Page 1
Instructions For Use
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
JA-14.50 Fixed-Angle Rotor
For Use in Beckman Coulter Avanti J Series
PN B32164AC
October 2016
Page 2
JA-14.50 Fixed-Angle Rotor
PN B32164AC (October 2016)
© 2016 Beckman Coulter, Inc.
All rights reserved.
Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Contact Us
If you have any questions, contact our Customer Support
Center.
• Worldwide, find us via our website at
www.beckmancoulter.com/customersupport/support.
• In the USA and Canada, call us at 1-800-369-0333.
• Outside of the USA and Canada, contact your local
Beckman Coulter Representative.”
Original Instructions
Page 3
Revision History
For labeling updates, go to www.beckmancoulter.com and download the latest version of the manual or
system help for your instrument.
Issue AC, 07/2016
Rotor, Specifications, Description, Preparation and Use, Tubes, Replacement Rotor Parts, Supplies.
Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin of the
amended page.
— Updates were made to the following sections: Safety Information for the JA-14.50
PN B32164AC
iii
Page 4
Revision History
iv
PN B32164AC
Page 5
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to use this equipment. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to
how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Warning, Caution, and Note
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
Safety Notice
NOTE
NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
PN B32164AC
v
Page 6
Safety Notice
Safety Information for the JA-14.50 Rotor
Safety Information for the JA-14.50 Rotor
This rotor was developed, manufactured, and tested for safety and reliability as part of a
Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a
centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been
modified without Beckman Coulter’s approval.
Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that such fluids are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) viruses, HIV (I-V), atypical mycobacteria, and certain systemic fungi—further emphasize the
need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe
proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive
materials in this rotor without taking appropriate safety precautions. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization Laboratory
Biosafety Manual) are handled; materials of a higher group require more than one level of
protection.
The rotor and accessories are not designed for use with materials capable of developing flammable
or explosive vapors, or a hazardous chemical reaction. Do not centrifuge such materials in nor
handle or store them near the centrifuge.
Although rotor components and accessories made by other manufacturers may fit in the JA-14.50
rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other
manufacturers’ components or accessories in the JA-14.50 rotor, excluding the tubes referenced in
Table 1 and Table 3 may void the rotor warranty and should be prohibited by your laboratory safety
officer. Only the components and accessories listed in this publication should be used in this rotor.
Do not run an empty rotor. Place filled tubes in at least two opposing cavities. Make sure that filled
containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same
level with liquid of the same density.
If disassembly reveals evidence of leakage, and pathogenic or radioactive materials are involved,
you should assume that some fluid escaped the rotor. Apply appropriate decontamination
procedures to the centrifuge and accessories.
Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section on Run
Speeds, and derate the run speed as appropriate.
Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins
in scratches and may open fissures in the rotor with continued use.
vi
PN B32164AC
Page 7
Revision History,iii
Safety Notice,v
Alerts for Warning, Caution, and Note,v
Safety Information for the JA-14.50 Rotor,vi
JA-14.50
Fixed-Angle Rotor,1
Specifications,1
Description,2
Preparation and Use,4
Prerun Safety Checks,4
Rotor Preparation,4
Operation,6
Installing the Rotor,6
Rotor Drive Pin Orientation,6
Rotor Lid Configurations,8
Rotor Installation,9
Run Procedure,10
Removal and Sample Recovery,10
Dual-Locking Lid,10
Single-Locking Lid,11
If the Rotor Sticks to the Drive Hub,12
Tubes,13
Tubes for JA-14.50 Rotors Manufactured before October 2016,13
Tubes for JA-14.50 Rotors Manufactured after October 2016,16
Temperature Limits,17
Run Times,18
Run Speeds,20
Contents
Care and Maintenance,21
Maintenance,21
Cleaning,22
Decontamination,23
Sterilization and Disinfection,24
Storage,24
Returning a Rotor,25
Supply List,25
Replacement Rotor Parts,25
Supplies,26
vii
Page 8
Contents
Beckman Coulter, Inc.
J Series Rotor Warranty
viii
Page 9
Illustrations
1The JA-14.50 Fixed Angle Rotor,2
2 Fluid-Containment Annulus,3
3 Typical Examples of Arranging Sixteen or Fewer Tubes in the
Rotor,5
4 Rotor Drive Pin Location and Orientation,7
5 Centrifuge Drive Hub Configurations,7
6 Installing the Rotor,8
7 Using the Rotor Removal Tool,12
8 Rotor Serial Number with T,13
9 Rotor Lid Serial Number with T,13
Illustrations
10 Examples of Tubes with Minimum and Moderate Crazing,18
11 Relative Centrifugal Fields Chart for the JA-14.50 Rotor,21
ix
Page 10
Tables
Tables
1 Adapters to support tubes from other manufacturers for the JA-
14.50 Rotor manufactured before October 2016,13
2 Available Beckman Coulter Bottles and Tubes for the JA-14.50
Rotor manufactured prior to October 2016,14
3 Adapters to support tubes from other manufacturers for the JA-
14.50 Rotor manufactured after October 2016,16
4 Available Beckman Coulter Bottles and Tubes for the JA-14.50
Rotor manufactured after October 2016 ,17
5 Relative Centrifugal Fields for the JA-14.50 Rotor,20
x
Page 11
JA-14.50
Fixed-Angle Rotor
Specifications
Maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14,000 RPM
a
Critical speed range
Density rating at maximum speed. . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL
Relative Centrifugal Field
at r
(160 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35,000 g
max
at r
(124 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27,100 g
av
at r
(87 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19,100 g
min
k factors at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 787
Conditions requiring speed reductions . . . . . . . . . . . . . see Run Speeds
Number of tube cavities. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Available tubes see Table 1, Table 2, Table 3 and Table 4
Nominal dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 115 mm
Nominal tube capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 mL
Nominal rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 800 mL
Approximate acceleration time to maximum
speed (rotor fully loaded) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Approximate deceleration time from maximum
speed (rotor fully loaded, max. break) . . . . . . . . . . . . . . . . . 2
Rotor weight, unloaded . . . . . . . . . . . . . . . . . . . . . . . 10.5 kg (23 lbs 3 oz.)
Rotor material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . aluminum
1. Axis of Rotation
a. The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing through the critical
speed range is characterized by some vibration.
b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (r
gravity (g) according to the following formula: RCF = r
(2 RPM /60), and g is the standard acceleration of gravity (9807 mm/s
2
/g — where r is the radius in millimeters, is the angular velocity in radians per second
. . . . . . . . . . . . . . . . . . . . . . . . . . . .400 to 1000 RPM
b
at maximum speed
2
) to the standard acceleration of
2
). After substitution: RCF = 1.12r (RPM/1000)
2
½
½
min
min
PN B32164AC
NOTE
At 14,000 RPM and at an ambient temperature of 25°C or below, the minimum rotor temperature is
4°C. Above 38°C ambient, 4°C may not be achievable at 14,000 RPM.
1
Page 12
JA-14.50 Fixed-Angle Rotor
Description
Description
Beckman Coulter JA-14.50 rotors are manufactured in a facility that maintains certifications to both
ISO 9001:2008 and ISO 13485:2003. They are for use with the specified Beckman Coulter centrifuges.
The JA-14.50 fixed-angle rotor is designed to hold up to sixteen 50-mL tubes at a 35-degree angle
(see Figure 1). Used in Beckman Coulter Avanti J series centrifuges, the JA-14.50 rotor develops
centrifugal forces that can efficiently pellet cells, bacteria, and food products; separate proteins,
viruses, and subcellular fractions, and do phase separation and binding studies.
1. Dual-Locking Rotor Lid
2. Small O-ring
3. Large O-ring
4. Rotor Body
The JA-14.50 rotor with dual-locking lid was tested
*
to demonstrate containment of microbiological
aerosols under normal operating conditions of the associated Beckman Coulter centrifuge, when
used and maintained as instructed. The rotor is also available with a single-locking lid.
Figure 1 The JA-14.50 Fixed Angle Rotor
1. Single-Locking Lid
2. Rotor Body
3. O-Ring
4. Dual-Locking Lid
The JA-14.50 rotor has a patented fluid-containment annulus, located below the O-ring sealing
surface (see Figure 2). The annulus helps to retain liquid that may escape from leaking or overfilled
tubes, thereby minimizing the escape of liquid into the instrument chamber during centrifugation.
* Validation of microbiological containment was done at an independent third-party testing facility. Improper use or
maintenance may affect seal integrity and thus containment.
2
PN B32164AC
Page 13
JA-14.50 Fixed-Angle Rotor
Description
Figure 2 Fluid-Containment Annulus
1. Annulus
The rotor and lid are made of anodized aluminum. Two O-rings, one in the rotor lid made of Buna-N
rubber, and one in the rotor body made of ethylene propylene rubber, help to maintain atmospheric
pressure inside the rotor during centrifugation when they are properly lubricated. Pins in the rotor
drive hole mate with the centrifuge drive hub to prevent the rotor from slipping during acceleration
and deceleration.
JA-14.50 rotors manufactured after October 2016 have a slightly tapered cell profile, which improves
performance of most commercially available 50 mL conical tubes. However, some tubes and
adapters that were compatible prior to October 2016 are no longer compatible. Please consult Tables
1-4 for compatible tubes and adapters.
NOTE
JA14.50 rotors and lids manufactured after October 2016 include the letter “T” after the serial number
(see Figure 8 and Figu re 9).
See the Warranty at the back of this manual for warranty information.
PN B32164AC
3
Page 14
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Preparation and Use
Specific information about the JA-14.50 rotor is given here. Use the Rotors and Tubes for
Beckman Coulter J Series Centrifuges manual (publication JR-IM-10) and the centrifuge instruction
manual along with this manual for complete rotor and accessory operation information.
NOTE
Although rotor components and accessories made by other manufacturers may fit in the JA-14.50
rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’
components or accessories in the JA-14.50 rotor, excluding the tubes referenced in Ta b l e 1 and Ta b l e 3 ,
may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the
components and accessories listed in this publication should be used in this rotor.
Prerun Safety Checks
Read the Safety Notice section at the front of this manual before using the rotor.
1
Make sure that the rotor, lid, and all tubes or accessories are clean and show no signs of
corrosion or cracking.
2
Verify that the tubes being used are listed in Table 1, Table 2, Table 3 and Table 4.
3
Refer to Chemical Resistances (publication IN-175), included in the Rotors and Tubes CD-ROM for
the chemical compatibilities of all materials used.
Rotor Preparation
For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration.
1
Be sure that metal threads in the rotor are clean and lightly but evenly lubricated with Spinkote
lubricant (306812).
Also ensure that O-rings are lightly but evenly coated with silicone vacuum grease (335148).
2
Load the filled containers symmetrically into the rotor.
• (Refer to Tubes for information about containers.)
• If fewer than sixteen tubes are being run they must be arranged symmetrically in the rotor
(see Figure 3).
• Opposing tubes must be filled to the same level with liquid of the same density.
4
PN B32164AC
Page 15
Figure 3 Typical Examples of Arranging Sixteen or Fewer Tubes in the Rotor
JA-14.50 Fixed-Angle Rotor
Preparation and Use
PN B32164AC
5
Page 16
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Operation
1
Precool the rotor in the centrifuge or in a refrigerator before use—especially before short
runs—to ensure that the rotor reaches the set temperature.
A suggested precooling cycle is a minimum of 30 minutes at 2000 RPM at the required
temperature.
2
If fluid containment is required, use capped tubes.
It is strongly recommended that all containers carrying physiological fluids be capped to
prevent leakage.
3
Select the JA-14.50 rotor.
4
For additional operating information, see the following:
• Run Times for using k factors to adjust run durations.
• Run Speeds for information about speed limitations.
Installing the Rotor
Rotor Drive Pin Orientation
Two metal pins inside the rotor drive hole engage with teeth on the centrifuge drive hub to prevent
the rotor from slipping during acceleration and deceleration (see Figure 4). When the rotor is
properly installed, the pins sit either in the drive hub grooves or on top of the hub surface (Figure 5).
The rotor pins are positioned parallel to the Beckman Coulter name engraved at the center of the
rotor body (see Figure 6). The markings on the lifting ring, located near the top periphery of the
rotor, also are indicators of the drive pin position. Knowing the pin orientation before you install
the rotor will help to ensure that you position the rotor properly on the hub, minimizing the chance
of hub damage. Follow the steps below.
6
PN B32164AC
Page 17
Figure 4 Rotor Drive Pin Location and Orientation
1
6
4
3
2
7
8
5
9
JA-14.50 Fixed-Angle Rotor
Preparation and Use
1. Rotor Drive Hole
2. Drive Pins
Figure 5 Centrifuge Drive Hub Configurations
1. Drive Hub Grooves (4)
2. Rotor Pins (2)
3. Drive Hub
4. Drive Spindle Assembly
5. Older Model Centrifuges
Be sure the pins in the rotor drive hole
are seated in the grooves of the drive
spindle hub as shown
3. Avanti J Centrifuge
Drive Spindle Hub
6. Te e th ( 2)
7. Drive Hub
8. Drive Spindle Assembly
9. Newer Model Centrifuges
Be sure the pins in the rotor drive
hole are not sitting on top of the
teeth on the drive spindle hub
PN B32164AC
7
Page 18
JA-14.50 Fixed-Angle Rotor
1
4
3
2
Preparation and Use
Figure 6 Installing the Rotor
1. To p Vi e w 2. Side View
Rotor Lid Configurations
The rotor has two available lid configurations.
• The dual-locking lid (B19947) allows the lid to be attached to and removed from the rotor
outside the centrifuge, so that tubes can be loaded and unloaded under a safety hood. The duallocking lid consists of a daisy knob that attaches the lid to the rotor body, and a tie-down knob
that attaches the rotor to the centrifuge drive hub.
1. Tie-down Knob
2. Daisy Knob
3. Lubricate Threads
4. Dual-Locking Lid
8
PN B32164AC
Page 19
JA-14.50 Fixed-Angle Rotor
1
3
2
Preparation and Use
• The single-locking lid (B19952) has a tie-down knob that secures the rotor to the centrifuge
drive spindle. The rotor must be placed in the centrifuge before the lid is attached.
1. Tie-down Knob
2. Lubricate Threads
3. Single-Locking Lid
Rotor Installation
1
To prevent the rotor from sticking to the centrifuge drive hub, apply a thin coat of Spinkote
lubricant to the rotor drive pins and to the lid knob threads.
2
Lightly coat the large and small O-rings with silicone vacuum grease.
3
Dual-locking lid only. Place filled tubes into the rotor, then place the lid on the rotor and turn the
daisy knob to the right (clockwise) until secure.
Do not overtighten.
4
Turn the rotor until the Beckman Coulter name or the engraved drive pin alignment indicators
are either horizontal or perpendicular to the drive hub pins or teeth (see Figure 4).
Or, rotate the drive spindle to align the hub pins or teeth with the rotor drive pins.
5
Carefully lower the rotor straight down onto the centrifuge drive spindle hub.
Do not drop the rotor onto the spindle hub.
6
Slowly turn the rotor around the drive spindle to make sure that it is properly seated.
In rare cases, the rotor pins can rest on top of the spindle hub pins or teeth, which prevents the
rotor from being secured to the drive spindle.
Turning the rotor after installing it will move the pins into proper position.
PN B32164AC
9
Page 20
JA-14.50 Fixed-Angle Rotor
Preparation and Use
7
Secure the rotor to the centrifuge drive spindle as follows:
a. Dual-locking lid: press the lid knob down and turn it to the right (clockwise) until secure,
no more than two full turns.
Do not overtighten.
b. Single-locking lid: place filled tubes into the rotor, then place the lid on the rotor.
Press the lid knob down and turn it to the right (clockwise) until secure.
Do not overtighten.
CAUTION
If the rotor is left in the centrifuge between runs, make sure that the rotor is
seated on the drive hub and that the tie-down knob is tight before each run.
Run Procedure
When the rotor is properly loaded and installed in the centrifuge, you are ready to perform the run.
Refer to the applicable centrifuge instruction manual for operating instructions.
WARNING
Operator error or tube failure may generate aerosols. Toxic, pathogenic, or other
hazardous materials must not be run in this rotor unless all appropriate safety
precautions are taken. Also see the Safety Notice at the beginning of this manual.
CAUTION
Make sure that the rotor is properly seated on and securely fastened to the drive
hub before each run.
Removal and Sample Recovery
CAUTION
If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply all appropriate safety and decontamination procedures
to the centrifuge and accessories.
10
Dual-Locking Lid
1
Place one hand on the rotor to keep it from turning.
PN B32164AC
Page 21
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Grasp the round lid knob with your other hand and turn the knob to the left (counterclockwise)
until the stem disengages from the drive hub threads.
2
Hold the knob or place your hands on the rotor and lift the rotor straight up off the drive hub.
3
Place the rotor on a counter or bench top under a safety hood.
4
Grasp the daisy knob and turn it to the left (counterclockwise) until it disengages.
5
Remove the lid and set it aside.
Single-Locking Lid
1
Unscrew the lid knob.
2
Remove the lid and set it aside.
PN B32164AC
3
Lift the rotor straight up off the drive hub to avoid bending or damaging the hub.
11
Page 22
JA-14.50 Fixed-Angle Rotor
Preparation and Use
If the Rotor Sticks to the Drive Hub
If the rotor sticks to the centrifuge drive hub, use the removal tool (360998) to release it as follows
(see Figure 7.
Figure 7 Using the Rotor Removal Tool
1. Rotor Removal Tool (360998)
NOTE
1
2
3
4
This procedure requires you to remove the rotor lid while the rotor is in the centrifuge. If you are using
the dual-locking lid, the biosafe seal provided by this mechanism will be broken when the lid is removed.
Consult your laboratory safety officer for the proper safety procedures to follow in this case.
Remove the lid.
Screw the removal tool into the threaded opening.
As the tool tightens down it will push against the drive hub, forcing the rotor up and off the hub.
Lift the rotor up and out of the centrifuge by the removal tool handle or by holding the rotor
with your hands.
Unscrew the removal tool and remove it from the rotor.
Never run the rotor with the removal tool in place.
NOTE
12
Lubricate the centrifuge drive hub with a light coat of Spinkote to prevent further sticking.
PN B32164AC
Page 23
Tub es
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Tubes for JA-14.50 rotors manufactured prior to October 2016 are listed in Table 1 and Table 2. Tubes
for JA-14.50 rotors manufactured after October 2016 are listed in Table 3 and Table 4. Be sure to use
only those items listed, and to observe the maximum speed limits and fill volumes shown.
(Maximum fill volume is the maximum amount that can be centrifuged in the container listed.)
NOTE
JA-14.50 rotors and lids manufactured after October 2016 include the letter “T” after the serial number
(Fig ure 8 and Fig ure 9).
Figure 8 Rotor Serial Number with T Figure 9 Rotor Lid Serial Number with T
1.T– designates the rotor and lid were manufactured after October 2016
Refer to Chemical Resistances (publication IN-175) for information on the chemical compatibilities of
tube and accessory materials.
Tubes for JA-14.50 Rotors Manufactured before October 2016
CAUTION
The tubes listed in Table 1 should be used one time only. Tube damage and sample
leakage will result from repeated use.
Do not use glass tubes in this rotor.
Tab l e 1 Adapters to support tubes from other manufacturers for the JA-14.50 Rotora manufactured before October 2016
Type a n d
Volume
conical
50 mL
conical
15 mL
PN B32164AC
Tube Description Required Adapter
Polypropylene conical tube
with cap
Polypropylene conical tube
with cap
bcdef
bcef
Effective
r
max
(mm)
N/A 159.7 98.8
B40156
(Qty 2 ea. B30033)
156.2 92.2
Effective
r
min
(mm)
Max
Speed/RCF/
k Factor
g
g
13
Page 24
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Tab l e 1 Adapters to support tubes from other manufacturers for the JA-14.50 Rotora manufactured before October 2016
Type a n d
Volume
conical
5 mL
conical
Tube Description Required Adapter
Polypropylene conical tube
with tethered cap
Spin filtration device
h
ij
Effective
r
max
(mm)
B40158
(Qty 2 ea. B30035)
125.1 95.7
N/A 159.7 98.8
Effective
r
min
(mm)
Max
Speed/RCF/
k Factor
g
g
50 mL
conical
Spin filtration device
15 mL
a. Use only the items listed here and observe fill volumes and maximum run speeds.
b. Manufactured by Greiner.
c. Manufactured by BD Falcon.
d. Manufactured by VWR.
e. Manufactured by Corning.
f. Manufactured by NUNC.
g. Use manufactures' recommendations for tube and bottle RCF limits. The corresponding RPM and k factor can be calculated using the equations
on pages 1 and 15, respectively.
h. Manufactured by Eppendorf.
i. Manufactured by Pall.
j. Manufactured by Millipore.
ij
B40156
(Qty 2 ea. B30033)
156.2 92.2
g
Tab l e 2 Available Beckman Coulter Bottles and Tubes for the JA-14.50 Rotora manufactured prior to October 2016
Nominal
Dimensions
and Volume
29 104 mm
50 mL
29 104 mm
50 mL
29 104 mm
50 mL
29 104 mm
50 mL
29 104 mm
50 mL
Tube Description Part
Number
Polycarbonate Round Bottom
361693 40 B40155
bottles with cap assemblies
Polypropylene Round Bottom
361694 40 B40155
bottles with cap assemblies
Polycarbonate Round Bottom
357002 40 B40155
bottles with screw caps
Polypropylene Round Bottom
357003 40 B40155
bottles with screw caps
Polycarbonate Round Bottom
363664 36.5 B40155
tubes with snap-on caps
Nominal
Volume
(mL)
Required
Adapter
P/N
(Qty 2 ea
B30032)
(Qty 2 ea
B30032)
(Qty 2 ea
B30032)
(Qty 2 ea
B30032)
(Qty 2 ea
B30032)
Tube s
per
Adapter
Max
Speed/RCF/
k Factor
1 14,000 RPM
34,520 g
563
1 14,000 RPM
34,520 g
563
1 14,000 RPM
34,520 g
563
1 14,000 RPM
34,520 g
563
1 14,000 RPM
34,520 g
563
14
PN B32164AC
Page 25
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Tab l e 2 Available Beckman Coulter Bottles and Tubes for the JA-14.50 Rotora manufactured prior to October
Nominal
Dimensions
and Volume
29 104 mm
50 mL
29 104 mm
50 mL
29 104 mm
50 mL
18 100 mm
15 mL
18 100 mm
15 mL
18 100 mm
15 mL
Tube Description Part
Number
Polypropylene Round Bottom
357005 36.5 B40155
tubes with snap-on caps
Polycarbonate Round Bottom
363647 35 B40155
open-top tubes
Polypropylene Round Bottom
357007 35 B40155
open-top tubes
Polycarbonate Round Bottom
342080 15 B40157
open-top tube
Polypropylene Round Bottom
342081 15 B40157
open-top tube
Polyethylene Round Bottom
342082 15 B40157
open-top tube
Nominal
Volume
(mL)
Required
Adapter
P/N
(Qty 2 ea
B30032)
(Qty 2 ea
B30032)
(Qty 2 ea
B30032)
(Qty 2 ea
B30034)
(Qty 2 ea
B30034)
(Qty 2 ea
B30034)
Tube s
per
Adapter
Max
Speed/RCF/
k Factor
1 14,000 RPM
34,520 g
563
1 14,000 RPM
34,520 g
563
1 14,000 RPM
34,520 g
563
1 14,000 RPM
33,400 g
665
1 14,000 RPM
33,400 g
665
1 14,00 RPM
33,400 g
665
16 80 mm
10 mL
11 38 mm
Polycarbonate Round Bottom
355672 10 361703 &
bottle with cap assembly
Polypropylene microfuge tube 357448 1.5 361705
1.5 mL
11 38 mm
Polypropylene microfuge tube 356090 1.5 361705 &
1.5 mL
a. Use only the items listed here and observe fill volumes and maximum run speeds.
B40155
(Qty 2 ea
B30032)
&B40155
(Qty 2 ea
B30032)
B40155
(Qty 2 ea
B30032)
1 14,000 RPM
30,900 g
445
3 14,000 RPM
27,000 g
449
3 14,000 RPM
27,000 g
449
PN B32164AC
15
Page 26
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Tubes for JA-14.50 Rotors Manufactured after October 2016
NOTE
JA-14.50 rotors and lids manufactured after October 2016 include the letter “T” after the serial number
(see Figure 8 and Figu re 9).
CAUTION
The tubes listed in Table 3 should be used one time only. Tube damage and sample
leakage will result from repeated use.
Do not use glass tubes in this rotor.
Tab l e 3 Adapters to support tubes from other manufacturers for the JA-14.50 Rotora manufactured after October 2016
Type and
Volume
conical
50 mL
conical
15 mL
conical
5 mL
Tube Description Required
Adapter
Polypropylene conical tube with cap
Polypropylene conical tube with cap
bcdef
bcef
N/A 159.7 98.8
B89429
(Qty 2 ea
B19962)
Polypropylene conical tube with tethered
h
cap
B89431
(Qty 2 ea
B19964)
Effective
r
max
Effective
(mm)
156.2 92.2
125.1 95.7
r
min
(mm)
Max
Speed/RCF/
k Factor
g
g
g
conical
Spin filtration device
ij
N/A 159.7 98.8
g
50 mL
conical
15 mL
Spin filtration device
ij
B89429
156.2 92.2
(Qty 2 ea.
g
B19962)
a. Use only the items listed here and observe fill volumes and maximum run speeds.
b. Manufactured by Greiner.
c. Manufactured by BD Falcon.
d. Manufactured by VWR.
e. Manufactured by Corning.
f. Manufactured by NUNC.
g. Use manufactures' recommendations for tube and bottle RCF limits. The corresponding RPM and k factor can be calculated using the equations
on pages 1 and 15, respectively.
h. Manufactured by Eppendorf.
i. Manufactured by Pall.
j. Manufactured by Millipore.
16
PN B32164AC
Page 27
JA-14.50 Fixed-Angle Rotor
2°C
Preparation and Use
Tab l e 4 Available Beckman Coulter Bottles and Tubes for the JA-14.50 Rotora manufactured after October 2016
Nominal
Dimensions
and Volume
18 100 mm
15 mL
18 100 mm
15 mL
18 100 mm
15 mL
a. Use only the items listed here and observe fill volumes and maximum run speeds.
Tube Description
Polycarbonate Round Bottom
open-top tube
Polypropylene Round Bottom
open-top tube
Polyethylene Round Bottom
open-top tube
Part
Number
342080 15 B89430
342081 15 B89430
342082 15 B89430
Temperature Limits
• Plastic tubes have been centrifuge tested for use at temperatures between 2 and 25°C. For
centrifugation at other temperatures, pretest tubes under anticipated run conditions.
• If plastic containers are frozen before use, make sure that they are thawed to at least 2°C prior
to centrifugation.
Nominal
Volume
(mL)
Required
Adapter
P/N
(Qty 2 ea
B19963)
(Qty 2 ea
B19963)
(Qty 2 ea
B19963)
Tube s
per
Adapter
1 14,000 RPM
1 14,000 RPM
1 14,000 RPM
Speed/RCF/
k Factor
33,400 g
33,400 g
33,400 g
Max
665
665
665
The JA-14.50 rotor generates g-forces higher than the tube manufacturers’ recommended
maximums (see Table 1, Table 2, Table 3, and Table 4). Running tubes above the recommended
maximum g-forces will cause white striations known as crazing (see Figure 10). Moderate crazing
will not affect run results or cause tube leakage. However, repeated tube usage and/or long run
times will eventually lead to tube failure. To minimize the possibility of tube failure, observe the
following conditions.
• Do not use tubes more than once.
• Discard crazed tubes.
• Refer to Table 5 to find the g-forces produced at various speeds in the rotor, and limit run speed
to the speed that produces the maximum recommended g-force for the tube in use.
PN B32164AC
17
Page 28
JA-14.50 Fixed-Angle Rotor
k
r
maxrmin
⁄()ln
ω
2
-----------------------------------
10
13
3600
------------
×=
k
2.533 10
11
×()r
max
r⁄
min
()ln
RPM()
2
-------------------------------------------- -------------------------- --
=
t
k
s
---
=
t
787
3600
------------
13 min≈=
Preparation and Use
Figure 10 Examples of Tubes with Minimum and Moderate Crazing
Run Times
The k factor of the rotor is a measure of the rotor’s pelleting efficiency. Beckman Coulter has
calculated the k factors for all of its rotors, at maximum speed with full tubes, using the following
formula:
EQ 1
where ω is the angular velocity of the rotor in radians per second ( = 0.105 RPM), r
maximum radius, and r
is the minimum radius.
min
max
is the
After substitution:
EQ 2
In the JA-14.50 rotor, the k factor is 787 for full 50-mL tubes at maximum speed.* For containers that
nominally hold less than 50 mL, the k factors will be different. Calculate the k factors for
these containers using EQ 2.
Use the k factor in the following equation to estimate the run time t (in hours) required to pellet
particles of known sedimentation coefficient s (in Svedberg units, S).
EQ 3
18
For example, at 14,000 RPM E. coli (s = 3600 S) will pellet in
EQ 4
* Based on sedimentation in water at 20°C.
PN B32164AC
Page 29
JA-14.50 Fixed-Angle Rotor
k
adjkrated
14, 000
actual run speed
-----------------------------------------
2
=
t
a
t
b
-----
k
a
k
b
------
=
Preparation and Use
Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor
as follows:
EQ 5
Run times can also be estimated from data established in prior experiments using a different rotor
if the k factor of the previous rotor is known. For any two rotors, a and b,
EQ 6
where the k factors have been adjusted for the actual run speed used.
PN B32164AC
19
Page 30
JA-14.50 Fixed-Angle Rotor
reduced maximum speed = (14,000 RPM)
1.2 g/mL
density of tube contents
------------------------------------------- ---------------
Preparation and Use
Run Speeds
The centrifugal force at a given radius in a rotor is a function of run speed. Comparisons of forces
between different rotors are made by comparing the rotors’ relative centrifugal fields (RCF). When
rotational speed is selected so that identical samples are subjected to the same RCF in two different
rotors, the samples are subjected to the same force (see Table 5).
Do not select rotational speeds higher than the maximums you have determined to be appropriate
for your protocols (in no case above 14,000 RPM). When solutions more dense than 1.2 g/mL are
centrifuged in this rotor, use EQ 7 to calculate the reduced maximum allowable rotor speed.
EQ 7
Tab l e 5 Relative Centrifugal Fields for the JA-14.50 Rotor
a
Rotor Speed (RPM) Relative Centrifugal Field (g)
At r
(160 mm) At rav (124 mm) At r
max
14,000
13,000
12,000
11,000
10,000
9000
8000
7000
6000
5000
4000
3000
2000
0
a. Entries in this table are calculated from the formula RCF = 1.12r (RPM/1000)
35,000
30,300
25,800
21,700
17,900
14,500
11,500
8800
6500
4500
2900
1600
700
0
27,200
23,500
20,000
16,800
13,900
11,200
8900
6800
5000
3500
2200
1300
600
0
2
and then rounded to three significant digits.
min
(87 mm)
19,000
16,500
14,000
11,800
9700
7900
6200
4800
3500
2400
1600
900
400
0
20
PN B32164AC
Page 31
Figure 11 Relative Centrifugal Fields Chart for the JA-14.50 Rotor
JA-14.50 Fixed-Angle Rotor
Care and Maintenance
Care and Maintenance
Maintenance
NOTE
1
Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins
in scratches and may open fissures in the rotor with continued use.
Periodically (at least monthly) inspect the rotor, especially inside cavities, for rough spots or
pitting, white powder deposits (frequently aluminum oxide), or heavy discoloration.
1. Daisy Knob
2. Small O-ring (961696)
3. Rotor Body
4. Check for Corrosion
5. Large O-ring (885501)
6. Dual Locking Lid (B19947)
PN B32164AC
If any of these signs are evident, do not run the rotor.
21
Page 32
JA-14.50 Fixed-Angle Rotor
Care and Maintenance
Contact your Beckman Coulter representative for information about the Field Rotor Inspection
Program and the rotor repair center.
2
Before using the rotor, inspect the rotor drive pins to ensure that they are not damaged.
Damaged drive pins can prevent the rotor from seating properly on the centrifuge drive hub
(Avanti J series centrifuges only).
To inspect the drive pins, turn the rotor upside down and look into the drive hole in the center
of the rotor.
3
Regularly lubricate the metal threads in the rotor with a thin, even coat of Spinkote lubricant.
Failure to keep these threads lubricated can result in damaged threads.
If the drive pins appear damaged, contact Beckman Coulter Field Service.
4
Regularly apply silicone vacuum grease to the O-rings.
*
Cleaning
Replace O-rings about twice a year or whenever worn or damaged.
5
Refer to Chemical Resistances (publication IN-175) for the chemical compatibilities of rotor and
accessory materials.
Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program
and the rotor repair center.
Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if
spillage has occurred. Do not allow corrosive materials to dry on the rotor.
Under normal use, wash the rotor frequently (at least weekly) to prevent buildup of residues.
1
Remove the O-rings before washing.
2
Wash the rotor and lid in a mild detergent, such as Solution 555 (339555), that won’t damage
the rotor.
22
Dilute the detergent with water (10 parts water to 1 part detergent).
* Call 1-800-742-2345 (U.S.A. or Canada); outside the U.S, contact your local Beckman Coulter office or visit us on the
web at www.beckmancoulter.com.
PN B32164AC
Page 33
JA-14.50 Fixed-Angle Rotor
Care and Maintenance
The Rotor Cleaning Kit (339558) contains two plastic-coated brushes and two quarts of
Solution 555 for use with rotors and accessories.
NOTE
3
Thoroughly rinse the cleaned rotor and components with distilled water.
4
Air-dry the rotor and lid upside down.
Do not use acetone to dry the rotor.
5
Apply a thin, even coat of silicone vacuum grease to both lid O-rings before replacing them
in the lid.
Do not wash rotor components in a dishwasher. Do not soak in detergent solution for long periods,
such as overnight.
1. Small O-ring
2. Large O-ring
6
Clean metal threads every 6 months, or as necessary.
Use a brush and concentrated Solution 555.
Rinse and dry thoroughly, then lubricate lightly but evenly with Spinkote to coat all threads.
7
Periodically remove the O-rings and wipe clean as necessary.
a. Clean the O-ring grooves with a cotton-tipped swab.
b. Reapply a light film of silicone vacuum grease.
Decontamination
PN B32164AC
23
Page 34
JA-14.50 Fixed-Angle Rotor
Care and Maintenance
If the rotor (and/or accessories) becomes contaminated with radioactive material, it should be
decontaminated using a solution that will not damage the anodized surfaces. Beckman Coulter has
tested a number of solutions and found two that do not harm anodized aluminum: RadCon Surface
Spray or IsoClean Solution (for soaking),
*
and Radiacwash.
†
NOTE
IsoClean can cause fading of colored anodized surfaces. Use it only when necessary and remove it
promptly from surfaces.
While Beckman Coulter has tested these methods and found that they do not damage components,
no guarantee of decontamination is expressed or implied. Consult your laboratory safety officer
regarding the proper decontamination methods to use.
If the rotor or other components are contaminated with toxic or pathogenic materials, follow all
appropriate safety and decontamination procedures as outlined by your laboratory safety officer.
Sterilization and Disinfection
• The rotor and all rotor components can be autoclaved at 121°C for up to an hour. Remove the
lid from the rotor and place the rotor and lid in the autoclave upside down.
• Ethanol (70%) or hydrogen peroxide (6%) may be used on all rotor components, including those
made of plastic. Bleach (sodium hypochlorite) may be used, but may cause discoloration of
anodized surfaces. Use the minimum immersion time for each solution, per laboratory
standards.
CAUTION
Ethanol is a flammability hazard. Do not use it in or near operating centrifuges.
Storage
While Beckman Coulter has tested these methods and found that they do not damage the rotor or
components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or
disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.
Refer to publication IN-192 (included with each box of tubes) for tube sterilization and disinfection
procedures.
When it is not in use, store the rotor in a dry environment (not in the instrument) with the lid
removed to allow air circulation so moisture will not collect in the tube cavities.
* In U.S., contact Nuclear Associates (New York); in Eastern Europe and Commonweath States, contact Victoreen GmbH
(Munich); in South Pacific, contact Gammasonics Pty. Ltd. (Australia); in Japan, contact Toyo Medic Co. Ltd. (Tokyo).
† In U.S., contact Biodex Medical Systems (Shirley, New York); internationally, contact the U.S. office to find the dealer
closest to you.
24
PN B32164AC
Page 35
Returning a Rotor
Before returning a rotor or accessory for any reason, prior permission must be obtained from
Beckman Coulter, Inc. A return authorization form may be obtained from your local
Beckman Coulter sales office. The form should contain the following information:
• rotor type and serial number
• history of use (approximate frequency of use)
• reason for the return
• original purchase order number, billing number, and shipping number, if possible
• name and email address of the person to be notified upon receipt of the rotor or accessory at
the factory
• name and email address of the person to be notified about repair costs, etc.
To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from
pathogens and/or radioactivity. Sterilization and decontamination must be done before returning
the parts. Smaller items (such as tubes, bottles, etc.) should be enclosed in a sealed plastic bag.
JA-14.50 Fixed-Angle Rotor
Returning a Rotor
All parts must be accompanied by a note, plainly visible on the outside of the box or bag, stating that they
are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach
this notification will result in return or disposal of the items without review of the reported problem.
Use the address label printed on the return form when mailing the rotor and/or accessories.
Customers located outside the United States should contact their local Beckman Coulter office.
Supply List
See the High Performance, High Speed, High Capacity Rotors, Tubes & Accessories catalog (BR-8102)
available at www.beckmancoulter.com for detailed information on ordering parts and supplies; or
call Beckman Coulter Customer Service at 1-800-742-2345 (U.S.A. or Canada). For your convenience,
a partial list is given below.
Replacement Rotor Parts
JA-14.50 rotor assembly, biosafe (with dual-locking lid) B88554
Description Part Number
PN B32164AC
JA-14.50 rotor assembly (with single-locking lid) B88555
Dual-locking lid B19947
Single-locking lid B19952
Lid (large) O-ring (Buna-N rubber) 885501
Rotor body (small) O-ring (ethylene propylene rubber) 961696
25
Page 36
JA-14.50 Fixed-Angle Rotor
Supply List
Supplies
NOTE
For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.
Description Part Number
Tubes and adapters see Ta b l e 1 , Ta b l e 2 , Ta b l e 3 , and Ta b l e 4
Rotor removal tool 360998
Rotor Cleaning Kit 339558
Silicone vacuum grease (1 oz) 335148
Solution 555 (1 qt) 339555
Spinkote lubricant (2 oz) 306812
26
PN B32164AC
Page 37
Beckman Coulter, Inc.
7
J Series Rotor Warranty
Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc.,
terms and conditions of sale in effect at the time of sale, Beckman Coulter, Inc. agrees to correct
either by repair, or, at its election, by replacement, any defects of material or workmanship
which develop within seven (7) years after delivery of a J series rotor to the original buyer by
Beckman Coulter, Inc. or by an authorized representative, provided that investigation and factory
inspection by Beckman Coulter, Inc. discloses that such defect developed under normal and proper
use. Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this
warranty, Beckman Coulter will supply free of charge all centrifuge parts required for repair.
Replacement
Any product claimed to be defective must, if requested by Beckman Coulter, Inc., be returned to the
factory, transportation charges prepaid, and will be returned to Buyer with the transportation
charges collect unless the product is found to be defective, in which case Beckman Coulter, Inc. will
pay all transportation charges.
A defective rotor will be replaced by Beckman Coulter, Inc. at its then current list price less a credit
based upon the age of the rotor (years since date of purchase). The Buyer shall not receive credit
until the claimed defective rotor is returned to Beckman Coulter’s Indianapolis, Indiana facility or
delivered to a Beckman Field Service representative.
The replacement price (cost to Buyer) for the respective rotor shall be calculated as follows:
Replacement price = Current rotor list price
Conditions
1. Except as otherwise specifically provided herein, this warranty covers the rotor only and
Beckman Coulter, Inc. shall not be liable for damage to accessories or ancillary supplies
including but not limited to (i) tubes, (ii) tube caps, (iii) tube adapters, or (iv) tube contents.
2. This warranty is void if the rotor has been subjected to customer misuse such as operation or
maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual.
3. This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge
unmatched to the rotor characteristics, or is operated in a Beckman Coulter centrifuge that has
been improperly disassembled, repaired, or modified.
Disclaimer
IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF
FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER, INC.
SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND
WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR,
MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.
PN B32164AC
Warranty-1
Page 38
Beckman Coulter, Inc. J Series Rotor Warranty
Warranty-2
PN B32164AC
Page 39
Page 40
www.beckmancoulter.com
Related Documents
Rotors and Tubes for Beckman Coulter J2,
J6,and Avanti J Series Centrifuges
PN JR-IM-10
• Rotors
• Tubes, Bottles, and Accessories
• Using Tubes and Accessories
• Using Fixed-Angle Rotors
• Using Swinging-Bucket Rotors
• Using Vertical-Tube and Rack Type Rotors
• Care and Maintenance
• Chemical Resistances
• Temperature Compensation Tables
• Gradient Materials
• Blood Component Separation
Available in electronic pdf or CD-ROM by request.
Rotors and Tubes CD-ROM (369668)
• Rotors and Tubes for Tabletop Preparative
Ultracentrifuges
• Rotors and Tubes for J2, J6, Avanti J Series
Centrifuges
• Rotors and Tubes for Preparative
Ultracentrifuges
• Rotor Safety Bulletin
• Chemical Resistances for Beckman Coulter
Centrifugation Products
Included with shipment of instrument.
Additional References
• Chemical Resistances for Beckman Coulter
Centrifugation Products (IN-175)
• Beckman Coulter High Performance, High
Speed, High Capacity Rotors, Tubes &
accessories catalog (BR-8102)
• Use and Care of Centrifuge Tubes and Bottles
(IN-192)
Available in hard copy or electronic pdf by
request.
© 2016 Beckman Coulter, Inc.
All Rights Reserved
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