Nyon 1, Switzerland Tel: +41 (0) 22 365 36 11

Ultracentrifuge

393552AH (August 2018)

Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

If you have any questions, contact our Customer Support Center.

• Worldwide, find us via our website at

www.beckman.com/support/technical.

• In the USA and Canada, call us at 1-800-369-0333.

• Outside of the USA and Canada, contact your local

Beckman Coulter Representative.

Find us on the World Wide Web at: www.beckman.com

Glossary of Symbols is available at beckman.com/techdocs (PN C24689).

Original Instructions

Revision History

Your Biomek i-Series documentation can be found on our website. For updates, go to

www.beckman.com/techdocs and download the latest version of the manual or system help for your

instrument.

Revision AG, 04/2017

Revision AH, 08/2018

CHAPTER 5, Supplies.

Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin of the amended page.

- Changes or additions were made to:Multiple Compliance.

- Changes or additions were made to: CHAPTER 4, User Message Chart;

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iii

Revision History

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Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate the ultracentrifuge. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter representative.

Alerts for Danger, Warning, Caution, and Note

DANGER

DANGER indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

Safety Notice

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury.

NOTE

NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

Safety During Installation and/or Maintenance

This ultracentrifuge is designed to be installed by a Beckman Coulter Field Service representative. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the ultracentrifuge.

This ultracentrifuge weighs 105 kg (230 lb). Do not attempt to lift or move it without assistance.

Any servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury. Make sure that the power switch is off and the ultracentrifuge is disconnected from the main power source, and refer such servicing to qualified personnel.

Do not replace any ultracentrifuge components with parts not specified for use on this ultracentrifuge.

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Safety Notice

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the ultracentrifuge to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the ultracentrifuge.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding a type of multiple-outlet

receptacle strip.

• Do not install the ultracentrifuge on a ground fault-protected power source.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into the ultracentrifuge and damage electrical or mechanical components.

Safety Against Risk of Fire

Fuses protect certain electrical circuits within this ultracentrifuge against overcurrent conditions. For continued protection against the risk of fire, replace only with the same type and rating specified.

This ultracentrifuge is not designed for use with materials capable of developing flammable or explosive vapors. Do not ultracentrifuge such materials (such as chloroform or ethyl alcohol) in this ultracentrifuge nor handle or store them within the required 30-cm (1-ft) area surrounding the ultracentrifuge.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the rotors and accessories designed for use in this ultracentrifuge.

• Do not exceed the maximum rated speed of the rotor in use.

• Do not lift or move the ultracentrifuge while the rotor is spinning.

• NEVER attempt to slow or stop the rotor by hand.

• NEVER attempt to override the door interlock system while the rotor is spinning.

• In the event of a power failure, do not attempt to retrieve the sample from the ultracentrifuge

for at least 1 hour. Then follow the instructions for sample recovery in

CHAPTER 4, Troubleshooting.

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Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Such materials should not be used in this ultracentrifuge, however, unless all necessary safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to their use.

• Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and C) and HIV (I-V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this ultracentrifuge without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual are handled; materials of a higher group require more than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health and safety guidelines.

Safety Notice

Chemical and Biological Safety

It is your responsibility to decontaminate the ultracentrifuge and accessories before requesting service by Beckman Coulter Field Service.

Summary of Instrument Labels

This section provides information for some labels and symbols appearing on the Optima MAX-XP instrument housing. These labels and symbols may be associated with user-serviceable procedures. Individual hazards associated with a specific procedure in this manual may use these labels and symbols, and are included in Warnings or Cautions within the procedures for that task.

Caution Symbol

This symbol indicates a caution message and appears adjacent to an explanation or other symbols that define the caution.

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Safety Notice

A28219-AA

Summary of Instrument Labels

RoHS Caution

This label and materials declaration table (the Table of Hazardous Substance's Name and Concentration) are to meet People's Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements.

This logo indicates that this electronic information product contains certain toxic or hazardous elements, and can be used safely during its environmental protection use period. The number in the middle of the logo indicates the environmental protection use period for the product. The outer circle indicates that the product can be recycled. The logo also signifies that the product should be recycled immediately after its environmental protection use period has expired. The date on the label indicates the date of manufacture.

Recycling Label

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE) Directive of the European Union. The presence of this marking on the product indicates:

• the device was put on the European Market after August 13, 2005 and

• the device is not to be disposed of via the municipal waste collection system of any member

state of the European Union.

It is very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing this label, please contact your dealer or local Beckman Coulter office for details on the take-back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.

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Multiple Compliance

• Recycling – Refer to the Recycling Label section in this document.

• – A “CE” mark indicates that a product has been assessed before being placed on the

market, and has been found to meet European Union safety, health, and/or environmental protection requirements.

• 169502 – This label indicates recognition by a Nationally Recognized Testing Laboratory (NRTL) that the instrument has met the relevant product safety standards.

Safety Notice

Summary of Instrument Labels

NOTE

• The RCM mark is intended for use on products that comply with Australian Communications

Media Authority (ACMA) EMC Requirements.

169502 is applicable to North American models only.

CAUTION Static Sensitive Area

Indicates an area of the instrument which is sensitive to static electrostatic discharge (ESD). To prevent damage due to electrostatic discharge, always wear a properly earth-grounded wrist strap while operating this instrument. For details on proper grounding, see IEEE standard P1100.

DANGER High Voltage

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Operation, replacement or servicing of any components where contact with bare, live hazardous parts could occur, possibly resulting in electric shock, should only be performed by your Beckman Coulter representative.

Safety Notice

Summary of Instrument Labels

Protective Ground

This symbol is used to indicate a protective ground. This instrument must be properly grounded. Do not under any circumstances operate the instrument unless it is properly grounded.

WARNING Risk of Fire

Before replacing fuses, shut off power and disconnect the power cord. Failure to do so can cause electric shock and/or equipment damage. Replace fuses only with approved type and rating replacement fuse.

Universal Serial Bus (USB)

This symbol indicates the location of a universal serial bus (USB) connector.

MAX-XP CAPACITY

This label indicates oil capacities for the indicated subsystems.

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WARNING 240 VAC

Safety Notice

Summary of Instrument Labels

This symbol indicates that the instrument has been configured for operation at 240 VAC. Consult with a Beckman Coulter service engineer for use at other line voltages.

NOTICE Regarding Vacuum Pump Oil

This label indicates to use only ULVAC R2 rotary vacuum pump oil (Beckman Coulter part number A51210).

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Safety Notice

Summary of Instrument Labels

Rotor Rotation

This indicates the direction of instrument rotor rotation.

SER NO (Serial Number)

SER NO

This label indicates the location of the instrument serial number.

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Revision History,iii

Safety Notice,v

Alerts for Danger, Warning, Caution, and Note,v

Safety During Installation and/or Maintenance,v

Electrical Safety,vi

Safety Against Risk of Fire,vi

Mechanical Safety,vi

Chemical and Biological Safety,vii

Summary of Instrument Labels,vii

Introduction,xix

CHAPTER 1:

Certification,xix

Scope of Manual,xix

Typographic Conventions,xx

CFC-Free Centrifugation,xx

Radio Interference,xx

Description,1-1

Intended Use,1-1 Rotor Chamber,1-2 Vacuum System,1-2

Temperature Sensing and Control,1-2

Drive,1-3

Controls and Indicators,1-3

Power Switch,1-3 Touchscreen Interface,1-3

Safety Features,1-6

Door,1-6 Barrier Ring,1-6 Imbalance Detector,1-6 Overspeed and Rotor Identification System,1-7

Name Rating Plate,1-7

Specifications,1-7

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Contents

Control Features,1-7 Physical Data,1-8

Available Rotors,1-9

CHAPTER 2:

CHAPTER 3:

Introduction,2-1

Space Requirements,2-1

Electrical Requirements,2-2

Introduction,3-1

Touchscreen Interface,3-1

Modes of Operation,3-2

Summary of Optima MAX-XP Run Procedures,3-3

Ultracentrifuge and Rotor Preparation,3-3 Manual Run,3-4 Programmed Run,3-5

Manual Operation,3-6

Preparing the Ultracentrifuge,3-6 Logging In,3-6 Selecting a Rotor,3-7 Entering Run Speed,3-8 Entering Run Time,3-9 Entering Run Temperature,3-15 Entering Acceleration and Deceleration Rates,3-16 Starting a Run,3-19 Stopping a Run,3-20 Viewing a Previous Run,3-21 Changing the Login Button Design,3-22

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Programmed Operation,3-23

Creating a New Program,3-24 Starting a Programmed Run,3-31 Making Changes to a Program,3-34 Deleting a Program,3-37

Pulse Run Operation,3-38

Remote Operation,3-40

Administrator Operations,3-40

Accessing Administrator Options,3-40 Set Interface Language,3-41 Setting the Date and Time,3-43 Adding and Managing Users,3-44 Setting User Login,3-49 Setting Rotor Logging,3-50 Populating the Rotor Library,3-50 Setting Audio Volume,3-54 Exporting Data,3-54

Contents

CHAPTER 4:

CHAPTER 5:

Introduction,4-1

User Messages,4-1

Retrieving Your Sample in Case of Power Failure,4-6

Circuit Breaker,4-9

Ultracentrifuge Care,5-1

Vacuum System,5-1 Drive Hub,5-2 Air-Intake and Exhaust Louvers,5-2

Cleaning,5-2

Ultracentrifuge Surfaces,5-2 Touchscreen Display,5-2

Decontamination,5-3

Sterilization and Disinfection,5-3

Storage and Transportation,5-3

Supply List,5-3

Supplies,5-4 Optional Accessories,5-4

Warranty for the Optima MAX-XP Ultracentrifuge

Illustrations

1.1 The Optima MAX-XP Ultracentrifuge,1-1

1.2 Temperature Control Diagram,1-3

1.3 Touchscreen Interface,1-4

2.1 Dimensions of the Optima MAX-XP Ultracentrifuge,2-2

3.1 Touchscreen Interface Main Screen,3-2

4.1 Example User Message on Touchscreen Interface,4-2

4.2 Removing the Top Cover of the Ultracentrifuge,4-7

4.3 Door Lock System,4-8

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Tables

3.1 Acceleration and Deceleration Rates,3-17

4.1 User Message Chart,4-2

Tables

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Tables

xviii

Certification

To ensure full system quality, the Beckman Coulter Optima MAX-XP Ultracentrifuge has been manufactured in a registered ISO 9001 or 13485 facility. It has been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies. Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com.

Introduction

Scope of Manual

This manual is designed to acquaint you with the Optima MAX-XP Ultracentrifuge, its functions, specifications, operation, and routine operator care and maintenance. We recommend that you read this entire manual, especially the Safety Notice and all safety-related information, before operating the ultracentrifuge or performing ultracentrifuge maintenance.

• CHAPTER 1 contains system specifications and a brief physical and functional description of the

ultracentrifuge, including the operating controls and indicators.

• CHAPTER 2 describes the space and power requirements for installing and connecting the

ultracentrifuge.

• CHAPTER 3 describes the ultracentrifuge operating procedures.

• CHAPTER 4 lists possible malfunctions with possible causes and suggested corrective actions.

• CHAPTER 5 contains procedures for routine operator care and maintenance, as well as a brief

list of supplies, replacement parts, and accessories.

CAUTION

If the ultracentrifuge is used in a manner other than that specified in this manual, the safety and performance of this equipment could be impaired. Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the appropriate rotor manual is the sole responsibility of the user.

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Introduction

Typographic Conventions

Certain symbols are used in this manual to call out safety-related and other important information. These international symbols may also be displayed on the ultracentrifuge and are reproduced on the inside back cover of this manual.

Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as buttons and displays.

• Touchscreen buttons (for example,

• Display names (for example,

CFC-Free Centrifugation

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the Optima MAX-XP Ultracentrifuge.

START

SPEED RPM

VACUUM

TIME hr:mm

) appear in boldface.

appear in boldface.

Radio Interference

This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of FCC rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with this instruction manual, may cause interference to radio communications. Operation of this equipment in a residential area is likely to cause interference, in which case the user will be required to correct the interference at his own expense.

Canadian Regulations

This equipment does not exceed the Class A limits for radio noise emissions from digital apparatus as set out in the radio interference regulations of the Canadian Department of Communications.

Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage radioelectrique édicté par le Ministère des Communications du Canada.

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Description

The Optima MAX-XP microprocessor-controlled tabletop ultracentrifuge (see Figure 1.1) generates high centrifugal forces for a variety of applications. The ultracentrifuge design features a variablefrequency induction drive, thermoelectric temperature control system, self-purging vacuum system, rotor overspeed identification system, user login feature, program memory that contains multiple five-step programs, and a choice of acceleration and deceleration rates.

Manual and programmed operations are available from the integrated touchscreen interface.

CHAPTER 1

Description

• In manual operation, you enter the individual run parameters before beginning each run.

• In programmed operation, you can duplicate runs quickly and accurately by selecting

Additionally, a pulse feature allows you to manually accelerate the rotor for sample preparation.

Figure 1.1 The Optima MAX-XP Ultracentrifuge

Intended Use

previously entered programs and running them again.

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Use the Optima MAX-XP Ultracentrifuge for applications requiring high force fields that are capable of separating and isolating small particles (virus, bacteria, and subcellular components like mitochondria) and large molecules (peptides, DNA, proteins). These samples are derived from a variety of natural and synthesized components.

1-1

Description

Temperature Sensing and Control

Rotor Chamber

The steel chamber is coated with a chemical-resistant finish to resist corrosion. The rotor drive hub and speed sensors are visible in the bottom of the chamber.

Vacuum System

A diffusion pump, in series with a mechanical vacuum pump, reduces chamber pressure to below 10 microns (1.34 Pa). The vacuum system turns on when you press the touchscreen, or when you start a run. When the vacuum system is turned on, a self-purging system continuously removes moisture from the vacuum system.

There are two ways to start the vacuum system (there must be power to the ultracentrifuge):

To start pumping air out of the chamber manually, press the

• The chamber door locks and the vacuum system starts.

VACUUM

button on the

button.

To start the vacuum system for a run, place the rotor in the chamber, close the door, and press

START

the

• The door locks, the rotor starts spinning, and the vacuum system starts.

• The

To release the vacuum system, press the complete stop.

• This unlocks the door and turns off the vacuum system, including the mechanical and

button.

VACUUM

activated. — The button displays the vacuum level in microns.

diffusion pumps.

button on the touchscreen interface turns green when the vacuum system is

Temperature Sensing and Control

The solid-state thermoelectric temperature control system uses only forced air—no coolant is required. With the power on, the temperature control system is activated when the door is closed. and the vacuum system is turned on. Run temperature can be set between 0 and 40°C.

VACUUM

button after the rotor has come to a

1-2

A sensor in the rotor chamber continuously monitors chamber temperature. The microprocessor calculates the required chamber temperature to maintain the selected rotor temperature. Peak-topeak fluctuations of rotor temperature (after reaching thermoequilibrium) will be within 0.1°C (see

Figure 1.2).

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Figure 1.2 Temperature Control Diagram

+2°

°C

-2°

Description

Drive

1. Actual rotor temperature

2. 0.1° (peak-to-peak fluctuation

Drive

The air-cooled, direct-drive induction motor is frequency controlled, with no gears or brushes. In addition, the drive does not require an oil vacuum seal or external oil reservoir. It is externally cooled by forced air and internally cooled by oil. The drive delivers ultra-smooth, quiet performance.

Controls and Indicators

Power Switch

The power switch, located on the right-hand side of the ultracentrifuge, controls electrical power to the ultracentrifuge. When the instrument is first turned on, the display will be blank for approximately 10 seconds until the user interface is launched. The power switch is also a circuit breaker that will trip to cut off power in the event of a power overload. The power switch must be turned on before the chamber door can be opened. In the event of a power failure, you can retrieve your sample manually. For more information, see Retrieving Your Sample in Case of Power Failure in

CHAPTER 4, Troubleshooting.

3. Time

4. Set Temperature

Touchscreen Interface

The operation of the ultracentrifuge is controlled via the touchscreen interface display, which comprises touch-sensitive display fields and buttons for entering and displaying run parameters and program information. When you press a button, additional screens may appear to allow you to enter or select more information. Figure 1.3 points out the elements of the touchscreen interface.

Use your fingertip to press the buttons on the touchscreen. A short beep sounds each time you press a button.

During operation (Run mode), the

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Speed, Time

, and

Te mp

display fields provide real-time status.

1-3

Description

Controls and Indicators

Figure 1.3 Touchscreen Interface

1. Windshield

2. Screen Instructions

3. Help Button

4. Set Values

5. Login Button

6. Buttons and Display Fields

7. Menu Button

The buttons and display fields on the Main screen are described briefly below. For complete information and instructions on using the touchscreen interface, see CHAPTER 3, Operation.

Color-Coded Windshield

The windshield changes color to indicate the current state of the ultracentrifuge:

• Blue indicates Set-up mode.

• Green indicates that a run (or a delayed run) is in progress (Run mode).

• Red indicates a diagnostic or user alert. A message is displayed to provide information and

allow you to take the appropriate action.

The center of the windshield displays instructions for entering parameters and other commands in each screen.

MENU BACK

Press the working in other screens, the

button in the upper left-hand corner of the screen to open the menu. When you are

button changes to the

BACK

button to allow you to navigate

back to the previous screen.

1-4

The menu options are:

History

•

Accel/Decel

— Displays a list of previous runs.

— Displays a screen in which to select the acceleration and deceleration rates.

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Controls and Indicators

•

Program

— Displays a list of programmed runs and provides an interface from which to set up

new programs.

•

Admin

— Opens the

logged in, opens the

My Options

•

displays the

Pulse

•

— Activates pulse mode.

— If no users are logged in, opens the

Choose Button Type

Administrator Options

User Login

screen.

screen. If no user with Administrator privileges is

User Login

screen. If a user is logged in,

HELP

Press the

button in the upper right-hand corner of the screen to display an online help

window. The help text that appears applies to the information in the current screen.

ROTOR

Press the

ROTOR

button to select a rotor. The currently selected rotor appears on the button.

RPM/RCF

Press the

RPM/RCF

button to toggle between RPM and RCF.

Description

RPM indicates rotor speed in revolutions per minute. RCF (relative centrifugal field) represents the ratio of the centrifugal acceleration at a specified radius and speed to the standard acceleration of gravity.

The currently selected mode appears in green above the horizontal line on the button.

SPEED

Press the

button to select the speed. The set speed appears above this button, which doubles as a display field for the actual run speed. The set speed and actual speed can be displayed in RPM or RCF.

When the speed is increasing (rotor acceleration), animated arrows in the display field point upward. When the speed is decreasing (rotor deceleration), the animated arrows point downward.

TIME

Press the

button to select the run duration in hours and minutes. This button doubles as a

display field for the actual run time as follows:

• In a timed run, this display field indicates the remaining run time in hours and minutes. The

START

time display begins counting down when you press the

button; it continues counting down to 0. Animated arrows will point downward. The run automatically ends when the set time reaches 0 and deceleration begins (a tone sounds when the rotor stops spinning). If a malfunction shuts down the ultracentrifuge during a run, the countdown stops and the time that remained when the run stopped stays in the display. By comparing this time with the original set time, you can determine when the run ended.

• In a hold run, the time display indicates the time elapsed since you pressed the

START

button.

Animated arrows will point upward. After 99 hours and 59 minutes, the timer resets to 0 and

HOLD

continues counting elapsed time. The word “

• In a delayed run, when you press the

START

button after setting the start date and/or time, the

” displays as the set time.

time display first reflects the remaining delay time. When the delay concludes and the run starts, the time display indicates the remaining run time.

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• In a pulse (momentary) run, the time display is blank.

1-5

Description

Safety Features

TEMP

Press the temperature appears above this button, which doubles as a display field for the actual run temperature.

VACUUM

Press the

Press the appears on this button.

The touchscreen interface includes a user timeout function. The currently logged-in user will be logged out automatically after 5 minutes of inactivity: 1) while setting up parameters for a run, 2) if Pulse mode is selected but the Pulse run is not started, and 3) after the user’s run has been completed.

START STOP

Press the

TEMP

button to select the rotor temperature in degrees C at thermoequilibrium. The set

VACUUM

START

button to start or stop the vacuum system.

button to go to the

User Login

screen. The name of the currently logged-in user

button to start a run. To stop a run, press the

STOP

button.

Safety Features

The Optima MAX-XP ultracentrifuge has been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft). Ultracentrifuge safety features are described below.

Door

The steel chamber door has an electromechanical door-locking mechanism to prevent operator contact with a spinning rotor. To lock and unlock the door, press the touchscreen.

When there is a power failure, the door lock can be tripped manually for sample recovery. See

CHAPTER 4, Troubleshooting.

Barrier Ring

A structural steel armor ring acts as the primary barrier, surrounded by a steel secondary barrier ring to provide full protection for the operator.

Imbalance Detector

VACUUM

button on the

1-6

An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor loads are severely out of balance. At low speeds, an incorrectly loaded rotor can cause an imbalance. Rotor instability can also occur if the ultracentrifuge is moved, or if it is not resting level on the work surface. See CHAPTER 4, Troubleshooting.

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Overspeed and Rotor Identification System

The overspeed system, which includes magnetic speed sensors in the rotor chamber and magnets on the bottom of each rotor, continuously monitors the rotor during centrifugation. At 1000 RPM, the system identifies the maximum permitted speed.

Name Rating Plate

A name rating plate is affixed to the rear of the ultracentrifuge. When contacting Beckman Coulter regarding your ultracentrifuge, always mention the serial number and model number. You can also view the serial number and other information by pressing the

ABOUT

button in the Help screen.

Specifications

Name Rating Plate

HELP

button, then pressing the

Description

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee.

Control Features

Specifications Description

Speed • Set speed:

Time • Set time:

— 5000 to 150,000 RPM in 1000-RPM increments

• Speed display: — actual rotor speed in 100-RPM increments above 5000 RPM and

10-RPM increments at speeds below 5000 RPM

• Speed control: — actual rotor speed, ±50 RPM of set speed

— to 99 hours 59 minutes

• Time display: — Timed run: indicates run time remaining — Hold mode: indicates actual run time — Programmed operation: indicates time remaining in step

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1-7

Description

Specifications

Specifications Description

Te mp er a t ur e • Set temperature:

— 0 to 40°C in 1° increments

• Temperature control (after equilibration): — ±2°C of set temperature

• Temperature display: — actual rotor temperature in 0.1° increments

• Ambient temperature range: — 15 to 35°C

Acceleration 10 acceleration profiles

• 9 slow rates from 0 to 5000 RPM followed by full acceleration to set speed; or maximum acceleration

Deceleration 11 deceleration profiles

• 10 slow rates from 5000 to 0 RPM, including coasting to a stop without brake; or full dynamic braking from set speed

Physical Data

Specification Description

Width 73.9 cm (29.1 in.)

Depth 61.7 cm (24.3 in.)

Height • Front left: 34.5 cm (13.6 in.)

Weight 105 kg (230 lb)

Ventilation Clearances (sides and rear)

Finishes • Touchscreen panel:

Electrical Supply Class 1

• Rear right: 39.4 cm (15.5 in.)

7.6 cm (3.0 in.)

— coated polycarbonate

• Top s u r f a ce : — acrylic baking enamel

• Other surfaces: — general purpose paint

1-8

Vacuum below 10 microns (1.34 Pa)

Electrical Requirements • 220/240 VAC~, 6 A, 50 Hz

• 120 VAC~, 12 A, 50/60 Hz

• 100 VAC~, 12 A, 50/60 Hz

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Specification Description

Humidity Restrictions <75% (noncondensing)

Description

Available Rotors

Maximum Heat Dissipation into Room Under Steady-State Conditions

Noise Level 1 m in front of ultracentrifuge

Installation (overvoltage) Category

Pollution Degree

a. Normally, only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation

must be expected.

Available Rotors

All Beckman Coulter ML- and TL-series rotors can be used in the Optima MAX-XP ultracentrifuge. The rotors are described in individual manuals that accompany each rotor. Information on rotors and accessories is also available in Rotors and Tubes for Tabletop Preparative Ultracentrifuges (TLR-IM) and in the Beckman Coulter Ultracentrifuge Rotors, Tubes & Accessories catalog (publication BR-8101).

2400 Btu/hr (0.7 kW)

<47 dB(A)

Rotor Profile Description

MLA-150 Fixed Angle 30° Angle

MLA-130 Fixed Angle 28° Angle

TLN-120 Near Vertical Tube 8° Angle

Max RPM/

k factor

150,000

10.4

130,000

8.7

120,000

Number of

Max RCFb (× g)

at r

max

1,003,000 8 × 2.0 mL 393554

1,019,000 10 × 2.0 mL TL-TB-021

585,000 8 × 1.2 mL TL-TB-017

Tub es × Nominal

Capacity

Rotor

Manual

Number

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1-9

Description

Available Rotors

Rotor Profile Description

TLA-120.2 Fixed Angle 30° Angle

TLA-120.1 Fixed Angle 30° Angle

TLA-110 Fixed Angle 28° Angle

TLN-100 Near Vertical Tube 9° Angle

Max RPM/

k factor

120,000

110,000

100,000

Max RCFb (× g)

at r

627,000 10 × 2.0 mL TL-TB-016

627,000 14 × 0.5 mL TL-TB-015

657,000 8 × 5.1 mL TL-TB-019

450,000 8 × 3.9 mL TL-TB-013

max

Number of

Tub es × Nominal

Capacity

Rotor

Manual

Number

TLA-100.4 Fixed Angle 28° Angle

TLA-100.3 Fixed Angle 30° Angle

TLA-100.2 Fixed Angle 30° Angle

TLA-100.1 Fixed Angle 30° Angle

100,000

543,000 8 × 5.1 mL TL-TB-014

100,000

543,000 6 × 3.5 mL TL-TB-011

100,000

436,000 10 × 2.0 mL TL-TB-005

100,000

436,000 12 × 0.5 mL TL-TB-004

1-10

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Description

Available Rotors

Rotor Profile Description

TLA-100 Fixed Angle 30° Angle

TLV-100 Vert i c a l Tub e 0° Angle

MLN-80 Near Vertical Tube 9° Angle

MLA-80 Fixed Angle 26° Angle

Max RPM/

k factor

100,000

80,000

Max RCFb (× g)

at r

436,000 20 × 0.2 mL TL-TB-003

400,000 8 × 2.0 mL TL-TB-007

390,000 8 × 8.0 mL TL-TB-022

444,000 8 × 8.0 mL TL-TB-024

max

Number of

Tub es × Nominal

Capacity

Rotor

Manual

Number

MLA-55 Fixed Angle 35° Angle

TLA-55 Fixed Angle 45° Angle

TLS-55 Swinging Bucket

90° Angle

MLS-50 Swinging Bucket

90° Angle

55,000

50,000

287,000 8 × 13.5 mL TL-TB-026

186,000 12 × 1.5 mL TL-TB-020

259,000 4 × 2.2 mL TL-TB-006

268,000 4 × 5.0 mL TL-TB-023

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1-11

Description

Available Rotors

Rotor Profile Description

MLA-50 Fixed Angle

Max RPM/

k factor

50,000

Max RCFb (× g)

at r

233,000 6 × 32.4 mL B03896

max

Number of

Tub es × Nominal

Capacity

Rotor

Manual

Number

30° Angle

TLA-45 Fixed Angle

45,000

125,000 12 × 1.5 mL TL-TB-012

45° Angle

a. Maximum speeds are based on a solution density of 1.7 g/mL for all rotors, except the MLS-50, MLA-50, MLA-55, and MLA-80;

solution density for these rotors is 1.2 g/mL. The k factors are listed for all Beckman Coulter rotors (using the largest-volume tube) as a measure of the rotor’s relative pelleting efficiency.

b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rω

standard acceleration of gravity (g) according to the following formula: RCF = rω ω is the angular velocity in radians per second (2 π RPM /60), and g is the standard acceleration of gravity (9807 mm/s After substitution: RCF = 1.12r (RPM/1000)

c. No longer manufactured.

d. At speed.

e. Temperature control at set temperature is 0 to 35°.

/g — where r is the radius in millimeters,

) to the

1-12

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Introduction

Preinstallation requirements have been provided for your Optima MAX-XP ultracentrifuge. The following information is included in case the ultracentrifuge must be relocated.

CHAPTER 2 Preinstallation Requirements

NOTE

This ultracentrifuge is designed to be installed by Beckman Coulter Field Service. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the ultracentrifuge.

Space Requirements

WARNING

Do not place the ultracentrifuge near areas containing flammable reagents or combustible fluids. Vapors from these materials could enter the ultracentrifuge air system and be ignited by the motor. Maintain a 30-cm (1-ft) clearance envelope around the ultracentrifuge while it is running. No persons or any hazardous materials should be within this clearance boundary while the ultracentrifuge is operating, except to change operating controls, if required.

If it is necessary to move the ultracentrifuge, maintain the following conditions:

Select a location away from heat-producing laboratory equipment, with sufficient ventilation to allow heat dissipation.

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Position the ultracentrifuge on a level surface, such as a sturdy table or laboratory bench that can support the weight of the ultracentrifuge 105 kg (230 lb) and resist vibration.

a. Place the ultracentrifuge at least 5.1 cm (2 in.) from the front edge of the laboratory bench.

2-1

Preinstallation Requirements

7. 6

3. 0

in.

7. 6

3. 0

73. 9

29.1

61.7

24.3

39.4

15.5

34.5

13.6

Electrical Requirements

In addition to space for the ultracentrifuge itself (see Figure 2.1 for dimensions), allow 7.6 cm (3-in.) clearances at the sides and back to ensure sufficient air circulation.

The ultracentrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during operation.

Relative humidity should not exceed 75% (noncondensing).

Figure 2.1 Dimensions of the Optima MAX-XP Ultracentrifuge

Electrical Requirements

Voltage ranges 220/240 VAC~, 6 A, 50 Hz

120 VAC~, 12 A, 50/60 Hz 100 VAC~, 12 A, 50/60 Hz

To reduce the risk of electrical shock, this ultracentrifuge uses a 1.83-m (6-ft) three-wire electrical cord to be attached to the IEC 320/CEE-20 AC power connector at the rear of the ultracentrifuge and a plug to connect to earth-ground. (A plug that meets your local electrical and safety requirements was supplied with the ultracentrifuge. Contact your local Beckman Coulter office for specific information regarding local requirements.) To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Verify that the line voltage agrees with the voltage listed on the name rating plate affixed to the ultracentrifuge. Then plug in both ends of the ultracentrifuge power cord.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet

receptacle strip.

2-2

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Preinstallation Requirements

Electrical Requirements

• If there is any question about voltage, have a qualified service person measure it under load while the drive is operating.

To ensure optimal safety, the ultracentrifuge should be wired to a remote emergency switch (preferably outside the room where the ultracentrifuge is housed, or adjacent to the exit from that room). In case of a malfunction, the ultracentrifuge can be disconnected from the main power source.

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2-3

Preinstallation Requirements

Electrical Requirements

2-4

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Introduction

This section contains manual and programmed operating procedures. A summary is provided at the start of this section for experienced users.

CHAPTER 3 Operation

WARNING

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and C) and HIV (I-V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this ultracentrifuge without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

WARNING

Do not use the ultracentrifuge in the vicinity of flammable liquids or vapors, and do not run such materials in the ultracentrifuge. Do not lean on the ultracentrifuge or place items on it while it is operating.

Touchscreen Interface

The touchscreen interface (see Figure 3.1) comprises menu options and touch-sensitive display fields and buttons for entering and displaying run parameters and program information. When you press a button or select a menu option, additional screens appear to allow you to enter or select more information.

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3-1

Operation

Touchscreen Interface

You may configure the ultracentrifuge touchscreen for user interaction with different languages. See Set Interface Language.

Use your fingertip to press the buttons on the touchscreen.

A short beep sounds each time you press a button on the touchscreen (unless the audio volume has been muted by your administrator).

Figure 3.1 Touchscreen Interface Main Screen

Modes of Operation

Setup Mode

During Setup mode, the ultracentrifuge is available for setting a run. The windshield is blue.

The touchscreen interface includes a user timeout function. The current logged-in user will be logged out automatically after 5 minutes of inactivity:

1. while setting up parameters for a run,

2. if Pulse mode is selected but the Pulse run is not started,

3. after the user’s run has been completed.

Run Mode

During Run mode, the windshield is green to indicate that a run is in progress. These parameters can be changed during Run mode: speed, time, temperature, and Accel/Decel; however, if the run is above 5000 RPM, the new Accel value will be ignored and the new Decel value will be used.

Some Administrator functions can be performed in Run mode. For more information, see

Administrator Operations.

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Diagnostics/User Messages

When a condition arises that requires operator attention, the windshield turns red. Press the windshield to open a dialog showing the diagnostic message. User messages communicate information about the ultracentrifuge or alert you to an abnormal condition. For more information, see CHAPTER 4, Troubleshooting.

Summary of Optima MAX-XP Run Procedures

This section provides a quick view of the steps for running the ultracentrifuge both manually and via a program. This is provided as a reference after you become are familiar with using the ultracentrifuge and the touchscreen interface. For detailed procedures that include images of the interface screens, see Manual Operation, Programmed Operation, and Pulse Run Operation.

For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. For non-room temperature runs, prepare the system as described under Ultracentrifuge Pre-Run Cooling

or Warming.

Operation

Summary of Optima MAX-XP Run Procedures

Ultracentrifuge and Rotor Preparation

Prepare the rotor for centrifugation as described in the applicable rotor manual.

NOTE

Installing the Rotor

The power must always be turned on before you can unlock and open the chamber door.

To achieve optimum ultracentrifuge performance, follow these instructions between runs: Leave the ultracentrifuge powered on, the door closed, and the vacuum turned on. You do not need to leave a rotor inside the chamber.

Turn the power on.

• The touchscreen interface display turns on and is available.

a. To end a run for any reason, do not turn the power off.

STOP

b. Press the

Try opening the door.

• The ultracentrifuge will accept this command only when the rotor is at rest.

button.

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a. If the chamber is under vacuum and the door is locked, press the

the chamber and unlock the door.

• You can hear a slight hissing sound when the chamber vents.

After the chamber has been vented, use the door handle to slide the door open.

VACUUM

button to vent

3-3

Operation

Summary of Optima MAX-XP Run Procedures

Install the rotor according to the directions in the rotor manual.

a. Ensure that the rotor is seated on the drive hub.

Close the chamber door.

a. To keep the chamber clean and dry, leave the door closed whenever possible.

Ultracentrifuge Pre-Run Cooling or Warming

Follow these steps to precool or warm the ultracentrifuge.

Press the

TEMP

button on the Main screen.

Manual Run

• The

Enter the temperature, and press the OK button to accept.

• The entered temperature becomes the set temperature that appears above the

Close the door.

Press the

• Note that it is not necessary to use the

• Pre-run cooling or warming begins.

NOTE

Enter Run Temperature

button on the Main screen.

VACUUM

In addition to precooling or warming the ultracentrifuge, cool or warm the rotor outside the

ultracentrifuge to the required temperature before the run for fast temperature equilibration.

button to turn the vacuum system on.

screen appears.

START

button.

TEMP

3-4

This section is provided as a quick reference for executing a manual run. For detailed procedures of each step accompanied by screen shots of the touchscreen interface, see Manual Operation.

Turn the power on.

Install the rotor according to the applicable rotor manual, then close the chamber door.

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Summary of Optima MAX-XP Run Procedures

Press the rotor list.

• This step is optional.

a. If you don’t want to select a rotor, go to Step 4.

Press the

To accept the default Accel/Decel rates of Max (maximum), go to Step 8.

ROTOR

button, then choose the rotor (listed by type and serial number) from the

SPEED

button, then enter the run speed (5000 to 150,000 RPM).

TIME

button, then enter the run time (up to 99 hours, 59 minutes).

TEMPERATURE

button, then enter the required run temperature (0 to 40°C).

Operation

• This step is optional.

a. Select the

(fastest) to 9 (slowest).

b. Select the deceleration rate number, from Max (fastest) to 0 (coast).

• You can skip this step and accept the default rates of Max (maximum).

Press the

Programmed Run

This section is provided as a quick reference for executing a programmed run. This procedure assumes that programmed runs have been created and saved. For detailed procedures of each step accompanied by screen shots of the touchscreen interface, see Programmed Operation.

Turn the

Install the rotor according to the applicable rotor manual, then close the chamber door.

Accel/Decel

START

button to start the run.

POWER

switch to ON.

menu option, and select the acceleration rate number, from Max

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Select the

Program

menu option, then select a program from the list.

3-5

Operation

Manual Operation

Press the OK button to load the program parameters.

Press the

START

button to start the run.

Manual Operation

This section includes detailed procedures for entering run parameters for manual operation.

Preparing the Ultracentrifuge

In the first step of a manual run, install the rotor and perform any precooling or prewarming procedures that may be required. For more information and detailed steps, see Ultracentrifuge and

Rotor Preparation.

Logging In

Depending on your particular setup, you may need to log in with your user name. If your Administrator enables prior to the start of a run.

Each user name in the system is accompanied by a PIN. To obtain your PIN, check with your Administrator.

To log in prior to performing any other actions, press the

• The

User Login

screen appears.

is turned on by your Administrator, you will be required to log in

button on the Main screen.

3-6

a. If you have just pressed the

automatically, go to Step 2.

START

button to start a run and the

User Login

screen appears

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Select your user name, enter your PIN on the keypad, and press OK to accept.

• The Main screen returns to view.

• If you were required to log in, the run starts.

Selecting a Rotor

There are two reasons for selecting a rotor:

Operation

Manual Operation

• To track rotor usage (rotor logging). If your Administrator has turned must select a rotor prior to performing a run.

• To convert RPM to RCF. The ultracentrifuge needs to know the r

of the rotor on which to

max

perform its calculations.

On the Main screen, press the

• The

Choose Rotor

screen appears.

ROTOR

button.

a. If necessary, use the large arrows to bring additional rotor names into view.

Rotor Logging

on, you

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NOTE

If you are starting a run without selecting a rotor and a message appears asking you to select a

rotor, press

If is selected and you want to remove it from your run parameters, select another rotor.

in the message box, then press the

No Rotor Selected

ROTOR

button.

is highlighted in this list, it means that no rotor is currently selected. If a rotor

No Rotor Selected

, or select

3-7

Operation

Manual Operation

Select the desired rotor, and press the OK button to accept.

• The Main screen returns to view, and the selected rotor name and serial number appear on

ROTOR

the

button.

a. If you were about to start a run and were required to select a rotor, the run starts

automatically when the Main screen returns to view.

To convert RPM to RCF for the selected rotor, press the

• The conversion is calculated automatically, and the set RCF value appears above the

Speed

— “

Entering Run Speed

Run speed ranges from 5000 to 150,000 RPM. If no new speed is entered, the ultracentrifuge automatically selects the last entered speed. The last three digits of the run speed are fixed as zeros and cannot be changed. For example, to enter a run speed of 100,000 RPM, type 100 on the keypad display.

NOTE

To enter or view an RCF value, you must select the rotor first. For information on selecting a rotor, see

Selecting a Rotor.

RPM/RCF

display.

RCF

” displays in green above the horizontal line on the

button.

RPM/RCF

button.

3-8

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On the Main screen, press the

SPEED

button.

Operation

Manual Operation

• The

• The acceptable values for speed are shown above the display field: from 5000 to

• If a rotor is selected, the display will show the speed range for that rotor.

Enter the speed on the keypad display, and press the OK button to accept.

• The Main screen returns to view, and the set speed appears above the

Enter Run Speed

150,000 RPM.

— For example, 5000 to 80,000 is the acceptable speed range for the MLA-80.

screen appears.

Speed

display.

NOTE

grayed out. If you have selected a rotor, any value above the maximum speed for the rotor will cause the

To change to the new allowable run speed for the selected rotor at any time during a manual run, repeat Steps 1 and 2.

• The rotor will accelerate or decelerate to the new speed if a run is in process.

Entering Run Time

There are three different run time modes:

• In Timed mode, the time display begins counting down when the rotor begins to spin. The run time automatically terminates when the set time reaches zero.

• In Hold mode, the time display counts up until you stop the run.

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If you enter an RPM value greater than 150,000 and no rotor is selected, the OK button will be

button to be grayed out

3-9

Operation

Manual Operation

• In Delayed run mode, the time display first shows the remaining delay time. When the delay has

Timed Mode

Run time can be set for up to 99 hours and 59 minutes. If no new run time is entered, the ultracentrifuge automatically selects the last entered run time. The Time display begins counting down when the rotor starts to spin. The run automatically terminates and the rotor begins to decelerate when the set time reaches zero. A tone sounds when the rotor has stopped (unless the audio volume has been muted by your administrator).

concluded, the run starts and the time display counts down as in Timed mode.

On the Main screen, press the

TIME

button.

• The

• The acceptable values for run duration are shown above the display field: from 00:01 to

Enter the time on the keypad display, and press the OK button to accept.

• The Main screen returns to view, and the set time appears above the

Enter Run Duration

99:59 HH:MM.

screen appears.

Time

display.

3-10

• If you enter a number of minutes greater than 59, the ultracentrifuge automatically recalculates the time in hours and minutes.

To change the set time at any time during a manual run, repeat Steps 1 and 2.

• The ultracentrifuge will adjust to the new run duration.

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Hold Mode

Operation

Manual Operation

In Hold mode, the run continues until you stop the run by pressing the

NOTE

During Hold mode, the time display starts at zero and continues counting up until you stop the run. If

the count reaches 99:59, it resets to 0.

On the Main screen, press the

• The

• The acceptable values for run duration are shown above the display field: from 00:01 to

Enter Run Duration

99:59 HH:MM.

TIME

button.

screen appears.

STOP

button.

Press the

• The

Press the OK button.

• The Main screen returns to view, and Hold mode is indicated above the

HOLD

button.

HOLD

button turns green, and the word

HOLD

appears in the

Time

display field.

Time

display.

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3-11

Operation

Manual Operation

Setting Up a Delayed Run

You can set up a run that starts or stops in the future.

To cancel Hold mode, repeat steps 1–3.

HOLD

• The

—

On the Main screen, press the

button turns gray in the

HOLD

disappears from above the

TIME

button.

Enter Run Duration

Time

Display on the Main screen.

screen.

• The

Enter the run duration, and press the

• The

Enter Run Duration

Set Run Delay

screen appears.

DELAYED RUN

button.

3-12

• The current date and time display in the fields.

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To set a delayed run to start on a particular date and/or time, press the

Date

and

Time

• The

fields become active so you can change them.

Manual Operation

START AT

Operation

button.

a. To set a delayed run to stop on a particular date and/or time, press the

NOTE

Press the large arrows until you reach the desired date and time.

a. To change the date or time rapidly, hold down the corresponding arrow.

Press the OK button to accept.

• The

If you set up a run to stop at a particular time, the starting time will be calculated

automatically by the ultracentrifuge.

Enter Run Duration

screen returns to view; the

DELAYED RUN

STOP AT

button is green.

button.

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3-13

Operation

Manual Operation

Press the OK button to accept.

• The Main screen returns to view, and the set duration of the delayed run appears above the

Time

display.

Press the

START

button.

• The Main screen indicates that a delayed run is in progress.

time

• The

• When the delay concludes and the run starts, the

display first reflects the remaining delay time (as shown in the example below).

time

display indicates the remaining

run time.

a. If you are not logged in and

User Login

has been turned on by the Administrator, you are

required to log in.

• For more information, see Logging In.

b. If you are starting a run without selecting a rotor and a message appears asking you to

select a rotor, press

in the message box.

• You will need to select a rotor.

• For more information, see Selecting a Rotor.

NOTE

During a delayed run, you cannot change any parameters.

3-14

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To manually stop the delayed run at any time, press the

• Any time a run is stopping, it is indicated on the Main screen.

To cancel the delayed run:

STOP

button.

Operation

Manual Operation

a. Press the b. Press the c. Press the d. Press OK in the

TIME

DELAYED RUN

NO DELAY

Entering Run Temperature

Run temperature can be set from 0 to 40°C. If no new value is entered, the ultracentrifuge automatically uses the last entered temperature. If you clear the previous entry, 25°C is used as the operating temperature.

button in the Main screen.

button in the

Enter Run Duration

Set Run Delay

screen to return to the Main screen.

screen.

screen, and press OK.

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3-15

Operation

Manual Operation

On the Main screen, press the

TEMP

button.

• The

• The acceptable values for run temperature are shown above the display field: from

Enter the temperature on the keypad, and press the OK button to accept.

• The Main screen returns to view, and the set run temperature appears above the

Enter Run Temperature

0to40°C.

Te mp

display.

screen appears.

NOTE

To change the set temperature at any time during a manual run, repeat Steps 1 and 2.

• The ultracentrifuge accepts the new temperature immediately.

NOTE

If you enter a temperature value greater than 40, the OK button will be grayed out.

If the rotor temperature is not within 3°C above the set temperature for more than 40 minutes,

a diagnostic message appears. If a run is in progress, the rotor will decelerate to a stop. See

CHAPTER 4, Troubleshooting

Entering Acceleration and Deceleration Rates

The ultracentrifuge provides ten acceleration rates and eleven deceleration rates to protect the gradient and sample-to-gradient interface. Table 3.1 lists these rates by their corresponding numbers on the touchscreen interface. Acceleration time is the time it takes a rotor to reach 5000 RPM from rest. At 5000 RPM, maximum acceleration takes over until the rotor reaches set speed. Deceleration time is the time it takes a rotor to decelerate from 5000 to rest. From set speed to 5000, the rotor decelerates with full dynamic braking.

3-16

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Tab le 3.1 Acceleration and Deceleration Rates

Operation

Manual Operation

Touchscreen

Number

Max

10:30 1:00

21:00 1:30

31:30 2:00

42:00 2:30

52:30 3:00

63:00 4:00

73:30 6:00

84:00 8:00

95:00 10:00

0 N/A Coasting stop from set speed

a. Maximum rate. If no touchscreen number is selected, the rotor will accelerate and/or decelerate at

the maximum rates.

from 0 to 5000 RPM (MM:SS)

ACCEL Time

0:15

DECEL Time

from 5000 to 0 RPM (MM:SS)

0:15

without braking

If you use the default rates of maximum, the ultracentrifuge automatically accelerates and decelerates at maximum rate. When you change either the acceleration or deceleration rate,

Decel

and change the rates using these buttons as an alternative to using the

buttons display appear on the Main screen showing the selected rates. You can then

Accel/Decel

menu option.

Accel

On the Main screen, press the

• The list of menu options appears.

button in the upper left-hand corner.

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3-17

Operation

Manual Operation

Select

Accel/Decel

• The

Select Accel/Decel Rates

screen appears.

— The default value for both is Max (maximum speed).

Press the desired rate(s) by pressing the appropriate number on the sliders.

• The time duration for the rate you select is displayed in the fields on the right.

Coast

— If you select a deceleration rate of Coast, “

” appears, as shown in this example.

3-18

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Press the OK button to accept.

• If either rate is set to anything other than Max, Main screen showing the selected rates.

ACCEL

and

Manual Operation

DECEL

buttons appear on the

Operation

Starting a Run

On the Main screen, press the

• The rotor starts spinning.

• The vacuum system turns on unless it was previously turned on.

• The touchscreen windshield turns green.

• Animated arrows in the

• The

START

button.

— The

ultracentrifuge’s progress until the set values are reached.

VACUUM

START

button turns green and the vacuum level is displayed.

Speed, Time,

button changes to the

STOP

and

button.

Te mp

display fields represent the

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• The run will end automatically when the will sound.

Time

display counts down to zero, and a tone

3-19

Operation

Manual Operation

a. If you are not logged in and

b. If you start a run without selecting a rotor and a message appears asking you to select a

NOTE

After the run has concluded, you can repeat the run using the same parameters. To do this, press the

• The set parameters will be used for the next run.

NOTE

Stopping a Run

User Login

required to log in.

• For more information, see Logging In.

rotor, press

• You’ll need to select a rotor.

• For more information, see Selecting a Rotor.

Some features are not accessible during a run.

Keep the chamber door closed between runs.

START

in the message box.

button.

has been turned on by the Administrator, you are

If you want to stop a run manually, press the

• Any time a run is stopping, it is indicated on the Main screen.

When the run stops and the time reaches 0, you can press the vacuum system and vent the vacuum.

• The chamber door unlocks so you can open it and remove your sample.

STOP

button on the Main screen.

VACUUM

button to turn off the

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Viewing a Previous Run

Viewing a previous run allows access to previous set and actual run information.

On the Main screen, press the

• The list of menu options appears.

button.

Operation

Manual Operation

Select

History

• A list of previous runs is displayed.

If necessary, use the arrows to bring additional runs into view.

a. Select the desired run from the list.

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3-21

Operation

Manual Operation

To view the details of the selected run, press the

VIEW DETAILS

button.

• The

a. Use the large arrows to bring more details into view.

• If you are viewing a programmed run that contains more than one step, the details for each

Press the

History Details

step appear on a separate screen.

BACK

button to return to the list of previous runs.

screen appears and displays complete information on the run.

Changing the Login Button Design

The name of the current logged-in user is displayed on the can customize the appearance of this button. Twelve designs are available for selection.

Verify that you are logged in.

• The

button shows your user name and the currently selected button design.

button on the Main screen. You

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On the Main screen, press the

button, and select

My Options

Programmed Operation

Operation

• The

Press the button design that corresponds to the design you want, and press the OK button to accept.

• The Main screen returns to view, and the new button design is displayed until you log out.

Choose Button Type

screen appears.

• The selection is saved and displayed the next time you log in.

Programmed Operation

You can store programs in ultracentrifuge memory. Each program can contain up to five steps (a step contains a set of run parameters). Programs are retained in memory even after powering down the ultracentrifuge.

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Operation

Programmed Operation

Creating a New Program

In the Main screen, press the

• The list of menu options appears.

button.

Select Program.

• The

• If no programs have been created and saved, the screen will be blank.

Select Progra

m screen appears.

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Press the

NEW PROGRAM

button.

Operation

Programmed Operation

• The

Select a rotor first.

• The rotor you select will determine the available speed settings.

a. Press the

• The

Program Summary

ROTOR

Choose Rotor

screen appears.

button.

screen appears.

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Operation

Programmed Operation

Select the desired rotor, and press the OK button to accept.

• The name of the selected rotor displays on the

Press the

• The

NEW STEP

Enter Step Speed

button.

screen appears.

Program Summary

screen.

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• The acceptable values for run speed are shown above the display field: from 5000 to 150,000 RPM, depending on the selected rotor.

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Enter the speed on the keypad display, and press the OK button to accept.

Operation

Programmed Operation

• The

• The acceptable values for run duration are shown above the display field: from 00:01 to

Enter the run time on the keypad display, and press the OK button to accept.

• The

Enter Step Duration

99:59 HH:MM.

Enter Step Temperature

screen appears.

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• If you enter a number of minutes greater than 59, the ultracentrifuge automatically recalculates the time in hours and minutes.

• The acceptable values for run temperature are shown above the display field: from 0to40°C.

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Operation

Programmed Operation

Enter the temperature on the keypad display, and press the

button to accept.

• The

Program Summary

• If you enter a number greater than 40, the

Repeat Steps 6–9 to enter the parameters for up to five steps for a complete run.

• The

Program Summary

screen appears and displays the parameters for the first step.

button will be grayed out.

screen displays the parameters of the steps you’ve entered.

— The Accel value is for the first step in the program, and the Decel value is for the last

step in the program.

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• After you enter five steps, the

NEW STEP

button is grayed out.

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To change the acceleration/deceleration rates, press either the

ACCEL

Operation

Programmed Operation

DECEL

button.

• The

a. To use the maximum values for both rates, go to Step 12.

• Note that the default rates of “

Select the desired rates by touching the corresponding numbers.

• The approximate time duration for the rate you select is displayed on the right.

Select Accel/Decel Rates

— If you select a deceleration rate of coast, “

screen appears.

Max

” (maximum) appear in the

COAST

Time

” appears in the

fields.

Time

field.

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Operation

Programmed Operation

Press the OK button to accept.

• The rates you select display on the

Press the OK button to accept.

• The

Enter Program Name

screen appears.

Program Summary

screen.

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Type the name of the program using the keypad just as you would a keyboard.

• The name of the program appears at the top of the screen.

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Operation

Programmed Operation

a. Note that the

• To enter lowercase letters, press the

b. Press c. To clear the entire entry, press

Press the OK button to accept.

• The name of the program appears in the list of programs.

BACKSPACE

— You now have a new, saved program in memory.

SHIFT

key is green to indicate that uppercase letters are the default.

SHIFT

key.

to erase entered characters ne at a time.

CLEAR

NOTE

Repeat this procedure to add and save additional programs.

Pressing the OK button with a program name selected in this screen loads the program into the

ultracentrifuge.

Starting a Programmed Run

In the Main screen, press the

• The list of menu options appears.

button.

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Operation

Programmed Operation

Select

Program

• The

NOTE

Select the name of the program you want to run, and press the OK button.

Select Program

No Program Selected

selected.

screen appears.

is highlighted in this list, it means that no saved program is currently

• The Main screen returns to view, and the name of the selected program appears in the windshield.

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• The run parameters for the first step appear above the display fields in the Main screen.

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Programmed Operation

Press the

• The Main screen reflects the start of the run.

• This screen updates continuously to reflect the progress of each step in the program.

START

button.

Operation

a. If you are not logged in and

required to log in.

• For more information, see Logging In.

b. If you are starting a run without selecting a rotor and a message appears asking you to

select a rotor, press

• You’ll need to select a rotor.

• For more information, see Selecting a Rotor.

To terminate a run for any reason, press the

• The run will end automatically when the in the program.

— When the rotor comes to a complete stop, a tone will sound.

User Login

in the message box.

has been turned on by the Administrator, you are

STOP

button.

Time

display counts down to zero for the last step

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Operation

Programmed Operation

To exit from program mode:

a. Press the b. In the c. Press

d. Alternatively, you can try changing a parameter such as speed, time, or temperature.

Select Program

• The Main screen returns to view so that you can enter parameters manually.

• A message appears asking you to confirm that you want to exit from program mode.

button in the Main screen.

Making Changes to a Program

You can modify any part of a program: the steps, the Accel/Decel rates, and the rotor.

screen, select

No Program Selected

In the Main screen, press the

• The list of menu options appears.

button.

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Select

Program

Operation

Programmed Operation

• The

a. If necessary, use the arrows to bring additional program names into view.

Select the name of the program you want to modify.

• The name of the program is highlighted, and the

Select Program

screen appears.

EDIT PROGRAM

button becomes available.

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Operation

Programmed Operation

Press the

EDIT PROGRAM

button.

• The

Program Summary

screen displays the steps and other parameters of the selected

program.

You can do any of the following:

a. To delete a step, select the desired step and press the b. To edit a step, selected the desired step and press the

DELETE STEP

EDIT STEP

• For more information, see Creating a New Program.

c. To add a step (if there are fewer than five steps in the program), press the

button.

NEW STEP

button.

• For more information, see Creating a New Program.

d. Change the Accel/Decel rate.

• For more information, see Creating a New Program.

e. Select a different rotor.

• For more information, see Creating a New Program.

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Deleting a Program

In the Main screen, press the

• The list of menu options appears.

button.

Operation

Programmed Operation

Select

Program

• The

Select the name of the program you want to delete.

a. If necessary, use the arrows to bring additional program names into view.

• The name of the program is highlighted, and the

available.

Select Program

screen appears.

DELETE PROGRAM

button becomes

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Operation

Pulse Run Operation

To delete the program, press the

• A confirmation message appears.

Press the

YES

button.

DELETE PROGRAM

button.

• The program is deleted and removed from the list of saved programs.

Pulse Run Operation

Set up the ultracentrifuge as described in Preparing the Ultracentrifuge.

In the Main screen, press the

• The list of menu options appears.

button.

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Select

Pulse

PULSE

• The

To start the run, press and hold down the

button becomes available on the Main screen.

PULSE

button.

Operation

Pulse Run Operation

• The Pulse run starts. — The windshield in the Main screen turns green and reads

a. If you are not logged in and

required to log in.

• For more information, see Logging In.

NOTE The vacuum system will turn on automatically when you press and hold down the

PULSE

button.

User Login

has been turned on by the Administrator, you are

Pulsing

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To stop the run, release the

• The vacuum system will turn off and vent after you release the stops spinning.

• The Main screen redisplays the settings in use prior to Pulse mode operation.

PULSE

button.

PULSE

button and the rotor

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Operation

Remote Operation

The Optima MAX-XP ultracentrifuge can be operated from a remote computer using the software and hardware provided in Remote Control Kit 393395. For information on ordering this kit, contact Beckman Coulter.

Administrator Operations

This section describes the procedures performed by the Administrator. The Administrator performs system level operations, such as setting the date and time, adding and deleting users, turning privileges has access to Administrator functions.

User Login

Accessing Administrator Options

on and off, and turning

Rotor Logging

on and off. Any user with Administrator

NOTE

You must have Administrator privileges to access these features. To find out of you have Administrator

privileges, check with your system administrator.

Verify that you have Administrator privileges.

On the Main screen, press the

• The

Administrator Options

button, and select

screen appears.

Admin.

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You can do the following from this screen:

• Set the audio volume. — See Setting Audio Volume.

• Set the interface language. — See Set Interface Language.

• Set the date and time. — See Setting the Date and Time.

• Add and delete users, and set Administrator privileges. — See Adding and Managing Users.

• Export data. — See Exporting Data.

• Turn on User Login to require users to log in prior to starting a run. — See Setting User Login.

Operation

Administrator Operations

• Turn on Rotor Logging. — See Setting Rotor Logging.

Set Interface Language

You may configure the ultracentrifuge touchscreen for user interaction with different languages. Follow the steps below to configure language settings.

Verify that you have Administrator privileges.

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Operation

Administrator Operations

On the Main screen, press the

button, and select

Admin

• The

Press the

• The

Administrator Options

LANGUAGE

Language Settings

button.

screen appears.

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Press a language from the language options list.

NOTE

Select either the Up or Down arrow keys to view the full list of available languages.

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Press OK.

• A screen appears and prompts you to restart the system.

Pres

YES

• The system restarts and displays the selected language.

Operation

Administrator Operations

Setting the Date and Time

Go to the Administrator Options screen as described in Accessing Administrator Options.

Press the

• The

SET TIME

Set System Date and Time

button.

screen appears.

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Press the large arrows to set the desired date and time.

• The time and date are set.

a. To change the date or time rapidly, hold down the corresponding arrow.

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Operation

Administrator Operations

Press either format.

a. Press the

12 hr

24 hr

button to accept.

to configure the system to display time in either 12- or 24-hour

• Time and date configurations are set, and the

Adding and Managing Users

Adding a User

Go to the

Press the

• The

Administrator Options

MANAGE USERS

Manage Users

screen as described in Accessing Administrator Options.

button.

screen appears.

Administrator Options

screen returns to view.

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Press the

• The

ADD USER

Enter User Name

button.

screen appears.

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Enter a user name on the keypad.

a. When finished, press the

• The

Enter PIN

# screen appears.

button to accept.

Operation

Administrator Operations

Note that the

a. To enter lowercase letters, press the b. Press c. To clear the entire entry, press

Enter a PIN number, and press the OK button to accept.

• The

NOTE

SHIFT

key is green to indicate that uppercase letters are the default.

SHIFT

BACKSPACE

Confirm PIN #

PINs can be from one to six numbers long. The PIN number is masked as you enter it.

to erase entered characters one at a time.

CLEAR

screen appears.

key.

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Operation

Administrator Operations

Enter the PIN number again, and press the

button to accept.

• The

To grant this user Administrator privileges, see Setting Administrator Privileges.

a. To add another user, repeat Steps 2–6.

Manage Users

screen returns to view, and the new user name is added to the list.

Deleting a User

Go to the

Press the

• The

Administrator Options

MANAGE USERS

Manage Users

button.

screen appears.

screen as described in Accessing Administrator Options.

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Select the desired user name in the list, and press the

• A message appears to allow you to confirm your action.

Press the

YES

button to confirm.

DELETE USER

Administrator Operations

button.

Operation

• The

Manage Users

screen returns to view, and the user name is deleted from the list.

Setting Administrator Privileges

In this procedure, you give other users Administrator privileges.

NOTE

You must have Administrator privileges to perform this procedure.

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Operation

Administrator Operations

Go to the Administrator Options screen as described in Accessing Administrator Options.

Press the

MANAGE USER

S button.

• The

Select the desired user name from the list, and press the

• A message appears to allow you to confirm your action.

Manage Users

screen appears.

SET ADMIN

button.

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Press the

• The user with this login name can now perform Administrator operations.

YES

button confirm.

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Setting User Login

You can require users to log in prior to starting a run. This builds a users’ run log for the ultracentrifuge.

Go to the

Press the

Administrator Options

USER LOGIN

button.

Operation

Administrator Operations

screen as described in Accessing Administrator Options.

• The

Press the OK button to accept.

• When a user presses the

This feature is a toggle. To turn off

• The

USER LOGIN

User Login

button turns green to indicate that

button turns gray in the

is now off.

User Login

START

button to start a run, the user will be required to log in.

User Login

, repeat this procedure.

Administrator Options

has been turned on.

screen to indicate that

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3-49

Operation

Administrator Operations

Setting Rotor Logging

When

Rotor Logging

selected, the user will be prompted to select the rotor in use.

Go to the

Press the

Administrator Options

ROTOR LOGGING

is turned on, the user must select a rotor before starting a run. If a rotor is not

screen as described in Accessing Administrator Options.

button.

• The

Press the OK button to accept.

• The ultracentrifuge will keep track of rotor usage.

• Users will be required to select a rotor prior to starting a run (if no rotor is selected).

This feature is a toggle. To turn off

ROTOR LOGGING

button turns green to indicate that

Rotor Logging

, repeat this procedure.

Rotor Logging

has been turned on.

• This toggles the now off.

Populating the Rotor Library

In this procedure, you develop a list of rotors that are available to users at your site.

NOTE

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You must have Administrator privileges to add rotors to and delete rotors from the rotor library.

ROTOR LOGGING

button back to gray to indicate that

Rotor Logging

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On the Main screen, press the

ROTOR

button.

Operation

Administrator Operations

• The

Press the

• The

Choose Rotor

ADD ROTOR

Rotor Catalog

screen appears.

button.

screen appears.

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• The rotor catalog lists all rotors that can be used with the Optima MAX-XP.

a. Select rotor names from the rotor catalog to develop the library of rotors available at

your site.

b. If necessary, use the arrows to bring additional rotors into view.

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Operation

Administrator Operations

Select the desired rotor, and press OK to accept.

• The

Enter the serial number for the selected rotor, and press OK to accept.

• The

Enter Rotor Serial Number

Enter Rotor Run Count

screen appears.

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Enter the rotor run count, and press OK to accept.

Operation

Administrator Operations

• The

NOTE

To add another rotor, repeat Steps 2–5.

• The list of available rotors is available for selection by users of the ultracentrifuge.

Choose Rotor

library.

The rotor run count is the number of runs a rotor has already accumulated.

screen returns to view, and the newly added rotor is listed in the rotor

To delete a rotor from the rotor library, select the rotor entry from the list, and press the

DELETE ROTOR

• A message appears to allow you to confirm your action.

Press the

• The rotor is deleted from the rotor library.

This action does not delete the rotor from the rotor catalog.

BACK

NOTE

Press

button.

YES

button to confirm.

to return to the Main screen.

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3-53

Operation

Administrator Operations

Setting Audio Volume

Go to the

Select the desired audio volume.

• The audio levels are: Mute, Low, Med, High.

Press the OK button to accept.

• The audio level adjusts to the new volume level, and the Main screen returns to view.

Administrator Options

screen as described in Accessing Administrator Options.

Exporting Data

The ultracentrifuge automatically saves data associated with each run. You can export this run data for use in a spreadsheet program such as Microsoft Excel. The data, which consists of the set and actual parameters, user information, and rotor type and serial number, is saved in .csv format. To optimize instrument performance, we recommend exporting the data and then deleting data files from the system periodically.

Go to the

Press the

• You are prompted to insert a memory storage device into the USB slot on the right-hand

Administrator Options

EXPORT DATA

side of the ultracentrifuge.

button.

screen as described in Accessing Administrator Options.

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Operation

Administrator Operations

Insert the memory storage device into the USB port, and press the

• When the data is exported successfully, a confirmation message is displayed.

To delete the data files you just exported, press the

• A message appears stating that all run history will be removed, and it asks you to confirm the operation.

DELETE DATA

EXPORT DATA

button.

Press the

• When the data is deleted, a confirmation message is displayed.

YES

button.

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Operation

Administrator Operations

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Introduction

This section lists possible malfunctions, together with probable causes and corrective actions. Maintenance procedures are described in CHAPTER 5, Troubleshooting.

For any problems not covered here, contact Beckman Coulter Field Service (1-800-742-2345 in the United States; world-wide offices are listed on the outside back cover of this manual) for assistance.

CHAPTER 4 Troubleshooting

NOTE

It is your responsibility to decontaminate the ultracentrifuge, as well as any rotors and/or accessories,

before requesting service by Beckman Coulter Field Service.

User Messages

Messages pop up on the touchscreen interface to communicate information about the ultracentrifuge or to alert you to conditions that require your attention. Dialog boxes with diagnostic messages contain a red border, as shown in the example in Figure 4.1. The possible diagnostic types are:

• CPU

• Power

• Speed

• Vacuum

• Temperature

• Drive

• Imbalance

• Door

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NOTE

The user should wait 10 seconds between turning instrument power off and then turning power

on to clear an error condition.

4-1

Troubleshooting

User Messages

Figure 4.1 Example User Message on Touchscreen Interface

Table 4.1 User Message Chart

Message Definition/Result Possible Cause and Recommended Action

CPU Errors 101 through 113

Microprocessor malfunction or loss of program memory/Deceleration without brake

• If the microprocessor malfunction was caused by power failure, the error can be cleared by turning power off and back on; otherwise, no user action. (In case of program memory loss, the ultracentrifuge is still functional for manual operation.)

• Call Beckman Coulter Field Service.

Power Errors 201 and 202

Loss of power during centrifugation

A power outage has occurred during the run. If power is restored while the rotor is spinning, the run will resume. If the rotor came to a stop, however, restart the run.

Power Error 203 Loss of power during

centrifugation/Deceleratio n without brake

A power outage has occurred during the run. Power was restored, but the run could not continue.

1. Turn the ultracentrifuge power off and back on.

2. If the problem persists, call Beckman Coulter Field

Service.

Power Errors 204 through 207

Power supply error/Deceleration without

No user actions are recommended. Call Beckman Coulter Field Service.

brake

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Speed Error 301 No rotor

installed/Deceleration with brake

1. Verify that the rotor is installed properly.

2. If the problem persists, call Beckman Coulter Field

Service.

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Troubleshooting

Table 4.1 User Message Chart (Continued)

Message Definition/Result Possible Cause and Recommended Action

User Messages

Speed Errors 302 and 303

Speed Error 304 Speed error/Deceleration

Speed error/Deceleration without brake

without brake

NOTE

This condition will result in a 166-minute delay before the diagnostic message can be cleared and the door opened. The power MUST be left on so the ultracentrifuge can calculate when the delay period is completed. If power to the ultracentrifuge is lost or turned off, the remaining delay period is preserved, and the door will remain locked when power is restored until the delay period is completed.

1. Verify that the rotor is installed properly.

2. Verify that the ultracentrifuge is resting on a level

surface.

3. Verify that the set speed is correct for the rotor in use.

4. Verify that the rotor load is within the limits specified in the rotor manual.

5. Verify that the magnets on the bottom of the rotor are undamaged.

6. If the message persists, call Beckman Coulter Field Service.

This message indicates an overspeed condition.

1. Verify that the rotor is installed properly.

2. Verify that the ultracentrifuge is resting on a level

surface.

3. Verify that the magnets on the bottom of the rotor are undamaged.

4. If the message persists, call Beckman Coulter Field Service.

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Speed Error 305 Speed error/Deceleration

with brake

Speed Errors 306 and 307

Speed error/Deceleration with brake

This message indicates a speed signal problem.

1. Verify that the rotor is installed properly.

2. Verify that the ultracentrifuge is resting on a level

surface.

3. Verify that the magnets on the bottom of the rotor are undamaged.

4. If the message persists, call Beckman Coulter Field Service.

No user actions are recommended. Call Beckman Coulter Field Service.

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Troubleshooting

User Messages

Table 4.1 User Message Chart (Continued)

Message Definition/Result Possible Cause and Recommended Action

Speed Error 308 Set speed is above the

maximum speed of the installed rotor/set speed is reset to the rotor maximum and the run continues

Speed Errors 309 and 310

Vacuum Error 401

Speed error/Deceleration with brake

Chamber pressure is above 500 microns/Deceleration with brake

Vacuum Error 402

Chamber pressure is above 31.5 microns for longer than 5 minutes/ If ultracentrifuge is running, it decelerates with brake

No user action is required.

No user actions are recommended. Call Beckman Coulter Field Service.

1. Make sure that the door O-ring is clean, undamaged, and properly lubricated.

2. Check for sample leakage. Clean and dry the rotor chamber if needed.

3. If the problem persists, call Beckman Coulter Field Service.

1. Make sure that the door O-ring is clean, undamaged, and properly lubricated.

2. Check for sample leakage. Clean and dry the rotor chamber if needed.

3. If the problem persists, call Beckman Coulter Field Service.

Vacuum Error 403

Vacuum Error 404

Vacuum Error 405

Vacuum Error 406

Chamber pressure didn’t reach 31.5 microns within 45 minutes/If ultracentrifuge is running, it decelerates with brake

Vacuum level error/Deceleration with brake

Vacuum not venting properly/Deceleration with brake

Vacuum offset error/Deceleration with brake

1. Make sure that the door O-ring is clean, undamaged, and properly lubricated.

2. Check for sample leakage. Clean and dry the rotor chamber if needed.

3. If the problem persists, call Beckman Coulter Field Service.

1. Make sure that the door O-ring is clean, undamaged, and properly lubricated.

2. Check for sample leakage. Clean and dry the rotor chamber if needed.

3. If the problem persists, call Beckman Coulter Field Service.

No user action. Call Beckman Coulter Field Service.

4-4

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Troubleshooting

Table 4.1 User Message Chart (Continued)

Message Definition/Result Possible Cause and Recommended Action

User Messages

Temperature Errors 501 through 504

Drive Error 601 Drive error/Deceleration

Drive Error 602 Drive

Drive Errors 603 through 605

Drive Error 606 Drive error/Deceleration

Excessive or uncontrolled rotor temperature/Deceleration with brake

without brake

overheated/Deceleration without brake

Drive error/Deceleration without brake

without brake

No user action. Call Beckman Coulter Field Service.

NOTE

This condition will result in a 166-minute delay before the door can be opened. The power MUST be left on so the instrument can calculate when the delay period is completed.

No operator action. Call Beckman Coulter Field Service.

NOTE

This condition will result in a 166-minute delay before the door can be opened. The power MUST be left on so the instrument can calculate when the delay period is completed.

1. Check that the rotor is properly installed.

2. Check that the ultracentrifuge is resting on a level

surface.

3. Check that the magnets on the rotor bottom are undamaged.

4. If the message persists, call Beckman Coulter Field Service.

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Drive Error 607 Drive error/Deceleration

without brake

Drive Error 608 Drive error/Deceleration

without brake

1. Press OK to clear the error.

2. If the error message is still displayed, turn

instrument power off and back on.

3. If the problem persists, call Beckman Coulter Field Service.

1. Wait for the rotor to decelerate to 0 rpm.

2. Press OK to clear the error.

3. If the error message is still displayed, turn

instrument power off and back on.

4. If the problem persists, call Beckman Coulter Field Service.

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Troubleshooting

Retrieving Your Sample in Case of Power Failure

Table 4.1 User Message Chart (Continued)

Message Definition/Result Possible Cause and Recommended Action

Drive Error 609 Drive error/Deceleration

without brake

Imbalance Error 701

Door Errors 801 through 803

Rotor imbalance detected/Deceleration with brake

Door open or unlocked/Deceleration with brake

1. Press OK to clear the error.

2. If the error message is still displayed, turn

instrument power off and back on.

3. If the problem persists, call Beckman Coulter Field Service.

1. Verify that the rotor is secured to the drive hub.

2. Check for proper balance and placement of tubes

and/or buckets.

3. Restart the run.

4. If the problem persists, call Beckman Coulter Field

Service.

1. Close the door properly and restart the run.

2. If the problem persists, call Beckman Coulter Field

Service.

Retrieving Your Sample in Case of Power Failure

If facility power fails only momentarily, the ultracentrifuge will resume operation (where it left off) when power is restored and the rotor will return to set speed. However, if the rotor came to a complete stop, you will need to restart the run when the power is restored. In either case, the

POWER

occurred.

message will appear on the touchscreen interface to indicate that a power outage has

4-6

WARNING

Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and/or mechanical injury. Therefore, turn the power OFF and disconnect the ultracentrifuge from the main power source, and refer such maintenance to service personnel.

In the event of an extended power failure, it may be necessary to trip the door-locking mechanism manually to remove the rotor and retrieve your sample.

WARNING

The following procedure should be implemented only when absolutely necessary and only by qualified service personnel.

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Troubleshooting

Retrieving Your Sample in Case of Power Failure

Turn the power off and disconnect the power cord from the main power source.

The underside of the top cover is fitted with tabs that snap inside clips in the side panels (see

Figure 4.2). By inserting the blade of a flat-head screwdriver into the release slot on either side

of the ultracentrifuge, you can pop off the top cover of the ultracentrifuge.

CAUTION

LISTEN CAREFULLY! Do not proceed if any sound or vibration is coming from the drive.

Figure 4.2 Removing the Top Cover of the Ultracentrifuge

393552AH

1. Release slot (located on right and left sides 2. Clips for securing cover

Remove the top cover and set it aside.

Locate the vacuum solenoid valve (see Figure 4.3), and turn the screw clockwise until you hear air rush into the chamber.

• The chamber vacuum is released.

a. If you hear a whining noise, the rotor is still spinning. Close the valve and wait until the

noise stops.

• When the sound of rushing air stops, the vacuum is vented.

4-7

Troubleshooting

Retrieving Your Sample in Case of Power Failure

Turn the screw counterclockwise to its closed position.

Locate the door lock system (see Figure 4.3), and push the spring-loaded pin down to unlock the door.

a. If the rotor is still spinning, close the door and wait.

• The drive is very quiet and may emit no audible sounds below 10,000 RPM.

WARNING

NEVER try to slow or stop the rotor by hand.

Figure 4.3 Door Lock System

4-8

1. Screw

2. Spring Loaded Pin

Open the door and remove your sample.

3. Door Lock System

4. Vacuum Solenoid

393552AH

Beckman Coulter Optima MAX-XP Instructions For Use Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Revision History

Alerts for Danger, Warning, Caution, and Note

Safety Notice

Safety During Installation and/or Maintenance

Electrical Safety

Safety Against Risk of Fire

Mechanical Safety

Chemical and Biological Safety

Summary of Instrument Labels

Contents

Illustrations

Tables

Certification

Introduction

Scope of Manual

Typographic Conventions

CFC-Free Centrifugation

Radio Interference

Description

CHAPTER 1

Description

Temperature Sensing and Control

Drive

Controls and Indicators

Safety Features

Name Rating Plate

Specifications

Available Rotors

Introduction

CHAPTER 2

Preinstallation Requirements

Space Requirements

Electrical Requirements

Introduction

CHAPTER 3

Operation

Touchscreen Interface

Summary of Optima MAX-XP Run Procedures

Manual Operation

Programmed Operation

Pulse Run Operation

Remote Operation

Administrator Operations

Introduction

CHAPTER 4

Troubleshooting

User Messages

Retrieving Your Sample in Case of Power Failure