Bausch and Lomb Spectronic 20 User manual

SECTION
1
DESCRIPTION
Figure
SPECTRONIC® single-beam range
of
20nm
is
The accessory the
capability,
wavelength
wavelength
If
this SPECTRONIC cedures this
manual
models,
333171-10020),
of
SPECTRONIC
SPECIFICATIONS
Wavelength
Standard Red
Phototube
Wide
Range
Spectral
1.
SPECTRONIC
spectrophotometer
340nm
constant
basic
by
without
consult
Slit
to
over
wavelength
adding
phototube
(cat.
provides
changing
Filter
in
ranges,
calibration
manual
20
basically
is
written.
SPECTRONIC
which
Range
Phototube
Phototube
Width
20
20
(cat.
with
950nm.
an
nominal
the
entire
range.
of
340nm
infrared
(requires
33-29-89)
continuous
many
in
addition
filter.
is
being
spectrophotometer,
contains
20
filter
filter
(cat.
cases,
used
same
replacement
detailed
models.
340nm 600nm 400nm 20nm
cat.
or
of
to
providing a simple-to-use
as
Model
20
Spectrophotometer
33-31-72),
an
to
600nm
(cat.
filter operation phototube.
33-31-28)
extending
to
Service
to
600nm
to
950nm
to
700nm
Figure
overall
spectral
33-29-72). A special
operate
33-31-72
technical
wavelength
slit
is
extended
33-29-18)
(cat.
33-29-92)
from
400nm
also
provides
an
operational
parts
Manual
description
1,
width
overall
older
pro-
which
earlier
(cat.
of
to
to
Wavelength Wavelength Stray
Photometric
Photometric Photometric Photometric
100%T
Accessory
Power
Size
Weight
Accessories
Bausch & Lomb fications development.
Accuracy Readability
Radiant
Range
Readability Noise Stability
Drift
Output
Requirements
Supplied
as
part
Energy
Level
reserves
of
on-going
41.3cm
33.0cm (16.25” W x
7.9kg
Box
X-in.
Manual,
2.5nm
1.0nm
(1/5
division)
Less
than
0.5%T priate stray filter
(available
filter
kit,
cat.
0%
to
100%T
0A
to2.0A
0.5%T +
0.5%T
1.0%T/Hour
OVDC 100, 115,
(Non-linear
to
1.0VDC
220,
90VA
W x 21.0cm H x
D
(17.4
Ibs.)
of
twelve
adapter,
dust
right
program
with
radiant
in
accessory
33-31-28).
240V
8.25” H x
4-in.
test
Operator's
cover.
to
change
of
appro-
energy
scale)
50/60
D)
tubes,
speci-
product
1.2
OPERATING
Figure 2 shows
three
main Control, Absorbance located Figure
1.2.1 Readings
transmittance
If
Troubleshooting
1.2.2
1.2.3
The
Control
Power
Control.
on
3.
Meter
the
meter
Wavelength
wavelength
of
on
scale
of
instrument’s
340nm - 600nm
Power
ON-OFF
Switch/Zero
knob.
FEATURES
features
controls
underside
taken
or
malfunctions,
wavelength
is
range
main
Switch/Zero
directly
absorbance
Guide.
Control
control
instrument.
color-coded
phototube:
range,
of
gradations
power
of
your
spectrophotometer.
routine operation
Control
accessory
of
mode.
refer
selects
scale
to
correspond
optional
Control
switch
Analog
instrument,
from
to
Table
desired
selected
in
window
black red
gradations
in
intervals.
is
operated
Wavelength
Transmittance/
Output
meter
wavelength
to
gradations
phototube/filter
Jack
as
shown
in
3,
Operator's
:
analytical
next
operating
by the
either
to
Zero
is
in
is
Zero
Control
WHEN THE
1.2.4
This blank
THE
ADAPTER
Transmittance/Absorbance
control
reference
WHENEVER
BEEN
length
100%T
(and
1.2.5
Accessory
analog
recessed Figure
of
output
instrument
To
make
3.
is
TEST
COVER
is
used
THE
CHANGED.
an
extended
0%T)
readout
Output
output
signal
at
100%T.
readout
signal
readout
output
manufacturer's
is
only
within
adjustment,
control
marked
used
to
meter
TUBE
to
solution.
ADAPTER
IS
CLOSED.
meter
IT
Control
to
MUST
ANALYTICAL
When
operating
period
of
time,
periodically
readjust
Jack
level
is
factory-set
To
provide
devices (recorders,
is
devices
signal
to
compatibility
adjustable
which
be
recognized
range.
use a small
Analog
from
Output
to a 0%T
IS
EMPTY
100%T
(0.04)
BE
WAVELENGTH
at a fixed
if
necessary.
to
approximately
digital
require
adjusted
display
to
slightly
output
warranty
screwdriver
Adjustment
readout
AND
with
RESET
wave-
check
with a wide
units,
at
more
to
VDC.
signal
covers
on
in
a
PLE
COMPARTMENT
SWITCH/ZERO
CONTROL
Figure
2.
Key
Operating
Features
LAMP
&
PHOTOTUBE
ACCESS
DOOR
ANALOG
VOLTAGE
DER
Figure
3.
Bottom
View
THIS
SWITCH
PLATE)
ANALOG
ADAPTER
OUTPUT
OUTPUT
le
ADJUSTMENT
WRENCH
J
a
Oe
ee
AO
ENVIRONMENT
SPECTRONIC
as
as
possible
or
electrical
instrument
dust,
corrosive
In
addition,
hinder
former is Rochester, voltage
instrument
accessible the
a. b.
flow
LINE
VOLTAGE
spectrophotometer
for
operation
set
to
operate
New
selection
to
by
instrument
UNPLUG
Tilt Remove located
up
THE
in
3.
c.
switch
d.
Replace required.
e.
If
the
converting
changing
1.
Remove
expose meter.
2.
Replace the
labels
supplied
20
spectrophotometer
from
strong
apparatus
should
gases,
there
under
be
and
should
generating
installed
severe
be
around
CONVERSION
contains a multiple-tap
on
100, 115,
at
115
VAC
York,
USA.
switch,
removing a cover
(see
shown
available
Figure
3).
CAUTION
INSTRUMENT
unit
on
lower
to
cover
to
Line
control
fuse
holder
ampere
screws
center
proper
plate
220
Voltage
knobs,
at
ampere
SLO-BLO
to
holding
and
or
magnetic
vibrations.
no
obstructions
220,
when
If
conversion
in
line
plate
BEFORE
back
of
voltage
change
240
VAC
Selection
sample
the
rear
SLO-BLO
250 V fuse
fuse
block
should or
high
in
an
area
instrument.
240
VAC,
it
leaves
Figure
4,
voltage.
located
panel.
switch
base,
shown
position.
the
line
operation,
Switch:
adapter
of
the
250 V fuse
supplied;
INSTALLATION
be
placed
electric
frequency
the
is
necessary, will
on
PROCEEDING
spectrophoto-
to
fields, fields.
that
is
free
which
power
50/60
factory
adapt
switch
the
bottom
cover
plate
in
Figure
cord
plug
after
cover
with
apply
the serial
SECTION
of
might
trans-
Hz.
It
in
the
the
is
of
if
to
2
3.
Figure
number
Replace
ment
4.
Line
access
plate
cover,
is
Voltage
ready
plate
on
knobs
Selection
shown
bottom
and
operation
in
of
sample
Switch
Figure
instrument.
adapter.
at
(located
3)
or
The
instru-
VAC.
under
SECTION
OPERATING
3
PROCEDURES
The
instrument
factory
operate
Section
SETUP
To
help
your a.
Install
b.
Select
ο.
Turn warm-up.
3.1.1 In
addition supplied available. shown
screw
wrench
Test
Y-in.
in
at
100,
2.2.
ensure
spectrophotometer:
reguired
on
Glassware
to
with
in
Table
on
the
clipped
Table
Glassware
Tubes:
dia.,
12,
cat.
is
set
to
Rochester,
220,
or
240
uncomplicated
adapter
install
appropriate phototube
Power
optical
no.
Switch/Zero
Selection
standard
instrument,
sample
inner
1.
to
To
i.
adapter
change
wall
bottom
Glassware
glass,
33-17-80.
CAUTION
operate
York,
V.
If
adjustment
%-in.
several
must
adapters,
of
the
of
at
115
VAC
adjustment
select
sample
Control
Sampling
test
tube and
types
match
loosen
adapter
instrument
and
Adapters
Adapter
33-31-27
(Supplied
instrument)
when
it
leaves
be
is
required,
P
operation
holder.
filter.
for a 15-minute
Options
%-in.
adapter
of
glassware
glassware,
small
using
the
adapter
(see
Figure
Cat.
No
with
see
to
of
as
To
TURN
.
Tilt
.
Loosen
Figure
.
Remove
e.
Install insert
prints.
Close
IS
change
up
the
the
3) the
into
tube
door
the
unit
thumbscrew
phototube
tube the
ESSENTIAL
Y < РНОТОТУВЕ
phototube:
UNPLUG
and
open
holder
filter
securely
FOR
set
on
on
door.
using
plastic
on
must
PROPER
SK
a
©
its
back.
the
lamp
plastic
gripper.
inside
be
clean
fasten
OPERATION.
SOURCE
INSTRUMENT.
access
gripper
filter
of
door
free
thumbscrew.
LAMP
*
FILTER
door
as
is
required,
(see
of
HOLDER
(see
4
an
aid”
Figure finger-
THIS
%-in.
dia.,
of
12,
cat.
1-in. dia., of
cat.
Cuvettes:
Square, optical
case,
cat.
*Requires
3.1.2
Phototubes
As
shown determined installed
in
Range
340-600nm 600-950nm 400-700nm
appropriate
33-31-28.
optical
no.
33-17-81.
optical
33-17-82.
%-in.
pathlength,
glass,
no.
33-17-01.
Light
in
Table
by the
instrument.
Table
glass,
glass,
of 2 in
Shield,
particular
filter
cat.
2,
2.
Phototube
Phototube
33-29-71 33-29-72 33-29-89
from accessory
33-29-31*
33-29-30*
33-29-49
33-29-32.
operating
phototube-filter
Cat.
wavelength
Options
No.
combination
Filter
Cat.
None
33-29-18* 33-29-92*
Filter
range
No.
Required
Kit,
cat.
is
Figure
5.
Lamp
Compartment
3.2
SAMPLE
seguence
a.
Select With
b.
samplecompartment
Zero
Control
İnsert set
Transmittance/ A bsorbance
.
Insert
read
measurement
or
absorbance.
added
be
attached
reference
MEASUREMENT
sample
wavelength
so
that
reference
unknown
sample
convenience,
to
to
using
blank
from
right
proper
measurement
Wavelength
empty
meter
reads
into
sample
Control
into
sample
meter
in
self-stick
side
of
controls
Control.
coter
zero.
compartment
compartment
percent
meter
closed,
adjust
to
100%T
(0.0A).
transmittance
instruction
bezel*to
procedures.
provide
plate
Correct laboratory
are
necessary
a.
solutions
b.
sample
с.
For
greater
with
adapter,
Problem
1.
Instrument does
work.
2.
Meter
does
zero.
3.
Meter
reading
drifts
for
successful
must
holders
accuracy,
cat.
a.
b.
с.
d.
e.
a.
b.
c.
d.
e.
a.
b.
c.
d.
e.
f.
g.
NOTE
procedures
use
be
free
must
be
use
square
33-29-49.
Possible
Power
line
connected
outlet.
Dead let.
Source burned
Phototube fective.
Defective tronic
nent.
Sample
partment
closed.
Occluder
ing. Lamp
door
closed. Phototube
fective.
Defective
tronic
nent.
Poor
sampling
technique.
Fumes sample.
Excessive
voltage
tion. Wrong
line
setting.
Source fective.
Phototube fective.
Meter tive.
and
of
of
bubbles.
at
least
Cause
cord
power
lamp
out.
elec-
compo-
com-
cover
bind-
access
tightly
elec-
compo-
from
line
varia-
volt-
lamp
defec-
analytical
your
spectrophotometer.
half
full.
cuvettes,
to
cat.
Table
Plug
cord.
out-
Replace
lamp.
Replace quired.
Refer manual
center.
Check tion.
Replace quired.
Refer
manual
center. Eliminate
or
particles
tion.
Remove
immediately
analysis.
Check
grounding.
Reset
Selection
Replace lamp.
Replace quired.
Refer manual center.
techniques
i
no.
33-17-01,
3.
Operators
Remedy
in
power
with
as
to
service
or
service
occluder
as
to
service
or
service
bubbles
in
sample
voltage
Line
Voltage
Switch.
with
as
to
service
or
service
line
solu-
after
and
d.
optimum
ensure
mark During
e
an
occasional
causes
Troubleshooting
Problem
4.
Cannot
100%T
5.
Readings
repeatable
even
though
meter
reading
is
zero
100%T
is
set
correctly.
performance
that
on
adapter.
extended
of
meter
Guide
(0.0A).
control
index
operation
check
drift
Possible
.
Defective
tronic
nent.
.
Occluder
.
Sample
not
serted dapter.
.
Improper installed.
.
Source weak.
.
Wrong
age
.
Phototube
.
Error length tion.
.
Defective
tronic nent.
.
Loose cess
.
Loose holder
.
Poor
technigue.
.
Test
tion
ing.
.
Meter
with
test
mark
on
at a fixed
100%T
listed
in
Cause
elec-
compo-
closed.
holder
fully
in-
into
a-
filter
Lamp
line
volt-
setting.
weak.
in
wave-
calibra-
elec-
compo-
lamp
door.
sample
adapter.
analytical
tube
posi-
not
repeat-
sticking.
tube
sample
tube aligns with
wavelength,
meter
Table
drift.
3.
Refer manual
center.
Install
sample
Remedy
to
service
or
test
compart-
ment. Insert
fully.
Remove
or
filter.
Replace
lamp.
Reset Selection
Replace
quired.
Check
Refer
manual
center.
Tighten
screw.
Tighten
Clean dirty remove
ete.
Always fiducial actly place tube into
with
Line
Switch.
as
calibration.
to
service
or
thumb-
or
replace
test
bubbles,
position
line
when
is
inserted
adapter. lightly
correction.
holders,
make
Possible
service
tube
in
change
Voltage
service
screw.
tubes;
in
same
test
pos-
SECTION
MAINTENANCE
4
Because
functional
routine
ment phototube checks
as
well
If the and beyond qualified
to
Section
of
design
customer
of
the
6.5V,
(Table
for
wavelength
as
change
replacement
Service
instrument,
Manual.
scope
dealer
4.6,
or
Service
SPECTRONIC (cat.
333171-10020)
qualified
b.
c.
d.
e.
f.
4.2
To procedure
4.3
Under
to
perform
LAMP
TURN
Tilt
Loosen the
Using
REPLACEMENT
OFF & UNPLUG
up
the
door.
finger mounting clockwise
To
install
socket
Clean
lamp,
IS
ESSENTIAL
PHOTOTUBE
replace
in
WAVELENGTH
normal
unit
thumbscrew
pressure, flange and
phototube,
Section
operating
spectrophotometer
indefinitely. or
other
of
a.
Cobalt
b.
Didymium
abuse,
three
Solution
If
the wavelength
methods:
filter
33-31-28).
SPECTRONIC
tested
maintenance
2.75A
source
2).
The
operator
calibration
operating
parts
needed, a complete
protection
instrument
of
this
section
Bausch & Lomb
Procedure.
NOTE
20
Spectrophotometer
is
available
electronic
and
set
on
on
press
(see
Figure
remove.
lamp,
rotate
lamp
close
door,
FOR
PROPER
REPLACEMENT
refer
8.1.2
of
this
CALIBRATION
condition
should
instrument
Check.
from
retain
performance
20
field-proven
has
been
lamp
(cat.
can
and
line
voltage.
repair
should
service
servicing.
INSTRUMENT.
its
back.
lamp
lamp
Rotate
LIGHT
clockwise
tighten
OPERATION.
to
phototube
manual.
wavelength
is
subjected
accessory
spectrophotometer’s
reliability,
reduced
also
photometric
of
to
access
socket the
finger
until
to
no.
33-33-85)
perform
accuracy,
list
is
both
or
maintenance
be
performed
personnel.
Service
those
technically
door
toward
lamp
pressure
secure.
securely.
replace-
and
routine
found
operator
Refer
Manual
open
counter-
THIS
oT
CHECK
SPECTRONIC
accuracy
to a severe
may
be
Filter
checked
(cat.
shock
in
by
on
20
by
c.
Wavelength dards
(cat.
An
explanation
Instructions
Standards
are
SPECTRONIC Canada
4.3.1 First
a.
b.
only.
Cobalt
prepare a stock
1-liter
Add
slowly
chloric
distilled In a 1-liter
acid
chloride acid.
Make
to
obtain
To
perform
a.
Turn
power
b.
Wavelength
c.
Withsample Zero
Control
d.
Insert sample
Control
e.
Replace
glassware compartment
at
distilled
chloride
Figure
6.
Lamp
Accuracy
33-31-50).
on
of
found
Standards
Solution
volumetric
and
(ACS
water
to
volumetric
(ACS
grade).
to
volume
chloride
cobalt
compartment
until
100%T.
stock
solution.
Replacement
Test
of
cobalt
didymium
in
the
user's
Check
cobalt
solution
flask,
with
caution,
grade).
obtain
1%
hydrochloric
flask,
Dissolve
with
1%
stock
solution.
solution check:
Allow
15-minute
Control
at
empty
meter
reads
filled
with
and
set
water
with Read
from
SPECTRONIC
solution
filter
manual
available
as
place
10
ml
place
in
hydrochloric
check
SPECTRONIC
for
each
in
follows:
ml
distilled
concentrated
make
to
volume
acid
22-23
1%
hydrochloric acid
follows.
accessory.
U.S.A.
solution.
gm
solution
warm-up.
cover
closed,
zero.
distilled
Transmittance/Absorbance
glassware
%T
on
water
containing
meter.
into
Stan-
water.
hydro-
with
cobalt
adjust
the
cobalt
uk
Repeat
instrument
ance
515nm.
unimportant.
4.3.2
If
Section not
44
If
the
first proper
Figure
Accura:y/Linearity
(cat. formance
preparation
a.
Install
b.
Turn ment
steps
is
(minimum
(See
Figure
Wavelength Adjustment
wavelength
4.5,
Service
recommended.
PHOTOMETRIC
photometric
check
your
operation
alternate
7.
Example
Cobalt
33-31-50)
of
your
mentioned
required
on
Power
15
in
minutes.
c
through
proper
e
calibration
transmittance)
7.)
The
Calibration
accuracy
Procedure.
LINEARITY
linearity
analytical
is
still
of
in
N
|
510nm
of
Wavelength
Solution
Test
from
to
test
instrument.
method
uses
in
Section
adapter
Switch/Zero
at
505,
510,
when
occurs
specific
is
Customer
the
instrument
procedure
doubt,
7
Calibration
SPECTRONIC
evaluate
cobält
4.3.1:
select
Control
515,
and
maximum
between
absorbance
of
tolerance,
recalibration
CHECK
is
and
use
the
Check
photometric
chloride
sample
stock
holder.
warm-up
520nm.
questionable,
technique.
Photometric
absorb-
505
values
refer
The
and
are
to
is
If
Using
Standards
per-
solution
instru-
c. d.
e.
f.
g.
h.
4.5
If
be trained
center.
If warranty, purchased.
If
Lomb instrument Systems
code
If
a.
Wrap
in a crush-resistant
absorbing
b.
Include a detailed fastened
include
department
information
quired
c.
Mark FIRST First
carton
Wavelength
Withsample Zero
Control
Insert
sample
Absorbance
Remove sample
ANCE
Carefully
hydrochloric
Insert
must
corrected
it
distilled
of
value
be % of
SERVICE
instrument
specialists
To
avoid
SPECTRONIC
contact
warranty
Field
Service
or
Division,
716,
338-8245
is
necessary
spectrophotometer
to
repairs
on
CLASS
class
will
cobalt
dilute
cobalt
by
call
material
postage
be
Control
compartment
until
meter
of
distilled
Control
on
acid.
stock
operator
name
head
will
shipping
at
100%T.
water
chloride
meter.
cobalt
chloride
dilution
solution
PROCEDURE
should
unnecessary
instrument,
promptly
accepted
develop a malfunction
maintenance,
at
your
dealer
dealer
expired,
Center
Service Bausch & Lomb, or
338-8306.
to
ship
the
carton
to
prevent
letter
most
familiar
enable
container:
LETTER
will
be
at
at
510nm.
empty
reads
zero.
water
and
insert
glassware
stock
solution.
stock
solution
of
step
g.
ABSORBANCE
(step
f), + 2
it
or
Bausch & Lomb
delays:
20
spectrophotometer
from
whom
given
in
list
Department,
Rochester,
instrument:
in
plastic,
with
inside
describing
phone
service
at
ENCLOSED.
required
standard
then
at
least 3 inches
transit
number
with
personnel
least
expense.
only
mail
cover
closed,
set
Transmittance/
containing
Note
ABSORB-
1:1
divisions.
that
can
be
serviced
is
instrument
contact
damage.
shipping
of
on
Bausch
packed
Instruments
N.Y., area
pack
carefully
of
trouble.
person
problem.
to
make
letter.
rates.
adjust
with
1%
value
cannot
by
service
under
with
shock
carton,
Please
or
This
&
&
following
SPECTRONIC
SPECTRONIC
a.
reliable
0%T,
wavelength
metric
adapter,
33-29-49.
Filter SPECTRONIC
Filter
energy/2nd
10-in.
Includes 1 roll
Manual.
10-in.
Strip-Chart
Includes 1 roll
Manual. Patch
Recorder
TRONIC
DR-37
33-30-92.
accessories
20
spectrophotometer:
Standards,
to
test
accuracy,
accuracy
cat. no.
Adapter,
33-31-26,
cat.
Standards.
Kit,
cat. no.
order
filters, 1 didymium
Strip-Chart
of
chart
of
chart
Cord,
cat.
(cat.
33-11-20
20
spectrophotometer.
Digital
Readout
available
evaluate
stray
optical
and
33-31-26.
33-31-28.
Recorder,
paper, 4 ink
Recorder,
paper, 4 ink
33-26-74.
Accessory,
cat.
no.
33-31-50.
instrument
radiant
alignment;
cuvette
performance:
energy,
requires
holder,
Required
Includes 4 stray
filter.
V,
cat.
cartridges,
V,
cat.
cartridges,
Connects
or
39-11-30)
115
V.
to
50-60
SECTION
ACCESSORIES
with your
Quick,
photo-
filter
cat. no.
with
radiant
39-11-20.
Operator’s
39-11-30.
Operator’s
Strip-Chart
SPEC-
Hz,
cat.
5
h.
DR-837
Readout
meter. Step-down
to
.
SPECTRONIC
Industrial tions. Alloys,
332905-10030;
10030.
332902-10030.
.
Water grouping
wastewater
n.
Accurate
Digital
33-30-93.
Cord,
115
V.
Aluminum
Ring
Technology
Readout
cat. no.
Accessory
transformer,
20
Service
Methods
Alloys,
cat.
332904-10030;
Gold
binder
of
EPA-recommended
analyses.
Water
Testing
Accessory,
33-26-71.
to
SPECTRONIC
cat.
Manual,
Manual.
cat.
and
Gold
to
hold
Manual,
Manual,
Connects
no.
33-32-97.
cat.
Purchase
Copper
Alloys,
or
cat.
methods
cat.
230
V,
50-60
DR-87
20
spectrophoto-
Converts
no.
333171-10020.
in
separate
332903-10030;
Alloys,
cat.
no.
sections,
331015-10030.
water
330935-10030.
Hz,
cat.
Digital
220
V
sec-
Steel
cat.
332906-
cat.
A
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