Beckman Coulter Allegra X-5 Instructions For Use Manual

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Instructions For Use

Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.

Allegra X-5

Centrifuge

Rx Only in the U.S.A.

PN B29071AE April 2017

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Allegra X-5 Centrifuge

Beckman Coulter Eurocenter S.A. 22, rue Juste-Olivier Case Postale 1044 CH - 1260 Nyon 1, Switzerland Tel: +41 (0) 22 365 36 11

Instructions for Use

PN B29071AE (April 2017)

Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

If you have any questions, contact our Customer Support Center.

• Worldwide, find us via our website at

www.beckmancoulter.com/customersupport/support

• In the USA and Canada, call us at 1-800-369-0333.

• Outside of the USA and Canada, contact your local

Beckman Coulter Representative.

Printed in the U.S.A.

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Revision Status

This document applies to the latest software listed and higher versions. When a subsequent software version affects the information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.

Initial Issue 29071AA, 7/2013

Issue AB, 12/2015

Changes were made to: CE Mark

Issue AC, 03/2016

Changes were made to: Australian EMC Compliance Label, Preparation and Loading.

Issue AD, 04/2016

Changes were made to: Symbols and Labels.

Issue AE, 04/2017

Changes were made to: APPENDIX B, Table of Hazardous Substance’s Name and Concentration.

Note: Changes or additions that are part of the most recent revision are indicated in text by a bar in the margin of the amended page.

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Revision Status

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Safety Notice

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate the instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact us.

Alerts for Danger, Warning, Caution, Important, and Note

DANGER

DANGER indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

IMPORTANT

Following the advice in the Important adds benefit to the performance of a piece of equipment or to a process.

NOTE

or servicing of this equipment.

IMPORTANT is used for comments that add value to the step or procedure being performed.

NOTE is used to call attention to notable information that should be followed during installation, use,

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Safety Notice

Safety During Installation and/or Maintenance

WARNING

Risk of personal injury or equipment damage. The centrifuge weighs 77 kg/169 lb. DO NOT attempt to lift or move it without assistance from a lifting device and/or another person.

WARNING

Risk of personal injury. Any servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury. Make sure that the power switch is turned off and the instrument is disconnected from the main power source by removing the Main (power) plug from the outlet receptacle, and refer such servicing to qualified personnel.

Do not replace any centrifuge components with parts not specified for use on this instrument.

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect this equipment to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the instrument.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet

receptacle strip.

• Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into the instrument and damage electrical or mechanical components.

• Work on the power supply system must be performed by certified electricians.

• Inspect the electrical equipment of the unit regularly. Defects such as loose or burnt cables must

be eliminated immediately.

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Safety Against Risk of Fire

WARNING

Risk of personal injury or equipment damage. This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol) or materials which could react chemically with sufficient vigor to cause a HAZARD, nor handle or store them within the 30 cm (1ft) clearance envelope surrounding the centrifuge. Do not use the centrifuge in hazardous locations.

Mechanical Safety

For safe operation of the equipment, observe the following:

Safety Notice

Safety Against Risk of Fire

• Use only the rotor and accessories designed for use in this centrifuge.

• Before starting the centrifuge, make sure that the rotor tie-down screw is securely fastened.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop the rotor by hand.

• Do not lift or move the centrifuge while the rotor is spinning.

• If a glass tube breaks inside the chamber bowl, be careful when examining or cleaning the

gasket or chamber, as sharp glass fragments may be embedded in their surfaces.

• NEVER attempt to override the door interlock system while the rotor is spinning.

• Maintain a 30 cm (12 in.) clearance envelope around the centrifuge while it is running. During

operation you should come within the envelope only to adjust instrument controls, if necessary. Never bring any flammable substances within the 30 cm (12 in.) area surrounding the centrifuge. Never lean on the centrifuge or place items on the centrifuge while it is operating.

• Never operate the instrument without a rotor installed.

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Such materials should not be used in this instrument unless all necessary safety precautions are taken.

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• Observe all cautionary information printed on the original solution containers prior to their use.

• Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms. Some of the most virulent— Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi— further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols,

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Safety Notice

Chemical and Biological Safety

observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health and safety guidelines.

• Do not centrifuge materials that could result in a hazardous chemical reaction.

It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter.

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Revision Status,iii

Safety Notice,v

Alerts for Danger, Warning, Caution, Important, and Note,v

Safety During Installation and/or Maintenance,vi

Electrical Safety,vi

Safety Against Risk of Fire,vii

Mechanical Safety,vii

Chemical and Biological Safety,vii

Introduction,xv

Intended Use,xv

Certification,xv

Scope of Manual,xv

Conventions,xvi

Typographic Conventions,xvi

CFC-Free Centrifugation,xvi

Symbols and Labels,xvi

Biohazard,xvi Caution Symbol,xvii High Voltage Danger,xvii Protective Ground,xvii Recycling Label,xvii Risk of Fire Warning,xviii Electric Shock Warning, xviii Rotor Rotation,xviii China RoHS Caution Label,xix Australian EMC Compliance Label,xix CE Mark,xix Consult IFU Label,xx IVD Label,xx TUV-NRTL Label,xx

CHAPTER 1: Description,1-1

Introduction,1-1

Centrifuge Function and Safety Features,1-1

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Contents

Centrifuge Function,1-2 Safety Features,1-2

Chassis,1-3

Housing,1-3 Door,1-3 Rotor Chamber,1-3 Drive,1-4

Controls and Indicators,1-4

Power Switch,1-4 Control Panel,1-5

System Keys,1-5 Program Keys,1-6 Digital Displays,1-7

Name Rating Plate,1-8

Specifications,1-8

Rotor,1-9

CHAPTER 2: Operation,2-1

Introduction,2-1

Run Procedure,2-1

Preparation and Loading,2-2 Entering Run Parameters,2-5

Setting Run Speed,2-5 Setting RPM,2-5 Setting RCF,2-6 Setting Run Time,2-6 Setting Acceleration Rate,2-7

Setting Deceleration Rate,2-7 Starting a Run,2-8 Pulse Function,2-9 Changing Parameters During a Run,2-9 Stopping a Run,2-10

Unloading,2-10

Enabling or Disabling the Audible Tones,2-11

Summary of Run Procedures,2-11

CHAPTER 3: Troubleshooting,3-1

Introduction,3-1

User Messages,3-1

Illustrations

1.1 Front View,1-1

1.2 Back View,1-2

1.3 Interior View of the Rotor Chamber,1-4

1.4 The Power Switch,1-5

1.5 The Control Panel,1-5

1.6 Digital Displays,1-7

2.1 Tapered Sleeve Position,2-2

2.2 Examples of Correctly and Incorrectly Loaded Buckets and Carriers,2-4

2.3 Default Parameters,2-5

3.1 Location of the emergency door release. opening.,3-4

3.2 Insertion of Allen wrench.,3-5

4.1 Locking Screws (Transport Safety Device),4-5

A.1 Locking Screws (Transport Safety Device),A-3 A.2 Dimensions of the Centrifuge,A-4

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Tables

2.1 Acceleration/Deceleration Times (in minutes:seconds),2-7

3.1 Error Message Chart,3-2

3.2 Troubleshooting Chart,3-3

Tables

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Tables

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Intended Use

For In Vitro Diagnostic Use.

This Allegra X-5 centrifuge is intended for the separation of components through the use of relative centrifugal force.

It is designed to separate human samples, including blood and other body fluids, for processing, analysis, and in vitro diagnostic testing, as well as non-human body samples and chemicals, including industrial and environmental samples.

This centrifuge should be operated by qualified personnel only.

Certification

Introduction

The Beckman Coulter Allegra X-5 Centrifuge is manufactured in a facility that maintains certifications to ISO 9001:2008 or ISO 13485:2003. It has been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies. Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com.

Scope of Manual

This manual is designed to familiarize you with the Beckman Coulter Allegra X-5 Centrifuge, its functions, specifications, operation, and routine operator care and maintenance. Beckman Coulter recommends that you read this entire manual, especially the Safety Notice section and all safety related information, before operating the centrifuge or performing instrument maintenance.

The following introductory pages contain the instrument specifications, as well as space, electrical, and temperature conditions required for optimal centrifuge performance. A list of available rotors is also included.

• CHAPTER 1, Description provides a brief physical and functional description of the centrifuge

and the operating controls and indicators.

• CHAPTER 2, Operation contains centrifuge operating procedures.

• CHAPTER 3, Troubleshooting lists diagnostic messages and other possible malfunctions,

together with probable causes and suggested corrective actions.

• CHAPTER 4, Care and Maintenance contains procedures for routine operator care and

maintenance, as well as a brief list of supplies and replacement parts.

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Introduction

Conventions

• APPENDIX A, Installation contains instructions for installing and connecting the centrifuge.

NOTE

If the centrifuge is used in a manner other than specified in this manual, the safety and performance of this equipment could be impaired. Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.

Conventions

Certain symbols are used in the product labeling to call out safety-related and other important information. These international symbols may also be displayed on the centrifuge and are reproduced on the inside of the back cover of this manual.

Typographic Conventions

Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as keys and displays.

• Key names (for example, appear in bold type.

• Cursor keys, used to increment values up or down when setting parameters, are shown as up and down arrows (▲ or ▼).

START

ENTER

) and display names (for example,

TIME

SPEED

)

CFC-Free Centrifugation

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Allegra X-5 centrifuges.

Symbols and Labels

This section provides information for some labels and symbols appearing on the Allegra X-5 instrument housing. These labels and symbols may be associated with user-serviceable procedures. Individual hazards associated with a specific procedure in this manual may use these labels and symbols, and are included in Warnings or Cautions within the procedures for that task.

Biohazard

This caution symbol indicates biohazardous risk from possible patient specimen contamination.

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Caution Symbol

This symbol indicates a caution message and appears adjacent to an explanation or other symbols that define the caution.

High Voltage Danger

This symbol indicates high voltage is present or that there is a risk of electric shock when working in this area. Operation, replacement or servicing of any components where contact with bare, live hazardous parts could occur, possibly resulting in electric shock, should only be performed by your Beckman Coulter representative.

Introduction

Symbols and Labels

Protective Ground

This symbol is used to indicate a protective ground. This instrument must be properly grounded. Do not under any circumstances operate the instrument unless it is properly grounded.

Recycling Label

A28219-AA

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE) Directive of the European Union. The presence of this marking on the product indicates:

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• the device was put on the European Market after August 13, 2005 and

• the device is not to be disposed of via the municipal waste collection system of any member

state of the European Union.

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Introduction

Symbols and Labels

It is very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing this label, please contact your dealer or local Beckman Coulter office for details on the take-back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.

Risk of Fire Warning

Before replacing fuses, shut off power and disconnect the power cord. Failure to do so can cause electric shock and/or equipment damage. Replace fuses only with approved type and rating replacement fuse.

Electric Shock Warning

Operation, replacement or servicing of any components where contact with electronic components could occur can result in electric shock, and should only be performed by your Beckman Coulter representative.

Rotor Rotation

This label indicates the direction of the rotor rotation.

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China RoHS Caution Label

This label and materials declaration table (APPENDIX B, Table of Hazardous Substance’s Name and

Concentration) are to meet People's Republic of China Electronic Industry Standard SJ/T11364-2006

“Marking for Control of Pollution Caused by Electronic Information Products” requirements.

This label indicates that this electronic information product contains certain toxic or hazardous elements, and can be used safely during its environmental protection use period. The number in the middle of the logo is the environmental protection use period for the product.The outer circle indicates that the product can be recycled. The logo also signifies that the product should be recycled immediately after its environmental protection use period has expired. The date on the label indicates the date of manufacture.

Introduction

Symbols and Labels

Australian EMC Compliance Label

The C-Tick mark is intended for use on products that comply with Australian Communication Authority (ACA) EMC Requirements.

CE Mark

A “CE” mark indicates that a product has been assessed before being placed on the market, and has been found to meet European Union safety, health and/or environmental protection requirements.

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Introduction

Symbols and Labels

Consult IFU Label

This label indicates that the Instructions for Use should be referred to for more information.

IVD Label

This label indicates an In Vitro diagnostic medical device.

TUV-NRTL Label

This label indicates recognition by a Nationally Recognized Test Laboratory (NRTL) that the instrument has met the relevant product safety standards.

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Introduction

This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-5 Centrifuge. The operating controls and indicators are also described; instructions for their use are in

CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in

Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.

Centrifuge Function and Safety Features

Figure 1.1 Front View

CHAPTER 1 Description

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1. User Interface

2. Door

3. Main Power Switch

1-1

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Description

Centrifuge Function and Safety Features

Figure 1.2 Back View

1. Main Power Switch

2. Ground Screw

3. Mains Power Input

Centrifuge Function

The Beckman Coulter Allegra X-5 bench top centrifuge generates centrifugal forces required for a variety of applications. Together with the Beckman Coulter rotor designed specifically for use in this centrifuge, the applications include:

• Routine clinical sample processing of serum, plasma, whole blood and other body fluids.

• Rapid sedimentation of protein precipitates, particulates and cells from clinical samples.

• Processing of samples for in-vitro cytotoxicity studies, receptor binding studies and other

specialized tests.

The centrifuges are microprocessor-controlled, providing interactive operation. The instrument design features a brushless three-phase drive system, automatic rotor overspeed identification system, and a choice of acceleration/deceleration rates. User messages and a series of audible tones alert the operator to conditions that may need attention. (Instructions for disabling the audible tones are in CHAPTER 2, Operation.)

Safety Features

The Allegra X-5 Centrifuge has been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft).

Instrument safety features include:

1-2

• An electromechanical door-locking mechanism prevents operator contact with spinning rotors. When the door is closed it locks automatically. It can be unlocked only by pressing the OPEN DOOR key, and opened only when the power is on and the rotor is at rest.

• A steel barrier surrounds the rotor chamber to provide full operator protection.

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Chassis

Housing

Description

Chassis

• An overspeed system continuously monitors the rotor during centrifugation. The system includes a magnetic sensor on the drive motor. Throughout the run, checks are made to ensure that the rotor does not exceed set speed.

• An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor loads are severely out of balance. If the Audible Tones are enabled, a sound signal (beep) is also emitted (see CHAPTER 2, Enabling or Disabling the Audible Tones). At low speeds, an incorrectly loaded rotor can cause imbalance. Rotor instability can also occur if the centrifuge is moved while running, or if it is not resting on a level surface.

• The centrifuge feet, made of rubber, have been designed to minimize possible rotation in the event of a rotor mishap.

The centrifuge housing is made of sheet steel, finished with urethane paint. The control panel is covered by a protective overlay made of coated polycarbonate (Figure 1.1 and Figure 1.2).

Door

The door is made of a solid sheet of steel, encased in foam molding. In the center of the door is a window for strobe viewing. The door is secured to the housing by two hinges. An electromechanical door lock system prevents operator contact with spinning rotors and prevents run initiation unless the door is shut and latched. The door is locked when a run is in progress and can be opened only when the rotor is stopped. (A light-emitting diode [LED] on the door can be opened.) In the event of a power failure, the door lock can be manually released for sample recovery (see CHAPTER 3, Troubleshooting).

Rotor Chamber

The rotor chamber is shown in Figure 1.3. The drive shaft, mounting plate, and a rubber boot surrounding the drive shaft are visible in the chamber bottom. A gasket system around the chamber opening ensures sealing. (Instrument gaskets have not been designed as bioseals for aerosol containment.)

OPEN DOOR

key lights up when the

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Description

Controls and Indicators

Figure 1.3 Interior View of the Rotor Chamber

1. Drive Shaft

2. Tapered Sleeve

Drive

The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation. A tiedown screw is used to attach the rotor to the drive shaft. The resilient suspension ensures that loads are not disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs during centrifugation. Maximum braking may be selected to reduce deceleration time, allowing fast processing of samples; alternately, delicate gradients may be preserved using slower deceleration.

Controls and Indicators

Power Switch

The power switch is located on the centrifuge right side panel (see Figure 1.4). This two-position rocker switch (

, on; O, off) controls electrical power to the centrifuge.

3. Boot

4. Gasket

1-4

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Figure 1.4 The Power Switch

1. Power Switch

Description

Controls and Indicators

NOTE

Control Panel

The control panel is mounted at an angle on the front of the centrifuge and includes system keys, programming keys, and digital displays (see Figure 1.5). The panel also contains an that flashes if rotor loads are severely out of balance.

Figure 1.5 The Control Panel

System Keys

The power must be turned on before the chamber door can be opened.

IMBALANCE

light

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The centrifuge operation is controlled through the system keys. Each key (except the an LED in the upper left corner that lights to indicate that the key can be activated.

PULSE

key) has

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Description

Controls and Indicators

START

STOP The STOP key can be pressed to end a run. It operates in two modes, depending on how

OPEN DOOR

PULSE

Pressing the START key causes the centrifuge run to begin. This key can also be used to abort a deceleration process and restart the centrifuge.

you press it:

• Normal stop (press and release):

The centrifuge decelerates to a complete stop according to the preselected deceleration rate. Deceleration can be terminated and the centrifuge restarted by pressing START again. The centrifuge emits a series of audible tones when the rotor reaches 0 RPM. (Instructions for disabling the tones are in CHAPTER 2, Operation.)

•Fast stop (press and hold for at least two seconds):

The centrifuge decelerates to a complete stop at the maximum rate. The deceleration cannot be interrupted; the centrifuge can only be restarted after the rotor stops and the door is opened and closed.

Pressing the OPEN DOOR key unlatches the centrifuge door locks and allows the door to be opened. The centrifuge accepts this command only when the rotor is completely stopped and the OPEN DOOR key LED is lit.

Pressing the PULSE key causes the installed rotor to accelerate at the maximum rate up to the set speed for short-duration runs (as long as the key is pressed). Deceleration, at the maximum rate, begins when the key is released.

Program Keys

The program keys are used to set run parameters (a program consists of all of the parameters for a

ENTER

run). Except for the cursor and displays, which show the parameters as they are input. Each key (except for the cursor keys) has an LED in the upper left corner that lights to indicate operational readiness. The LEDs also blink if an incorrect parameter is entered.

Cursor Keys

ENTER

RPM

RCF The RCF key can be used to select the speed setting by required relative centrifugal

ROTOR

The cursor keys are up and down arrow keys, which can be pressed to increment values up or down when setting parameters.

Parameter (speed, time, temperature, and acceleration or deceleration profile) changes made while a run is in progress must be verified by pressing the ENTER key.

When the RPM key is pressed the last digit in the RPM/RCF/ROTOR display (0) flashes, indicating that the speed can be entered in increments of 100 revolutions per minute (RPM). After the run starts, the actual RPM of the rotor is displayed.

field (RCF). The corresponding RPM is automatically calculated and displayed during the run. If the RCF key is pressed during the run, the RCF value is shown in the RPM/RCF/ROTOR display.

Pressing the Rotor key displays the rotor number.

keys, program keys are located beneath the applicable digital

1-6

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Description

Controls and Indicators

TIME The TIME key is used to select the run duration. When the TIME key is pressed, the last

digit on the TIME display flashes, indicating that the time can be entered with the cursor keys.

• Timed run — Run time up to 9 hours and 59 minutes can be set. If the minutes

parameter exceeds 59, it is automatically converted into hours.

• Continuous run — If a run time of less than 1 minute or more than 9 hours and

59 minutes is selected, continuous operation is activated, and an infinity symbol (∞) is displayed. Time is not counted down, and the run continues until the STOP key is pressed.

ACCEL The ACCEL key is used to select acceleration rates that protect delicate gradients.

When the ACCEL key is pressed, the ACCEL/DECEL display flashes, indicating that one of ten preset rates can be entered with the cursor keys (9 is the fastest rate and 0 is the slowest rate). Acceleration rates are described in CHAPTER 2,

Acceleration/Deceleration Times (in minutes:seconds).

DECEL The DECEL key is used to select deceleration rates that maintain optimum separation

while protecting delicate gradients. When the DECEL key is pressed, the ACCEL/DECEL display flashes, indicating that one of ten preset rates can be entered with the cursor keys (9 is the fastest rate and 0 is a no-brake coast to stop). Deceleration rate selections are described in CHAPTER 2, Acceleration/Deceleration Times (in

minutes:seconds).

Digital Displays

Digital displays indicate rotor speed, run time, and numbers that represent selected acceleration and deceleration profiles (see Figure 1.6). When the power is turned on, they show the most recently entered parameters, before the power was turned off. The displays serve a dual purpose:

• When the run parameters are being set (the input mode), the displays show the set values (those selected by the operator). When a run parameter key (for example, pressed, the appropriate display flashes to indicate that data can be entered.

• The actual (real-time) operating conditions of the centrifuge are displayed during the run, after

START

is pressed.

NOTE

Figure 1.6 Digital Displays

Error messages (see Table 3.1, Error Message Chart) also appear on the displays, when applicable. The

centrifuge emits a series of audible tones to alert the user to an error condition.

TIME

RPM

) is

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Description

Name Rating Plate

RPM/RCF/ROTOR • In input mode the RPM/RCF/ROTOR display shows the value of the parameter

being set, depending on the programming key pressed (RPM, RCF, or ROTOR). For example, if the ROTOR programming key is pressed, the rotor number appears on the RPM/RCF/ROTOR display.

• During centrifugation, the RPM/RCF/ROTOR display shows the speed of the rotor in RPM. If the RCF key is pressed while the centrifuge is running, the RCF value is displayed.

TIME • During a timed run (between 1 minute and 9 hours, 59 minutes), the TIME

display begins counting down when the rotor starts to spin and continues the countdown until deceleration begins. The TIME display indicates the remaining run time in hours and minutes.

• During a continuous run (less than 1 minute or more than 9 hours, 59 minutes selected), countdown time is not displayed. Instead, the infinity () symbol, indicating continuous operation, lights up and the TIME display shows time elapsed since the run start. After 9 hours and 59 minutes the timer resets to 0 and continues counting elapsed time.

ACCEL/DECEL The ACCEL/DECEL display shows the acceleration profile that was selected for the

run. The deceleration profile number can be displayed by pressing the DECEL key.

Name Rating Plate

The name rating plate is affixed to the right side of the centrifuge. Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge. Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge.

Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee.

Speed

Set speed

Speed control

Speed display

Time

Set time

Time display

100 to 4700 RPM (in 100-RPM increments) actual rotor speed, 50 RPM of set speed actual rotor speed in 100-RPM increments or in RCF (when selected)

1 min. to 9 hr 59 min, (in 1 min. increments), time remaining in run (timed run 1 minute) or  and elapsed time (continuous run)

1-8

Acceleration 10 acceleration rates

Deceleration 10 deceleration rates

Ambient temperature range 5 to 40°C

Humidity restrictions <80% (noncondensing)

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Description

Maximum altitude 2000 m (6562 ft).

Dimensions

Width

Depth

Height, door closed

Height, door open

Weight 77 kg (169 lb)

Clearances (sides) 30 cm (11.8 in.)

Electrical requirements

120-V instrument 200 V instrument

220–240-V instrument

Electrical supply Class I

Maximum heat dissipation into room

under steady-state conditions

Noise level at max. speed (approx.) ≤67 dBa

Installation (overvoltage) category II

Pollution degree

a. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected.

49.6 cm (19.5 in.)

63.4 cm (25.0 in.)

39.0 cm (15.3 in.)

88.0 cm (34.6 in.)

120 VAC, 12A, 60 Hz 200 VAC, 10A, 50/60 Hz 220–240 VAC, 9A, 50/60 Hz

3750 BTU/h (1.1 kW)

Rotor

See the SX4700 User Manual for information on rotor use, care and maintenance, and rotor accessories.

Rotor Profile Description Max RPM Max RCF (x g) Max Capacity

(mL)

SX4700 Swinging Bucket r

= 181 mm

max

4700 4470 100 x 10 mL

140 x 5/7 mL

Rotor Part

Number/

Rotor Manual

Number

B30593/B3034

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Description

Rotor

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Introduction

This section contains operating procedures for the centrifuge, using the Beckman Coulter rotor designed for use in this centrifuge. Refer to the rotor manual for instructions on preparing the rotor for centrifugation.

CHAPTER 2 Operation

NOTE

If the centrifuge is used in a manner other than that specified in this manual, the safety and

performance of this equipment could be impaired.

WARNING

Risk of personal injury or contamination. Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Operator error or tube failure may generate aerosols. Do not run potentially hazardous materials in this centrifuge unless all appropriate safety precautions are taken. Always use the appropriate rotors and adapters.

Handle all infectious samples according to good laboratory practices and methods to prevent the spread of disease. Ask your laboratory safety officer to advise you about the level of containment required for your application and about the proper decontamination or sterilization procedures to follow if fluids escape from containers. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. Because spills may generate aerosols, observe proper safety precautions for aerosol containment.

WARNING

Risk of personal injury or property damage. The centrifuge must not be used in the vicinity of flammable liquids or vapors, and such materials should not be run in the centrifuge. During operation you should come within the 30 cm (12 in.) clearance envelope only to adjust the instrument controls, if necessary. Never bring any flammable substances within the 30 cm (12 in.) area surrounding the centrifuge. Do not lean on the centrifuge or place items on the centrifuge while it is operating.

Run Procedure

The following detailed operating procedures are summarized at the end of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps.

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Operation

Run Procedure

Preparation and Loading

NOTE

Before installing the rotor, lubricate it following the instructions in the rotor manual.

Check the name rating plate for the correct voltage, then plug the power cord into the wall receptacle.

Press the power switch to on (I).

Press the

Use the T-handle wrench to turn the rotor tie-down screw to the left (counterclockwise).

a. Remove the tie-down screw.

Make sure that the tapered sleeve is in place at the base of the centrifuge drive shaft before installing the rotor (see Figure 2.1) and wipe the sleeve to be sure that it is clean and dry.

• The rotor rests on the sleeve while spinning, and does not operate properly if the sleeve is

OPEN DOOR

missing.

key and lift the door up; it remains in the open position.

Figure 2.1 Tapered Sleeve Position

1. Tapered Sleeve

NOTE

If the tapered sleeve comes off, it must be replaced by a Beckman Coulter Field Service

representative. Contact us.

CAUTION

Risk of equipment damage. Do not drop the rotor onto the drive shaft. The shaft can be damaged if the rotor is forced sideways or dropped onto it. Install the rotor by centering it over the shaft and carefully lowering it straight down.

2-2

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Install the rotor according to the instructions in the rotor manual.

a. Lower the rotor straight down onto the drive shaft.

b. Tighten the tie-down screw clockwise onto the drive shaft.

Operation

Run Procedure

c. Seat the filled buckets onto the yoke pins.

• Be sure to fill all positions on the yoke with buckets.

CAUTION

Risk of equipment damage. An improperly loaded rotor can cause severe vibration. Always run the rotor with a balanced load.

Close the centrifuge door and push firmly down on both sides of the door front until you hear a clicking (latching) sound.

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Operation

Run Procedure

Remove the rotor from the centrifuge if a long period between runs is anticipated.

CAUTION

If the rotor is left in the centrifuge between runs, make sure the rotor is seated on the drive shaft and the tie-down screw is tight before each run.

Figure 2.2 Examples of Correctly and Incorrectly Loaded Buckets and Carriers

1. Balanced Load

2. Unbalanced Load

3. Pivotal Axis of Bucket

4. Center of Rotation

NOTE

Contents of opposing buckets must be the same and each bucket must be balanced on its pivotal

5. CORRECT

6. Examples of Symmetrically Loaded Multiwell Plates (load opposite

plates the same way)

7. INCORRECT

8. Examples of Nonsymmetrically Loaded Multiwell Plates

axis.

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Entering Run Parameters

When the power is applied for the first time (no previous runs), default values are displayed (see

Figure 2.3). After the initial use, the parameters of the most recent run are displayed when power is

applied.

Figure 2.3 Default Parameters

1. Accelerate rotor to 2000 RPM.

2. Continue run for two minutes.

3. Use acceleration profile 8.

Operation

Run Procedure

Setting Run Speed

Centrifuge speed can be set for up to the maximum rated speed of the rotor. Either revolutions per minute (RPM) or relative centrifugal field (RCF) can be used to select speed. During centrifugation, the

RPM/RCF/ROTOR

display indicates the actual run speed (RPM) of the rotor.

Setting RPM

Press the

• The last digit on the

RPM

key.

RPM/RCF/ROTOR

entered (in 100-RPM increments) with the cursor keys.

display (0) flashes, indicating that the RPM can be

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Operation

Run Procedure

Press the ▲ or ▼ cursor key until the required RPM is displayed.

• The corresponding RCF is automatically calculated by the centrifuge, but the RPM value is displayed during the run.

RCF

• You can check the RCF during the run by pressing the

running.

key while the centrifuge is

Setting RCF

Press the

RCF

key.

• The last digit on the RCF.

Press the s or t cursor key until the required RCF is displayed.

• The corresponding RPM is automatically calculated and the centrifuge runs at the calculated speed.

RPM/RCF/ROTOR

display (0) flashes, indicating that you can enter the

Setting Run Time

Run time can be set for either a timed run or continuous operation.

Timed Run

Time can be set for up to 9 hours and 59 minutes (if the minutes parameter entered exceeds 59, it is automatically converted into hours). During centrifugation, the when the rotor starts to spin, and continues the count-down until deceleration begins. The display shows the time remaining in the run in hours and minutes. When the time display reaches zero, the run ends.

Continuous Run

If a run time of less than 1 minute or more than 9 hours and 59 minutes is selected, continuous operation is activated. Time is not counted down during continuous operation; instead, the infinity () symbol, indicating continuous operation, lights up and time elapsed from the start of the run is displayed. The run continues until the

STOP

key is pressed.

TIME

display begins counting down

TIME

2-6

Press the

The last digit on the keys.

Press the ▲ or ▼ cursor key until the required run duration is displayed.

TIME

key.

TIME

display flashes, indicating that the time can be entered with the cursor

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Operation

Run Procedure

Setting Acceleration Rate

The

ACCEL

key is used to select acceleration rates that protect delicate gradients. When the

key is pressed, the

ACC/DEC

display flashes, indicating that one of the 10 preset acceleration rates can be entered with the cursor keys. The selected rate depends on the type of run you are performing. For pelleting runs, where sample mixing is not a concern, maximum acceleration (setting 9) can be used. However, if delicate gradients are being run, a lower setting may be needed. Acceleration rates are listed in Table 2.1.

Press the

ACCEL

key.

ACCEL

• The

ACC/DEC

display flashes, indicating that the selected rate number can be entered with

the cursor keys.

Press the ▲ or ▼ cursor key until the required number is displayed.

Tab l e 2 . 1 Acceleration/Deceleration Times (in minutes:secondsa)

4700 RPM

Profile

9 8 7 6

5 4 3

2 1 0

Accel Decel

0:38 0:37 0:45 1:23

2:08 2:47 4:22

8:43 11:37 17:27

0:24 0:32 0:52 1:28

2:11 2:50 4:24

8:44 11:38 coast

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a. Times are approximate; actual

times vary depending on the rotor load, run speed, and voltage fluctuations.

Setting Deceleration Rate

The

DECEL

key is used to select deceleration rates that maintain optimum separation. When the

DECEL

key is pressed, the rates can be entered with the cursor keys. The selected rate depends on the type of run you are performing. For pelleting runs, where sample mixing is not a concern, maximum brake (setting 9) can be used. However, if delicate gradients are being run, a lower brake setting may be needed. Deceleration rates are listed in Table 2.1.

ACC/DEC

display flashes, indicating that one of the ten preset deceleration

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Operation

Run Procedure

Press the

DECEL

key.

The keys.

Press the ▲ or ▼ cursor key until the required number is displayed.

Starting a Run

The run can be started using the parameters in memory from a previous run, or using new or changed parameters that you enter using the procedure described above.

Check that all parameters are correct and the door is shut and latched.

Press the

• The instrument displays the rotor speed as it accelerates, until it reaches and displays the

• If the actual speed exceeds the rotor’s maximum permitted speed of 4700 RPM, an error is

ACC/DEC

speed entered.

displayed and the centrifuge shuts down.

display flashes, indicating that the selected number can be entered with the cursor

ENTER

key, then the

START

key.

— See CHAPTER 3, Troubleshooting for information on error codes. — The error must be cleared and an appropriate speed entered before the centrifuge

can be started.

— Throughout the run, checks are made to ensure that the rotor does not exceed

set speed.

• The

• A blinking LED at the bottom of the

RPM/RCF/ROTOR

— (The RCF can be checked by pressing the

— This display also shows the time remaining in the run (or  and elapsed time for

continuous operation).

display indicates the rotor speed in RPM.

RCF

key.)

TIME

display indicates that the run is in progress.

WARNING

Risk of personal injury. Do not attempt to override the door interlock system while the rotor is spinning or if there is any sound or vibration coming from the drive. Always wait for the rotor to come to a complete stop before attempting to open the centrifuge door.

2-8

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Pulse Function

Operation

Run Procedure

CAUTION

Risk of personal injury or equipment damage. Do not lift or move the centrifuge while the rotor is spinning. Do not place items on the centrifuge during operation.

The pulse function, accessed by pressing the

PULSE

• When the

continues to spin as long as the deceleration settings are overridden by the pulse function.) When the rotor begins decelerating to 0 RPM using maximum deceleration. Using the pulse function eliminates the need to press the

• When the

PULSE

the to show the user-entered settings during a pulse run even though the maximum rates are used.

• If the

• The centrifuge memory retains the parameters of the last run performed before the

PULSE

at speed until the decelerating to 0 RPM using maximum deceleration.

was pressed. At the end of a pulse run, after the centrifuge door is opened and closed the previous run parameters are displayed.

key is pressed, the rotor accelerates at maximum rate to the set speed and

PULSE

key is pressed, the

key is released, the seconds stop accumulating. The

key is pressed while the rotor is running at set speed, the rotor continues running

PULSE

key is released. When the

Changing Parameters During a Run

While a run is in progress, run parameters (speed, time, and acceleration or deceleration rate) can be altered without stopping the run. Run duration can also be changed from continuous to a specified time period, or from a specified time period to continuous.

PULSE

key, is used for short-duration runs.

PULSE

key is pressed. (The current run time, acceleration, and

PULSE

key is released, the

START

and

STOP

keys.

TIME

display begins displaying the elapsed seconds. When

ACCEL/DECEL

PULSE

key is released, the rotor begins

display continues

PULSE

key

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NOTE

Use the program keys as described under Entering Run Parameters (above) to change parameters. Parameter changes made during a run must be verified by pressing the change run speed during centrifugation:

The deceleration rate cannot be changed after deceleration starts.

Press the

• The last digit on the

Press the ▲ or ▼ cursor key until the required RPM is displayed.

RPM

key.

RPM/RCF/ROTOR

or lowered with the cursor keys.

display flashes, indicating that the RPM can be raised

ENTER

key. For example, to

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Operation

Unloading

Press the

• The current RPM value is displayed, changing to the new value as the rotor accelerates or

Stopping a Run

ENTER

key.

decelerates to the new speed selected. — The corresponding RCF is automatically calculated by the centrifuge.

A timed run ends automatically when the series of audible tones when the rotor reaches 0 RPM. (Instructions for disabling the audible tones are on page 2-11, Enabling or Disabling the Audible Tones.) To end a run in progress for any reason:

Unloading

TIME

display counts down to zero. The centrifuge emits a

Press the

(or) Press and hold the

NOTE

After the rotor stops spinning and the release the door latches, then open the door.

STOP

key for normal deceleration as selected by the deceleration profile.

STOP

key for deceleration at the maximum rate (see Table 2.1).

If you hold the STOP key for at least two seconds, the deceleration process cannot be interrupted;

the centrifuge cannot be restarted until the rotor comes to a complete stop and the door is opened and closed.

OPEN DOOR

light comes on, press the

OPEN DOOR

key to

2-10

NOTE

After completing a run, unload the rotor following the instructions in the rotor manual.

When you remove the rotor, make sure that the tapered sleeve from the centrifuge drive shaft does not come out with the rotor. If the tapered sleeve is inside the rotor drive hole, call Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com).

CAUTION

Risk of personal injury or contamination. If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories according to the procedures outlined by your laboratory safety officer.

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Enabling or Disabling the Audible Tones

The audible tones (beeps) that sound at the end of each run and if an error occurs can be turned on and off by following the steps below (rotor must be at 0 RPM).

Press the

Press either the ▲ or ▼ cursor key to turn the beeps on or off.

• (The word “

Press

STOP

key and hold for 3 seconds until the word “

” or “

off

” is displayed to indicate the current setting.

— The arrow keys toggle between on and off settings.)

ENTER

to save the selection.

Operation

Enabling or Disabling the Audible Tones

Beep

” appears in the display.

Summary of Run Procedures

Press the POWER switch to on (I).

a. Open the centrifuge door (press the

Make sure that the tapered sleeve is in place at the base of the centrifuge drive shaft before installing the rotor.

• The rotor does not operate properly if the sleeve is missing.

Install the rotor according to the instructions in the rotor manual.

• Always run the rotor with a balanced load.

Close the centrifuge door and push firmly down on it until you hear the latch engage.

Enter run parameters:

a. Set run speed — b. Set run duration —

RPM

TIME

, ▲ or ▼; or

, ▲ or ▼

OPEN DOOR

RCF

, ▲ or ▼

key and lift the door up).

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c. Select acceleration rate (0 through 9)— d. Select deceleration rate (0 through 9) —

ACCEL

DECEL

, ▲ or ▼ , ▲ or ▼

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Operation

Summary of Run Procedures

Check that all parameters are correct and the door is shut and latched, then press then

START

WARNING

CAUTION

Risk of personal injury or equipment damage. Do not lift or move the centrifuge while the rotor is spinning. Do not place items on the centrifuge during operation.

Wait for the set time to count down to zero, or end the run by pressing or holding the

ENTER

STOP

key.

After the rotor stops spinning and the release the door latch; open the door.

Unload the rotor according to instructions in the rotor manual.

OPEN DOOR

light comes on, press the

OPEN DOOR

CAUTION

key to

2-12

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Introduction

This section lists possible malfunctions, together with probable causes and corrective actions required. Maintenance procedures are contained in CHAPTER 4, Care and Maintenance. For any

problems not covered here, contact us.

CHAPTER 3 Troubleshooting

NOTE

It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before

requesting service by Beckman Coulter.

User Messages

CAUTION

If the message SEr appears on the display, do not press any keys while the message is displayed. Turn the centrifuge power off (O) and back on (I) to clear the message. This message indicates that you have inadvertently accessed the service mode. Pressing any keys while in this mode could erase the centrifuge memory and critically interfere with future operation.

If a problem occurs during operation, the rotor decelerates to a stop, an error code appears on the

RPM/RCF/ROTOR

the error condition. Such problems may result from incorrect input or from an equipment malfunction. Refer to Table 3.1 to determine the nature of the problem and recommended actions. If you are unable to correct the problem, contact us. To help diagnose and correct the problem, gather as much information about the situation as you can:

• Write down the error number that appears on the display.

• Note the operating situation when the error occurred (speed, load type, etc.).

display, and the centrifuge emits a series of audible tones to alert the operator to

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• Note any unusual environmental and/or operating conditions (ambient temperature, voltage fluctuations, etc.).

• Add any other information that may be helpful.

NOTE

Instructions for disabling the audible tones are in CHAPTER 2, Operation.

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Troubleshooting

User Messages

Tab l e 3 . 1 Error Message Chart

Error Number Error Type Recommended Action Note

1-9 System Error • Allow to slow down

• Power off/on

All these errors stop the centrifuge or cause it to decelerate brakeless

10-19 Speedometer error • Allow to slow down

• Power off/on

20-29 Motor error • Power off

• Ensure ventilation

30-39 EEPROM error • Allow to slow down

• Power off/on

With error 34, 35, and 36, the centrifuge will stop; with error 37 and 38 only an error message will be given

46-49 Imbalance error • Allow to slow down

• Power off

• Eliminate the

imbalance

50-59 Door error • Press door key

• Close door

With error 50 and 51, the centrifuge will stop

• Remove foreign

matter from the opening of the door lock device

60-69 Process error • Allow to slow down

• Power off/on

With error 60 message “power failure during run”, with error 61, the message “stop after power on”

3-2

70-79 Communication error • Allow to slow down

• Power off/on

80-89 Parameter error • Power off

• Allow to cool down

• Provide for better

ventilation

90-99 Other errors • Check connections

a. If the recommended action does not correct the problem, contact us.

With error 83, error message only

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Emergency Door Release

If the facility power fails only momentarily, the centrifuge resumes operation when power is restored and the rotor returns to set speed. However, if the rotor comes to a complete stop you have to restart the run when the power is restored. In the event of an extended power failure, you may have to trip the door-locking mechanism manually to remove the rotor and retrieve your sample.

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Troubleshooting

Emergency Door Release

WARNING

Risk of personal injury. Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and/or mechanical injury. Turn the power off and disconnect the instrument from the main power source by removing the Mains (power) plug from the outlet receptacle, and refer such maintenance to qualified service personnel.

Turn the Main power switch to off (O) and disconnect the power cord from the main power source by removing its power plug from the receptacle.

WARNING

Make sure that the rotor is not spinning.

Using a flat blade screwdriver, remove the plug from the opening on the left side of the control panel (Figure 3.1).

Figure 3.1 Location of the emergency door release. opening.

1. Emergency Release access opening

3-4

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Troubleshooting

Emergency Door Release

Insert the supplied 5mm T-handle Allen wrench (P/N B31161) wrench horizontally into the hole (Figure 3.2). The wrench will be guided through a funnel-shaped tube to the shaft of the door

lock motor.

Figure 3.2 Insertion of Allen wrench.

Turn the Allen wrench counter-clockwise to unlock the door.

WARNING

Risk of personal injury. Do not unlock or open the door unless the rotor is at a complete stop.

DANGER

Risk of personal injury. Never try to slow or stop the rotor by hand. Always allow the rotor to come to a complete stop before performing any action inside the rotor chamber.

Remove the Allen wrench and replace the plug.

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Troubleshooting

Emergency Door Release

3-6

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Introduction

For maintenance not covered in this manual, contact us. User messages are discussed in CHAPTER 3,

Troubleshooting. Refer to the rotor manual and to Chemical Resistances (publication IN-175) for

instructions on the care of rotors and their accessories.

CHAPTER 4 Care and Maintenance

NOTE

requesting service by Beckman Coulter Field Service.

Maintenance

It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before

WARNING

Risk of personal injury. Any maintenance procedure or servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury. Make sure that the power switch is off (O) and the centrifuge is disconnected from the main power source by removing the Mains (power) plug from the outlet receptacle, and refer such servicing to qualified service personnel.

CAUTION

Risk of personal injury. Flammability hazard. Do not use Ethanol or other flammable liquids in or near operating centrifuges.

Preventive Maintenance

The following procedures should be performed regularly to ensure continued performance and long service life of the centrifuge.

Regularly inspect the interior of the rotor chamber for accumulations of sample, dust, or glass particles from broken sample tubes.

a. Clean as required (see Cleaning, below), as these accumulations can result in rotor

vibrations.

Regularly check the air intake and exhaust vents for obstructions.

a. Keep vents clear and clean.

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Care and Maintenance

Cleaning

Frequent cleaning is recommended to prolong the life of the centrifuge. Always clean up spills when they occur to prevent corrosives or contaminants from drying on component surfaces.

To prevent the rotor from sticking, lubricate the drive shaft with Spinkote at least once a month, and after each cleaning.

NOTE

Before using any cleaning or decontamination methods, except those recommended by the manufacturer, users should check with the manufacturer that the proposed methods will not damage the equipment.

To prevent accumulations of sample, dust, and/or glass particles from broken sample tubes, keep the interior of the rotor chamber clean and dry by frequent wiping with a cloth or

paper towel.

Clean the drive shaft, shaft cavity, threads, and the tie-down screw at least once a week using a mild detergent such as Solution 555 and a soft brush.

a. Dilute the detergent with water (10 parts water to 1 part detergent). b. Rinse thoroughly and dry completely. c. Lubricate the drive shaft with Spinkote after cleaning.

Wash the bowl using a mild detergent such as diluted Solution 555.

a. Rinse thoroughly and dry completely. b. If a cleaning solution other than Solution 555 is used, consult Chemical Resistances

(publication IN-175) or contact the cleaning-solution vendor to verify that the solution will not damage the centrifuge.

4-2

Clean the centrifuge case and door by wiping with a cloth dampened with diluted Solution 555.

Do not use acetone or other solvents.

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Tube Breakage

If a glass tube breaks, and all the glass is not contained in the bucket or rotor, you must thoroughly clean the interior of the chamber bowl.

WARNING

Risk of personal injury. In the event of tube breakage, sharp glass fragments may be embedded in the surfaces of the sealing gasket or chamber. Be careful when examining or cleaning the sealing gasket or chamber.

Examine the door gasket to make sure that no glass particles are retained in it.

a. Carefully remove any glass particles that may remain.

Carefully wipe away any glass particles that remain in the bowl.

Care and Maintenance

Tube Breakage

Decontamination

If the centrifuge and/or accessories are contaminated with radioactive or pathogenic solutions, perform appropriate decontamination procedures. Refer to Chemical Resistances (IN-175) to be sure the decontamination method will not damage any part of the centrifuge.

Sterilization and Disinfection

The centrifuge is finished with urethane paint. Ethanol (70%) may be used on this surface. See Chemical Resistances for more information regarding chemical resistance of centrifuge and accessory materials.

CAUTION

Risk of personal injury. Flammability hazard. Do not use Ethanol or other flammable liquids in or near operating centrifuges.

While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

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Care and Maintenance

Storage and Transport

Storage

Before storing a centrifuge for an extended period, return it to its original shipping container to pr otec t it f rom d ust an d dirt . Rei nsert the sh ippin g foa m (re moved at time of cen trifu ge in stal lat ion) into the chamber, making sure the drive shaft is stabilized in the hole in the foam.

Temperature and humidity conditions for storage should meet the environmental requirements described in the specifications given in CHAPTER 1, Description.

Notes on Transport

• Install the Transport Safety Device (see Transport Safety Device)

• Always lift the centrifuge with a lifting device

Transport Safety Device

The Transport Safety Device consists of two Allen screws, located on the bottom of the instrument.

Installation

Lift the centrifuge from the front of unit and tilt it backward to expose the bottom of the unit.

Stabilize the centrifuge by placing a suitable object, such as a wooden block under the centrifuge.

Insert the two Allen Screws that were removed during the initial instrument installation (see

APPENDIX A, Transportation Safety Devices) into the holes in the bottom of the centrifuge. Align

the holes in the motor to allow the screws to engage the motor.

Using a #4 Allen wrench, tighten the two Allen screws in a clockwise direction to secure the motor.

4-4

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Figure 4.1 Locking Screws (Transport Safety Device)

1. Locking Screws

Care and Maintenance

Storage and Transport

Returning a Centrifuge

Before returning a centrifuge or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. Contact us to obtain the authorization form and for packaging and shipping instructions.

To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity. Sterilization and decontamination must be done before returning the parts.

All parts must be accompanied by a signed note, plainly visible on the outside of the box, stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach

this notification will result in return or disposal of the items without review of the reported problem.

Install the Transport Safety Device (see Transport Safety Device), and if possible, return in the original packaging.

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Care and Maintenance

Supply List

a partial list is given below.

Refer to the applicable rotor manual for materials and supplies needed for rotors.

Replacement Parts

NOTE

For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.

Description Part Number

Rotor tie-down screw 368245

T handle rotor wrench, size 13 368246

Allen T wrench, size 5 emergency door release

Spinkote 306812

B31161

4-6

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Page 55

Introduction

This chapter contains instructions for installing and connecting the centrifuge. Check that required clearances and electrical power are available.

WARNING

Risk of personal injury or equipment damage. This centrifuge weigh 77kg/169 lbs. DO NOT attempt to lift or move it without assistance from a lifting device and/or another person.

Installing the Instrument

APPENDIX A

Installation

WARNING

Risk of personal injury or equipment damage. Do not place the centrifuge in or near areas containing flammable reagents or combustible fluids. Vapors from these materials could enter the centrifuge air system and be ignited by the motor.

WARNING

Risk of personal injury. Maintain a 30 cm (12 in.) clearance envelope around the centrifuge while it is running. During operation you should come within the 30 cm (12 in.) clearance envelope only to adjust the instrument controls, if necessary. Do not handle or store hazardous materials within the 30 cm (12 in.) area surrounding the centrifuge.

The centrifuge ships in a wooden box on a wooden pallet. For easy access, remove the top of the box, the foam insert on top of the centrifuge, and then the upper part (sides) of the box and set

them aside. a. Then, with the help of another person and/or a lifting device, move the centrifuge from the

pallet to its final position.

• (Note the warning above regarding centrifuge weight.)

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Installation

Transportation Safety Devices

Position the centrifuge on a level surface, such as a sturdy table or laboratory bench that is able to support the weight of the centrifuge and resist vibration.

• Refer to CHAPTER 1, Specifications for weight.

a. Make sure that the centrifuge front feet are fully supported on the table. b. Locate the centrifuge in an area with sufficient ventilation to allow for heat dissipation. c. Check that there are 7.6-cm (3-in.) clearances at the sides of the centrifuge to ensure

sufficient air circulation. Dimensions are shown in Figure A.2. Additional clearance is required on the right side to

allow access to the power switch. The centrifuge must have adequate air ventilation to ensure compliance to local

requirements for vapors produced during operation. Ambient temperatures during operation should not be lower than 5°C (41°F) or higher than

40°C (104°F). Relative humidity should not exceed 80% (non condensing).

NOTE

During transport between areas with varying temperatures, condensation may occur inside

the centrifuge. Allow sufficient drying time before running the centrifuge.

Transportation Safety Devices

A sheet of foam rubber is installed in the rotor chamber at the factory. A hole in the center of the foam stabilizes the drive shaft during transport. On receipt of the centrifuge, remove the foam and store it in case future relocation of the centrifuge is necessary.

Transport Safety Device

The Transport Safety Device consists of two Allen screws, located on the bottom of the instrument. These screws lock the rotor in place and must be removed prior to operating the centrifuge.

Removal

Lift the centrifuge at the front of unit and tilt it backward to expose the bottom of the unit.

Stabilize the centrifuge by placing a suitable object, such as a wooden block, under the centrifuge.

Using a #4 Allen wrench, turn the two Allen screws counter-clockwise to remove them.

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Figure A.1 Locking Screws (Transport Safety Device)

1. Locking Screws

Installation

Electrical Requirements

Save the Transport Safety Device Screws for future transport of the centrifuge.

Electrical Requirements

See the CHAPTER 1, Specifications for centrifuge electrical requirements.

Make sure the voltage and frequency imprinted on the name rating plate affixed to the right side of the centrifuge agree with the line voltage and frequency of the outlet used. Plug in both ends of the centrifuge power cord. If there is any question about voltage, have a qualified service person measure it under load while the drive is operating.

A 1.8-m (6-ft) power cord with grounded plug is supplied with the centrifuge. Make sure that the matching wall outlet is located near the centrifuge and is easily accessible.

NOTE

The power plug serves as the Disconnecting Device and must remain easily accessible.

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Installation

Electrical Requirements

Figure A.2 Dimensions of the Centrifuge

NOTE

Additional clearance is required on the right side to allow to allow access to the power switch.

WARNING

Risk of personal injury. Improper electrical connection could produce an electric shock. To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name rating plate affixed to the centrifuge.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet receptacle strip.

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Test R u n

Installation

Test Run

NOTE

Beckman Coulter recommends that you make a test run to ensure that the centrifuge is in proper operating condition following shipment. See CHAPTER 2, Operation for instructions on operating the centrifuge.

After completing the test run, log on to www.beckmancoulter.com to register your centrifuge. This validates the centrifuge warranty and ensures your receipt of further information regarding new accessories and/or modifications as they become available.

The centrifuge must be plugged in and the power switch turned to on position (I) before the door

can be opened.

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Installation

Test Run

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APPENDIX B

Table of Hazardous Substance’s

Name and Concentration

This materials declaration table (the Table of Hazardous Susbtance’s Name and Concentration) and the China RoHS Caution Label are to meet People's Republic of China Electronic Industry Standard SJ/T11364-2006”Marking for Control of Pollution Caused by Electronic Information Products” requirements.

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B-2

有毒有害物质名称及吉量的标识格式

Table of Hazardous Substance’s Name and Concentration

电子电气产品号码 EEP Part Number: B30585, B30588 产品名称 Product Name: Benchtop Centrifuge

产品型号Product Model Number: Allegra X-5

Table of Hazardous Substance’s Name and Concentration

部件名称

Component Name

印刷电路板组件 Printed Circuit Board Sub Assemblies

电缆组件 Cable Sub Assemblies

塑料部件 Plastic Parts

钣金部件 Sheet Metal

连接部件（螺钉，螺栓等） Hardware (screws, bolts, etc.)

电源组件 Power Supply Sub Assembly

铅

汞

XOOO O O

OOOO O O

XOOO O O

有毒有害物质或元素

Hazardous Substances Name

镉

六价铬

Cr6+

多溴联苯

PBB

多溴二苯醚

PBDE

This table is prepared in accordance with the provisions of SJ/T 11364

O: 表示该有毒有害物质在该部件所有均质材料中的含量均在 GB/T 26572 标准规定的限量要求以下

X: 表示该有毒有害物质至少在该部件的某一均质材料中的含量超出 GB/T 26572 标准规定的限量要求

（企业可在此处，根据实际情况对上表中打“X”的技术原因进行进一步说明）

O: Indicates that the toxic or hazardous substances contained in all of the homogenous materials for this part is below the limit requirements in GB/T 26572.

X: Indicates that this toxic or hazardous substance contained in at least one of the homogenous materials for this part is above the limit requirement in GB/T 26572.

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Beckman Coulter, Inc.

Allegra X-5 Centrifuge Warranty

Subject to the exceptions and upon the conditions specified below, Beckman Coulter agrees to correct, either by repair, or, at its election, by replacement, any defects of material or workmanship which develop within one (1) year after delivery of the Allegra X-5 Centrifuge (the product), to the original Buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use.

Some components and accessories by their nature are not intended to and will not function for as long as one (1) year. A complete list of such components or accessories is maintained at the factory and at each Beckman Coulter District Sales Office. The lists applicable to the products sold hereunder shall be deemed to be part of this warranty. If any such component or accessory fails to give reasonable service for a reasonable period of time, Beckman Coulter will repair or, at its election, replace such component or accessory. What constitutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter.

Replacement

Any product claimed to be defective must, if requested by Beckman Coulter, be returned to the factory, transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.

Conditions

Beckman Coulter shall be released from all obligations under all warranties either expressed or implied, if the product(s) covered hereby are repaired or modified by persons other than its own authorized service personnel, unless such repair in the sole opinion of Beckman Coulter is minor, or unless such modification is merely the installation of a new Beckman Coulter plug-in component for such product(s).

Disclaimer

IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT NEITHER BECKMAN COULTER, INC. NOR ITS SUPPLIERS SHALL HAVE ANY LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.

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Beckman Coulter, Inc. Allegra X-5 Centrifuge Warranty

Warranty-2

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English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어

Symbol Symbol Simbolo Symbole Simbolo

Simbole

символ

符号

記号

상징

Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목

Dangerous voltage Gefährliche electrische Spannung Vol taje pel igroso Courant haute tension Pericolo: alta tensione

Caution, consult accompanying documents Vorsicht, konsultieren Begleitdokumente Atención, consulta documentos adjuntos Attention, consultent des documents d'accompagnement Attenzione, consulta i documenti di accompagnamento

Biohazard Potentiell infektiösem Material Riesgo biológico Risque biologiqu Pericolo biologico

On (power) Ein (Netzverbindung) Encendido Marche (mise sous tension) Acceso (sotto tensione)

Off (power) Aus (Netzverbindung) Apagado Arrêt (mise sous tension) Spento (fuori tensione)

Protective earth (ground) Schutzleiteranschluß Puesta a tierra de protección Liaison à la terre Collegamento di protezione a terra

Earth (ground) Erde (Masse) La tierra (suelo) Te r re (s o l) Scarica a terra

In vitro diagnostic medical device In-vitro-diagnosemedizinisches Gerät Aparato médico de diagnóstico in vitro Appareil médical diagnostique in vitro Apparecchio medico diagnostico in vitro

Manufacturer Hersteller Fab ri ca nt e Fab ri ca nt Fabbricante

Tensão perigosa

Опасное напряжение тока

危险电压危険な電圧 위험한 전압

Cuidado, ulta documentos adjuntos

Внимание, советует с сопроводительными документами

注意, 咨询附属单证注意, 伴う文書に相談しなさい 주의, 동반 문서를 상담하십시오

Material infeccioso potencial биологической опасности

可能的传染性物潜在的な感染性物質 전염하는 물자

Fora (o poder) На (мощности)

开（电源）ン（電源） 에 (힘)

Fora de (poder)

С (сила)

（电源）

ン（電源） 떨어져 (힘)

Te r ra d e pr ot e çã o (t e rr a) Защитное заземление (земля)

保护接地保護アース（接地） 방어적인 지구 (지상)

Te r r a Земли

接地アース（接地） 지구 (지상)

In vitro dispositivo médico diagnóstico В медицинской службе диагностики vitro

体外诊断医疗设备生体外の診断医療機器

생체외 진단 의료 기기

Fabricante

производитель

制造商メーカー 제조자

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English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어

Symbol Symbol Simbolo Symbole Simbolo

Simbole

символ

符号

記号

상징

Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목

Authorized representative in the European Community Autorisierter Repräsentant in der Europäischen Gemeinschaft Representante autorizado en la Comunidad Europea Représentant autorisé dans le Communauté européen Rappresentante autorizzato nella Comunità Europea

Consult Instructions for Use Konsultieren Sie Anwendungsvorschriften Consulte las instrucciones para el uso Consultez les instructions pour l'usage Consulti le istruzioni per uso

Representante autorizado na Comunidade Européia Утверженный представитель в сообществ

在欧共体的授权代表欧州共同体の承認された代表 유럽 공동체에 있는 허가한 대표자

Consulte instruções para o uso Советуйте с инструкциями для пользы

咨询使用说明书使用説明に相談しなさい 사용 설명을 상담하십시오

Symbols-2

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www.beckmancoulter.com

Beckman Coulter Allegra X-5 Instructions For Use Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Instructions For Use

Allegra X-5

Centrifuge

Rx Only in the U.S.A.

Revision Status

Safety Notice

Alerts for Danger, Warning, Caution, Important, and Note

Safety During Installation and/or Maintenance

Electrical Safety

Safety Against Risk of Fire

Mechanical Safety

Chemical and Biological Safety

Contents

Illustrations

Tables

Intended Use

Certification

Introduction

Scope of Manual

Conventions

Typographic Conventions

CFC-Free Centrifugation

Symbols and Labels

Biohazard

Caution Symbol

High Voltage Danger

Protective Ground

Recycling Label

Risk of Fire Warning

Electric Shock Warning

Rotor Rotation

China RoHS Caution Label

Australian EMC Compliance Label

CE Mark

Consult IFU Label

IVD Label

TUV-NRTL Label

Introduction

Centrifuge Function and Safety Features

CHAPTER 1

Description

Centrifuge Function

Safety Features

Chassis

Housing

Door

Rotor Chamber

Controls and Indicators

Drive

Power Switch

Control Panel

System Keys

Program Keys

Digital Displays

Name Rating Plate

Specifications

Rotor

Introduction

CHAPTER 2

Operation

Run Procedure

Preparation and Loading

Entering Run Parameters

Setting Run Speed

Setting RPM

Setting RCF

Setting Run Time

Setting Acceleration Rate

Setting Deceleration Rate

Starting a Run

Pulse Function

Changing Parameters During a Run

Unloading

Stopping a Run

Enabling or Disabling the Audible Tones

Summary of Run Procedures