Beckman Coulter Allegra X-30 Instructions For Use Manual

Instructions For Use

Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Allegra X-30 Series Centrifuges

Rx Only

PN B05529AE
September 2018

Allegra X-30 Series Centrifuges

Beckman Coulter Eurocenter S.A.
22, rue Juste-Olivier
Case Postale 1044
CH - 1260 Nyon 1, Switzerland
Tel: [phone]

PN B05529AE (September 2018)

©2018 Beckman Coulter, Inc.
All rights reserved

Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.

All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.

Contact Us

If you have any questions, contact our Customer Support
Center.

• Worldwide, find us via our website at

www.beckman.com/support/technical

• In the USA and Canada, call us at 1-800-369-0333.

• Outside of the USA and Canada, contact your local

Beckman Coulter Representative.

Glossary of Symbols is available at
beckman.com/techdocs (PN C24689).

Original Instructions

Revision History

For labeling updates, go to www.beckman.com and download the latest version of the manual or system
help for your instrument.

Issue AD, 11/2015

Changes were made to: Introduction, CE Mark.

Issue AE, 09/2018

Changes were made to: CHAPTER 1, Available Rotors.

Note: Changes or additions that are part of the most recent revision are indicated in text by a bar in the
margin of the amended page.

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Revision History

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Safety Notice

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate the instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact us.

Alerts for Danger, Warning, Caution, Important, and Note

DANGER

DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.

IMPORTANT

Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.

NOTE

or servicing of this equipment.

IMPORTANT is used for comments that add value to the step or procedure being performed.

NOTE is used to call attention to notable information that should be followed during installation, use,

Safety During Installation and/or Maintenance

These centrifuges each weigh 48 kg/106 lb (non-refrigerated model) or 78 kg/172 lb (refrigerated
model). DO NOT attempt to lift or move one of them without assistance from another person.

Any servicing of this equipment that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is turned off and the
instrument is disconnected from the main power source by removing the Mains (power) plug from
the outlet receptacle, and refer such servicing to qualified personnel.

Do not replace any centrifuge components with parts not specified for use on this instrument.

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v

Safety Notice

Electrical Safety

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to
connect this equipment to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to
the instrument.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet

receptacle strip.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the instrument and damage electrical or mechanical components.

Safety Against Risk of Fire

This centrifuge is not designed for use with materials capable of developing flammable or explosive
vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol) in this centrifuge nor
handle or store them within the 30-cm (1-ft) clearance envelope surrounding the centrifuge.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the rotors and accessories designed for use in this centrifuge.

• Before starting the centrifuge, make sure that the rotor tie-down screw is securely fastened.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop the rotor by hand.

• Do not lift or move the centrifuge while the rotor is spinning.

• If a glass tube breaks inside the chamber bowl, be careful when examining or cleaning the

gasket or chamber, as sharp glass fragments may be embedded in their surfaces.

• NEVER attempt to override the door interlock system while the rotor is spinning.

• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running. During

operation you should come within the envelope only to adjust instrument controls, if
necessary. Never bring any flammable substances within the 30-cm (1-ft) area surrounding the
centrifuge. Never lean on the centrifuge or place items on the centrifuge while it is operating.

vi

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Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this instrument, however, unless all necessary
safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to
their use.

• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent— Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi —further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.

Safety Notice

Chemical and Biological Safety

It is your responsibility to decontaminate the centrifuge and accessories before requesting service
by Beckman Coulter.

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vii

Safety Notice

Chemical and Biological Safety

viii

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Contents

Revision History,iii

Safety Notice,v

Alerts for Danger, Warning, Caution, Important, and Note,v

Safety During Installation and/or Maintenance,v

Electrical Safety,vi

Safety Against Risk of Fire,vi

Mechanical Safety,vi

Chemical and Biological Safety,vii

Introduction,xv

Intended Use,xv

Certification,xv

Scope of Manual,xv

Conventions,xvi

CFC-Free Centrifugation,xvi

Recycling Label,xvi

CE Mark,xvii

CHAPTER 1: Description,1-1

Introduction,1-1

Centrifuge Function and Safety Features,1-1

Centrifuge Function,1-1
Models,1-1
Safety Features,1-2

Chassis,1-2

Housing,1-2
Door,1-2
Rotor Chamber,1-2
Drive,1-3

Temperature Sensing and Control (refrigerated models only),1-3

Controls and Indicators,1-4

Power Switch,1-4
Control Panel,1-4

ix

Contents

Name Rating Plate,1-8

Nonrefrigerated Model Specifications,1-8

Refrigerated Model Specifications,1-9

Available Rotors,1-10

CHAPTER 2: Operation,2-1

Introduction,2-1

Run Procedure,2-2

Preparation and Loading,2-2
Entering Run Parameters,2-6
Starting a Run,2-10
Pulse Function,2-11
Changing Parameters During a Run,2-11
Stopping a Run,2-12

Unloading,2-12

Disabling the Audible Tones,2-13

Summary of Run Procedures,2-13

CHAPTER 3: Troubleshooting,3-1

Introduction,3-1

User Messages,3-1

Other Possible Problems,3-3

Emergency Access,3-4

CHAPTER 4: Care and Maintenance,4-1

Introduction,4-1

Maintenance,4-1

Preventive Maintenance,4-1

Cleaning,4-2

Tube Breakage,4-3

Decontamination,4-3

Sterilization and Disinfection,4-3

x

Storage and Transport,4-4

Storage,4-4
Returning a Centrifuge,4-4

Supply List,4-4

Replacement Parts,4-4
Other,4-4

APPENDIX A: Installation,A-1

Introduction,A-1

Installing the Instrument,A-1

Transportation Safety Devices,A-2

Electrical Requirements,A-2

Test Run,A-4

Beckman Coulter, Inc.
Allegra X-30 Series Centrifuge Warranty

Contents

xi

Illustrations

Illustrations

1.1 Interior View of the Rotor Chamber,1-3

1.2 The Power Switch,1-4

1.3 System Panels,1-4

1.4 System Keys,1-5

1.5 Program Keys,1-6

1.6 Digital Displays,1-7

2.1 Tapered Sleeve Position,2-3

2.2 Default Parameters,2-6

3.1 Screw Locations,3-4

3.2 Internal Access,3-5
A.1 Dimensions of the Refrigerated and Nonrefrigerated

Centrifuges,A-3

xii

Tables

2.1 Acceleration/Deceleration Times (in minutes:seconds),2-9

3.1 Error Message Chart,3-2

3.2 Troubleshooting Chart,3-3

Tables

xiii

Tables

xiv

Intended Use

For In Vitro Diagnostic Use

This Allegra X-30 centrifuge is intended for the separation of components through the use of
relative centrifugal force.

It is designed to separate human samples, including blood and other body fluids, for processing,
analysis, and in vitro diagnostic testing, as well as nonhuman body samples and chemicals, including
industrial and environmental samples.

This centrifuge should be operated by qualified personnel only.

Certification

Introduction

Beckman Coulter Allegra X-30 Series centrifuges are manufactured in a facility that maintains
certifications to both ISO 9001:2008 and ISO 13485:2003. They have been designed and tested to be
compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements
of applicable regulatory agencies. Declarations of conformity and certificates of compliance are
available at www.beckmancoulter.com.

Scope of Manual

This manual is designed to familiarize you with the Beckman Coulter Allegra X-30 Series
centrifuges, its functions, specifications, operation, and routine operator care and maintenance. We
recommend that you read this entire manual, especially the Safety Notice section and all safety­related information, before operating the centrifuge or performing instrument maintenance.

• The following introductory pages contain the instrument specifications, as well as space,
electrical, and temperature conditions required for optimal centrifuge performance. A list of
available rotors is also included.

• CHAPTER 1, Description provides a brief physical and functional description of the centrifuge,

the operating controls and indicators, and system specifications.

• CHAPTER 2, Operation contains centrifuge operating procedures.

• CHAPTER 3, Troubleshooting lists diagnostic messages and other possible malfunctions,

together with probable causes and suggested corrective actions.

• CHAPTER 4, Care and Maintenance contains procedures for routine operator care and

maintenance, as well as a brief list of supplies and replacement parts.

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xv

Introduction

A28219-AA

Conventions

• APPENDIX A, Installation contains instructions for installing and connecting the centrifuge.

NOTE

If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.

Conventions

Certain symbols are used in the product labeling to call out safety-related and other important
information. These international symbols may also be displayed on the centrifuge and are
reproduced on the inside of the back cover of this manual.

Typographic Conventions

Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.

• Key names (for example,
appear in bold type.

• Cursor keys, used to increment values up or down when setting parameters, are shown as up and
down arrows (▲ or ▼).

START

or

ENTER

) and display names (for example,

TEMP°C

or

SPEED

)

CFC-Free Centrifugation

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of
Allegra X-30 Series centrifuges.

Recycling Label

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:

1. the device was put on the European market after August 13, 2005 and

2. the device is not to be disposed via the municipal waste collection system of any member state

of the European Union.

xvi

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CE Mark

Introduction

CE Mark

It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.

A “CE” mark indicates that a product has been assessed before being placed on the market, and has
been found to meet European Union safety, health, and/or environmental protection
requirements.

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xvii

Introduction

CE Mark

xviii

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Introduction

This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-30
Series centrifuges. The operating controls and indicators are also described; instructions for their use are
in CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in
Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.

Centrifuge Function and Safety Features

Centrifuge Function

CHAPTER 1

Description

Models

The Beckman Coulter Allegra X-30 Series benchtop centrifuges generate centrifugal forces required
for a wide variety of applications. Together with any of several Beckman Coulter rotors designed
specifically for use in these centrifuges, the instrument applications include:

• Routine processing such as sample preparations, pelleting, extractions, purifications,
concentrations, phase separations, receptor binding, and column centrifugations.

• Processing large numbers of small-volume samples in multi-well plates for concentrating
tissue-culture cells, cloning and replicate studies, in-vitro cytoxicity studies, receptor binding,
and genetic engineering experimentation.

• Rapid sedimentation of protein precipitates, large particles, and cell debris.

• Binding studies and separation of whole blood.

• Cell isolation.

The centrifuges are microprocessor-controlled, providing interactive operation. The instrument
design features a brushless three-phase drive system, automatic rotor overspeed identification
system, and a choice of acceleration/deceleration rates. The refrigerated models also have
temperature control systems. User messages and a series of audible tones alert the operator to
conditions that may need attention. (Instructions for disabling the audible tones are in CHAPTER 2,

Operation.)

The centrifuge is available in both non-refrigerated and refrigerated models. See Nonrefrigerated
Model Specifications or Refrigerated Model Specifications for the operating differences between the

two models. Unless indicated otherwise, information in this manual is the same for both models.

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1-1

Description

Chassis

Safety Features

Allegra X-30 Series centrifuges have been designed and tested to operate safely indoors at altitudes
up to 2000 m (6562 ft).

Instrument safety features include:

• The door has an electromechanical door-locking mechanism to prevent operator contact with
spinning rotors. When the door is closed it locks automatically. It can be unlocked only by
pressing the
Two independent monitoring systems prevent the door from opening if the rotor is spinning.

• A steel barrier surrounds the rotor chamber to provide full operator protection.

• An overspeed system continuously monitors the rotor during centrifugation. The system

includes a magnetic sensor on the drive motor and magnets embedded in the rotors.
Throughout the run, checks are made to ensure that the rotor does not exceed set speed.

• An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor
loads are severely out of balance. At low speeds, an incorrectly loaded rotor can cause
imbalance. Rotor instability can also occur if the centrifuge is moved while running, or if it is
not resting on a level surface.

• The centrifuge feet, made of rubber, have been designed to minimize possible rotation in the
event of a rotor mishap.

OPEN DOOR

key, and opened only when the power is on and the rotor is at rest.

Chassis

Housing

The centrifuge housing is made of sheet steel, finished with urethane paint. The control panel is
covered by a protective overlay made of coated polycarbonate.

Door

The door is made of a solid sheet of steel, encased in foam molding. In the center of the door is a
window for strobe viewing. The door is secured to the housing by solid shafts. An electromechanical
door lock s ystem prevents oper ator co ntact with spinning rotors and prevents run initiation unless
the door is shut and latched. The door is locked when a run is in progress and can be opened only
when the rotor is stopped. (A light-emitting diode [LED] on the
door can be opened.) In the event of a power failure, the door lock can be manually released for
sample recovery (see CHAPTER 3, Troubleshooting).

Rotor Chamber

The rotor chamber is shown in Figure 1.1. The drive shaft, mounting plate, rubber boot surrounding
the drive shaft, thermistor, and rotor detector are visible in the chamber bottom. A gasket system
around the chamber opening ensures sealing. (Instrument gaskets have not been designed as
bioseals for aerosol containment.)

OPEN DOOR

key lights up when the

1-2

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Figure 1.1 Interior View of the Rotor Chamber

Description

Temperature Sensing and Control (refrigerated models only)

1

Drive

6

5

4

1. Drive Shaft

2. Tapered Sleeve

3. Boot

1

2

3

4. Gasket

5. Rotor Detector

6. Thermistor (refrigerated models only)

The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation. A tie­down screw is used to attach the rotor to the drive shaft. The resilient suspension ensures that loads
are not disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs
during centrifugation. Maximum braking may be selected to reduce deceleration time, allowing fast
processing of samples; alternately, delicate gradients may be preserved using slower deceleration.

Temperature Sensing and Control (refrigerated models only)

With the power on, the temperature control system is activated when the door is closed. The run
temperature can be set between –20 and +40°C in refrigerated models. If no set temperature is
entered, the centrifuge automatically selects the last entered temperature. (For the first run of a
new centrifuge, the instrument selects 20°C as its operating temperature.) A thermistor in the rotor
chamber continuously monitors chamber temperature. The micro-processor calculates the
required chamber temperature to maintain the selected rotor temperature.

NOTE

In the unlikely event of a complete cooling system failure, the drive switches off if the chamber

temperature goes above 50°C. Restarting the centrifuge is not possible until the chamber is cooled.

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1-3

Description

1

Controls and Indicators

Controls and Indicators

Power Switch

The power switch is located on the centrifuge right side panel (see Figure 1.2). This two-position
rocker switch (

Figure 1.2 The Power Switch

I

, on; O, off) controls electrical power to the centrifuge.

1. Power Switch

NOTE

Control Panel

The control panel is mounted at an angle on the front of the centrifuge and includes system keys,
programming keys, and digital displays (see Figure 1.3). The panel also contains an
that flashes if rotor loads are severely out of balance.

Figure 1.3 System Panels

The power must be turned on before the chamber door can be opened.

IMBALANCE

light

1-4

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Description

Controls and Indicators

System Keys

The centrifuge operation is controlled through the system keys (see Figure 1.4). Each key (except

PULSE

the
activated.

Figure 1.4 System Keys

START Pressing the START key causes the centrifuge run to begin. This key can also be used

STOP The STOP key can be pressed to end a run. It operates in two modes, depending on

key) has an LED in the upper left corner that lights to indicate that the key can be

to abort a deceleration process and restart the centrifuge.

how you press it:

• Normal stop (press and release):
The centrifuge decelerates to a complete stop according to the preselected

deceleration curve. Deceleration can be terminated and the centrifuge restarted by
pressing START again. The centrifuge emits a series of audible tones when the
rotor reaches 0 rpm. (Instructions for disabling the tones are in CHAPTER 2,

Operation.)

• Fast stop (press and hold for at least two seconds):
The centrifuge decelerates to a complete stop at the maximum rate. The

deceleration cannot be interrupted; the centrifuge can only be restarted after the
rotor stops and the door is opened and closed.

1

OPEN DOOR Pressing the OPEN DOOR key unlatches the centrifuge door locks and allows the door

to be opened. The centrifuge accepts this command only when the rotor is completely
stopped and the OPEN DOOR key LED is lit.

PULSE Pressing the PULSE key causes the installed rotor to accelerate at the maximum rate

up to the set speed for short-duration runs (as long as the key is pressed).
Deceleration, at the maximum rate, begins when the key is released.

Program Keys

The program keys (see Figure 1.5) are used to set run parameters (a program consists of all of the

ENTER

parameters for a run). Except for the cursor and
applicable digital displays, which show the parameters as they are input. Each key (except for the
cursor keys) has an LED in the upper left corner that lights to indicate operational readiness. The
LEDs also blink if an incorrect parameter is entered.

keys, program keys are located beneath the

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1-5

Description

Controls and Indicators

Figure 1.5 Program Keys

▲ ▼

(cursor keys)

The cursor keys are up and down arrow keys (▲ and ▼), which can be pressed to
increment values up or down when setting parameters.

ENTER Parameter (speed, time, temperature, and acceleration or deceleration curve)

changes made while a run is in progress must be verified by pressing the ENTER key.

RPM When the RPM key is pressed the last digit in the SPEED display (0) flashes, indicating

that the speed can be entered in increments of 100 revolutions per minute (rpm).
After the run starts, the actual rpm of the rotor is displayed.

RCF The RCF key can be used to select the speed setting by required relative centrifugal

field (RCF). The corresponding rpm is automatically calculated and displayed during
the run. If the RCF key is pressed during the run, the RCF value is shown in the SPEED
display.

ROTOR The centrifuge memory contains a list of the rotors that can be used, together with

default parameters for each rotor. When the ROTOR key is pressed the number of the
rotor used in the previous run is shown on the SPEED display. The rotor list can be
scrolled through, using the cursor keys, until the required rotor number appears.

TIME The TIME key is used to select the run duration. When the TIME key is pressed, the

last digit on the TIME display flashes, indicating that the time can be entered with the
cursor keys.

• Timed run — Run time up to 9 hours and 59 minutes can be set. If the minutes
parameter exceeds 59, it is automatically converted into hours.

• Continuous run — If a run time of less than 1 minute or more than 9 hours and
59 minutes is selected, continuous operation is activated. Time is not counted
down, and the run continues until the STOP key is pressed.

TEMP

(refrigerated
models only)

The TEMP key is used to select run temperature on refrigerated model centrifuges.
When the TEMP key is pressed, the TEMP°C display flashes, indicating that the
temperature can be entered with the cursor keys. Temperature can be set between
–20 and +40°C. The operating temperature range is +2°C to +40°C, depending on
the rotor used and the speed selected.

ACCEL The ACCEL key is used to select acceleration rates that protect delicate gradients.

When the ACCEL key is pressed, the ACC/DEC
ten preset rates can be entered with the cursor keys (9 is the fastest rate and 0 is the
slowest rate). Acceleration rates are described in Table 2.1 in CHAPTER 2, Operation.

DECEL The DECEL key is used to select deceleration rates that maintain optimum separation

while protecting delicate gradients. When the DECEL key is pressed, the ACC/DEC
display flashes, indicating that one of ten preset rates can be entered with the cursor
keys (9 is the fastest rate and 0 is a no-brake coast to stop). Deceleration rate
selections are described in Table 2.1 in CHAPTER 2, Operation.

1-6

display flashes, indicating that one of

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Description

Controls and Indicators

Digital Displays

Digital displays indicate rotor speed, run time, rotor chamber temperature, and numbers that
represent selected acceleration and deceleration profiles (see Figure 1.6). When the power is turned
on, they show the operating parameters of the most recent run performed before the power was
turned off. The displays serve a dual purpose.

• When the run parameters are being set (the input mode), the displays show the set values
(those selected by the operator). When a run- parameter key (for example,
pressed, the appropriate display flashes to indicate that data can be entered.

• The actual (real-time) operating conditions of the centrifuge are displayed during the run, after

START

is pressed.

TIME

or

RPM

) is

1

NOTE

Figure 1.6 Digital Displays

Error messages (see Section 4) also appear on the displays, when applicable. The centrifuge emits a

series of audible tones to alert the user to an error condition.

RPM/RCF/ROTOR • In input mode the RPM/RCF/ROTOR display shows the value of the parameter

being set, depending on the programming key pressed (RPM, RCF, or ROTOR).
For example, if the ROTOR programming key is pressed, a rotor number
appears on the SPEED display.

• During centrifugation, the SPEED display shows the speed of the rotor in rpm.
If the RCF key is pressed while the centrifuge is running, the RCF value is
displayed.

TIME • During a timed run (between 1 minute and 9 hours, 59 minutes), the TIME

display begins counting down when the rotor starts to spin and continues the
countdown until deceleration begins. The TIME display indicates the remaining
run time in hours and minutes.

• During a continuous run (less than 1 minute or more than 9 hours, 59 minutes
selected), countdown time is not displayed. Instead, the infinity () symbol,
indicating continuous operation, lights up and the TIME display shows time
elapsed since the run start. After 9 hours and 59 minutes the timer resets to 0
and continues counting elapsed time.

TEMP °C

(refrigerated
models only)

ACCEL/DECEL The ACCEL/DECEL display shows the acceleration curve that was selected for the

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During standby (that is, the centrifuge is turned on but not spinning) and operation,
the TEMP °C display shows the actual temperature inside the rotor chamber ( 2°C
at an ambient temperature of 20°C).

run. The deceleration curve number can be displayed by pressing the DECEL key.

1-7

Description

Name Rating Plate

Name Rating Plate

The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with
the voltage listed on this name rating plate before connecting the centrifuge. Always mention the
serial number and the model number shown when corresponding with Beckman Coulter regarding
your centrifuge.

Nonrefrigerated Model Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.

Speed

Set speed
Speed control
Speed display

Time

Set time
Time display

Acceleration 10 acceleration profiles

Deceleration 10 deceleration profiles

Ambient temperature range 4 to 35°C

Humidity restrictions <80% (noncondensing)

Dimensions

Width
Depth
Height, door closed
Height, door open

Weight 48 kg (106 lb)

Clearances (sides) 7.6 cm (3.0 in.)

to 16,000 rpm (in 100-rpm increments)
actual rotor speed, 50 rpm of set speed
actual rotor speed in 100-rpm increments or in RCF (when selected)

to 9 hr 59 min or continuous ()
time remaining in run (timed run 1 minute) or  and elapsed time
(continuous run)

46 cm (18.1 in.)
55 cm (21.7 in.)

35.5 cm (14.0 in.)

78.7 cm (31.0 in.)

Electrical requirements

120-V instrument
100-V instrument
220–240-V instrument

Electrical supply Class I

Maximum heat dissipation into room
under steady-state conditions

1-8

120 VAC, 6 A, 60 Hz
100 VAC, 7 A, 50–60 Hz
220–240 VAC, 2.6 A, 50–60 Hz

1638 Btu/h (0.48 kW)

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Description

Refrigerated Model Specifications

1

Noise level 0.91 m (3 ft) in front of
instrument (approx.)

Installation (overvoltage) category II

Pollution degree

a. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected.

£68 dBa

a

2

Refrigerated Model Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.

Speed

Set speed
Speed control
Speed display

Time

Set time
Time display

Temperature

Set temperature
Temperature control (after equilibration)
Temperature display (after equilibration)
Operating range
Ambient temperature range

Acceleration 10 acceleration profiles

Deceleration 10 deceleration profiles

Ambient temperature range 10 to 35°C

Humidity restrictions <80% (noncondensing)

Dimensions

Width
Depth
Height, door closed
Height, door open

Weight 78 kg (172 lb)

Clearances (sides) 7.6 cm (3.0 in.)

Electrical requirements

120-V instrument
100-V instrument
220–240-V instrument

to 18,000 rpm (in 100-rpm increments)
actual rotor speed, 50 rpm of set speed
actual rotor speed in 100-rpm increments or in RCF (when selected)

to 9 hr 59 min or continuous ()
time remaining in run (timed run 1 min accuracy) or  and elapsed
time (continuous run)

–20 to +40°C (in 1°C increments)
2.5°C of set temperature
chamber temperature in 1°C increments

2 to 40°C
10 to 35°C

46 cm (18.1 in.)

70.7 cm (27.8 in.)
37 cm (14.6 in.)

81.3 cm (32.0 in.)

120 VAC, 11.5 A, 60 Hz
100 VAC, 12.6 A, 50–60 Hz
220–240 VAC, 6.2 A, 50–60 Hz

a

PN B05529AE

1-9

Description

Available Rotors

Electrical supply Class I

Maximum heat dissipation into room under

3311 Btu/h (0.97 kW)

steady-state conditions

Noise level 0.91 m (3 ft) in front of

£68 dBa

instrument (approx.)

Installation (overvoltage) category II

Pollution degree

a. Temperature range depends on rotor in use and speed (see applicable rotor manual).

b. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected.

b

2

Available Rotors

See the applicable rotor manual for information on rotor use, care and maintenance, and rotor
accessories.

Rotor Profile Description Refrigerated Nonrefrigerated Max

Capacity
Max
RPM

Max RCF
( g)

Max
RPM

Max
RCF

(mL)

( g)

F2402H

a

18,000 29,756 16,000 23,511 24  1.5/2.0 361171

Fixed Angle
45° Angle

r

= 82 mm

max

FX301.5

16,000

b

28,672

b

13,200 19,515 30  1.5/2.0 392274
Fixed Angle
45° Angle

r

= 100 mm

max

F0630

18,000 28,305 16,000 22,364 6  30 361231
Fixed Angle
30° Angle

r

= 78 mm

max

F0850

11,400 13,682 9000 8528 8  50 346640
Fixed Angle
25° Angle

r

= 94 mm

max

Rotor Part
Number/
Rotor Manual
Number

GS-TB-021

MMR-TB-002

GS-TB-014

GS-TB-003

F0685
Fixed Angle
25° Angle
r

= 97 mm

max

1-10

10,000 10,864 8000 6953 6  85 364650

GS-TB-008

PN B05529AE

Description

Available Rotors

1

Rotor Profile Description Refrigerated Nonrefrigerated Max

Capacity
Max
RPM

Max RCF
( g)

Max
RPM

Max
RCF

(mL)

( g)

F1010

18,000 27,579 16,000 21,791 10  10 361221

Fixed Angle
35° Angle
r

= 76 mm

max

C0650 (Conical)

10,000 10,304 9000 8346 6  50 364670

Fixed Angle
25° Angle

r

= 92 mm

max

C1015 (Conical)

10,000 10,416 9000 8437 10 15 364680

Fixed Angle
25° Angle

r

= 93 mm

max

S2096
Microtiter

3000 1109 3000 1109 6 ea. 96  0.3 mL

2 ea. 96  2 mL

rmax = 110 mm

Rotor Part
Number/
Rotor Manual
Number

GS-TB-007

GS-TB-009

GS-TB-011

361111
GS-TB-005

S6096
Microtiter
rmax = 110 mm

SX4250

4700 2721 4700 2721 6 ea. 96  0.3 mL

2 ea. 96  2 mL

4500 3901 4200 3398 4  250 392243

Swinging
Bucket

r

= 172 mm

max

SX4400

a

4700 4255 4200 3398 4  400 B01425

Swinging
Bucket

r

= 172 mm

max

SX241.5

14,000 16,244 12,500 12,950 24  1.5/2.0 392271

Swinging
Bucket

r

= 74 mm

max

a. Certified for biocontainment by Porton Down U.K.

b. Minimum achievable temperature at full rated speed is 8°C. To achieve a lower temperature the speed must be reduced.

B01430

GS22-TB-002

MMR-TB-003

PN B05529AE

1-11

Description

Available Rotors

1-12

PN B05529AE

Introduction

This section contains operating procedures for the centrifuge, using any of the Beckman Coulter rotors
designed for use in these centrifuges. Refer to the applicable rotor manual for instructions on preparing
the rotor for centrifugation. To prevent condensation, keep the centrifuge door closed and the power
turned off (

O

) when the centrifuge is not in use.

CHAPTER 2

Operation

NOTE

If the centrifuge is used in a manner other than that specified in this manual, the safety and

performance of this equipment could be impaired.

PN B05529AE

2-1

Operation

Run Procedure

WARNING

Normal operation may involve the use of solutions and test samples that are
pathogenic, toxic, or radioactive. Operator error or tube failure may generate
aerosols. Do not run potentially hazardous materials in this centrifuge unless all
appropriate safety precautions are taken. Always use the appropriate rotors and
adapters.

Handle all infectious samples according to good laboratory practices and methods
to prevent the spread of disease. Ask your laboratory safety officer to advise you
about the level of containment required for your application and about the proper
decontamination or sterilization procedures to follow if fluids escape from
containers. Biosafe containment should be used when Risk Group II materials (as
identified in the World Health Organization Laboratory Biosafety Manual) are
handled; materials of a higher group require more than one level of protection.
Because spills may generate aerosols, observe proper safety precautions
for aerosol containment.

WARNING

The centrifuge must not be used in the vicinity of flammable liquids or vapors, and
such materials should not be run in the centrifuge. During operation you should
come within the 7.6 cm (3-in.) clearance envelope only to adjust instrument
controls, if necessary. Never bring any flammable substances within the 30-cm
(1-ft) area surrounding the centrifuge. Do not lean on the centrifuge or place
items on the centrifuge while it is operating.

Run Procedure

The following detailed operating procedures are summarized at the end of this section. If you
are an experienced user of this centrifuge, you can turn to the summary for a quick review of
operating steps.

Preparation and Loading

For fast temperature equilibration, cool or warm the rotor to the required temperature before the run.

NOTE

For high-speed runs at temperatures of 20°C or higher, prime the refrigeration system (refrigerated

models) by running the instrument at 10°C for 5 to 10 minutes beforehand to prevent overheating.

NOTE

1

2-2

Before installing the rotor, lubricate it following the instructions in the rotor manual.

Check the name rating plate for the correct voltage, then plug the power cord into the wall
receptacle.

PN B05529AE

Operation

Run Procedure

2

Press the power switch to on (I).

3

Press the

4

Use the T-handle wrench to turn the rotor tie-down screw to the left (counterclockwise).

a. Remove the tie-down screw.

5

Make sure that the tapered sleeve is in place at the base of the centrifuge drive shaft before
installing the rotor (see Figure 2.1) and wipe the sleeve to be sure that it is clean and dry.

• The rotor rests on the sleeve while spinning, and does not operate properly if the sleeve is

Figure 2.1 Tapered Sleeve Position

OPEN DOOR

missing.

key and lift the door up; it remains in the open position.

2

1

1. Tapered Sleeve

NOTE

If the tapered sleeve comes off, it must be replaced by a Beckman Coulter Field Service
representative. Call Beckman Coulter Field Service (1-800-742-2345 in the United States; outside
the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com).

CAUTION

Do not drop the rotor onto the drive shaft. The shaft can be damaged if the rotor
is forced sideways or dropped onto it. Install the rotor by centering it over the
shaft and carefully lowering it straight down.

6

Install the rotor according to the instructions in the applicable rotor manual.

For a swinging bucket rotor, the steps include the following:

PN B05529AE

2-3

Operation

Run Procedure

a. Lower the yoke straight down onto the drive shaft.

b. Tighten the tie-down screw clockwise onto the drive shaft.

c. Seat the filled buckets onto the yoke pins.

• Be sure to fill all positions on the yoke with buckets.

For a fixed angle rotor, the steps include the following:

2-4

PN B05529AE

a. Lower the rotor straight down onto the drive shaft.

b. Tighten the tie-down screw clockwise onto the drive shaft.

Operation

Run Procedure

2

c. Attach the lid and tighten it. Some rotors have a knob and others have a bolt for the

T-handle wrench.

NOTE

Fixed-angle rotors can be centrifuged without lids attached when longer tubes are used.

PN B05529AE

2-5

Operation

Run Procedure

CAUTION

Always run any type of rotor with a balanced load.

7

Close the centrifuge door and push firmly down on both sides of the door front until you hear
a clicking (latching) sound.

8

Remove the rotor from the centrifuge if a long period between runs is anticipated.

CAUTION

If the rotor is left in the centrifuge between runs, make sure the rotor is seated on
the drive shaft and the tie-down screw is tight before each run.

Entering Run Parameters

When the power is applied for the initial use (no previous runs), default values are displayed (see

Figure 2.2). After the initial use, the parameters of the latest previous run are displayed when power

is applied.

When run parameters for a rotor have been entered, as described below, they are retained in the
centrifuge memory and can be recalled by simply entering the rotor number. The recalled program
can then be used for the current run or altered as required.

Figure 2.2 Default Parameters

1. Accelerate rotor to 2000 RPM.

2. Continue run for two minutes.

3. Cool chamber to 20°C (refrigerated models only).

4. Use acceleration curve 8.

2-6

PN B05529AE

Operation

Run Procedure

Selecting a Rotor Number

Each rotor that can be run in the centrifuge has a rotor number; the number is engraved on the
rotor (and on the lid of fixed angle rotors). The centrifuge internal memory contains a list of all
rotors that can be run in the centrifuge, together with acceptable run parameters for each rotor.
If you enter an unauthorized rotor number then press the
and the run is aborted shortly after the rotor starts spinning.

1

Press the

A rotor number (the last rotor previously centrifuged) appears on the

2

Press the ▲ or ▼ cursor key until the number of the rotor in use appears.

3

Press

• Nominal parameter values (time, temperature, speed, acceleration and deceleration

• You can use these parameters or set new values for the run.

ROTOR

key.

ENTER

.

curves) for the selected rotor are shown.

START

key, an error message is displayed

SPEED

display.

2

Setting Run Speed

Centrifuge speed can be set for up to the maximum rated speed of the selected rotor. Either
revolutions per minute (rpm) or relative centrifugal field (RCF) can be used to select speed. During
centrifugation, the

SPEED

display indicates the actual run speed (RPM) of the rotor.

Setting RPM

1

Press the

• The last digit on the

2

Press the ▲ or ▼ cursor key until the required RPM is displayed.

• The corresponding RCF is automatically calculated by the centrifuge, but the RPM value is

• You can check the RCF during the run by pressing the

RPM

key.

SPEED

display (0) flashes, indicating that the RPM can be entered (in

100-RPM increments) with the cursor keys.

displayed during the run.

running.

RCF

key while the centrifuge is

PN B05529AE

2-7

Operation

Run Procedure

Setting RCF

1

Press the

• The last digit on the

2

Press the ▲ or ▼ cursor key until the required RCF is displayed.

• The corresponding RPM is automatically calculated and the centrifuge runs at the

RCF

key.

calculated speed.

SPEED

display (0) flashes, indicating that you can enter the RCF.

Setting Run Time

Run time can be set for either a timed run or continuous operation.

Timed Run

Time can be set for up to 9 hours and 59 minutes (if the minutes parameter entered exceeds 59, it is

TIME

automatically converted into hours). During centrifugation, the
when the rotor starts to spin and continues the count-down until deceleration begins. The
display shows the time remaining in the run, in hours and minutes. When the time display reaches
zero, the run ends.

display begins counting down

TIME

Continuous Run

If a run time of less than 1 minute or more than 9 hours and 59 minutes is selected, continuous
operation is activated. Time is not counted down during continuous operation; instead, the infinity
() symbol, indicating continuous operation, lights up and time elapsed since the run start is
displayed. The run continues until the

1

Press the

The last digit on the
cursor keys.

2

Press the ▲ or ▼ cursor key until the required run duration is displayed.

TIME

key.

TIME

display flashes, indicating that the time can be entered with the

STOP

key is pressed.

Setting Run Temperature (refrigerated models only)

Run temperature can be set between –20 and +40°C. The typical operating range is from +2°C to
40°C, depending on the rotor and speed selected.

NOTE

Temperatures may vary slightly between instruments. If sample temperature is crucial, test

temperature settings on your instrument using water samples.

1

Press the

2-8

TEMP

key.

PN B05529AE

The

TEMP°C

display flashes, indicating that the temperature can be entered with the

cursor keys.

2

Press the ▲ or ▼ cursor key until the required run temperature is displayed.

Operation

Run Procedure

2

NOTE

For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast
equilibration. For low-temperature runs, precool the centrifuge by running a 30-minute cycle at the
required temperature (with a precooled rotor installed) with the speed set at about 2000 rpm.

Setting Acceleration Rate

The

ACCEL

key is used to select acceleration rates that protect delicate gradients. When the

key is pressed, the

ACC/DEC

display flashes, indicating that one of the 10 preset acceleration rates
can be entered with the cursor keys. The selected rate depends on the type of run you are
performing. For pelleting runs, where sample mixing is not a concern, maximum acceleration
(setting 9) can be used. However, if delicate gradients are being run, a lower setting may be needed.
Acceleration rates are listed in Table 2.1.

1

Press the

• The

ACCEL

ACC/DEC

key.

display flashes, indicating that the selected rate number can be entered with

the cursor keys.

2

Press the ▲ or ▼ cursor key until the required number is displayed.

ACCEL

Table 2.1 Acceleration/Deceleration Times (in minutes:secondsa)

Curve Refrigerated Models Nonrefrigerated Models

Swinging Bucket
Rotors

(4 700 rpm)

Accel Decel Accel Decel Accel Decel Accel Decel

9
8
7
6

5
4
3

2
1
0

PN B05529AE

0:30
0:36
0:53
1:38

2:22
3:06
4:52

9:51
13:10
19:37

b

0:24
0:35
0:55
1:38

2:12
3:10
4:55

9:46

13:02
coast

Fixed Angle Rotors
(18,000 rpm)

0:53
1:36
3:03
6:04

9:07
12:00
18:44

37:29
50:03

c

74:56

0:46
1:33
3:03
6:02

9:07
12:01
18:45

37:29
49:58

coast

Swinging Bucket
Rotors

(4 200 rpm)

0:38
0:37
0:45
1:23

2:08
2:47
4:22

8:43

11:37

c

17:27

b

0:24
0:32
0:52
1:28

2:11
2:50
4:24

8:44

11:38
coast

Fixed Angle Rotors
(16,000 rpm)

0:44
1:24
2:45
5:24

8:05
10:39

16:38

33:17
44:24

c

66:37

0:43
1:24
2:43
5:21

8:07
10:40

16:40

33:19
44:25

coast

c

2-9

Operation

Run Procedure

a. Times are approximate; actual times vary depending on the rotor in use, the rotor load, run speed, and voltage fluctuations.

b. Maximum speed for the S2096 microtiter rotor is 3000 rpm; acceleration and deceleration times decrease accordingly.

c. Deceleration setting 0 is a no-brake coast to stop and, in some cases, may take less time than deceleration setting 1.

Setting Deceleration Rate

The

DECEL

key is used to select deceleration rates that maintain optimum separation. When the

DECEL

key is pressed, the
rates can be entered with the cursor keys. The selected rate depends on the type of run you are
performing. For pelleting runs, where sample mixing is not a concern, maximum brake (setting 9)
can be used. However, if delicate gradients are being run, a lower brake setting may be needed.
Deceleration rates are listed in Table 2.1.

1

Press the

DECEL

key.

ACC/DEC

display flashes, indicating that one of the ten preset deceleration

The
cursor keys.

2

Press the ▲ or ▼ cursor key until the required number is displayed.

Starting a Run

The run can be started using the parameters in memory from a previous run, or using new or
changed parameters that you enter using the procedure described above.

1

Check that all parameters are correct and the door is shut and latched.

2

Press the

• As the run begins, the instrument rotor identification system compares the rotor in the

• An incorrect rotor identification, or set speed greater than the rotor’s maximum

ACC/DEC

display flashes, indicating that the selected number can be entered with the

ENTER

key, then the

START

key.

chamber to the entered rotor number and the speed entered.

permitted speed, results in an error code and the centrifuge shuts down.

— See CHAPTER 3, Troubleshooting for information on error codes.
— The error must be cleared and an appropriate speed entered before the centrifuge

can be started.

— Throughout the run, checks are made to ensure that the rotor does not exceed

set speed.

• The

SPEED

display indicates the rotor speed in rpm.

— (The RCF can be checked by pressing the

2-10

RCF

key.)

PN B05529AE

Operation

Run Procedure

2

• A blinking LED at the bottom of the

Pulse Function

The pulse function, accessed by pressing the

• When the
continues to spin as long as the
deceleration settings are overridden by the pulse function.) When the
the rotor begins decelerating to 0 rpm using maximum deceleration. Using the pulse function
eliminates the need to press the

TIME

display indicates that the run is in progress.

— This display also shows the time remaining in the run (or  and elapsed time for

continuous operation).

WARNING

Do not attempt to override the door interlock system while the rotor is spinning.

CAUTION

Do not lift or move the centrifuge while the rotor is spinning. Do not place items
on the centrifuge during operation.

PULSE

key, is used for short-duration runs as follows.

PULSE

key is pressed, the rotor accelerates at maximum rate to the set speed and

PULSE

key is pressed. (The current run time, acceleration, and

PULSE

key is released,

START

and

STOP

keys.

• When the
the

PULSE

key is pressed, the

PULSE

key is released, the seconds stop accumulating. The

to show the user-entered settings during a pulse run even though the maximum rates are used.

• If the

PULSE

key is pressed while the rotor is running at set speed, the rotor continues running

at speed until the

PULSE

key is released. When the

decelerating to 0 rpm using maximum deceleration.

• The centrifuge memory retains the parameters of the last run performed before the
was pressed. At the end of a pulse run, after the centrifuge door is opened and closed the
previous run parameters are displayed.

Changing Parameters During a Run

While a run is in progress, run parameters (speed, time, temperature, and acceleration or
deceleration rate) can be altered without stopping the run. Run duration can also be changed from
continuous to a specified time period, or from a specified time period to continuous.

NOTE

Use the program keys as described under Entering Run Parameters, above, to change parameters.
Parameter changes made during a run must be verified by pressing the
change run speed during centrifugation:

The deceleration rate cannot be changed after deceleration starts.

TIME

display begins displaying the elapsed seconds. When

ACCEL/DECEL

PULSE

key is released, the rotor begins

ENTER

display continues

key. For example, to

PULSE

key

PN B05529AE

2-11

Operation

Unloading

1

Press the

• The last digit on the

2

Press the ▲ or ▼ cursor key until the required rpm is displayed.

3

Press the

• The current RPM value is displayed, changing to the new value as the rotor accelerates or

Stopping a Run

RPM

key.

SPEED

display flashes, indicating that the rpm can be raised or lowered

with the cursor keys.

ENTER

key.

decelerates to the new speed selected.
— The corresponding RCF is automatically calculated by the centrifuge.

A timed run ends automatically when the
series of audible tones when the rotor reaches 0 rpm. (Instructions for disabling the audible tones
are on page 2-13, Disabling the Audible Tones.) To end a run in progress for any reason:

1

Press the

(or)
Press and hold the

NOTE

2

After the rotor stops spinning and the
release the door latches, then open the door.

NOTE

STOP

key for normal deceleration as selected by the deceleration curve.

STOP

key for deceleration at the maximum rate (see Table 2.1).

If you hold the STOP key for more than a full second, the deceleration process cannot be
interrupted; the centrifuge cannot be restarted until the rotor comes to a complete stop and the door
is opened and closed.

To prevent chamber icing, use a sponge to wipe condensation out of the chamber bowl
between runs.

TIME

display counts down to zero. The centrifuge emits a

OPEN DOOR

light comes on, press the

OPEN DOOR

key to

Unloading

NOTE

2-12

When you remove the rotor, make sure that the tapered sleeve from the centrifuge drive shaft
does not come out with the rotor. If the tapered sleeve is inside the rotor drive hole, call Beckman Coulter
Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter
office or visit us at www.beckmancoulter.com).

PN B05529AE

After completing a run, unload the rotor following the instructions in the applicable rotor manual.

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply appropriate decontamination procedures to the
centrifuge and accessories.

Disabling the Audible Tones

The audible tones (beeps) that sound at the end of each run and if an error occurs may be turned on
and off by following the steps below (rotor must be at 0 rpm).

1

Press the

STOP

key and hold for 3 seconds until the word “

Disabling the Audible Tones

Beep

” appears in the display.

Operation

2

2

Press either the ▲ or ▼cursor key to turn the beeps on or off.

• (The word “
— The arrow keys toggle between on and off settings.)

3

Press

ENTER

on

” or “

off

to save the selection.

Summary of Run Procedures

For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration.
For low-temperature runs, precool the centrifuge by running a 30-minute cycle (with a precooled rotor
installed) at the required temperature with the speed set at 2000 rpm.

1

Press the POWER switch to on (I).

a. Open the centrifuge door (press the

2

Make sure that the tapered sleeve is in place at the base of the centrifuge drive shaft before
installing the rotor.

” is displayed to indicate the current setting.

OPEN DOOR

key and lift the door up).

• The rotor does not operate properly if the sleeve is missing.

3

Install the rotor according to the instructions in the applicable rotor manual.

• Always run the rotor with a balanced load.

PN B05529AE

2-13

Operation

Summary of Run Procedures

4

Close the centrifuge door and push firmly down on it until you hear the latch engage.

5

Enter run parameters:

a. Select a rotor number —

ROTOR

, ▲ or ▼,

ENTER

b. Set run speed —
c. Set run duration —
d. Set run temperature —
e. Select acceleration rate (0 through 9)—
f. Select deceleration rate (0 through 9)—

6

Check that all parameters are correct and the door is shut and latched, then press
then

START

.

RPM

, ▲ or ▼; or

TIME

, ▲ or ▼

TEMP

, ▲ or ▼

RCF

, ▲ or ▼

ACCEL

DECEL

, ▲ or ▼
, ▲ or ▼

WARNING

Never attempt to override the door interlock system while the rotor is spinning.

CAUTION

Do not lift or move the centrifuge while the rotor is spinning.

7

Wait for the set time to count down to zero, or end the run by pressing or holding the

ENTER

STOP

,

key.

8

After the rotor stops spinning and the
release the door latch; open the door.

9

Unload the rotor according to instructions in the applicable rotor manual.

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply appropriate decontamination procedures applied to the
centrifuge and accessories.

2-14

OPEN DOOR

light comes on, press the

OPEN DOOR

PN B05529AE

key to

Introduction

This section lists possible malfunctions, together with probable causes and corrective actions
required. Maintenance procedures are contained in CHAPTER 4, Care and Maintenance. For any

problems not covered here contact Beckman Coulter Field Service.

CHAPTER 3

Troubleshooting

NOTE

It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before

requesting service by Beckman Coulter.

User Messages

CAUTION

If the message SEr appears on the display, do not press any keys while the
message is displayed. Turn the centrifuge power off (O) and back on (I) to clear
the message. This message indicates that you have inadvertently accessed the
service mode. Pressing any keys while in this mode could erase the centrifuge
memory and critically interfere with future operation.

If a problem occurs during operation, the rotor decelerates to a stop, an error code appears on the

SPEED

display, and the centrifuge emits a series of audible tones to alert the operator to the error
condition. Such problems may result from incorrect input or from an equipment malfunction. Refer
to Table 3.1 to determine the nature of the problem and recommended actions. If you are unable to
correct the problem, call Beckman Coulter Field Service. To help diagnose and correct the problem,
gather as much information about the situation as you can:

• Write down the error number that appears on the display.

• Note the operating situation when the error occurred (rotor in use, speed, load type, etc.).

• Note any unusual environmental and/or operating conditions (ambient temperature, voltage

fluctuations, etc.).

• Add any other information that may be helpful.

NOTE

PN B05529AE

Instructions for disabling the audible tones are in CHAPTER 2, Operation.

3-1

Troubleshooting

User Messages

Table 3.1 Error Message Chart

a

Error Number Problem Result Recommended Action

1 through 23,
25 and 26,
28 through 30

Microprocessor or
mechanical
malfunction

Deceleration to full
stop; run cannot be
restarted

After rotor comes to a complete stop, turn
the power off (O), then back on (I) to reset.

32, 36, 39 and 40,
44 through 60,
63 through 68

23 and 24, 27, 31,
33 through 35,
37 and 38,

Microprocessor or
mechanical
malfunction

Deceleration to full
stop; run cannot be
restarted

After rotor comes to a complete stop, open
and close the centrifuge door, then restart.

41 through 43, 62

69 through 77 Microprocessor

malfunction

78 through 80 Error during closing of

door

Run cannot start until
error is cleared

Turn the power off (O), then back on (I) to
reset.

Run cannot start 1. Remove debris in latch

2. Close lid quickly.

3. Turn the power off (O), then back on (I)

to reset.

81 “Door open” detected

during run

Maximum deceleration
to full stop

After rotor comes to a complete stop, shut
the centrifuge door, turn the power off (O),
then back on (I) to reset.

82, 83 Door does not open — See Emergency Access, below

84 Motor over

temperature

85 through 87 Rotor chamber over

temperature
(refrigerated models)

Deceleration to full stop 1. Check that ambient temperature is

within a the limits shown in

Deceleration to full stop

Specifications in CHAPTER 1.

2. Check air inlets and exhausts for
obstructions.

After the motor has cooled, restart. If the
problem persists, call Beckman Coulter Field

b

90 through 96 Temperature sensor

malfunction

Maximum deceleration
to full stop

Service.

Turn the power off (O), then back on (I) to
reset.

(refrigerated models)

98 Rotor not recognized Maximum deceleration

to full stop

Check that the installed rotor is usable in the
centrifuge. If incorrect, install an authorized
rotor; if rotor is authorized and problem
persists, call Beckman Coulter Field Service.

99 Rotor recognized but

incorrect

a. If the recommended action does not correct the problem, call Beckman Coulter Field Service.

b. In the United States, call 1-800-742-2345; outside the U.S., contact your local Beckman Coulter office or visit us at www.beckmancoulter.com.

Maximum deceleration
to full stop

Input correct parameters for the rotor in use,
then restart.

3-2

PN B05529AE

Other Possible Problems

Possible malfunctions that may not be indicated by diagnostic messages are described in Table 3.2,
along with probable causes and corrective actions required. Possible causes for each problem are
listed in the probable order of occurrence. Perform the recommended corrective action in
sequence, as listed. If you are unable to correct the problem, call Beckman Coulter Field Service.

Table 3.2 Troubleshooting Chart

Problem Problem/Result Recommended Action

Troubleshooting

Other Possible Problems

3

Imbalance LED lights
and rotor decelerates
to stop

Rotor cannot achieve
set speed

Door does not open 1. Rotor spinning

Displays are blank 1. Power not on

1. Rotor is out of balance

2. Centrifuge is misaligned (tilted)

3. Centrifuge was moved during

operation

4. Drive error (mechanical damage)

1. Line voltage below rating

2. Electrical failure

3. Motor failure

2. Power not on

3. Source power failure

4. Latch stuck

2. Electrical failure

3. Fuse blown

1. Check to be sure the rotor is in good condition and

is loaded symmetrically around the center of
rotation, with containers of equal weight and
density opposite each other.

2. Align the centrifuge on the bench or table.

3. After the rotor comes to a complete stop, open and

close the centrifuge door, then restart.

4. Call Beckman Coulter Field Service.

1. Have a qualified service person measure line

voltage while the instrument is operating.

2. Make sure both ends of the power cord are
securely connected; call Beckman Coulter Field
Service.

3. Call Beckman Coulter Field Service.

1. Wait until the rotor stops.

2. Plug in the power cord; turn power on (I).

3. See Emergency Access, below.

4. See Emergency Access below.

1. Plug in the power cord; turn power on (I).

2. Make sure both ends of the power cord are

securely connected; call Beckman Coulter Field
Service.

3. System fuses cannot be replaced by the user. Call
Beckman Coulter Field Service.

TEMP °C display
flashes (refrigerated
models)

Chamber does not
reach selected
temperature
(refrigerated models)

PN B05529AE

Chamber temperature is >25°C
higher than selected temperature

Centrifuge cannot maintain selected
temperature for rotor in use at speed
selected

Precool rotors before running at low temperatures.
Precool rotor chamber by running a 30-minute cycle at
the desired temperature with the speed set at about
2000 rpm. If a lower temperature deviation alert is
required, contact Beckman Coulter Field Service.

Refer to applicable rotor manual for temperature and
speed requirements. Also, precool rotors before
running at low temperatures. Precool rotor chamber by
running a 30-minute cycle at the desired temperature
with the speed set at 2000 rpm.

3-3

Troubleshooting

Emergency Access

Emergency Access

If the facility power fails only momentarily, the centrifuge resumes operation when power is
restored and the rotor returns to set speed. However, if the rotor comes to a complete stop you have
to restart the run when the power is restored. In the event of an extended power failure, you may
have to trip the door-locking mechanism manually to remove the rotor and retrieve your sample.

WARNING

Any maintenance procedure requiring removal of a panel exposes the operator to
the possibility of electrical shock and/or mechanical injury. Therefore, turn the
power off and disconnect the instrument from the main power source by removing
its power plug from the receptacle, and refer such maintenance to qualified
service personnel.

1

Turn the power switch to off (O) and disconnect the power cord from the main power source
by removing its power plug from the receptacle.

WARNING

Never attempt to override the door interlock system while the rotor is spinning.

2

Make sure that the rotor is not spinning.

CAUTION

Do not proceed if there is any sound or vibration coming from the drive.

3

Use a small flat-blade screwdriver to pry off the two round screw-hole covers on the centrifuge
front panel (see Figure 3.1).

Figure 3.1 Screw Locations

1. Upper screws and covers. 2. Lower screws, not visible from the front.

3-4

PN B05529AE

Troubleshooting

Emergency Access

4

Set the covers aside.

5

Insert a Phillips-head screwdriver through one of the exposed holes in the centrifuge front
panel and loosen, but do not remove, the screw.

a. Repeat for the other screw.

6

Move the centrifuge forward on the bench until the two screws on the bottom of the centrifuge
are accessible (see Figure 3.1).

7

Use a Phillips-head screwdriver to remove the two screws and set them aside.

8

Carefully lift the centrifuge front cover until the bottom releases, then pull the top towards you
slightly.

To support the cover, insert the cover bottom lip under the standoff plate inside the centrifuge.

3

CAUTION

Do not allow the cover to hang down supported only by the exposed power and
grounding wires or the wires will be damaged.

9

Pull the latch-release cable firmly to the right until the latches release and the door opens
(see Figure 3.2).

Figure 3.2 Internal Access

1. Latch release cable.

2. Standoff plate.

3. Cover bottom lip goes here.

10

If the rotor is still spinning, close the door and wait until it stops before attempting to remove it.

PN B05529AE

3-5

Troubleshooting

Emergency Access

11

DANGER

Never try to slow or stop the rotor by hand.

After removing the rotor, replace the front panel, screws, and covers.

3-6

PN B05529AE

Introduction

For maintenance not covered in this manual, contact Beckman Coulter Field Service. User messages are
discussed in CHAPTER 3, Troubleshooting. Refer to the applicable rotor manual and to Chemical
Resistances (publication IN-175) for instructions on the care of rotors and their accessories.

CHAPTER 4

Care and Maintenance

NOTE

It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before

requesting service by Beckman Coulter Field Service.

WARNING

Any maintenance procedure or servicing of this equipment that requires removal
of any covers can expose parts which involve the risk of electric shock or personal
injury. Make sure that the power switch is off (O) and the centrifuge is
disconnected from the main power source by removing its power plug from the
receptacle, and refer such servicing to qualified service personnel.

Do not use alcohol or other flammable substances in or near operating
centrifuges.

Maintenance

Preventive Maintenance

The following procedures should be performed regularly to ensure continued performance and
long service life of the centrifuge.

1

Regularly inspect the interior of the rotor chamber for accumulations of sample, dust, or glass
particles from broken sample tubes.

a. Clean as required (see Cleaning, below), as these accumulations can result in rotor

vibrations.

2

Regularly check the air intake and exhaust vents for obstructions.

a. Keep vents clear and clean.

PN B05529AE

4-1

Care and Maintenance

Cleaning

3

4

Cleaning

Frequent cleaning is recommended to prolong the life of the centrifuge. Always clean up spills when
they occur to prevent corrosives or contaminants from drying on component surfaces.

Use a sponge to wipe condensation out of the chamber bowl between runs to prevent chamber
icing (refrigerated models).

a. If chamber icing occurs, defrost before use.

To prevent the rotor from sticking, lubricate the drive shaft with Spinkote at least once a
month, and after each cleaning.

NOTE

1

2

3

Before using any cleaning or decontamination methods except those recommended by the
manufacturer, users should check with the manufacturer that the proposed methods will not damage the
equipment.

To prevent accumulations of sample, dust, and/or glass particles from broken sample tubes,
keep the interior of the rotor chamber clean and dry by frequent wiping with a cloth or

paper towel.

Clean the drive shaft, shaft cavity, threads, and the tie-down screw at least once a week using a
mild detergent such as Beckman Solution 555 and a soft brush.

a. Dilute the detergent 10 to 1 with water.
b. Rinse thoroughly and dry completely.
c. Lubricate the drive shaft with Spinkote after cleaning.

Wash the bowl using a mild detergent such as diluted Solution 555.

a. Rinse thoroughly and dry completely.
b. If a cleaning solution other than Solution 555 is used, consult Chemical Resistances

(publication IN-175) or contact the cleaning-solution vendor to verify that the solution
will not damage the centrifuge.

4

Clean the centrifuge case and door by wiping with a cloth dampened with diluted Solution 555.

Do not use acetone or other solvents.

4-2

PN B05529AE

Tube Breakage

If a glass tube breaks, and all the glass is not contained in the bucket or rotor, you must thoroughly
clean the interior of the chamber bowl.

WARNING

Be careful when examining or cleaning the sealing gasket or chamber, as sharp
glass fragments may be embedded in their surfaces.

1

Examine the gasket to make sure that no glass particles are retained in it.

a. Carefully remove any glass particles that may remain.

2

Carefully wipe away any glass particles that remain in the bowl.

Care and Maintenance

Tube Breakage

4

Decontamination

If the centrifuge and/or accessories are contaminated with radioactive or pathogenic solutions,
perform appropriate decontamination procedures. Refer to Chemical Resistances (IN-175) to be sure
the decontamination method will not damage any part of the centrifuge.

Sterilization and Disinfection

The centrifuge is finished with urethane paint. Ethanol (70%)* may be used on this surface. See
Chemical Resistances for more information regarding chemical resistance of centrifuge and
accessory materials.

While Beckman Coulter has tested these methods and found that they do not damage the
centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or
disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

* Flammability hazard. Do not use in or near operating centrifuges.

PN B05529AE

4-3

Care and Maintenance

Storage and Transport

Storage and Transport

Storage

Before storing a centrifuge for an extended period, return it to its original shipping container
to protect it from dust and dirt. Reinsert the shipping foam (removed at time of centrifuge
installation) into the chamber, making sure the drive shaft is stabilized in the hole in the foam.
Temperature and humidity conditions for storage should meet the environmental requirements
described in the specifications given in CHAPTER 1, Description.

Returning a Centrifuge

Before returning a centrifuge or accessory for any reason, prior permission must be obtained from
Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the form and for
packaging and shipping instructions.

To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from
pathogens and/or radioactivity. Sterilization and decontamination must be done before returning
the parts.

All parts must be accompanied by a signed note, plainly visible on the outside of the box, stating that they
are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach

this notification will result in return or disposal of the items without review of the reported problem.

Supply List

Contact Beckman Coulter Sales or visit www.beckman.com for information about ordering parts,
supplies, and publications. For your convenience, a partial list is given below.

Refer to the applicable rotor manual for materials and supplies needed for rotors.

Replacement Parts

Description Part Number

Rotor tie-down screw 361367

T-handle wrench 361371

Other

NOTE

4-4

For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.

PN B05529AE

Description Part Number

Silicone vacuum grease (1 oz) 335148

Beckman Solution 555 (1 qt) 339555

Care and Maintenance

Supply List

4

PN B05529AE

4-5

Care and Maintenance

Supply List

4-6

PN B05529AE

Introduction

This chapter contains instructions for installing and connecting the centrifuge. Check that required
clearances and electrical power are available.

WARNING

These centrifuges each weigh 48 kg/106 lb (non-refrigerated model) or 78
kg/172 lb (refrigerated model.) DO NOT attempt to lift or move one of them
without assistance from a lifting device or another person.

Installing the Instrument

APPENDIX A

Installation

WARNING

Do not place the centrifuge near areas containing flammable reagents or
combustible fluids. Vapors from these materials could enter the centrifuge air
system and be ignited by the motor.

WARNING

Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it
is running. No persons should be within this clearance boundary while the
centrifuge is operating. Do not handle or store hazardous materials within the
30-cm (1-ft) area surrounding the centrifuge.

1

The centrifuge ships in a cardboard box on a wooden pallet. For easy access, remove the top of
the box, the foam insert on top of the centrifuge, and then the upper part (sides) of the box and

set them aside.
a. Then, with the help of another person, move the centrifuge from the pallet to its final

position.

• (Note the warning above regarding centrifuge weight.)

2

Position the centrifuge on a level surface, such as a sturdy table or laboratory bench that is able
to support the weight of the centrifuge and resist vibration.

• Refer to Nonrefrigerated Model Specifications or Refrigerated Model Specifications in

CHAPTER 1 for weight.

PN B05529AE

A-1

Installation

Transportation Safety Devices

a. Make sure that the centrifuge front feet are fully supported on the table.
b. Locate the centrifuge in an area with sufficient ventilation to allow for heat dissipation.
c. Check that there are 7.6-cm (3-in.) clearances at the sides of the centrifuge to ensure

sufficient air circulation.
Dimensions are shown in Figure A.1. Additional clearance is required on the right side to

allow access to the power switch.
The centrifuge must have adequate air ventilation to ensure compliance to local

requirements for vapors produced during operation.
Ambient temperatures during operation should not be lower than 10°C (50°F) or higher

than 35°C (95°F) for refrigerated models, or lower than 4°C (39.2°F) or higher than 35°C
(95°F) for non-refrigerated models. Relative humidity should not exceed 80%
(noncondensing).

NOTE

During transport between areas with varying temperatures, condensation may occur inside

the centrifuge. Allow sufficient drying time before running the centrifuge.

Transportation Safety Devices

A sheet of foam rubber is installed in the rotor chamber at the factory. A hole in the center of the
foam stabilizes the drive shaft during transport. On receipt of the centrifuge, remove the foam and
store it in case future relocation of the centrifuge is necessary.

Electrical Requirements

See the Nonrefrigerated Model Specifications or Refrigerated Model Specifications in CHAPTER 1 for
centrifuge electrical requirements.

Make sure the voltage and frequency imprinted on the name rating plate affixed to the back of the
centrifuge agree with the line voltage and frequency of the outlet used. Refrigeration does not
function properly if the frequency [Hz] does not match the name rating plate. Plug in both ends of
the centrifuge power cord. If there is any question about voltage, have a qualified service person
measure it under load while the drive is operating.

A 1.8-m (6-ft) power cord with grounded plug is supplied with the centrifuge. Make sure that the
matching wall outlet is located near the centrifuge and is easily accessible.

NOTE

The power plug serves as the Disconnecting Device and must remain easily accessible.

A-2

PN B05529AE

Figure A.1 Dimensions of the Refrigerated and Nonrefrigerated Centrifuges

Installation

Electrical Requirements

A

NOTE

PN B05529AE

Additional clearance is required on the right side to allow to allow access to the power switch.

A-3

Installation

Test Run

Test Run

WARNING

To reduce the risk of electrical shock, this equipment uses a three-wire electrical
cord and plug to connect the centrifuge to earth-ground. To preserve this safety
feature:

• Make sure that the matching wall outlet receptacle is properly wired and
earth-grounded. Check that the line voltage agrees with the voltage listed on
the name rating plate affixed to the centrifuge.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of
multiple-outlet receptacle strip.

NOTE

The centrifuge must be plugged in and the power switch turned to on position (I) before the door

can be opened.

We recommend that you make a test run to ensure that the centrifuge is in proper operating
condition following shipment. See CHAPTER 2, Operation for instructions on operating the
centrifuge.

After completing the test run, log on to www.beckmancoulter.com to register your centrifuge. This
validates the centrifuge warranty and ensures your receipt of further information regarding new
accessories and/or modifications as they become available.

A-4

PN B05529AE

Beckman Coulter, Inc.

Allegra X-30 Series Centrifuge Warranty

Subject to the exceptions and upon the conditions specified below, Beckman Coulter agrees to correct, either
by repair, or, at its election, by replacement, any defects of material or workmanship which develop within
one (1) year after delivery of the Allegra X-30 series centrifuge (the product), to the original Buyer by
Beckman Coulter or by an authorized representative provided that investigation and factory inspection by
Beckman Coulter discloses that such defect developed under normal and proper use.

Some components and accessories by their nature are not intended to and will not function for as long as one
(1) year. A complete list of such components or accessories is maintained at the factory and at each
Beckman Coulter District Sales Office. The lists applicable to the products sold hereunder shall be deemed to
be part of this warranty. If any such component or accessory fails to give reasonable service for a reasonable
period of time, Beckman Coulter will repair or, at its election, replace such component or accessory. What
constitutes either reasonable service and a reasonable period of time shall be determined solely by
Beckman Coulter.

Replacement

Any product claimed to be defective must, if requested by Beckman Coulter, be returned to the factory,
transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless
the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.

Conditions

Beckman Coulter shall be released from all obligations under all warranties either expressed or implied, if the
product(s) covered hereby are repaired or modified by persons other than its own authorized service
personnel, unless such repair in the sole opinion of Beckman Coulter is minor, or unless such modification is
merely the installation of a new Beckman Coulter plug-in component for such product(s).

Disclaimer

IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS
AND OF THE WARRANTY OF MERCHANTABILITY AND THAT NEITHER BECKMAN COULTER, INC. NOR ITS
SUPPLIERS SHALL HAVE ANY LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND
WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR
REPLACEMENT OF THE PRODUCT.

PN B05529AE

Warranty-1

Beckman Coulter, Inc. Allegra X-30 Series Centrifuge Warranty

Warranty-2

PN B05529AE

www.beckman.com

Related Documents

Chemical Resistances for Beckman Coulter
Centrifugation Products

PN IN-175

Available in hard copy or electronic pdf by
request.

Available at www.beckman.com

© 2018 Beckman Coulter, Inc.

All Rights Reserved