Beckman Coulter Allegra X-14 Instructions For Use Manual

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Instructions For Use

Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.

Allegra X-14 Series

Centrifuges

Rx Only in the U.S.A.

PN B07493AD January 2018

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Allegra X-14 Series Centrifuges

Beckman Coulter Eurocenter S.A. 22, rue Juste-Olivier Case Postale 1044 CH - 1260 Nyon 1, Switzerland Tel: +41 (0) 22 365 36 11

for In-Vitro Diagnostics

PN B07493AD (January 2018)

Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

If you have any questions, contact our Customer Support Center.

• Worldwide, find us via our website at

www.beckmancoulter.com/customersupport/support.

• In the USA and Canada, call us at 1-800-369-0333.

• Outside of the USA and Canada, contact your local

Beckman Coulter Representative.

Original Instructions

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Revision History

For labeling updates, go to techdocs.beckmancoulter.com and download the latest version of the manual or system help for your instrument.

Issue AD, 01/18

Changes were made to: ECREP address.

Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin of the amended page.

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Revision History

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Safety Notice

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate the instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.

Alerts for Danger, Warning, Caution, Important, and Note

DANGER

DANGER indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

IMPORTANT

Following the advice in the Important adds benefit to the performance of a piece of equipment or to a process.

NOTE

or servicing of this equipment.

IMPORTANT is used for comments that add value to the step or procedure being performed.

NOTE is used to call attention to notable information that should be followed during installation, use,

Safety During Installation and/or Maintenance

This centrifuge weighs 128 kg (283 lb). Do not attempt to lift or move it without assistance.

Any servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury. Make sure that the power switch is turned off and the instrument is disconnected from the main power source by removing the Mains (power) plug from the outlet receptacle, and refer such servicing to qualified personnel.

Do not replace any centrifuge components with parts not specified for use on this instrument.

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Safety Notice

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the centrifuge.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet

receptacle strip.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into the centrifuge and damage electrical or mechanical components.

Safety Against Risk of Fire

This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol) in this centrifuge nor handle or store them within the 30-cm (1-ft) area surrounding the centrifuge.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the rotors and accessories designed for use in this centrifuge.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop the rotor by hand.

• Do not lift or move the centrifuge while the rotor is spinning.

• NEVER attempt to override the door interlock system while the rotor is spinning.

• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running. During

operation you should come within the envelope only to adjust instrument controls, if necessary. Never bring any flammable substances within the 30-cm (1-ft) area surrounding the centrifuge. Never lean on the centrifuge or place items on the centrifuge while it is operating.

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Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless all necessary safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to their use.

• Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi —further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health and safety guidelines.

It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter.

Safety Notice

Chemical and Biological Safety

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Safety Notice

Chemical and Biological Safety

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Contents

Revision History,iii

Safety Notice,v

Introduction,xiii

CHAPTER 1: Description,1-1

Introduction,1-1

Centrifuge Function and Safety Features,1-1

Centrifuge Function,1-1 Safety Features,1-2

Centrifuge Chassis,1-2

Housing,1-2 Door,1-3 Rotor Chamber,1-3 Temperature Sensing and Control,1-3 Drive,1-3

Controls and Indicators,1-3

Power Switch,1-3 Control Panel,1-4

Digital Displays,1-4 Run-Parameter Keys,1-5

Name Rating Plate,1-6

Specifications,1-7

Available Rotors,1-9

CHAPTER 2: Operation,2-1

Introduction,2-1

Summary of Run Procedures,2-1

Manual Run,2-1 Programmed Run,2-2

Preparation,2-3

Installing the Rotor,2-3

Manual Operation,2-4

Selecting a Rotor,2-4 Entering Run Speed,2-4 Entering Run Time,2-5 Entering Run Temperature,2-6 Entering Acceleration and Deceleration Rates,2-6 Starting a Run,2-7 Pulse Function,2-8

Programmed Operation,2-8

Page 10

Creating a New Program,2-9 Recalling a Program,2-9

Disabling/Enabling the Audible Tones,2-10

CHAPTER 3: Troubleshooting,3-1

Introduction,3-1

User Messages,3-1

Related Documents

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Illustrations

1.1 The Centrifuge,1-2

1.2 Control Panel,1-4

3.1 Door Latch Override,3-6 A.1 Centrifuge Dimensions,A-2

Illustrations

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Tables

2.1 Acceleration and Deceleration Rates (in Minutes:Seconds) to and from Maximum Speed,2-6

3.1 Diagnostic Message Chart,3-2

3.2 Troubleshooting Chart,3-5

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Intended Use

For In Vitro Diagnostic Use

This Allegra X-14 centrifuge is intended for the separation of components through the use of relative centrifugal force.

It is designed to separate human samples, including blood and other body fluids, for processing, analysis, and in vitro diagnostic testing, as well as nonhuman body samples and chemicals, including industrial and environmental samples.

This centrifuge should be operated by qualified personnel only.

Certification

Introduction

Beckman Coulter Allegra X-14 series centrifuges are manufactured in a facility that has certifications to both ISO 9001 2008 and ISO 13485 2003. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies. Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com.

Scope of Manual

This manual is designed to familiarize you with the Beckman Coulter Allegra X-14 series centrifuges, its functions, specifications, operation, and routine operator care and maintenance. We recommend that you read this entire manual, especially the Safety Notice and all safety-related information, before operating the centrifuge or performing instrument maintenance.

• CHAPTER 1, Description contains system specifications and a brief physical and functional

description of the centrifuge, including the operating controls and indicators.

• CHAPTER 2, Operation contains centrifuge operating procedures.

• CHAPTER 3, Troubleshooting lists diagnostic messages and other possible malfunctions,

together with probable causes and suggested corrective actions.

• contains procedures for routine operator care and maintenance, as well as a brief list of supplies and replacement parts.

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Introduction

Conventions

• APPENDIX A, Installation provides requirements for preparing laboratory facilities and

installing the centrifuge.

NOTE

If the centrifuge is used in a manner other than specified in this manual, the safety and performance of this equipment could be impaired. Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.

Conventions

Certain symbols are used in the product labeling to call out safety-related and other important information. These international symbols may also be displayed on the centrifuge and are reproduced on the inside of the back cover of this manual.

Typographic Conventions

Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as keys and displays.

• Key names (for example,

CFC-Free Centrifugation

START

ROTOR

) appear in capital letters in boldface type.

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Allegra X-14 series centrifuges.

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Introduction

This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-14 Series centrifuges. The operating controls and indicators are also described; instructions for their use are in CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.

Centrifuge Function and Safety Features

Centrifuge Function

CHAPTER 1

Description

The Beckman Coulter Allegra X-14R (refrigerated) or Allegra X-14 (constant controlled temperature) centrifuge (see Figure 1.1) is a benchtop centrifuge that generates centrifugal forces required for a wide variety of applications. Together with the Beckman Coulter rotors designed for use in this centrifuge, the centrifuge applications include:

• Routine processing such as sample preparations, pelleting, extractions, purifications, concentrations, phase separations, receptor binding, and column centrifugations.

• Cell isolation.

• Binding studies and separation of whole blood.

• Processing large numbers of small-volume samples in multiwell plates for concentrating tissue-

culture cells, cloning and replicate studies, in-vitro cytotoxicity studies, receptor binding, and genetic engineering experimentation.

• Rapid sedimentation of protein precipitates, large particles, and cell debris.

The Allegra X-14R and Allegra X-14 are microprocessor-controlled, providing interactive operation. The instrument design features a brushless asynchronous motor, automatic rotor identification system, program memory that enables repeated run conditions, temperature control system, and a choice of acceleration and deceleration rates. User messages alert the operator to conditions that may need attention.

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Description

Centrifuge Chassis

Figure 1.1 The Centrifuge

Safety Features

The Allegra X-14 series centrifuges have been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft). Safety features include the following.

• An electromechanical door lock system prevents operator contact with spinning rotors and prevents run initiation unless the door is shut and locked. The door is locked when a run is in progress and can be opened only when the rotor is stopped and the LED (light-emitting diode) next to the sample recovery.

• A steel barrier surrounds the rotor chamber to provide full operator protection.

• A rotor identification system prevents the installed rotor from running above its maximum

rated speed. During acceleration the microprocessor checks the magnetic rotor identification. Speed is limited to the maximum safe speed of the identified rotor. If the system identifies a rotor different than the one entered by the user, the system displays an error message and reduces the speed to the maximum allowable speed of the rotor.

• An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor loads are severely out of balance. At low speeds, an incorrectly loaded rotor can cause imbalance. Rotor instability can also occur if the centrifuge is moved while running, or if it is not resting on a level and secure surface (see CHAPTER 3, Troubleshooting).

Centrifuge Chassis

Housing

DOOR

key is lit. If there is a power failure, the door lock can be manually tripped for

The centrifuge housing is made of sheet steel and structural foam and is finished with acrylic baking enamel. The control panel overlay is made of coated polystyrene copolymer.

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Door

The aluminum and structural foam door is secured to the housing by solid hinges. A window in the center allows strobe viewing. When the door is closed, the locking system engages. The door is locked when a run is in progress and can be opened only when the rotor is stopped and the LED next to the

DOOR

key is lit. If there is a power failure, the door lock can be manually tripped for sample

recovery (see CHAPTER 3, Troubleshooting).

Rotor Chamber

The aluminum rotor chamber is coated with epoxy enamel for corrosion resistance. The chamber is sealed by a foam gasket.

Temperature Sensing and Control

With the power on, the temperature control system is activated when the door is closed and locked. A sensor in the rotor chamber continuously monitors chamber temperature. The microprocessor calculates the required chamber temperature to maintain the selected rotor temperature. Run temperature can be set between 2 and +40°C.

Description

Controls and Indicators

NOTE

To avoid chamber icing, refrigeration is off when the door is open. The centrifuge door must be closed

and locked for the refrigeration system to begin operating.

Drive

The asynchronous direct-drive motor is brushless for clean, quiet operation. The resilient suspension ensures that loads will not be disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs during centrifugation. Maximum acceleration and deceleration may be selected to allow fast processing of samples; alternately, delicate gradients may be preserved using slower acceleration and deceleration.

Controls and Indicators

Power Switch

The power switch, located on the left side of the centrifuge, controls electrical power to the centrifuge. It is also a circuit breaker that will trip to cut off power in the event of a power overload. The power switch must be turned on before the chamber door can be opened.

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Description

PULSE

ENTER/

SAVE

Controls and Indicators

Control Panel

The control panel (see Figure 1.2) is mounted at an angle on the centrifuge front for easy visibility and access. It is used to enter run parameters via function keys and a keypad, and to display run parameters, program information, and user messages.

Figure 1.2 Control Panel

Digital Displays

The displays provide run information, user messages, and diagnostics.

• During a run, they show the actual (real-time) operating conditions.

• When the run parameters are being entered, they show the set values selected. Set values can

be recalled during operation by pressing

SHOW SET

. After 5 seconds, the displays return to

actual conditions.

• Error messages (see Section 4) also appear on the displays, when applicable. The centrifuge emits a series of audible tones to alert the user to an error condition. (Instructions for disabling the tones are in CHAPTER 2.)

Indicates rotor speed in revolutions per minute (RPM) or in relative centrifugal field, a term describing the ratio of the centrifugal acceleration at a specified radius and

SPEED

TIME

speed to the standard acceleration of gravity (RCF  g).

• In a timed run, indicates the remaining run time in hours and minutes. The time display begins counting down when ENTER/SAVE then START is pressed and continues counting down to 0. The run automatically ends when the set time reaches 0 and deceleration begins (a tone sounds when the rotor stops spinning). If a malfunction shuts down the centrifuge during a run, when the instrument is turned back on the time will not remain and 00:00 will be displayed in the time window. By comparing this time with the original set time, you can determine when the run ended.

• In a hold run, indicates the time elapsed since START was pressed. After 99 hours and 59 minutes the timer resets to 0 and continues counting elapsed time.

• In a pulse (momentary) run, indicates the time (in minutes and seconds) elapsed since PULSE was pressed. Time continues to count up until the PULSE key is released. The total elapsed time is then displayed until another key is pressed.

Indicates rotor temperature in degrees C at thermoequilibrium.

TEMPERATURE

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Displays the name of the selected rotor.

ROTOR

When the ACCEL key is pressed, one of ten available acceleration rates can be entered. Displays the selected acceleration rate throughout the run.

ACCEL

When the DECEL key is pressed, one of eleven available deceleration rates can be entered. Displays the selected deceleration rate throughout the run.

DECEL

• During setup—when the PROGRAM key is pressed, a program number (one of ten

PROGRAM

• During centrifugation— the number of the program being run is displayed.

Run-Parameter Keys

possible) can be selected and program parameters entered.

Description

Controls and Indicators

Run-parameter keys are used to enter specific run information as follows. The centrifuge emits an audible tone when each key is pressed. (Instructions for disabling the tone are in CHAPTER 2).

Pressed to display scrollable list of compatible rotors. Can be pressed repeatedly to scroll through the rotor list (or the arrow keys can be used for scrolling).

If the display shows speed in RPM, the key is pressed once to enter run speed (using the keypad) in RPM (revolutions per minute); pressed twice to enter run speed in RCF (relative centrifugal field). If the display shows speed in RCF, the key is pressed once to enter speed in RCF or twice to enter speed in RPM.

Pressed to enter run time using the keypad. When TIME is pressed twice, HOLD mode is selected. (Pressing it again reverts to time-entry mode.)

• Timed run—Run time up to 99 hours and 59 minutes can be set. If more than

59 minutes are entered in the minutes field, the system automatically converts the entry to hours and minutes. Deceleration begins when the set time counts down to 0.

• Continuous run (hold)—For runs of unspecified lengths, hold mode is used. When

99 hours, 59 minutes is reached, the system resets to 0 and continues counting. A hold run continues until the STOP key is pressed.

Pressed to enter temperature (using the keypad), from 2 to +40°C. If a new temperature is not entered, the centrifuge uses the temperature set for the previous run.

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Enables selection of one of ten preset acceleration rates (see Table 2.1 in CHAPTER 2) available to protect specific gradient and sample-to-gradient interfaces. If no acceleration rate is selected, the centrifuge defaults to maximum acceleration.

Enables selection of one of eleven preset deceleration rates (see Table 2.1 in

CHAPTER 2) available to maintain optimum separation. If no deceleration rate is

selected, the centrifuge defaults to maximum deceleration. (Deceleration rate 0 is always coast to complete stop.)

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Description

Name Rating Plate

A toggle switch used to enter into and exit out of the program mode to create or recall programs. When the key is pressed, the program number can be selected using the keypad or arrow keys.

Up and down arrow keys, which can be pressed to increment parameter values up or down.

The ENTER/SAVE key can be pressed to save parameter inputs to system memory during manual run setup or to a program during a program set up, and to start the centrifuge (ENTER/SAVE, then START must be pressed to start the centrifuge).

The keypad is used to enter numerical run parameters, to enter or recall a program number, and to select acceleration and/or deceleration rates. In addition to keys 0 through 9, the keypad includes a plus/minus key and a CE (clear entry) key.

The CE (clear entry) key clears any parameter on the display. Pressing CE will also clear some diagnostic messages. If a diagnostic message does not clear when CE is pressed, see CHAPTER 3, Troubleshooting.

Name Rating Plate

Pressed during a run to display set values for 5 seconds.

Causes the rotor to accelerate, at maximum rate, to set speed for short-duration runs (as long as the key is pressed). Deceleration, at maximum rate, begins when the key is released.

Pressing ENTER/SAVE, then START begins the run. (START must be pressed within 5 seconds of pressing ENTER/SAVE.) This key combination can also be used to abort a deceleration process and restart the centrifuge.

Can be pressed at any time while the rotor is spinning to terminate a run. The centrifuge decelerates to a complete stop according to the deceleration setting. The green light next to the key flashes while the rotor decelerates. A tone sounds when the rotor comes to a complete stop. (Instructions for disabling the tone are in

CHAPTER 2.)

Unlocks and opens the door. (The instrument will not accept the unlock command if the rotor is spinning.)

A name rating plate is affixed to the rear of the instrument. Always mention the serial number and model number when contacting Beckman Coulter regarding your centrifuge.

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Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee.

Specification Description

Speed • Set speed

Time • Set time

Temperature • Set Temperature

Humidity restrictions <75% (noncondensing)

Description

Specifications

— 200 to 10,200 RPM in 10-RPM increments

• Speed display — actual rotor speed in 10-RPM increments or in RCF (when

selected)

— to 99 hours 59 minutes or continuous (hold)

• Time display — Timed run: indicates run time remaining — Continuous (hold) or pulse run: indicates elapsed time

— Allegra X-14R is 2 to +40°C in 1° increments — Allegra X-14 is factory set at 20°C

• Temperature control (after equilibration)

— Allegra X-14R is 2°C of set temperature — Allegra X-14 is 2°C of the 20°C set temperature

• Temperature display (after equilibration) — chamber temperature in 1° increments

• Ambient temperature range — 10 to 30°C

Acceleration 10 acceleration rates

Deceleration 11 deceleration rates

Dimensions • Width

Weight 128 kg (283 lb)

Ventilation clearances (sides and rear)

Finishes • Control panel

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— 76.2 cm (30.0 in.)

• Depth — 62.2 cm (24.5 in.)

• Height — 34.3 cm (13.5 in.)

7.6 cm (3.0 in.)

— polycarbonate

• Housing surfaces — acrylic baking enamel

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Description

Specifications

Specification Description

Electrical requirements • 120-V, 60-Hz instrument

— 108–132 VAC, 12 A, 60 Hz

Electrical supply Class I

Maximum heat dissipation into room

4318 Btu/hr (1.3 kW)

under steady-state conditions

Noise level 0.91 m (3 ft) in front of

 64 dBa

centrifuge

Installation (overvoltage) category II

Pollution degree

a. During transient conditions, such as acceleration and deceleration, rotor temperature may be outside this range. To reach

temperatures above ambient, the centrifuge is dependent on the frictional heat generated inside the chamber during operation. At low run speeds or low ambient temperatures, the centrifuge may not be able to achieve some higher temperatures.

b. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation

must be expected.

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Available Rotors

The following Beckman Coulter rotors can be used in the Allegra X-14 series centrifuges. The rotors are described in individual manuals that accompany each rotor.

Rotor Profile Description

Rotor

Selection

Code

Max

RPM

Max RCF

( g)

at r

max

Number

of Tubes 

Nominal

Capacity

Description

Available Rotors

Rotor

Manual

Number

FX6100 Fixed Angle

= 98.0 mm

max

SX4750 Swinging Bucket

Tube-and-bottle buckets,

= 207.8 mm

max

Multiwell-plate carriers,

= 183.2 mm

max

SX4750A Swinging Bucket (ARIES)

Tube-and-bottle buckets,

= 207.8 mm

max

Multiwell-plate carriers,

= 183.2 mm

max

FX6100 10,200 11,419 6  100 mL GX-TB-005

GX-TB-003

SX4750

SX4750

4,300

4,000

4,303

3,283

4  750 mL

4  96 mL

GX-TB-004

SX4750A

SX4750a

4,300

4,000

4,303

3,283

4  750 mL

4  96 mL

a. Maximum speeds are based on a solution density of 1.2 g/mL. At upper temperature and humidity ambient conditions, swinging bucket rotor

speed may require reduction.

b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (r

gravity (g) according to the following formula: RCF = r (2  RPM/60), and g is the standard acceleration of gravity (9807 mm/s

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/g — where r is the radius in millimeters,  is the angular velocity in radians per second

). After substitution: RCF = 1.12 r (RPM/1000)2.

) to the standard acceleration of

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Description

Available Rotors

1-10

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Introduction

This section contains centrifuge operating procedures. A summary is provided at the start of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation.

CHAPTER 2

Operation

WARNING

Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

Do not use the centrifuge in the vicinity of flammable liquids or vapors, and do not run such materials in the instrument. Do not lean on the centrifuge or place items on it while it is operating.

Summary of Run Procedures

For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration.

Manual Run

Turn the power switch on (I).

Press

DOOR

to open the chamber door; lift the door open.

Install the rotor according to the applicable rotor manual, then close the chamber door.

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Operation

Summary of Run Procedures

Press

ROTOR

Press

RPM/RCF

toggle between RPM and RCF modes.

Press

TIME

twice to toggle between hold (continuous) run and set time mode.

Press

TEMP °C

Press

ACCEL

1 (SLOW

, then press

, then use the keypad to enter the run speed. Press

, then use the keypad to enter the run time (to 99 hrs, 59 minutes); or press

, then use the keypad to enter the required run temperature 2 to +40°C.

, then use the keypad or arrow keys to enter the selected acceleration rate number,

) through 10 (

ROTOR

MAX

again or use the arrow keys to select the rotor.

RPM/RCF

a second time to

TIME

Press

0 (OFF

Check that all parameters are correct and that the door is closed (door LED will be lit). Press

ENTER/SAVE,

Wait for the set time to count down to zero, or end the run by pressing

When the rotor stops (a tone sounds and the door; lift the door.

Programmed Run

Turn the power switch on (I).

Press

DECEL

, then use the keypad or arrow keys to enter the selected deceleration rate number,

) through 10 (

then press

DOOR

to open the chamber door.

MAX

START

(within 5 seconds).

DOOR

LED lights), press

STOP

DOOR

to open the chamber

Install the rotor according to the applicable rotor manual, then close the chamber door.

Press

PROGRAM

press

ENTER/SAVE

2-2

, use the keypad or arrow keys to enter the required program number, then

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Preparation

Prepare the rotor for centrifugation as described in the applicable rotor manual. For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration.

Operation

Preparation

Check that all parameters are correct and that the door is closed (door LED will be lit). Press

ENTER/SAVE

Wait for the run to end, or end the run by pressing

When the rotor stops (a tone sounds and the door; lift the door.

, then press

START

(within 5 seconds).

STOP

DOOR

LED lights), press

DOOR

to open the chamber

Installing the Rotor

The power must be turned on before you can unlock and open the chamber door. (To end a run for any reason, do not turn the power switch off; press

Turn the power switch on.

• The control panel display shows “

Press

DOOR

LED is lit.

• The door opens and the LED goes out.

Install the rotor according to directions in the rotor manual.

a. Ensure that the rotor is seated on the drive hub. b. Avoid bumping the control panel keys during rotor installation or removal.

Close the chamber door by pressing down firmly on both sides.

• The door locks.

STOP

instead.)

Allegra X-14

. The centrifuge will accept this command only when the rotor is at rest and the

” or “

Allegra X-14R

” and the software version.

• When the latches engage, the LED next to the

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DOOR

key lights up.

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Operation

Manual Operation

NOTE

When using multiwell-plate carriers, enter the appropriate rotor, either SX4750 or SX4750a. The speed will be automatically limited to 4000 RPM. (The 4000 RPM limit is valid when running up to three stacked Beckman Coulter plates separated by cap strips. Reduce speed to 2700 RPM if running four stacked plates).

Set rotor speed using RPM values only. To set speed to a specific RCF, use the RPM-to-RCF conversion chart (GX-TB-015) to determine the applicable RPM to enter.

When a run-parameter key ( parameter can be entered or changed.

• Entry is completed when

To change an entry before you’ve pressed different value.

a. Press b. To change an entry after you’ve pressed

If an unacceptable value is entered, an out of range message is displayed in the message line.

a. Enter the correct value.

to abort changes and start over.

ROTOR, RPM/RCF, TIME, TEMP, ACCEL

ENTER/SAVE

or another function key is pressed.

ENTER/SAVE

or another parameter key, enter a

, press the run-parameter key again.

, or

DECEL

) is pressed, a

Selecting a Rotor

Press

ROTOR

Press the next run-parameter key or press

Entering Run Speed

Enter a run speed up to the maximum speed of the rotor in use. Or, enter a relative centrifugal field (RCF) value up to the maximum achievable RCF of the rotor.

Press

RPM/RCF

modes.

• Allowed speed range for the selected rotor is displayed.

, then use the arrow keys or press

ENTER/SAVE

on ce t o en ter spee d in RPM . Pr ess

ROTOR

RPM/RCF

again to select a rotor.

again to toggle between

RPM

and

RCF

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Page 29

Use the keypad to enter required speed.

• Entered speed appears on the speed display.

• (If the entered speed is out of the speed range of the installed rotor, an error message will

appear and the input will be cleared.

a. Enter an acceptable value from the range displayed.)

Press the next run-parameter key or

• The rotor will accelerate or decelerate to the new speed.

• You can change the set speed at any time during a run by repeating steps 1 through 3.

Entering Run Time

ENTER/SAVE

Operation

Manual Operation

Run time can be set for up to 99 hours and 59 minutes or for a hold (continuous) run. The time display begins counting down when the rotor starts to spin. For a timed run, the run automatically terminates when the set time reaches zero and deceleration begins at the selected rate; a tone sounds when the rotor has stopped. For a hold run, the elapsed time is displayed and the run continues until

Press

• If

Use the keypad or arrow keys to enter required time.

• Entered time appears on the display.

• If you entered a number higher than 59 minutes, the centrifuge automatically recalculates

the time in hours and minutes. or

a. Press

•

Press the next run-parameter key or

STOP

is pressed.

TIME

HOLD

is displayed, press

TIME

again for a hold run.

HOLD

appears on the display.

TIME

again to enter time.

ENTER/SAVE

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Page 30

Operation

Manual Operation

Entering Run Temperature

Run temperature can be set from 2 to +40°C. If no value is entered, the centrifuge selects the last entered temperature.

Press

TEMP

• The cursor and the temperature range (2 to 40°C) appear.

Use the keypad or arrow keys to enter required temperature.

• Entered temperature appears on the display.

• (If the entered temperature is outside the valid temperature range, an error message

will appear and the input will be cleared.

a. Enter a valid temperature.)

Press the next run-parameter key or

ENTER/SAVE

Entering Acceleration and Deceleration Rates

You can select one of ten acceleration rates and eleven deceleration rates to protect the gradient and sample-to-gradient interface. Select rates depending on the type of run you are performing—

for pelleting runs, where sample mixing is not a concern, maximum acceleration and deceleration can be used. If running delicate gradients, a lower setting may be needed. Typical acceleration and deceleration rates are listed in Table 2.1. If no rate is selected, the centrifuge automatically accelerates and decelerates at maximum.

Table 2.1 Acceleration and Deceleration Rates (in Minutes:Seconds) to and from Maximum Speed

Rate SX4750 SX4750 SX4750A SX4750a FX6100

(4300 RPM) (4000 RPM) (4300 RPM) 4000 RPM (10,200 RPM)

Accel Decel Accel Decel Accel Decel Accel Decel Accel Decel

10 (MAX)

1:24

2:03

2:14

2:27

1:21

2:07

2:18

2:33

1:14

1:53

2:04

2:16

1:14

1:59

2:08

2:21

1:23

2:00

2:14

2:27

1:21

2:06

2:19

2:30

1:14

1:50

2:04

2:17

1:16

1:58

2:09

2:23

1:24

2:23

3:24

4:28

1:23

2:26

3:25

4:23

2-6

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Operation

Manual Operation

Table 2.1 Acceleration and Deceleration Rates (in Minutes:Seconds) to and from Maximum Speed

Rate SX4750 SX4750 SX4750A SX4750a FX6100

(4300 RPM) (4000 RPM) (4300 RPM) 4000 RPM (10,200 RPM)

Accel Decel Accel Decel Accel Decel Accel Decel Accel Decel

2:33

2:53

3:20

4:00

4:58

6:36

—

, then use the keypad or arrow keys to select the required acceleration rate.

Press

2:49

3:15

3:42

4:26

5:29

7:16

19:41

ACCEL

1 (SLOW)

0 (OFF)

a. Times shown are with the rotor fully loaded in the Allegra X-14R. (Times are approximate; actual times will vary depending on the rotor in use,

the rotor load, run speed, and voltage fluctuations.)

2:45

3:07

3:36

4:18

5:21

7:06

—

2:38

3:00

3:27

4:08

5:06

6:46

16:12

2:45

3:07

3:36

4:19

5:20

7:06

—

2:51

3:12

3:44

4:26

5:29

7:19

19:27

2:33

2:54

3:20

4:01

4:59

6:36

—

2:38

3:02

3:28

4:09

5:07

6:46

15:53

5:26

6:27

7:31

8:33

9:36

10:11

—

• Entered acceleration rate appears on the display.

5:29

6:30

7:29

8:33

9:31

10:07

18:32

Press the next run-parameter key or

Press

• Entered deceleration rate appears on the display.

Press the next run-parameter key or

Starting a Run

NOTE

Press

• The light next to the

ENTER/SAVE

DECEL

, then use the keypad or arrow keys to select the required deceleration rate.

ENTER/SAVE

Wipe out moisture from the chamber and the chamber gasket before each run.

ENTER/SAVE

NOTE

you wait more than 5 seconds, the START key will not activate. If this happens, press ENTER/SAVE and START again to begin the run.

and

START.

START

key flashes and the rotor begins to spin.

To begin a run, ENTER/SAVE must always be the last key pressed before pressing START. If

• The run will end when the time display counts down to zero.

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Page 32

Operation

Programmed Operation

a. (Press

STOP

to terminate a run for any reason.)

• The green light next to the

STOP

key will flash while the rotor decelerates.

• A tone sounds when the rotor comes to a complete stop. — (Instructions for disabling the tone are at the end of this section.)

After the rotor has stopped and the LED by the door key lights, press

DOOR

• The door opens.

Remove the rotor (or containers).

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

NOTE

Depending upon the duration of the event, transient power-line interruptions may cause this equipment to decelerate or reset with the possible loss of in-process operation. If you suspect this has occurred, simply repeat the runs.

Pulse Function

The pulse function, accessed by pressing the

PULSE

• When the

key is pressed, the rotor accelerates at maximum rate to the set speed and continues to spin as long as the deceleration settings are overridden by the pulse function.) When the the rotor begins decelerating to 0 RPM using maximum deceleration. Using the pulse function eliminates the need to press the

• When the the

PULSE

key is pressed, the

PULSE

key is released, the seconds stop accumulating.

• The centrifuge memory retains the parameters of the last run performed before the was pressed. At the end of a pulse run, after the centrifuge door is opened and closed the previous run parameters are displayed.

Programmed Operation

The instrument internal memory can store up to 10 programs which can be recalled by keypad numbers 0 through 9. Saved programs are retained in memory even if the power is turned off. Procedures for entering run parameters are the same for programmed operation as those described above for manual operation.

PULSE

key, is used for short-duration runs as follows.

PULSE

key is pressed. (The current run time, acceleration, and

PULSE

key is released,

START

and

STOP

keys.

TIME

display begins displaying the elapsed seconds. When

PULSE

key

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Page 33

Creating a New Program

Press

PROGRAM

use, or one that you want to overwrite, and press

• The program number flashes.

Enter run parameters (rotor, speed, time, temperature, acceleration/deceleration rate settings). Press

• If you entered an unacceptable parameter, an error message will be displayed.

Operation

Programmed Operation

, then use the keypad or arrow keys to select a program number that is not in

ENTER/SAVE

a. (Press

When all parameters are entered, press

• The program is saved into memory and “

• The program number displayed will stop flashing.

• If any parameters are then modified, the program number will show a dash.

Press

ENTER/SAVE

NOTE

If the PROGRAM key is pressed while the program number is flashing, program mode is exited

without saving entered parameters into program memory.

Recalling a Program

Press

PROGRAM

a. Press

and enter an acceptable value, then press the next run-parameter key.)

ENTER/SAVE

and then

, then use the keypad or arrow keys to enter the number of the program.

ENTER/SAVE

START

to run the programmed run.

to recall a program or press

Program saved

PROGRAM

” appears on the display.

to exit program mode.

• The program parameters are displayed if

Press

ENTER/SAVE

• The run starts, the green light next to the

NOTE

• The run will end automatically when the time display counts down to zero.

a. (Press

PN B07493AD

To begin a run, ENTER/SAVE must always be the last key pressed before pressing START. If you

wait more than 5 seconds, the START key will not activate. If this happens, press ENTER/SAVE and

START again to begin the run.

STOP

• The green light next to the

• A tone will sound when the rotor has come to a complete stop.

ENTER/SAVE

and

START

to begin the programmed run.

START

to terminate a run at any time.)

STOP

key will flash while the rotor decelerates.

was pressed.

key flashes, and the rotor begins to spin.

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Page 34

Operation

Disabling/Enabling the Audible Tones

After the rotor has stopped and the LED by the door key lights, press

• The door opens.

Remove the rotor (or containers).

Disabling/Enabling the Audible Tones

The audible tones (beeps) that sound when a run-parameter key is pressed, or at the end of each run, may be turned on and off by following the steps below (rotor must be at 0 RPM). The audible tone that sounds when an error occurs cannot be disabled.

To disable the key press display.

ENTER

, then press

2 3 6

. The words “

DOOR

Disable KEY beeper

” appear on the

To disable the end-of-run tone, press appear on the display.

Tones that have been disabled can be enabled as follows.

To enable a key press display.

To enable the end-of-run tone press appear on the display.

ENTER

, then press

ENTER

2 3 5

ENTER

, then press

. The words “

, then press

2 2 3

. The words “

Enable KEY beeper

2 2 2

. The words “

Disable RUN beeper

” appear on the

Enable RUN beeper

”

2-10

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Introduction

This section lists possible malfunctions, together with probable causes and corrective actions. Maintenance procedures are given in CHAPTER 4. For any problems not covered here, contact Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com) for assistance.

CHAPTER 3

Troubleshooting

NOTE

It is your responsibility to decontaminate the instrument, as well as any rotors and/or accessories,

before requesting service by Beckman Coulter Field Service.

User Messages

User messages appear on the display to communicate information about the instrument or to alert you to abnormal conditions that need attention.

• Help and informational messages caused by incorrect input or certain operating conditions can be cleared by pressing

• Diagnostic messages that result from abnormal operating conditions or equipment malfunction require troubleshooting. Refer to Table 3.1 to determine the nature of the condition and any recommended actions. If a problem persists after you have performed the recommended action, call Beckman Coulter Field Service (in the United States, call 1-800-742-2345; outside the U.S., call your local Beckman Coulter office or visit us at www.beckmancoulter.com). To help the field service representative diagnose and correct the problem, gather as much information about the situation as you can, including:

— the diagnostic number and message, — the operating situation when the diagnostic condition occurred (such as rotor in use,

— any unusual environmental and/or operating conditions (such as ambient temperature or

and following the instructions in the message.

speed, or load type), and

voltage fluctuations).

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Page 36

Troubleshooting

User Messages

Table 3.1 Diagnostic Message Chart

Diagnostic

Number/Message

Problem Result Recommended Action

P1–

Power failure occurred, see manual

L1 through L6– Door latch, call Service

L7– Can’t open door, see manual

C1– Rotor temp exceeds 4C above set

C2– Rotor temp exceeds 8C above set, call Service

System or facility power failure

Latches are not operating properly

Door latches do not release

Rotor temperature exceeds temperature setting by more than 4°C but less than 8°C after equilibration

Rotor temperature exceeds temperature setting by more than 8°C after equilibration

Run shuts down Press CE to clear message. Restart run.

Remaining time shows on display.

Error message appears; run shuts down with no brake

Door doesn’t open when DOOR key is pressed

Press down on both sides of the door until the DOOR LED comes on. If you close the door repeatedly and the problem continues, call

Beckman Coulter Field Service.

Press down on the door, then press DOOR again. If door still doesn’t open call Beckman Coulter Field Service. (Procedures for retrieving your sample are at the end of this section.) If the door opens but the message stays on the display, press CE to clear.

Run continues; the

Press CE to clear message.

message is informational

Run shuts down Call Beckman Coulter Field Service.

C3 and C5– Temp error, call Service

T1 and T2– Temp, door locks for 45 minutes, call Service

T3– Temp, door locks for 45 minutes, see manual

D1 through D9– Drive error, call Service

Refrigeration system error

Run shuts down with maximum brake

System temperature problem

Run shuts down with no brake; door locks for 45 minutes

Motor overheated Run shuts down

with no brake; door locks for 45 minutes

Drive system problem Run shuts down

with no brake

Call Beckman Coulter Field Service.

Wait 45 minutes for motor to cool down, then restart run. If problem persists, call Beckman Coulter Field Service.

Call Beckman Coulter Field Service. Before trying to open the door, listen carefully and make sure that no sound is coming from the chamber (indicating a spinning rotor). Follow the directions under Retrieving Your Sample in

Case of Power Failure, below.

3-2

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Page 37

Troubleshooting

User Messages

Table 3.1

Number/Message

R1 and R2–

Rotor ID problem, call Service

R3– Rotor ID mismatch, check rotor

R4 and R5– No rotor match, see manual

S1– System error, call Service

Diagnostic Message Chart (Continued)

Diagnostic

Problem Result Recommended Action

No magnets identified, or magnets incorrectly identified

The entered rotor number is not the same as the rotor identified

The system cannot identify the rotor

There is a problem with the system control software, EPROM, or RAM

Run shuts down with maximum brake

System shuts down, no brake

• Make sure the rotor in use is a compatible

Beckman Coulter rotor (see Available

Rotors in CHAPTER 1). Check that magnets

are in place on rotor bottom. Press CE to clear the message.

• Call Beckman Coulter Field Service.

Press CE to clear message. Enter the correct rotor entry identification.

• Make sure the rotor in use is a compatible

Beckman Coulter rotor (see Available

Rotors in CHAPTER 1). Check that magnets

are in place on rotor bottom. Press CE to clear the message.

• Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

S8– System error, door locks for 45 minutes, see manual

H1, H2, and H4– Speed error, call Service

H3, H5, and H6– Speed error, door locks for 45 minutes, call Service

There is a problem with the system control software

Speed control problem

Run shuts down with no brake, door locks for 45 minutes

Run shuts down with maximum brake.

Run shuts down with no brake; door locks for 45 minutes

Wait 45 minutes then press CE to clear message, restart run. If problem persists, call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

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Troubleshooting

Other Possible Problems

Operating problems that may not be indicated by diagnostic messages are described in Table 3.2, along with probable causes, listed in the probable order of occurrence, and corrective actions. Perform the recommended corrective action in sequence, as listed. If you are unable to correct the problem, call Beckman Coulter Field Service.

3-4

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Page 39

Retrieving Your Sample in Case of Power Failure

Table 3.2 Troubleshooting Chart

Problem Problem/Result Recommended Action

Troubleshooting

Rotor cannot achieve set speed

Door will not open Rotor spinning Wait until the rotor stops.

Displays are blank Power not on Plug in the power cord; turn power on.

Chamber does not reach selected temperature

Line voltage below rating Have a qualified service person measure line

voltage while the instrument is operating.

Electrical failure Make sure the power cord is securely connected;

call Beckman Coulter Field Service.

Motor failure Call Beckman Coulter Field Service.

Power not on Plug in the power cord; turn power on.

Source power failure See Retrieving Your Sample in Case of Power

Failure, below.

Electrical failure Make sure the power cord is securely connected;

call Beckman Coulter Field Service.

Centrifuge cannot maintain selected temperature for rotor in use at speed selected

• Precool or prewarm rotors before running at low

or high temperatures.

• Precool rotor chamber by running a 30-minute

cycle at the required temperature with the speed set at 2000 RPM.

• Make sure the air intake vent is clear.

• If using multiwell-plates for runs exceeding

2 hours in a warm and/or humid environment, speed reduction may be required to maintain low sample temperature.

Chamber icing Excess humidity in

Retrieving Your Sample in Case of Power Failure

If facility power fails you will have to restart the run when the power is restored. In the event of an extended power failure, it may be necessary to trip the door-locking mechanism manually to remove the rotor and retrieve your sample.

Turn the power off and disconnect the power cord from the main power source.

• No displays or LEDs are lit.

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chamber

Condensation build-up between runs

Wipe out moisture from the chamber and the chamber gasket before each run.

• Leave door open between runs.

• Set temperature to a setting higher than

ambient temperature.

• Turn centrifuge power off.

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Page 40

Troubleshooting

Retrieving Your Sample in Case of Power Failure

Make sure that the rotor is not spinning.

• No sound or vibration comes from the centrifuge.

WARNING

Never attempt to override the door interlock system while the rotor is spinning.

Locate the two joined release cords underneath the bottom center of the centrifuge (not visible from the centrifuge front). See Figure 3.1.

Pull on the cords or insert a screwdriver through the two cords and pull to release.

• The latch releases and the door opens.

a. If the rotor is still spinning, close the door and wait until it stops before attempting to remove it.

WARNING

Never attempt to slow or stop the rotor by hand.

Figure 3.1 Door Latch Override

1. Release cord

3-6

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Page 41

Introduction

This section contains care and maintenance procedures that should be performed regularly. For maintenance not covered in this manual, contact Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com) for assistance. User messages and recommended actions are discussed in

CHAPTER 3, Troubleshooting.

CHAPTER 4

Care and Maintenance

NOTE

It is your responsibility to decontaminate the centrifuge, as well as any rotors and/or accessories,

before requesting service by Beckman Coulter Field Service.

Instrument Care

WARNING

Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and/or mechanical injury. Therefore, turn the power off and disconnect the instrument from the main power source by removing the Mains (power) plug from the outlet receptacle, and refer such maintenance to service personnel.

Maintenance

Perform the following procedures regularly to ensure continued performance and long service life of the centrifuge.

Inspect the centrifuge chamber for accumulations of sample, dust, or glass particles from broken sample tubes.

a. Clean as required (see Cleaning, below).

Check the air intake and exhaust for obstructions. Keep vents clear and clean.

Wipe condensation out of the rotor chamber between runs with a sponge or clean cloth to prevent chamber icing.

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Page 42

Care and Maintenance

Instrument Care

If chamber icing occurs, defrost the system and wipe moisture out of the chamber before use.

a. To defrost the system, set the temperature to 30°C for 20 minutes.

(These are suggested settings which may be adjusted as appropriate for your laboratory conditions.)

Cleaning

NOTE

Clean the centrifuge frequently. Always clean up spills when they occur to prevent corrosives or contaminants from drying on component surfaces.

Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment.

To prevent accumulations of sample, dust, and/or glass particles from broken sample tubes, keep the chamber clean and dry by frequent wiping with a cloth or paper towel.

a. For thorough cleaning, wash the chamber using a mild detergent such as

Beckman Solution 555 (339555), diluted 10 to 1 with water.

b. Rinse thoroughly and dry completely.

Clean the centrifuge exterior surfaces by wiping with a cloth dampened with Solution 555, diluted 10 to 1 with water.

a. Do not use acetone.

Remove the rotor from the centrifuge and clean the drive hub regularly using Solution 555 (diluted 10 to 1 with water) and a soft brush.

a. Rinse thoroughly and dry completely.

Tube Breakage

If a glass tube breaks, and all the glass is not contained in the bucket or rotor, be sure to thoroughly clean the chamber.

4-2

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Page 43

WARNING

Be careful when examining or cleaning the chamber and chamber gasket, as sharp glass fragments may be embedded in their surfaces.

Examine the chamber gasket to make sure that no glass particles are retained in it.

a. Carefully remove any glass particles that may remain.

Carefully wipe away any glass particles that remain in the chamber.

Decontamination

If the centrifuge and/or accessories are contaminated with radioactive or pathogenic solutions, perform appropriate decontamination procedures. Refer to Chemical Resistances (publication IN-175) to be sure the decontamination method will not damage any part of the centrifuge.

Care and Maintenance

Circuit Breaker and Fuses

Sterilization and Disinfection

The centrifuge is finished with acrylic baking enamel. Ethanol (70%)* may be used on this surface. See Chemical Resistances for chemical compatibilities of centrifuge and accessory materials.

While Beckman Coulter has tested ethanol (70%) and found that it does not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

Circuit Breaker and Fuses

There are no user-replaceable fuses in the centrifuge.

If the centrifuge circuit breaker trips for any reason, the power switch will move to the off (O) position. Reset the circuit breaker by turning the power switch back to the on ( again immediately, do not reset it. Call Beckman Coulter Field Service.

CAUTION

Repeated attempts to reset the centrifuge circuit breaker can cause substantial damage to electrical and electronic components.

) position. If it trips

* Flammability hazard. Do not use in or near operating centrifuges.

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Page 44

Care and Maintenance

Storage and Transport

Storage

To ensure that the centrifuge does not get damaged, contact Beckman Coulter Field Service for specific instructions and/or assistance in preparing the equipment for transport or long-term storage. Temperature and humidity conditions for storage should meet the environmental requirements described under Specifications in CHAPTER 1.

Supply List

Contact Beckman Coulter Sales or visit www.beckman.com for information about ordering parts, supplies, and publications. For your convenience, a partial list is given below.

Refer to the applicable rotor manual for materials and supplies needed for rotors.

Replacement Parts

Descrilption Part Numbr

Anti-rotation kit 392988

Supplies

NOTE

For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.

Description Part Number

Silicone vacuum grease (1 oz) 335148

Beckman Solution 555 (1 qt) 339555

Program library record 233679

4-4

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Page 45

Introduction

Preinstallation requirements have been sent prior to shipment of your centrifuge.

WARNING

This centrifuge weighs 128 kg (283 lb). Do not attempt to lift or move it without assistance.

Space and Location Requirements

WARNING

APPENDIX A

Installation

Do not place the centrifuge near areas containing flammable reagents or combustible fluids. Vapors from these materials could enter the centrifuge air system and be ignited by the motor.

The centrifuge ships in a cardboard box on a wooden pallet. For easy access, remove the top of the box, the foam insert on top of the centrifuge, and then the upper part (sides) of the box and set them aside for possible future transportation/storage. Then, with the help of additional person(s) and/or equipment, move the centrifuge from the pallet to its final position.

Select a location away from heat-producing laboratory equipment, with sufficient ventilation to allow for heat dissipation.

• The centrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during operation.

Position the centrifuge on a level surface, such as a sturdy table or laboratory bench that is able to support the weight of the centrifuge (128 kg/283 lb) and resist vibration.

a. Make sure that the centrifuge front feet are fully supported on the table.

In addition to the space for the centrifuge itself, allow 7.6-cm (3-in.) clearances at the sides and back of the centrifuge to ensure sufficient air circulation.

• Dimensions are shown in Figure A.1.

• Additional clearance is required on the left side to allow access to the power switch.

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A-1

Page 46

Installation

96.5

38.0

76.2

30.0

7.6

3.0

7.6

3.0

34.3

13.5

62.2

24.5

Electrical Requirements

Ambient temperatures during operation should not be lower than 10°C (50°F) or higher than 30°C (86°F).

• Relative humidity should not exceed 75% (noncondensing).

Figure A.1 Centrifuge Dimensions

Electrical Requirements

A-2

120-V, 60-Hz centrifuge 108–132 VAC, 12 A, 60 Hz

To reduce the risk of electrical shock, this centrifuge comes with a 2.5-m (8-ft) three-wire electrical cord (attached to the power connector at the rear of the instrument) and plug to connect the centrifuge to earth-ground. The plug for use with 120-VAC, 60-Hz centrifuges is the NEMA 5-15P; a NEMA 5-15R socket should be available.

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Page 47

Installation

Securing the Centrifuge to the Bench

Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.

a. Check that the line voltage agrees with the voltage listed on the name rating plate affixed

to the centrifuge.

b. The Mains (Power) Plug is the Disconnect Device and must remain easily accessible. c. Position the centrifuge so that it is easy to remove the Mains (Power) Plug from the outlet

receptacle.

Never use a three-to-two wire plug adapter.

Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet receptacle strip.

If there is any question about voltage, have a qualified service person measure it under load while the drive is operating.

To ensure safety, the centrifuge should be wired to a remote emergency switch (preferably outside the room where the centrifuge is housed, or adjacent to the exit from that room) in order to disconnect the centrifuge from the main power source in case of a malfunction.

Securing the Centrifuge to the Bench

Allegra X-14 series centrifuges are certified to meet the centrifuge safety standards. To meet these requirements, the centrifuge must be secured to the bench using the anti-rotation kit shipped with the instrument. This will prevent the centrifuge from moving in the unlikely event of a rotor mishap.

Complete instructions for installing the anti-rotation kit are packaged with the hardware, which is shipped with the centrifuge. The instructions (publication GXR-TB-002) include a full-size template to be used as a guide for drilling holes in the bench. Refer to this document for installation instructions.

The Allegra X-14 or Allegra X-14R centrifuge may also be secured to the benchtop surface by means of a noninvasive centrifuge restraint system (NICRS), which uses an adhesive to secure the centrifuge to the benchtop. The NICRS installation kit (A79118) contains two adhesive restraining plates, mounting hardware, and easy to follow instructions to secure the Allegra X-14 or Allegra X-14R centrifuge. Contact your Beckman Coulter representative for purchasing details.

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Installation

Test Run

NOTE

The centrifuge must be plugged in and the power switch turned to on position (I) before the door

can be opened.

We recommend that you make a test run to ensure that the centrifuge is in proper operating condition following shipment. See CHAPTER 2 for instructions on operating the centrifuge.

A-4

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Beckman Coulter, Inc.

Allegra X-14 Series Centrifuge Warranty

Subject to the exceptions and upon the conditions specified below and the warranty clause of the Beckman Coulter, Inc. terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election, by replacement, any defects of material or workmanship which develop within one (1) year after delivery of an Allegra X-14 series centrifuge (the product), to the original buyer by Beckman Coulter or by an authorized representative, provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use.

Some components and accessories by their nature are not intended to and will not function for as long as one (1) year. A complete list of such components or accessories is maintained at the factory and at each Beckman Coulter District Sales Office. The lists applicable to the products sold hereunder shall be deemed to be part of this warranty. If any such component or accessory fails to give reasonable service for a reasonable period of time, Beckman Coulter will repair or, at its election, replace such component or accessory. What constitutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter.

Replacement

Any product claimed to be defective must, if requested by Beckman Coulter, be returned to the factory, transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.

Conditions

Beckman Coulter makes no warranty concerning products or accessories not manufactured by it. In the event of failure of any such product or accessory, Beckman Coulter will give reasonable assistance to the Buyer in obtaining from the respective manufacturer whatever adjustment is reasonable in light of the manufacturer’s own warranty.

Beckman Coulter shall be released from all obligations under all warranties, either expressed or implied, if the product(s) covered hereby are repaired or modified by persons other than its own authorized service personnel, unless such repair in the sole opinion of Beckman Coulter is minor, or unless such modification is merely the installation of a new Beckman Coulter plug-in component for such product(s).

Disclaimer

IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER, INC., SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.

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Beckman Coulter, Inc. Allegra X-14 Series Centrifuge Warranty

Warranty-2

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English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어

Symbol Symbol Simbolo Symbole Simbolo

Simbole

символ

符号

記号

상징

Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목

Dangerous voltage Gefährliche electrische Spannung Voltaje peligroso Courant haute tension Pericolo: alta tensione

Caution, consult accompanying documents Vorsicht, konsultieren Begleitdokumente Atención, consulta documentos adjuntos Attention, consultent des documents

d'accompagnement

Attenzione, consulta i documenti di

accompagnamento

Biohazard Potentiell infektiösem Material Riesgo biológico Risque biologiqu Pericolo biologico

On (power) Ein (Netzverbindung) Encendido Marche (mise sous tension) Acceso (sotto tensione)

Off (power) Aus (Netzverbindung) Apagado Arrêt (mise sous tension) Spento (fuori tensione)

Protective earth (ground) Schutzleiteranschluß Puesta a tierra de protección Liaison à la terre Collegamento di protezione a terra

Earth (ground) Erde (Masse) La tierra (suelo) Terre (sol) Scarica a terra

In vitro diagnostic medical device In-vitro-diagnosemedizinisches Gerät Aparato médico de diagnóstico in vitro Appareil médical diagnostique in vitro Apparecchio medico diagnostico in vitro

Tensão perigosa

Опасное напряжение тока

危险电压危険な電圧

위험한 전압

Cuidado, ulta documentos adjuntos

Внимание, советует с сопроводительными

документами

注意, 咨询附属单证注意, 伴う文書に相談しなさい 주의, 동반 문서를 상담하십시오

Material infeccioso potencial биологической опасности

可能的传染性物潜在的な感染性物質 전염하는 물자

Fora (o poder) На (мощности)

开（电源）ン（電源） 에 (힘)

Fora de (poder)

С (сила)

（电源）

ン（電源） 떨어져 (힘)

Terra de proteção (terra) Защитное заземление (земля)

保护接地保護アース（接地） 방어적인 지구 (지상)

Terra Земли

接地アース（接地） 지구 (지상)

In vitro dispositivo médico diagnóstico В медицинской службе диагностики vitro

体外诊断医疗设备生体外の診断医療機器

생체외 진단 의료 기기

Page 52

English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어

Symbol Symbol Simbolo Symbole Simbolo

Simbole

символ

符号

記号

상징

Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목

Manufacturer Hersteller Fabricante Fabricant Fabbricante

Consult Instructions for Use Konsultieren Sie Anwendungsvorschriften Consulte las instrucciones para el uso Consultez les instructions pour l'usage Consulti le istruzioni per uso

Fabricante

производитель

制造商メーカー 제조자

Consulte instruções para o uso Советуйте с инструкциями для пользы

咨询使用说明书使用説明に相談しなさい 사용 설명을 상담하십시오

Page 53

Page 54

www.beckmancoulter.com

Beckman Coulter Allegra X-14 Instructions For Use Manual

Specifications and Main Features

Frequently Asked Questions

User Manual