Beckman Coulter Allegra 64R Instructions For Use Manual

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Instructions For Use

Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821

Allegra 64R

Compact Centrifuge

I M

E M

A C

RCF

T O

OPEN

L A

DOOR

T IM

T E

ENTER

E C

P U

F A

S T

S TO

STOP

S TA

GS30-IM-11AA September 2011

Allegra 64R

Beckman Coulter do Brasil Com e Imp de Prod de Lab Ltda Estr dos Romeiros, 220 - Galpao G3 - Km 38.5 06501-001 - Sao Paulo - SP - Brasil CNPJ: 42.160.812/0001-44

製造販売元 : ベックマン･コールター株式会社東京都江東区有明三丁目 5 番 7 号

TOC 有明ウエストタワー

贝克曼库尔特有限公司，美国加利福尼亚州，Brea 市，S. Kraemer 大街 250 号，邮编：92821 电话：(001) 714-993-5321

Compact Centrifuge

GS30-IM-11AA (September 2011)

All rights reserved. No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without prior written permission from Beckman Coulter, Inc.

Find us on the World Wide Web at:

www.beckmancoulter.com

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.

Alerts for Danger, Warning, Caution, and Note

DANGER

DANGER indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

Safety Notice

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

NOTE

NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

Safety During Installation and/or Maintenance

This centrifuge weighs 102.1 kg/225 lb. DO NOT attempt to lift or move it without assistance from another person.

Be sure to use the anchoring system to secure the centrifuge in place. The anchoring system is designed to reduce the possibility of injury or damage which could result from instrument movement in the event of a major rotor mishap.

Any servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury. Make sure that the power switch is turned off and the instrument is disconnected from the main power source, and refer such servicing to qualified personnel.

Do not replace any centrifuge components with parts not specified for use on this instrument.

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iii

Safety Notice

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect this equipment to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the instrument.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet

receptacle strip.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into the instrument and damage electrical or mechanical components.

Safety Against Risk of Fire

Certain electrical circuits within this equipment are protected by fuses against overcurrent conditions. For continued protection against the risk of fire, replace only with the same type and rating specified.

This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials (e.g., chloroform, ethyl alcohol, etc.) in this instrument nor handle or store them within the 30-cm (1-ft) clearance “safety envelope” surrounding the centrifuge.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the Beckman rotors and accessories designed for use in this centrifuge.

• Before starting the centrifuge, make sure that the rotor tie-down screw is securely fastened.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop the rotor by hand.

• Do not lift or move the centrifuge while the rotor is turning.

• If a glass tube breaks inside the chamber bowl, be careful when examining or cleaning the

gasket or chamber, as sharp glass fragments may be embedded in their surfaces.

• NEVER attempt to override the door interlock system while the rotor is spinning.

• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge (secured with antirotation

kit) while it is running. During operation you should come within the envelope only to adjust instrument controls, if necessary. Never bring any flammable substances within the 30-cm (1-ft) area surrounding the centrifuge. Never lean on the centrifuge or place items on the centrifuge while it is operating.

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Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Such materials should not be used in this instrument, however, unless all necessary safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to their use.

• Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi —further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health and safety guidelines.

Safety Notice

Chemical and Biological Safety

It is your responsibility to decontaminate the instrument and accessories before requesting service by our Field Service representative.

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Safety Notice

Chemical and Biological Safety

GS30-IM-11AA

Safety Notice,iii

Alerts for Danger, Warning, Caution, and Note,iii

Safety During Installation and/or Maintenance,iii

Electrical Safety,iv

Safety Against Risk of Fire,iv

Mechanical Safety,iv

Chemical and Biological Safety,v

Contents

Introduction,xiii

Certification,xiii

Scope of this Manual,xiii

Typographic Conventions,xiv

CFC-Free Centrifugation,xiv

Radio Interference,xiv

Recycling Label,xv

Specifications,xvi

Available Rotors,xviii

CHAPTER 1: Description,1-1

Introduction,1-1

Instrument Function and Safety Features,1-1

Instrument Function,1-1 Safety Features,1-2

Name Rating Plate,1-2

Chassis,1-3

Housing,1-3 Door,1-3 Rotor Chamber,1-3 Drive,1-4

vii

Contents

Temperature Sensing and Control,1-4

Controls and Indicators,1-4

Power Switch,1-4 Control Panel,1-5

CHAPTER 2: Installation Requirements,2-1

Introduction,2-1

Preinstallation Requirements,2-1

Space Requirements,2-2

Electrical Requirements,2-3

Test Run,2-4

CHAPTER 3: Operation,3-1

Introduction,3-1

Run Procedure,3-1

Preparation and Loading,3-2 Entering Run Parameters,3-4 Starting a Run,3-8 Changing Parameters During a Run,3-9 Stopping a Run,3-9

Unloading,3-10

Summary of Run Procedures,3-10

CHAPTER 4: Troubleshooting,4-1

Introduction,4-1

User Messages,4-1

category

Pollution degree

a. Temperature range depends on rotor in use and speed (see applicable rotor manual).

b. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation

must be expected.

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xvii

Introduction

Available Rotors

Rotor Profile Description

F1202 Fixed Angle 45° Angle

F2402H Fixed Angle 45° Angle

F3602 Fixed Angle 45° Angle

F0630 Fixed Angle 30° Angle

F0650 Fixed Angle 25° Angle

Max

RPM

Max

RCF (g)

Max RPM

at 4°C

Max RCF* ( g)

at 4°C

Max

Capacity

(mL)

Rotor Part

Number/

Rotor Manual

Number

30,000 64,400 28,500 58,120 12  2.0/1.5 364630

GS30-TB-006

26,000

61,970 21,000 40,430 24  2.0/1.5 361171

GS-TB-021

22,000 47,620/

41,670

26,200

59,860 21,500 40,310 6  38.5 361231

19,500 37,410/

32,700

36  2.0/1.5 364600

GS-TB-006

GS-TB-014

21,000 41,420 18,500 32,140 6  50 364610

GS30-TB-004

F0850 Fixed Angle 25° Angle

F0485 Fixed Angle 30° Angle

F0685 Fixed Angle 25° Angle

F1010 Fixed Angle 35° Angle

C0650 (Conical) Fixed Angle 25° Angle

C1015 (Conical) Fixed Angle 25° Angle

16,500 29,220 16,500 29,220 8  50 364640

GS-TB-003

20,000 40,700 17,500 31,160 4  85 364620

GS30-TB-007

15,500 26,320 15,000 24,650 6  85 364650

GS-TB-008

26,000

57,440 22,500 43,020 10  10 361221

GS-TB-007

10,000 10,400 10,000 10,400 6  50 364670

GS-TB-009

10,000 10,400 10,000 10,400 10  15 364680

GS-TB-011

xviii

GS30-IM-11AA

Introduction

Available Rotors

Max RCF* ( g)

at 4°C

Rotor Profile Description

S0410

Max

RPM

RCF (g)

Max RPM

at 4°C

10,000 10,730 10,000 10 730 4  10 364660 Swinging Bucket Four buckets

H6002

12,200 12,400 12,200 12 400 60  1.5/1.8 BioSafe Bowl Rotor

a. Values have been rounded off.

b. Measured at ambient temperature of 21°C.

c. Reduce maximum speed to maintain temperature control for runs exceeding 10 minutes.

Capacity

72  600 L 84  250 L

Max

(mL)

Rotor Part

Number/

Rotor Manual

Number

GS30-TB-005

363000

GS30-TB-002

GS30-IM-11AA

xix

Introduction

Available Rotors

GS30-IM-11AA

Introduction

This section provides a brief physical and functional description of the Allegra 64R centrifuge. The operating controls and indicators are also described; instructions for their use are in CHAPTER 3. Chemical compatibilities of materials listed in this manual can be found in Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.

Instrument Function and Safety Features

CHAPTER 1

Description

Instrument Function

The Allegra 64R is a refrigerated compact centrifuge that generates centrifugal forces required for a wide variety of applications. Together with any of several Beckman Coulter rotors designed specifically for use in these centrifuges, the applications include:

• Routine processing such as sample preparations, pelleting, extractions, purifications, concentrations, phase separations, receptor binding, and column centrifugations.

• Rapid sedimentation of protein precipitates, large particles, and cell debris.

• Preparation of subcellular organelles such as mitochondria, granules, and crude microsomes.

• Binding studies and separation of whole blood.

• Cell isolation.

• Crude protein preparations.

• Virus isolation.

• Nucleic acid plasmids and bacteriophages isolation.

The Allegra 64R centrifuge (Figure 1.1) is microprocessor-controlled, providing interactive operation. The instrument design features a brushless asynchronous motor, automatic rotor identification system, program memory that can repeat the previously used run parameters for a rotor, temperature control system, and a choice of acceleration and deceleration rates. User messages alert the operator to conditions that may need attention.

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1-1

Description

IMBALANCE

DECEL

RCF

F A

E N

D O

Name Rating Plate

Figure 1.1 The Allegra 64R Centrifuge

Safety Features

Allegra 64R centrifuges have been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft).

Instrument safety features include:

• The door has dual electromechanical door-locking mechanisms to prevent operator contact with spinning rotors. When the door is closed it locks automatically. It can be unlocked only by pressing the independent monitoring systems prevent the door from opening if the rotor is spinning.

• A steel barrier surrounds the rotor chamber to provide full operator protection.

• An overspeed system continuously monitors the rotor during centrifugation. The system

includes a magnetic sensor on the drive motor and magnets imbedded in the rotors. Throughout the run, checks are made to ensure that the rotor does not exceed set speed.

• An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor loads are severely out of balance. At low speeds, an incorrectly loaded rotor can cause imbalance. Rotor instability can also occur if the centrifuge is moved while running, or if it is not resting on a level surface.

For operator protection, an anchoring system is provided to secure the centrifuge in place. The anchoring system is designed to reduce the possibility of injury or damage that could result from centrifuge movement in the event of a major disruption.

OPEN DOOR key, and opened only when the power is on and the rotor is at rest. Two

Name Rating Plate

1-2

The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge. Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge.

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Chassis

Housing

Door

Description

Chassis

The centrifuge housing is made of sheet steel, finished with urethane paint. The control panel is covered by a protective overlay made of coated polycarbonate.

The solid-sheet aluminum door is secured to the housing by solid shafts. A dual electromechanical door lock system prevents operator contact with spinning rotors and prevents run initiation unless the door is shut and latched. The door is locked when a run is in progress and can be opened only when the rotor is stopped. (A light-emitting diode [LED] on the

OPEN DOOR key lights up when the

door can be opened.) In the event of a power failure, the door lock can be manually tripped for sample recovery (see CHAPTER 4, Troubleshooting).

Rotor Chamber

The rotor chamber is shown in Figure 1.2. The drive shaft, mounting plate, rubber boot surrounding the drive shaft, thermistor, and rotor detector are visible in the chamber bottom. A gasket system around the chamber opening assures sealing. (Instrument gaskets have not been designed as bioseals for aerosol containment.)

Figure 1.2 Interior View of the Rotor Chamber

1. Thermistor

2. Mounting Plate

3. Rotor Detector

GS30-IM-11AA

4. Boot

5. Drive Shaft

1-3

Description

Controls and Indicators

Drive

The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation. A tiedown screw is used to attach the rotor to the drive shaft. The resilient suspension ensures that loads will not be disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs during centrifugation. Maximum braking may be selected to reduce deceleration time, allowing fast processing of samples; alternately, delicate gradients may be preserved using slower deceleration.

Temperature Sensing and Control

With the power on, the temperature control system is activated when the door is closed. The run temperature can be set between –20 and +40°C. If no set temperature is entered, the centrifuge automatically selects the last entered temperature. (For the first run of a new centrifuge, the instrument selects 20°C as its operating temperature.) A thermistor in the rotor chamber continuously monitors chamber temperature. The microprocessor calculates the required chamber temperature to maintain the selected rotor temperature.

NOTE

In the unlikely event of a complete cooling system failure, the drive will switch off if the chamber

temperature reaches 65°C. Restarting the centrifuge will not be possible until the chamber is cooled.

Controls and Indicators

Power Switch

The power switch is located on the centrifuge side panel (see Figure 1.3). This two-position rocker switch (

NOTE The power must be turned on before the chamber door can be opened.

Figure 1.3 The Power Switch

I, on; O, off) controls electrical power to the centrifuge.

1-4

GS30-IM-11AA

Control Panel

SPEED TIME ACC/DEC

IMBALANCE

ENTER

PULSE

ACCEL

DECEL

TEMPRPM RCF ROTOR TIME

STOP START

FAST STOP

OPEN DOOR

The control panel (Figure 1.4), mounted at an angle on the centrifuge front for easy visibility and access, comprises touch keys—system keys and parameter keys—and digital displays. The panel also contains an

Figure 1.4 The Control Panel

Controls and Indicators

IMBALANCE light that flashes if rotor loads are severely out of balance.

Description

System Keys

The centrifuge operation is controlled through the system keys. Each key (except the PULSE key) has an LED in the upper left corner that lights to indicate that the key can be activated.

SPEED TIME TEMP °C ACC/DEC

ACCEL

DECEL

STOP START

IMBALANCE

OPEN DOOR

TEMPRPM RCF ROTOR TIME

FAST STOP

START Pressing the START key causes the centrifuge run to begin. This key can also be used to

abort a deceleration process and restart the centrifuge.

STOP The STOP key can be pressed to end a run. The centrifuge decelerates to a complete

stop according to the preselected deceleration curve. Deceleration can be terminated and the centrifuge restarted by pressing START again.

ENTER

PULSE

FAST STOP Pressing the FAS T STOP key causes the centrifuge to decelerate to a complete stop at

GS30-IM-11AA

the maximum rate. The deceleration cannot be interrupted; the centrifuge can only be restarted after the rotor stops and the door is opened and closed.

1-5

Description

SPEED TIME TEMP °C ACC/DEC

IMBALANCE

ENTER

PULSE

ACCEL

DECEL

TEMPRPM RCF ROTOR TIME

STOP START

FAST STOP

OPEN DOOR

Controls and Indicators

OPEN DOOR Pressing the OPEN DOOR key unlatches the centrifuge door locks and allows the door to

PULSE Pressing the PULSE key causes the installed rotor to accelerate up to the set speed for

Parameter Keys

The parameter keys are used to set run conditions. Except for the cursor and ENTER keys, parameter keys are located beneath the applicable digital displays, which show the parameters as they are input. Each key (except for the cursor keys) has an LED in the upper left corner that lights to indicate operational readiness. The LEDs also blink if an incorrect parameter is entered.

be opened. The centrifuge will accept this command only when the rotor is completely stopped and the OPEN DOOR key LED is lit.

short-duration runs (as long as the key is pressed). Deceleration, at the maximum rate, begins when the key is released.

 

(cursor keys)

The cursor keys are up and down arrow keys ( and ), which can be pressed to increment values up or down when setting parameters.

ENTER Parameter (speed, time, temperature, and acceleration or deceleration curve) changes

made while a run is in progress must be verified by pressing the ENTER key.

RPM When the RPM key is pressed the last digit in the SPEED display (0) flashes, indicating

that the speed can be entered in increments of 100 revolutions per minute (RPM). After the run starts, the actual RPM of the rotor is displayed.

RCF The RCF key can be used to select the speed setting by desired relative centrifugal field

(RCF). The corresponding RPM is automatically calculated and displayed during the run. If the RCF key is pressed during the run, the RCF value is shown in the SPEED display.

ROTOR The centrifuge memory contains a list of the rotors that can be used, together with

default parameters for each rotor. When the ROTOR key is pressed the number of the rotor used in the previous run is shown on the SPEED display. The rotor list can be scrolled through, using the cursor keys, until the required rotor number appears.

1-6

GS30-IM-11AA

Description

Controls and Indicators

TIME The TIME key is used to select the run duration. When the TIME key is pressed, the last

digit on the TIME display flashes, indicating that the time can be entered with the cursor keys.

• Timed run — Run time up to 9 hours and 59 minutes can be set. If the minutes

parameter exceeds 59, it is automatically converted into hours.

• Continuous run — If a run time of less than 0 or more than 9 hours and 59 minutes

is selected, continuous operation is activated. Time is not counted down, and the run will continue until the STOP or FAST STOP key is pressed.

TEMP The TEMP key is used to select run temperature. When the TEMP key is pressed, the

TEMP°C display flashes, indicating that the temperature can be entered with the

cursor keys. Temperature can be set between –20 and +40°C.

ACCEL The ACCEL key is used to select acceleration rates that will protect delicate gradients.

When the ACCEL key is pressed, the ACC/DEC display flashes, indicating that one of ten preset rates can be entered with the cursor keys. Acceleration rates are described in Ta b l e 3 . 1 (in CHAPTER 3).

DECEL The DECEL key is used to select deceleration rates that will maintain optimum

separation while protecting delicate gradients. When the DECEL key is pressed, the ACC/DEC display flashes, indicating that one of ten preset rates can be entered with the cursor keys. Deceleration rate selections are described in Ta b l e 3 . 1 (in CHAPTER 3).

Digital Displays

SPEED TIME TEMP °C ACC/DEC

ACCEL

DECEL

STOP START

IMBALANCE

OPEN DOOR

TEMPRPM RCF ROTOR TIME

FAST STOP

Digital displays indicate rotor speed, run time, rotor chamber temperature, and numbers that represent selected acceleration and deceleration profiles. When the power is turned on, they show the operating parameters of the most recent run performed before the power was turned off. The displays serve a dual purpose.

• When the run parameters are being set (the input mode), the displays show the set values (those selected by the operator). When a run- parameter key (for example, pressed, the appropriate display flashes to indicate that data can be entered.

• The actual (real-time) operating conditions of the centrifuge are displayed during the run, after

START is pressed.

ENTER

PULSE

TIME or RPM) is

NOTE

GS30-IM-11AA

Error messages (see CHAPTER 4) also appear on the displays, when applicable.

1-7

Description

Controls and Indicators

SPEED • In input mode the SPEED display shows the value of the parameter being set,

TEMP°C During standby (that is, the centrifuge is turned on but not spinning) and operation, the

ACC/DEC The ACC/DEC display shows the acceleration rate that was selected for the run. The

depending on the programming key pressed (RPM, RCF, or ROTOR). For example, if the ROTOR programming key is pressed, a rotor number appears on the SPEED display.

• During centrifugation, the SPEED display shows the speed of the rotor in RPM. If the RCF key is pressed while the centrifuge is running, the RCF value is displayed.

TIME • During a timed run (between 1 minute and 9 hours, 59 minutes), the TIME display

begins counting down when the rotor starts to spin and continues the countdown until deceleration begins. The TIME display indicates the remaining run time in hours and minutes.

• During a continuous run (less than 0 or more than 9 hours, 59 minutes selected), countdown time is not displayed. Instead, the infinity () symbol, indicating continuous operation, lights up and the TIME display shows time elapsed since the run start. After 9 hours and 59 minutes the timer will reset to 0 and continue counting elapsed time.

TEMP°C display shows the actual temperature inside the rotor chamber (2°C at an ambient temperature of 20°C).

deceleration curve number can be displayed by pressing the DECEL key.

1-8

GS30-IM-11AA

Introduction

Do not attempt to install this centrifuge. Its purchase price includes installation by Beckman Coulter personnel. Installation by anyone other than an authorized Beckman Coulter representative invalidates any warranty covering the instrument.

CHAPTER 2

Installation Requirements

WARNING

This centrifuge weighs 102.1 kg/225 lb. DO NOT attempt to lift or move it without assistance from another person.

Preinstallation Requirements

Preinstallation requirements have been sent prior to shipment of the instrument. The following information is provided in case the centrifuge must be relocated.

WARNING

Do not place the centrifuge near areas containing flammable reagents or combustible fluids. Vapors from these materials could enter the centrifuge air system and be ignited by the motor.

WARNING

Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge (secured with the antirotation kit) while it is running. No persons should be within this clearance boundary while the centrifuge is operating. Do not handle or store hazardous materials within the 30-cm (1-ft) area surrounding the centrifuge.

GS30-IM-11AA

2-1

Installation Requirements

61.0

24.0

7.6

3.0

66.0

26.0

84.0

33.0

38.0

15.0

in.

Space Requirements

Select a location away from heat-producing laboratory equipment, with sufficient ventilation to allow for heat dissipation.

Position the centrifuge on a level surface, such as a sturdy table or laboratory bench that can support the weight of the centrifuge (102.1 kg /225 lb) and resist vibration.

In addition to space for the centrifuge itself, allow 7.6-cm (3-in.) clearances at the sides and back of the centrifuge to ensure sufficient air circulation. (See Figure 2.1.)

Additional clearance is required on the left side to allow access to the power switch. The centrifuge must have adequate air ventilation to ensure compliance to local requirements

for vapors produced during operation.

Figure 2.1 Centrifuge Dimensions

2-2

GS30-IM-11AA

Installation Requirements

Electrical Requirements

Use the anti-rotation kit to secure the centrifuge to the bench or table as described in publication GS30-TB-003.

The anti-rotation system is designed to reduce the possibility of injury or damage that could result from instrument movement in the event of a major rotor mishap.

NOTE

During transport between areas with varying temperatures, condensation may occur inside the

centrifuge. Allow sufficient drying time before running the centrifuge.

Electrical Requirements

200-V centrifuge 190–210 VAC, 12 A, 50 Hz

200-V centrifuge 190–210 VAC, 12 A, 60 Hz

208-V centrifuge 198–218 VAC, 12 A, 60 Hz

230-V centrifuge 220–240 VAC, 12 A, 50 Hz

To reduce the risk of electrical shock, this centrifuge uses a 1.8-m (6-ft) three-wire electrical cord (to be attached to the IEC 320/CEE-20 AC power connector at the rear of the instrument) and plug to connect the centrifuge to earth-ground.The plug for use in North America is the NEMA 6-15P; a NEMA 6-15R socket (see Figure 2.2) should be available. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name rating plate affixed to the centrifuge.

• Never use a three-to-two wire plug adapter.

Figure 2.2 North American Electrical Socket

11.9 mm

17.6 mm

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet receptacle strip.

• If there is any question about voltage, have a qualified service person measure it under load while the drive is operating.

To ensure safety the centrifuge should be wired to a remote emergency switch (preferably outside the room where the centrifuge is housed, or adjacent to the exit from that room), in order to disconnect the instrument from the main power source in case of a malfunction.

GS30-IM-11AA

2-3

Installation Requirements

Test Run

Test R u n

NOTE The centrifuge must be plugged in and the power switch turned to on position (I) before the door

can be opened.

We recommend that you make a test run to ensure that the centrifuge is in proper operating condition following shipment. See CHAPTER 3 for instructions on operating the centrifuge.

After completing the test run, return the pre-addressed warranty card included with this literature. This will validate the centrifuge warranty and ensure your receipt of further information regarding new accessories and/or modifications as they become available.

2-4

GS30-IM-11AA

Introduction

This section contains operating procedures for the centrifuge, using any of the Beckman Coulter rotors designed for use in this centrifuge. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation. To prevent condensation, keep the centrifuge door closed and the power turned off

O) when the centrifuge is not in use.

(

CHAPTER 3

Operation

WARNING

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Operator error or tube failure may generate aerosols. Do not run potentially hazardous materials in this centrifuge unless all appropriate safety precautions are taken. Always use the appropriate rotors and adapters.

WARNING

The centrifuge must not be used in the vicinity of flammable liquids or vapors, and such materials should not be run in the centrifuge. During operation you should come within the 7.6 cm (3-in.) clearance envelope only to adjust instrument controls, if necessary. Never bring any flammable substances within the 30-cm (1 ft) area surrounding the centrifuge. Do not lean on the centrifuge or place items on the centrifuge while it is operating.

Run Procedure

The following detailed operating procedures are summarized at the end of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps.

GS30-IM-11AA

3-1

Operation

Run Procedure

Preparation and Loading

For fast temperature equilibration, cool or warm the rotor to the required temperature before the run.

NOTE

For high-speed runs at temperatures of 20°C or higher, prime the refrigeration system by running the

instrument at 10°C for 5 to 10 minutes beforehand to prevent overheating.

Check the name rating plate for the correct voltage, then plug the power cord into the wall receptacle.

Press the power switch to on (I).

Press the OPEN DOOR key and lift the door up; it will remain in the open position.

Use the T-handle wrench to turn the rotor tie-down screw to the left (counterclockwise).

a. Remove the tie-down screw.

CAUTION

Do not drop the rotor onto the drive shaft. The shaft can be damaged if the rotor is forced sideways or dropped onto it. Install the rotor by centering it over the shaft and carefully lowering it straight down.

Install the rotor (see Figure 3.1) according to the instructions in the rotor manual.

a. Always run the rotor with a balanced load. b. (If you are using a swinging bucket rotor, fill all four positions on the yoke with buckets.)

Attach the tie-down screw to the drive shaft by turning it to the right (clockwise).

Use the T-handle wrench to tighten the tie-down screw on the shaft.

CAUTION

If the rotor is left in the centrifuge between runs, make sure the rotor is seated on the drive shaft and the tie-down screw is tight before each run. (It is best to remove the rotor from the centrifuge if a long period between runs is anticipated.)

If using a fixed-angle rotor with a lid, attach the lid.

a. Tighten the lid with the T-handle wrench.

3-2

GS30-IM-11AA

Figure 3.1 Installing a Rotor

Operation

Run Procedure

1. Swinging Bucket Rotor

2. Fixed Angle Rotor

3. Lower the rotor straight down onto the drive shaft

GS30-IM-11AA

4. tighten the tie-down screw to the right (clockwise)

onto the drive shaft

5. attach the lid and tighten with the T-handle wrench

6. Seat filled buckets onthe yoke pins

3-3

Operation

SPEED TIME TEMP °C ACC/DEC

IMBALANCE

ENTER

PULSE

ACCEL

DECEL

TEMP RPM RCF ROTOR TIME

STOP START

FAST STOP

OPEN DOOR

Run Procedure

Close the centrifuge door and push firmly down on both sides of the door front until you hear both latches engage.

Entering Run Parameters

When the power is applied for the initial use (no previous runs), default values will be displayed (see

Figure 3.2). The centrifuge has an internal memory that stores previously used run parameters (or

programs) for each rotor that has been centrifuged. After the initial use, the program (parameters) of the latest previous run will be displayed when power is applied.

Figure 3.2 Default Parameters

1. The centrifuge will accelerate to 2000 RPM

2. The run duration will be 2 minutes

3. The chamber will be cooled to 20°C

4. The acceleration curve is 8

NOTE A Program Library is provided at the back of this manual. You can use this chart to record the

parameters of a run for later duplication of the run conditions.

When run parameters for a rotor have been entered, as described below, they will be retained in the centrifuge memory and can be recalled by simply entering the rotor number. The recalled program can then be used for the current run or can be altered as required.

Selecting a Rotor Number

The rotor number is engraved on each rotor (and on the lid of fixed angle rotors). The centrifuge internal memory contains a list of all rotors that can be run in the centrifuge, together with acceptable run parameters for each rotor. If you enter an unauthorized rotor number then press the

START key, an error message will be displayed and the run will be aborted shortly after the rotor

starts spinning.

3-4

GS30-IM-11AA

Press the ROTOR key.

Operation

Run Procedure

• The number of the last rotor previously centrifuged appears on the

Press the  or  cursor key until the number of the rotor in use is displayed.

Press ENTER.

• Nominal parameter values (time, temperature, speed, acceleration and deceleration

curves) for the selected rotor will be displayed.

— You can use these parameters or set new values for the run.

SPEED display.

Setting Run Speed

Centrifuge speed can be set for up to the maximum rated speed of the selected rotor. Either revolutions per minute (RPM) or relative centrifugal field (RCF) can be used to select speed. During centrifugation, the

NOTE

Use only the RPM mode to set run speeds for the H6002 bowl rotor. In the bowl rotor, RCF varies according to the tube in use, as indicated in the rotor manual (publication GS30-TB-002). If you use the RCF mode to set run speed, the centrifuge will select an RPM level based on the r

resulting in a speed too low to achieve the required RCF in the shorter tubes. Also note that the control panel will display the RCF calculated for the r

represent the RCF for shorter tubes. Refer to the rotor manual for the RCF values of shorter tubes.

SPEED display indicates the actual run speed of the rotor.

of the 1.8-or 1.5-mL tube, which will not accurately

max

of the largest tube,

max

Setting RPM

Press the RPM key.

• The last digit on the

100-RPM increments) with the cursor keys.

Press the  or  cursor key until the required RPM is displayed.

• The corresponding RCF will be automatically calculated by the centrifuge, but the RPM

value will be displayed during the run. — (You can check the RCF during the run by pressing the

running.)

SPEED display (0) flashes, indicating that the RPM can be entered (in

RCF key while the centrifuge is

GS30-IM-11AA

3-5

Operation

Run Procedure

Setting RCF

Press the RCF key.

• The last digit on the

Press the  or  cursor key until the required RCF is displayed.

• The corresponding RPM will be automatically calculated and the centrifuge will run at the calculated speed.

• The RPM value will be displayed during the run. — (You can check the RCF during the run by pressing the

running.)

SPEED display (0) flashes, indicating that the RCF can be entered.

RCF key while the centrifuge is

Setting Run Time

Run time can be set for either a timed run or continuous operation.

• Timed run — Time can be set for up to 9 hours and 59 minutes (if the minutes parameter entered

exceeds 59, it is automatically converted into hours). During centrifugation, the begins counting down when the rotor starts to spin and continues the count-down until deceleration begins. The minutes. When the time display reaches zero, the run ends.

• Continuous run — If a run time of less than 0 or more than 9 hours and 59 minutes is selected,

continuous operation is activated. Time is not counted down during continuous operation; instead, the infinity () symbol, indicating continuous operation, lights up and time elapsed since the run start is displayed. The run will continue until the pressed.

TIME display shows the time remaining in the run, in hours and

STOP or FAST STOP key is

TIME display

Press the TIME key.

• The last digit on the cursor keys.

Press the  or  cursor key until required run duration is displayed.

3-6

TIME display flashes, indicating that the time can be entered with the

GS30-IM-11AA

Setting Run Temperature

Run temperature can be set between –20 and +40°C.

Operation

Run Procedure

NOTE

Temperatures may vary slightly between instruments. If sample temperature is crucial, test

temperature settings on your instrument using water samples.

Press the TEMP key.

• The

TEMP°C display flashes, indicating that the temperature can be entered with the

cursor keys.

Press the  or  cursor key until the required run temperature is displayed.

NOTE

For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. For low-temperature runs, precool the centrifuge by running a 30-minute cycle at the required temperature (with a precooled rotor installed) with the speed set at about 2000 RPM.

For runs at room temperature and above at maximum rated speed, use acceleration rate 1 (50-Hz centrifuge) to avoid temperature spike during acceleration.

Setting Acceleration Rate

The ACCEL key is used to select acceleration rates that will protect delicate gradients. When the

ACCEL key is pressed, the ACC/DEC display flashes, indicating that one of the 10 preset acceleration

rates can be entered with the cursor keys. The selected rate will depend on the type of run you are performing. For pelleting runs, where sample mixing is not a concern, maximum acceleration can be used. However, if delicate gradients are being run, a lower setting may be needed. Acceleration rates are listed in Table 3.1.

Table 3.1 Acceleration and Deceleration Rates (in seconds per 1000 RPM)

Curve

a. Times are approximate; actual times will vary depending on the size and weight of the rotor in

use, rotor load, run speed, and voltage fluctuation.

GS30-IM-11AA

Acceleration Rate

(seconds per 1000 RPM)

1.0

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

6.0

Deceleration Rate

(seconds per 1000 RPM)

1.0

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

no brake

3-7

Operation

Run Procedure

Press the ACCEL key.

• The

Press the  or  cursor key until the required number is displayed.

ACC/DEC display flashes, indicating that the selected rate number can be entered with

the cursor keys.

Setting Deceleration Rate

The DECEL key is used to select deceleration rates that will maintain optimum separation. When

DECEL key is pressed, the ACC/DEC display flashes, indicating that one of the ten preset

the deceleration rates can be entered with the cursor keys. The selected rate will depend on the type of run you are performing. For pelleting runs, where sample mixing is not a concern, maximum brake can be used. However, if delicate gradients are being run, a lower brake setting may be needed. Deceleration rates are listed in Table 3.1.

Press the DECEL key.

• The

ACC/DEC display flashes, indicating that the selected number can be entered with the

cursor keys.

Press the  or  cursor key until the required number is displayed.

Starting a Run

The run can be started using the parameters in memory from a previous run, or using new or changed parameters that you enter using the procedure described above.

Check that all parameters are correct and the door is shut and latched.

Press the ENTER key, then the START key.

• As the run begins, the instrument rotor identification system compares the rotor in the

• An incorrect rotor identification, or set speed greater than the rotor’s maximum permitted

chamber to the entered rotor number and the speed entered.

speed, will result in an error code and the centrifuge will shut down. — (See CHAPTER 4, Troubleshooting, for information on error codes.)

3-8

GS30-IM-11AA

Operation

Run Procedure

a. The error must be cleared and an appropriate speed entered before the centrifuge can be

started.

• Throughout the run, checks are made to ensure that the rotor does not exceed

set speed.

• The

• A blinking LED at the bottom of the

SPEED display indicates the rotor speed in RPM.

— (The RCF can be checked by pressing the

TIME display indicates that the run is in progress.

— This display also shows the time remaining in the run (or  and elapsed time for

continuous operation).

RCF key.)

WARNING

Do not attempt to override the door interlock system while the rotor is spinning.

CAUTION

Do not lift or move the centrifuge while the rotor is spinning.

NOTE

Power line interruptions automatically initiate an intended rotor drive deceleration and run time reset. Power interruptions are momentary power dropout events of partial or complete power cycles. The centrifuge panel display may momentarily blank and recover. The entered run time value and the rotor decelerates under the entered run deceleration profile. Actual rotor speed is displayed during deceleration. Allow the rotor to come to a complete stop. To restart the centrifuge, press ENTER, then START.

TIME display resets to the

Changing Parameters During a Run

While a run is in progress, run parameters (speed, time, temperature, and acceleration or deceleration rate) can be altered without stopping the run. Run duration can also be changed from continuous to a specified time period, or from a specified time period to continuous.

NOTE

Use the parameter keys as described under Entering Run Parameters. Parameter changes made during a run must be verified by pressing the centrifugation:

GS30-IM-11AA

The deceleration rate cannot be changed after deceleration starts.

Press the RPM key.

• The last digit on the with the cursor keys.

Press the  or  cursor key until the required RPM is displayed.

ENTER key. For example, to change run speed during

SPEED di spla y fla shes , ind icat ing t hat t he RP M can be ra ised or l ower ed

3-9

Operation

Unloading

Press the ENTER key.

• The current RPM value will be displayed, changing to the new value as the rotor accelerates

• The corresponding RCF will be automatically calculated by the centrifuge.

Stopping a Run

A timed run will end automatically when the TIME display counts down to zero. To end a run in progress for any reason:

Press the STOP key for normal deceleration as selected by the deceleration curve.

(or)

or decelerates to the new speed selected.

Unloading

After completing a run, unload the rotor following the instructions in the applicable rotor manual.

Press the

NOTE

After the rotor stops spinning and the OPEN DOOR light comes on, press the OPEN DOOR key to release the door latches, then open the door.

NOTE

FAST STOP key for deceleration at the maximum rate (see Table 3.1).

If you press FAST STOP the deceleration process cannot be interrupted; the rotor must come to a

complete stop and the door opened and closed before the centrifuge can be restarted.

To prevent chamber icing, use a sponge to wipe condensation out of the chamber bowl

between runs.

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

3-10

GS30-IM-11AA

Summary of Run Procedures

For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. For low-temperature runs, precool the centrifuge by running a 30-minute cycle (with a precooled rotor installed) at the required temperature with the speed set at 2000 RPM.

Press the POWER switch to on (I).

Operation

Summary of Run Procedures

a. Open the centrifuge door (press the

Install the rotor according to the instructions in the rotor manual.

a. Always run the rotor with a balanced load.

Close the centrifuge door and push firmly down on it until you hear both latches engage.

Enter run parameters:

a. Select a rotor number — b. Set run speed — RPM,  or ; or RCF,  or  c. Set run duration — d. Set run temperature — e. Select acceleration rate (0 through 9)— f. Select deceleration rate (0 through 9)—

Check that all parameters are correct and the door is shut and latched, then press the ENTER key, then the

START key.

ROTOR,  or , ENTER

TIME,  or 

TEMP,  or 

OPEN DOOR key and lift the door up).

ACCEL,  or  DECEL,  or 

WARNING

Never attempt to override the door interlock system while the rotor is spinning.

CAUTION

Do not lift or move the centrifuge while the rotor is spinning.

Wait for the set time to count down to zero, or end the run by pressing either the STOP key or

FAST STOP key.

the

After the rotor stops spinning and the OPEN DOOR light comes on, press the OPEN DOOR key to release the door latch; open the door.

GS30-IM-11AA

3-11

Operation

Summary of Run Procedures

Unload the rotor according to instructions in the appropriate rotor manual.

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

3-12

GS30-IM-11AA

Introduction

This section lists possible malfunctions, together with probable causes and corrective actions required. Maintenance procedures are contained in CHAPTER 5. For any problems not covered here contact Beckman Coulter Field Service.

CHAPTER 4

Troubleshooting

NOTE

It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before

requesting service by Beckman Coulter Field Service.

User Messages

CAUTION

If the message SEr appears on the display, do not press any keys while the message is displayed. Turn the centrifuge power off ( message. This message indicates that you have inadvertently accessed the service mode. Pressing any keys while in this mode could erase the centrifuge memory and critically interfere with future operation.

If a problem occurs during operation, the rotor will decelerate to a stop and an error code will appear on the malfunction. Refer to Table 4.1 to determine the nature of the problem and recommended actions. If you are unable to correct the problem, call Beckman Coulter Field Service. To help diagnose and correct the problem, provide as much information as you can:

• The error number that appears on the display.

• The operating situation when the error occurred (rotor in use, speed, load type, etc.).

O) and back on (I) to clear the

SPEED display. Messages may result from incorrect input or from an equipment

• Any unusual environmental and/or operating conditions (ambient temperature, voltage

fluctuations, etc.).

• Add any other information that may be helpful.

GS30-IM-11AA

4-1

Troubleshooting

User Messages

Table 4.1 Error Message Chart

Error Number

1 through 23,

25 and 26,

28 through 30

Problem Result Recommended Action

Microprocessor or mechanical malfunction

32, 36, 39 and 40,

44 through 60,

63 through 68

23 and 24, 27, 31,

33 through 35,

37 and 38,

Microprocessor or mechanical malfunction

41 through 43, 62

69 through 77 Microprocessor

malfunction

78 through 80 Error during closing of

door

81 “Door open”

detected during run

Deceleration to full stop; run cannot be

After rotor comes to a complete stop, turn the power off (O), then back on (I) to reset.

restarted

Deceleration to full stop; run cannot be

After rotor comes to a complete stop, open and close the centrifuge door, then restart.

restarted

Run cannot start until error is cleared

Turn the power off (O), then back on (I) to reset.

Run cannot start 1. Remove debris in latch.

2. Close lid quickly.

3. Turn the power off (O), then back on (I) to

reset.

Maximum deceleration to full stop

After rotor comes to a complete stop, shut the centrifuge door, turn the power off (O), then back on (I) to reset.

82, 83 Door does not open — See Emergency Access, below.

84 Heat sink

overtemperature

through

Rotor chamber overtemperature

Deceleration to full stop

1. Check that ambient temperature is within

a the limits shown in

2. Check air inlets and exhausts for

obstructions.

After the motor has cooled, restart. If the problem persists, call Beckman Coulter Field Service.

90 through 96 Temperature sensor

malfunction

Maximum deceleration to full

Turn the power off (O), then back on (I) to reset.

stop

98 Rotor not recognized Maximum

deceleration to full stop

Check that the installed rotor is usable in the centrifuge. If incorrect, install an authorized rotor; if rotor is authorized and problem persists, call Beckman Coulter Field Service.

99 Rotor recognized but

incorrect

Maximum deceleration to full

Input correct parameters for the rotor in use, then restart.

stop

a. If the recommended action does not correct the problem, call Beckman Coulter Field Service.

Specifications.

4-2

GS30-IM-11AA

Other Possible Problems

Imbalance LED lights and rotor decelerates to stop

Rotor cannot achieve set speed

Door will not open 1. Rotor spinning

Displays are blank 1. Power not on

TEMP °C display flashes

1. Rotor is out of balance

2. Centrifuge is misaligned

(tilted)

3. Centrifuge was moved

during operation

4. Drive error (mechanical

damage)

1. Line voltage below rating

2. Electrical failure

3. Motor failure

2. Power not on

3. Source power failure

4. Latch stuck

2. Electrical failure

3. Fuse blown

Chamber temperature is >25°C higher than selected temperature

1. Check to be sure the rotor is in good condition and is loaded

symmetrically around the center of rotation, with containers of equal weight and density (within 6 grams) opposite each other.

2. Align the centrifuge on the bench or table.

3. After the rotor comes to a complete stop, open and close

the centrifuge door, then restart.

4. Call Beckman Coulter Field Service

1. Have a qualified service person measure line voltage while

the instrument is operating.

2. Make sure both ends of the power cord are securely

connected; call Beckman Coulter Field Service.

3. Call Beckman Coulter Field Service

1. Wait until the rotor stops.

2. Plug in the power cord; turn power on (I).

3. See Emergency Access, below.

4. See Emergency Access, below.

1. Plug in the power cord; turn power on (I).

2. Make sure both ends of the power cord are securely

connected; call Beckman Coulter Field Service.

3. Call Beckman Coulter Field Service.

Precool rotors before running at low temperatures. Precool rotor chamber by running a 30-minute cycle at the desired temperature with the speed set at about 2000 RPM. If a lower temperature deviation alert is required, contact Beckman Coulter Field Service.

Chamber does not reach selected temperature

GS30-IM-11AA

Centrifuge cannot maintain selected temperature for rotor in use at speed selected

Refer to appropriate rotor bulletin for temperature and speed requirements. Also, precool rotors before running at low temperatures. Precool rotor chamber by running a 30-minute cycle at the desired temperature with the speed set at 2000 RPM.

4-3

Troubleshooting

Emergency Access

If the facility power fails only momentarily, the centrifuge will resume operation when power is restored and the rotor will return to set speed. However, if the rotor comes to a complete stop you will have to restart the run when the power is restored. In the event of an extended power failure, it may be necessary to trip the door-locking mechanism manually to remove the rotor and retrieve your sample (see Figure 4.1).

WARNING

Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and/or mechanical injury. Therefore, turn the power off and disconnect the instrument from the main power source, and refer such maintenance to qualified service personnel.

Figure 4.1 Emergency Door Release

1. Use an Allen wrench to loosen four screws

2. Pull out (away from bowl) on latch release

(behind guard plate)

Turn the power switch to off (O) and disconnect the power cord from the main power source.

3. Use an open-end wrench to remove four bolts

(not visible from front)

4. Pull out (away from bowl) on latch-release

WARNING

Never attempt to override the door interlock system while the rotor is spinning.

4-4

GS30-IM-11AA

Troubleshooting

Emergency Access

Make sure that the rotor is not spinning.

a. Do not proceed if there is any sound or vibration coming from the drive.

Use the 2.5-mm ball-head Allen wrench (964774) to loosen the four screws on the centrifuge front panel (see Figure 4.1).

Use a 7-mm open-end wrench to remove the bottom three bolts holding the front panel to the centrifuge.

Carefully lower the front panel to the bench top to expose the latch releases (see Figure 4.1).

Pull out (away from the bowl) on the latch releases until the latches “click” or the door pops open.

a. If the rotor is still spinning, close the door and wait until it stops before attempting to

remove it.

WARNING

Never try to slow or stop the rotor by hand.

b. After removing the rotor, replace the front panel by following the instructions above, in

reverse order.

GS30-IM-11AA

4-5

Troubleshooting

Emergency Access

4-6

GS30-IM-11AA

Introduction

For maintenance not covered in this manual, call Beckman Coulter Field Service. User messages are discussed in CHAPTER 4, Troubleshooting. Refer to the applicable rotor manual and to Chemical

Resistances (publication IN-175) for instructions on the care of rotors and their accessories.

CHAPTER 5

Care and Maintenance

NOTE

Do not use alcohol or other flammable substances in or near operating centrifuges.

It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before

requesting service by Beckman Coulter Field Service.

WARNING

Any maintenance procedure or servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury. Make sure that the power switch is off (O) and the centrifuge is disconnected from the main power source, and refer such servicing to qualified service personnel.

Maintenance

Preventive Maintenance

The following procedures should be performed regularly to ensure continued performance and long service life of the centrifuge.

Regularly inspect the interior of the rotor chamber for accumulations of sample, dust, or glass particles from broken sample tubes.

a. Clean as required (see Cleaning, below), as these accumulations can result in rotor

vibrations.

Regularly check the air intake and exhaust vents for obstructions.

a. Keep vents clear and clean.

GS30-IM-11AA

5-1

Care and Maintenance

Cleaning

Use a sponge to wipe condensation out of the chamber bowl between runs to prevent chamber icing.

If chamber icing occurs, defrost before use.

To prevent the rotor from sticking, lubricate the drive shaft with Spinkote at least once a month, and after each cleaning.

Before using any cleaning or decontamination methods except those recommended by the

NOTE

manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment.

Frequent cleaning is recommended to prolong the life of the centrifuge.

Always clean up spills when they occur to prevent corrosives or contaminants from drying on component surfaces.

To prevent accumulations of sample, dust, and/or glass particles from broken sample tubes, keep the interior of the rotor chamber clean and dry by frequent wiping with a cloth or paper

towel.

Clean the drive shaft, shaft cavity, threads, and the tie-down screw at least once a week using a mild detergent such as Beckman Solution 555™ and a soft brush.

a. Dilute the detergent 10 to 1 with water. b. Rinse thoroughly and dry completely. c. Lubricate the drive shaft with Spinkote after cleaning.

Wash the bowl using a mild detergent such as Solution 555. Rinse thoroughly and dry completely.

a. Rinse thoroughly and dry completely.

Clean the centrifuge case and door by wiping with a cloth dampened with Solution 555.

a. Do not use acetone or other solvents.

5-2

GS30-IM-11AA

Tube Breakage

If a glass tube breaks, and all the glass is not contained in the bucket or rotor, it will be necessary to thoroughly clean the interior of the chamber bowl.

WARNING

Be careful when examining or cleaning the sealing gasket or chamber, as sharp glass fragments may be embedded in their surfaces.

Examine the gasket to make sure that no glass particles are retained in it.

a. Carefully remove any glass particles that may remain.

Carefully wipe away any glass particles that remain in the bowl.

Care and Maintenance

Tube Breakage

Decontamination

If the instrument and/or accessories are contaminated with radioactive or pathogenic solutions, perform appropriate decontamination procedures. Refer to Chemical Resistances to be sure the decontamination method will not damage any part of the instrument.

Sterilization and Disinfection

The centrifuge is finished with urethane paint. Ethanol (70%)* may be used on this surface. See Chemical Resistances for more information regarding chemical resistance of centrifuge and accessory materials.

While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

* Flammability hazard. Do not use in or near operating centrifuges.

GS30-IM-11AA

5-3

Care and Maintenance

Storage and Transport

Storage

Before storing a centrifuge for an extended period, return it to its original shipping container to protect it from dust and dirt. Temperature and humidity conditions for storage should meet the environmental requirements described under Specifications.

Returning a Centrifuge

Before returning a centrifuge or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the RGA form and for packaging and shipping instructions.

To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity. Sterilization and decontamination must be done before returning the parts.

All parts must be accompanied by a signed note, plainly visible on the outside of the box, stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach

this notification will result in return or disposal of the items without review of the reported problem.

Supply List

NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1-800-742-2345

in the United States, or by contacting your local Beckman Coulter office.

Contact Beckman Coulter Sales (1-800-742-2345 in the United States) for information about ordering parts and supplies. For your convenience, a partial list is given below. See the Beckman Coulter Benchtop Rotors, Tubes & Accessories catalog (BR-9742, available at www.beckmancoulter.com) for detailed information on ordering rotors, tubes, and accessories. Refer to the rotor manual or materials and supplies needed for rotors.

Replacement Parts

Rotor tie-down screw 361367

T-handle wrench 361371

Anti-rotation kit 361377

Emergency latch-release tool (ball-head allen wrench) 964774

5-4

GS30-IM-11AA

Supplies

Care and Maintenance

NOTE For MSDS information, go to the Becman Coulter website at www.beckmancoulter.com.

Spinkote lubricant (2 oz) 306812

Silicone vacuum grease (1 oz) 335148

Beckman Solution 555 (1 qt) 339555

Supply List

GS30-IM-11AA

5-5

Care and Maintenance

Supply List

5-6

GS30-IM-11AA

Procedure

Program

Number

User Date Purpose

APPENDIX A

Program Library

Record the program parameters on the chart below as a quick reference when duplicating run conditions. Refer to CHAPTER 3 of this manual for the numbers representing acceleration and

deceleration (ACCEL/DECEL) rates.

Speed Time

RPM RCF

(hrs/ min)

Te mp

(°C)

ACCEL

Rate

DECEL

Rate

GS30-IM-11AA

A-1

Program Library

Procedure

Program

Number

User Date Purpose

Speed Time

RPM RCF

(hrs/

min)

Te mp

(°C)

ACCEL

Rate

DECEL

Rate

A-2

GS30-IM-11AA

Beckman Coulter, Inc.

Allegra 64R, GS-15 Series, Allegra 21 Series, and

Spinchron 15 Series Centrifuge Warranty

Subject to the exceptions and upon the conditions specified below and warranty clause of the Beckman Coulter, Inc. terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair, or, at its election, by replacement, any defects of material or workmanship which develop within one (1) year after delivery of an Allegra 64R, Allegra 21, GS-15, or Spinchron 15 series centrifuge (the product), to the original buyer by Beckman Coulter or by an authorized representative, provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use.

Some components and accessories by their nature are not intended to and will not function for as long as one (1) year. A complete list of such components or accessories is maintained at the factory and at each Beckman Coulter District Sales Office. The lists applicable to the products sold hereunder shall be deemed to be part of this warranty. If any such component or accessory fails to give reasonable service for a reasonable period of time, Beckman Coulter will repair or, at its election, replace such component or accessory. What constitutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter.

Replacement

Any product claimed to be defective must, if requested by Beckman Coulter, be returned to the factory, transportation charges prepaid, and will be returned to buyer with the transportation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.

Except as specifically noted above, Beckman Coulter makes no warranty or representation, either expressed or implied, with respect to this software or its documentation including quality, performance, merchantability, or fitness for a particular purpose.

Conditions

Beckman Coulter shall be released from all obligations under all warranties, either expressed or implied, if the product(s) covered hereby are repaired or modified by persons other than its own authorized service personnel, unless such repair in the sole opinion of Beckman Coulter is minor, or unless such modification is merely the installation of a new Beckman Coulter plug-in component for such product(s).

Disclaimer

IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT NEITHER BECKMAN COULTER, INC. NOR ITS SUPPLIERS SHALL HAVE ANY LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.

PN 7654321A

Warranty-1

Beckman Coulter, Inc. Allegra 64R, GS-15 Series, Allegra 21 Series, and Spinchron 15 Series Centrifuge Warranty

Warranty-2

PN 7654321A

Symbol Symbol Symbole Símbolo

Title / Titel / Titre / Titulo / Titolo /

Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione

Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate

On (power) Ein (Netzverbindung) Marche (mise sous tension) Encendido Acceso (sotto tensione)

Off (power) Aus (Netzverbindung) Arrêt (mise hors tension) Apagado Spento (fuori tensione)

Protective earth (ground) Schutzleiteranschluß Liaison à la terre Puesta a tierra de protección Collegamento di protezione a terra

Earth (ground) Erde Terre Tierra Scarica a terra

Simbolo

www.beckmancoulter.com

Beckman Coulter Allegra 64R Instructions For Use Manual

Specifications and Main Features

Frequently Asked Questions

User Manual