Beckman Coulter JS-5.3 Instructions For Use Manual

Instructions For Use

Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

JS-5.3 Swinging-Bucket Rotor

For Use in Beckman Coulter
Avanti J-26S XP Series,
Avanti J-26 XP Series,
Avanti JXN-26,
and Avanti J-E Centrifuges

PN J-TB-089AG
March 2014

JS-5.3 Swinging-Bucket Rotor

PN J-TB-089AG (March 2014)

Copyright ©2001–2014 Beckman Coulter, Inc.

All rights reserved. No part of this document may be
reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.

Avanti, Beckman Coulter, and the stylized logo are
trademarks of Beckman Coulter, Inc. and are registered in
the USPTO.

All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.

Find us on the World Wide Web at:

www.beckmancoulter.com

Printed in the U.S.A.

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to use this equipment. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.

Alerts for Warning, Caution, Important, and Note

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.

Safety Notice

IMPORTANT

Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.

NOTE NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

IMPORTANT is used for comments that add value to the step or procedure being performed.

Safety Information for the JS-5.3 Rotor

This rotor was developed, manufactured, and tested for safety and reliability as part of a
Beckman Coulter centrifuge/rotor system. Their safety or reliability cannot be assured if used in a
centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been
modified without Beckman Coulter’s approval.

Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that such fluids are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) viruses, HIV (I–V), atypical mycobacteria, and certain systemic fungi—further emphasize the
need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this rotor without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.

PN J-TB-089AG

iii

Safety Notice

Safety Information for the JS-5.3 Rotor

Although rotor components and accessories made by other manufacturers may fit in the JS-5.3
rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other
manufacturers’ components or accessories in the JS-5.3 rotor may void the rotor warranty and
should be prohibited by your laboratory safety officer. Only the components
and accessories listed in this publication should be used in this rotor.

Hook all four buckets, loaded or empty, to the rotor for every run. Make sure that filled containers
are loaded symmetrically into the rotor and that opposing labware is filled to the same level with
liquid of the same density.

The rotor and accessories are not designed for use with materials capable of developing flammable
or explosive vapors. Do not centrifuge such materials in nor handle or store them near the
ultracentrifuge.

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor.
Apply appropriate decontamination procedures to the centrifuge and accessories.

Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section on and
derate the run speed as appropriate.

Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins
in scratches and may open fissures in the rotor with continued use.

iv

PN J-TB-089AG

Safety Notice,iii

Alerts for Warning, Caution, Important, and Note, iii

Safety Information for the JS-5.3 Rotor,iii

JS-5.3 Swinging-Bucket Rotor,1

Specifications,1

Description,2

Preparation and Use,2

Prerun Safety Checks,3
Installing the Rotor Yoke,3
Loading the Buckets,5
Operation,10
Removal and Sample Recovery,10
Labware,11
Run Speeds,14

Contents

Care and Maintenance,16

Maintenance,16
Cleaning,18
Decontamination,19
Sterilization and Disinfection,20
Storage,20

Returning a Rotor,21

Supply List,22

Replacement Rotor Parts,22
Supplies,22

Beckman Coulter, Inc.
J Series Swinging-Bucket Rotor Warranty

Related Documents

v

Illustrations

Illustrations

1 Rotor Drive Pin Location and Orientation,3
2 Symmetrical and Balanced Loading,6
3 Correct and Incorrect Alignment of Microplates in the Bucket,8
4 Tube Height Limits for Certain Tube Positions in Adapter

392071,8

5 Tube Height Limits for Certain Tube Positions in Adapter

392072,8

6 Orientation of Capped Tubes in Corner Positions of Adapter

392074,9
7 Bucket Lubrication Area,17
8 Aeroseal Cover,19

vi

Tables

1 Microplates Used in the JS-5.3 Rotor,12
2 Tube and Bottle Adapters for the JS-5.3 Rotor,13
3 Relative Centrifugal Fields for the JS-5.3 Rotor,15

Tables

vii

Tables

viii

JS-5.3 Swinging-Bucket Rotor

r

min

r

av

r

max

r

min

r

av

r

max

1

1

Specifications

Maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5300 RPM

Critical speed range

Density rating at maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL

Relative Centrifugal Fields

Deep-well Plates

At r

max

At r

(179.6 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5650 ´ g

av

At r

min

500-mL Conical Bottles

At r

max

At r

(155.6 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4900 ´ g

av

At r

min

Conditions requiring speed reductions . . . . . . . . . . . . . . . . . . see Run Speeds

k factor

Deep-well plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1536

500-mL conical bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7728

Maximumallowable imbalance of opposing loads. . . . . . . . . . . . . . . 10 grams

Maximum load per bucket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1040 grams

Number of buckets. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Available labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 1 and Table 3

Approximate acceleration time to maximum speed

(fully loaded). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 min

Approximate deceleration time from maximum speed

(fully loaded). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

1. Axis of Rotation

Weight of fully loaded rotor . . . . . . . . . . . . . . . . . . . . . . . . . 14.03 kg (30.91 lb)

Rotor material. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . aluminum

a

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 500 to 1200 RPM

b

at maximum speed

(194.8 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6130 ´ g

(164.3 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5170 ´ g

c

(218.4 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6870 ´ g

(92.7 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2920 ´ g

3

/4 min

a. The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing thrugh the critical

speed range is characterized by some vibration.

b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rw

gravity (g) according to the following formula: RCF = rw

second (2 p RPM /60), and g is the standard acceleration of gravity (9807 mm/s

c. Check manufacturer’s specifications for maximum allowed g force of bottles.

PN J-TB-089AG

2

2

/g — where r is the radius in millimeters, w is the angular velocity in radians per

2

). After substitution: RCF = 1.12r (RPM/1000)

) to the standard acceleration of

2

1-1

JS-5.3 Swinging-Bucket Rotor

Description

Description

Beckman Coulter JS-5.3 rotors are manufactured in a facility that maintains certifications to both
ISO 9001:2008 and ISO 13485:2003. They are for use with the specified Beckman Coulter centrifuges.

The JS-5.3 is a four-place swinging-bucket rotor used in Beckman Coulter Avanti JXN-26, J-26S XP
series, Avanti J-26 XP series, Avanti

J-E, and discontinued J-20 XP series centrifuges. Each rotor
bucket can carry a 96-well kit for high-throughput processing (such as a DNA or RNA kit), or
standard microplates used in the serial dilution of small liquid volumes— up to six stacked (not to
exceed 83.2 mm/3.28 in.) 96-well polypropylene plates, two (stacked) deep-well plates, or one
square-well plate per bucket. An array of adapters carry a wide range of tube and bottle sizes
(from 1.5 to 500 mL).

The rotor yoke is made of aluminum and is black-anodized for corrosion protection. Blue-anodized
aluminum buckets can be run by placing them over pivot pins on the arms of the yoke; they
swing out to horizontal position during centrifugation. A tie-down knob secures the rotor to the
centrifuge drive hub.

Aeroseal covers, made of transparent polyphenysulfone (PPSU) are available. These covers have
been tested

*

to demonstrate containment of microbiological aerosols under normal conditions of
the associated Beckman Coulter rotors and centrifuges when used and maintained as instructed. In
the event of labware leakage, the covers will contain liquids and broken labware, reducing the need
to clean the centrifuge chamber and allowing you to take appropriate precautions before removing
the covers.

Each cover requires a replaceable silicone rubber O-ring that seats around the outside edge of the
cover. An air-vent filter allows passage of air, but not of liquids or aerosols larger than 0.3 micron,
in and out of the bucket to minimize the effects of vacuum conditions inside the centrifuge during
operation. The covers are held in place by attached latches.

Refer to the Warranty at the back of this manual for warranty information.

Preparation and Use

Specific information about the JS-5.3 rotor is given here. Use the J Series Rotors and Tubes Manual
(JR-IM) along with this rotor manual for complete rotor and accessory operation.

NOTE

1-2

Although rotor components and accessories made by other manufacturers may fit in the JS-5.3 rotor,
their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’
components or accessories in the JS-5.3 rotor may void the rotor warranty and should be prohibited by
your laboratory safety officer. Only the components and accessories listed in this publication should be
used in this rotor.

* Validation of microbiological containment was done at an independent third-party testing facility, Health Protection

Agency, Porton Down. Improper use or maintenance may affect seal integrity and thus containment.

PN J-TB-089AG

Prerun Safety Checks

BE

C

K

M

A

N

C

O

U

LTE

R

Rotor Pins

Beckman Coulter
Name

Read the Safety Notice page at the front of this manual before using the rotor.

1

Make sure that the rotor and buckets are clean and show no signs of corrosion or cracking.

If any evidence of damage is present, do not centrifuge the rotor.

2

Check the chemical compatibilities of all materials used.

Refer to Chemical Resistances (publication IN-175).

3

Verify that the labware being used is listed in Table 1 or Table 2.

Installing the Rotor Yoke

JS-5.3 Swinging-Bucket Rotor

Preparation and Use

Two metal drive pins inside the rotor drive hole engage with teeth on the centrifuge hub to prevent
the rotor from slipping during acceleration and deceleration. The drive pins are positioned parallel
to the rotor yoke arm on which

BECKMAN COULTER is engraved (see Figure 1). Note the drive pin

orientation as you follow the steps below.

Figure 1 Rotor Drive Pin Location and Orientation

1

Rotate the yoke until the pins are either parallel to or perpendicular to the drive spindle
hub teeth.

The drive pins may rest either next to or between the drive hub teeth.

PN J-TB-089AG

1-3

JS-5.3 Swinging-Bucket Rotor

Preparation and Use

2

Carefully lower the rotor straight down onto the centrifuge drive hub.

CAUTION

Carefully lower the rotor yoke straight down onto the drive spindle hub to avoid
bending the drive spindle/hub assembly. Do not drop the rotor onto the hub.

3

Slowly rotate the rotor yoke to make sure it is properly seated.

In rare cases, the rotor pins may rest on top of the hub teeth, preventing the yoke from being
secured to the drive hub.

Turning the yoke slightly after installing it will ensure that the rotor is correctly seated.
Lift the rotor yoke slightly while turning it.

4

Tighten the tie-down knob. The tie-down knob will not engage with the drive hub if the drive
pins are on top of the hub teeth.

WARNING

Securely tighten the tie-down knob to attach the rotor yoke to the centrifuge drive
spindle hub. If the rotor is left in the centrifuge between runs, make sure the rotor
is seated on the drive hub and the tie-down knob is tight before each run.

NOTE

Do not remove the tie-down knob from the rotor.

1-4

PN J-TB-089AG

Loading the Buckets

For runs at other than room temperature, refrigerate or warm the rotor and precool the centrifuge
beforehand for fast equilibration.

WARNING

Handle body fluids with care because they can transmit disease. No known test
offers complete assurance that such fluids are free of micro-organisms. Some of
the most virulent—Hepatitis (B and C) viruses, HIV (I–V), atypical mycobacteria,
and certain systemic fungi—further emphasize the need for aerosol protection.
Handle other infectious samples according to good laboratory procedures and
methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment.

Do not run toxic, pathogenic, or other hazardous materials in this rotor without
taking all appropriate safety precautions. Biosafe containment should be used
when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.

JS-5.3 Swinging-Bucket Rotor

Preparation and Use

Symmetrical and Balanced Loading

To ensure optimal performance and stability, the rotor must be loaded symmetrically (see Figure 2).
Two factors affect symmetric loading:

• The buckets must be loaded symmetrically with respect to their pivotal axes.

• The rotor should be loaded symmetrically with respect to its center of rotation.

For best results, load opposing buckets with similar adapter or carrier types and the same type of
labware containing the same amounts of fluid of equal density (within 10 grams). To prevent
imbalance and reduce centrifuge drive wear, the weight of adjacent buckets should be within
220 grams. The maximum load for buckets is 1040 grams each.

During a run, buckets swing 90 degrees from their at-rest position. The pivotal axis of a bucket can
be imagined as a line extending across the bucket from one pivot pin to the other. If a bucket is
loaded so that its weight is unequally distributed on either side of its pivotal axis, it will not hang
vertically at rest and, more importantly, may not swing to a horizontal position during a run. As a
result, extra stress will be placed on the bucket, adapter, carrier, tubes, and/or multiwell plates
during the run, increasing the possibility of breakage or rotor imbalance.

In multiwell plates, samples should be loaded into the wells symmetrically with respect to the
pivotal axis of the carrier (the pivotal axis runs parallel to the crossbar), and opposing buckets
should contain similar loads (see Figure 2).

PN J-TB-089AG

1-5

JS-5.3 Swinging-Bucket Rotor

Examples of Symmetrically Loaded Trays

(Load Opposite Trays the Same Way)

Example of

Nonsymmetrically

Loaded Tray

Examples of Symmetrically Loaded Adapters

Preparation and Use

Figure 2 Symmetrical and Balanced Loading*

* All four positions must have a bucket attached for every run.

1-6

PN J-TB-089AG

JS-5.3 Swinging-Bucket Rotor

1

2

Preparation and Use

Using Buckets

You can load buckets before or after they are installed on the rotor yoke. In either case, we
recommend filling the appropriate labware first and then loading the labware into the buckets to
avoid tripping the imbalance detector.

NOTE

1

When using stacked polypropylene microplates, place a support pad (369382) beneath the bottom
plate and place a cap strip between plates to prevent breakage during centrifugation. Use the support
pad beneath all polystyrene plates.

Tube/Bottle Adapters

Insert the filled labware into the adapter or carrier. (See page 11 for labware information.

1. Support Pad

2. Microplate Carrier

2

Load the filled adapters or carriers into the buckets.

3

If using bucket covers, follow the procedure below under Using Covers.

4

Attach each bucket to the yoke by aligning the grooves in the bucket sides with the pivot pins,
then sliding the buckets down until the pivot pins are seated in the bucket pockets.

5

Gently swing the buckets to ensure that they are properly seated on the pivot pins.

PN J-TB-089AG

1-7

JS-5.3 Swinging-Bucket Rotor

Preparation and Use

Using Covers

NOTE Covers can be used only with blue buckets (368706). They cannot be used with previously

manufactured black buckets (368415).

Always visually check to make sure that the labware fits under the cover before your attempt to
secure the latches. In addition, note the following information for specific components.

• When using six stacked polystyrene microplates, make sure that the plates are aligned parallel
to the bucket edge, as shown in Figure 3, before placing a cover on the bucket.

Figure 3 Correct and Incorrect Alignment of Microplates in the Bucket

Correct Incorrect

• All adapters listed in Table 2 can be used with covers except 500-mL conical adapter 392078.

• Adapters 392071, 392072, and 392074 have the following tube height limits.
— In adapter 392071, the maximum height of tubes placed in the four corner positions of the

adapter (shown shaded in Figure 4) is 90 mm. Tubes up to 105.4 mm tall can be placed in all
other positions.

Figure 4 Tube Height Limits for Certain Tube Positions in Adapter 392071

— In adapter 392072, the maximum height of tubes placed in the end positions of the middle

row (shown shaded in Figure 5) is 104 mm. Tubes up to 114 mm tall can be placed in all
other positions.

Figure 5 Tube Height Limits for Certain Tube Positions in Adapter 392072

1-8

PN J-TB-089AG

JS-5.3 Swinging-Bucket Rotor

Leave Clear

Preparation and Use

— In adapter 392074, capped tubes placed in the four corner positions must be oriented

so that the cap hinges and tabs do not extend towards the corners of the adapter
(see Figure 6).

Figure 6 Orientation of Capped Tubes in Corner Positions of Adapter 392074

Install the covers as follows.

CAUTION

When centrifuging hazardous materials, load and unload buckets and install and
remove Aeroseal covers under an appropriate hood or biological safety cabinet.

1

Make sure that the bucket and bucket cover surfaces are clean, dry, and undamaged.

2

Make sure that the O-ring (368703) is in good condition and lightly coated with silicone vacuum
grease (335148).

3

Hold the cover with both hands, holding the latches up, and place the cover on the bucket.

Push down on all four corners at once to properly seat the cover.
Look at the bucket from the side to make sure that the cover is centered on the bucket.

4

Steady the bucket with one hand, and with the other hand, snap one latch down.

Then snap the second latch down.

Be careful not to pinch your fingers as you fasten the latches.

5

Look at the bucket/cover interface, checking to make sure that the red O-ring is not protruding
at the top of the bucket.

If you can see a portion of the O-ring above the bucket top edge, release the latches and remove
the cover.

Then repeat steps 3 and 4 to reseal the assembly.

PN J-TB-089AG

1-9

JS-5.3 Swinging-Bucket Rotor

Preparation and Use

Operation

Temperatures may vary slightly between centrifuges. If sample temperature is crucial, test temperature
settings on your instrument using water samples. For runs at other than room temperature, refrigerate or
warm the rotor beforehand for fast equilibration.

1

Refer to the instrument instruction manual for centrifuge operation.

2

See Run Speeds, for information about speed limitations.

Removal and Sample Recovery

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply appropriate decontamination procedures to the
centrifuge and accessories.

Covers can be removed while the buckets are in the centrifuge, or buckets can be placed on a
benchtop before the covers are removed. If hazardous materials have been centrifuged, place the

buckets under an appropriate hood or biological safety cabinet before removing the covers.

To remove covers while the buckets are in the centrifuge:

1

Carefully release the latches, one at a time, keeping the bucket stable with one hand.

Alternately, place your thumb and/or fingers on the edge of the cover and lift up.
A number of different hand positions can be used.
Find one that is comfortable and provides adequate leverage.
See the Note below if you cannot remove a cover.

2

Gently lift up one of the latches to break the seal formed during centrifugation.

3

Remove the cover, being careful not to disturb the bucket contents.

4

Remove the labware from the bucket.

1-10

PN J-TB-089AG

JS-5.3 Swinging-Bucket Rotor

Preparation and Use

To remove covers from buckets placed on a benchtop:

1

Place an absorbent pad on the benchtop to increase bucket stability on the benchtop surface.

2

Remove a bucket from the rotor and place the bucket on the pad.

3

Carefully release the latches, one at a time.

Lift up on one latch to break the seal.
See the Note below if you cannot remove a cover.

4

Remove the cover, being careful not to disturb the bucket contents

5

Remove the labware from the buckets.

Labware

6

If removing the rotor yoke, loosen the tie-down knob and lift the yoke straight up and off the
drive hub.

NOTE

If a cover is difficult to remove, wait 1 to 2 minutes, then try again to lift the cover off the bucket. If
this doesn’t work, try changing hand positions and lifting again. If required, use a plastic spatula to lift the
cover off the bucket. Do not use a metal tool that could damage the O-ring or bucket. To prevent covers
from sticking, do the following between runs.

• Lubricate the O-rings with a thin coat of silicone vacuum grease (335148).

• Inspect the air-vent filters before each run to make sure that they are clean. If a filter is discolored,

replace it.

Temperature Limits

• Beckman Coulter plastic adapters and carriers have been centrifuge tested for use at
temperatures between 2 and 40°C. For centrifugation at other temperatures, pretest labware
under anticipated run conditions.

• If plastic containers are frozen before use, make sure that they are thawed to at least 2°C prior
to centrifugation.

Labware

Use the labware listed in Table 1 or Table 2 in the buckets.

NOTE RCF limits in Ta b l e 2 are the tested limits for the adapters only. Tubes and bottles used may require

lower run speeds. Refer to manufacturers’ recommendations and test labware before use.

PN J-TB-089AG

1-11

JS-5.3 Swinging-Bucket Rotor

Preparation and Use

Table 1 Microplates Used in the JS-5.3 Rotor

Description Volume

multiwell polystyrene plate, 96-well,
nonsterile

300 mL/well 609844

deep-well polystyrene plate,
96-well, nonsterile (do not stack

1 mL/well 267001

these plates)

deep-well polystyrene plate,
96-well, sterile (do not stack these

1 mL/well 267004

plates)

a

Part

Number

Accessory

Description

b

cap strip, nonsterile

Part

Number

c

267002

(pkg/12)

cap strip, sterile

c

267005

(pkg/12)

aluminum foil lid

d

538619

(pkg/100)

c

cap strip, nonsterile

267002

(pkg/12)

cap strip, sterile

c

267005

(pkg/12)

aluminum foil lid

d

538619

(pkg/100)

c

cap strip, nonsterile

267002

(pkg/12)

cap strip, sterile

c

267005

(pkg/12)

aluminum foil lid

d

538619

(pkg/100)

c

cap strip, nonsterile

267002

(pkg/12)

deep-well polypropylene plate,
96-well, nonsterile

1 mL/well 267006

cap strip, sterile

aluminum foil lid

c

d

267005

(pkg/12)

538619

(pkg/100)

c

cap strip, nonsterile

267002

(pkg/102)

deep-well polypropylene plate,
96-well, sterile

1 mL/well 267007

cap strip, sterile

aluminum foil lid

c

d

267005

(pkg/12)

538619

(pkg/100)

square-well polypropylene plate 2 mL/well 140504

a. High-throughput processing kits are available commercially; observe manufacturer’s recommendations for speed and

temperature limitations.

b. When stacking polypropylene multiwell plates, place a support pad (369382) beneath the bottom plate and a cap strip

between the plates to prevent breakage during centrifugation. Also use the support pad beneath all polystyrene
deep-well plates.

c. Caps are optional.

d. Requires 4-inch soft-rubber roller (538618) for installation.

aluminum foil lid

d

538619

(pkg/100)

1-12

PN J-TB-089AG

Table 2 Tube and Bottle Adapters for the JS-5.3 Rotor

Part

Number

(Pkg/4)

Adapter

Adapter

Color

Tube/Bottle

Size

JS-5.3 Swinging-Bucket Rotor

Preparation and Use

Maximum

Tubes/

Bottles per

Adapter

RCF at Max

Speed
(RCF)

a

beige

purple

392071

392072

b

b

13 mm dia. 33 6145 ´ g

16 mm dia. 24 6145 ´ g

red 392073 17 mm dia. 20 6145 ´ g

yellow

green 392075 15 mL conical 18

392074

b

29 mm dia. 8 6145 ´ g

6425 ´ g

c

PN J-TB-089AG

black 392076 50 mL conical 7 6480 ´ gc

orange 392079 250 mL conical 1 6870 ´ g

yellow 392077

250 mL round/

230 mL conical

d

16670 ´ g

1-13

JS-5.3 Swinging-Bucket Rotor

reduced maximum speed = (5300 RPM)

1040 grams

heaviest load in grams

------------------------------------------ -------------

Preparation and Use

Table 2 Tube and Bottle Adapters for the JS-5.3 Rotor (Continued)

Adapter

Adapter

Color

blue

Part

Number

(Pkg/4)

392078

Maximum

Tubes/

Tube/Bottle

Size

e

500 mL conical 1 6870 ´ g

Bottles per

Adapter

RCF at Max

Speed

a

(RCF)

Run Speeds

The centrifugal force at a given radius in a rotor is a function of speed. Comparisons of forces
between different rotors are made by comparing the rotors’ relative centrifugal fields (RCF). When
rotational speed is adjusted so that identical samples are subjected to the same rcf in two different
rotors, the samples are subjected to the same force (see Table 3 for examples).

yellow

yellow

a. Maximum RCF for adapters. Use manufacturers’ recommendations for tube and bottle limits.

b. Observe limitations described on page 8 when using bucket covers with these adapters.

c. Maximum RCF for labware in this adapter is 40000 ´ g.

d. Requires a cushion (356983) to be placed at bottom of adapter.

e. Cannot be used with bucket cover.

f. Use this adapter inside the 250-mL adapter (392077) to run tubes at 6670 ´ g.

356966

(each)

356964

(each)

50 mL conical

15 mL conical

f

f

16670 ´ g

46670 ´ g

If the weight of the load in a bucket exceeds 1040 grams, or if the solution density is more than

1.2 g/mL, reduce the maximum allowable run speed according to the following equation:

Do not select rotational speeds in excess of 5300 RPM.

NOTE

1-14

Polystyrene plates are subject to cracking at high speeds. Test polystyrene plates using water in place

of sample to determine required speed deration.

PN J-TB-089AG

JS-5.3 Swinging-Bucket Rotor

RCF (x g)

r

max

r

min

r

av

Speed (RPM)

Relative Centrifugal Fields for the JS-5.3 Rotor with Deep-well Plates

0

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

5500

6000

6500

2000 3000 4000 5000 5300

10000

Preparation and Use

Table 3 Relative Centrifugal Fields for the JS-5.3 Rotor

Deep-well Plates 500-mL Conical Bottles

Relative Centrifugal Field ´ g

Rotor
Speed
(RPM)

5300
5000
4750
4500
4250
4000

3750
3500
3250
3000
2750
2500

2250
2000
1750
1500
1000

750
500

At r

max

(194. mm)

6130
5450
4920
4420
3940
3490

3070
2670
2300
1960
1650
1360

1110

873
668
491
218
115

55

At r

av

(179.6 mm)

5650
5030
4540
4070
3630
3220

2830
2460
2130
1810
1520
1260

1020

805
616
453
201
103

50

At r

min

(164.3 mm

5170
4600
4150
3730
3320
2940

2590
2250
1940
1660
1400
1150

932
736
564
414
184
108

46

a

b

Relative Centrifugal Field ´ g

Rotor
Speed
(RPM)

5300
5000
4750
4500
4250
4000

3750
3500
3250
3000
2750
2500

2250
2000
1750
1500
1000

750

0

At r

max

(218.4 mm)

6870
6120
5520
4950
4420
3910

3440
3000
2590
2200
1850
1530

1240

978
749
550
245
138

0

At r

av

(155.6 mm)

4900
4360
3930
3530
3150
2790

2450
2140
1840
1570
1320
1090

882
697
534
392
174

98

0

At r

min

(92.7 mm)

2920
2600
2340
2200
1880
1660

1460
1270

1100

934
785
649

526

415
318
234
104

58

0

a. Entries in the table are calculated from the formula RCF = 1.12r (RPM/1000)2 and then rounded to three significant digits.

b. Refer to manufacturers’ recommendations for maximum RCF limits of bottles.

PN J-TB-089AG

1-15

JS-5.3 Swinging-Bucket Rotor

0

1000

2000

3000

4000

5000

6000

7000

8000

0 1000 2000 3000 4000 5000 5300

RCF (x g)

r

max

r

min

r

av

Speed (RPM)

Relative Centrifugal Fields for the JS-5.3 Rotor with 500-mL Conical Bottles

Care and Maintenance

Care and Maintenance

Maintenance

Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins in
scratches and may open fissures in the rotor with continued use.

1

2

Periodically (at least monthly) inspect the rotor yoke and buckets, especially inside cavities, for
rough spots or pitting, cracks, white powder deposits—frequently aluminum oxide—or heavy

discoloration.
If any of these signs are evident, do not run the rotor.
Contact your Beckman Coulter representative for information about the Field Rotor Inspection

Program and the rotor repair center.

Before using the rotor, inspect the rotor drive pins to ensure that they are not damaged.

Damaged drive pins can prevent the rotor from seating properly on the centrifuge drive hub
(Avanti J series centrifuges only).

To inspect the drive pins, turn the rotor upside down and look into the drive hole in the center
of the rotor.

If the drive pins appear damaged, contact Beckman Coulter Field Service.

3

Before using the rotor tie-down knob (368410), check it for damage such as distortion, splitting,

1-16

or stripped threads.
Replace it if it is damaged.

PN J-TB-089AG

JS-5.3 Swinging-Bucket Rotor

Lubrication Area

(both pin sockets,

all buckets)

BECKMAN

MADE IN

COULTER

U.S.A.

JS-5.3

MAX 5.300 RPM

Care and Maintenance

4

Before each use, inspect the covers to make sure they are in good condition.

Do not use a cover that is cracked or damaged.
Inspect each O-ring to make sure that is smooth and free of nicks, tears, and abrasions.
Make sure that it has not stretched beyond its original shape and size.
Inspect the top edge of the rotor bucket to make sure that is clean and smooth.

5

Inspect the bucket cover air-vent filters before each run to make sure that they are clean.

If a filter is discolored, replace it by gently pushing it out from underneath the cover with a
pencil or other non-metal tool that will not scratch the cover material.

Insert a new air-vent filter into each cover.
An audible snap will be heard as the filter is inserted.
When properly inserted, the filter will sit slightly (0.76 mm) above the cover surface.

6

Approximately once a week, and after cleaning and/or autoclaving, wipe the rotor pins and pin
sockets (see Figure 7) with a paper towel, then coat pin sockets with Paint On Graphite

Lubricant (977212).
Allow the lubricant to dry for at least 5 minutes before installing the rotor in a centrifuge.

Figure 7 Bucket Lubrication Area

7

Apply a light film of powder, such as talcum powder, to the tube adapter rubber bases after
cleaning or as required to prevent sticking.

PN J-TB-089AG

1-17

JS-5.3 Swinging-Bucket Rotor

Care and Maintenance

8

Store the rotor in a dry environment (not in the centrifuge).

Refer to Chemical Resistances (publication IN-175) for the chemical compatibilities of rotor and
accessory materials.

Your Beckman Coulter representative provides contact with the Field Rotor Inspection
Program and the rotor repair center.

Cleaning

Wash rotor components immediately if salts or other corrosive materials are used or if spillage has
occurred. Do not allow corrosive materials to dry on the rotor.

Under normal use, wash the rotor frequently (at least weekly) to prevent buildup of residues.

NOTE

1

2

3

4

Do not wash the rotor components in a dishwasher. Do not soak the rotor in detergent solution for long

periods, such as overnight.

Wash the rotor yoke, buckets, adapters, and microplate carriers in a mild detergent, such as
Solution 555
Dilute the detergent with water (10 parts water to 1 part detergent).
The Rotor Cleaning Kit (339558) contains two plastic-coated brushes and two quarts

of Solution 555 for use with rotors and accessories.

Thoroughly rinse the cleaned rotor components with distilled water.

Air-dry the rotor components upside down.

Do not use acetone to dry the rotor.

When adapters are dry, apply a light film of powder, such as talcum powder, to the rubber bases
to prevent sticking.

(339555), that won’t damage the rotor.

5

When the rotor is dry, lubricate the bucket sockets and pins as described under Maintenance.

Cleaning Bucket Covers

The covers and O-rings should be cleaned at least weekly under normal use. Remove and discard the
filter before cleaning. Insert a new filter after cleaning and before the next use.

1-18

PN J-TB-089AG

JS-5.3 Swinging-Bucket Rotor

O-ring

Air-vent filter

Care and Maintenance

1

Remove the air-vent filters (as described in Maintenance, above) and O-rings from the covers
(see Figure 8).

Figure 8 Aeroseal Cover

2

Wash the covers and O-rings with a mild detergent such as Solution 555 (339555), diluted with
water (10 parts water to 1 part detergent).

Do not wash the components in a dishwasher.

3

Thoroughly rinse the cleaned components with water and air-dry upside down.

Do not use acetone to dry the components.

4

Insert a new air-vent filter into each cover, as described in Maintenance, above.

Decontamination

If aluminum rotor components become contaminated with radioactive material, decontaminate
them using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a
number of solutions and found two that do not harm anodized aluminum: RadCon Surface Spray or

IsoClean Solution (for soaking),

*

and Radiacwash.

†

* In U.S.A., contact Nuclear Associates (New York); in Eastern Europe and Commonwealth States, contact Victoreen GmbH

(Munich); in South Pacific, contact Gammasonics Pty, Ltd. (Australia); in Japan, contact Toyo Medic Co. Ltd. (Tokyo).

† n U.S.A., contact Biodex Medical Systems (Shirley, NY); internationally, contact the U.S. office to find the dealer nearest you.

PN J-TB-089AG

1-19

JS-5.3 Swinging-Bucket Rotor

Care and Maintenance

While Beckman Coulter has tested these materials and found that they do not damage components,
no guarantee of decontamination is expressed or implied. Follow appropriate decontamination
procedures as directed by your laboratory safety officer.

If the rotor or other components are contaminated with toxic or pathogenic materials, follow
appropriate decontamination procedures as directed by your laboratory safety officer. Refer to
the Chemical Resistances (publication IN-175) to ensure that procedures will not damage the
components.

Sterilization and Disinfection

• The rotor can be autoclaved at 121°C for up to an hour. Plastic parts can be autoclaved at 121°C
for up to 30 minutes. Place the rotor yoke, buckets, and/or microplate carriers in the autoclave
upside down.

• The bucket cover, with air-vent filter removed, can be autoclaved at 121°C for up to 30 minutes.
Before autoclaving, remove the filter from each cover as described in Maintenance, above. After
autoclaving, insert a new air-vent filter into each cover.

• Ethanol (70%) or hydrogen peroxide (6%) may be used on all rotor components, including those
made of plastic. Bleach (sodium hypochlorite) may be used, but may cause discoloration of
anodized surfaces. Use the minimum immersion time for each solution, per laboratory
standards. Cold sterilization methods such as ethanol (70%), hydrogen peroxide (10%),

Wescodyne, or Cidex
any other sterilization methods.

*

may be used on bucket covers. Consult Chemical Resistances before using

Storage

CAUTION

Ethanol is a flammability hazard. Do not use it in or near operating centrifuges.

using any other sterilization methods.

While Beckman Coulter has tested these methods and found that they do not damage the rotor or
components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or
disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

When it is not in use, store the rotor in a dry environment (not in the centrifuge).

* Cidex is a gluteraldehyde (1,5-pentanedial) product.

1-20

PN J-TB-089AG

Returning a Rotor

Before returning a rotor or accessory for any reason, prior permission must be obtained from
Beckman Coulter, Inc. A return form may be obtained from your local Beckman Coulter sales office.
The form should contain the following information:

• rotor type and serial number,

• history of use (approximate frequency of use),

• reason for the return,

• original purchase order number, billing number, and shipping number, if possible,

• name and email address of the person to be notified upon receipt of the rotor or accessory at

the factory,

• name and email address of the person to be notified about repair costs, etc.

To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from
pathogens and/or radioactivity. Sterilization and decontamination must be done before returning
the parts. Smaller items (such as tubes, bottles, etc.) should be enclosed in a sealed plastic bag.

JS-5.3 Swinging-Bucket Rotor

Returning a Rotor

All parts must be accompanied by a note, plainly visible on the outside of the box or bag, stating that they
are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach

this notification will result in return or disposal of the items without review of the reported problem.

Use the address label printed on the return form when mailing the rotor and/or accessories.

Customers located outside the United States should contact their local Beckman Coulter office.

PN J-TB-089AG

1-21

JS-5.3 Swinging-Bucket Rotor

Supply List

Supply List

NOTE Publications referenced in this manual can be obtained at www.beckmancoulter.com, by

calling Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local
Beckman Coulter office.

See the Beckman Coulter High Performance, High Speed, High Capacity Rotors Tubes & Accessories
catalog (BR-8102, available at www.beckmancoulter.com) for detailed information on ordering
parts and supplies. For your convenience, a partial list is given below.

Replacement Rotor Parts

JS-5.3 rotor assembly 368690

Rotor tie-down knob 368410

Description Part Number

Supplies

Bucket, blue (set of 4) 368706

Containment cover set

(includes 2 covers with pre-installed O-rings and air-vent
filters, plus 2 each replacement O-rings and filters)

O-ring replacement set (qty/4) 368703

Air-vent filter replacement set (qty/60) 368148

Microplate carrier kit

Contains:

Microplate carrier (set of 4)
Support pad (set of 4)

368417

368914

368905
369382

NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.

Description Part Number

Labware see Ta b l e 1 and Ta b l e 2

Replacement tube and bottle adapters (set of 2)

Beige (13 mm dia)
Purple (16 mm dia)
Red (17 mm dia)
Yellow (29 mm dia)
Green (15 mL conical)
Black (50 mL conical)
Orange (250 mL conical)
Yellow (250 mL round/230 mL conical)
Blue (500 mL conical)

368907
368909
368910
368911
368915
368916
369385
369383
369384

Rubber roller, 4-in., for sealing foil microplate lids 538618

1-22

PN J-TB-089AG

Description Part Number

Rotor Cleaning Kit 339558

Solution 555 (1 qt) 339555

Rotor cleaning brush 339379

Paint On Graphite Lubricant (1/2 oz) 977212

Silicone vacuum grease (1 oz) 335148

JS-5.3 Swinging-Bucket Rotor

Supply List

PN J-TB-089AG

1-23

JS-5.3 Swinging-Bucket Rotor

Supply List

1-24

PN J-TB-089AG

Beckman Coulter, Inc.

years

7

--------------

J Series Swinging-Bucket Rotor Warranty

Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc.,
terms and conditions of sale in effect at the time of sale, Beckman Coulter, Inc. agrees to correct
either by repair, or, at its election, by replacement, any defects of material or workmanship
which develop within seven (7) years after delivery of a J series rotor to the original buyer by
Beckman Coulter, Inc. or by an authorized representative, provided that investigation and factory
inspection by Beckman Coulter discloses that such defect developed under normal and proper use.
Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this
warranty, Beckman Coulter will supply free of charge all centrifuge parts required for repair.

Replacement

Any product claimed to be defective must, if requested by Beckman Coulter be returned to the
factory, transportation charges prepaid, and will be returned to Buyer with the transportation
charges collect unless the product is found to be defective, in which case Beckman Coulter will pay
all transportation charges.

A defective rotor will be replaced by Beckman Coulter at its then current list price less a credit based
upon the age of the rotor (years since date of purchase). The Buyer shall not receive credit until the
claimed defective rotor is returned to Beckman Coulter’s Indianapolis, Indiana facility or delivered
to a Beckman Coulter Field Service representative.

The replacement price (cost to Buyer) for the respective rotor shall be calculated as follows:

Replacement price = Current rotor list price ×

Conditions

1. Except as otherwise specifically provided herein, this warranty covers the rotor only and

Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including
but not limited to (i) tubes, (ii) tube caps, (iii) tube adapters, or (iv) tube contents.

2. This warranty is void if the rotor has been subjected to customer misuse such as operation or
maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual.

3. This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge
unmatched to the rotor characteristics, or is operated in a Beckman Coulter centrifuge that has
been improperly disassembled, repaired, or modified.

Disclaimer

IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES
OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER,
INC. SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND
WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR,
MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.

PN J-TB-089AG

Warranty-1

Beckman Coulter, Inc. J Series Swinging-Bucket Rotor Warranty

Warranty-2

PN J-TB-089AG

1

A

B

2

C

3

D

E

F

G

H

4

5

6

7

8

9

10

11

12

www.beckmancoulter.com

Related Documents

Rotors and Tubes for
Beckman Coulter J2, J6,and
Avanti J Series Centrifuges

PN JR-IM-10

• Rotors

• Tubes, Bottles, and

Accessories

• Using Tubes and
Accessories

• Using Fixed-Angle Rotors

• Using Swinging-Bucket

Rotors

• Using Vertical-Tube and
Rack Type Rotors

• Care and Maintenance

• Chemical Resistances

• Temperature

Compensation Tables

• Gradient Materials

• Blood Component

Separation

Available in electronic pdf or
CD-ROM by request.

Avanti J-26S XP

PN B10087

Avanti J-26S XPI

PN B10093

Avanti J-26 XP

PN J326XP-IM-5

Avanti J-26 XPI

PN J326XPI-IM-4

Avanti J-E

PN SJ-IM-8

Avanti JXN

PN B38322

PN B38323

Chemical Resistances for
Beckman Coulter
Centrifugation Products

PN IN-175

High Performance, High
Speed, High Capacaity
Rotors, Tubes, & Accessories

PN BR-1802

Available in hard copy by
request.

Available at
www.beckmancoulter.com

© 2014 Beckman Coulter, Inc.

All Rights Reserved