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Cytomics FC 500
With CXP Software
Getting Started
PN 177652A (May 2003)
Beckman Coulter, Inc.
Fullerton, CA 92835pn
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WARNINGS AND PRECAUTIONS
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING
TO OPERATE INSTRUMENT. DO NOT ATTEMPT TO PERFORM ANY PROCEDURE BEFORE CAREFULLY READING ALL
INSTRUCTIONS. ALWAYS FOLLOW PRODUCT LABELING AND MANUFACTURER’S RECOMMENDATIONS. IF IN DOUBT AS
TO HOW TO PROCEED IN ANY SITUATION, CONTACT YOUR BECKMAN COULTER REPRESENTATIVE.
HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS
WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
WARNING - Can cause injury.
CAUTION - Can cause damage to the instrument.
IMPORTANT - Can cause misleading results.
BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETY
STANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO,
PROTECTIVE EYEWEAR, GLOVES, AND SUITABLE LABORATORY ATTIRE WHEN OPERATING OR MAINTAINING THIS OR
ANY OTHER AUTOMATED LABORATORY ANALYZER.
WARNING Risk of operator injury if:
r All doors, covers and panels are not closed and secured in place prior to and during instrument operation.
r The integrity of safety interlocks and sensors is compromised.
r Instrument alarms and error messages are not acknowledged and acted upon.
r You contact moving parts.
r You mishandle broken parts.
r Doors, covers and panels are not opened, closed, removed and/or replaced with care.
r Improper tools are used for troubleshooting.
To avoid injury:
r Keep doors, covers and panels closed and secured in place while the instrument is in use.
r Take full advantage of the safety features of the instrument. Do not defeat safety interlocks and sensors.
r Acknowledge and act upon instrument alarms and error messages.
r Keep away from moving parts.
r Report any broken parts to your Beckman Coulter Representative.
r Open/remove and close/replace doors, covers and panels with care.
r Use the proper tools when troubleshooting.
CAUTION System integrity might be compromised and operational failures might occur if:
r This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals.
r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s
computer with software authorized by Beckman Coulter.
r You install software that is not an original copyrighted version. Only use software that is an original copyrighted version
to prevent virus contamination.
IMPORTANT If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulter
distributor, and, if it is not presently under a Beckman Coulter service maintenance agreement, Beckman Coulter cannot
guarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the most
current information bulletins concerning the product. If you purchased this product from a third party and would like further
information concerning this topic, call your Beckman Coulter Representative
.
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Contents
Welcome, 1
Cytomics FC 500 with CXP Software, 2
Cytometer, 4
FC 500 Workstation, 6
Online Help System, 8
Documents, 10
Reagent System, 12
Installing CXP Software, 12
Logging On to the FC 500 Workstation, 13
Shutting Down the FC 500 Workstation, 14
Revision Status
Initial Issue 5/03
Software version: 1.0
i
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Welcome
This booklet introduces the
Cytomics FC 500 with CXP
software. The FC 500 provides
automated flow cytometric analysis
including DNA analysis,
immunophenotyping, and cellular
subsetting.
The FC 500 includes a state-of-theart Workstation that improves:
■ Usability
■ Workflow efficiency
■ Results management
■ Data analysis.
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The FC 500 can easily adapt to your
laboratory's work flow. This system
has three acquisition modes:
■ MCL Automatic
■ MCL Manual
■ Single Tube.
and a variety of analysis methods:
■ AutoMATOR Batch Analysis
■ MultiFile Analysis
■ Listmode Compensation.
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Cytomics FC 500 With CXP Software
MAIN COMPONENTS
Cytometer
Sends data to the Workstation. The
Cytometer includes:
■ Analyzer that counts, measures and
computes parameters.
■ Power Supply that provides voltages,
vacuum and pressure.
■ MCL that positions the sample tubes in
the carousel for sampling.
■ Laser(s) for fluorescence excitation.
Workstation
Receives data from the Cytometer. Analyzes
and displays numeric results and dataplots.
The Workstation includes:
■ Monitor that displays the software
screens.
■ Computer that runs the instrument
software.
■ Mouse that allows you to navigate
through the software.
■ Keyboard that allows you to input into
the software.
■ Handheld Scanner that reads bar-code
labels (optional).
Cytometer
SYSTEM UPGRADES
Printer
You can use most printers that are
supported by Windows® software and
Beckman Coulter.
2
Power Supply
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Keyboard
Monitor
NEC
MultiSync LCD
1700
M+
Mouse
Computer
3
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Cytometer
The Cytometer counts, measures and computes parameters. These functions are
performed by the Cytometer:
■
Initialize the system.
■ Run samples.
■ Provide visual display on the
Cytometer Indicator Panel.
■ Transmit data to the Workstation.
■ Shutdown the system.
The Power Supply controls voltages required
by the circuitry. It is also the source of
vacuum and pressure for the FC 500.
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The Cytometer Indicator Panel provides a display of several Cytometer activities:
B Ready icon
C Sample flow in process
D Fluorescent channels 1-5
E Waste full icon
F Sheath fluid low icon
C
B
G Forward scatter
H Side scatter
I Low, medium, high flow rate
j Blue laser ON
1) Red laser shutter open
D
E
F
1)
J
I
G
H
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FC 500 Workstation
The FC 500 Workstation receives information from the Cytometer, sets protocols
and panels, analyzes results, then displays, stores and prints the results. The
CXP software gives you immediate access to these features:
Displays the Cytometer Control dialog box to adjust the
cytometer settings and select parameters.
Starts acquisition or continues an acquisition if previously paused.
Pauses the acquisition of the current sample.
Stops the acquisition of the current sample and outputs results.
Runs a carousel of samples one tube at a time.
Runs a single tube in fixed position #10.
Clears the Acquisition Manager to create a new worklist.
Inserts a panel from file into the current worklist.
Places the Cytometer in the Idle mode in order to perform various
cleaning and replacing procedures.
Flushes the sample line and flow cell with sheath fluid to declog
or remove bubbles.
Flushes the sample line and flow cell with cleaning agent.
Performs the Vacuum Line Cleaning procedure.
Accesses the CXP online Help System.
Note: For a complete list of software icons, see the CXP online Help System.
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Some additional analysis features are:
Creates a Surface Plot and selects the Data Source and Format.
Creates an Overlay Plot to compare single parameter histograms.
Creates a Tomogram Plot and selects the Data Source and Format.
Allows batch analysis of stored data using a single file or multifile protocol.
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Online Help System
The FC 500 Workstation has a comprehensive Help System that
includes all the information you need to operate the system.
INFORMATION INCLUDED
■
Procedures for using the FC 500.
■ Concepts to better understand the FC 500.
■ Reference information, such as FC 500 performance
specifications and characteristics.
■ Videos to help illustrate special procedures.
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SPECIAL FEATURES INCLUDED
■ Contents list to locate information in a book-like fashion.
■ Index list for finding key information quickly.
■ Search utility to locate specific information.
■ Glossary list for definitions of terms and fields.
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Documents
Your system comes with this Getting Started booklet. The other documents are
available in hard copy only by order. To order hard-copy documents, contact your
Beckman Coulter Representative.
SHIPPED WITH SYSTEM
■ Getting Started
PN 177652
Brief overview of the FC 500
hardware and CXP
software. This is the
document you are reading
now.
■ Operator Manuals CD-ROM
PN 177628
Use this CD-ROM at any
compatible PC to access the same
Instructions For Use information
that is accessible through the
CXP software online Help System
on the FC 500 Workstation.
This CD-ROM contains PDF
formatted documents for on-site
printing and viewing.
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Note: All information included in the hard-copy documents is available in the CXP
software online Help system.
AVAILABLE BY ORDER
■ Instructions For Use
PN 175571
Day-to-day operating
procedures:
• Startup
• Performance
characteristics
•QC
• Run samples
• Review data
• Shutdown
• The methods the
instrument uses
• Setup
• Error messages
• Troubleshooting
■ Special Procedures
PN 175572
■ Reference
PN 175570
In-depth information about:
• What the instrument does
• Performance
specifications
• Information on
installation
• Safety information
• Predefined panels and
protocols
• Software description
■ Master Index
PN 175653
• Cleaning procedures
• Replacement and
adjustment procedures
This is a combined index
of the hard-copy
documents.
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Reagent System
These reagents are available for use with the Cytomics FC 500
with CXP Software:
■ IsoFlow™ Sheath Fluid
■ COULTER CLENZ® cleaning agent
Quality control materials are also
available for use with the FC 500.
They include:
■ Flow-Check™ Fluorospheres
■ Flow-Set™ Fluorospheres
■ Flow-Count™ Fluorospheres
■ CYTO-TROL™ Control Cells
■ Immuno-Trol™ Cells
■ Immuno-Trol™ Low Cells
■ CYTO-COMP™ Cell Kit
■ QuickCOMP 2 Kit
■ QuickCOMP 4 Kit
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-A
These quality control materials are
For Research Use Only.
Not for use in diagnostic procedures.
■ PC7 (770/488) Setup Kit
■ APC (675/633) Setup Kit
Installing CXP Software
Installing the CXP Software instructions are in the Installation Chapter of the
Operator Manuals CD-ROM. The Installation Chapter appears in the:
■ System Help
■ Reference manual
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Logging On to the FC 500 Workstation
1. Turn on the FC 500 Workstation power.
2. When the
check that:
• The User name field displays
Administrator or your Windows log-in
user name. If not, type in your user name.
Note: When first installing the software, the
User name
• The check box is unchecked.
Enter a password, if needed for your laboratory
3.
and
4.
start the software and power up the Cytometer.
5. At the
[Page 1 of 2] screen:
a. Highlight your operator ID.
b. Enter your password.
c. .
Log On to Windows screen appears,
field must display
.
(CXP Cytometer) to
CXP Cytometer Startup Wizard
Administrator
.
6. Select a protocol, if needed.
7.
Note: In the event that you cannot access the FC 500 online Help System from your
instrument, contact your Beckman Coulter Representative.
.
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Shutting Down the FC 500 Workstation
After you have performed the Daily Shutdown
procedure:
1.
mode.
2. in the main windows to exit.
3.
4. tt (FC OFF) to turn off
5. tt
6. When the Shut Down Windows screen
to put the Cytometer in the Idle
save the current protocol.
the Cytometer.
to shutdown the Workstation.
appears:
Ensure Shut down appears below
What do you want the computer to do?
or to save or not
7.
8. Turn off the monitor and Printer separately.
14
.
Page 19
The BECKMAN COULTER logo, COULTER CLENZ, CYTO-COMP, CYTO-TROL,
Flow-Check, Flow-Set, Immuno-Trol, and IsoFlow are trademarks of Beckman Coulter, Inc.
All other trademarks, service marks, products, or services are trademarks or registered
trademarks of their respective holders.
Copyright © Beckman Coulter, Inc. 2003
All Rights Reserved.
Page 20
Come visit us at www.beckmancoulter.com
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