Bausch and Lomb Spectronic 70 User manual

THE

BAUSCH

&

LOMB

SPECTRONIC®

Before

“Initial

Preliminary

operating

1.

Check (Refer

dure).

2.

Install the

ware “Sample

3.

Turn

five-minute

Checkout

that to

that

power

OPERATING

the

instrument,

Procedures”

meter

reads

EXACTLY

‘’Meter

are

Compartments’’).

sample

most

ON

with

warmup,

Zero

Adjust’’

compartment

suitable

rear

minimum.

read

are

to

panel

and

become

complete

0%T

with

for

adjustment

and

select

the

analysis.

Power

70

SPECTROPHOTOMETER

PROCEDURES

familiar

before

power

the

(Refer

Switch,

first

operation

OFF.

proce-

glass-

to

allow

with

|

the

entire

of

Operator s Manual.

instrument.

Be

sure

that

Sample

1.

Set

Analytical

2.

Insert set

100%T

3.

Insert read

sample

*IMPORTANT

SITIVITY

THE

"MIRROR

MAINTENANCE

333041-10040

Measurement

Step

Wavelength Wavelength

reference

unknown

OR

blank,

(OA)*

sample,

value.*

-

IF

INSTRUMENT

EXCESSIVE

ALIGNMENT" SECTION.

HAS

NOISE,

PROCEDURE

Use

Drive

100%T/Zero A Knob

Meter

Display

REDUCED

FIRST

REFER

IN

SEN-

TO

THE

Knob

PRINTED

IN

U.S.A.

The

SPECTRONIC®

reading,

continuous

single-beam,

325-925nm

70

spectrophotometer

grating

wavelength

instrument

range,

is a direct-

which

provides

using

automatic

SECTION

1

DESCRIPTION

interchange

a

radiant The

Figure

energy

locations

1.

of

phototubes

and

second-order

of

principal

and

automatic

filters.

operating

insertion

features

are

of

shown

stray

in

Meter

Universal Test

Holder

Figure

Tube

Multiple

Compartment

1.

Location

Sample

of

Operating

Sample

Handle

Features

Selector

Wavelength

Display

100%T/Zero

Control

A

Power Switch

Wavelength

Selector

1.1

Specifications

Wavelength Spectral

Wavelength Stray Photometric

Photometric

Photometric Photometric Photometric

External

Power

Size

Weight

1.2 The

Slitwidth

Radiant

0%T

100%T

Reguirements

i

Meter

instrument

Range

Readability

Energy

Range

Accuracy

Readability Noise Stability

Drift

Output

displays

*

photometric

325-925nm

8.0nm

0.1nm

0.5%

at

340nm

0-100%T

0-2.0A

1.0%T

at

meter

0.5%T

at

analog

output

0.2%T

(1/5

division)

0.1%T

0.2%T/day

0.1%T/15

0.2%

0-1.0

100,

50,

48.2em W x

34.3ет

(ша. х Юм. х 13

15.9

T/hour

VDC

115,

60

Hz,

kg

minutes

220,

90

25.4cm H x

О

(35.0

lbs.)

values

240VAC;

Watts

on

1/2

an

in.)

8-in.

meter

with

an

anti-parallax transmittance additional to

2.0

1.3

The length wavelength

1.4

The

100%T

The Shifting only a reverse control knob

100%T/zero Reverse operation,

control

revolutions

coarse

logarithmic

absorbance

Wavelength

wavelength

within

is

shown

100%T/Zero

100%T/zero A control

or

0.0A

meter

control

in

adjust.

has a unique

from

coarse

is

best

utilized

the

required

A

the

rotation

allowing a very

remains

of

the

mirror.

using a linear

scale

for

units.

Selector

selector

the

A

rotation

setting

in

is

range

on

the

Control

is

readout

to

fine

of

in

the

direction

and

accurate

fine

adjust

knob, and

used

of

wavelength

used

coarse/fine

is

the

slightly

the

then

The

0-100%

absorbance

to

select

325-925nm.

to

set

on a blank

entirely

following

automatic,

knob.

For

to

achieve

overshoot

fine

adjust

setting

for

approximately 1 1/2

automatically

meter

scale,

and

readout

the

desired The

display.

the

instrument

reference

adjustment

this

manner:

the

will

to

be

displays

has

from

wave-

selected

for

sample.

feature.

requiring

reason,

Turn

desired

setting.

come

made.

reverts

%

an

0.0

a

the the

into The

to

1.5

Universal

The

universal

to

20mm

in

diameter

better

than

0.5%.

To

use

the

holder,

cuvette Insert vertical the used,

100mm-pathlength sample

thumbscrews on opening.

1.6

The following

a.

b. c.

rectangular path shifted with reference

with

the

cuvette

mark

instrument.

install a light

It

is

not

compartment.

To

remove

the

compartment

Multiple

front-loading

sampleholders:

Conventional short-path,

Conventional

Filter

plates

The

multiple

cells

at

into the

automatic

blank

sample

necessary

cuvettes

one

Test

Tube

test

tube

close

to

through

on

the

cuvette

Close

shield,

cylindrical

the

holder,

(shown

wall,

Sample

multiple

rectangular

long-path,

cylindrical

up

to

sample

time.

optical

reset

in

the

Holder

holder

accepts

and

positions

the

front-loading

the

horizontal

the

top-loading

aligned

the

top

door.

cat.

no.

33-30-22,

to

remove

cells

unfasten

in

Figure

and

2)

remove

Compartment

sample

cuvettes,

or

constant-temperature.

cells

up

3”

square.

compartment

or

two

conventional

The

cuvettes

beam

in a 1-2-3

to

position 1 so

sample

compartment

test-tube

them

with a precision

mark

to

the

indicator

If

overlength

over

the

holder

are

used

the

which

position

it

through

compartment

including

to

100mm

can

or

cells

sequential you

cuvettes

door

and

fill

on

the

cuvette.

door,

with

mark

cuvettes

the

cuvette.

except

in

the

multiple

two

mounting

the

accepts

flow-thru,

pathlength.

accept

cylindrical

can

be

quickly

fashion

can

keep

at

all

times.

up

of

the

on

are

when

holder

front

the

three

long-

the

The

compartment removable

The

attached

will pathlength

holder 100mm

tube mounting to

the

double

A

is

available

C

for Sequencing

shown

cuvette

removable

to

the

accept

up cylindrical

and

remove

pathlength

holder

must

thumbscrews

instrument.

spring

constant-temperature

to

samples

in

Figure

corresponding

handle cylindrical Pushing

position

cell opened, to

insert sample

cuvettes

into loading are

is

3.

in

the

position

If

the

analytical

it

compartment,

Proper

the

doors

taken.

pulled

cell)

in

on

Position 3 aligns

optical

sample

1.

into

analytical

or

cells

sample

is

provided

holder.

cuvette

sample

to

three

it

cells

also

The

clip

on

maintain

requiring

is

provided

1.

to

the

three

all

is

precisely

the

handle

path.

selector

procedure

the

spring

to

be

compartment.

must

be

with a front-loading

holder

compartment

10mm

cells

are

from

are

be

removed

(shown

cylindrical

the

sample

cuvette

three

temperature

by

The

cuvettes

the

way

Whenever

handle

clip

as

shown

procedure

filled

closed

(shown

cuvettes.

to

be

the

sample

to

be

in

Figure

cells

compartment.

holder

lem

square

the

sample

handle

out,

aligned

selects

the

front

the

is

requires

on

the

in

Figure

requires

with

samples

Both

before

witha

used,

by

in

position

automatically

any

in

Figure

spring

If

20mm- unclip

compartment.

the

universal

unscrewing

2)

which

are

inserted

(cat.

no.

cuvettes

control.

selector

has

three

the

holder.

the

rear

in

the

optical

2,

cuvette

the

or

front-loading

use

of a filter

left-hand

4.

all

conventional

before

the

front-

readings

door

and

a

3)

is

latch

and

or

50mm-

the

cuvette

If

test

the

two

fasten

it

into

the

33-30-14) at

5° - 55°

handle,

positions

When

the

cuvette

and

(or

path.

finally

cylindrical

door

is

returned

plate,

side

of

the

insertion

and

top-

of

any

kind

Figure

2.

Location Test

Tube

of

Mounting

Holder

Thumbscrews

on

Universal

Figure

3.

Removable

Compartment

Cuvette

Holder

Removable

Cuvette

Holder

in

Multiple

Sample

1.7

External

The

external

permits

including the

DR-37

The

six

from

the

The

photometric

a

DC

analog

nominal

2).

Output

output

attachment

the

Bausch & Lomb

digital

pins

rear

1.2VDC

readout

of

the

of

the

voltage. A 100%T

signal

Jack

jack

on

of a variety

10”

listed

jack

are

numbered

instrument.

6-4-2 5-3-1

transmittance

on

this

the

rear

of

external

Strip

in

Section

value

meter

(referenced

of

Chart

5.1,

as

is

present

display

the

instrument

readout

Recorder

Accessories.

follows,

on

correlates

to

ground

devices,

and

viewed

pin 1 as

to

a

on

Cylindrical

Cuvettes

=

Figure

4.

Cylindrical

Sample

Cells

Compartment

and

Filter

Plate

in

Multiple

SECTION

2

INSTALLATION

The

SPECTRONIC

relatively

corrosive

tions

must

ultimate

clean,

stable

vapors, and

be

avoided

instrument

70

spectrophotometer

environment.

wide

temperature/humidity

to

prevent

deterioration.

operates

Dust,

smoke,

reduced

best

in

vibration,

fluctua-

efficiency

and

SECTION

OPERATING

a

operation.

Voltage Alignment

PROCEDURES

All

instruments

Before

the

Adaptation, Meter

procedures

3

are

instrument

NOTE

shipped

is

Zero

given

ready

first

in

Section

for

115VAC + 10%

used,

perform

Adjustment,

4,

Maintenance.

and

the

Mirror

Line

If

at

any

or

excessive

in

Section

Operating

3.1

Setup

a.

Set

the

OFF.

b.

With

time

noise,

4.3. controls

power

Plug

the

power

the

instrument

perform

are

switch

instrument

switch

NOTE

the

shown

on

the

into a grounded

still

exhibits

Mirror

in

Figure

instrument

OFF,

reduced

Alignment

:

1.

check

that

sensitivity

procedure

rear

panel

outlet.

the

meter

to

reads

exactly

ment

procedure

c.

Set

the

power

minutes

3.2 a.

Set

b.

Insert a reference

control,

c.

Insert

meter.

for

warmup

Transmittance

the

desired

set

the

unknown

zero.

If

in

Section

switch

or

wavelength

blank

meter

not,

perform

4.2.

to

ON

before

using

Absorbance

with

and,

to

read

sample

the

and

the

using

100%T.

and

read

Meter

Zero

allow

at

instrument.

Measurement

wavelength

the

100%T/zero

%T

or A on

Adjust-

least

five

selector.

A

the

SECTION

4

MAINTENANCE

This

section

should

troubleshooting

an

authorized

4.1

Line

The

instrument

220,

or plug/socket strument

be

performed

Voltage

240

VAC,

just

describes

or

Bausch & Lomb

can

connector

in

front

all

by

the

repair

Adaptation

be

used

50/60

located

of

the

maintenance

operator.

tasks

should

service

with

Hz.

Adaptation

in

transformer

procedures

All

other

be

performed

representative.

line

voltages

is

the

interior

assembly.

that

maintenance,

only

by

of

100,

115,

made

with

of

the

in-

a

WARNING

Unplug

a.

Remove instrument

b.

Loosen

bracket

c.

Referring Note

that

used.

Remove

the

socket

d.

Replace

e.

Replace

f.

For

220-240V

0.7

amp

ment.

the

the down

to

the

250V

by

protector

lamp

the

instrument

lamp

access

loosening

protective

to

expose

Figure

lowest

the

Slo-Blo

5,

socket

plug

with a firm

corresponding

bracket.

access

operation,

fuse.

door

the

bracket

the

connector.

locate

position

door.

replace

(See

before

on

captive

screw

the

line

pulland

to

your

the

Section

proceding.

the

right

screw.

and

voltage

is a blank

line

voltage.

1.5

amp

4.6,

side

swing

connector.

push

fuse

Fuse

of

and

is

fully

with

Replace-

the

not

onto

Meter

Zero

Adjustment

Figure

which blade

c.

a

4.3. Correct

operates

the mator. instrument replaced, excessive

a.

b. c.

6.

Rear

Wall

Location

The

access

must

to

reach

Using a thin-blade

adjustment

is

exactly

and

shift

your

pointer

Turn

on

Use

Observe through

Mirror

lamp

the

image.

mirror

at

its

radiation

The

mirror

is

and

noise.

the

instrument

right

wavelength

the

of

hole

may

be

gently

the

adjustment

screw

over

the

viewing

Alignment

alignment

maximum

onto

first

installed,

if

the

side.

monochromator

rotating

alignment

of

Sample

Meter

Zero

NOTE

be

partially

pushed

screwdriver,

behind

0%T

instrument

the

line.

angle

ensures

sensitivity

the

entrance

whenever

on

and

selector

chopper

Screw

Compartment,

Adjustment

blocked

aside

screw.

access

Use

the

to

achieve

by

must

has

remove

to

set

entrance

blades

Screw

by a foam

with

the

carefully

hole

until

anti-parallax

asingle,

that

the

accurately

slit

of

the

be

checked

the

lamp

reduced

the

415nm

lamp

slit

and

sensitivity

or

visually

screwdriver

the

instrument

monochro-

assembly

access

higher. by

Showing

cushion

turn

the

pointer

mirror

coincident

imaging

when

the

is

or

door

sighting

center

Figure

4.2

Meter

When

before exactly required, in

the

rear

a.

Set

b.

Remove wall are hole.

5.

Interior

the

instrument

routine

over

is

wall

the

power

any

may

found,

of

Zero

operation,

the

0%T

made

of

switch

cells

have

the

adjustment

Lamp

Compartment

Adjustment

is

first

check

line

through

the

either

sample

from

an

(shown

the

one

installed,

that

with

power

access

compartment

in

Figure

sample

or

two

screw

and

the

meter

off.

Adjustment,

hole

(shown

area

7)

to

compartment.

access

holes;

is

behind

periodically

pointer

in

Figure

as

follows:

OFF.

The

if

two

holes

the

right-hand

rests

if

6)

rear

e

Figure

e

FUSE 1SA

SLO-BLO

a

7.

Holder

RATING

Instrument Fuse

Holder

Rear

and

Panel,

Power

Showing

Switch

Location

of

Line

the

lamp

filament

mirror

d.

e.

Replace

44

The has a first-surface must of

object tal excessive it

may

a.

b.

c.

d.

4.5

Lamp

Make lamp

The life sensitivity

output

recommended

The referring

a.

b.

c.

d.

e.

alignment

Set the

Carefully maximum readjust position

out.

fingerprints

Turn

Use swing

Mirror

lamp

not

be

including

mirror

be

cleaned

the

100%T/zero

adjust

meter

the

100%T/zero A control

the

mirror

the

lamp

Cleaning

mirror

touched

are

dust

using

instrument

mirror

Using a hand-operated

the

mirror

Rotate

position

Lamp

surface.

the

alignment

and

perform

Replacement

replacement

sure

the

instrument

assembly.

tungsten

rating

prealigned

Remove

instrument

Loosen

wires wires

Remove lamp

Being assembly.

tighten

Insert power

lamp

of

400

and

will

remain

that a spare

to

Figure 5 and

the

by

the

two

in

place

coming

the

lamp

assembly.

careful

Replace

it

down.

the

wires

thumbscrews

hours

the

assembly

lamp

loosening

lamp

on

from

not

screws.

f.

Perform

Mirror

image

lever

(shown

A

control

the

mirror

readout.

alignment

access

door.

CAUTION

(mounted

reflective

under

any

cleaning

particularly

alignment

surface

is

suspected

the

off

swabs,

following

and

air

lever

the

WARNING

involves

is

unplugged

assembly,

minimum.

lamp

position

almost

Alignment

lamp

using

access

power

the

lamp

the

instrument.

assembly

to

touch

the

lamp

from

the

(either

constant

the

on

the

entrance

in

Figure

for a 50-90%T

alignment

If

the

meter

for

lever

inside

the

coating;

circumstances

lens

damaging

of

causing

open

the

lever

(shown

toward

bulb,

the

blow

back

Mirror

contact

cat.

no. Because

employed,

Alignment

with

33-33-23,

over

assembly

can

be

quickly

the

following

door

on

captive

thumbscrews

assembly

thumbscrew.

the

bulb,

assembly

instrument

orientation)

procedure

slit

5).

lever

goes

50-90%T

for

maximum

lamp

this

mirror

with

tissue,

etc.

to

the

low

procedure:

lamp

access

in

door.

any

loose

toward

:

electrical

before

replacing

has a nominal

of

the

its

life

be

kept

replaced

procedure:

the

right

screw.

holding

and

remove

Discard

insert

the

thumbscrew

under

and

to

align

using

the

readout.

for

the

off-scale,

and

again

read-

access

door)

surface

any

kind

Acciden-

mirror.

sensitivity,

door.

Figure

procedure.

dust

its

original

5) to

from

wiring.

the

instrument's

usable

lamp

span.

It

available.

by

side

of

the

four

the

two

the

old

new

lamp

and

the

lamp

tighten

the

mirror.

If

is

4.6

Fuse

Replacement.

The

line

power

instrument

Because

instrument

rear

an

electronics,

replacement

authorized reguire cedure:

service

immediate

Hazardous

terminals.

proceeding.

a.

Remove rotating

b.

Remove value requires a 1.5

Turn

the

1/2

the

into

operation operation.

c.

Insert

the

cap

1/2

turn

clockwise.

d.

Connect

the replacement service

4.7 Cleaning

because also

with

cuvette.

nature

ammonium)

used

respectively.

removes

representative.

Glassware

of

cuvettes

any

contamination

because

material

subsequent

Cleaning

of

the

hydroxide

to

remove

Household

proteinaceous

inchromicacid

the

acid

should Because heat alteration

not

(with of

(Na,

of

generated

be

placed

To

prepare

stirring)

distilled

Cr,

07+

the

in

the

water

brown. Discard

Cuvettes

discarded absorbance protected

other

stood

outside

should

with

because

readings.

during

test

tubes

in a wire

of

the

be

kept

fuse

(shown

panel.

open

fuse

and

suggest

representative.

replacement,

WARNING

line

voltage

off

and

fuse-holder

turn

counterclockwise.

fuse

from

the

cap

amp

and a 0.7

exothermic

in

chromic

800ml

2H)

cuvettes

free

amp

and

fuse

line

cord

fuse

opens,

Maintenance

both

within

reagents

methods

contaminating

some

and bacterial

will

remove

be

handled

should

pathlength

hot

chromic

of

containing

0).

This

when

green

scratches

Round

cleaning

where

rack

that

may

of

fingerprints,

in

Figure

normally

we

do

not

recommend

that

the

problem

If,

however, use

is

present

unplug

cap

by

the

cap

and

retainer. This

250V

Slo-Blo

250V

Slo-Blo

into

the

holder

and

turn

refer

the

problem

inside

and

out

material

the

cell

or

standards

depend

material.

or

dilute

hydrochloric

acidic

bleach

acid

and

(undiluted

most

and

reaction

be

quickly

of

the

acid.

cleaning

types

disposed

of

cuvette.

concentrated

92g

of

cleaning

tinge

appears.

in

the

optical

will

test-tube

and

never

they

might

scratch

be

wiped

which

7)

is

located

results

from

operator

be

checked

circumstances

the

following

at

the

fuse

the

instrument

pressing

insert

fuse

spectrophotometer

fuse

for

fuse

for

and

lock

the

power

to

an

is

important

may

absorb

may

chemically

introduced

to

some

extent

Sodium

acid

basic

contaminants,

or

1:1)

contaminants.

of

contaminants,

of

the

acid

and

dissipated

Cuvettes

solution,

sulfuric

sodium

solution

acid

dichromate

should

path

cause

cuvettes

thrown

scratch

anomalous

into a bath with

each

their

with a soft

also

absorb

on

the

defective

fuse

by

an

pro-

holder before

down

and

of

proper

100-115V

220-240V

by

rotating

on.

If

the

authorized

not

only

light,

but

react

into

the

on

the

(or

may

be

effectively

Soaking

but

with

care.

water,

any

to

avoid

should

slowly

to

458ml

be

should

other,

faces.

tissue

light.

add

red-

be

or

The

and

5.1

Accessories

The

following

SPECTRONIC

a.

10-in.

Strip-Chart

115V

operation

Useful values rate

required

for

making a permanent

or

for

enzyme

to

SPECTRONIC

b.

DR-87

Digital

Hz

model

Features

with enable Patch DR-37

meter.

c.

Constant-Temperature

for

multiple

d.

Light

e.

SPECTRONIC

or

four-place

selectable

interfacing

cord,

Digital

Shield,

spectrophotometer

metric

f.

Filter TRONIC

g. h.

i.

Glassware:

Square

glass, Test

glass,

accuracy

Adaptor,

Standards.

Multiple Cuvette

compartment.

Sample

Holder,

cuvettes,

matched

tube

cuvettes,

double

33-17-75.

ACCESSORIES

accessories

70

spectrophotometer:

or

monitoring a changing

reactions.

connect

70

Readout,

cat.

decimal

cat.

no.

Readout

sample

cat.

Standards,

and

cat.

Compartment, cat.

set

stopper,

are

available

Recorder,

cat.

no.

the

cat.

39-11-30

Patch

cord,

strip-chart

spectrophotometer.

cat.

no.

33-30-93

LED

display

point.

BCD

with

data

printers,

33-26-71,

compartment.

no.

33-30-22,

accessory

Cuvette

cat.

is

for

no.

performance.

optical

10mm

of 2 in

selected,

alignment.

no.

33-30-27,

no.

33-30-15,

pathlength,

case;

matched

cat.

10mm

for

no.

39-11-20

for

220-240V

record

value,

cat.

33-30-92

for

230VAC,

of

photometric

output

required

to

the

Holder,

extra-long

33-31-50,

Test

0%T,

for

cat.

no.

for

45mm

no.

33-17-09.

pathlength,

set

of 2 in

use

with

for

operation.

of

photometric

such

as

no.

33-26-74,

recorder

for

115V

provided

computers,

to

connect

spectrophoto-

cat.

no.

33-30-14,

test

for

checking

spectrophoto-

use

with

33-30-02.

multiple

tall,

case;

cat.

SECTION

AND

the

100-

time-

is

to

the

AC,

60

50

Hz.

values

to

etc.

the

tubes.

SPEC-

sample

optical

no.

5

REPLACEMENT

Test tube tall,

optical

Cylindrical stopper,

Cylindrical

stopper, SPECTRONIC

js

Water

k.

5.2

ab

.

реа

.

Technology

Replacement

Universal

Blue

Phototube,

Red

Phototube,

Tungsten

pkg.

of

Chart

paper

39-11-30:

5

div/inch,

cat.

no.

6

div/inch,

cat.

no.

Pens

for

P-65, 2 red, 2 black,

P-68, 1 black, 1 red, 1 blue, 1 green, P-69, 4 black,

P-70, 4 red, P-71, 4 green, P-72, 4 blue,

SPECTRONIC

10001.

PARTS

cuvettes,

glass,

cells,

matched

cells,

matched

Test

Lamp.

2,

cat.

no.

for

single

39-11-44;

single

39-11-45;

recorder,

cat.

selected,

box

of

12,

50mm

pathlength,

set

of 2 in

100mm

70

Parts

Tube

range 325-625

range

set

of 2 in

Service

Manual,

Holder,

625-925

28V,

pathlength,

0.93A,

33-33-28. recorder,

roll,

cat. no.

box

of

24

roll,

cat.

box

of

24

cat.

no.

cat.

no.

39-11-69.

no.

39-11-70.

no.

39-11-71.

no.

89-11-72.

70

Operator’s

20mm

cat.

no.

case;

Manual,

cat.

no.

cat.

nm,

mounted

cat.

no.

39-11-42;

rolls,

no.

39-11-43;

rolls,

39-11-20

39-11-65.

Manual,

pathlength,

33-17-77.

optical

cat.

no.

33-17-82.

optical

cat.

no.

33-17-82.

cat.

no.

331015-10030.

no.

33-30-30.

cat.

no.

cat.

no.

and

39-11-20

cat.

no.

39-11-46.

cat.

no.

39-11-47.

or

cat.

no.

cat.

88mm

glass,

double

glass,

double

333041-10020.

33-30-56.

33-30-57.

prefocused,

or

cat.

box

of 4 rolls,

box

of 4 rolls,

no.

39-11-30:

39-11-68.

no.

333041-

no.

Bausch and Lomb Spectronic 70 User manual

Specifications and Main Features

Frequently Asked Questions

User Manual