Beckman Coulter Allegra X-12, Allegra X-12R Instructions For Use Manual

Instruction For Use

Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Allegra X-12 Series

Centrifuges

GX-IM-5AB
November 2013

Allegra X-12 Series
Centrifuges

GX-IM-5AB (November 2013)

Copyright © 2013 Beckman Coulter, Inc.

The following copyright statement is for position only. You
may replace it with a statement approved by your group.

All rights reserved. No part of this document may be
reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.

Find us on the World Wide Web at:

www.beckmancoulter.com

Made in USA

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.

Alerts for Danger, Warning, Caution, and Note

DANGER

DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.

Safety Notice

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.

NOTE

NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

Safety During Installation and/or Maintenance

This centrifuge weighs 121 kg (267 lb). Do not attempt to lift or move it without assistance.

Any servicing of this equipment that requires removal of any covers can expose parts that involve
the risk of electric shock or personal injury. Make sure that the power switch is off and the
centrifuge is disconnected from the main power source, and refer such servicing to qualified
personnel.

Do not replace any centrifuge components with parts not specified for use on this instrument.

GX-IM-5AB

iii

Safety Notice

Electrical Safety

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to
connect the centrifuge to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to
the centrifuge.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet

receptacle strip.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the centrifuge and damage electrical or mechanical components.

Safety Against Risk of Fire

This centrifuge is not designed for use with materials capable of developing flammable or explosive
vapors or hazardous chemical reactions. Do not centrifuge such materials (such as chloroform or
ethyl alcohol) in this centrifuge nor handle or store them within the 30-cm (1-ft) area surrounding
the centrifuge.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the rotors and accessories designed for use in this centrifuge.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop the rotor by hand.

• Do not lift or move the centrifuge while the rotor is spinning.

• NEVER attempt to override the door interlock system while the rotor is spinning.

• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running. During

operation you should come within the envelope only to adjust instrument controls, if
necessary. Never bring any flammable substances within the 30-cm (1-ft) area surrounding the
centrifuge. Never lean on the centrifuge or place items on the centrifuge while it is operating.

iv

GX-IM-5AB

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this centrifuge, however, unless all necessary

safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to their
use.

• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.

Safety Notice

Chemical and Biological Safety

It is your responsibility to decontaminate the centrifuge and accessories before requesting service
by Beckman Coulter.

GX-IM-5AB

v

Safety Notice

RoHS Notice

RoHS Notice

These labels and materials declaration table (the Table of Hazardous Substance's Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.

China RoHS Caution Label

This label indicates that the electronic information product contains certain toxic or hazardous
substances. The center number is the Environmentally Friendly Useful Period (EFUP) date, and
indicates the number of calendar years the product can be in operation. Upon the expiration of the
EFUP, the product must be immediately recycled. The circling arrows indicate the product is
recyclable. The date code on the label or product indicates the date of manufacture.

China RoHS Environmental Label

This label indicates that the electronic information product does not contain any toxic or hazardous
substances. The center ‘e’ indicates the product is environmentally safe and does not have an
Environmentally Friendly Useful Period (EFUP) date. Therefore, it can safely be used indefinitely.
The circling arrows indicate the product is recyclable. The date code on the label or product
indicates the date of manufacture.

vi

GX-IM-5AB

Safety Notice,iii

Alerts for Danger, Warning, Caution, and Note, iii

Safety During Installation and/or Maintenance, iii

Electrical Safety,iv

Safety Against Risk of Fire,iv

Mechanical Safety,iv

Chemical and Biological Safety,v

Contents

RoHS Notice,vi

Introduction,xiii

Certification,xiii

Scope of Manual,xiii

Typographic Conventions,xiv

CFC-Free Centrifugation,xiv

Radio Interference,xiv

Recycling Label,xv

CHAPTER 1: Description,1-1

Centrifuge Function and Safety Features,1-1

Centrifugation Function,1-1
Safety Features,1-2

Centrifuge Chassis,1-2

Housing,1-2
Door,1-2
Rotor Chamber,1-3
Temperature Sensing and Control,1-3
Drive,1-3

Controls and Indicators,1-3

Power Switch,1-3
Control Panel,1-3

vii

Contents

Name Rating Plate,1-6

Specifications,1-7

Available Rotors,1-9

CHAPTER 2: Installation,2-1

Introduction,2-1

Space and Location Requirements,2-1

Electrical Requirements,2-2

Securing the Centrifuge to the Floor,2-3

Test Run,2-3

CHAPTER 3: Operation,3-1

Introduction,3-1

Summary of Run Procedures,3-2

Manual Run,3-2
Programmed Run,3-3

Preparation,3-3

Installing the Rotor,3-3

Manual Operation,3-4

Selecting a Rotor,3-4
Entering Run Speed,3-5
Entering Run Time,3-5
Entering Run Temperature (Allegra X-12R only),3-6
Entering Acceleration and Deceleration Rates,3-6
Starting a Run,3-7
Pulse Function,3-8

Programmed Operation,3-8

Creating a New Program,3-8
Recalling a Program,3-9

Disabling/Enabling the Audible Tones,3-10

CHAPTER 4: Troubleshooting,4-1

Introduction,4-1

User Messages,4-1

Other Possible Problems,4-4

CHAPTER 5: Care and Maintenance,5-1

viii

Retrieving Your Sample In Case of Power Failure,4-5

Introduction,5-1

Instrument Care,5-1

Maintenance,5-1
Cleaning,5-2
Decontamination,5-3
Sterilization and Disinfection,5-3

Circuit Breaker and Fuses,5-3

Storage and Transport,5-4

Storage,5-4

Supply List,5-4

Replacement,5-4
Supplies,5-4

Beckman Coulter, Inc.
Allegra X-12 Series Centrifuge Warranty

Contents

ix

Illustrations

Illustrations

1.1 The Centrifuge,1-1

1.2 Control Panel,1-4

2.1 Centrifuge Dimensions,2-2

4.1 Door Latch Override,4-6

x

Tables

3.1 Acceleration and Deceleration Rates (in Minutes:Seconds) to
and from Maximum Speed,3-7

4.1 Diagnostic Message Chart,4-2

4.2 Troubleshooting Chart,4-5

Tables

xi

Tables

xii

Certification

To ensure full system quality, Beckman Coulter Allegra X-12 series centrifuges are manufactured in
a registered ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when
used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable
regulatory agencies. Declarations of conformity and certificates of compliance are available at
www.beckmancoulter.com.

Introduction

Scope of Manual

This manual is designed to familiarize you with the Beckman Coulter Allegra X-12 series
centrifuges, their functions, specifications, operation, and routine operator care and maintenance.
We recommend that you read this entire manual, especially the Safety Notice
information, before operating the centrifuge or performing instrument maintenance.

• CHAPTER 1 contains system specifications and a brief physical and functional description of the

centrifuge, including the operating controls and indicators.

• CHAPTER 2 provides requirements for preparing laboratory facilities and installing the

centrifuge.

• CHAPTER 3 contains centrifuge operating procedures.

• CHAPTER 4 lists diagnostic messages and other possible malfunctions, together with probable

causes and suggested corrective actions.

• CHAPTER 5 contains procedures for routine operator care and maintenance, as well as a brief

list of supplies and replacement parts.

NOTE

If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.

and all safety-related

GX-IM-5AB

xiii

Introduction

Typographic Conventions

Typographic Conventions

Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.

• Key names (for example,

CFC-Free Centrifugation

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of
Allegra X-12 series centrifuges.

Radio Interference

This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of FCC Rules. These limits are designed to provide reasonable protection against
harmful interferrence when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with this instruction manual may cause interference to radio communications.
Operation of this equipment in a residential area is likely to cause interference, in which case the
user will be required to correct the interference at his own expense.

Canadian Regulations

START or ROTOR) appear in boldface type.

This equipment does not exceed the Class A limits for radio noise emissions from digital apparatus
as set out in the radio interference regulations of the Canadian Department of Communications.

Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites
applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage
radioelectrique édicté par le Ministère des Communications du Canada.

xiv

GX-IM-5AB

Recycling Label

A28219-AA

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:

1. the device was put on the European market after August 13, 2005 and

2. the device is not to be disposed via the municipal waste collection system of any member state

of the European Union.

It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.

Introduction

Recycling Label

GX-IM-5AB

xv

Introduction

Recycling Label

xvi

GX-IM-5AB

Centrifuge Function and Safety Features

Centrifugation Function

The Beckman Coulter Allegra X-12R (refrigerated) or Allegra X-12 (constant controlled
temperature) centrifuge (see Figure 1.1) is a benchtop centrifuge that generates centrifugal forces
required for a wide variety of applications. Together with the Beckman Coulter rotors designed for
use in this centrifuge, the centrifuge applications include:

CHAPTER 1

Description

• Routine processing such as sample preparations, pelleting, extractions, purifications,
concentrations, phase separations, receptor binding, and column centrifugations.

• Cell isolation.

• Binding studies and separation of whole blood.

• Processing large numbers of small-volume samples in multiwell plates for concentrating tissue-

culture cells, cloning and replicate studies, in-vitro cytotoxicity studies, receptor binding, and
genetic engineering experimentation.

• Rapid sedimentation of protein precipitates, large particles, and cell debris.

Figure 1.1 The Centrifuge

The Allegra X-12 and X-12R are microprocessor-controlled, providing interactive operation. The
instrument design features a brushless asynchronous motor, automatic rotor identification system,

GX-IM-5AB

1-1

Description

Centrifuge Chassis

program memory that enables repeated run conditions, temperature control system, and a choice
of acceleration and deceleration rates. User messages alert the operator to conditions that may
need attention.

Safety Features

Allegra X-12 series centrifuges have been designed and tested to operate safely indoors at altitudes
up to 2 000 m (6 562 ft).

Safety features include the following.

• An electromechanical door lock system prevents operator contact with spinning rotors and
prevents run initiation unless the door is shut and locked. The door is locked when a run is in
progress and can be opened only when the rotor is stopped and the LED (light-emitting diode)
next to the
sample recovery.

• A steel barrier surrounds the rotor chamber to provide full operator protection.

• A rotor identification system prevents the installed rotor from running above its maximum

rated speed. During acceleration the microprocessor checks the magnetic rotor identification.
Speed is limited to the maximum safe speed of the identified rotor. If the system identifies a
rotor different than the one entered by the user, the system displays an error message and
reduces the speed to the maximum allowable speed of the rotor.

DOOR key is lit. If there is a power failure, the door lock can be manually tripped for

• An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor
loads are severely out of balance. At low speeds, an incorrectly loaded rotor can cause
imbalance. Rotor instability can also occur if the centrifuge is moved while running, or if it is
not resting on a level and secure surface (see CHAPTER 4, Troubleshooting).

Centrifuge Chassis

Housing

The centrifuge housing is made of sheet steel and structural foam and is finished with acrylic baking
enamel. The control panel overlay is made of coated polystyrene copolymer.

Door

The aluminum and structural foam door is secured to the housing by solid hinges. A window in the
center allows strobe viewing. When the door is closed, the locking system engages. The door is
locked when a run is in progress and can be opened only when the rotor is stopped and the LED next
to the

DOOR key is lit. If there is a power failure, the door lock can be manually tripped for sample

recovery (see CHAPTER 4, Troubleshooting).

1-2

GX-IM-5AB

Rotor Chamber

The aluminum rotor chamber is coated with epoxy enamel for corrosion resistance. The chamber
is sealed by a foam gasket.

Temperature Sensing and Control

With the power on, the temperature control system is activated when the door is closed and locked.
A sensor in the rotor chamber continuously monitors chamber temperature. The microprocessor
calculates the required chamber temperature to maintain the selected rotor temperature. Run
temperature in the Allegra X-12R can be set between –10 and +40°C. Temperature in the
Allegra X-12 is factory set at 20°C, and is maintained at between 18 and 22°C to prevent sample
heating.

Description

Controls and Indicators

1

NOTE

To avoid chamber icing, refrigeration is off when the door is open. The centrifuge door must be closed

and locked for the refrigeration system to begin operating.

Drive

The asynchronous direct-drive motor is brushless for clean, quiet operation. The resilient
suspension ensures that loads will not be disturbed by vibration, and prevents damage to the drive
shaft if an imbalance occurs during centrifugation. Maximum acceleration and deceleration may be
selected to allow fast processing of samples; alternately, delicate gradients may be preserved using
slower acceleration and deceleration.

Controls and Indicators

Power Switch

The power switch, located on the left side of the centrifuge, controls electrical power to the
centrifuge. It is also a circuit breaker that will trip to cut off power in the event of a power overload.
The power switch must be turned on before the chamber door can be opened.

Control Panel

The control panel (see Figure 1.2) is mounted at an angle on the centrifuge front for easy visibility
and access. It is used to enter run parameters via function keys and a keypad, and to display run
parameters, program information, and user messages.

GX-IM-5AB

1-3

Description

PULSE

ENTER/

SAVE

Controls and Indicators

Figure 1.2 Control Panel

Digital Displays

The displays provide run information, user messages, and diagnostics.

• During a run, they show the actual (real-time) operating conditions.

• When the run parameters are being entered, they show the set values selected. Set values can

be recalled during operation by pressing
actual conditions.

SHOW SET. After 5 seconds, the displays return to

• Error messages (see CHAPTER 4) also appear on the displays, when applicable. The centrifuge
emits a series of audible tones to alert the user to an error condition. (Instructions for disabling
the tones are in CHAPTER 3.)

SPEED Indicates rotor speed in revolutions per minute (rpm) or in relative centrifugal field, a

term describing the ratio of the centrifugal acceleration at a specified radius and speed
to the standard acceleration of gravity (RCF ´ g).

TIME • In a timed run, indicates the remaining run time in hours and minutes. The time

display begins counting down when ENTER/SAVE then START is pressed and
continues counting down to 0. The run automatically ends when the set time
reaches 0 and deceleration begins (a tone sounds when the rotor stops spinning).
If a malfunction shuts down the centrifuge during a run, when the instrument is
turned back on the time will not remain and 00:00 will be displayed in the time
window.

• In a hold run, indicates the time elapsed since START was pressed. After 99 hours

and 59 minutes the timer resets to 0 and continues counting elapsed time.

• In a pulse (momentary) run, indicates the time (in minutes and seconds) elapsed

since PULSE was pressed. Time continues to count up until the PULSE key is
released. The total elapsed time is then displayed until another key is pressed.

TEMPERATURE Indicates rotor temperature in degrees C at thermoequilibrium.

ROTOR Displays the name of the selected rotor.

ACCEL When the ACCEL key is pressed, one of ten available acceleration rates can be entered.

Displays the selected acceleration rate throughout the run.

1-4

GX-IM-5AB

Description

Controls and Indicators

DECEL When the DECEL key is pressed, one of eleven available deceleration rates can be

entered. Displays the selected deceleration rate throughout the run.

PROGRAM • During setup—when the PROGRAM key is pressed, a program number (one of ten

possible) can be selected and program parameters entered.

• During centrifugation — the number of the program being run is displayed.

Run-Parameter Keys

Run-parameter keys are used to enter specific run information as follows. The centrifuge emits an
audible tone when each key is pressed. (Instructions for disabling the tone are in CHAPTER 3).

Pressed to display scrollable list of compatible rotors. Can be pressed repeatedly to
scroll through the rotor list (or the arrow keys can be used for scrolling).

If the display shows speed in rpm, the key is pressed once to enter run speed (using the
keypad) in rpm (revolutions per minute); pressed twice to enter run speed in RCF
(relative centrifugal field). If the display shows speed in RCF, the key is pressed once to
enter speed in RCF or twice to enter speed in rpm.

1

Pressed to enter run time using the keypad. When TIME is pressed twice, HOLD mode
is selected. (Pressing it again reverts to time-entry mode.)

• Timed run—Run time up to 99 hours and 59 minutes can be set. If more than

59 minutes are entered in the minutes field, the system automatically converts
the entry to hours and minutes. Deceleration begins when the set time counts
down to 0.

• Continuous run (hold)— For runs of unspecified lengths, HOLD mode is used. When

99 hours, 59 minutes is reached, the system resets to 0 and continues counting.
A hold run continues until the STOP key is pressed.

Allegra X-12R only—Pressed to enter run temperature (using the keypad), from –10 to
+40°C. If a new temperature is not entered, the centrifuge uses the temperature set for
the previous run.

Enables selection of one of ten preset acceleration rates (see Table 3.1 in CHAPTER 3)
available to protect specific gradient and sample-to-gradient interfaces. If
no acceleration rate is selected, the centrifuge defaults to maximum acceleration.

Enables selection of one of eleven preset deceleration rates (see Table 3.1 in

CHAPTER 3) available to maintain optimum separation. If no deceleration rate is

selected, the centrifuge defaults to maximum deceleration. (Deceleration rate 0 is
always coast to complete stop.)

A toggle switch used to enter into and exit out of the program mode to create or recall
programs. When the key is pressed, the program number can be selected using the
keypad or arrow keys.

GX-IM-5AB

1-5

Description

Name Rating Plate

Up and down arrow keys, which can be pressed to increment parameter values up or
down.

The ENTER/SAVE key can be pressed to save parameter inputs to system memory
during manual run setup or to a program during a program set up, and to start the
centrifuge (ENTER/SAVE, then START must be pressed to start the centrifuge).

The keypad is used to enter numerical run parameters, to enter or recall a program
number, and to select acceleration and/or deceleration rates. In addition to keys
0 through 9, the keypad includes a plus/minus key and a CE (clear entry) key.

The ± key is used to enter a temperature setting below 0°C (for example, –2°C) in the
Allegra X-12R.

The CE (clear entry) key clears any parameter on the display. Pressing CE will also clear
some diagnostic messages. If a diagnostic message does not clear when CE is pressed,
see CHAPTER 4, Troubleshooting.

Name Rating Plate

Pressed during a run to display set values for 5 seconds.

Causes the rotor to accelerate, at maximum rate, to set speed for short-duration runs
(as long as the key is pressed). Deceleration, at maximum rate, begins when the key is
released.

Pressing ENTER/SAVE, then START begins the run. (START must be pressed within
5 seconds of pressing ENTER/SAVE.) This key combination can also be used to abort a
deceleration process and restart the centrifuge.

Can be pressed at any time while the rotor is spinning to terminate a run. The centrifuge
decelerates to a complete stop according to the deceleration setting. The green light
next to the key flashes while the rotor decelerates. A tone sounds when the rotor comes
to a complete stop. (Instructions for disabling the tone are in CHAPTER 3.)

Unlocks and opens the door. (The instrument will not accept the unlock command if the
rotor is spinning.)

A name rating plate is affixed to the rear of the instrument. Always mention the serial number and
model number when contacting Beckman Coulter regarding your centrifuge.

1-6

GX-IM-5AB

Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.

Specification Description

Speed • Set speed

Time • Set time

Temperature • Set Temperature

Humidity restrictions <75% (noncondensing)

Description

Specifications

— 200 to 10 200 rpm in 10-rpm increments

• Speed display

— actual rotor speed in 10-rpm increments or in RCF (when

selected)

— to 99 hours 59 minutes or continuous (hold)

• Time display
— Timed run

· indicates run time remaining

— Continuous (hold) or pulse run

· indicates elapsed time

— Allegra X-12R is –10 to +40°C in 1° increments

— Allegra X-12 is factory set at 20°C

• Temperature control (after equilibration)

— Allegra X-12R is ±2°C of set temperature
— Allegra X-12 is ±2°C of the 20°C set temperature

• Temperature display (after equilibration)
— chamber temperature in 1° increments

• Ambient temperature range
— 10 to 35°C

• Ambient temperature range for optimum operation
— 10 to 25°C

a

1

Acceleration 10 acceleration rates

Deceleration 11 deceleration rates

Dimensions • Width

Weight 121 kg (267 lb)

Ventilation clearances (sides and rear) 7.6 cm (3.0 in.)

GX-IM-5AB

— 76.2 cm (30.0 in.)

• Depth

— 62.2 cm (24.5 in.)

• Height

— 34.3 cm (13.5 in.)

1-7

Description

Specifications

Specification Description

Fin ish es • Control panel

— coated polystyrene copolymer

• Housing surfaces

— acrylic baking enamel

Electrical requirements • 208-V, 60-Hz instrument

— 187–229 VAC, 9 A, 60 Hz

• 200-V, 50/60-Hz instrument
— 180–220 VAC, 10 A, 50/60 Hz

• 230-V, 50-Hz instrument
— 207–253 VAC, 8 A, 50 Hz

Electrical supply Class I

Maximum heat dissipation into room

4100 Btu/hr (1.2 kW)

under steady-state conditions

Noise level 0.91 m (3 ft) in front of

£ 68 dBa

centrifuge

Installation (overvoltage) category II

Pollution degree

a. During transient conditions, such as acceleration and deceleration, rotor temperature may be outside this range. To reach

temperatures above ambient, the centrifuge is dependent on the frictional heat generated inside the chamber during
operation. At low run speeds or low ambient temperatures, the centrifuge may not be able to achieve some higher
temperatures.

b. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation

must be expected.

b

2

1-8

GX-IM-5AB

Available Rotors

The following Beckman Coulter rotors can be used in the Allegra X-12 series centrifuge. The rotors
are described in individual manuals that accompany each rotor.

Description

Available Rotors

1

Rotor Profile Description

FX6100 Fixed Angle

r

= 98.0 mm

max

SX4750 Swinging Bucket

Tube-and-bottle buckets,

r

= 207.8 mm

max

Multiwell-plate carriers,

= 183.2 mm

r

max

SX4750A Swinging Bucket
(ARIES)

Tube-and-bottle buckets,

r

= 207.8 mm

max

Multiwell-plate carriers,

= 183.2 mm

r

max

Rotor

Selection

Code

Max

RPM

a

Max

b

RCF

(´ g) at

r

max

Number

of Tubes ´

Nominal

Capacity

Rotor

Manual

Number

FX6100 10 200 11 400 6 ´ 100 mL GX-TB-005

GX-TB-003

SX4750

SX4750m

3 750

3 750

3 270

2 885

4 ´ 750 mL

4 ´ 96 mL

GX-TB-004

SX4750A

SX4750A

3 750

3 750

3 270

2 885

4 ´ 750 mL

4 ´ 96 mL

a. Maximum speeds are based on a solution density of 1.2 g/mL. At upper temperature and humidity ambient conditions, swinging bucket rotor

speed may require reduction.

b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rω

gravity (g) according to the following formula: RCF = r ω

(2 π RPM/60), and g is the standard acceleration of gravity (9807 mm/s

GX-IM-5AB

2

2

/g — where r is the radius in millimeters, ω is the angular velocity in radians per second

2

). After substitution: RCF = 1.12 r (RPM/100)2.

) to the standard acceleration of

1-9

Description

Available Rotors

1-10

GX-IM-5AB

Introduction

Preinstallation requirements have been sent prior to shipment of your centrifuge.

CHAPTER 2

Installation

WARNING

This centrifuge weighs 121 kg (267 lb). Do not attempt to lift or move it without
assistance.

Space and Location Requirements

WARNING

Do not place the centrifuge near areas containing flammable reagents or
combustible fluids. Vapors from these materials could enter the centrifuge air
system and be ignited by the motor.

The centrifuge ships in a cardboard box on a wooden pallet. For easy access, remove the top of the
box, the foam insert on top of the centrifuge, and then the upper part (sides) of the box and set them
aside. Then, with the help of another person, move the centrifuge from the pallet to its final position.

1

Position the centrifuge on a level surface, such as a sturdy table or laboratory bench that is able
to support the weight of the centrifuge (121 kg/267 lb) and resist vibration.

a. Make sure that the centrifuge front feet are fully supported on the table.

2

Locate the centrifuge in an area with sufficient ventilation to allow for heat dissipation.

3

Check that there are 7.6-cm (3-in.) clearances at the sides and back of the centrifuge to ensure
sufficient air circulation. Dimensions are shown in Figure 2.1.

Additional clearance is required on the left side to allow access to the power switch.

GX-IM-5AB

2-1

Installation

cm

in.

96.5

38.0

76.2

30.0

7.6

3.0

7.6

3.0

34.3

13.5

62.2

24.5

Electrical Requirements

Ambient temperatures during operation should not be lower than 10°C (50°F) or higher than
35°C (95°C). Relative humidity should not exceed 75% (noncondensing).

The centrifuge must have adequate air ventilation to ensure conformance to local
requirements for vapors produced during operation.

Figure 2.1 Centrifuge Dimensions

Electrical Requirements

208-V, 60-Hz centrifuge 187–229 VAC, 9 A, 60 Hz

200-V, 50/60-Hz centrifuge 180–220 VAC, 10 A, 50/60 Hz

230-V, 50-Hz centrifuge 207–253 VAC, 8 A, 50 Hz

To reduce the risk of electrical shock, this centrifuge comes with a 2.5-m (8-ft) three-wire electrical
cord (attached to the power connector at the rear of the instrument) and plug to connect the

2-2

centrifuge to earth-ground. The plug for use with 208-VAC, 60-Hz centrifuges is the NEMA 6-15P;
a NEMA 6-15R socket should be available. In regions where the instrument is supplied with an
unterminated cord, a plug that meets local electrical and safety requirements must be supplied.
(Contact your local Beckman Coulter Service Office for specific information regarding these
requirements.)

GX-IM-5AB

Installation

Securing the Centrifuge to the Floor

1

Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.

a. Check that the line voltage agrees with the voltage listed on the name rating plate affixed

to the centrifuge.

2

Never use a three-to-two wire plug adapter.

3

Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.

4

If there is any question about voltage, have a qualified service person measure it under load
while the drive is operating.

5

To ensure safety, the centrifuge should be wired to a remote emergency switch (preferably
outside the room where the centrifuge is housed, or adjacent to the exit from that room) in

order to disconnect the centrifuge from the main power source in case of a malfunction.

2

Securing the Centrifuge to the Floor

Allegra X-12 series centrifuges are certified to meet the requirements of the European CE mark. To
meet these requirements, the centrifuge must be secured to the bench using the anchoring kit
shipped with the instrument. This will prevent the centrifuge from moving in the unlikely event of
a rotor mishap.

Complete instructions for installing the anchoring kit are packaged with the hardware, which is
shipped with the centrifuge. The instructions (publication GX-TB-002) include a full-size template
to be used as a guide for drilling holes in the bench. Refer to this document for installation
instructions.

Test Run

NOTE The centrifuge must be plugged in and the power switch turned to on position (I) before the door

can be opened.

We recommend that you make a test run to ensure that the centrifuge is in proper operating
condition following shipment. See Section 3 for instructions on operating the centrifuge.

After completing the test run, return the pre-addressed warranty card included with this literature.
This will validate the centrifuge warranty and ensure your receipt of further information regarding
new accessories and/or modifications as they become available.

GX-IM-5AB

2-3

Installation

Test Run

2-4

GX-IM-5AB

Introduction

This section contains centrifuge operating procedures. A summary is provided at the start of this section.
If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of
operating steps. Refer to the applicable rotor manual for instructions on preparing the rotor for
centrifugation.

CHAPTER 3

Operation

WARNING

Handle body fluids with care because they can transmit disease. No known test
offers complete assurance that they are free of micro-organisms. Some of the
most virulent—Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria,
and certain systemic fungi— further emphasize the need for aerosol protection.
Handle other infectious samples according to good laboratory procedures and
methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic,
pathogenic, or radioactive materials in this centrifuge without taking appropriate
safety precautions. Biosafe containment should be used when Risk Group II
materials (as identified in the World Health Organization Laboratory Biosafety
Manual) are handled; materials of a higher group require more than one level
of protection.

Do not use the centrifuge in the vicinity of flammable liquids or vapors, and do not
run such materials in the instrument. Do not lean on the centrifuge or place items
on it while it is operating.

GX-IM-5AB

3-1

Operation

Summary of Run Procedures

Summary of Run Procedures

For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration.

Manual Run

1

Turn the power switch on (I).

2

Press DOOR to open the chamber door; lift the door open.

3

Install the rotor according to the applicable rotor manual, then close the chamber door.

4

Press ROTOR, then press ROTOR again or use the arrow keys to select the rotor.

5

Press RPM/RCF, then use the keypad to enter the run speed. Press RPM/RCF a second time to
toggle between RPM and RCF modes.

6

Press TIME, then use the keypad to enter the run time (to 99 hrs, 59 minutes); or press TIME
twice to toggle between hold (continuous) run and set time mode.

7

Press TEMP °C, then use the keypad to enter the required run temperature –10 to +40°C
(Allegra X-12R only).

8

Press ACCEL, then use the keypad or arrow keys to enter the selected acceleration rate number,

1 (SLOW) through 10 (MAX).

9

Press DECEL, then use the keypad or arrow keys to enter the selected deceleration rate number,

0 (OFF) through 10 (MAX).

10

Check that all parameters are correct and that the door is closed (door LED will be lit).

ENTER/SAVE, then press START (within 5 seconds).

Press

11

Wait for the set time to count down to zero, or end the run by pressing STOP.

12

When the rotor stops (a tone sounds), press DOOR to open the chamber door; lift the door.

3-2

GX-IM-5AB

Programmed Run

1

Turn the power switch on (I).

2

Press DOOR to open the chamber door.

3

Install the rotor according to the applicable rotor manual, then close the chamber door.

4

Press PROGRAM, use the keypad or arrow keys to enter the required program number, then
press

5

Check that all parameters are correct and that the door is closed (door LED will be lit).
Press

ENTER/SAVE.

ENTER/SAVE, then press START (within 5 seconds).

Operation

Preparation

3

6

Wait for the run to end, or end the run by pressing STOP.

7

When the rotor stops (a tone sounds), press DOOR to open the chamber door; lift the door.

Preparation

Prepare the rotor for centrifugation as described in the applicable rotor manual. For runs at other than
room temperature, refrigerate or warm the rotor beforehand for fast equilibration.

Installing the Rotor

The power must be turned on before you can unlock and open the chamber door. (To end a run for
any reason, do not turn the power switch off; press

1

Turn the power switch on.

• The control panel display shows “

STOP instead.)

Allegra X-12R (or Allegra X-12)” and the software version.

2

Press DOOR.

• The door opens.
— The centrifuge will accept this command only when the rotor is at rest.

GX-IM-5AB

3-3

Operation

Manual Operation

3

Install the rotor according to directions in the rotor manual.

a. Ensure that the rotor is seated on the drive hub.
b. Avoid bumping the control panel keys during rotor installation or removal.

4

Close the chamber door by pressing down firmly on both sides.

• The door locks.

• When the latches engage, the LED next to the DOOR key lights up.

Manual Operation

NOTE When using multiwell-plate carriers, enter SX4750A as the rotor and limit top speed of the instrument

to 3750 rpm in an Allegra X-12 series centrifuge or 4450 rpm in an Allegra X-15R (when running up to
three stacked Beckman Coulter plates separated by cap strips; reduce speed to 2700 rpm if running four
stacked plates). Set rotor speed using rpm values only. To set speed to a specific RCF, use the rpm-to-RCF
conversion chart (GX-TB-015) to determine the applicable rpm to enter.

1

When a run-parameter key (ROTOR, RPM/RCF, TIME, TEMP, ACCEL, or DECEL) is pressed, a
parameter can be entered or changed.

• Entry is completed when

2

To change an entry before you’ve pressed ENTER/SAVE or another parameter key, enter a
different value.

a. Press
b. To change an entry after you’ve pressed

3

If an unacceptable value is entered, an out of range message is displayed in the message line.

a. Enter the correct value.

Selecting a Rotor

1

Press ROTOR, then use the arrow keys or press ROTOR again to select a rotor.

ENTER/SAVE or another function key is pressed.

CE to abort changes and start over.

ENTER/SAVE, press the run-parameter key again.

2

Press the next run-parameter key or press ENTER/SAVE.

3-4

GX-IM-5AB

Entering Run Speed

Enter a run speed up to the maximum speed of the rotor in use. Or, enter a relative centrifugal field
(RCF) value up to the maximum achievable RCF of the rotor.

1

Press RPM/RCF once to enter speed in RPM.

• Allowed speed range for the selected rotor is displayed.

Operation

Manual Operation

3

a. Press

2

Use the keypad to enter required speed.

• Entered speed appears on the speed display.
— (If the entered speed is out of the speed range of the installed rotor, an error message

a. Enter an acceptable value from the range displayed.)

3

Press the next run-parameter key or ENTER/SAVE.

• The rotor will accelerate or decelerate to the new speed.

a. You can change the set speed at any time during a run by repeating steps 1 through 3.

Entering Run Time

Run time can be set for up to 99 hours and 59 minutes or for a hold (continuous) run. The time
display begins counting down when the rotor starts to spin. For a timed run, the run automatically
terminates when the set time reaches zero and deceleration begins at the selected rate; a tone
sounds when the rotor has stopped. For a hold run, the elapsed time is displayed and the run
continues until

RPM/RCF again to toggle between RPM and RCF modes.

will appear and the input will be cleared.

STOP is pressed.

1

Press TIME.

HOLD is displayed, press TIME again to enter time.

a. If

2

Use the keypad or arrow keys to enter required time.

• Entered time appears on the display.
— If you entered a number higher than 59 minutes, the centrifuge automatically

recalculates the time in hours and minutes.

or

a. Press

GX-IM-5AB

TIME again for a hold run.

•

HOLD appears on the display.

3-5

Operation

Manual Operation

3

Press the next run-parameter key or ENTER/SAVE.

Entering Run Temperature (Allegra X-12R only)

NOTE Temperature is factory set at 20°C on the Allegra X-12.

Run temperature can be set from –10 to +40°C. If no value is entered, the centrifuge selects the last
entered temperature.

1

Press TEMP.

• The cursor and the temperature range (–10 to 40°C) appear.

2

Use the keypad or arrow keys to enter required temperature.

• Entered temperature appears on the display.
— (If the entered temperature is outside the valid temperature range, an error message

will appear and the input will be cleared.

a. Enter a valid temperature.)

3

Press the next run-parameter key or ENTER/SAVE.

Entering Acceleration and Deceleration Rates

You can select one of ten acceleration rates and eleven deceleration rates to protect the gradient
and sample-to-gradient interface. Select rates depending on the type of run you are performing—

for pelleting runs, where sample mixing is not a concern, maximum acceleration and deceleration
can be used. If running delicate gradients, a lower setting may be needed. Typical acceleration and
deceleration rates are listed in Table 3.1. If no rate is selected, the centrifuge automatically
accelerates and decelerates at maximum.

1

Press ACCEL, then use the keypad or arrow keys to select the required acceleration rate.

• Entered acceleration rate appears on the display.

2

Press the next run-parameter key or ENTER/SAVE.

3

Press DECEL, then use the keypad or arrow keys to select the required deceleration rate.

• Entered deceleration rate appears on the display.

3-6

GX-IM-5AB

4

Press the next run-parameter key or ENTER/SAVE.

Operation

Manual Operation

3

Table 3.1 Acceleration and Deceleration Rates (in Minutes:Seconds) to and from Maximum Speed

SX4750 SX4750m SX4750A FX6100

(3 750 RPM) (3 750 RPM) (3 750 RPM) (10 200 RPM)

Rate

10 (MAX)

9
8
7

6
5
4
3

2

1 (SLOW)

0 (OFF)

a. Times shown are with the rotor fully loaded in the Allegra X-12R. (Times are approximate; actual times will vary depending

on the rotor in use, the rotor load, run speed, and voltage fluctuations.

Accel Decel Accel Decel Accel Decel Accel Decel

1:21
1:51
2:00
2:12

2:28
2:47
3:12
3:49

4:43
6:15

—

1:15
1:55
2:06
2:17

2:29
2:49
3:17
3:53

4:49
6:21

coast

1:16
1:51
2:02
2:23

3:04
3:18
4:24
4:34

4:59
6:54

—

1:09
1:52
2:04
2:13

2:26
2:47
3:16
3:52

4:47
6:24

coast

1:19
1:51
2:01
2:13

2:55
3:11
3:46
4:37

6:11
6:15

—

1:15
1:52
2:03
2:17

2:28
2:48
3:14
3:53

4:49
6:20

coast

1:33
2:30
3:36
4:52

5:12
6:05
6:59
8:33

8:49

10:11

—

a

1:23
2:22
3:14
4:09

5:08
6:03
7:02
7:54

8:50
10:18
coast

Starting a Run

1

Press ENTER/SAVE and START.

• The light next to the

NOTE

The run will end when the time display counts down to zero.

a. (Press

2

After the rotor has stopped and the LED lights, press DOOR.

• The door opens.

START key flashes and the rotor begins to spin.

To begin a run, ENTER/SAVE must always be the last key pressed before pressing START. If you

wait more than 5 seconds, the

START again to begin the run.

STOP to terminate a run for any reason.)

• The green light next to the

START key will not activate. If this happens, press ENTER/SAVE and

STOP key will flash while the rotor decelerates.

• A tone sounds when the rotor comes to a complete stop.
— (Instructions for disabling the tone are at the end of this section.)

GX-IM-5AB

3-7

Operation

Programmed Operation

3

Remove the rotor (or containers).

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply appropriate decontamination procedures to the
centrifuge and accessories.

NOTE

Pulse Function

The pulse function, accessed by pressing the PULSE key, is used for short-duration runs as follows.

• When the
continues to spin as long as the
deceleration settings are overridden by the pulse function.) When the
the rotor begins decelerating to 0 rpm using maximum deceleration. Using the pulse function
eliminates the need to press the

• When the
the

• The centrifuge memory retains the parameters of the last run performed before the
was pressed. At the end of a pulse run, after the centrifuge door is opened and closed the
previous run parameters are displayed.

Depending upon the duration of the event, transient power-line interruptions may cause this
equipment to decelerate or reset with the possible loss of in-process operation. If you suspect this
has occurred, simply repeat the runs.

PULSE key is pressed, the rotor accelerates at maximum rate to the set speed and

PULSE key is pressed. (The current run time, acceleration, and

PULSE key is released,

START and STOP keys.

PULSE key is pressed, the TIME display begins displaying the elapsed seconds. When

PULSE key is released, the seconds stop accumulating.

PULSE key

Programmed Operation

The instrument internal memory can store up to 10 programs which can be recalled by keypad
numbers 0 through 9. Saved programs are retained in memory even if the power is turned off.
Procedures for entering run parameters are the same for programmed operation as those described
above for manual operation.

Creating a New Program

1

Press PROGRAM, then use the keypad or arrow keys to select a program number that is not in
use, or one that you want to overwrite, and press

• The program number flashes.

3-8

ENTER/SAVE.

GX-IM-5AB

Programmed Operation

2

Enter run parameters (rotor, speed, time, temperature, acceleration/deceleration rate
settings).

a. Press

b. (Press CE and enter an acceptable value, then press the next run-parameter key.)

3

When all parameters are entered, press ENTER/SAVE.

• The program is saved into memory and “Program saved” appears on the display.

• The program number displayed will stop flashing.

• If any parameters are then modified, the program number will show a dash.

4

Press ENTER/SAVE and START to run the programmed run.

ENTER/SAVE.

• If you entered an unacceptable parameter, an error message will be displayed.

Operation

3

NOTE

If the PROGRAM key is pressed while the program number is flashing, program mode is exited

without saving entered parameters into program memory.

Recalling a Program

1

Press PROGRAM, then use the keypad or arrow keys to enter the number of the program.

• The program parameters are displayed if

a. Press

2

Press ENTER/SAVE and START to begin the programmed run.

• The run starts, the green light next to the

NOTE

• The run will end automatically when the time display counts down to zero.

To begin a run, ENTER/SAVE must always be the last key pressed before pressing START. If you

wait more than 5 seconds, the

START again to begin the run.

ENTER/SAVE was pressed.

ENTER/SAVE to recall a program or press PROGRAM to exit program mode.

START key flashes, and the rotor begins to spin.

START key will not activate. If this happens, press ENTER/SAVE and

a. (Press

GX-IM-5AB

STOP to terminate a run at any time.)

• The green light next to the

• A tone will sound when the rotor has come to a complete stop.

STOP key will flash while the rotor decelerates.

3-9

Operation

Disabling/Enabling the Audible Tones

3

After the rotor has stopped and the LED is lit, press DOOR.

• The door opens.

4

Remove the rotor (or containers).

Disabling/Enabling the Audible Tones

The audible tones (beeps) that sound when a run-parameter key is pressed, or at the end of each
run, or if an error occurs may be turned on and off by following the steps below (rotor must be
at 0 rpm).

1

To disable the key or error tone press ENTER, then press 2 3 6.

• The words “

2

To disable the end-of-run tone, press ENTER, then press 2 2 3.

• The words “Disable RUN beep” appear on the display.

Tones that have been disabled can be enabled as follows.

1

To enable a key or error tone press ENTER, then press 2 3 5.

• The words “

2

To enable the end-of-run tone press ENTER, then press 2 2 2.

• The words “Enable RUN beep” appear on the display.

Disable KEY beep” appear on the display.

Enable KEY beep” appear on the display.

3-10

GX-IM-5AB

Introduction

This section lists possible malfunctions, together with probable causes and corrective actions.
Maintenance procedures are given in CHAPTER 5. For any problems not covered here, contact
Beckman Coulter Field Service (1-800-742-2345 in the United States; worldwide offices are listed on the
inside back cover of this manual) for assistance.

CHAPTER 4

Troubleshooting

NOTE

It is your responsibility to decontaminate the instrument, as well as any rotors and/or accessories,

before requesting service by Beckman Coulter Field Service.

User Messages

User messages appear on the display to communicate information about the instrument or to alert
you to abnormal conditions that need attention.

• Help and informational messages caused by incorrect input or certain operating conditions can
be cleared by pressing

• Diagnostic messages that result from abnormal operating conditions or equipment
malfunction require troubleshooting. Refer to Table 4.1 to determine the nature of the
condition and any recommended actions. If a problem persists after you have performed the
recommended action, call Beckman Coulter Field Service (in the United States, call 1-800-742­2345; outside the U.S., call your local Beckman Coulter office). To help the field service
representative diagnose and correct the problem, gather as much information about the
situation as you can, including:

— the diagnostic number and message,
— the operating situation when the diagnostic condition occurred (such as rotor in use,

— any unusual environmental and/or operating conditions (such as ambient temperature or

CE and following the instructions in the message.

speed, or load type), and

voltage fluctuations).

GX-IM-5AB

4-1

Troubleshooting

User Messages

Table 4.1 Diagnostic Message Chart

Diagnostic Number/

Message

Problem Result Recommended Action

P1–
Power failure
occurred, see manual

L1 through L6–
Door latch, see
manual

L7–
Can’t open door, see
manual

C1–
Rotor temp exceeds
4C above set

C2–
Rotor temp exceeds
8C above set, call
Service

System or facility
power failure

Latches are not
operating properly

Door latches do not
release

Rotor temperature
exceeds temperature
setting by more than
4°C but less than 8°C
after equilibration

Rotor temperature
exceeds temperature
setting by more than
8°C after equilibration

Run shuts down Press CE to clear message. Restart run.

Remaining time shows on display.

Error message
appears; run shuts
down with no brake

Press down on both sides of the door until the
DOOR LED comes on. Press CE

to clear

message. If you close the door repeatedly and
the problem continues, call Beckman Coulter
Field Service.

Door doesn’t open
when DOOR

key is

pressed

Press down on the door, then press DOOR
again. If door still doesn’t open call
Beckman Coulter Field Service. (Procedures
for retrieving your sample are at the end of
this section.) If the door opens but the
message stays on the display, press CE
clear.

Run continues; the

Press CE

to clear message.

message is
informational

Run shuts down

Call Beckman Coulter Field Service.

with no brake

to

C3 and C5–
Temp error, call

Refrigeration system
error

Service

T1 and T2–
Temp, door locks for

System temperature

problem
45 minutes, call
Service

T3–

Motor overheated Run shuts down
Temp, door locks for
45 minutes, see
manual

4-2

Run shuts down
with maximum
brake

Run shuts down
with no brake; door
locks for
45 minutes

with no brake; door
locks for
45 minutes

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Wait 45 minutes for motor to cool down, then
restart run. Press CE

to clear message. If

problem persists, call Beckman Coulter Field
Service.

GX-IM-5AB

Table 4.1 Diagnostic Message Chart (Continued)

Diagnostic Number/

Message

Problem Result Recommended Action

Troubleshooting

User Messages

4

D1 through D9–
Drive error, see
manual

R1 and R2–
Rotor ID problem,
see manual

R3–

Rotor ID mismatch,
check rotor

R4 and R5–
No rotor match, see
manual

Drive system problem Run stops, usually

with no brake

No magnets identified,
or magnets incorrectly
identified

The entered rotor
number is not the
same as the rotor
identified

The system cannot
identify the rotor

Run shuts down
with maximum
brake

Run shuts down
with maximum
brake

Run shuts down
with maximum
brake

After run stops press CE to clear message,
then restart run. If problem repeats, call
Beckman Coulter Field Service. Before trying
to open the door, listen carefully and make
sure that no sound is coming from the
chamber (indicating a spinning rotor). Follow
the directions under Retrieving Your Sample

In Case of Power Failure, below.

Check that magnets are in place on rotor
bottom. Press CE
repeats, call Beckman Coulter Field Service.

Press CE to clear message. Enter the correct
rotor entry identification.

• Make sure the rotor in use is a compatible

Beckman Coulter rotor (see Available

Rotors

magnets are in place on rotor bottom.
Press CE

• Call Beckman Coulter Field Service.

to clear message. If problem

in CHAPTER 1). Check that

to clear the message.

S1–

System error, call
Service

S8–

System error, door
locks for 45 minutes,
see manual

H1, H2, H4–
Speed error, call
Service

H3, H5, and H6–
Speed error, door
locks for 45 minutes,
call Service

There is a problem
with the system
control software,
EPROM, or RAM

There is a problem
with system control
software

Speed control problem Run shuts down

Speed control problem Run shuts down

System shuts
down, no brake

Run shuts down
with no brake; door
locks for
45 minutes

with maximum
brake.

with no brake; door
locks for
45 minutes

Call Beckman Coulter Field Service.

Wait 45 minutes then press CE
message; restart run. If problem persists, call
Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

Call Beckman Coulter Field Service.

to clear

GX-IM-5AB

4-3

Troubleshooting

Other Possible Problems

Table 4.1 Diagnostic Message Chart (Continued)

Diagnostic Number/

Message

Problem Result Recommended Action

I1–

Imbalance, see
manual

Rotor load is severely

out of balance

Rotor tie-down is not

properly fastened

Centrifuge is

misaligned (tilted)

Centrifuge was moved

during operation

Run shuts down
with maximum
brake

Run shuts down
with maximum
brake

Run shuts down
with maximum
brake

Run shuts down
with maximum
brake

• Make sure that tubes or bottles are

balanced and loaded symmetrically in the
rotor as described in the applicable rotor
manual.

• With swinging bucket rotors, remove the

buckets and clean and lubricate the pivot
pin sockets where the buckets contact as
described in the applicable rotor manual.
Unlubricated pivot pin sockets can
prevent the buckets from reaching
horizontal position, which can cause
imbalance.

• Press CE

to clear message.

Make sure rotor lid is properly aligned and tie­down T-bar is securely fastened. Press CE

to

clear message.

Level the centrifuge on the bench or table.
Press CE to clear message.

After the rotor comes to a complete stop,
open and close the centrifuge door, then
restart. Press CE

to clear message.

Drive error

(mechanical damage)

I2–

Imbalance error, see

System imbalance

error
manual

Other Possible Problems

Operating problems that may not be indicated by diagnostic messages are described in Table 4.2,
along with probable causes, listed in the probable order of occurrence, and corrective actions.
Perform the recommended corrective action in sequence, as listed. If you are unable to correct the
problem, call Beckman Coulter Field Service.

Run shuts down
with maximum
brake

Run shuts down
with maximum
brake

Call Beckman Coulter Field Service.

Press CE

to clear message, then restart run. If

problem persists, call Beckman Coulter Field
Service.

4-4

GX-IM-5AB

Table 4.2 Troubleshooting Chart

Problem Problem/Result Recommended Action

Troubleshooting

Retrieving Your Sample In Case of Power Failure

4

Rotor cannot achieve set
speed

Door will not open Rotor spinning Wait until the rotor stops.

Displays are blank Power not on Plug in the power cord; turn power on.

Chamber does not reach
selected temperature

Line voltage below rating Have a qualified service person measure line

voltage while the instrument is operating.

Electrical failure Make sure the power cord is securely

connected; call Beckman Coulter Field
Service.

Motor failure Call Beckman Coulter Field Service.

Power not on Plug in the power cord; turn power on.

Source power failure See Retrieving Your Sample In Case of Power

Failure, below.

Electrical failure Make sure the power cord is securely

connected; call Beckman Coulter Field
Service.

Centrifuge cannot maintain
selected temperature for
rotor in use at speed
selected

• Precool or prewarm rotors before running

at low or high temperatures.

• Precool rotor chamber by running a

30-minute cycle at the required
temperature with the speed set at
2000 rpm (Allegra X-12R).

• Make sure the air intake vent is clear.

• If using multiwell-plates for runs

exceeding 2 hours in a warm and/or
humid environment, speed reduction may
be required to maintain low sample
temperature.

Chamber icing
(Allegra X-12R)

Retrieving Your Sample In Case of Power Failure

If facility power fails you will have to restart the run when the power is restored. In the event of an
extended power failure, it may be necessary to trip the door-locking mechanism manually to
remove the rotor and retrieve your sample.

GX-IM-5AB

Excess humidity in chamber Wipe out moisture before each run.

Condensation build-up
between runs

• Leave door open between runs.

• Set temperature to a setting higher than

ambient temperature.

• Turn centrifuge power off.

4-5

Troubleshooting

1

Retrieving Your Sample In Case of Power Failure

1

Turn the power off and disconnect the power cord from the main power source.

• No displays or LEDs are lit.

2

Make sure that the rotor is not spinning.

• No sound or vibration comes from the centrifuge.

WARNING

Never attempt to override the door interlock system while the rotor is spinning.

3

Locate the release device cap underneath the bottom center of the centrifuge (not visible from
the centrifuge front). See Figure 4-1.

Figure 4.1 Door Latch Override

1. Release Device Cap

4

Use a flat-blade screwdriver to pry the cap loose.

5

Insert the screwdriver through the two cords and pull to release.

• The latch releases and the door opens.

a. If the rotor is still spinning, close the door and wait until it stops before attempting to remove it.

WARNING

Never attempt to slow or stop the rotor by hand.

6

Reinstall the cap in the opening after sample retrieval.

4-6

GX-IM-5AB

Introduction

This section contains care and maintenance procedures that should be performed regularly. For
maintenance not covered in this manual, contact Beckman Coulter Field Service (1-800-742-2345 in the
United States; worldwide offices are listed on the inside back cover of this manual) for assistance. User
messages and recommended actions are discussed in CHAPTER 4, Troubleshooting.

CHAPTER 5

Care and Maintenance

NOTE

It is your responsibility to decontaminate the centrifuge, as well as any rotors and/or accessories,

before requesting service by Beckman Coulter Field Service.

Instrument Care

WARNING

Any maintenance procedure requiring removal of a panel exposes the operator to
the possibility of electrical shock and/or mechanical injury. Therefore, turn the
power off and disconnect the instrument from the main power source, and refer
such maintenance to service personnel.

Maintenance

Perform the following procedures regularly to ensure continued performance and long service life
of the centrifuge.

1

Inspect the centrifuge chamber for accumulations of sample, dust, or glass particles from
broken sample tubes.

a. Clean as required (see Cleaning, below).

2

Check the air intake and exhaust for obstructions.

a. Keep vents clear and clean.

GX-IM-5AB

5-1

Care and Maintenance

Instrument Care

3

Wipe condensation out of the rotor chamber between runs with a sponge or clean cloth to
prevent chamber icing.

4

If chamber icing occurs, defrost the system and wipe moisture out of the chamber before use.

a. To defrost the system (Allegra X-12R), set the temperature to 30°C for 20 minutes.

conditions.)

• (These are suggested settings which may be adjusted as appropriate for your
laboratory

Cleaning

NOTE

Clean the centrifuge frequently. Always clean up spills when they occur to prevent corrosives or
contaminants from drying on component surfaces.

1

2

Before using any cleaning or decontamination methods except those recommended by the
manufacturer, users should check with the manufacturer that the proposed method will not damage the
equipment.

To prevent accumulations of sample, dust, and/or glass particles from broken sample tubes,
keep the chamber clean and dry by frequent wiping with a cloth or paper towel.

a. For thorough cleaning, wash the chamber using a mild detergent such as

Beckman Solution 555™, diluted 10 to 1 with water.

b. Rinse thoroughly and dry completely.

Clean the centrifuge exterior surfaces by wiping with a cloth dampened with Solution 555,
diluted 10 to 1 with water.

a. Do not use acetone.

3

Remove the rotor from the centrifuge and clean the drive hub regularly using Solution 555
(diluted 10 to 1 with water) and a soft brush.

a. Rinse thoroughly and dry completely.

Tube Breakage

If a glass tube breaks, and all the glass is not contained in the bucket or rotor, be sure to thoroughly
clean the chamber.

5-2

GX-IM-5AB

WARNING

Be careful when examining or cleaning the chamber and chamber gasket, as sharp
glass fragments may be embedded in their surfaces.

1

Examine the chamber gasket to make sure that no glass particles are retained in it.

a. Carefully remove any glass particles that may remain.

2

Carefully wipe away any glass particles that remain in the chamber.

Decontamination

Care and Maintenance

Circuit Breaker and Fuses

5

If the centrifuge and/or accessories are contaminated with radioactive or pathogenic solutions,
perform all appropriate safety and decontamination procedures. Refer to Chemical Resistances
(publication IN-175) to be sure the decontamination method will not damage any part of the
instrument

Sterilization and Disinfection

The centrifuge is finished with acrylic baking enamel. Ethanol (70%)* may be used on this surface.
See Chemical Resistances for chemical compatibilities of centrifuge and accessory materials.

While Beckman Coulter has tested ethanol (70%) and found that it does not damage the centrifuge,
no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection
is a concern, consult your laboratory safety officer regarding proper methods to use.

Circuit Breaker and Fuses

There are no user-replaceable fuses in the centrifuge.

If the centrifuge circuit breaker trips for any reason, the power switch will move to the off (
position. Reset the circuit breaker by turning the power switch back to the on (I) position. If it trips
again immediately, do not reset it. Call Beckman Coulter Field Service.

O)

* Flammability hazard. Do not use in or near operating centrifuges.

GX-IM-5AB

5-3

Care and Maintenance

Storage and Transport

CAUTION

Repeated attempts to reset the centrifuge circuit breaker can cause substantial
damage to electrical and electronic components.

Storage and Transport

Storage

To ensure that the centrifuge does not get damaged, contact Beckman Coulter Field Service for
specific instructions and/or assistance in preparing the equipment for transport or long-term
storage. Temperature and humidity conditions for storage should meet the environmental
requirements described under Specifications

in CHAPTER 1.

Supply List

Call Beckman Coulter Sales (1-800-742-2345 in the United States; worldwide offices are listed on the
back cover of this manual) for detailed information on ordering parts and supplies. For your
convenience, a partial list is given below.

NOTE

Replacement

Anti-rotation kit 392482

Supplies

Silicone vacuum grease (1 oz) 335148

Beckman Solution 555 (1 qt) 339555

Program library record 233679

Publications referenced in this manual can be obtained by calling Beckman Coulter at 1-800-742-2345
in the United States, or by contacting your local Beckman Coulter office.

5-4

GX-IM-5AB

Beckman Coulter, Inc.

Allegra X-12 Series Centrifuge Warranty

Subject to the exceptions and upon the conditions specified below and the warranty clause of the
Beckman Coulter, Inc. terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct
either by repair or, at its election, by replacement, any defects of material or workmanship which develop
within one (1) year after delivery of an Allegra X-12 Series centrifuge (the product), to the original buyer by
Beckman Coulter or by an authorized representative, provided that investigation and factory inspection by
Beckman Coulter discloses that such defect developed under normal and proper use.

Some components and accessories by their nature are not intended to and will not function for as long as one
(1) year. A complete list of such components or accessories is maintained at the factory and at each
Beckman Coulter District Sales Office. The lists applicable to the products sold hereunder shall be deemed to
be part of this warranty. If any such component or accessory fails to give reasonable service for a reasonable
period of time, Beckman Coulter will repair or, at its election, replace such component or accessory. What
constitutes either reasonable service and a reasonable period of time shall be determined solely by
Beckman Coulter.

Replacement

Any product claimed to be defective must, if requested by Beckman Coulter, be returned to the factory,
transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless
the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.

Conditions

Beckman Coulter makes no warranty concerning products or accessories not manufactured by it. In the event
of failure of any such product or accessory, Beckman Coulter will give reasonable assistance to the Buyer in
obtaining from the respective manufacturer whatever adjustment is reasonable in light of the manufacturer’s
own warranty.

Beckman Coulter shall be released from all obligations under all warranties, either expressed or implied, if
the product(s) covered hereby are repaired or modified by persons other than its own authorized service
personnel, unless such repair in the sole opinion of Beckman Coulter is minor, or unless such modification is
merely the installation of a new Beckman Coulter plug-in component for such product(s).

Disclaimer

IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF
FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER, INC., SHALL
HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING
OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR REPLACEMENT OF THE
PRODUCT.

GX-IM-5AB

Warranty-1

Beckman Coulter, Inc. Allegra X-12 Series Centrifuge Warranty

Warranty-2

GX-IM-5AB

Symbol
Symbol
Symbole
Símbolo

Title / Titel / Titre / Titulo / Titolo /

Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione

Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate

On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)

Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)

Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra

Earth (ground)
Erde
Terre
Tierra
Scarica a terra

!

/

Simbolo

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