Page 1
Instruction For Use
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
GH-3.8 Swinging Bucket Rotor
In Beckman Coulter Allegra 6 Series, GS-6 Series, Spinchron Series, and GP Series Centrifuges
PN GS6-TB-003MD
June 2018
Page 2
GH-3.8 Swinging Bucket Rotor
Beckman Coulter Eurocenter S.A.
22, rue Juste-Olivier
Case Postale 1044
CH - 1260 Nyon 1, Switzerland
Tel: +41 (0) 22 365 36 11
In Beckman Coulter Allegra 6 Series, GS-6 Series,
Spinchron Series, and GP Series Centrifuges
PN GS6-TB-003MD (June 2018)
© 2018 Beckman Coulter, Inc.
All rights reserved.
Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Contact Us
If you have any questions, contact our Customer Support
Center.
• Worldwide, find us via our website at
www.beckman.com/support/technical
• In the USA and Canada, call us at 1-800-369-0333.
• Outside of the USA and Canada, contact your local
Beckman Coulter Representative.
Find us on the World Wide Web at:
www.beckman.com
Original Instructions
Page 3
This safety notice summarizes information basic to the safe use of the equipment described in this
publication. The international symbol displayed above is a reminder to the user that all safety
instructions should be read and understood before operation or maintenance of this equipment is
attempted. When you see the symbol on other pages of this publication, pay special attention to the
specific safety information presented. Observance of safety precautions will also help to avoid
actions that could damage or adversely affect the performance of the rotor system.
Safety Notice
• This rotor is warranted for 7 years (see the warranty at the back of this publication). Beckman
Coulter recommends that you retire the rotor from use at the end of the 7-year warranty period
to prevent the possibility of a rotor mishap resulting from material fatigue.
• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and C)
and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi— further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this rotor without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.
Safety Notice
• Dispose of all waste solutions according to appropriate environmental health and safety
• The rotor and accessories are not designed for use with materials capable of developing
• If glass tubes break, remove the glass very carefully from the adapter, bucket, or cavity. If all
• Inspect the rotor once a month, especially inside cavities, for rough spots or pitting, white
PN GS6-TB-003MD
guidelines.
flammable or explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl
alcohol) in or handle or store them near the centrifuge.
the glass particles are not contained in the bucket or cavity, be careful when examining or
cleaning the centrifuge gasket and chamber as glass particles may be embedded in their
surfaces.
powder deposits—frequently aluminum oxide—or heavy discoloration. If any of these signs are
evident, do not run the rotor. Contact your Beckman Coulter representative for information
bout the Field Rotor Inspection Program and the rotor repair center. To reduce the potential
for corrosion, clean buckets or carriers thoroughly immediately following a tube or well plate
breakage. Be sure to remove all glass particles from buckets or carriers.
iii
Page 4
Safety Notice
Safety Notice
• Components or accessories designed for other rotors may cause rotor mishap if used in this
rotor. Use only components and accessories that have been designed for use in this rotor. The
safety of rotor components and accessories made by other manufacturers cannot be ascertained by
Beckman Coulter. Use of other manufacturers’ components or accessories in the rotors may void the
rotor warranty and should be prohibited by your laboratory safety officer. If tubes, microplates, or
other labware made by manufacturers other than Beckman Coulter are used, reduce rotor
speed to prevent breakage. The strength of glass and plastic tubes can vary between lots, and
will depend on handling and usage; we highly recommend that you pretest labware in the rotor
using water samples to determine optimal operating conditions. Scratches (even microscopic
ones) significantly weaken glass tubes.
• Do not substitute a metal fastener for the plastic tiedown nut supplied with the rotor. If a tie
down nut comes off the shaft during centrifugation, the plastic nut furnished will break apart
in the chamber, causing minimal damage to the instrument. A loose metal object could
substantially damage the rotor, chamber, and lid, and could potentially escape the chamber
into the laboratory, causing personal injury or property damage.
• The rotor must be run with a full complement of buckets and/or multiwell plate carriers
attached to the yoke. You can run four buckets, four multiwell plate carriers, or two buckets
and two carriers (with like components loaded opposite each other). If only two buckets are
loaded with blood bags, bottles, or modular disk adapters, the other two buckets should contain
at least a minimal “blank” load (e.g., empty modular disk adapters) to achieve optimal results
and to avoid rotor imbalance.
• The maximum allowable run speed (3750 rpm) listed in the rotor specifications is for operation
when all conditions are within the standard specifications (using buckets). Maximum allowable
run speed when using Micro Plus multiwell plate carriers is 3250 rpm. Do not overload the rotor
without reducing the speed (see SPEED DERATING). Failure to derate will reduce the safe useful
life of the rotor.
• If disassembly reveals evidence of leakage, you should assume that some fluid escaped the
rotor. Apply appropriate decontamination procedures should be applied to the centrifuge and
accessories.
iv
PN GS6-TB-003MD
Page 5
Alerts for Danger, Warning, Caution, Important, and Note
Alerts for Danger, Warning, Caution, Important, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury.
Safety Notice
IMPORTANT
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.
NOTE
or servicing of this equipment.
IMPORTANT is used for comments that add value to the step or procedure being performed.
NOTE is used to call attention to notable information that should be followed during installation, use,
PN GS6-TB-003MD
v
Page 6
Safety Notice
Alerts for Danger, Warning, Caution, Important, and Note
vi
PN GS6-TB-003MD
Page 7
Contents
Safety Notice,iii
Safety Notice,iii
Alerts for Danger, Warning, Caution, Important, and Note,v
GH-3.8 Rotor,1
Specifications,1
Description,3
The Rotor,3
Buckets and Accessories,3
Modular Disk Adapters,3
Bottle Adapters,4
Blood Bag Cups,4
Bucket Covers,4
Aerosolve Cannisters,4
Micro Plus Multiwell Plate Carriers,10
Installation,11
The Rotor Yoke,11
Buckets or Carriers,14
Loading the Rotor,15
Working with Physiological Fluids,15
Symmetric and Balanced Loading,15
Using Buckets,19
Assembling Modular Disk Adapters,20
Loading Tubes in Adapters,21
Loading Adapters in the Buckets,21
Using Bottles in Adapters,22
Using Blood Bag Cups,22
Bucket Covers,23
Using Aerosolve Cannisters,25
Using Micro Plus Multiwell Plate Carriers,26
Operation,27
Speed Derating,27
Temperature Limits,28
Run Procedure,28
Care and Maintenance,31
Inspection,31
Maintenance,31
Cleaning,32
The Rotor Components,32
vii
Page 8
Contents
Buckets,33
Micro Plus Plate Carriers (Including Base and Pad), Blood Bag
Cups, and Bucket Covers,33
Modular Disk Adapters,33
Aerosolve Cannisters,34
Decontamination,34
Sterilization and Disinfection,34
Tube Breakage,35
Aerosolve Cannisters,35
SST or Corvac Tubes,35
Returning a Rotor,36
Supply List,37
Replacement Rotor Parts,37
Supplies,38
Benchtop Rotor Warranty
Conditions,1
Disclaimer,1
viii
Page 9
Illustrations
Illustrations
1 The Micro Plus Multiwell Plate Carrier,10
2 Rotor Yoke and Centrifuge Drive Shaft. Be sure the pin in the
drive shaft is in the groove in the rotor yoke.,13
3 Rotor Tie-Down Nut and O-Ring,14
4 Examples of Correctly and Incorrectly Loaded Buckets and
Carriers. Contents of opposing buckets must be the same and
each bucket must be balanced on its pivotal axis.,16
5 The Bails (Vertical Supports) of the GH-3.8 (and GH-3.7) Modular
Disk Adapters and the J6 Series Adapters are Not the Same.
Bails for the GH-3.8 are straight and end in a disk; bails for
the J6 have curved ends. Do not intermix adapters (or disks
from adapters) between sets.,18
6 Examples of the Effects on the Horizontal
Swing of Buckets Caused by Proper and Improper
Weight Distribution,19
7 Assembling a Modular Disk Adapter,20
8 Installing an Adapter in a Bucket. Be sure the bails (vertical
supports)
line up with the bucket pivot pins.,21
9 Blood Bag Loading Procedures,23
10 Proper Placement of O-Ring,24
11 Securing Bucket Cover Latches,24
12 The Micro Plus Multiwell Plate Carrier, Base, Pad, and Deep-well
Plate,26
13 Lubricating Pivot Pin/Bucket Contact Areas,32
ix
Page 10
Tables
Tables
1 Relative Centrifugal Fields for the GH-3.8 Rotor. Entries in the
table are
calculated from the formula RCF = 1.12 r (RPM/1000)2and are
then rounded to
three significant digits.,2
2 Modular Disk Adapters, Bottle Adapters, and Cups Available for
the GH-3.8 Rotor,5
3 Beckman Coulter Tubes and Bottles for the GH-3.8 Rotor,7
4 Labware for Use with Micro Plus Carriers,12
5 Recommended g-Force Limits to Minimize Breakage of Certain
Glass Tubes and Bottles Made by Other Manufacturers in the
GH-3.8 Rotor,29
6 Approximate Acceleration and Deceleration Times for the GH-
3.8 Rotor in Allegra 6 Series,
GS-6 Series, and Spinchron Series Centrifuges,30
7 Approximate Acceleration and Deceleration Times for the GH-
3.8 Rotor in
GP Series Centrifuges,30
x
Page 11
This rotor has been manufactured in a registered ISO 9001 or 13485 facility for use with the specified
Beckman Coulter centrifuges.
Specifications
a
The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing through
the critical speed range is characterized by some vibration.
b
Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rω2) to the standard
acceleration of gravity (g) according to the following formula:
GH-3.8 Rotor
SPECIFICATIONS
Maximum speed (buckets). . . . . . . . . . . . . . . . . . . . . . . 3750 rpm
Maximum speed (Micro Plus carriers) . . . . . . . . . . . . . . 3250 rpm
Critical speed range
Maximum solution density . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL
Relative Centrifugal Fields
(see Table 1 for RCF at other speeds)
using buckets (rmax = 204 mm). . . . . . . . . . . . . . . . . 3210 × g
using Micro Plus carriers (rmax = 163 mm) . . . . . . . . 1924 × g
Conditions requiring speed reduction . . . . . . see Speed Derating
Number buckets or carriers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Available tubes and bottles . . . . . . . . . . . . . . . . . . . . . see Table 3
Maximum load allowed in each bucket at rated
speed (excluding weight of bucket and cover). . . 1000 grams
Maximum load allowed in each Micro Plus carrier
at rated speed (excluding weight of carrier). . . . . . 500 grams
Maximum rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . .3 liters
Approximate acceleration and
deceleration times . . . . . . . . . . . . . . . .see Table 6 and Table 7
Weight of fully loaded rotor
(buckets with covers). . . . . . . . . . . . . . . . . . . .11.4 kg (25.2 lb)
Rotor yoke material . . . . . . . . . . . . . . . . . . . . . . . . .stainless steel
Bucket material . . . . . . . . . . . . . . . . . . . . . . anodized aluminum
Carrier material . . . . . . . . . . . . . . . . . . . . . . anodized aluminum
a
. . . . . . . . . . . . . . . . . . . . . 400 to 1450 rpm
b
at maximum speed
PN GS6-TB-003MD
where r is the radius in millimeters, ω is the angular velocity in radians per second (2π RPM /60), and g is the standard acceleration
of gravity (9807 mm/s
2
). After substitution:
1-1
Page 12
GH-3.8 Rotor
Relative Centrifugal Field (RCF)
GH-3.8 Rotor
Speed (rpm)
0
500
1000
1500
2000
2500
3000
3500
0 2000 4000
Buckets
Micro Plus
RCF (× g)
30001000
Specifications
Table 1 Relative Centrifugal Fields for the GH-3.8 Rotor. Entries in the table are
2
calculated from the formula RCF = 1.12 r (RPM/1000)
and are then rounded to
three significant digits.
Rotor
Speed
(rpm)
3750
3500
3250
3000
2750
2500
2250
2000
1750
1500
1000
500
Relative Centrifugal Field (× g) at r
Buckets
(204 mm)
Micro Plus Carriers
(163 mm)
3210
2800
2400
2060
1730
1430
1160
913
700
514
228
57
Don’t run above 3250
1928
1643
1381
1141
924
730
559
411
183
46
max
1-2
PN GS6-TB-003MD
Page 13
Description
Specific information about the GH-3.8 rotor is given here. Information about the use and care of centrifuges
is contained in the individual instrument instruction manuals, which should be used in combination with
this manual for complete centrifuge and rotor operation information.
The Rotor
The GH-3.8 rotor, rated for 3750 rpm, is used in Beckman Coulter Allegra 6 series, GS-6 series,
Spinchron series, and GP series centrifuges. This four-place swinging bucket rotor carries a wide
range of tube and bottle sizes (from 1.5 to 750 mL) or single- to quad-pack blood bags in buckets, as
well as 96-well multiwell plates in specially designed carriers. This rotor rapidly sediments protein
precipitates, large particles, cells, and cell debris. It can also be used for binding studies and for
separating serum from whole blood.
The rotor yoke is made of stainless steel. Either buckets or multiwell plate carriers, both made of
anodized aluminum, may be run by placing them over pivot pins on the arms of the yoke. Both
buckets and carriers swing out to a horizontal position during centrifugation.
GH-3.8 Rotor
Description
CAUTION
Although rotor components and accessories made by other manufacturers may fit
in the GH-3.8 rotor, their safety in this rotor cannot be ascertained by Beckman
Coulter. Use of other manufacturers’ components or accessories in the
GH-3.8 rotor may void the rotor warranty and should be prohibited by your
laboratory safety officer. Only the components and accessories listed in this
publication should be used in this rotor.
Buckets and Accessories
Several types of labware can be placed in the buckets, depending upon your application: modular
disk adapters (for tubes of various sizes), bottle adapters, blood bag cups, and Aerosolve cannisters
(when aerosol containment is required). Bucket covers are also available as an aid toward
containment.
Modular Disk Adapters
Tubes are supported in modular disk adapters, which can also serve as tube racks in the laboratory.
The adapter disks are color coded by the tube size they accommodate (see Table 2); the number of
disks used in an adapter assembly depends upon the length of tubes used. A tube decanter is
available to hold either 10-mm or 12-mm tubes securely in the blue adapter, allowing all the tubes
to be decanted at once. Additionally, 1.5-mL Microfuge tubes can be run using a special plate that
fits on top of the blue adapter. Both of these accessories are described in Table 2. Beckman Coulter
tubes and bottles available for use in the GH-3.8 rotor buckets are described in Table 3.
PN GS6-TB-003MD
1-3
Page 14
GH-3.8 Rotor
Description
Bottle Adapters
Bottles are supported in polypropylene adapters that fit inside the rotor buckets. The adapters are
ribbed for strength and accommodate three bottle sizes, including one conical bottle (see Table 2).
Blood Bag Cups
Polypropylene cups provide support for blood bags in the rotor buckets. Blood bag cups are
available in two sizes: one for single- or double-pack bags, and one for triple- or quadpack bags (see
Table 2).
Bucket Covers
Transparent covers made of a high-impact plastic are available for the GH-3.8 rotor buckets. Each
cover requires an O-ring (made of ethylene propylene) that seats on a ledge inside the bucket. The
covers are held in place by attached latches. Although the covers are not designed to contain aerosols
that may result from tube breakage, they will contain most liquids and broken tube particles,
reducing the need to clean the centrifuge chamber, and allowing you to take appropriate
precautions before opening the covers in the event of tube breakage.
Aerosolve Cannisters
Aerosolve cannisters, designed to minimize aerosol leakage and liquid spills, can be used in the GH
3.8 buckets when this type of containment is required. The cannister was tested
containment of microbiological aerosols under normal operating conditions of the associated
Beckman Coulter centrifuge, when used and maintained as instructed. Aerosolve cannisters hold a
variety of tube sizes in racks, or they can be used as 500-mL wide-mouth bottles.
*
to demonstrate
1-4
* Validation of microbiological containment was done at an independent third-party testing facility (CAMR,
Porton Down, UK, or USAMRIID, Ft. Detrick, MD, U.S.A.). Improper use or maintenance may affect seal integrity and
thus containment.
PN GS6-TB-003MD
Page 15
Table 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the GH-3.8 Rotor
MODULAR DISK ADAPTERS (polypropylene)
GH-3.8 Rotor
Description
Nom.
tube
Color
Code
Vol.
(mL)
blue 3
5
tan 3 &
Max.
Nom.
Tube
Dia.
(mm)
10
No.
Tubes
per
Adapter
37 148 184.7 2910 5 359469 359148
12
13 30 120 184.7 2910 5 359478 359157
Max.
No.
tubes
in
Rotor
r
at
max
Adapter
Bottom
(mm)
RCF at
maximum
Speed
(x g)
No.
Disks
per
Adapter
Adapter Part No.
Set of
Two
Set of
Four
5
orange 7 &
14 24 96 184.7 2910 6 359470 359149
10
purple 12 16 19 76 184.7 2910 7 359471 359150
green
15 18 14 56 194.7 3070 6 359472 359151
(conical)
green 15 &
18 14 56 184.7 2910 7 359473 359152
20
lit. green
(conical)
30 &
50
30 4 16 191 3010 5 359475 359154
yellow 50 29 7 28 184.7 2910 6 359474 359153
dk. blue 50 35 4 16 184.7 2910 7 359476 359155
brown 100 44 2 8 184.7 2910 3 359477 359156
tube
decanter
1.5-mL
adapter
3
5
1.5 &
1.8
10
37 148 — — 1
12
11 26 104 — — 1
343108
(each)
354511
(each)
a
a
—
—
plate
BLOOD BAG CUPS (polypropylene)
r
max
at Cup
Bottom
(mm)
RCF at
Maximum
Speed
(x g)
Part
Number
(qty one)
Color
Code
Cup
Capacity
Size
(mm)
Number
Bags
per
Cup
yellow single bag double pack 90 2 196.2 3090 356856
orange triple pack quad pack 97 1 196.7 3100 356857
PN GS6-TB-003MD
1-5
Page 16
GH-3.8 Rotor
Description
Table 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the GH-3.8 Rotor (Continued)
AEROSOLVE CANNISTER RACKS (polypropylene)
Max.
r
Color
Code
Nom.
Tube
Vol.
(mL)
Nom.
Tube
Dia.
(mm)
No.
Tubes
per
Adapter
Max. No.
Tubes
in
Rotor
at
max
Adapter
Base
(mm)
RCF at
Maximum
Speed
b
(× g)
Set of
Adapter Part
Number
Two
Set of
Four
white 1.5 11 24 96 174 2740 354495
—
(each)
blue 3 & 5 12 24 96 174 2740 359482 359160
tan 5 12 24 96 180 2840 359489 358993
orange 10 14 18 72 175 2760 359483 359161
purple 12
3 & 5
white
15 14 10 40 174 2740 344517
(vials)
green 15 & 20
3 & 5
lt. green 15 (conical)
3 & 5
16
12
18
12
17
12
12
12
48
6
24
177
178
2790
2800
359484 359162
—
(each)
48
6
6
6
24
24
24
174
176
181
180
2740
2770
2850
2840
359485 359163
359487 358991
(round bottom)
lime
green
50 (conical)
3 & 5
30
12
4
4
16
16
181
180
2850
2840
359488 358992
(round bottom)
yellow 50
3 & 5
29
12
4
4
16
16
177
178
2970
2800
359486 359164
orange 230 62 1 4 180 2852 — 356985
Cannister
c
Kit
1-6
500 — — — 183 2880 359481 359232
PN GS6-TB-003MD
Page 17
GH-3.8 Rotor
Description
Table 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the GH-3.8 Rotor (Continued)
BOTTLE ADAPTERS (polypropylene)
r
at
max
Bottle
Bottom
(mm)
RCF at
Maximum
Speed
(x g)
Adapter
Part Number
(qty one)
(use with 349946)
Color
Code
orange
(conical)
Nominal
Bottle
Volume
(mL)
Nominal
Bottle
Diameter
(mm)
Maximum
Number
Bottles
in Rotor
230 62 4 195.1 3070 356983
yellow 250 62 4 195.1 3070 349946
warm red
250
d
62 4 203.2 3200 349849
(conical)
light
purple
e
500 70 4 200.2 3150 349945
blue 750 96 4 195.2 3070 349846
a. Tube retainers and adapter plates are sold individually.
b. Tube racks used with Aerosolve cannisters do not provide full tube support; some manufacturers’ plastic and glass tubes cannot withstand the
maximum forces generated by this rotor when used in these racks. Beckman Coulter highly recommends that you pretest other manufacturers’
tubes (in the appropriate Aerosolve cannister labware) using water samples.
c. Cannisters and lids are made of polyphenylsulphone; O-rings are ethylene-propylene. Cannister kit includes the pad that must be used beneath
the cannister in the GH-3.8 rotor; sold in sets of two or four.
d. Corning polypropylene bottle.
e. Light purple adapter replaces the previous tan adapter. See note under Symmetric and Balanced Loading for weight difference information.
Table 3 Beckman Coulter Tubes and Bottles for the GH-3.8 Rotor
Adapter
Dimensions
Volume
(mL)
Description
Part
Number
Set of Two Set of Four
OPEN-TOP TUBES
16 × 76 mm 10 polypropylene 355640
16 × 76 mm 10 polycarbonate 355630
16 × 76 mm 10
18 × 98 mm 15 polycarbonate 342080 359473
18 × 98 mm 15 polyethylene 342081 359473 359152
18 × 98 mm 15 polypropylene 342082 359473 359152
29 × 104 mm
29 × 103 mm
50
50 polypropylene 357007
29 × 103 mm 50
stainless steel
polycarbonate,
graduated
polycarbonate 363647
301108
363075
(pkg/8)
349471
359484
359471
359484
359471
359484
359474
359486
359474
359486
359474
359486
a
a
a
a
a
a
359150
359162
359150
359162
359150
359162
359152
359153
359164
359153
359164
359153
359164
a
a
a
a
a
a
PN GS6-TB-003MD
1-7
Page 18
GH-3.8 Rotor
Description
Table 3 Beckman Coulter Tubes and Bottles for the GH-3.8 Rotor (Continued)
Dimensions
Volume
(mL)
Description
Part
Number
TUBES WITH SNAP-ON CAPS
11 × 38 mm 1.5 polypropylene 357448
11 × 38 mm 1.5 polypropylene 356090
11 × 38 mm 1.5
11 × 38 mm 1.5
11 × 38 mm 1.5
11 × 38 mm 1.5
blue
polypropylene
green
polypropylene
yellow
polypropylene
orange
polypropylene
356091
356092
356093
356094
Adapter
Set of Two Set of Four
359469
b
354511
354495
a
359148
359469
b
354511
354495
a
359148
359469
b
354511
354495
a
359148
359469
b
354511
354495
a
359148
359469
b
354511
354495
a
359148
359469
b
354511
354495
a
359148
11 × 38 mm 1.5
11 × 39 mm 1.8
polypropylene
(cap separate)
white
polyethylene
343169
340196
29 × 103 mm 50 polypropylene 357005
29 × 103 mm 50 polycarbonate 363664
CONICAL TUBES
17 × 120 mm
15
62 × 141 mm 230
62 × 141 mm 230
polypropylene
(graduated)
polycarbonate
(with cap)
polypropylene
(with cap)
355663
356987
356989
359469
b
354511
354495
a
359148
359469
b
354511
a
354495
359474
a
359486
359474
a
359486
359472
a
359487
c
c
349946
349946
b
b
359148
359153
359164
359153
359164
359151
358991
356983
356985
356983
356985
a
a
a
a
a
1-8
PN GS6-TB-003MD
Page 19
Table 3 Beckman Coulter Tubes and Bottles for the GH-3.8 Rotor (Continued)
GH-3.8 Rotor
Description
Dimensions
Volume
(mL)
Description
Part
Number
BIOVIALS
14 × 55 mm 4 polypropylene 566353
BOTTLES
29 × 104 mm 50
29 × 104 mm 50
29 × 104 mm 50
29 × 104 mm 50
28.5 × 107 mm 50
62 × 141 mm 230
62 × 141 mm 230
polycarbonate
(with cap assembly)
polycarbonate
(with screw cap)
polypropylene
(with cap assembly)
polypropylene
(with screw cap)
Teflon with high-speed
screw cap
conical, wide-mouth
polycarbonate
conical, wide-mouth
polypropylene
361693
357002
357001
361694
357003
363076
356987
356989
polycarbonate
62 × 136 mm 250
(with screw cap,
355673
round bottom)
Adapter
Set of Two Set of Four
359470
344517
a
359149
(each)
359474
359486
a
359474
359486
or
359486
a
359474
a
359474
359486
a
359474
359486
a
359153
359164
359153
359164
359153
359164
359153
359164
359153
359164
a
a
a
a
a
356983
c
(use w/
—
349946)
356983
c
(use w/
—
349946)
349946
b
—
PN GS6-TB-003MD
wide-mouth
62 × 122 mm 250
polycarbonate
(with cap)
wide-mouth
62 × 122 mm 250
polypropylene
(with cap)
62 × 122 mm 250
62 × 120 mm 250
69 × 160 mm 500
69 × 159 mm 500
wide-mouth
polycarbonate
wide-mouth
polypropylene
polypropylene
(with cap assembly)
polypropylene
(with cap)
69 × 159 mm 500 polypropylene
356013
356011
358275
358326
355607
355665
355650
c
c
c
349946
349946
349946
349946
349945
349945
349945
b
b
b
b
b
b
b
—
—
—
—
—
—
—
1-9
Page 20
GH-3.8 Rotor
A
B
C
D
E
F
G
H
Description
Table 3 Beckman Coulter Tubes and Bottles for the GH-3.8 Rotor (Continued)
Adapter
Dimensions
Volume
(mL)
Description
Part
Number
Set of Two Set of Four
BOTTLES (Continued)
96 × 130 mm 750
96 × 130 mm 750
a. Adapter used in Aerosolve cannister (part number 359232).
b. Tube retainers and adapter plates are sold individually.
c. These tubes cannot be run with bucket covers in place.
d. Replacement bottle cap part number is 356263 (set of 6).
e. Do not load the 750-mL bottle directly into the bucket; always use the adapter.
polycarbonate
(with screw cap
polypropylene
(with screw cap
d
)
d
)
358299
356855
e
e
349846
349846
b
b
—
—
CAUTION
Do not run chloroformed samples in Aerosolve cannisters. Chloroform vapors can
damage the cannister material.
Micro Plus Multiwell Plate Carriers
Figure 1 The Micro Plus Multiwell Plate Carrier
Micro Plus multiwell plate carriers (see Figure 1) are used by installing them on the pivot pins in
place of the buckets normally used with the rotor. Micro Plus carriers are made of aluminum, and
are black-anodized for corrosion resistance. The design of this carrier allows higher operating
speeds than were possible in older carrier designs. A blue thermoplastic base is used to house the
multiwell plates. A flexible plastic pad between the base and the plate(s) supports the plates during
a run. Each Micro Plus carrier will accommodate a deep-well multiwell plate, or can run up to three
96-well microplates (when more than one are run, one plate is placed directly on top of the other).
Refer to Table 4 for a list of labware that can be used with the carrier. Multiwell plates will break if
g-forces are too high. Rotor speed must not exceed 3250 rpm when Micro Plus carriers are run, and
additional speed reduction is required if maximum load limits (500 grams per carrier) are exceeded.
1-10
PN GS6-TB-003MD
Page 21
Installation
The Rotor Yoke
GH-3.8 Rotor
Installation
NOTE
1
2
Before the first use of a new rotor, lubricate the rotor pins and bucket pin sockets as described under
Maintenance. Also, remove the lubrication decal over the rotor drive hole (in the yoke) and lubricate the
inside of the drive hole as described under Maintenance.
Note the orientation of the pin on the drive shaft and the groove in the bottom of the rotor yoke
(see Figure 2).
Holding the rotor yoke with both hands, carefully lower it onto the drive shaft. Make sure the
pin on the drive shaft fits into the groove in the bottom of the rotor yoke.
CAUTION
Never drop the yoke onto the shaft. The shaft can be bent or broken if the yoke is
dropped onto it.
PN GS6-TB-003MD
1-11
Page 22
GH-3.8 Rotor
Installation
Table 4 Labware for Use with Micro Plus Carriers
Description Volume
Multiwell plate,
96-well, nonsterile
Deep-well polystyrene
plate, 96-well, nonsterile
Deep-well polystyrene
plate, 96-well, sterile
Deep-well
polypropylene plate,
96-well, nonsterile
Deep-well
polypropylene plate,
96-well, sterile
Square-well
polypropylene plate
Microfuge tube,
polypropylene, clear
300 μL/well
1 mL/well
1 mL/well
1 mL/well
1 mL/well
2 mL/well
1.5 mL
Part
Numbers
609844
(pkg/100)
267001
(pkg/24)
267004
(pkg/24)
267006
(pkg/24)
267007
(pkg/24)
140504
(pkg/24)
357448
(pkg/500)
a
Cap strip, nonsterile
Cap strip, sterile
Aluminum foil lid
Cap strip, nonsterile
Cap strip,
Aluminum foil lid
Cap strip, nonsterile
Cap strip,
Aluminum foil lid
Cap strip,
Cap strip,
Aluminum foil lid
Aluminum foil lid
Rack insert, white
Required Accessory
RackDescription Part Number
———
a
267002 (pkg/10)
sterile
sterile
nonsterile
sterile
a
b
a
b
a
b
a
b
b
267005 (pkg/10)
538619 (pkg/100)
a
267002 (pkg/10)
267005 (pkg/10)
538619 (pkg/100)
a
267002 (pkg/10)
267005 (pkg/10)
538619 (pkg/100)
a
267002 (pkg/10)
267005 (pkg/10)
538619 (pkg/100)
538619 (pkg/100) —
373696
(pkg/25)
—
—
—
—
373661
(holds
24 tubes)
Microfuge tube,
polypropylene, clear
Microfuge tube,
polypropylene, blue
Microfuge tube,
polypropylene, green
Microfuge tube,
polypropylene, yellow
Microfuge tube,
polypropylene, orange
a. Caps are optional.
b. Requires soft rubber roller (4-in.), part number 538618, for installation.
1.5 mL
1.5 mL
1.5 mL
1.5 mL
1.5 mL
356090
(pkg/500)
356091
(pkg/500)
356092
(pkg/500)
356093
(pkg/500)
356094
(pkg/500)
1-12
Rack insert, white
Rack insert, white
Rack insert, white
Rack insert, white
Rack insert, white
373696
(pkg/25)
373696
(pkg/25)
373696
(pkg/25)
373696
(pkg/25)
373696
(pkg/25)
373661
(holds
24 tubes)
373661
(holds
24 tubes)
373661
(holds
24 tubes)
373661
(holds
24 tubes)
373661
(holds
24 tubes)
PN GS6-TB-003MD
Page 23
GH-3.8 Rotor
Drive Shaft
Pin
Groove
Installation
Figure 2 Rotor Yoke and Centrifuge Drive Shaft. Be sure the pin in the
drive shaft is in the groove in the rotor yoke.
3
Before using the tie-down nut, check it for damage such as distortion, splitting, or stripped
threads; replace it if it is damaged. If no damage is apparent, lightly coat the O-ring (927571) in
the base of the nut with silicone vacuum grease (335148). Do not use any type of lubricant or
grease on the threads of the tie-down nut.
4
Screw the tie-down nut (366642) onto the centrifuge drive shaft.
WARNING
Do not substitute a metal fastener for the plastic tie-down nut (366642) supplied
with this rotor. If a tie-down nut comes off the shaft during centrifugation, the
plastic nut furnished will break apart in the chamber, causing minimal damage to
the instrument. A loose metal object, however, could substantially damage the
rotor, chamber, and lid, and could potentially escape the chamber into the
laboratory, causing personal injury or property damage.
PN GS6-TB-003MD
1-13
Page 24
GH-3.8 Rotor
O-Ring
O-Ring Groove
Torquing Bar
Installation
5
Insert the torquing bar (356036) through the cross-drilled hole in the tie-down nut and turn the
nut to the right (clockwise) until it is tight. Do not overtighten the tie-down nut.
NOTE
and the tie-down nut is tight before each run.
Figure 3 Rotor Tie-Down Nut and O-Ring
Buckets or Carriers
1
Place buckets or carriers over the pivot pins on the rotor yoke. Fill all four positions on the yoke
with buckets and/or carriers.
If the rotor is left in the centrifuge between runs, make sure the rotor is seated on the drive shaft
CAUTION
All four positions on the rotor yoke must contain either a bucket or a carrier during
a run. Never run the rotor with only two positions filled.
2
Make sure buckets or carriers are properly seated by gently swinging them on the pivot pins.
You can use four buckets, four carriers, or two buckets and two carriers (with like components
positioned opposite each other). If two buckets and two carriers are used, reduce run speed to the
speed allowed for multiwell plate carriers (refer to Speed Derating, below).
1-14
PN GS6-TB-003MD
Page 25
Loading the Rotor
Working with Physiological Fluids
WARNING
Handle body fluids with care because they can transmit disease. No known test
offers complete assurance that they are free of micro-organisms. Some of the
most virulent — Hepatitis (B and C) viruses, HIV (I–V), atypical mycobacteria, and
certain systemic fungi — further emphasize the need for aerosol protection.
Take appropriate safety precautions when handling toxic, pathogenic, or other
hazardous materials.
When working with potentially hazardous materials, always fill or open containers in an
appropriate hood or biological safety cabinet. Three levels of containment are offered by
Beckman Coulter, and can be used singly or combined, depending upon your application.
GH-3.8 Rotor
Loading the Rotor
1. Capped tubes or bottles are designed to provide fluid containment. We strongly recommend
that all containers carrying physiological fluids be capped to prevent leakage.
2. Bucket covers for the GH-3.8 rotor help to contain fluids within the bucket in the event of tube
breakage or blood-bag failure.
3. Aerosolve cannisters are designed to minimize the possibility of aerosol (and fluid) leakage
during centrifugation.
Symmetric and Balanced Loading
To ensure optimal performance and stability, the GH-3.8 rotor must be loaded symmetrically (see
Figure 4). Two factors affect symmetric loading:
• The buckets must be loaded symmetrically with respect to their pivotal axes.
• The rotor should be loaded symmetrically with respect to its center of rotation.
This means that for best results you should load opposing buckets or carriers with the same type of
labware containing the same amounts of fluid of equal density. Additionally, opposing buckets and
their contents should weigh approximately the same (within 6 grams). Do not exceed the rated
maximum load for either buckets (1000 grams each, including labware and sample) or Micro Plus
carriers (500 grams each, including base, pad, plate(s), and sample).
PN GS6-TB-003MD
1-15
Page 26
GH-3.8 Rotor
Pivotal Axis
Pivotal Axis
of Bucket
Center of
Rotation
BALANCED LOAD
CORRECT
Examples of Symmetrically Loaded Trays
(load opposite trays the same way)
Examples of Nonsymmetrically
Loaded Trays
INCORRECT
UNBALANCED LOAD
Carrier
Plate
A
B
C
D
E
F
G
H
Loading the Rotor
Figure 4 Examples of Correctly and Incorrectly Loaded Buckets and Carriers. Contents of opposing buckets
must be the same and each bucket must be balanced on its pivotal axis.
1-16
PN GS6-TB-003MD
Page 27
GH-3.8 Rotor
Loading the Rotor
CAUTION
If only two buckets are loaded with blood bags, bottles, or modular disk adapters,
the other two buckets should contain at least a minimal “blank” load (such as
empty modular disk adapters) to achieve optimal results and to avoid rotor
imbalance. If only two blood bags are being run and modular disk adapters are not
available, we recommend using two water-filled blood bags in cups in the empty
buckets. Placing empty blood bag cups or bottle adapters in the open buckets
does not provide enough weight to prevent possible imbalance.
Beckman Coulter supplies buckets in weight-matched sets to make balancing easier (the weight and
warranty expiration date are marked on the side of each bucket). Modular disk adapters are also
sold in weight-matched sets. However, there are variances in weight between sets, as well as
variance in weight between previously purchased adapters. To prevent accidental imbalance it is
important to keep matched sets of adapters together and to weigh other adapters to be sure they
are approximately the same. Marking matched sets will help you keep them together.
NOTE
The light purple 500-mL adapters (349945) replace the previous tan adapters. There is a weight
difference between the old tan adapters and the current purple adapters. If you run a mix of the purple
and tan adapters, be sure to run matching colors in opposing positions or rotor imbalance could occur.
It is not necessary to completely fill all tubes, positions in adapters, or wells in multiwell plates;
however, partially filled adapters or multiwell plates must be balanced with respect to the pivotal
axis of the bucket or carrier as discussed below.
In adapters, each tube should be placed so that its weight is balanced by a tube in a diametrically
opposite position across the pivotal axis in the same adapter (see Figure 4). Adapters placed in
opposing buckets should also be filled the same way.
If you must run only one tube in an adapter, be sure this tube rests over the bucket's pivotal axis.
• Use the center hole of the adapter, if one is available.
• Use a side hole in line with the bails (vertical supports).
• Turn the adapter so that the tube rests across the pivotal axis of the bucket. The bails will no
longer be in line with the pivotal axis; check that they do not interfere with the proper swing
of the bucket.
NOTE
Be sure to run a tube of the same approximate weight in the same configuration in the opposite
bucket.
In multiwell plate carriers, samples should be loaded into the wells symmetrically with respect to
the pivotal axis of the carrier (the pivotal axis runs parallel to the crossbar), and opposing carriers
should contain the same loads (see Figure 4).
PN GS6-TB-003MD
1-17
Page 28
GH-3.8 Rotor
Bails
Bails
J6
GH-3.8
Loading the Rotor
NOTE
Figure 5 The Bails (Vertical Supports) of the GH-3.8 (and GH-3.7) Modular Disk Adapters and the J6 Series
Adapters are Not the Same. Bails for the GH-3.8 are straight and end in a disk; bails for the J6 have curved
ends. Do not intermix adapters (or disks from adapters) between sets.
CAUTION
If multiwell plate carriers are not loaded symmetrically with respect to each
carrier’s pivotal axis, the carriers could come off the rotor pivot pins and could
damage the rotor and the centrifuge.
The same adapters can be used in the GH-3.8 and GH-3.7 rotors (see Table 2). However, do not
intermix adapters (or their individual parts) from Beckman Coulter J6 series rotors with those for the
GH-3.8 rotor. While the adapters are very similar in appearance (see Figure 5), they have very different
weights. J6 adapters have bails that are curved at the top; bails for the GH-3.8 adapters are straight. Keep
J6 and GH-3.8 disks and bases separate — mixing them can cause imbalance.
1-18
PN GS6-TB-003MD
Page 29
GH-3.8 Rotor
AT REST
Proper Weight Distribution
Improper Weight Distribution
AT SPEED
Loading the Rotor
During a run, buckets and carriers swing 90 degrees from their at-rest position. The pivotal axis of
a bucket or carrier can be imagined as a line extending across the bucket or carrier from one pivot
pin to the other. If a bucket or carrier is loaded so that its weight is unequally distributed on either
side of its pivotal axis, it will not hang vertically at rest and, more importantly, may not swing to a
horizontal position during a run (see Figure 6). As a result, extra stress will be placed on the bucket,
carrier, tubes, and/or multiwell plates during the run, increasing the possibility of breakage or
rotor imbalance.
Figure 6 Examples of the Effects on the Horizontal
Swing of Buckets Caused by Proper and Improper
Weight Distribution
Using Buckets
PN GS6-TB-003MD
You can load buckets before or after they are installed on the rotor yoke. In either case, we
recommend filling the appropriate labware first and then loading the labware into the buckets. This
is especially important when using blood bags — you can trip the imbalance detector in the
centrifuge by pushing blood bags into cups within buckets installed in the rotor.
Refer to Tables 2 and 3 to determine the appropriate labware required for your application.
Whether you are running tubes, bottles, or blood bags, you must load the buckets symmetrically
around the center of rotation and each bucket must be loaded symmetrically with respect to its
pivotal axis (see Symmetric and Balanced Loading, above).
1-19
Page 30
GH-3.8 Rotor
Base
Disks
Bails
Loading the Rotor
Assembling Modular Disk Adapters
Refer to Table 2 to determine which color adapter is required for the tubes you are using and the
number of disks needed to support the length of the tubes.
Figure 7 Assembling a Modular Disk Adapter
CAUTION
Buckets are designed to be used only with modular disk adapters, bottle adapters,
and blood bag cups. Do not pour samples directly into buckets, bottle sleeves, or
blood bag cups. Do not load bottles or blood bags directly into the buckets. Do not
use labware that is not specified for use in this rotor.
1-20
1
Select the appropriate adapter base (from Table 2) and attach a bail to it (see Figure 7).
2
Place the base and bail in an empty bucket on the lab bench (not in the rotor).
3
Position one of the disks in the bucket so that its grooves are aligned with the bail. Push the disk
down until the bail snaps into the grooves.
4
Add more disks in this way until the height of the assembly is nearly as tall as the tubes you will
be using. (If the height of the disks is very tall, you may have to push the bail into the grooves
of the top disks by hand.) Remove or add disks to the bail to accommodate shorter or longer
tubes. If the tubes fit too snugly in the adapter’s rubber base, apply a light film of powder, such
as talcum powder, to prevent the tubes from sticking.
PN GS6-TB-003MD
Page 31
GH-3.8 Rotor
Pivot Pin
Loading the Rotor
Loading Tubes in Adapters
When placing tubes in modular disk adapters, it is important to make sure that they will not contact
the rotor yoke during a run.
• One way to do this is to place empty tubes in an adapter, place the adapter in a bucket on the
rotor yoke, and manually swing the bucket to the horizontal position, making sure that all
tubes clear the yoke.
• Another method is to place the adapter in a bucket and position a bucket cover over the tubes,
checking that tubes do not touch the cover.
If all positions in an adapter are not filled, the adapter should be loaded symmetrically with respect
,
to its pivotal axis (see Symmetric and Balanced Loading
above).
Loading Adapters in the Buckets
After placing tubes in the disk adapters, lower the adapters into the buckets so that the bails line up
with the rotor pivot pins (see Figure 8). If only two loaded adapters are run, place them in opposing
buckets. The other two buckets should contain empty adapters to prevent imbalance. (See
Symmetric and Balanced Loading, above.)
Figure 8 Installing an Adapter in a Bucket. Be sure the bails (vertical supports)
line up with the bucket pivot pins.
PN GS6-TB-003MD
1-21
Page 32
GH-3.8 Rotor
Loading the Rotor
Using Bottles in Adapters
Load bottles into their appropriate adapters (see Table 2), then place the filled adapters in the rotor
buckets. If only two bottles are run, place them in opposite buckets. Make sure the other two
buckets contain at least a minimal “blank” load (such as empty modular disk adapters) to prevent
rotor imbalance. If modular disk adapters are not available, use two water-filled bottles in adapters
to balance the load. (See Symmetric and Balanced Loading, above.)
Using Blood Bag Cups
NOTE
The yellow 90-mm cup (356856) accommodates up to two single bags or one double pack; the orange
97-mm cup (356857) supports either one triple or one quad pack. Do not pour liquid directly into blood
bag cups. Fit blood bags into cups before loading the cups into the rotor buckets — you can trip the
im balance d etector i n the cen trifuge b y pushing blood bag s into cu ps within b uckets in stalled in the
rotor. Load the blood bag cups as follows:
This rotor is suitable for obtaining cell-free plasma or for cell packing, not for separating blood
components.
1
Load the bags as far down into the cups as possible
NOTE
2
Sandwich the tubing between the blood bag and any transfer packs (see Figure 9).
3
Make sure the loaded blood bag cups opposite each other on the rotor yoke are approximately
the same weight (within 1 gram). Soft pliable balancing pads that will not puncture blood bags
(see the Supply List) can be used if necessary to maintain weight balance.
4
Place loaded cups into rotor buckets. To reduce the possibility of bag breakage, align the blood
bag seam with the rotor pivot pins (see Figure 9) with the label facing out.
Make sure the bags stay as vertical as possible, with no folds at the top or corners. If folds are
present, blood cells could remain in the folds and then mix with the plasma when the bag is removed
1-22
PN GS6-TB-003MD
Page 33
GH-3.8 Rotor
Blood Bag Label
Blood Bag Label
Blood Bag
Label
Blood Bag Label
Blood Bag Seam
Aligned with
Pivotal Axis of
Bucket
Sandwich Coiled
Tubing between
Transfer Bags
and Filled
Blood Bag
Ports
Blood Bag
Seam
Blood Bag
Label
Blood Bag Cup
Loading the Rotor
5
Place bucket covers on rotor buckets to prevent blood bags or tubing from contacting the rotor
yoke during centrifugation.
Figure 9 Blood Bag Loading Procedures
CAUTION
If bucket covers are not used, make sure the superstructure of the blood bag
protruding from the cup does not inhibit the bucket from reaching its horizontal
position. If it does, remove the cup from the rotor and reposition the blood bag so
that it seats further into the cup. Allowing the blood bags to contact the rotor yoke
during centrifugation can cause the bucket to come off the pivot pins and can
seriously damage both the rotor and the centrifuge.
If only two filled cups are run, place them in opposing buckets. The other two buckets should
contain similar “blank” loads to prevent imbalance (either empty modular disk adapters or waterfilled blood bags in cups).
Bucket Covers
Beckman Coulter recommends that you use bucket covers (360586) to help contain spills and glass
particles in the event of tube breakage. Bucket covers can be used with all blood bags, bottles
(except 230-mL conical), and tubes up to 130 mm long. They are not designed to contain aerosols.
NOTE
Because of the shape of the covers, 130-mm tubes can be used only in the center cavities of the
adapters when covers are used.
PN GS6-TB-003MD
1-23
Page 34
GH-3.8 Rotor
Insert
O-ring here
Cover
Spring
Latch
Bucket
Snap Down
Pin Socket
Loading the Rotor
Install the covers as follows:
1
2
3
Figure 10 Proper Placement of O-Ring
Make sure that the bucket and the bucket cover surface are clean, dry, and undamaged.
Make sure that the O-ring (961648) is in good condition and lightly coated with silicone vacuum
grease (335148)
Place the O-ring on the ledge inside the bucket (see Figure 10).
1-24
4
Place the cover on the bucket with the latches perpendicular to the pin sockets (see Figure 11).
5
Snap the latches down and secure them under the bucket rim.
Figure 11 Securing Bucket Cover Latches
PN GS6-TB-003MD
Page 35
GH-3.8 Rotor
Loading the Rotor
Using Aerosolve Cannisters
CAUTION
Do not run chloroformed samples in Aerosolve cannisters. Chloroform vapors can
damage the cannister material.
Aerosolve cannisters can be used as wide-mouth bottles or with tubes in racks that are specially
designed to fit in the cannisters. Table 2 lists the Aerosolve tube racks and the number and sizes of
tubes they accommodate. When Aerosolve cannisters are used in the GH-3.8 rotor buckets, pads
(361269) must be placed beneath the cannisters in the buckets to provide support and prevent the
cannisters from being damaged during centrifugation. These pads are included in the GH-3.8
Aerosolve cannister kits (359232).
CAUTION
Be sure to remove the pad when you remove the cannister; placing other
accessories on top of the pad could cause tube breakage, rotor imbalance,
improper run results, or rotor mishap. Tubes, bottles, or blood bags placed in
labware that rests on Aerosolve cannister pads will be closer to the rotor yoke and
can interfere with the proper swing of the bucket.
If using tube racks, load them symmetrically (refer toFigure 4). If only two cannisters are being run,
place the cannisters in opposite buckets; place empty adapters (or “blank” loads) in the two empty
buckets for balance.
Read publication TJ6-TB-011, supplied with the cannister, for complete information regarding its
use and maintenance.
PN GS6-TB-003MD
1-25
Page 36
GH-3.8 Rotor
A
B
C
D
E
F
G
H
Deep-well Plate
Pad
Base
Carrier
Loading the Rotor
Using Micro Plus Multiwell Plate Carriers
Micro Plus plate carriers (see Figure 12) can be used in place of the buckets normally used with the
rotor. (For complete information about the carriers, see publication GS6-TB-011, which
accompanies the carriers.) If only two carriers are run, they must be installed opposite each other
in the rotor, and either two buckets or two other carriers must also be run (they need not be loaded)
to prevent rotor imbalance. The rotor should never be run with only two buckets or two carriers
installed. Opposing Micro Plus carriers must balance to within 10 grams. (See Symmetric and
Balanced Loading, above.)
Figure 12 The Micro Plus Multiwell Plate Carrier, Base, Pad, and Deep-well Plate
1-26
NOTE
If symmetrical pelleting is required, use round (“U-bottom”) or “V-bottom” multiwell plates
(commercially available from many suppliers) for best results.
Load a multiwell plate into a carrier as follows.
1
To prevent plate breakage during centrifugation, place the flexible plastic pad (362390), ridged
side up, into the flat, indented area of the blue base (361302).
2
Place the filled labware on top of the pad, being careful not to spill the contents. If you are using
two or three microplates, place a 96-well cap strip (267002) between the plates to prevent plate
breakage during centrifugation.
NOTE
3
Slide the base, pad, and plate assembly into the carrier until the base locating feet click into
If running stacked polystyrene plates, place a 96-well cap strip (267002) beneath the bottom
plate, as well as between each set of plates.
place.
PN GS6-TB-003MD
Page 37
Operation
speed (3750 rpm)
1000 grams
heaviest load in grams
------------------------------------------- ------------=
speed (3250 rpm)
500 grams
heaviest load in grams
------------------------------------------- ------------=
GH-3.8 Rotor
Operation
IF YOU USE MORE THAN ONE GH-3.8 ROTOR
Complete sets of buckets, Micro Plus plate carriers, and labware may be used in any GH-3.8 rotor.
Remember the following points:
• Buckets are sold in weight-matched sets. Keep matched sets together and check
their weights prior to use to avoid centrifuge imbalance.
• Modular disk adapters and tube racks for Aerosolve cannisters are sold in weight-matched sets. Mark
sets of adapters so that they can be easily identified. When cleaning adapters, do not mix sets. Check
the weight of adapters prior to use.
• Do NOT intermix adapters (or their components) from Beckman Coulter’s J6 series rotors with those
used in the GH-3.8 rotor. These adapters are similar in appearance (see Figure 5) but have very
different weights and functional characteristics. Mixing them will cause centrifuge imbalance and
potential instrument damage.
Speed Derating
The maximum allowable run speed (3750 rpm) listed in the rotor specifications is for operation
when all conditions are within the standard specifications (using four buckets). Maximum
allowable run speed for the GH-3.8 rotor must be reduced in any of the following circumstances.
• Maximum allowable run speed when Micro Plus plate carriers are used is 3250 rpm. If two
buckets and two carriers are used, reduce run speed to the speed allowed for carriers.
• If the weight of the load in a bucket exceeds 1000 grams, or if the solution density is greater than
1.2 g/mL, use the following square-root reduction formula to determine the allowable rotor
speed:
• If the weight of the load in a Micro Plus multiwell plate carrier exceeds 500 grams, including base,
pad, labware, and sample, use the following square-root reduction formula to determine the
allowable rotor speed:
(1)
(2)
PN GS6-TB-003MD
• Reduce rotor speed if the desired rotor temperature (when run in a refrigerated centrifuge) is
lower than -3°C and ambient temperature is higher than 25°C.
1-27
Page 38
GH-3.8 Rotor
Operation
• When certain plastic tubes made by other manufacturers are used in Aerosolve cannister tube
racks, you must reduce speed to prevent tube deformation or breakage, as these racks do not
provide full tube support. Beckman Coulter recommends that you pretest other manufacturers’
tubes in these racks using water samples to determine run speed and duration reductions to
avoid tube deformation or breakage.
• When using certain glass tubes, you must reduce rotor speed to prevent tube breakage. Table 5
indicates recommended g-force limits to minimize breakage and is intended as a guide only.
Because the strength of glass and plastic tubes can vary between lots, and will depend on
handling and usage, Beckman Coulter highly recommends that you pretest these tubes in the
appropriate labware in the GH-3.8 rotor using water samples to determine optimal operating
conditions.
NOTE
Temperature Limits
• Beckman Coulter plastic containers have been centrifuge tested for use at temperatures
between 2° and 25° C. For centrifugation at other temperatures, pretest containers under
anticipated run conditions.
• If plastic containers are frozen before use, make sure that they are thawed to at least 2° C before
centrifugation.
Run Procedure
Scratches (even microscopic ones) significantly weaken glass tubes.
WARNING
Operator error or tube failure may generate aerosols. Toxic, pathogenic, or other
hazardous materials must not be run in this rotor unless all appropriate safety
precautions are taken. Also see Working with Physiological Fluids, above.
CAUTION
1-28
Make sure the rotor is seated on the drive shaft and the tie-down nut is tight
before each run.
Refer to the appropriate instrument instruction manual for centrifuge operation. It is best to
precool or warm the rotor to the desired temperature before installing it in the centrifuge. Table 6
and Table 7 indicate approximate acceleration and deceleration times for this rotor in both 60-Hz
and 50-Hz instruments.
PN GS6-TB-003MD
Page 39
GH-3.8 Rotor
Operation
Table 5 Recommended g-Force Limits to Minimize Breakage of Certain Glass Tubes and Bottles Made by
Other Manufacturers in the GH-3.8 Rotor
Tube Description
Pyrex, Kimax, B-D, or Dispo
(RB or FB)
Pyrex (LT)
B-D Vac (RB, plain)
Kimax (RB)
Pyrex 9820 (RB)
B-D SST (RB, SB)
B-D Vac (RB, plain)
B-D SST (RB, SB)
B-D SST (RB, plain)
Kimax 45049 (RB)
Kimax 45200 (LT, G, SC)
Pyrex 9820 (RB)
Volume
(mL)
3
or less
3
a
5
5
a
5
a
7
a
7
a
10
a
10
10
10
10
Dimensions
(mm)
10 × 75
11 × 75
12 × 75
12 × 75
12 × 75
13 × 100
13 × 100
16 × 100
16 × 100
16 × 100
17 × 116
16 × 100
Safe g
Force
(RCF)
max
max
max
max
max
max
max
3000
max
max
max
max
Safe
Speed
(RPM)
3750
3750
3750
3750
3750
3750
3750
3600
3750
3750
3750
3750
Modular
Disk Adapter
Color
blue
blue
blue
blue
blue
orange
orange
purple
purple
purple
purple
purple
Pyrex 8140 (LT, G) 12 17 × 117 max 3750 purple
B-D SST (RB, SB)
B-D Vac (RB, plain)
Corex 8060A (LT)
Corex 8080A (LT, G)
Corex 8442 (RB)
Corvac (RB, SB)
Kimax (LT, G)
Pyrex 8500 (ST)
Pyrex 8080 (LT, G)
15
15
15
15
15
15
15
15
15
a
a
16 × 130
16 × 130
17 × 120
a
17 × 120
16 × 115
a
16 × 131
17 × 118
17 × 110
a
17 × 120
2700
2900
max
max
max
max
3000
2700
max
3500
3550
3750
3750
3750
3750
3600
3500
3750
green
green
green
green
green
green
green
green
green
PN GS6-TB-003MD
Pyrex 8320 (ST) 40 29 × 116 max 3750 yellow
Kimax 45212 (ST)
Pyrex 8280 (ST)
a. Most common usage.
Code:
B-D = Becton Dickinson LT = long taper ST = short taper
Dispo = disposable RB = round bottom Vac = Vacutainer (B-D)
FB = flat bottom SB = silicone gel barrier
G = graduated SC = screw capped
50
50
a
a
29 × 118
29 × 115
1000
2500
2100
3300
yellow
yellow
1-29
Page 40
GH-3.8 Rotor
Operation
Table 6 Approximate Acceleration and Deceleration Times for the GH-3.8 Rotor in Allegra 6 Series,
GS-6 Series, and Spinchron Series Centrifuges
a
Deceleration (L)
(seconds)
Instrument
Acceleration
(seconds)
Deceleration (H)
(seconds)
60-Hz Nonrefrigerated Centrifuge 160 80 110
60-Hz Refrigerated Centrifuge 140 80 110
50-Hz Nonrefrigerated Centrifuge 160 80 110
50-Hz Refrigerated Centrifuge 160 80 110
a. H = HIGH brake setting; L = LOW brake setting
Table 7 Approximate Acceleration and Deceleration Times for the GH-3.8 Rotor in
GP Series Centrifuges
a
Deceleration (L)
(seconds)
Instrument
Acceleration
(seconds)
Deceleration (H)
(seconds)
60-Hz GP or GPK 165 85 120
60-Hz GPR or GPKR 140 80 125
50-Hz GP or GPK 180 105 120
50-Hz GPR or GPKR 150 85 130
a. H = HIGH brake setting; L = LOW brake setting
a
a
NOTE
Line voltage fluctuations can cause variations in acceleration and deceleration times. The times listed
in Table 6 and Table 7 were achieved with line voltage ratings at 120 volts for 60-Hz instruments and
230 volts for 50-Hz instruments.
NOTE
Do not use high brake when Micro Plus plate carriers are run, as the force of the deceleration will
disturb the contents of the plate. Set the centrifuge brake switch to either LOW or OFF to coast to a stop.
1-30
PN GS6-TB-003MD
Page 41
Care and Maintenance
GH-3.8 Rotor
Care and Maintenance
NOTE
Inspection
• Periodically (at least monthly) inspect the rotor yoke, buckets, and/or multiwell plate carriers,
• Regularly check the condition of the O-ring (927571) in the tie-down nut; if it is worn or
• Regularly check the condition of the Micro Plus plate carrier base (361302) and pad (362390) and
Maintenance
Buckets and Micro Plus carriers are made of anodized aluminum. Do not use sharp tools on them, as
scratches in the anodized surface may lead to corrosion.
Accumulations of dust or other foreign matter can damage rotor pins (resulting in rotor vibrations). To
prevent this, clean the centrifuge bowl daily. Spray the centrifuge bowl with an antistatic solution and
wipe it clean.
especially inside cavities, for rough spots or pitting, white powder deposits — frequently
aluminum oxide—or heavy discoloration. If any of these signs are evident, do not run the rotor.
Contact your Beckman Coulter representative for information about the Field Rotor Inspection
Program and the rotor repair center.
damaged, replace it.
do not use them if there are visible signs of damage.
• Approximately once a week, and after cleaning and/or autoclaving, wipe the rotor pins and pin
sockets (see Figure 13) with a paper towel, then coat with Paint On Graphite Lubricant (977212).
Allow the lubricant to dry for at least 5 minutes before installing the rotor in a centrifuge.
• Before using the tie-down nut (366642), check it for damage such as distortion, splitting, or
stripped threads; replace it if it is damaged. Inspect the O-ring (927571) in the base of the nut
for cracks, replace if damaged. Do not use any type of lubricant or grease on the threads of the
tie-down nut.
• Before first use of the rotor, and after washing, apply Anti-Seize (961660) to the rotor yoke drive
hole as described under Cleaning, below.
PN GS6-TB-003MD
1-31
Page 42
GH-3.8 Rotor
Lubrication Area
(both pin sockets)
Lubrication Points
(all pins)
Care and Maintenance
Figure 13 Lubricating Pivot Pin/Bucket Contact Areas
Cleaning
• Do not use any type of lubricant or grease on the threads of the drive shaft.
• Refer to publication TJ6-TB-011 for information on Aerosolve cannister maintenance.
Refer to Chemical Resistances (publication IN-175) for the chemical compatibilities of rotor and
tube materials. Your Beckman Coulter representative provides contact with the Field Rotor
Inspection Program and the rotor repair center.
NOTE
Do not wash the rotor components or accessories in a dishwasher. Do not soak components in
detergent solution for long periods such as overnight. Do not autoclave the tie-down nut.
The Rotor Components
• If spillage has occurred, or if salt solutions or other corrosive materials are used, clean the rotor
and components immediately. Do not allow corrosive materials to dry on rotor components.
• Approximately once a week (or every 1000 runs) clean the pins and bucket pin sockets, then
lubricate as described under Maintenance.
• Under normal conditions, remove the rotor from the centrifuge at least once a month for
cleaning and lubrication to prevent buildup of residues.
Use a mild detergent such as Beckman Solution 555 (339555), diluted 10 to 1 with water, and a soft
brush to clean the rotor yoke and tie-down nut. The Rotor Cleaning Kit (339558) contains two quarts
of Solution 555 and brushes that will not scratch the rotor.
1-32
PN GS6-TB-003MD
Page 43
GH-3.8 Rotor
Care and Maintenance
Thoroughly rinse the cleaned rotor and components with water and air-dry upside down. Do not use
acetone to dry the rotor. When the rotor is dry, lubricate the bucket sockets and pins as described
under Maintenance. Before reinstalling the rotor, lightly lubricate the drive hole of the rotor with
Anti-Seize (961660) to prevent the rotor from sticking, as follows:
1
Apply the lubricant onto a swab.
2
Draw the coated swab through a paper towel to remove excess lubricant.
3
Lightly coat the inside of the drive hole with the lubricant remaining on the swab.
Buckets
1
Remove the O-ring from the bucket before cleaning.
2
Clean the bucket and O-ring with a mild detergent such as Solution 555 (339555), diluted 10 to
1 with water, then rinse thoroughly and dry completely.
3
Coat the O-ring with a light film of silicone vacuum grease (335148). Place the O-ring on the
ledge inside the bucket.
After cleaning, lubricate bucket pin sockets with Paint On Graphite Lubricant (977212) as described
under Maintenance.
Micro Plus Plate Carriers (Including Base and Pad), Blood Bag Cups, and Bucket Covers
Wash with a mild detergent such as Solution 555 (339555), diluted 10 to 1 with water, and a soft
brush. Thoroughly rinse them and air-dry upside down.
Modular Disk Adapters
1
2
PN GS6-TB-003MD
To disassemble adapters for washing, first pull the bail out of the groove in the disks, then
remove the disks and unsnap the bail from the rubber bottom.
Use a mild detergent such as Solution 555 (339555), diluted 10 to 1 with water, and a soft brush
to scrub the adapters.
1-33
Page 44
GH-3.8 Rotor
Decontamination
3
Rinse and dry, then reassemble.
4
If necessary, apply a light film of silicone vacuum grease (335148) to the grooves on the rubber
bottom to make reassembling easier.
Keep matched sets of adapters together; do not intermix sets.
Aerosolve Cannisters
Refer to publication TJ6-TB-011.
Decontamination
If the anodized aluminum buckets or carriers become contaminated with radioactive material,
decontaminate them using a solution that will not damage their anodized surfaces. Beckman
Coulter has tested a number of solutions and found two that do not harm anodized aluminum:
RadCon Surface Spray or IsoClean Solution
Beckman Coulter does not however, warrant the performance of these products with respect to
their effect on the carriers or their ability to decontaminate these parts.
If the rotor or other components are contaminated with toxic or pathogenic materials, follow
appropriate decontamination procedures as outlined by your laboratory safety officer.
Sterilization and Disinfection
The rotor yoke, buckets, tube adapters, and aluminum parts of multiwell plate carriers can be
autoclaved at 121°C for up to an hour. Blood bag cups, bucket covers, and Aerosolve cannisters
purchased after April 1993 (see the date code on the cannister bottom) can be autoclaved at that
temperature for about 15 minutes. Do not autoclave the plastic tie-down nut or Aerosolve
cannisters purchased prior to April 1993. Ethanol
including those made of plastic. Refer to publication IN-175 for other chemical resistances of tubes,
bottles, and accessories.
IsoClean can cause fading of colored anodized surfaces. Use it only when necessary and remove it
NOTE
promptly from rotor surfaces.
*
(for soaking), and Radiacwash†.
‡
(70%) may be used on all rotor components,
1-34
* In U.S.A., contact Nuclear Associates (New York); in Eastern Europe and Commonwealth States, contact Victoreen
GmbH (Munich); in South Pacific, contact Gammasonics Pty. Ltd. (Australia); in Japan, contact Toyo Medic Co.
Ltd. (Tokyo).
† In U.S.A., contact Biodex Medical Systems (Shirley, NY); internationally, contact the U.S. office to find the dealer
nearest you.
‡ Flammability hazard. Do not use in or near operating centrifuges.
PN GS6-TB-003MD
Page 45
While Beckman Coulter has tested these methods and found that they do not damage components,
no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection
is a concern, consult your laboratory safety officer.
Tube Breakage
If a glass tube breaks, remove the glass very carefully from the adapter and bucket. Imbedded glass
particles that remain in the bucket or adapters can cause tube failure during subsequent runs.
Aerosolve Cannisters
GH-3.8 Rotor
Decontamination
CAUTION
To reduce the potential for corrosion, clean buckets or carriers thoroughly
immediately following a tube or well plate breakage.
WARNING
When working with potentially hazardous materials, open cannisters in an
appropriate hood or biological safety cabinet in case of tube breakage.
If a glass tube breaks in an Aerosolve cannister tube rack, discard and replace the O-ring and rubber
tube cushion in the base of the rack. Imbedded glass particles that remain in the bucket or adapters
can break tubes during subsequent runs.
SST or Corvac Tubes
If an SST or Corvac tube breaks, the tube’s silicone gel barrier material becomes laced with glass
fragments and the tube contents contaminate the entire tube adapter and bucket (or tube rack and
cannister).
If one of these tubes breaks in an adapter in a bucket, use the following procedure.
1
Fill the bucket with water.
2
Autoclave the entire bucket and contents at 121°C for 20 minutes.
3
4
PN GS6-TB-003MD
Decant any remaining water and dislodge the tube adapter (do not remove it) over a waste
container.
Working under a hood and using plastic gloves, immerse the bucket and tube adapter (intact)
in a 2-liter beaker or similar vessel containing ethyl acetate
* Flammability hazard. Do not use in or near operating centrifuges.
*
.
1-35
Page 46
GH-3.8 Rotor
Returning a Rotor
5
6
If one of these tubes breaks in an Aerosolve cannister, use the following procedure.
1
2
Scrub the adapter with a brush and decant the ethyl acetate mixture into a waste bottle for
proper disposal.
Rinse with water and air-dry the bucket and tube adapter.
Working in a biologic safety cabinet and wearing plastic gloves, open the cannister and
immerse the cannister, lid, and contents (intact) in a 2-liter beaker or similar vessel containing
5% sodium hypochlorite (undiluted bleach).
Leave the cannister to soak overnight.
3
Carefully dislodge the tube rack and scrub it with a brush.
4
Decant the bleach into a waste bottle for proper disposal.
5
Rinse with water and air-dry the cannister and tube rack.
Returning a Rotor
Before returning a rotor or accessory for any reason, prior permission (a Returned Goods
Authorization form) must be obtained from Beckman Coulter, Inc. This RGA form, which may be
obtained from your local Beckman Coulter sales office, should contain the following information:
• serial number,
• history of use (approximate frequency of use),
• reason for the return,
• original purchase order number, billing number, and shipping number, if possible,
1-36
• name and phone number of the person to be notified upon receipt of the rotor or accessory at
the factory, and
• name and phone number of the person to be notified about repair costs, etc.
To protect our personnel, it is the customer’s responsibility to ensure that the parts are free from
pathogens and/or radioactivity. Sterilization and decontamination must be done before returning
the parts. Smaller items (such as tubes, bottles, etc.) should be enclosed in a sealed plastic bag.
PN GS6-TB-003MD
Page 47
All parts must be accompanied by a note, plainly visible on the outside of the box or bag, stating that they
are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach
this notification will result in return or disposal of the items without review of the reported problem.
Use the address label printed on the RGA form when mailing the rotor and/or accessories.
Customers located outside the United States should contact their local Beckman Coulter office.
Supply List
Contact Beckman Coulter Sales (1-800-742-2345 in the United States; worldwide offices are listed on
the back cover of this manual) or see the Beckman Coulter Benchtop Rotors, Tubes & Accessories
catalog (BR-9742, available at www.beckmancoulter.com) for detailed information on ordering
rotors, tubes, and accessories. For your convenience, a partial list is given below.
Replacement Rotor Parts
GH-3.8 Rotor
Supply List
Rotor assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360581
Tie-down nut . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366642
O-ring (Buna N rubber) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 927571
Anodized aluminum buckets (set of 4). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360589
Bucket cover kit (set of 2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360585
each set includes:
cover assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360586
O-ring (ethylene-propylene) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 961648
silicone vacuum grease (2 oz) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335148
Micro Plus multiwell plate carriers, includes bases and pads (set of 2) . . . . . . . . . . . . 362394
Micro Plus replacement carriers (set of 2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361304
Replacement bases for Micro Plus carrier, includes bases and pads (set of 2). . . . . . . 361302
Replacement pads for Micro Plus carrier (set of 4) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 362390
PN GS6-TB-003MD
1-37
Page 48
GH-3.8 Rotor
Supply List
Supplies
Modular disk adapters (polypropylene). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 2
Tubes and bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 3
Micro Plus labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 4
Soft rubber roller (for aluminum foil lids) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 538618
Torquing bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 356036
Tube decanter (for use with blue adapter) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 343108
includes:
gasket for 10-mm dia tubes (silicone) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 343106
gasket for 12-mm dia tubes (silicone) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 343107
Adapter plate,1.5-mL (for use with blue adapter) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 354511
Aerosolve cannister kit (set of 4) (each kit includes 1 cannister, 1 1id,
1 O-ring, silicone vacuum grease, and 1 pad) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 359232
Aerosolve cannister O-ring (ethylene-propylene). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 345366
Aerosolve pad (placed beneath cannister in bucket) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361269
Tube racks for Aerosolve cannisters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 2
Neoprene tube cushions
for blue rack (pkg of 24) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 344117
for orange rack (pkg of 18) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 344118
for purple rack (pkg of 18; 12 large, 6 small) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 344119
for dark green rack (pkg of 18; 12 large, 6 small) . . . . . . . . . . . . . . . . . . . . . . . . . 344120
for yellow rack (pkg of 8; 4 large, 4 small) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 344121
Blood bag cup, 90-mm, yellow (polypropylene). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 356856
Blood bag cup, 97-mm, orange (polypropylene) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 356857
Balancing pads (six pads, 3 grams each, red) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 358365
Balancing pads (six pads, 6 grams each, grey) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 358364
Microfuge tube rack inserts:
Blue insert, 13-mm dia (25/pkg) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 373697
Red insert, 12-mm dia (25/pkg). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 373698
White insert, 11-mm dia. for 1.5-mL Microfuge tubes (25/pkg) . . . . . . . . . . . . . . . 373696
Black insert, 10-mm dia (25/pkg) 373699
Rotor Cleaning Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339558
Solution 555 (1 qt). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339555
Silicone vacuum grease (1 oz) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335148
Anti-Seize (1 1/2 oz). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 961660
Paint On Graphite Lubricant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 977212
1-38
PN GS6-TB-003MD
Page 49
Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and
conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election,
by replacement, any defects of material or workmanship which develop within seven (7) years after delivery
of a benchtop centrifuge rotor to the original buyer by Beckman Coulter or by an authorized representative,
provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed
under normal and proper use. Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor
covered by this warranty, Beckman Coulter will supply free of charge all centrifuge parts required for repair.
Conditions
1. Except as otherwise specifically provided herein, this warranty covers the rotor only and Beckman
2. This warranty is void if the rotor has been subjected to customer misuse such as operation or
3. This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the
4. Each bucket, whether purchased with a rotor assembly or purchased separately, is covered by this
Benchtop Rotor Warranty
Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to (i)
tubes, (ii) tube caps, (iii) tube adapters, or (iv) tube contents.
maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual.
rotor characteristics or operated in a Beckman Coulter centrifuge that has been improperly
disassembled, repaired, or modified.
warranty forseven (7) years from the date of purchase, and will be replaced or repaired during such
period according to the terms and conditions of this warranty. The date of manufacture marked on the
bucket may be earlier than the date of purchase, and the expiration date marked on the bucket, which is
seven (7) years after the date of purchase, may be correspondingly offset.
5. Buckets should not be used after the expiration date marked on the bucket. If at the time of purchase the
6. Thermoplastic rotors or components used in some benchtop centrifuges are warranted for one (1) year
Disclaimer
IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS
AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER, INC. SHALL HAVE NO
LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE
MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.
marked expiration date is less than 7 years from the date of purchase, the expiration date becomes the
date of purchase plus seven (7) years. use of a bucket after such expiration date voids Beckman Coulter’s
warranty obligations with respect to any rotor and/or centrifuge in which such a bucket is used.
from date of purchase.
PN GS6-TB-003MD
Warranty-1
Page 50
Benchtop Rotor Warranty
Disclaimer
Warranty-2
PN GS6-TB-003MD
Page 51
Page 52
www.beckman.com
© 2018 Beckman Coulter, Inc.
All Rights Reserved
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