BAS LC-22C Instruction Manual

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Page 1

LC-22C

March, 2000

MF-9010 INSTRUCTION MANUAL

Temperature Controllers

Bioanalytical Systems, Inc

2701 Kent Avenue West Lafayette Indiana 47906

for Liquid Chromatography

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MANUFACTURER’S NOTE

The BAS logois a registered trademarkof Bioanalytical Systems, Inc.

No portion of this manual may be reproduced without express, written permissionof Bioanalytical Systems, Inc.

Bioanalytical Systems, Inc.

2701 Kent Avenue

West Lafayette, IN USA 47906-1382

Phone: (765) 463-4527

Fax: (765) 497-1102

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Section 1. PREFACE

The Bioanalytical Systems LC-22CTemperature Controller andLC-23A and LC-23C Column Heating Compartments are versatile tools for the experimenter who desires precise, reproducible temperature control for liquidchromatography.

The LC-22C DualChannel Temperature Controller is a general purposeinstrument capable of regulating two independentdevices, such as heatingmantles, heat tapes, electrochemical cell preheaters, and column heating blocks. The temperature control point isset with a multi-turn front-panel potentiometer and thepower applied to the heating unit is regulated by a temperaturesensor located in thedevice being controlled. In heating devices of BAS manufacture, the temperature sensoris built into theinstrument. To control other devices, a universal probe (part no. EW-8116) is available forseparate purchase.

The LC-23A ColumnHeating Compartment is usedin conjunction with the LC-22C. This instrument is simple in design and extremelydurable. It accepts most commercially available 15, 25 or 30 cm analyticalcolumns. Asolid aluminum inner compartment, thermally isolated from the outer cover,encloses the entire column. A preheatercoil is included with each unit. The LC-23A can, in someinstances, be altered toaccommodate wider diameter columns. This alteration would involvean additional charge for customization. Ifyou require modifications, please contact BAS customer service for a quotation.

Figure 1. LC-22C Front Panel.

Temperature Controller

The LC-23C CartridgeColumn Heating Compartment accommodates two cartridge format columns up to15cm in length. The LC-23C canbe mounted within the BAS CC-5 Chromatography Compartment, which ispart of the BAS 480 Liquid Chromatographsand LC-44 Electrochemical Detectors. The LC-23C is also sold with orwithout the CC-5, the latter as anupgrade to existing BASdetectors or BAS 480 systems.

The Cell Preheateroption for BAS electrochemicaldetectorsis also controlled byan LC-22C. Thepreheater coil can accommodatethe heating of stainlesssteel tubing between the column andcell inlet.

LC-22C

DISPLAY

SELECT

SETPOINT HEATER 1

SETPOINT HEATER 2

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Section 2. POWER REQUIREMENTS LC-22C CONTROLLER

Section 2. POWER REQUIREMENTS

Figure 2. LC-22C Back Panel.

REMOTE

PROBE 1 PROBE 2

ADDRESS OUTPUT

GND

1234

21INH

GND

LC-22C Made in USA

HTR 2HTR 1 F1

LC-22C CONTROLLER This instrument can be operated from either115V/50-60Hz or 230V/50-60Hz. The line

voltage selector for 115V or 230V operationis on the back chassis panel. The line voltage must be selectedbefore applying power to the controller.

A 3-prong powercable on the LC-22C must be connectedto a standard, groundedpower outlet for safety. Ifthe instrument is operatedfrom a poweroutlet without a ground connection, an appropriate adapter should be used andthe ground connection of this adapter should be securelyfastened to an earth ground.

LC-23A and LC-23C HEATING COMPARTMENTS

The LC-23A andLC-23C compartments are designedspecificallyfor use withthe LC-22C Controller. Two cables (one for heater powerand the other for the temperature sensor) connect to the LC-22C to each heatingdevice. If you havepurchased a heating compartment for use with an older model LC-22A, you may be unable to make the connection directly to thecontroller without an additional adapter. A change indesign converted this from a 3-prong male plugto the current female plug. The adapter (EF-1575) is available from BAS at an additionalcharge.

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LC-22C CONTROLLER Section 3. INSTALLATION

Section 3. INSTALLATION

LC-22C CONTROLLER This section concerns installation forstand-alone operation of the LC-22C with the optional

universal probe. For operationof the other accessories,see subsequent sections.

1. Use the cableadapter (EF-1575) to convertthe special safety receptacle onthe back panel of the LC-22Cto a standard 3-prong socket. Plug the deviceto be controlled (e.g., heating mantle, tape, etc.)into this socket. If you only wish to measure temperature and do notneed to control it, you can skipthis step.

2. Plug the cableleads from the stainlesssteel UNIVERSAL PROBE (EW-8116)into the red, yellow, and black jackson the backpanel of the LC-22C labeled “PROBE 1"or ”PROBE 2". The black jackprovides grounding for the instrument and must be connected for safety purposes.

3. The liquid probe is usually accurate to within 1°C of theactual set-point when shipped. The reproducibility or precisionof the temperature set-point is± 0.1°C regardlessof

the actual temperature. Ifgreater accuracy is desired,follow the calibration procedure.

To performthe calibration, first remove the top cover of the LC-22C byremoving the four screws located on the sides ofthe top cover. Since both channel1 and channel 2 are identical, calibrating instructions willbe given for channel1 adjustments, while channel 2 adjustments willappear in parentheses ().

To calibratethe probe, place itin an ice bath and connect theprobe leads to the “PROBE 1" (”PROBE 2") jacks on the back panel. Turn the main poweron and rotate the display selector knobto “SENS 1"(”SENS 2"). Usinga small screwdriver, rotate the adjusting screw on the top ofthe potentiometer R45 (R56) for a readingof 00.0° on the front-panel digitalreadout. Remove the probe from the ice bath. If a two-point calibration is desired, goto step 5.

4. This completes the single-pointcalibrationof the liquid probe. The top cover should be reinstalled using the fourretaining screws on thesides of the cover.

5. A two-point calibration canbe made byplacing the probe inboiling water and adjusting R46 (R55) firstto a display readingof 99.9, then to 00.0 (indicating 100.0°C) withthe center decimal point on. There could be a very slight interaction between thefreezing and boiling point adjustments,so additional adjustments to R45and R46 (R56and R55) could beneeded to obtain thehighest degree of accuracy. Remove theprobe from the hightemperature bath.

6. This completes the two-pointcalibration of the liquidprobe. The top cover should be reinstalled using the fourretaining screws on thesides of the cover.

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Section 3. INSTALLATION LC-23A COLUMN HEATING COMPARTMENT

LC-23A COLUMN HEATING COMPARTMENT

1. Place the LC-23Aheating Compartment in thedesired location. Six holes withcaptive 6-32 threads are provided for secure mountingon your liquid chromatograph,if desired. The holes are positioned to fit earlymodel (pre 1986) BAS liquid chromatography support stands.

2. Connect the LC-23A heater power cable tothe power socket on the back panelof the LC-22C Controller.

3. Connect the red, black and yellow plugson the probe cableto the jacks marked “PROBE 1" or ”PROBE 2" on the LC-22C back panel.

4. Remove the coveron the LC-23A Column Heater by unscrewingthe two cap screws. Place the columnin the recessed cavityand route the inletand outlet tubing outof the top and bottomslots. A special preheater block(EW-8146) is also supplied. Under certain circumstances, dramatic bandbroadeningmay result ifthe preheater is not utilized. Typically thisbecomes obvious when the temperature differential between ambient and thedesired set point exceeds20-25°C.

If you planto use several differentstyles of column endfittings, itis best to use removable plastic fittings. BAS recommends a 2-piece PEEK finger-tightseal as provided in BAS part no. MR-4409 (this includes a nut and ferrule). One pieceplastic fittings tend to loosenat higher temperatures.

Figure 3. Position of Mounting Points on LC-23A Heater.

2.01 5.00 6.00 3.37

Figure 4. LC-23A Column Heater with LC-23PH Preheater Installation.

39.37cm

10.79cm

SENSOR

LOCATION

ADAPTER FOR

25-30cm COLUMNS

4.12cm

COLUMN

1.93cm

PREHEATER

1.91 x 2.54cm INLET DEPTH

1.89cm

9.36cm

CHANNEL WIDTH

0.685cm HEATER

BLOCK

4.92cm

8.89cm

OUTLET DEPTH

1.85cm

2.03cm

0.50

0.75

0.50

2.54cm

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LC-23C COLUMN HEATING COMPARTMENT Section 3. INSTALLATION

LC-23C COLUMN HEATING COMPARTMENT

The LC-23C isspecifically designed to fit withinthe BAS CC-5Column Compartment. The CC-5 is pre-drilled to accept a Rheodyneinjectionvalve on the front panel. It has several grooves on bothsides for convenient access to external detectors.

If you areinstalling the LC-23C asan upgrade to a BAS electrochemical detector or BAS 480 Liquid Chromatograph, andalready have a cell preheater installed in theCC-5, you may wish to remove it. Routing thetubing from the LC-23C to the cellpreheater and then to the detectoris difficult due to space limitations. The LC-23C alonewill provide satisfactory temperature control forthe electrochemical cell if you keep the coverclosed during operation.

Before installing the LC-23C,give some thought to the configurations of the column(s) you intend to use. Some configuration using compact BAS cartridge columnsare diagrammed in Figure 5. You can alsoleave the analytical column outsidethe LC-23C (at room temperature) and heat a post-column reactor (tubing coil,packed bed) within theLC-23C.

Figure 5. Combinations of Column Formats Within the LC-23C.

10cm

ANALYTICAL

COLUMN

15 OR 10cm

ANALYTICAL

COLUMN

3cm

POST-COLUMN

TO UV

DETECTOR

MIXING TEE

AND REAGENTS

ANALYTICAL

COLUMN

POST-COLUMN

REACTOR

15cm

ANALYTICAL

COLUMN

POST-COLUMN

REACTOR

ANALYTICAL

COLUMN AT

AMBIENT

10cm

ANALYTICAL

COLUMN

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Section 3. INSTALLATION LC-23CCOLUMNHEATING COMPARTMENT

Installing the LC-23C in a CC-5 Compartment

1. Lower the CC-5lid and remove the utility cover from the back panel of the CC-5 cabinet (four screws).

2. On some models,pressed-in, threaded nuts will be located on theoutside of the back panel (Figure 6). These must be removed. DO NOT REMOVE IFTHEY ARE ONTHE INSIDE OF THECABINET. To remove external nuts,screw a long machinescrew (6-32 thread) into the nut from the rear until it protrudes throughthe nut. Use pliersfor leverage and gently work the screw fromside to side until the nut comesloose.

3. Remove the LC-23Ccover and set aside. Push the heater block throughthe now open utility access in the CC-5 back panel. On some models, the two registrationposts on the CC-5 innerpanel will engage theLC-23C block for support.

4. Secure the LC-23Cto the cabinet usingfour 6-32 x 1/2" machine screws andfour 6-32

lock nuts. The lock nuts are notnecessary if pressed-in nutsare available on the inside of the CC-5 cabinet. The mountingplate should now beflush with the outsideof the back panel on theCC-5 cabinet.

Figure 6. Installation of the LC-23C in a BAS 480 or CC-5 Electrochemical Detector. (A) Location of four screws (B) Breaking off

the pressed-in nuts (C) Left Side View of Attachment Hardware.

SCREW

LOCATIONS

6-32 x 1/2" SCREW

(4 PLACES)

CC-5 REAR

6-32 LOCKNUT

(4 PLACES)

CC-5 FRONT

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LC-23C COLUMN HEATING COMPARTMENT Section 3. INSTALLATION

Making Plumbing Connections

For simple arrangements, use the innermost trough of the LC-23C. This locationminimizes detector and valve hookup tubing. If you are coupling columns together,the longer trough will be moreuseful.

A special coilof tubing is providedwith each LC-23C (see Figure 7). At your option, it may be installed between theinjection valve and column. The coilfunctions as a mobile phase preheater, so that large temperature gradients atthe head of the column are minimized. The coil may beremoved by unscrewing thecentral cap screw (5/32" hex).

It is advisable not to use apermanent ferrule on the outlet end of the preheater coil. A removable high-pressure fitting provides an equivalent seal without irreversible attachment. Use BAS part no. MR-4409.

Figure 7. Column Preheater Coil Installed in LC-23C. This Unit is Part of a BAS 480 Liquid Chromatography System with the

Electrochemical Cell and Cell Preheater Options Installed.

PREHEATER POWER

HIGHLOW

Leaks

Make sure thatall fittings are leak-tight atthe usual operating pressurebefore mounting the insulating top cover.

Insulating Top Cover

The U-shaped top cover is insulated on three sides with a foam polymer. The cover is asymmetric; the notch in the cover goesalong the right side. Mount the cover at therear first and thendrop the front inplace.

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Section 4. OPERATION TEMPERATURE MEASUREMENT/CONTROL MODE

Section 4. OPERATION

For purposes ofinstruction,we will reference channel1, although channel 2is identical.

1. With the heaterand probe cables disconnected, turnon the main power to the LC-22C.

2. Rotate the display selectorknob to illuminate “SET1" in the red display window.

3. Using the “SETPOINTHEATER 1" knob, select the desiredtemperature, which is between ambient and 99.9°C.

4. Turn offthe main power to the LC-22C and connect the heater to “HTR-1" and thered, yellow and black probe plugs to thesame color jacks of ”PROBE-1" on the back panel.

5. Turn onthe main powerto the LC-22C. The heater indicator LED will remain constantly illuminated until the setpoint is reached, thenit will dim or blink intermittently to indicate maintenance at thedesired temperature. You can also observe the measured probe temperature by changingthe display select switchto “SENS 1".

TEMPERATURE MEASUREMENT/CONTROL MODE

LC-23A or LC-23C Column Heaters and Electrochemical Cell Preheater

1. Ensure that the three (3) leads from the column heatersor cell preheater areplugged into the jacks marked“PROBE 1" or”PROBE 2"

2. Turn onthe LC-22C. Set the desired temperature (at least 5°C above ambient).

3. The measured temperature can be read onthe front panel digitaldisplay of the LC-22Cby using the display selectorknob. The temperature isalso available as an analog voltage at the screwterminals marked “OUTPUT + 1 -” or “OUTPUT + 2 -”, depending upon the channel used. Each 100 mV (0.1 volt) is equivalent to1°C. For example: +8.92 volts = +89.2°C

-0.36 volts = - 3.6°C

This voltage may be recorded using a strip chart recorder. Set the fullscale input of the recorder to10 volts and observe the marked polarityon the back panel.

4. The LED lightwill stay on continuously untilthe heater achieves thesetpoint and then will flash intermittently while maintaining thistemperature. The actual temperature (within the absolute accuracy ofthe probe) atany point in time will be displayed on the front panel digital display ofthe LC-22C or the recording device.

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TEMPERATURE MEASUREMENT/CONTROL MODE Section 4. OPERATION

Universal Liquid Probe

1. Ensure that the stainlesssteel probe is pluggedinto the jacks marked “PROBE 1" or ”PROBE 2" and observe the colorcode used on the jacks.

2. Turn themain power on to the LC-22C. Rotate the displayselector knob to “SENS 1" or ”SENS 2", depending upon which channelis being used for measurement. The digital display will readthe probe temperature in degrees C.

3. The measured temperatureis available as an analog voltage at the “OUTPUT” screw terminals on the back panel. This six positionterminal strip is detachable:pull straight back for release. Probe 1 outputs are the labeled “+ 1 -”,probe 2 outputsare labeled “+ 2 -” on the backpanel.

4. An analog voltage of 100 mV (0.1 volt) is equivalent to 1°C. For example: +8.92 volts = +89.2°C

-0.36 volts = - 3.6°C

This voltage may be recorded using a strip chart recorder. Set the fullscale input of the recorder to 10volts. Observe the marked polarity.

5. To controlthe temperature while measuringit, first turn offthe main power to the LC-22C. Connect the power cable of the heatingdevice (75 watts maximum)to the appropriate heater jack, i.e., “HTR 1" if ”PROBE 1" is the corresponding sensor channel.

Turn themain power on and select the correspondingchannel setpoint LED. Rotate the setpoint heater knob to obtain the desired controltemperature. The actual probe temperature can be observed by changing the display selector knob tothe controlled channel’s sensorLED, marked “SENS1" or ”SENS2".

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Section 5. SAFETY AND MAINTENANCE CHECKS

The LC-22C, LC-23A,LC-23C and cell preheaterare all essentially maintenance free instruments. BAS does advise that you attempt to keep these piecesin good condition by following these suggestions:

1. Avoid corrosive laboratory environments.

2. Don’t stack heavy items on signal cablesor allow them to become crimped duringuse or storage.

3. Clean up spills insidecolumn heaters immediately.

4. Avoid contact of organicsolvents with the insulating materialinside the LC-23C cover.

The Universal Probe is based on a reliable solid state devicecapable of generating a predictable current related tothe temperature of itsenvironment. To checkthe integrity of this probe, apply the following test:

1. Expose probe to ambient temperature and disconnect anyheating devices from the LC-22C.

2. Turn onthe LC-22C power.

3. Set the temperaturesetpoint to a valuegreater than ambient (allowat least 1°C difference). The “HTR” LED indicator on the LC-22C should be on continuously.

4. An open circuitanywhere in the probe,cable or across its input jacks will automatically shut down theheater circuit. This feature was incorporated to protect againstthermal “runaway” and should be tested periodically. Perform the following check:

A. Plugin the probe, exposeto ambient air temperatureand turn on the

power to theLC-22C.

B. Createan open circuit inthe probe by unplugingthe red or yellow lead

from the chassisjacks on the LC-22C. Using the digital display selector knob, illuminate thetested channel “SENS 1"or ”SENS 2" LED. A digital readingof approximately .3.9.8 degrees (-13.98 volts at the OUTPUT screwterminals) will be shown. Note that the left, center and right decimalpoints are illuminated indicating an open circuit.

C. Remove the probe entirely andplace a jumper cable across the redand

yellow PROBE INPUTjacks to create a closed circuit. A digitalreading of approximately 2.7.9 degrees(+12.79 volts at theOUTPUT screw

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Section 5. SAFETY AND MAINTENANCE CHECKS

terminals) will be shown. Note that the center and right decimalpoints are illuminated indicating a closed circuit.

If your unitfails either step 3or 4, pleasecontact the BAS Service Coordinator to return both the LC-22Cand the probe used to make thesetests for service. Do not return any equipment without first obtaining a Return Authorization number from BAS.

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Section 6. SPECIFICATIONS

Temperature Setpoint

Temperature Measurement

Readout

Max. Load

Repeatability

Output TemperatureCalibration

0.00°C to 99.9°C in 0.1°C increments

-50°C to 115°C

Front panel LED

75 watts, eachchannel

0.1°C with thesame temperature probe.

Output is calibrated to100 mV per °C.

Rear Panel

Probe input jacks, outputtemperature jack, output power socket heaters,line voltage selector.

Heater Power

Heater power iscontrolled by zero crossing, solid state relays for noise-free operation.

Fuses

For 115V,2A Slo Blow, For 230V,1A Slo Blow