Beckman Coulter Microfuge 20 Instructions For Use Manual

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Page 1

Instructions For Use

Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.

Microfuge 20 Series

Centrifuges

B30162AA May 2013

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Microfuge 20/20R

Beckman Coulter Ireland, Inc. Mervue Business Park, Mervue Galway, Ireland 353 91 774068

Centrifuges

PN B30162AA (May 2013)

No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without prior written permission from Beckman Coulter, Inc.

Beckman Coulter and the stylized logo are trademarks of Beckman Coulter, Inc. and are registered in the USPTO.

All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

Find us on the World Wide Web at:

www.beckmancoulter.com

Made in Germany Printed in the U.S.A.

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Initial Issue, 05/2013

Microfuge 20 Series Centrifuges Instructions for Use, version B30162AA

Revision Status

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Revision Status

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Safety Notice

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate the instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.

Alerts for Danger, Warning, Caution, Important, and Note

DANGER

DANGER indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

IMPORTANT

Following the advice in the Important adds benefit to the performance of a piece of equipment or to a process.

NOTE NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

IMPORTANT is used for comments that add value to the step or procedure being performed.

Safety During Installation and/or Maintenance

The centrifuge weighs 13 kg/28.6 lb (nonrefrigerated model) without rotor, or 32 kg/70.5 lb (refrigerated model) without rotor. DO NOT attempt to lift or move one of them without assistance from another person.

Any servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury. Make sure that the power switch is turned off and the instrument is disconnected from the main power source by removing the Mains (power) plug from the outlet receptacle, and refer such servicing to qualified personnel.

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Do not replace any centrifuge components with parts not specified for use on this instrument.

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Safety Notice

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect this equipment to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the instrument.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet

receptacle strip.

• Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into the instrument and damage electrical or mechanical components.

• Work on the power supply system must be performed by certified electricians.

• Inspect the electrical equipment of the unit regularly. Defects such as loose or burnt cables

must be eliminated immediately.

Safety Against Risk of Fire

This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol) in this centrifuge nor handle or store them within the 30 cm (12 inches) clearance envelope surrounding the centrifuge. Do not use the centrifuge within hazardous locations.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the rotors and accessories designed for use in this centrifuge.

• Before starting the centrifuge, make sure that the rotor tie-down screw is securely fastened.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop the rotor by hand.

• NEVER attempt to override the door interlock system while the rotor is spinning.

• Maintain a 30 cm (12 inches) clearance envelope around the centrifuge while it is running.

During operation you should come within the envelope only to adjust instrument controls, if necessary.

• Never bring any flammable substances within the 30 cm (12 inches) area surrounding the centrifuge.

• Never operate the instrument without a rotor installed.

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• If the instrument housing becomes damaged do not use the instrument. Contact Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at http://www.beckmancoulter.com).

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Such materials should not be used in this instrument unless all necessary safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to their use.

• Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

Safety Notice

Chemical and Biological Safety

• Dispose of all waste solutions according to appropriate environmental health and safety guidelines.

• Do not centrifuge materials that could result in a hazardous chemical reaction.

It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter.

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Safety Notice

Chemical and Biological Safety

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Revision Status,iii

Safety Notice,v

Introduction,xv

CHAPTER 1: Description,1-1

Introduction,1-1

Centrifuge Function and Safety Features,1-1

Centrifuge Function,1-1 Models,1-2 Safety Features,1-3

Contents

Chassis,1-3

Housing,1-3 Door,1-3 Rotor Chamber,1-4 Drive,1-4

Temperature Sensing and Control (refrigerated models only),1-4

Controls and Indicators,1-5

Power Switch,1-5 Control Panel,1-5

System Keys,1-5 Program Keys,1-6 Digital Displays,1-6

Name Rating Plate,1-7

Microfuge 20 Nonrefrigerated Model Specifications,1-8

Microfuge 20R Refrigerated Model Specifications,1-9

Available Rotors,1-10

CHAPTER 2: Operation,2-1

Introduction,2-1

Run Procedure,2-2

Preparation and Loading,2-2

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Contents

Entering Run Parameters,2-4

Menu Key Sequence,2-4 Setting Run Speed,2-5 To set the speed,2-5 Setting Run Time,2-5 To set a timed run,2-6 To start a continuous run,2-6 To stop a continuous run,2-7 Setting Run Temperature (Microfuge 20R refrigerated models

only),2-7 To set the run temperature,2-7 Rotor Code Selection,2-8 To select the correct rotor,2-8 Precooling the centrifuge (20R refrigerated models only),2-8 To precool the centrifuge,2-8 Setting Acceleration and Deceleration Rates,2-9 To activate both Soft-start and Soft-stop functions ,2-9 To activate only the Soft-stop function ,2-10 To deactivate both Soft-start and Soft-stop functions ,2-10

Starting a Run,2-10

To start a run,2-10

Short Run (Quick Run) Function,2-11

To perform a quick run on the non-refrigerated Microfuge

20,2-11 To perform a quick run on the refrigerated Microfuge 20R,2-12

Changing Parameters During a Run,2-12

To change the speed during a run,2-12 To change the run time during a run,2-13 To change the temperature during a run (Microfuge 20R

only),2-13

Stopping a Run,2-13

To stop a run in progress,2-13

Unloading,2-14

Summary of Run Procedures,2-14

Saving and Using Programs,2-16

Saving Settings,2-16

To save settings into a program,2-16 To run a saved program,2-17 To change a saved program,2-17

CHAPTER 3: Troubleshooting,3-1

Introduction,3-1

User Messages,3-1

To clear the problem or error,3-2

Certification

Beckman Coulter Microfuge 20 series centrifuges are manufactured in a facility that maintains certifications to ISO 9001:2008 or ISO 13485:2003. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the the safety requirements for electrical equipment for laboratory use of applicable regulatory agencies. Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com.

Introduction

Scope of Manual

This manual is designed to familiarize you with the Beckman Coulter Microfuge 20 series centrifuges, their functions, specifications, operation, and routine operator care and maintenance. Beckman Coulter recommends that you read this entire manual, especially the Safety Notice section and all safety-related information, before operating the centrifuge or performing instrument maintenance.

The following introductory pages contain the instrument specifications, as well as space, electrical, and temperature conditions required for optimal centrifuge performance. A list of available rotors is also included.

• CHAPTER 1, Description provides a brief physical and functional description of the centrifuge,

the operating controls and indicators, and system specifications.

• CHAPTER 2, Operation contains centrifuge operating procedures.

• CHAPTER 3, Troubleshooting lists diagnostic messages and other possible malfunctions,

together with probable causes and suggested corrective actions.

• CHAPTER 4, Care and Maintenance contains procedures for routine operator care and

maintenance, as well as a brief list of supplies and replacement parts.

• APPENDIX A, Installation contains instructions for installing and connecting the centrifuge.

• APPENDIX B, Table of Hazardous Substance’s Name and Concentration contains information

required by Electronic Industry Standards of the People’s Republic of China.

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NOTE

If the centrifuge is used in a manner other than specified in this manual, the safety and performance of this equipment could be impaired. Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.

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Introduction

Conventions

Certain symbols are used in the product labeling to call out safety-related and other important information. These international symbols may also be displayed on the centrifuge and are reproduced on the inside of the back cover of this manual.

Typographic Conventions

Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as keys and displays.

• Key names (for example, appear in bold type.

• Cursor keys, used to increment values up or down when setting parameters, are shown as up and down arrows ( or ).

CFC-Free Centrifugation

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Microfuge 20 series centrifuges.

Symbols and Labels

This section provides information for some labels and symbols appearing on the Microfuge 20 instrument housing. These labels and symbols may be associated with user-serviceable procedures. Individual hazards associated with a specific procedure in this manual may use these labels and symbols, and are included in Warnings or Cautions within the procedures for that task.

OPEN or START) and display names (for example, TEMP°C or SPEED)

Biohazard

xvi

This caution symbol indicates biohazardous risk from possible patient specimen contamination.

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Caution Symbol

This symbol indicates a caution message and appears adjacent to an explanation or other symbols that define the caution.

High Voltage Danger

Introduction

Symbols and Labels

This symbol indicates high voltage is present or that there is a risk of electric shock when working in this area. Operation, replacement or servicing of any components where contact with bare, live hazardous parts could occur, possibly resulting in electric shock, should only be performed by your Beckman Coulter representative.

Protective Ground

This symbol is used to indicate a protective ground. This instrument must be properly grounded. Do not under any circumstances operate the instrument unless it is properly grounded.

Recycling Label

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A28219-AA

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE) Directive of the European Union. The presence of this marking on the product indicates:

• the device was put on the European Market after August 13, 2005 and

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Introduction

Symbols and Labels

• the device is not to be disposed of via the municipal waste collection system of any member state of the European Union.

It is very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing this label, please contact your dealer or local Beckman Coulter office for details on the take-back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.

Risk of Fire Warning

Before replacing fuses, shut off power and disconnect the power cord. Failure to do so can cause electric shock and/or equipment damage. Replace fuses only with approved type and rating replacement fuse.

Electric Shock Warning

Operation, replacement or servicing of any components where contact with electronic components could occur can result in electric shock, and should only be performed by your Beckman Coulter representative.

Rotor Rotation

This label indicates the direction of the rotor rotation.

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China RoHS Caution Label

This label and materials declaration table (the Table of Hazardous Substance’s Name and

Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006

“Marking for Control of Pollution Caused by Electronic Information Products” requirements.

This logo indicates that this electronic information product contains certain toxic or hazarous elements, and can be used safely during its environmental protection use period. The number in the middle of the logo indicates the environmental protection use period for the product. The outer circle indicates that the product can be recycled. The logo also signifies that the product should be recycled immediately after its environmental protection use period has expired. The date on the label indicates the date of manufacture.

Introduction

Symbols and Labels

Australian EMC Compliance Label

The C-Tick mark is intended for use on products that comply with Australian Communication Authority (ACA) EMC Requirements.

TUV-GS Label

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The GS seal indicates that a product has been inspected in a national testing center and that production facilities have undergone an initial, as well as on ongoing, inspection. Only companies that meet these high standards may attach the GS seal next to the CE seal or CE label.

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Introduction

Symbols and Labels

CE Label

This label indicates conformance to various directives set forth under European Union law.

Consult IFU Label

This label indicates that the Instructions for Use should be referred to for more information.

RoHS Conform Label

This label indicates that the product is compliant with the EU RoHS directive (2002/95/EC) pertaining to the Restriction of Hazardous Substances in Electrical and Electronic Equipment (EEE).

TUV-NRTL Label

This label indicates recognition by a Nationally Recognized Test Laboratory (NRTL) that the instrument has met the relevant product safety standards.

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Introduction

This chapter provides a brief physical and functional description of Beckman Coulter Microfuge 20 series centrifuges. The operating controls and indicators are also described; instructions for their use are in

CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in

Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.

Centrifuge Function and Safety Features

CHAPTER 1 Description

Centrifuge Function

Figure 1.1 Microfuge 20 (A) and Microfuge 20R (B) Front View

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1. Door

2. Display

3. Emergency Door Release

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Description

Centrifuge Function and Safety Features

Figure 1.2 Microfuge 20R (A) and Microfuge 20 (B) Rear view

Models

1. Power switch

2. Power input

Beckman Coulter Microfuge 20 series bench top centrifuges generate centrifugal forces required for a wide variety of applications. Together with the Beckman Coulter rotors designed specifically for use in this centrifuge, the instrument applications include:

• Nucleic acid preps and protein preps using common kits and columns.

• Routine processing such as sample preparations, pelleting, extractions, purifications,

concentrations, phase separations and receptor binding.

• Rapid sedimentation of protein precipitates, particulates, and cell debris.

The centrifuges are microprocessor-controlled, providing interactive operation. The instrument design features a brushless three-phase drive system, and a choice of acceleration/deceleration rates. The refrigerated models also have temperature control systems.

Microfuge 20 series centrifuges are available in both nonrefrigerated and refrigerated models. See Microfuge 20 Nonrefrigerated Model Specifications or Microfuge 20R Refrigerated Model

Specifications for the operating differences between the two models. Unless indicated otherwise,

information in this manual is the same for both models.

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Safety Features

Microfuge 20 series centrifuges have been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft).

Instrument safety features include:

• An electromechanical door-locking mechanism to prevent operator contact with spinning rotors. When the door is closed it locks automatically. It can be unlocked only by pressing the

OPEN key, and opened only when the power is on and the rotor is at rest.

• The centrifuge can only be started when the door is properly closed. The electrical lock must be locked. The door can only be opened when the rotor has stopped. If the door is opened by way of the emergency release system during operation, the centrifuge will immediately switch off and decelerate brakeless. If the door is open, the drive is completely separated from the mains power supply, that is, the centrifuge cannot be started.

• A steel barrier surrounds the rotor chamber to provide full operator protection.

• An overspeed system continuously monitors the rotor during centrifugation. The system

includes a magnetic sensor on the drive motor. Throughout the run, checks are made to ensure that the rotor does not exceed set speed.

• The centrifuge feet, made of rubber, have been designed to minimize possible rotation in the event of a rotor mishap.

• An internal system continually monitors the sensor signals to confirm that they are within the expected values. Malfunctions are indicated by error messages with a number in the speed/RCF display. See Troubleshooting.

Description

Chassis

Housing

Door

• For the Microfuge 20R, if the temperature inside the rotor chamber rises above 50 °C, the drive system will be switched off automatically. The centrifuge cannot be restarted until it has cooled.

The centrifuge housing is made of high-performance engineered plastic, finished with urethane paint. The control panel is covered by a protective overlay made of coated polycarbonate.

The door is made of a solid sheet of steel, encased in foam molding. In the center of the door is a window for strobe viewing. An electromechanical door lock system prevents a run initiation unless the door is shut and latched. The door is locked when a run is in progress and can be opened only when the rotor is stopped. In the event of a power failure, the door lock can be manually released for sample recovery (see CHAPTER 3, Troubleshooting).

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Description

Temperature Sensing and Control (refrigerated models only)

Rotor Chamber

The rotor chamber is shown in Figure 1.3. The drive shaft and rubber boot surrounding the drive shaft are visible in the chamber bottom. A gasket system around the chamber opening ensures sealing. (Instrument gaskets have not been designed as bioseals for aerosol containment.)

Figure 1.3 Interior View of the Rotor Chamber

1. Drive Shaft

2. Boot

3. Gasket

Drive

The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation. A tiedown screw is used to attach the rotor to the drive shaft. The resilient suspension ensures that loads are not disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs during centrifugation. Maximum braking may be selected to reduce deceleration time, allowing fast processing of samples; alternately, delicate gradients may be preserved using slower deceleration.

Temperature Sensing and Control (refrigerated models only)

With the power on, the temperature control system is activated when the door is closed. The run temperature can be set between –10 and +40°C in refrigerated models. If no set temperature is entered, the centrifuge automatically selects the last entered temperature. (For the first run of a new centrifuge, the instrument selects 20°C as its operating temperature.) A thermistor in the rotor chamber continuously monitors chamber temperature. The micro-processor calculates the required chamber temperature to maintain the selected rotor temperature.

1-4

NOTE

In the unlikely event of a complete cooling system failure, the drive switches off if the chamber

temperature goes above 50°C. Restarting the centrifuge is not possible until the chamber is cooled.

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Controls and Indicators

Power Switch

The power switch is located on the centrifuge back panel (see Figure 1.2). This two-position rocker switch (

I, on; O, off) controls electrical power to the centrifuge.

Description

Controls and Indicators

NOTE

Control Panel

The control panel is mounted at an angle on the front of the centrifuge and includes system keys, programming keys, and digital displays (see Figure 1.4).

Figure 1.4 Microfuge 20 and Microfuge 20R Control Panels

The power must be turned on before the chamber door can be opened.

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System Keys

The centrifuge operation is controlled through the system keys.

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Description

Controls and Indicators

START/STOP Pressing the START/STOP key causes a centrifuge run to begin. This key can also be

OPEN Pressing the OPEN key unlatches the centrifuge door lock and allows the door to be

QUICK RUN Manually pressing this key initiates a short run for as long as the key is pressed (applies

Program Keys

The program keys are used to set run parameters (a program consists of all of the parameters for a run). Except for the cursor keys, program keys show the parameters as they are input.

used to abort a deceleration process and restart the centrifuge.

The START/STOP key can be pressed to end a run. The centrifuge decelerates to a complete stop according to the preselected deceleration curve. Deceleration can be terminated and the centrifuge restarted by pressing START again.

opened. The centrifuge accepts this command only when the rotor is completely stopped.

only to Microfuge 20).

 

(cursor keys)

PROG Use this key to recall a previously saved program or to enter program mode to save

PRECOOL

(refrigerated

model only)

MENU Use this key to scroll through and highlight the different run parameters. The

The cursor keys are up and down arrow keys, which can be pressed to increment values up or down when setting parameters.

run parameters as a program.

Use this key to cool the rotor at a slow speed before a run starts. See Precooling the

centrifuge (20R refrigerated models only)for instructions on using this function.

MENU key is pressed until the appropriate time, speed, or temperature display function flashes. The parameter is set with the cursor keys.

Digital Displays

Digital displays indicate rotor speed, run time, rotor chamber temperature, and the selected acceleration and deceleration profiles (see Figure 1.5). When the power is turned on, they show the operating parameters of the most recent run performed before the power was turned off. The displays serve a dual purpose.

1-6

• When the run parameters are being set (the input mode), the displays show the set values (those selected by the operator). When a run- parameter key (for example,

MENU) is pressed,

the appropriate display flashes to indicate that data can be entered.

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Description

Name Rating Plate

• The actual (real-time) operating conditions of the centrifuge are displayed during the run, after

START is pressed.

NOTE The Error Message Chart also appear on the displays, when applicable. The centrifuge emits a series

of audible tones to alert the user to an error condition.

Figure 1.5 Digital Display showing all possible displays, Microfuge 20R shown

Table 1.1 Display Definitions

Display Description

Soft-Stop/Start Off Acceleration and Deceleration settings.

Short Run Displays while the Quick Run button (Microfuge 20 only) is pressed or when the

Start button is pressed and held (Microfuge 20R only).

Prog Displays to show the saved program number.

Set Displayed when the speed, run time or temperature is being set.

Actual Displayed to show the actual speed, run time and temperature.

Speed/RCF Displays the speed in either RPMs or RCF (´ g)

Time Displays the time to be set for the run or the actual time while running.

Temperature Displays the temperature to be set for the run or the actual temperature while

running (Microfuge 20R only)

Rotor code Code 1: Not used

Code 2: FA241.5 Code 3: FA241.5P Code 4: FA4x8.2P Code 5: FA361.5 Code 6: Not used

Name Rating Plate

The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge. Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge.

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Description

Microfuge 20 Nonrefrigerated Model Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee.

Speed

Set speed

Speed control

Speed display

Time

Set time

Time display

Acceleration Fast, soft See Acceleration and Deceleration Rates by Rotor (in

Deceleration Fast, soft See Acceleration and Deceleration Rates by Rotor (in

to 15,000 rpm (in 100-rpm increments) min: 200 rpm actual rotor speed, ±50 rpm of set speed actual rotor speed in 100-rpm increments or in RCF (when selected)

10 sec to 99 min, 59 sec in one second increments or continuous (¥) time remaining in run (timed run ±1 minute) or ¥ and elapsed time (continuous run)

minutes:seconds) for times

minutes:seconds) for times.

Programs 10

Ambient temperature range 5 to 40°C

Humidity restrictions <80% (noncondensing)

Maximum altitude 2000 m (6562 ft.)

Dimensions

Width

Depth

Height, door closed

Height, door open

Weight 13 kg (28.6 lb)

Clearances (sides) 30 cm (11.8 in.)

Electrical requirements

120-V Instrument 100-V instrument

220–240-V instrument

Electrical supply Class I

Maximum heat dissipation into room

under steady-state conditions

Noise level 0.91 m (3 ft) in front of

instrument (approx.)

31 cm (12.2 in.) 42 cm (16.5 in.) 27 cm (10.7 in.) 53 cm (20.7 in.)

120 VAC, 2.3 A, 60 Hz 100 VAC, 2.9 A, 50/60 Hz 220–240 VAC, 1.4 A, 50 Hz

580 Btu/h (170 W)

< 59 dB

Installation (overvoltage) category II

Pollution degree

a. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected.

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Microfuge 20R Refrigerated Model Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee.

Speed

Set speed Speed control Speed display

Time

Set time

Time display

Temperature

Set temperature

Temperature control (after equilibration)

Temperature display (after equilibration)

Operating range

Acceleration Fast, soft See Acceleration and Deceleration Rates by Rotor (in

Deceleration Fast, soft See Acceleration and Deceleration Rates by Rotor (in

Programs 10

Ambient temperature range 5 to 35°C

Humidity restrictions <80% (noncondensing)

Maximum altitude 2000 m (6562 ft.)

Dimensions

Width

Depth

Height, door closed

Height, door open

Weight 32 kg (70.5 lb)

Clearances (sides) 30 cm (11.8 in.)

Electrical requirements

120-V instrument 100-V instrument

220–240-V instrument

Electrical supply Class I

Maximum heat dissipation into room under

steady-state conditions

Noise level 0.91 m (3 ft) in front of

instrument (approx.)

Installation (overvoltage) category II

Pollution degree

a. Temperature range depends on rotor in use and speed (see applicable rotor manual).

b. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected.

to 15,000 rpm (in 100-rpm increments) min: 200 rpm actual rotor speed, ±50 rpm of set speed actual rotor speed in 100-rpm increments or in RCF (when selected)

to 99 min, 59 sec in one second increments or continuous (¥) time remaining in run (timed run ±1 min accuracy) or ¥ and elapsed time (continuous run)

–10 to +40°C (in 1°C increments) ±2.5°C of set temperature chamber temperature in 1°C increments

–10 to 40°C

minutes:seconds) for times.

31.0 cm (12.2 in.)

66.0 cm (26.0 in.)

29.1 cm (11.5 in.)

54.7 cm (32.0 in.)

120 VAC, 5.3 A, 60 Hz 100 VAC, 6.4 A, 50/60 Hz 220–240 VAC, 2.4 A, 50 Hz

1433 BTU/h (420 W)

< 58 dB

Description

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Description

Available Rotors

See the applicable rotor manual for information on rotor use, care and maintenance, and rotor accessories.

Rotor Profile

FA241.5

Fixed Angle, 44° Angle

FA241.5P

Fixed Angle, 43° Angle

FA4´8.2P

Fixed Angle, 44° Angle

Description

= 82.0 mm

max

Rotor code: 2

= 82.0 mm

max

Rotor code: 3

= 66.0 mm

max

Tube 1 & 8 at r Tube 2 & 7 at r Tube 3 & 6 at r Tube 4 & 5 at r

Rotor code: 4

= 66.0 mm

max

= 63.0 mm

max

= 60.0 mm

max

= 59.0 mm

max

Max RCF

(´ g)

Max

RPM

Max

Capacity

(mL)

Rotor Part

Number

Rotor

Manual

Number

20,627 15,000 24 ´ 2.0 B30155 B30178

20,627 15,000 24 ´ 2.0 B30156 B30178

15,000 32 ´ 200 mL B31059 B33057

16,602

15,847

15,093

14,841

FA361.5

Fixed Angle

Inner row: 50° Angle

Outer row: 30° Angle

Inner row:

= 80 mm

max

Outer row:

= 80 mm

max

20,124 15,000 36 ´ 2.0 B31057 B33026

Rotor code: 5

a. Refer to the individual manuals for each rotor for more detailed specifications.

b. Rotor is certified for biocontainment by a third-party test facility CAMR, Porton Down, UK, or USARMIID, Ft. Detrick, MD, U.S.A.). Improper use or

maintenance may affect seal integrity and thus containment.

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Introduction

This section contains operating procedures for the centrifuge, using the rotor designed for use in these centrifuges. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation. To prevent condensation, keep the centrifuge door closed and the power turned off ( is not in use.

CHAPTER 2 Operation

O) when the centrifuge

NOTE

If the centrifuge is used in a manner other than that specified in this manual, the safety and

performance of this equipment could be impaired.

WARNING

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Operator error or tube failure may generate aerosols. Do not run potentially hazardous materials in this centrifuge unless all appropriate safety precautions are taken. Always use the appropriate rotors and adapters.

Handle all infectious samples according to good laboratory practices and methods to prevent the spread of disease. Ask your laboratory safety officer to advise you about the level of containment required for your application and about the proper decontamination or sterilization procedures to follow if fluids escape from containers. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. Because spills may generate aerosols, observe proper safety precautions for aerosol containment.

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Operation

Run Procedure

WARNING

Risk of personal injury or property damage. The centrifuge must not be used in the vicinity of flammable liquids or vapors, and such materials should not be run in the centrifuge. During operation you should come within the 30 cm (12 in.) clearance envelope only to adjust the instrument controls, if necessary. Never bring any flammable substances within the 30 cm (12 in.) area surrounding the centrifuge. Do not lean on the centrifuge or place items on the centrifuge while it is operating.

Run Procedure

The following detailed operating procedures are summarized at the end of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps.

Preparation and Loading

NOTE Before installing the rotor, lubricate it following the instructions in the rotor manual.

Check the name rating plate for the correct voltage, then plug the power cord into the wall receptacle.

Press the power switch to on (I).

Press the OPEN key and lift the door up; it remains in the open position.

Use the T-handle wrench to turn the rotor tie-down screw to the left (counterclockwise).

a. Remove the tie-down screw.

Remove the rotor.

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Operation

Run Procedure

Make sure that the tapered sleeve is in place at the base of the centrifuge drive shaft before installing the rotor (see Figure 2.1) and wipe the sleeve to be sure that it is clean and dry.

• The rotor rests on the sleeve while spinning, and does not operate properly if the sleeve is missing.

Figure 2.1 Tapered Sleeve Side View

NOTE If the tapered sleeve comes off, it must be replaced by a Beckman Coulter Field Service

representative. Call Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com).

CAUTION

Do not drop the rotor onto the drive shaft. The shaft can be damaged if the rotor is forced sideways or dropped onto it. Install the rotor by centering it over the shaft and carefully lowering it straight down.

Install the rotor according to the instructions in the rotor manual.

CAUTION

Always run the rotor with a balanced load.

Close the centrifuge door and push firmly down on both sides of the door front until the clicking (latching) sound stops.

Remove the rotor from the centrifuge if a long period between runs is anticipated.

CAUTION

If the rotor is left in the centrifuge between runs, make sure the rotor is seated on the drive shaft and the tie-down screw is tight before each run.

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Select the correct rotor code. See Rotor Code Selection.

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Operation

Run Procedure

Entering Run Parameters

When the power is applied for the initial use (no previous runs), default values are displayed (see Figure 2.2). After the initial use, the parameters of the latest previous run are displayed when power is applied.

When run parameters for a rotor have been entered, as described below, they are retained in the centrifuge memory. The recalled program can then be used for the current run or altered as required. See Saving Settings.

Figure 2.2 Default Parameters on First-Time Use, Microfuge 20R shown

1. 15,000 rpm

2. 2 min duration

3. temperature: 20°C

4. no soft acceleration

Menu Key Sequence

Pressing the MENU key starts the parameter change sequence. Repeatedly pressing the MENU key steps through parameter sequence. When the parameter units (rpm, rcf, m & s, soft-off, soft stop/start on, soft stop on) flash, press  or  to change that parameter. The entire parameter change sequence follows these steps:

1. Speed a. in RPM b. in RCF

2. Time in minutes and seconds

3. Temperature (for Microfuge 20R only)

4. Acceleration/Deceleration Options a. Soft off b. Soft-stop/start on c. Soft-stop on

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Operation

Run Procedure

Setting Run Speed

The RCF value is determined by the rotor geometry and speed. The RCF and speed values, therefore, depend on each other. If one of the two values is entered, the other value will be set automatically.

Centrifuge speed can be set for the maximum rated speed of the selected rotor. Either revolutions per minute (RPM) or relative centrifugal field (RCF) can be used to select speed. During centrifugation, the

SPEED display indicates the actual run speed (RPM) of the rotor.

To set the speed

Press the MENU key until the speed unit flashes on the display.

• The RPM unit on the (in 100-RPM increments) with the cursor keys.

• To enter the speed in RCF, press the

• Program mode ends if no other keys are pressed within 15 seconds.

Press the  or  arrow key until the required RPM or RCF speed is displayed.

• The corresponding RCF is automatically calculated by the centrifuge, but the RPM value is displayed during the run.

• You can check the RCF during the run by pressing the  or  key while the centrifuge is running.

Press the MENU key until all screen functions stop flashing, to confirm the selection.

• The speed setting will be saved after approximately 20 seconds if no other key is pressed within this time period.

SPEED display flashes, indicating that the RPM can be entered

MENU key again. The speed in RCF flashes.

Setting Run Time

The run time is displayed in the middle line of the display. During centrifugation, the remaining run time is displayed. The run time of the centrifuge can be set at one-second intervals up to 99 minutes and 59 seconds.

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Run time can be set for either a timed run or continuous operation.

Timed Run

Time can be set for up to 99 minutes and 59 seconds (if the seconds parameter entered exceeds 59, it is automatically converted into minutes). During centrifugation, the down when the rotor starts to spin and continues the count-down until deceleration begins. The

TIME display shows the time remaining in the run, in minutes and seconds. When the time

display reaches zero, the run ends.

TIME display begins counting

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Operation

Run Procedure

Continuous Run

During a continuous run, the run time of the centrifuge is unlimited and must be stopped manually. The centrifuge accelerates during the continuous run until the set speed is reached. During centrifugation the TIME display begins counting up, when the rotor starts to spin.

To set a timed run

Press the MENU key until the time unit flashes on the display.

Program mode ends if no other keys are pressed within 15 seconds.

Press the  or  cursor key until the required run duration is displayed. Pressing and holding either key causes the parameter to change more rapidly.

Press the MENU key until all screen functions stop flashing, to confirm the selection.

The time setting will be saved after approximately 15 seconds if no other key is pressed within this time period.

To start a continuous run

Press the MENU key until the time unit flashes on the display.

Program mode ends if no other keys are pressed within 15 seconds.

Press the  cursor key until the display switches from 00:10 to --:--¥.

After 99 minutes and 59 seconds, any additional run time will no longer be displayed, but the centrifugation will continue.

Press the MENU key until all screen functions stop flashing, to confirm the selection.

The time setting will be saved after approximately 15 seconds if no other key is pressed within this time period.

Close the centrifuge door and push firmly down on both sides of the door front until the clicking (latching) sound stops.

Press the START/STOP key. The continuous run will start.

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During centrifugation the TIME display begins counting up when the rotor starts to spin.

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Operation

Run Procedure

To stop a continuous run

Press the START/STOP key.

Deceleration begins immediately. The elapsed time shows during deceleration. The run ends.

Setting Run Temperature (Microfuge 20R refrigerated models only)

Run temperature can be set between –10 and +40°C. The typical operating range is from +2°C to 40°C, depending on the rotor and speed selected.

The temperature in the rotor chamber is displayed in the lower third of the centrifuge display. The display alternates between the set temperature and the actual temperature. The actual temperature is marked by the word “actual” in front of the temperature value.

NOTE

NOTE Temperatures may vary slightly between instruments. If sample temperature is crucial, test

To set the run temperature

The actual temperature flashes at 10 second intervals. When changing the temperature parameter,

be careful not to confuse the flashing of the actual temperature with the menu unit that also flashes.

temperature settings on your instrument using water samples.

Press the MENU key until the temperature unit flashes on the display.

TEMP°C display flashes, indicating that the temperature can be entered with the

The cursor keys.

Program mode ends if no other keys are pressed within 15 seconds.

Press the  or  cursor key until the required run temperature is displayed.

NOTE

Press the MENU key until all screen functions stop flashing, to confirm the selection.

The temperature setting will also be accepted and saved after approximately 15 seconds if no other key is pressed within this time period.

For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast

equilibration.

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NOTE

Opening the door turns refrigeration off. Closing the door restarts refrigeration.

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Operation

Run Procedure

Rotor Code Selection

Select the installed rotor to allow for the correct RCF value to be selected and displayed.

NOTE

To select the correct rotor

All of the available rotors for the Microfuge 20 and Microfuge 20R have the same rated speed

of 15,000 RPMs.

Press and hold the MENU key until the rotor unit flashes on the display.

Press the  or  cursor key until the correct rotor is displayed.

Rotor codes

• Code 1: Not used

• Code 2: FA241.5

• Code 3: FA241.5P

• Code 4: FA4x8.2P

• Code 5: FA361.5

• Code 6: Not used

Press the MENU key to confirm the selection.

The rotor code setting will be saved after approximately 15 seconds if no other key is pressed within this time period.

Install the rotor. See the specific rotor manual for complete information.

Precooling the centrifuge (20R refrigerated models only)

To maintain sample integrity during low temperature runs it is recommended to precool the empty rotor and instrument prior to the run. This centrifuge has a special program that precools the centrifuge under defined conditions.

NOTE

To precool the centrifuge

Once the precooling program is loaded, the set temperature can only be set to values below the actual temperature. If the limits of the setting range are reached during the setting process, the temperature display flashes for approximately one second.

Press the PRECOOL key to load the precooling program. Precool on displays.

The display shows 1/3 of the maximum rotor speed and the corresponding RCF value. The runtime field indicates ‘

¥’ (continuous run). The set temperature cannot be higher than the

actual temperature.

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Run Procedure

Press the START/STOP key.

Once the rotor reaches 1/3 of the maximum rotor speed (5000 RPM) and the precool temperature value is reached and remains stable for one minute, the program ends with the indication Precool OK.

Operation

NOTE

If the precooling program is selected and then deselected without actually being started, the set temperature will be reset to the old value. If, however, the precooling run is started, the limited set temperature will also be used for the following runs, but it will not be saved in the program.

Setting Acceleration and Deceleration Rates

Microfuge 20 series centrifuges feature two acceleration rates — fast and soft (or slow), and two deceleration rates — fast and soft (or slow). See below for actual times of fast and soft acceleration and deceleration by rotor.

Table 2.1 Acceleration and Deceleration Rates by Rotor (in minutes:seconds)

Microfuge 20 Microfuge 20R

Rotor

Acceleration Deceleration Acceleration Deceleration

Fast Soft Fast Soft Fast Soft Fast Soft

FA241.5 0.26 1:03 0:22 1:05 0:26 1:02 0:22 1:05

FA241.5P 0:16 1:03 0:16 1:05 0:16 1:02 0:16 1:05

FA4x8.2P 0:11 1:03 0:13 1:05 0:11 1:02 0:13 1:05

FA361.5 0:26 1:03 0:22 1:05 0:26 1:02 0:22 1:05

a. Times are approximate; actual times vary depending on the the rotor load, run speed, and

voltage fluctuations.

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To activate both Soft-start and Soft-stop functions

Press the MENU key until the Soft-stop/start function flashes on the display.

Program mode ends if no other keys are pressed within 15 seconds.

Press the  or  cursor key until the Soft-stop/start on is displayed.

Both soft-start and soft-stop functions are now activated.

Press the MENU key to confirm the selection.

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Operation

Run Procedure

To activate only the Soft-stop function

Press the MENU key until the Soft-stop/start function flashes on the display.

Program mode ends if no other keys are pressed within 15 seconds.

Press the  or  cursor key until the Soft stop on is displayed.

The soft-stop function is now activated.

Press the MENU key to confirm the selection.

To deactivate both Soft-start and Soft-stop functions

Press the MENU key until the Soft-stop/start function flashes on the display.

Program mode ends if no other keys are pressed within 15 seconds.

Press the  or  cursor key until the Soft off is displayed.

Both soft functions are now deactivated.

Press the MENU key to confirm the selection.

Starting a Run

The run can be started using the parameters in memory from a previous run, or using new or changed parameters that you enter using the procedures described above.

To start a run

Check that all parameters are correct and the door is closed and latched.

Press the START/STOP key.

• An actual set speed greater than the rotor’s maximum permitted speed, results in an error code and the centrifuge shuts down.

— See CHAPTER 3, Troubleshooting for information on error codes.

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Operation

Run Procedure

— Throughout the run, checks are made to ensure that the rotor does not exceed

set speed.

• The

SPEED display indicates the actual rotor speed in RPMs.

— The RCF can be displayed by pressing he  or  cursor key.

— This display also shows the time remaining in the run (or ¥ and elapsed time for

continuous operation).

TEMP display indicates the actual temperature of the rotor.

WARNING

Do not attempt to override the door interlock system while the rotor is spinning.

CAUTION

Do not lift or move the centrifuge while the rotor is spinning. Do not place items on the centrifuge during operation.

Short Run (Quick Run) Function

During a short run, the centrifuge accelerates at a maximum rate until the set speed is reached. The short run function, accessed by pressing the as follows:

QUICK RUN key, is used for short-duration runs

NOTE

• When the QUICK RUN key is pressed, the rotor accelerates at a maximum rate to the set speed

• When the

• The centrifuge memory retains the parameters of the last run performed before the

To perform a quick run on the non-refrigerated Microfuge 20

The Microfuge 20R Refrigerated centrifuge does not have a dedicated QUICK RUN button,

but pressing and holding the START/STOP button serves the same function.

and continues to spin as long as long as the acceleration, and deceleration settings are overridden by the short run function.) When the

QUICK RUN key is released, the rotor begins decelerating to 0 RPM using maximum deceleration.

QUICK RUN key is pressed, the TIME display begins displaying the elapsed seconds.

When the

key was pressed. At the end of a quick run, after the centrifuge door is opened and closed the previous run parameters are displayed.

Close the centrifuge door and push firmly down on both sides of the door front until the clicking (latching) sound stops.

For the Microfuge 20, press and hold the QUICK RUN key.

The message

QUICK RUN key is released, the seconds stop accumulating.

Short Run and the duration of the quick run is displayed.

QUICK RUN key is pressed. (The current run time,

QUICK RUN

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Operation

Run Procedure

Release the QUICK RUN key.

• When the a standstill.

• After the quick run and the centrifuge has come to a complete stop, the door unlocks automatically and the program that was set beforehand is displayed again.

To perform a quick run on the refrigerated Microfuge 20R

Close the centrifuge door and push firmly down on both sides of the door front until the clicking (latching) sound stops.

For the Microfuge 20R, press and hold the START/STOP key.

The message

Release the START/STOP key.

• When the standstill.

• After the quick run and the centrifuge has come to a complete stop, the door unlocks automatically and the program that was set beforehand is displayed again.

QUICK RUN key is released, the centrifuge decelerates at maximum rate to

Short Run and the duration of the quick run is displayed.

START/STOP key is released, the centrifuge decelerates at maximum rate to a

Changing Parameters During a Run

While a run is in progress, speed, time and temperature (Microfuge 20R only) run parameters can be altered without stopping the run. Run duration can also be changed from continuous to a specified time period, or from a specified time period to continuous.

NOTE

To change the speed during a run

The deceleration rate cannot be changed after deceleration starts.

Press the MENU key until the desired speed function flashes on the display.

• The last digit on the lowered with the cursor keys.

Press the  or  cursor key until the required RCF or RPM is displayed.

The new parameter take effect immediately.

SPEED display flashes, indicating that the RPM or RCF can be raised or

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To change the run time during a run

Press the MENU key until the time function flashes on the display.

Operation

Run Procedure

• The last digit on the with the cursor keys.

Press the  or  cursor key until the required run time is displayed.

The new parameter takes effect immediately.

NOTE

To change the temperature during a run (Microfuge 20R only)

Press the MENU key until the temperature function flashes on the display.

• The last digit on the

Press the  or  cursor key until the required temperature is displayed.

The new parameter takes effect immediately.

If the run time is changed during the run, the centrifuge will run for the entire new time and will

disregard the previous runtime that has already elapsed.

raised or lowered with the cursor keys.

TIME display flashes, indicating that the time can be raised or lowered

TEMPERATURE display flashes, indicating that the temperature can be

Stopping a Run

A timed run ends automatically when the TIME display counts down to zero.

To stop a run in progress

Press the START/STOP key for normal deceleration or the deceleration selected for the run.

After the rotor stops spinning, press the OPEN key to release the door latches, then open the door.

NOTE

For Microfuge 20R refrigerated centrifuges: To prevent chamber icing, use a sponge to wipe

condensation out of the chamber bowl between runs.

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Operation

Unloading

NOTE When you remove the rotor, make sure that the tapered sleeve from the centrifuge drive shaft

does not come out with the rotor. If the tapered sleeve is inside the rotor drive hole, call Beckman Coulter Field Service at 1-800-742-2345 (U.S.A or Canada); outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com).

After completing a run, unload the rotor following the instructions in the applicable rotor manual.

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

Summary of Run Procedures

For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibrium. For low-temperature runs, precool the centrifuge by running a 30-minute cycle (with a precooled rotor installed) at the required temperature with the speed set at 2000 rpm.

Press the POWER switch to on (I).

a. Open the centrifuge door (press the

Make sure that the tapered sleeve is in place at the base of the centrifuge drive shaft before installing the rotor.

• The rotor does not operate properly if the sleeve is missing.

Install the rotor according to the instructions in the applicable rotor manual.

OPEN key and lift the door up).

CAUTION

Always run the rotor with a balanced load.

Close the centrifuge door and push firmly down on both sides of the door front until the clicking (latching) sound stops.

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Summary of Run Procedures

Enter run parameters:

Set run speed — MENU until RPM or RCF displays, use  or  until correct speed displays.

•

Set run duration — MENU until time displays, use  or  until correct time displays.

•

Set run temperature (Microfuge 20R only) — MENU until temperature displays, use  or 

until correct temperature displays.

•

Set acceleration/deceleration — MENU until the Soft-stop/start on, soft-stop on, or soft off

displays, use  or  until correct deceleration displays.

Check that all parameters are correct and the door is shut and latched, then press the

START/STOP key.

WARNING

Never attempt to override the door interlock system while the rotor is spinning.

Operation

CAUTION

Do not lift or move the centrifuge while the rotor is spinning.

Wait for the set time to count down to zero, or end the run by pressing or holding the

START/STOP key.

After the rotor stops spinning, press the OPEN key to release the door latch; open the door.

Unload the rotor according to instructions in the applicable rotor manual.

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures applied to the centrifuge and accessories.

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Operation

Saving and Using Programs

The program is used to save or load certain recurring settings of the centrifuge. Ten separate programs can be saved and reused.

NOTE

to cool the centrifuge without vessels. See Precooling the centrifuge (20R refrigerated models only).

Saving Settings

The following parameters that are set to be run can be saved as part of a reusable program:

• Speed

• Run time

• Temperature (Microfuge 20R only)

• Acceleration

• Deceleration

• Rotor type/code

To save settings into a program

Press the PROG key.

Press the  or  cursor key to scroll and select an unused program number.

The display

The precooling program does not occupy any storage location and cannot be deleted. It is used only

Prog ## flashes (# represents numbers 01 through 10).

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NOTE Scrolling through the programs causes the parameters of each program to display.

NOTE Program numbers that are already occupied will be overwritten with the current data.

NOTE The programs that are not in use display the default settings. See Entering Run Parameters for

the default settings.

Using the MENU key and the  or  cursor keys, change speed, time, temperature, and/or acceleration parameters. See Entering Run Parameters for details.

Press the MENU key to confirm the selections and the program. The program is saved.

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Saving and Using Programs

To run a saved program

Press the PROG key.

Press the  or  cursor key to scroll and select the program number to be run.

Press PROG until Prog ## stops flashing.

Close the centrifuge door and push firmly down on both sides of the door front until the clicking (latching) sound stops.

Press the START/STOP key.

Operation

To change a saved program

Press the PROG key.

Press the  or  cursor key to scroll and select the desired program number to be changed.

Using the MENU key and the  or  cursor keys, change speed, time, temperature, and/or acceleration parameters.

Press the MENU key to confirm the selections and the program. The program is changed.

Close the centrifuge door and push firmly down on both sides of the door front until the clicking (latching) sound stops.

Press the START/STOP key.

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Operation

Saving and Using Programs

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Introduction

This section lists possible malfunctions, together with probable causes and corrective actions required. Maintenance procedures are contained in CHAPTER 4, Care and Maintenance. For any problems not covered here contact Beckman Coulter Field Service.

CHAPTER 3 Troubleshooting

NOTE

It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories,

before requesting service by Beckman Coulter.

User Messages

If a problem occurs during operation, the rotor decelerates to a stop, an error code appears on the

SPEED display. Such problems may result from incorrect input or from an equipment malfunction.

Refer to Table 3.1 to determine the cause of the problem and recommended actions. If you are unable to correct the problem, call Beckman Coulter Field Service. To help diagnose and correct the problem, gather as much information about the situation as you can:

• Write down the error number that appears on the display.

• Note the operating situation when the error occurred (rotor in use, speed, load type, etc.).

• Note any unusual environmental and/or operating conditions (ambient temperature,

voltage fluctuations, etc.).

• Add any other information that may be helpful.

Malfunctions are indicated by error messages with a number in the speed/RCF display. See Figure 3.1 for an example.

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Troubleshooting

User Messages

In the event of a fatal error (for example, a defective door lock), a certain safety time will be counted down on the display. During this time, is up,

Figure 3.1 Error message example

ERR and SAFE flash alternately on the display. When the time

OFF will be displayed.

CAUTION

Do not switch the centrifuge off until the word ‘OFF’ is displayed! This is necessary to ensure that the rotor is at a complete standstill.

To clear the problem or error

Eliminate the source of the problem (see Table 3.1 and Table 3.2 below).

Press the door key to acknowledge and clear the error message.

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Troubleshooting

Table 3.1 Error Message Chart

Error Number Problem Recommended Action Explanation

User Messages

1 through 9, System error After rotor comes to a complete

stop, turn the power off (O), then back on (I) to reset.

10 through 19, Speedometer error After rotor comes to a complete

stop, turn the power off (O), then back on (I) to reset.

20 through 29 Motor error Turn the power off (O). Ensure

ventilation.

30 through 39 EEPROM error After rotor comes to a complete

stop, turn the power off (O), then back on (I) to reset.

40 through 45 Temperature error

(applies only to Microfuge 20R refrigerated centrifuges)

• Allow to slow down

• Power off

• Allow to cool down

• Provide better ventilation

All of these errors stop the centrifuge or cause it to decelerate brakeless.

Errors 34, 35 and 36 will cause the centrifuge to stop; Errors 37 and 38 will not cause the centrifuge to stop, these errors will only return error messages.

46 through 49 Imbalance error • Allow to slow down

• Power off

• Allow to cool down

50 through 59 Door error • Press door key

• Close door

• Remove foreign matter

from the opening of the door lock device

60 through 69 Process error • Allow to slow down

• Cycle power

70 through 79 Communication error • Allow to slow down

• Cycle power

Errors 50 and 51 will cause the centrifuge to stop.

“With error 60 message “power failure during run”, with error 61, the message “stop after power on”

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Troubleshooting

User Messages

Table 3.1 Error Message Chart (Continued)

Error Number Problem Recommended Action Explanation

80 through 89 Parameter error • Press door key

• Close door

• Remove foreign matter

from the opening of the door lock device

90 through 99 Other errors • Check connections

• Provide sufficient water

throughput (only water cooled centrifuges)

NOTE Contact Beckman Coulter Field Service if error messages persist.

With error 83, error message only.

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Accessing the Centrifuge in Case of Power Failure

If the facility power fails only momentarily, the centrifuge resumes operation when power is restored and the rotor returns to set speed. However, if the rotor comes to a complete stop the centrifuge run must be restarted when the power is restored. In the event of an extended power failure, you may have to trip the door-locking mechanism manually to remove the rotor and retrieve your sample.

WARNING

Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and/or mechanical injury. Therefore, turn the power off and disconnect the instrument from the main power source by removing the Mains (power) plug from the outlet receptacle and refer such maintenance to qualified service personnel.

To gain emergency access the centrifuge

Turn the power switch to off (O) and disconnect the power cord from the main power source by removing its power plug from the receptacle.

WARNING

Never attempt to override the door interlock system while the rotor is spinning.

DANGER

Never try to slow or stop the rotor by hand.

Make sure that the rotor is not spinning.

CAUTION

Do not proceed if there is any sound or vibration coming from the drive.

Remove the plug from the front panel. See Figure 3.2.

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Accessing the Centrifuge in Case of Power Failure

Figure 3.2 Removing the Emergency Access Plug

1. Emergency access plug and position of the opening for the

emergency door release

Troubleshooting

Insert the supplied 4 mm (P/N B33985) Allen wrench horizontally into the hole and turn it counterclockwise until it stops. You should hear the door lock release. See Figure 3.3.

NOTE

Figure 3.3 Manually releasing the door lock with the Allen wrench

If the door is opened via the emergency door release system during a centrifuge run, the

centrifuge will be switched off immediately and decelerate with no brake.

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1. Manual release of the door lock

Reinsert the emergency access plug.

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Troubleshooting

Accessing the Centrifuge in Case of Power Failure

3-8

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Introduction

For maintenance not covered in this manual, contact Beckman Coulter Field Service. User messages are discussed in CHAPTER 3, Troubleshooting. Refer to the applicable rotor manual and to Chemical Resistances (publication IN-175) for instructions on the care of rotors and their accessories.

CHAPTER 4 Care and Maintenance

NOTE

It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before

requesting service by Beckman Coulter Field Service.

WARNING

Any maintenance procedure or servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury. Make sure that the power switch is off (O) and the centrifuge is disconnected from the main power source by removing the Mains (power) plug from the outlet receptacle and refer such servicing to qualified service personnel.

Do not use alcohol or other flammable substances in or near operating centrifuges.

WARNING

Risk of personal injury or contamination. If you do not properly shield yourself while performing service, maintenance, and troubleshooting procedures, residual fluids in the instrument could injure or contaminate you. Beckman Coulter recommends barrier protection, such as appropriate safety glasses, lab coat, and gloves, be worn throughout the performance of service, maintenance, and troubleshooting procedures to avoid contact with cleaners and/or residual fluids in the instrument.

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The centrifuge, rotor and accessories are subject to high mechanical stresses. Thorough and diligent maintenance performed by the user extends the service life of the centrifuge and prevents premature failure.

NOTE

If corrosion or other damage occurs due to improper care, the manufacturer cannot be held liable or

subject to any warranty claims.See the Beckman Coulter, Inc. Microfuge 20 Series Centrifuge Warranty.

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Care and Maintenance

Maintenance

Preventive Maintenance

The following procedures should be performed regularly to ensure continued performance and long service life of the centrifuge.

Regularly inspect the interior of the rotor chamber for accumulations of sample, dust, or glass particles from broken sample tubes.

a. Clean as required (see Cleaning, below), as these accumulations can result in rotor

vibrations.

Regularly check the air intake and exhaust vents for obstructions.

a. Keep vents clear and clean.

Cleaning

To prevent the rotor from sticking, lubricate the drive shaft with Spinkote at least once a month, and after each cleaning.

Frequent cleaning is recommended to prolong the life of the centrifuge. Always clean up spills when they occur to prevent corrosives or contaminants from drying on component surfaces.

NOTE

Before using any cleaning or decontamination methods, except those recommended by the manufacturer, users should check with the manufacturer that the proposed methods will not damage the equipment.

To prevent accumulations of sample, dust, and/or glass particles from broken sample tubes, keep the interior of the rotor chamber clean and dry by frequent wiping with a cloth or

paper towel.

Clean the drive shaft, shaft cavity, threads, and the tie-down screw at least once a week using a mild detergent such as Solution 555 and a soft brush.

a. Dilute the detergent with water (10 parts water to 1 part detergent). b. Rinse thoroughly and dry completely.

4-2

c. Lubricate the drive shaft with Spinkote after cleaning.

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Care and Maintenance

Cleaning

Wash the bowl using a mild detergent such as diluted Solution 555.

a. Rinse thoroughly and dry completely. b. If a cleaning solution other than Solution 555 is used, consult Chemical Resistances

(publication IN-175) or contact the cleaning-solution vendor to verify that the solution will not damage the centrifuge.

Clean the centrifuge case and door by wiping with a cloth dampened with diluted Solution 555. Dilute the detergent with water (10 parts water to 1 part detergent). Do not use acetone or other

solvents.

 If the centrifuge has been contaminated with toxic, radioactive or pathogenic substances, clean

the rotor chamber immediately with a suitable decontamination agent (depending on the type of contamination).

WARNING

 Immediately rinse off the rotor, buckets or accessories under running water if they have come

into contact with any liquids that may cause corrosion. Use a brush for test tubes to clean the bores of angle rotors. Turn the rotor upside down and allow it to dry completely.

 Clean the accessories outside the centrifuge once per week or preferably after each use.

Adapters should be removed, cleaned and dried.

 If the rotors or accessories have been contaminated with toxic, radioactive or pathogenic

substances, clean them immediately with a suitable decontamination agent (depending on the type of contamination). Take suitable precautions for your own safety if there is a risk of toxic, radioactive or pathogenic contamination.

 Dry accessories with a soft cloth or in a drying chamber at approximately 50°C.  The chemical resistance of plastic decreases with rising temperatures. If solvents, acids or

alkaline solutions have been used, clean the plastic accessories thoroughly.

 Aluminum parts are especially susceptible to corrosion. Avoid the use of cleaning agents that

contain acid or that are alkaline on aluminum parts.

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 Microfuge 20R refrigerated units only: Vacuum the vents once every six months.

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Care and Maintenance

Decontamination

If the centrifuge and/or accessories are contaminated with radioactive or pathogenic solutions, perform appropriate decontamination procedures. Refer to Chemical Resistances (IN-175) to be sure the decontamination method will not damage any part of the centrifuge.

Sterilization and Disinfection

The centrifuge is finished with urethane paint. Ethanol (70%) may be used on this surface. See Chemical Resistances for more information regarding chemical resistance of centrifuge and accessory materials.

CAUTION

Ethanol is a flammability hazard. Do no use it in or near operating centrifuges.

While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

Storage and Transport

Storage

Before storing a centrifuge for an extended period, return it to its original shipping container to protect it from dust and dirt. Reinsert the shipping foam (removed at time of centrifuge installation) into the chamber, making sure the drive shaft is stabilized in the hole in the foam. Temperature and humidity conditions for storage should meet the environmental requirements described in the specifications given in CHAPTER 1, Description.

The centrifuge can be stored in its original packaging for up to one year.

• Store the centrifuge only in dry rooms.

• The storage temperature must be above –20°C.

• If the centrifuge needs to be stored for more than one year or needs to be shipped overseas,

contact Beckman Coulter Customer Service.

4-4

Notes on Transport

• When lifting the centrifuge always reach under the centrifuge from the side.

• Use suitable packaging and, if possible, use the original packaging.

• Retain the packaging for possible future transport of the centrifuge.

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Returning a Centrifuge

Before returning a centrifuge or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the authorization form and for packaging and shipping instructions.

To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity. Sterilization and decontamination must be done before returning the parts.

All parts must be accompanied by a signed note, plainly visible on the outside of the box, stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach

this notification will result in return or disposal of the items without review of the reported problem.

Supply List

Contact Beckman Customer Service or visit www.beckmancoulter.com for information about ordering parts, supplies, and publications. For your convenience, a partial list is given below.

Care and Maintenance

Supply List

Refer to the applicable rotor manual for materials and supplies needed for rotors.

Replacement Parts

Description Part Number

Rotor tie-down screw 365806

T-handle wrench 365636

Allen wrench B33985

Other

NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.

Description Part Number

Spinkote lubricant (2 oz) 306812

Silicone vacuum grease (1 oz) 335148

Solution 555 (1 qt) 339555

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Care and Maintenance

Supply List

4-6

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Page 63

Introduction

This chapter contains instructions for installing and connecting the centrifuge. Check that required clearances and electrical power are available.

APPENDIX A

Installation

WARNING

Risk of personal injury. Each centrifuge weighs 13 kg/29 lb (nonrefrigerated model) or 32 kg/71 lb (refrigerated model.) DO NOT attempt to lift or move one of them without assistance from a lifting device or another person.

Installing the Instrument

WARNING

Risk of fire. Do not place the centrifuge near areas containing flammable reagents or combustible fluids. Vapors from these materials could enter the centrifuge air system and be ignited by the motor.

WARNING

Maintain a 30 cm (12 in) clearance envelope around the centrifuge while it is running. No persons should be within this clearance boundary while the centrifuge is operating. Do not handle or store hazardous materials within the 30 cm (12 in) area surrounding the centrifuge.

Considerations to make before installation

• Operate the centrifuge only in closed and dry rooms.

• All of the energy supplied to the centrifuge is converted into heat and emitted to the

ambient air.

• Ensure sufficient ventilation.

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• Allow at least a 30 cm envelope around all sides of the centrifuge so that the vents in the centrifuge remain fully effective.

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Installation

Installing the Instrument

• Do not subject the centrifuge to thermal stress. For example, do not position the centrifuge near a source of heat.

• Do not position the centrifuge where it receives direct sunlight (UV radiation).

• The table or bench that the centrifuge is installed on must be stable and have a solid, flat

surface.

• After moving the centrifuge from cold to warmer rooms, condensation may collect inside the centrifuge. It’s important to allow at least 24 hours for drying before using the centrifuge again.

To install the centrifuge

The centrifuge ships in a cardboard box. For easy access, remove the top of the box, the foam insert on top of the centrifuge, and then the upper part (sides) of the box and set them aside.

a. Then, with the help of another person, move the centrifuge to its final position.

Position the centrifuge on a level surface, such as a sturdy table or laboratory bench that is able to support the weight of the centrifuge and resist vibration.

• Refer to Microfuge 20 Nonrefrigerated Model Specifications or Microfuge 20R Refrigerated Model

• (Note the warning above regarding centrifuge weight.)

Specifications in CHAPTER 1 for weight.

a. Make sure that the centrifuge front feet are fully supported on the table. b. Locate the centrifuge in an area with sufficient ventilation to allow for heat dissipation. c. Check that there are 30 cm (12 in.) clearances at the sides of the centrifuge to ensure

sufficient air circulation. Dimensions are shown in Figure A.1 or Figure A.2. The centrifuge must have adequate air ventilation to ensure compliance to local

requirements for vapors produced during operation. For ambient temperature ranges see Microfuge 20R Refrigerated Model Specifications and

Microfuge 20 Nonrefrigerated Model Specifications.

NOTE

During transport between areas with varying temperatures, condensation may occur inside

the centrifuge. Allow sufficient drying time before running the centrifuge.

A-2

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Electrical Requirements

See the Microfuge 20 Nonrefrigerated Model Specifications or Microfuge 20R Refrigerated Model

Specifications in CHAPTER 1 for centrifuge electrical requirements.

Make sure the voltage and frequency imprinted on the name rating plate affixed to the back of the centrifuge agree with the line voltage and frequency of the outlet used. Refrigeration does not function properly if the frequency [Hz] does not match the name rating plate. Plug in both ends of the centrifuge power cord. If there is any question about voltage, have a qualified service person measure it under load while the drive is operating.

A 1.8-m (6-ft) power cord with grounded plug is supplied with the centrifuge. Make sure that the matching wall outlet is located near the centrifuge and is easily accessible.

Installation

Electrical Requirements

NOTE

Figure A.1 Dimensions of the Non-Refrigerated Centrifuge

The power plug serves as the Disconnecting Device and must remain easily accessible.

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Installation

Electrical Requirements

Figure A.2 Dimensions of the Refrigerated Centrifuge

WARNING

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name rating plate affixed to the centrifuge.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet receptacle strip.

A-4

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Test Run

Installation

Test Run

NOTE The centrifuge must be plugged in and the power switch turned to the on position (I) before the door

can be opened.

Beckman Coulter recommends that you make a test run to ensure that the centrifuge is in proper operating condition following shipment. See CHAPTER 2, Operation for instructions on operating the centrifuge.

After completing the test run, log on to www.beckmancoulter.com to register the centrifuge. This validates the centrifuge warranty and ensures that you receive any further information regarding new accessories and/or modifications as they become available.

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Installation

Test Run

A-6

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APPENDIX B

Table of Hazardous Substance’s

Name and Concentration

This materials declaration table (the Table of Hazardous Susbtance’s Name and Concentration) and the China RoHS Caution Label are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements.

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B-2

有毒有害物质名称及含量的标识格式

Table of Hazardous Substance’s Name and Concentration

电子信息产品号码 EIP Part Number:

产品名称 Product Name: Microfuge 20 Series, Benchtop Centrifuge 产品型号Product Model Number:

B31602 B31606 B31610 B31614

部件名称

Component Name

印刷电路板组件 Printed Circuit Board Sub Assemblies

电缆组件 Cable Sub Assemblies

塑料部件 Plastic Parts

钣金部件 Sheet Metal

连接部件（螺丝，螺栓等） Hardware (screws, bolts, etc.)

电源组件 Power Supply Sub Assembly

Microfuge 20 220V-240V/50 Hz Microfuge 20 IVD 220V-240V/50 Hz Microfuge 20R 220V-240V/50 Hz Microfuge 20R IVD 220V-240V/50 Hz

有毒有害物质或元素

Hazardous Substances Name

铅

汞

镉

OOOO O O

O: 表示该有毒有害物质在该部件所有均质材料中的含量均在 SJ/T 11363-2006 标准规定的限量要求以下

六价铬

Cr6+

多溴联苯

PBBB

多溴二苯醚

PBDE

X: 表示该有毒有害物质至少在该部件的某一均质材料中的含量超出 SJ/T 11363-2006 标准规定的限量要求

（企业可在此处，根据实际情况对上表中打“X”的技术原因进行进一步说明）

O: Indicates that the toxic or hazardous substances contained in all of the homogeneous materials for this part is below the limit requirements in SJ/T 11363-2006.

X: Indicates that the toxic or hazardous substances contained in at least one of the homogeneous materials used in this part is

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above the limit requirements in SJ/T 11363-2006.

(Enterprise may further provide in this box technical explanation for marking “X” based on their actual conditions).

Page 71

Beckman Coulter, Inc.

Microfuge 20 Series Centrifuge Warranty

Subject to the exceptions and upon the conditions specified below, Beckman Coulter agrees to correct, either by repair, or, at its election, by replacement, any defects of material or workmanship which develop within two (2) years after delivery of the Microfuge 20 Series centrifuge (the product), to the original Buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use.

Some components and accessories by their nature are not intended to and will not function for as long as one (1) year. A complete list of such components or accessories is maintained at the factory and at each Beckman Coulter District Sales Office. The lists applicable to the products sold hereunder shall be deemed to be part of this warranty. If any such component or accessory fails to give reasonable service for a reasonable period of time, Beckman Coulter will repair or, at its election, replace such component or accessory. What constitutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter.

Replacement

Any product claimed to be defective must, if requested by Beckman Coulter, be returned to the factory, transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.

Conditions

Beckman Coulter shall be released from all obligations under all warranties either expressed or implied, if the product(s) covered hereby are repaired or modified by persons other than its own authorized service personnel, unless such repair in the sole opinion of Beckman Coulter is minor, or unless such modification is merely the installation of a new Beckman Coulter plug-in component for such product(s).

Disclaimer

IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT NEITHER BECKMAN COULTER, INC. NOR ITS SUPPLIERS SHALL HAVE ANY LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.

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Warranty-1

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Beckman Coulter, Inc. Microfuge 20 Series Centrifuge Warranty

Warranty-2

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English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어

Symbol Symbol Simbolo Symbole Simbolo

Simbole символ

符号

記号

상징

Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목

Dangerous voltage Gefährliche electrische Spannung Voltaje peligroso Courant haute tension Pericolo: alta tensione

Caution, consult accompanying documents Vorsicht, konsultieren Begleitdokumente Atención, consulta documentos adjuntos Attention, consultent des documents d'accompagnement Attenzione, consulta i documenti di accompagnamento

Biohazard Potentiell infektiösem Material Riesgo biológico Risque biologiqu Pericolo biologico

On (power) Ein (Netzverbindung) Encendido Marche (mise sous tension) Acceso (sotto tensione)

Off (power) Aus (Netzverbindung) Apagado Arrêt (mise sous tension) Spento (fuori tensione)

Protective earth (ground) Schutzleiteranschluß Puesta a tierra de protección Liaison à la terre Collegamento di protezione a terra

Earth (ground) Erde (Masse) La tierra (suelo) Terre (sol) Scarica a terra

Manufacturer Hersteller Fab ri ca nte Fab ri ca nt Fabbricante

Authorized representative in the European Community Autorisierter Repräsentant in der Europäischen Gemeinschaft Representante autorizado en la Comunidad Europea Représentant autorisé dans le Communauté européen Rappresentante autorizzato nella Comunità Europea

Consult Instructions for Use Konsultieren Sie Anwendungsvorschriften Consulte las instrucciones para el uso Consultez les instructions pour l'usage Consulti le istruzioni per uso

Tensão perigosa

Опасное напряжение тока

危险电压危険な電圧 위험한 전압

Cuidado, ulta documentos adjuntos Внимание, советует с сопроводительными документами

注意, 咨询附属单证注意, 伴う文書に相談しなさい 주의, 동반 문서를 상담하십시오

Material infeccioso potencial биологической опасности

可能的传染性物潜在的な感染性物質 전염하는 물자

Fora (o poder) На (мощности)

开（电源）ン（電源） 에 (힘)

Fora de (poder)

С (сила)

（电源）

ン（電源） 떨어져 (힘)

Terra de proteção (terra) Защитное заземление (земля)

保护接地保護アース（接地） 방어적인 지구 (지상)

Te r r a Земли

接地アース（接地） 지구 (지상)

Fab ri ca nte производитель

制造商メーカー 제조자

Representante autorizado na Comunidade Européia Утверженный представитель в сообществ

在欧共体的授权代表欧州共同体の承認された代表 유럽 공동체에 있는 허가한 대표자

Consulte instruções para o uso Советуйте с инструкциями для пользы

咨询使用说明书使用説明に相談しなさい 사용 설명을 상담하십시오

Page 74

www.beckmancoulter.com

Beckman Coulter Microfuge 20 Instructions For Use Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Revision Status

Safety Notice

Alerts for Danger, Warning, Caution, Important, and Note

Safety During Installation and/or Maintenance

Electrical Safety

Safety Against Risk of Fire

Mechanical Safety

Chemical and Biological Safety

Certification

Introduction

Scope of Manual

Conventions

CFC-Free Centrifugation

Symbols and Labels

Introduction

Centrifuge Function and Safety Features

CHAPTER 1

Description

Chassis

Temperature Sensing and Control (refrigerated models only)

Controls and Indicators

Name Rating Plate

Microfuge 20 Nonrefrigerated Model Specifications

Microfuge 20R Refrigerated Model Specifications

Available Rotors

Introduction

CHAPTER 2

Operation

Run Procedure

Unloading

Summary of Run Procedures

Saving and Using Programs

Introduction

CHAPTER 3

Troubleshooting

User Messages

Other Possible Problems

Accessing the Centrifuge in Case of Power Failure

Introduction

CHAPTER 4

Care and Maintenance

Maintenance

Cleaning

Decontamination

Sterilization and Disinfection

Storage and Transport

Supply List

Introduction

APPENDIX A

Installation

Installing the Instrument

Electrical Requirements

Test Run

APPENDIX B