Baker SterilGARD SG303, SterilGARD SG303-INT, SterilGARD SG303-JPN, SterilGARD SG303-AUS Operator's Manual

Download

Revision

Date

Description

3/25/15

Updated branding.and split manuals

OPERATOR’S MANUAL

SterilGARD

Class II, Type A2

Biosafety Cabinet

MODEL

SG303, SG303-INT, SG303-JPN, SG303-AUS

This manual includes information for installation, operation, maintenance and spare parts. We recommend that it be kept near the cabinet for ready reference

INTRODUCTION AND WELCOME

It is a pleasure to welcome you to the growing number of customers who own and operate Baker biological safety cabinets. As the inventors of the laminar flow biological safety cabinet and the leaders in the field, Baker people take special pride in providing a cabinet that is designed for maximum performance.

Your new SterilGARDIII Advanceo cabinet includes many unique features which are included to give you superior performance, simpler maintenance and lower life cycle cost. Your SterilGARDIII Advanceo cabinet is designed for both safety and value.

In addition to the high quality you expect from all Baker equipment, this model has been ergonomically designed to provide the lab user with many exciting design features. The revolutionary ergonomic design will help prevent repetitive motion injury, reduce fatigue and lab accidents and enhance productivity.

You will find your SterilGARDIII Advanceo cabinet suitable for use not only for research and clinical diagnostic work involving tissue culturing of possibly infectious samples, but also for I.V. drug preparations and other pharmaceuticals that could have adverse health effects on operators and other techniques requiring a contamination-free atmosphere.

Please note that all open-front containment cabinets, including this one, are for use with low to moderate risk agents only. Open-front cabinets do not provide absolute protection for the user. The adequacy of a cabinet for user safety should be determined on-site by an industrial hygienist, safety officer or other qualified person. Remember that you, the owner and user, are ultimately responsible and that you use your cabinet at your own risk.

We recommend that this manual, along with factory test report, be kept near the cabinet for convenient reference by operators and qualified maintenance personnel. If you have any questions about the use or care of your new SterilGARDIII Advanceo cabinet, please do not hesitate to contact our Customer Service Department at 800-992-2537 for assistance or e-mail us at bakerco@bakerco.com.

Sincerely,

David Eagleson President The Baker Company, Inc.

P.O. Drawer E, Sanford, Maine 04073 (207) 324-8773 1-800-992-2537 FAX (207) 324-3869

Visit our website at www.bakerco.com

TABLE OF CONTENTS

I - FUNCTION AND DESCRIPTION OF THE STERILGARDIII ADVANCEO ................................ 3

AIRFLOW INSIDE THE BIOLOGICAL SAFETY CABINET ....................................................................... 3

Fig. 1 Airflow Inside the Cabinet ..................................................................................................................................... 3

POSITIVE AND NEGATIVE PRESSURE AREAS .......................................................................................... 4

ACCESS TO THE WORK AREA ....................................................................................................................... 4

DESIGN DETAILS ................................................................................................................................................ 4

Performance assurance ..................................................................................................................................................... 4

Ergonomic enhancements ................................................................................................................................................. 4

Motor/ blower capacity ..................................................................................................................................................... 5

One-piece interior wall construction ................................................................................................................................. 5

Protective screen ............................................................................................................................................................... 5

All-metal plenums............................................................................................................................................................. 5

Recessed stainless steel work surface ............................................................................................................................... 5

Cabinet exterior panels ..................................................................................................................................................... 5

Tested HEPA filters ................................................................................................................................ .......................... 5

Viewscreen ....................................................................................................................................................................... 5

Work area lighting ............................................................................................................................................................ 6

Drain Pan .......................................................................................................................................................................... 6

Air balance adjustments .................................................................................................................................................... 6

Easy filter access .............................................................................................................................................................. 6

Telescoping stand with adjustable feet. (optional)............................................................................................................ 6

Telescoping stand with castors. (optional)........................................................................................................................ 6

Solid-state electronic ballast ............................................................................................................................................. 7

Programmable Logic Controller ....................................................................................................................................... 7

UniPressure Plenum ......................................................................................................................................................... 7

Ground Fault Circuit Interrupter ................................................................ ................................ ....................................... 7

Ultraviolet Germicidal Lamp (optional) ........................................................................................................................... 8

SPECIFICATIONS ................................................................................................................................................ 8

Weight .............................................................................................................................................................................. 8

Electrical Specifications ................................................................................................................................................... 8

Environmental Conditions ................................................................................................................................................ 9

II - PREPARING THE STERILGARDIII ADVANCEO FOR USE........................................................ 9

CHECKING THE CABINET UPON ARRIVAL ............................................................................................... 9

THE USES OF A BIOLOGICAL SAFETY CABINET ..................................................................................... 9

LOCATION WITHIN THE LABORATORY .................................................................................................. 10

INSTALLING THE CABINET .......................................................................................................................... 10

CONNECTING THE EXHAUST ...................................................................................................................... 11

Exhaust into the room - ................................................................................................................................................... 11

Exhaust to the outside -................................................................................................................................................... 11

FINAL CONNECTIONS AND TESTS ............................................................................................................. 12

III PROPER CABINET USE ...................................................................................................................... 14

OPERATOR CONTROLS ................................................................... ERROR! BOOKMARK NOT DEFINED.

Figure 2 Operator Controls .............................................................................................. Error! Bookmark not defined.

START-UP PROCEDURE ................................................................................................................................. 15

WORKING IN THE CABINET ......................................................................................................................... 16

REACTING TO SPILLS ..................................................................................................................................... 17

DECONTAMINATION ...................................................................................................................................... 17

CLEANING AND DISINFECTING STAINLESS STEEL .............................................................................. 18

Simple Cleaning ............................................................................................................................................................. 18

Disinfection .................................................................................................................................................................... 19

USING ANCILLARY EQUIPMENT ................................................................................................................ 19

ABOUT THE HEPA FILTERS .......................................................................................................................... 19

REMINDERS ....................................................................................................................................................... 20

IV - ON-SITE CHECKS AND MAINTENANCE PROCEDURES ......................................................... 21

THE AIRFLOW BALANCE .............................................................................................................................. 21

FILTER LEAK TEST ......................................................................................................................................... 22

AIRFLOW SMOKE PATTERN TEST ............................................................................................................. 23

CABINET INTEGRITY TEST .......................................................................................................................... 23

ELECTRICAL SAFETY TESTS ....................................................................................................................... 24

MAINTENANCE NOTES ................................................................................................................................... 24

Cleaning the Work Area ................................................................................................................................................. 24

Ultraviolet Germicidal Lamp (optional) ......................................................................................................................... 24

Check the Mag Gauge Or Optional Air Flow Monitor(AFM) Regularly ....................................................................... 24

Replacing the HEPA Filters ............................................................................................................................................ 25

Figure 3 – Downflow Plenum ......................................................................................................................................... 26

TROUBLESHOOTING ...................................................................................................................................... 27

SG303 DISASSEMBLY INSTRUCTIONS ....................................................................................................... 30

APPENDIX .................................................................................................................................................... 32

SG303 DIMENSIONAL DRAWING ................................................................................................................. 33

SG303 LADDER SCHEMATIC ......................................................................................................................... 34

REPLACEMENT PARTS LIST ........................................................................................................................ 35

CHANNEL STAND INSTALLATION OR REMOVAL PROCEDURE ....................................................... 35

STAND ASSEMBLY/LEG EXTENSION PROCEDURE ............................................................................... 39

WARRANTY ................................................................................................................................................. 43

I - FUNCTION AND DESCRIPTION OF THE STERILGARDIII ADVANCEO

The SterilGARDIII Advance is a Class II Type A2 biological safety cabinet of original design. It features vertical laminar airflow and a front access opening. The unit is designed to protect not only the environment and the people using the cabinet, but also the product within from airborne contaminants.

Airflow Inside the Biological Safety Cabinet

The SterilGARDIII Advance cabinet features The Baker Company's momentum air curtain. See Figure 1. The stainless steel metal diffuser just below the supply HEPA filter creates a faster airflow behind the sash than over the work zone. The faster airflow in front makes an extremely effective air barrier.

Another feature of the unique Baker design is the high velocity return air slots, which maximize the cabinet's protective capability. It is generally accepted that maintaining containment and a particle free work area is most difficult in the area in which airflow turbulence is greatest — at the intersection of the side walls, the front access opening and the work surface. Turbulence caused by friction will also be found along a cabinet's side walls. In cabinets without high-velocity return air slots, this turbulence may also allow contaminants to escape from the work area, or it may make it possible for unfiltered room air to enter the work area.

The high-velocity return air slots are located along the side walls of the work area opening. Air is drawn into the slots at very high speed, preventing the escape of particulates and ensuring that no unfiltered air enters the work area. Additional high-velocity return air slots are located at the top of the sliding window to prevent gases, vapors or particulates from coming up behind the window and escaping into the laboratory. In the same way, they prevent room air from migrating down behind the window and contaminating the work area.

The airfoil design at the bottom of the sash opening has been designed to maximize containment while allowing comfort when performing procedures in the cabinet. The need for the airfoil in effective biological safety cabinet design has been documented in research conducted by Baker, the pioneers in safety cabinet development. Baker has continued this tradition with the SterilGARDIII Advance.

Fig. 1 Airflow Inside the Cabinet

Positive and Negative Pressure Areas

One of the many features that sets the SterilGARDIII Advance apart from other biosafety cabinets is the interaction of positive and negative pressure areas. The small positive pressure plenum is completely surrounded by a negative pressure area so that any particle passing through is bound to be drawn through the blower and trapped in a HEPA filter.

From the small positive pressure plenum, which is shown in Figure 1, a portion of the cabinet airflow is expelled through the exhaust HEPA filter. The remaining air is re-circulated through the supply HEPA filter where it re-enters the work area at better than Class 10 air. Because the cabinet must take in air to replace the air expelled through the exhaust filter, the same volume of room air enters the cabinet through the front access opening and the high velocity return air slots. This air, which does not reach the work area until passing through the supply HEPA filter, completes the air barrier at the front access opening.

All air is combined under the work surface. From here, the contaminated air is pulled, under negative pressure, through a plenum up the rear of the cabinet into the motor-blower, which blows it into a positive pressure plenum. Please note again that the positive pressure plenum is completely surrounded by negative pressure areas. If a leak should occur in a contaminated negative pressure plenum, such as the side wall or rear wall, the negative pressure will create suction and pull air in, not allowing it to escape into the operator's area. If there is a leak from the positive pressure plenum, the surrounding negative pressure area will recapture the contaminated air and re-circulate it through a HEPA filter.

Access to the Work Area

For easy entry of apparatus into the work area, SterilGARDIII Advance has a sliding viewscreen. Its ¼” safety plate glass allows excellent visibility and may be opened to a height of 18-1/4” to permit placement of items in the work area. The window should be set at a height of 8”. An alarm is provided to remind the operator if the window is above the maximum position for using the cabinet (see Section III, Proper Cabinet Use for how to use the viewscreen correctly while the cabinet is in use).

As with other Baker cabinets, the GFCI protected electrical outlets inside the work area are protected by a separate circuit breaker so that an electrical overload by ancillary equipment won’t affect air handling. Overloading with electrical equipment should, of course, be avoided in any case (see “Using Ancillary Equipment” in Section III, Proper Cabinet Use).

Design Details

Performance assurance

Meticulous care in manufacturing is followed by more than 14 separate performance tests prior to shipment of this cabinet. In addition, a complete factory test report on the performance of this cabinet is included with this manual.

Ergonomic enhancements

The Baker Company has also included ergonomic enhancements into the design of the SterilGARDIII Advance for improved operator comfort and safety. A 10-degree sloped front feature has been

incorporated into the design to improve ergonomics so the operator can use the cabinet properly without sacrificing comfort or safety.

Motor/ blower capacity

A motor/blower's capacity is measured by its ability to provide a nearly constant volume of air as resistance increases because of filter loading. Verification by a simulated filter-loading test has established that the SterilGARDIII cabinet is capable of automatically handling a 50% increase in pressure drop across the filter without reducing total air delivery more than 10%. With the use of the manual speed control, a 200% increase in the pressure drop across the filter can be handled. This results in extended filter life, which relates to significant cost savings over the life of the cabinet.

One-piece interior wall construction

The interior side and rear walls of the work area are constructed from a single piece of 16-gauge stainless steel. It has 7/16” [11.1mm] radius (rounded) corners to help prevent buildup of contaminants and aid in cleanup.

Protective screen

Located under the work surface at the bottom rear and sides of the return-air plenums, a protective screen, integral to the interior walls, is provided to prevent wipes and other paper materials from being drawn into the blower system. This feature eliminates costly decontamination processes and downtime. The screen affords easy access to trapped materials and should be kept clean at all times.

All-metal plenums

The plenums are constructed entirely of either carbon steel or stainless steel in order to provide strength, durability, air-tightness and resistance to deterioration.

Recessed stainless steel work surface

The work surface is constructed of corrosion-resistant 16-gauge type 304 stainless steel, with a satin finish that diminishes light reflection. It is recessed to retain spills, and the radiused corners make for easy cleaning and disinfection.

Cabinet exterior panels

External panel construction is of 16-gauge cold-rolled steel, or 16-guage stainless steel protected by a smooth white baked enamel finish.

Tested HEPA filters

Both the downflow and exhaust filters in the SterilGARDIII Advance are scan-tested HEPA filters. They are 99.99% effective on particles of 0.3 micron size. Each filter is scan-tested after installation to assure that there are no leaks >.01% of upstream concentration.

Viewscreen

The cabinet's ¼” safety plate glass sliding viewscreen may be opened to 18-¼ ” for placement of large items in the work area, and may be fully closed for system shutdown or UV light operation.

Stand w/ adjustable feet

Knee

Space

Height

Work

Surface

Height

Overall

Height

Minimum Setting

28 1/8”

30 1/8”

91 1/4”

Maximum Setting

36 5/8 ”

38 5/8 ”

3/4

”

Stand w/castors

Knee

Space

Height

Work

Surface

Height

Overall

Height

Minimum Setting

28 ”

30 ”

91 1/8”

Maximum Setting

34 ”

36 ”

97 1/8”

Work area lighting

The work area is illuminated by two external fluorescent lamps which provide a minimum of 100 footcandles of light at the work surface.

Drain Pan

The drain pan is designed with 7/16” radius in all four bottom corners to facilitate cleaning and disinfection. The work surface supports have been designed to be removable for easy cleaning and to allow clear unobstructed access to the whole drain pan area during the cleaning procedure. Drainage is provided by a 3/8” diameter stainless steel ball valve located at the bottom of the drain pan.

Air balance adjustments

Air balancing can be done by either of the following methods. However, it should be done only by a technician with proper training and equipment (see “The Airflow Balance in Section IV, On-Site Checks and Maintenance).

• A speed controller adjusts for minor voltage differences and filter loading.

• An adjustable external damper compensates for supply and intake imbalance due to pressure

resistance drop differences of supply and exhaust filters if they are changed at a later date.

Easy filter access

For convenience and ease of service, the downflow and exhaust HEPA filters are installed and removed through the front of the cabinet without entry into the work area. This is particularly helpful if the cabinet is connected to an exhaust duct. Filters should be replaced by qualified technicians only.

Telescoping stand with adjustable feet. (optional)

The optional support stand provides the following range of heights:

Telescoping stand with casters. (optional)

The optional support stand with casters provides the following range of heights:

Stands are shipped disassembled, providing a smaller package for shipping, handling and installation. The stand is easily assembled and attached on site to support the cabinet.

Note: instructions on assembling and installing the stand are packed in the cardboard box with the stand.

Solid-state electronic ballast

The SterilGARDIII Advance features solid-state electronic ballasts for fluorescent and optional UV light. These ballasts increase reliability, efficiency, service life and reduce heat.

Programmable Logic Controller

SterilGARDIII Advance is equipped with a programmable logic controller (PLC) enhancing reliability and diagnostics capability. The PLC is designed to have built-in immunity to momentary voltage interruption (flicker), electrical noise and vibration. Diagnostic indicators provide easier troubleshooting of critical components without the need of special instruments. A built-in RS232 communication port allows for interfacing to building management systems and provides flexibility for adapting options as they become available.

UniPressure Plenum

The telescoping all steel positive pressure plenum in the SterilGARDIII Advance provides the user with a number of desirable features which enhance safety and reliability while reducing down time during servicing:

1. This unique plenum design directs air across then through the HEPA downflow filter, improving

downflow uniformity, extending filter life and reducing noise.

2. The two piece plenum serves as the filter sealing mechanism providing a continuous and evenly

distributed clamping force around the complete perimeter of the filters. This approach eliminates potential filter frame damage as experienced with typical approaches, which utilize point forces. This method assures secure and sealed filters.

3. The filter plenum is mounted to the gasketed upstream side of the filter. The gasket on both upstream

and downstream filter frames reduces the transmission of vibration through the unit from the motor blower assembly.

4. The motor blower assembly is mounted on a slide plate, which forms one wall of the plenum. This

plate is removed by loosening one screw and disconnecting electrical plug connectors.

5. The two telescoping sections of the plenum are operated by turning two threaded rods in each

section. These screw clamps exert a force on the all-metal plenums, which distributes the clamping force uniformly to the filter frames.

Ground Fault Circuit Interrupter

The outlets on this unit are protected by a ground fault circuit interrupter (GFCI). The GFCI is designed to protect the operator from a possible electrical hazard. If the GFCI detects a hazardous condition, it will cut off electricity to all of the outlets. The button in the center of the left hand outlet will pop out. To reset the GFCI, correct the cause of the problem, then press the reset button on the GFCI.

Ultraviolet Germicidal Lamp (optional)

SterilGARDIII Advance can be equipped with an ultraviolet (UV) light. The light is controlled by an on/off switch on the front panel (see Figure 2, Operator Controls).

WARNING

• Do not defeat interlock, UV light is hazardous!

• Eyes and skin should not be exposed to direct ultraviolet light.

• Ultraviolet light should not be relied upon as the sole decontaminating agent. Additional surface

disinfection should be performed both before and after every cabinet use.

• A biological safety cabinet acts as a supplement to good aseptic practices, not as a replacement.

UV lamps lose their effectiveness over time and should be replaced when the intensity at the work surface drops below 40 microwatts per cm2 at a wavelength of 253.5 x 10-9 meters. Be sure to check regularly.

Specifications

Weight

The weight of the SterilGARDIII, Model SG303 cabinet is 397 pounds with a shipping weight of 534 pounds. With the optional stand, the cabinet weight is 457 pounds with a shipping weight of 594 pounds.

Electrical Specifications

115 VAC, 20A, 1 Phase, 60 Hz

SterilGARDIII Advance incorporates Baker’s StediVOLT motor speed controller for the blower motor. This compensates for minor variations in incoming line voltages.

SterilGARDIII Advance features one duplex GFCI protected receptacle protected by an internal 5A self resetting circuit breaker. The unit is furnished with one 16' power cord with terminated with a NEMA 5-20 plug. The power cord provides the cabinet and internal receptacles with a protective earth ground and serves as the main disconnect for the cabinet.

The unit is protected with a 20 amp, 250 VAC time delay fuse.

Blower motor (1,625 RPM) 3.20 amps Fluorescent Light (ballast) 0.49 amps Duplex outlets (G.F.C.I.) 5.0 amps Total running load 8.69 amps





Environmental Conditions

The SterilGARDIII Advance is designed to be used under the following conditions:

 Indoor use  Altitudes up to 2000 meters  Temperature range from 5C to 40C  Relative humidity up to 80%  Main supply voltage fluctuations not to exceed 10% of the nominal voltage  Maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative

humidity at 40°C

 Transient overvoltages according to Installation Categories (OVERVOLTAGE CATEGORIES) I, II

AND III (see Annex J). For mains supply the minimum and normal category is II

 POLLUTION DEGREE 2

II - PREPARING THE STERILGARDIII ADVANCEO FOR USE

Checking the Cabinet Upon Arrival

Upon receipt of your new SterilGARD Pay special attention to the red “Drop & Tell” modules indicating possible rough handling.

If there is any visible damage, that fact should be noted on the receiving slip and immediately reported to the delivering carrier.

Next, remove the packing material and inspect the cabinet itself. Remove it from the skid with a forklift or other available equipment. If any concealed damage is found it should be reported to the delivering carrier. A claim for restitution should be filed within 15 days.

Due to the risk of mishandling by trucking companies, The Baker Company has removed certain parts of the cabinet and packed them separately.

The Uses of a Biological Safety Cabinet

SterilGARDIII Advance has been designed to provide a work area which protects the experiment from the environment, and the environment and operator from the experiment. The laminar flow biosafety cabinet is designed for work with Biosafety Levels 1, 2 and 3 (low to moderate risk) agents as listed in The Center for Disease Control's "Biosafety in Microbiological and Biomedical Laboratories" U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and National Institutes of Health, U.S. Government Printing Office, Washington, D.C. 20402. (HHS publication number CDC 93-8395).

Biosafety Level 4 or extremely high risk agents should never be used in this cabinet, except in conjunction with a one-piece positive pressure personnel suit ventilated by a life support system within a high containment facility. Please consult the facility safety professional for a proper risk assessment.

III Advance

, first inspect the exterior of the crate, box and/or skid.

CAUTIONS

* The use of any hazardous material in the cabinet requires that it be monitored by an industrial

hygienist, safety officer or other qualified individual.

* Explosive or flammable substances should never be used in the cabinet unless a qualified safety

professional has evaluated the risk.

* If hazardous biological work is to be performed, apply the enclosed biohazard decal. This is in

accord with OSHA regulations (volume 39, number 125, Part II).

* If chemical, radiological or other non-microbiological hazards are present, be sure to employ

appropriate protective measures. Call upon a suitably trained individual to monitor the operation.

Location Within the Laboratory

The ideal location for any biological safety cabinet is in a dead-end corner of the laboratory away from personnel traffic, vents, doors, windows and any other sources of disruptive air currents. Published research from The Baker Company and unpublished tests performed at the National Cancer Institute show that if a draft or other disruptive air current were to exceed the intake velocity of the cabinet, contamination can enter the work area or escape from it (for more information, contact Baker). Proper placement within the laboratory is essential.

If the cabinet exhausts air into the laboratory instead of venting to the outside, it is important that there is adequate space between the top of the cabinet and the ceiling. A solid ceiling located too close to the exhaust filter will restrict the air and limit the intake velocity. The exhaust filter guard, an inverted “V” shaped perforated steel plate included with your cabinet, will provide the necessary distance between exhaust opening and ceiling. If the filter guard is not installed, the cabinet exhaust opening should be no closer than 2-¾” from the ceiling, to enable proper airflow. Consult with our Customer Service Department for the implications of this and for alternatives.

Installing the Cabinet

Installation of this cabinet should be carried out in accordance with appropriate OSHA regulations and those of other regulatory agencies having jurisdiction.

1. Remove the protective container from around the cabinet.

2. Remove the strapping that holds the cabinet to the pallet.

3. Remove the tape that secures the viewscreen, work surface and diffuser.

4. The small cardboard box taped to the work surface contains the drain valve and the exhaust filter guard.

5. If cabinet exhausts its air directly into the laboratory instead of ducting it to the outside, install the

exhaust filter guard on top of the exhaust damper using the two wing nuts installed on the damper.

6. The drain valve must be installed before the cabinet is either installed on the stand or placed on a counter

top.

MODEL

Sash Opening

Exhaust Volume (CFM)

Suction (“ W.C.)

SG303

8”

162

.02

Exhaust Connection Type

Gap

Exhaust flow rate (min/max)

(CFM)

Suction Min/Max (“ W.C.)

CEC (Canopy)

1”

260/370

.02/.08

7. If ordered with the optional stand, refer to the stand installation and assembly instructions packed in the

cardboard box with the stand.

NOTE: If you place your unit on the optional stand and you need to move the unit, we recommend you leave the unit on the stand. If you must take the unit off the stand to move it, disassemble the stand before moving it and reassemble at the new location.

Connecting the Exhaust

The SterilGARDIII cabinet can operate with filtered exhaust air entering directly into the room, or with filtered exhaust ducted to the outdoors. Details of these alternatives are as follows:

Exhaust into the room -

Never use the top of the cabinet or the work area for storage purposes, and never use flammable, explosive or toxic vapors or gases, or substances which generate them, unless a qualified safety professional has evaluated the risk. The filter removes only particulates and not gases, thus causing the recirculation of these within the cabinet.

Ensure that the exhaust filter guard is properly installed over the filter opening. The filter guard will provide the following advantages:

 Provide adequate space between filter opening and ceiling for proper airflow.  Prevent potentially damaging objects from being dropped onto the filter.

 Prevent objects from being placed over the exhaust opening and reducing the exhaust flow rate.

Exhaust to the outside -

Whenever possible, the filtered exhaust should be canopy connected to its own separate exhaust system. If it must be channeled into a ganged exhaust system, make sure that the system is not a recirculating type.

The exhaust requirements for a STERILGARD III Model 303 are as follows:

Note: Water column suction is measured directly above the cabinet exhaust damper before any dampers, elbows or other restrictions.

HEC (Hard)

N/A

162

.02

Note: With optional Air Tight Damper add .10 to the above suction requirements.

You may want to install an indicator light or some other safety device to give warning if the exhaust system fails. The optional exhaust transition seals around the exhaust damper and terminates in an 8” diameter by 2” high vertical collar. This design allows direct connection to standard duct work or canopy connector with an air gap. The transition also has an airtight damper to seal the cabinet for decontamination and an access panel for leak checking the filter and adjusting the balancing damper. The transition adds approximately 0.1” w.c. resistance to the system. For more details, please contact The Baker Company.

While the cabinet contains a damper to adjust for variations in filter resistance and sash opening size, the building exhaust system should contain provisions to adjust the building system flow and pressure.

Final Connections and Tests

1. The plumbing connection to the service petcocks must be made with great care because the effluent from

a safety cabinet may be biologically hazardous. When present, petcocks are piped within the cabinet. The external connection uses 3/8” compression fitting at the rear, top or bottom of the cabinet outer sidewall seal panel. Connection to plant utilities should be made per NFPA by qualified personnel with proper materials and technique. Flammable gas should not be piped into any cabinet. However, if the risk is professionally evaluated and a decision is made to install a flammable gas petcock, then an emergency shut-off valve should be situated in an accessible location outside the cabinet.

2. A 20-amp power cord with a NEMA 5-20P plug is furnished with the SterilGARDIII. It should be

plugged into an appropriate 115 Volt AC, 60Hz, 20 amp dedicated utility outlet.

3. Thoroughly clean the interior of the cabinet. Locate sash at correct access opening height 8”.

4. Press the blower switch. The yellow indicator below the switch should light.

5. Turn on the fluorescent light and make sure the bulb is lit. The lights will not operate unless the blower

switch is on.

6. If your cabinet has been purchased with the optional ultraviolet (UV) light, lower the viewscreen to its

fully-closed position and turn the UV light on to make sure it is operational. The UV light option features an interlock which prohibits its operation unless the viewscreen is completely closed and the fluorescent light is turned off.

7. Although all units are carefully tested at the factory, it is advisable that certain other checks are made on-

site by a qualified technician after installation. These include testing the filters for leaks and checking the air balance of the unit, especially if it is connected to an exhaust system. A description of these tests can be found in Section IV, On-site Checks and Maintenance.

8. It is also recommended that all personnel who will be using the cabinet study this Operator's Manual to

learn how to use the cabinet most effectively. For additional start up and use procedures, please turn to

Section III, Proper Cabinet Use.

FOR MORE INFORMATION

For a complete listing of articles, papers and reports related to containment, clean air products and safety, contact The Baker Company for our complete Bibliography or visit our website at www.bakerco.com.

ALARM

Alarm Mute / Reset

Sash Level Alarm

Blower

On / Off

Auxiliary Outlets

On / Off

Fluorescent Light

On / Off

UV Light

On / Off

Indicator

Lights

III PROPER CABINET USE

A biological safety cabinet is a valuable supplement to good sterile technique, but is not a replacement for it. If the cabinet is not understood and operated correctly, it will not provide an adequate protective barrier.

All activities that are to be performed in your cabinet should first be approved by a competent professional, such as an industrial hygienist or safety officer, to make sure that the cabinet is appropriate for the work it will be required to do. This person should monitor the cabinet and its operating personnel at regular intervals to see that it is being used correctly.

In order to keep the interior workspace clean and free of particulates, all Baker biosafety cabinets are designed for continuous operation. If the blower is turned off, the unit becomes contaminated with room air. Therefore it is recommended that the blower is left on at all times.

Operation and Controls

The operator controls with indicators are arranged on the front electrical panel of the cabinet. A number of switches are arranged in a single membrane switch assembly. [Reference Figure 3]

 Ultraviolet (UV) Light On/Off [Optional] – This switch controls the UV light inside the work area if

the UV light option is installed. The viewscreen must be fully closed before the UV light will turn on. The fluorescent light and the UV light are interlocked. When the viewscreen is closed, turning the UV light on will automatically turn the fluorescent light off. Turning the fluorescent light on will automatically turn the UV light off. The UV light will automatically shut off if the viewscreen is opened. A yellow indicator light located below the switch will illuminate when the switch is on.

Figure 2: Operator Controls

The yellow indicator light below the switch will light when the UV light is turned on. This unit is also capable of regulating the amount of time the UV light stays on which is determined by the operator. This function is very useful if the cabinet is to be left unattended for an extended period of time. To enable the timer, make sure the viewscreen is fully closed and the UV lamp is off. Depress the UV keypad switch and hold for 3 seconds. The alarm will pulse twice to signify that the timer mode has been entered. Release the switch. Immediately after, each press and release of the switch will program the timer to elapse one hour. (one pulse equals one hour, four pulses equals four hours, ect.) If no pulse is detected from the operator after four seconds, the timer begins to elapse automatically. The timer can be disabled at any time during or after the timing sequence by pressing the UV keypad or opening the viewscreen.

 Fluorescent Light On/Off – This switch controls operation of the fluorescent light. The cabinet

blower must be on for the fluorescent light to operate. A blue indicator light located below the

switch will illuminate when the switch is on.

 Duplex Outlets On/Off – This switch controls the duplex outlets in the work area. A blue indicator

light located below the switch will illuminate when the switch is on.

 Blower On/Off – This switch controls the power to the cabinet blower. A green indicator light

located below the switch will illuminate when the switch is on.

 Alarm mute/Sash level alarm – For normal operation, the viewscreen must be placed at the allowable

opening of 8” [203.2mm]. The sash alarm will be activated whenever the viewscreen is at a

potentially unsafe position. To mute the audible alarm, press the alarm mute button. The indicator light located below the switch will continue to flash. After five minutes, if the conditions persist, the alarm will sound again to remind you to reposition the viewscreen to its proper level. You may press the alarm reset switch again to mute the audible alarm for an additional five minutes.

Start-up Procedure

1. If the cabinet has not been left running continuously, turn on the blower. An indicator light located

below the switch will illuminate when the switch is on and the running blower will make an audible sound. Check the readings on the Magnehelic gauge, it should read a pressure consistent with the last time the cabinet was on.

2. Turn on the fluorescent light. The fluorescent light will not operate unless the UV light is turned off.

Never leave the UV light on while there is anyone in the room, unless viewscreen is fully closed.

3. Check to determine that the drain valve is in the closed position or the drain coupling is capped.

4. Wipe down the interior area of the cabinet with a surface disinfectant. Note: Some disinfectants,

such as bleach or iodine, may corrode or stain the steel surfaces. Good practice is to thoroughly clean the surfaces afterward with a detergent and rinse with sterile water to prevent corrosion.

5. Place all materials to be used for the next procedure inside the cabinet on the solid worksurface.

Disinfect the exterior of these materials prior to placing them on the worksurface. Everything required (and nothing more) should be placed in the cabinet before beginning work so that nothing

passes in or out through the air barrier, until the procedure is completed. Implements should be arranged in the cabinet's work area in logical order so that clean and dirty materials are segregated, preferably on opposite sides of the work area. Blocking the front and rear perforated grilles must be avoided. If wipes or absorbent towels are used on the work surface, be sure to keep them away from the grilles.

6. After equipment is in place inside the cabinet, adjust the sliding viewscreen so it is open no higher

than the correct access opening height ( 8”). An alarm will signal if that height is exceeded. This is important to maintain proper airflow.

7. After the cabinet has run for at least three minutes with the window in the proper position, it is ready

for use.

Working in the Cabinet

1. Hands and arms should be washed thoroughly with germicidal soap both before and after work in the

cabinet. Operators are encouraged to wear long-sleeved gowns or lab coats with tight-fitting cuffs and sterile gloves. This minimizes the shedding of skin flora into the work area and protects hands and arms from contamination.

2. Perform all work on the depressed area of the solid work surface. Work with a limited number of

slow movements. Since all of the equipment you need is already in the cabinet, it will not be necessary to move your arms in and out through the air barrier.

3. Because opening and closing doors in the laboratory causes air disturbance which might interfere

with cabinet airflow, this kind of activity should be kept to a minimum while the cabinet is in use. Personnel should also avoid walking by the front of the cabinet while it is in use.

4. Avoid using floor-type pipette discard canisters. It is important that your used pipettes be discarded

into a tray or other suitable container inside the cabinet. This reduces the temptation to move in and out of the work area unnecessarily.

5. Because of the restricted access, pipetting within the cabinet will require the use of pipetting aids.

6. Use good aseptic technique. Procedures done with good technique and proper cabinet methods will

not require the use of a flame. If, however, a safety officer approves the use of flame after evaluating the circumstances, then a burner with a pilot light such as the "Touch-O-Matic" should be used. Place it at the rear of the work area where the air turbulence caused by the flame will have the least possible effect. Flame disturbs the unidirectional airstream and also contributes to the heat load. If the cabinet blower is unintentionally turned off, the flame could also damage a filter.

7. Tubing for a burner within the cabinet should be resistant to cracking or puncture. Material such as

Tygon tubing is not acceptable for this use.

8. Never operate your cabinet while the viewscreen alarm indicator is on. The operating position of

the sash provides an 8” high access opening, depending on the design set at the factory. This restricted opening permits optimum operating conditions for the cabinet. Because operators will not all be the same height, it is suggested that the operator use a chair which may be adjusted for height.

9. After a procedure has been completed, all equipment which has been in contact with the research

agent should be enclosed, and the entire surface decontaminated. Trays of discarded pipettes and glassware should be covered. The cabinet should then be allowed to run for at least three minutes

with no activity so that the airborne contaminants will be purged from the work area. Next, make sure that all equipment is removed from the cabinet.

10. After you have removed all materials, culture apparatus, etc., decontamination of the interior

surfaces should be repeated. Check the work area carefully for spilled or splashed nutrient which might support bacterial growth. Never use the cabinet to store supplies or laboratory equipment.

11. We recommend that the cabinet be left running continuously to ensure containment and cleanliness.

If the user elects to turn the cabinet off at the end of a work session, the window should be closed completely. The sash alarm will be silenced when the window is in the closed position.

Reacting to Spills

Consult your safety professional for proper procedures and treatment of the specific agents you plan to use.

Decontamination

Whenever maintenance, service or repairs are needed in a contaminated area of your cabinet, the cabinet must first be decontaminated by an appropriate agent. The National Institute of Health, National Cancer Institute and the Centers for Disease Control have all recommended the use of formaldehyde gas for most microbiological agents. Its application requires individuals who are experienced in the decontamination of cabinets, since the gas itself is toxic.

A good reference for this procedure is NSF/ANSI Standard 49-2002 ANNEX G “Recommended

Microbiological decontamination Procedure”,NSF International, 789 North Dixboro Road, P.O. Box

130140, Ann Arbor, Michigan 48113-0140.

Whichever gas you choose, have the proper safety equipment (gas masks, protective clothing, etc.) within easy reach. In addition, you will want to be sure that the gas you are using will be effective against all of the biological agents within the cabinet. When you have decided which gas to use, post the antidote to it in a visible and nearby location. The volume of the SG303 cabinet is 30 cubic feet. Provide the correct amount of decontaminating gas for this volume.

Carcinogens and other toxins present a unique chemical deactivation problem and the standard biological decontamination will not, of course, be effective against chemicals or other non-biological materials. With materials of this kind, consult a qualified safety professional.

1. Surface-disinfect the inside of the window and all other surfaces on the view screen assembly.

2. Multiply the total volume of the cabinet (30 ft

paraformaldehyde required to decontaminate the cabinet. If the cabinet is vented to the outside you must consider the volume of the duct work in the paraformaldehyde calculation.

3. Prior to sealing up the cabinet make sure all gas or flammable petcocks are closed and pressure tight.

Use a soap bubble solution to make sure there is no leakage. Note: you are creating a confined space.

WARNING

by .3 gram/ft3 ) to determine the amount of

4. The inside cabinet work space should be at room temperature with 60% to 85% relative humidity. If

relative humidity is low (10 to 30%) add a pan of boiling water on the work surface. If it is (40% to 55%) add a pan of hot tap water on the work surface. Relatively humidity above 85% will require extra clean up which will be covered in Step 15.

Note: Without the proper relative humidity the formaldehyde gas will not be effective. The mode of entry of formaldehyde into the living organisms is through the cell wall by the absorption of water.

5. Place a heating mantle with paraformaldehyde in the work space. The heating mantle must be able to

reach 450°F and must have a grounded plug which should be plugged in to an outlet outside the cabinet.

6. This step is optional. Place a second heating mantle in the cabinet with 10% more by weight of

ammonium bicarbonate than paraformaldehyde. This will be used later in Step 13 to neutralize the formaldehyde gas.

7. This step is recommended. Place spore strips inside the cabinet to confirm the decontamination process

has been successful.

8. If the cabinet is vented to the room, use a sheet of plastic and seal the front access and exhaust port

openings. These openings should be sealed such that the exhaust airflow recirculates back to the front access opening. This will promote the even distribution of formaldehyde gas throughout the cabinet.

9. If the cabinet is vented to the outside, then the exhaust transition should have a means to recirculate the

exhaust airflow back to the cabinet blower. This will promote the even distribution of formaldehyde gas through the exhaust filter. Seal the front access opening.

10. Turn on the heating mantle containing the paraformaldehyde flakes.

11. After 25% of the paraformaldehyde has depolymerized, turn on the cabinet blower for 10 to 15 seconds.

Repeat after 50%, 75% and 100% of the paraformaldehyde has depolymerized.

12. Allow the formaldehyde gas a minimum residence time of 12 hours, preferably over night.

13. Turn on the heating mantle containing NH

HCO3 and the cabinet blower and allow the two gases to

circulate for at least one hour. Then vent the cabinet to the outside.

14. Aseptically remove spore strip and place in trypticase-soy broth and incubate for 7 days. No growth will

verify the decontamination process.

15. When cleaning up, you may find residual paraformaldehyde (white powder) on the metal or glass

surfaces. To remove this, use ammonia in warm water, wear gloves and wipe down the affected surfaces. The paraformaldehyde will dissolve in water and be neutralized by the ammonia.

Cleaning and Disinfecting Stainless Steel

Simple Cleaning

Do not use steel wool or steel pads when cleaning stainless steel.

Dirt deposits on stainless steel (dust, dirt and finger marks) can easily be removed. Frequently, warm water, with or without detergent, is sufficient. If this does not remove the deposits, mild, non-abrasive household cleaners can be used with warm water and bristle brushes, sponges or clean cloths.

Caution

Iron rust discoloration can be treated by rubbing the surface with a solution of 15% to 20% by volume of Nitric Acid and water and letting it stand for one to two minutes to loosen the rust.

Disinfection

The purpose of disinfection is to destroy particular organisms that could pose a potential hazard to humans or compromise the integrity of the experiment. It is important to use a suitable disinfectant in the concentration appropriate to the organism being killed. Standard disinfectants include: hypochloride (chlorine bleach), iodophor-detergent, ethanol and phenol.

Important

Disinfection and cleaning should always be followed by rinsing in clean hot water and wiping the surface completely dry.

Disinfect the work surface before and after every procedure.

1. Disinfect surfaces of all equipment used.

2. Remove all items from the inside of the cabinet.

3. Place all items that may have come in contact with the agent(s), such as used pipettes, in a biohazard

bag or other suitable container.

4. Disinfect the entire inside surface of the cabinet.

For additional information on cleaning and disinfecting stainless steel, please refer to:

“Decontamination, Sterilization, Disinfection, and Antisepsis”, Vesley, Donald and Lauer, James L.,

Laboratory Safety Principles and Practices, Second Edition, 1995, Fleming, D.O., Richardson, J.H., Tulis, J.J. and Vesley, D., editors, ASM Press, Washington, D.C., pp. 219-237; and Biosafety Reference Manual, Second Edition, 1995, Heinsohn, P.A., Jacobs, R.R. and Concoby, B.A., editors, AIHA Publications, pp.101-110.

Using Ancillary Equipment

The rule to keep in mind is that the more equipment placed in the cabinet, the greater will be the air turbulence it causes. The turbulence resulting from equipment and materials can disrupt the designed airflow and reduce the effectiveness of the cabinet. When you use equipment which rotates, vibrates or heats, be sure to place it at the rear of the work area if possible. This will minimize the turbulence at the access opening.

About the HEPA Filters

The High Efficiency Particulate Air (HEPA) filter is one of the essential components of a biosafety cabinet. It is the shield which stands between the operator and environment and the experimental agent.

The HEPA filter consists of a continuous sheet of glass fibers pleated over rigid corrugated separators and mounted in a rigid frame. It is very delicate and the filter media should never be touched.

Proven efficiency in all HEPA filters used in Baker cabinets is 99.99% for particles 0.3 microns in diameter. The 0.3 micron particle is used as the basis for filter definition because theoretical studies have shown that filtration efficiency should be at a minimum for particles of this diameter, with efficiency increasing for particles either larger or smaller. Experiments with various viruses and microbial agents have proven the effectiveness of HEPA filters. (Contact The Baker Company for more information)

It must be pointed out that the HEPA filter does not filter gasses or vapors, nor are they 100% efficient on particulates. Consider the filters’ inefficiency when performing a risk assessment. Since this cabinet is partially recirculating, there will be gaseous buildup to the point of equilibrium if these agents are used.

The life of a filter is determined by the cabinet motor/blower system design and how it is used and how often. Under normal laboratory conditions, you can expect at least five years of use. However, misuse or a heavy dust load within the cabinet will shorten any filter's lifetime. Bunsen burners and misuse of chemicals will also shorten the useful life.

Reminders

After many years of seeing our equipment used and misused, we have made up this list of suggestions:

 Store equipment and supplies outside of the cabinet.  Always leave the blower on.  Set the view screen at the proper height.  When possible, use pipetting aids.  Avoid use of an open flame within the cabinet unless the use has been specifically approved by a

safety professional.

 Always keep the air intake grills clear and unobstructed.  Only use toxic, explosive or flammable substances if a safety professional has approved them for

work in your cabinet.

 The control system will adjust automatically for small changes in the load on the blower.

Adjustments for larger changes should only be made by a qualified technician. Don't change the cabinet or blower speed unless the change is required by a decrease in measured air velocity.

 Work only with agents assigned to biosafety Levels 1, 2, or 3 in this cabinet.  Always close the drain valve after each use.  If the unit presents a warning signal, don't operate the unit until the warning ceases.

If the operators are well trained and use good common sense when operating your cabinet, you should have very few problems.

IV - ON-SITE CHECKS AND MAINTENANCE PROCEDURES

We recommend that the following checks be performed before initial use, after relocation and after each filter change. They should also be carried out at regular intervals, usually six months or one year, as specified by an industrial hygienist, safety officer or other qualified person. The tests described below meet recommended minimum requirements and only experienced technicians using proper procedures and instruments must perform them. Our representatives can tell you about other tests, which you may consider desirable.

As reported earlier in this manual, each individual cabinet made by The Baker Company is carefully tested before it leaves the factory. Your copy of the test report, which you will find at the back of this manual, gives the factory test results for your own SterilGARDIII cabinet. Use it as a record of the original testing, and as a guide to testing in the future. To gain many years of satisfactory service, please be sure that maintenance personnel come as close as possible to duplicating these original test figures.

Your test procedures should be identical to ours so that achieving similar test results and comparison of data will be possible. Please correspond directly with us to request detailed procedures for your particular cabinet model. Alternate testing procedures can be found in the NSF International Standard No. 49.

The Airflow Balance

Warning!

This procedure should be performed by qualified technicians only.

The airflow balance, which is set at the factory, provides your cabinet with the proper air volume and velocity control to minimize leakage of airborne contamination either into or out of the work area.

In order to duplicate as closely as possible the airflow characteristics described in the original factory test report, please follow these steps:

1. Adjust the window to its designed opening height. If the window is set above this height an alarm will

sound.

2. Perform inflow velocity test per NSF 49. Specific details are on the cabinet instruction label and in the

factory test report.

NOTE: the IV bar and UV light bulb need to be removed while performing downflow readings.

3. Perform downflow velocity test per NSF 49. Specific details are on the cabinet instruction label and in

the factory test report.

4. Compare these results with those originally recorded at the factory.

Warning!

Condition

Adjustment

Downflow

Inflow

Damper

Speed Controller

High

High Decrease

Low

Low Increase

Low

High

Close High

Low

Open OK

Low

Open

Increase

High

Decrease

Low

Increase

High

Open

Decrease

When the light canopy is lowered, some electrical parts are exposed. Do not perform any work inside the canopy unless you are a trained electrician or electronic technician.

5. Make adjustments to the blower speed controller and the damper as necessary. The speed controller is

located inside the light canopy. Turn the screw clockwise/counterclockwise to increase/decrease flow. The damper is located on top of the cabinet. Slide the damper open/closed to increase/decrease flow. The chart below may aid with making the correct adjustment:

NOTE: as the HEPA filters load up with particulates, airflow will be maintained automatically, at least until the filter resistance increases 50% or more. When airflow eventually diminishes, you will have to increase the blower speed in order to maintain the original volume of recirculating air. There is a speed control located in the light canopy. Turn it clockwise until you have the desired airflow. If the airflow cannot be maintained, it will be necessary to replace the HEPA filters. (See “Replacing the HEPA filters” later in this section).

Filter Leak Test

The filters in your cabinet were tested at our factory before shipment to ensure that the media, gasket, and frame were not exceeding NSF International Standard 49 allowable leak rates. Since filters may become damaged in transport, we recommend that the filters be re-tested by qualified technicians before the cabinet is used. The filters should also be leak tested at prescribed intervals as specified by an industrial hygienist, safety officer or other qualified person. NSF International details the steps for performing the filter leak test in their Standard 49.

Filter leak test procedures:

1. Remove diffuser under downflow filter and external damper over the exhaust filter to access filter media

and frame.

2. Perform calculations/measurements for upstream concentration of aerosol challenge.

Warning!

Decontaminate the unit before measuring the upstream concentration of aerosol in a contaminated plenum.

3. Perform filter leak test per NSF 49.

4. Repair leaks as required.

Airflow Smoke Pattern Test

We recommend that qualified technicians verify the direction of airflow within your cabinet before the cabinet is used. The direction of airflow should also be verified at prescribed intervals as specified by an industrial hygienist, safety officer or other qualified person. NSF International details the steps for performing the airflow smoke pattern test in their Standard 49.

Cabinet Integrity Test

The sealing integrity of your cabinet was tested at our factory before shipment to ensure that the welds, gasketed seams and hardware were free of detectable leaks. Since these items may become damaged in transport, we recommend that they be re-tested, by qualified technicians, before the cabinet is used. The cabinet integrity test should also be performed at installation, relocation and at prescribed intervals as specified by an industrial hygienist, safety officer or other qualified person. NSF International details the steps for performing the cabinet integrity test in their Standard 49.

Cabinet Integrity Test Procedure:

1. Decontaminate the cabinet if it has been used.

2. Disconnect cabinet from electrical source.

3. Shut off all gas, air and/or vacuum ports.

4. Seal off the exhaust opening by taping around the external damper and the slot, or by taping a piece of

cardboard or plastic over the opening. If the cabinet is connected to an external exhaust, close the gas tight damper or remove ductwork to allow sealing.

5. Remove the light canopy, the electrical panel and the window tracks.

6. Seal the front window opening by taping a piece of heavy gauge plastic around the perimeter of the

opening.

Decontaminate the unit before pressurizing, if it has contaminated plenums.

7. Perform cabinet integrity test per NSF 49.

8. Repair leaks, as necessary.

9. Restore the cabinet to operating status. Be sure to remove the sealing material, clean the cabinet and

reassemble all components.

Warning!

Electrical Safety Tests

The electrical leakage, ground circuit resistance and polarity were tested at our factory before shipment to ensure that there is no risk of electrical shock present in your cabinet. Since electrical components may become damaged in transport, we recommend qualified technicians retest them, before the cabinet is used. The electrical safety tests should also be performed at prescribed intervals as specified by an industrial hygienist, safety officer or other qualified person. NSF International details the steps for performing these tests in their Standard 49.

Note: while performing the electrical safety tests, ensure that the connections with the test leads are solid, as poor connections will increase the resistance reading. Also, ensure that the exposed metal being touched is solidly connected to the cabinet frame. Gasketed panels may not provide a reliable measurement.

Maintenance Notes

Cleaning the Work Area

Spills that fall through the perforated grills can be removed through the drain valve after proper decontamination.

To wash the drain pan under the work surface, lift up and surface decontaminate the solid work surface, the perforated grill and remove the work surface supports. This provides unobstructed access to the drain pan for easy cleaning. Remember that this area must be assumed to have contamination, so use caution in the way you approach the task. Make sure you close the drain valve when you are finished cleaning the drain pan.

Ultraviolet Germicidal Lamp (optional)

As reported in other sections of this manual, UV germicidal lamps lose their effectiveness over time and should be replaced when their intensity at the work surface drops below 40 microwatts per square centimeter at a wavelength of 253.5 x 10-9 meters.

If your cabinet has a germicidal lamp, frequently measure its intensity at the geometric center of the work surface with an ultraviolet light meter. The appearance does not indicate UV effectiveness.

Check the Mag Gauge Or Optional Air Flow Monitor(AFM) Regularly

Changes in areas surrounding the laboratory may produce unexpected dust or other conditions which affect the filters. To maintain filter integrity and good cabinet operation, check the mag gauge or AFM periodically.

Replacing the HEPA Filters

When the certifier can no longer set the cabinet air flows to within 5 FPM of the nominal set point by adjusting the speed control or the damper, the filters must be replaced. If the filters are damaged, they will also need to be replaced.

Before any seal panels are removed, the cabinet should be decontaminated. Please see “Decontamination Procedure” in Section III Proper Cabinet Use for specifics on decontamination. The filters are sure to have collected microorganisms and other potentially harmful particles generated in the work area during their lifetime, and maintenance personnel should not allow themselves to be exposed. It should also be remembered that a specific gaseous decontamination may work against microorganisms, but not against chemical agents. Where chemicals are present, consult an industrial hygienist or other qualified person.

A chemically contaminated filter must be handled with caution. Clothing and/or breathing apparatus should be used to protect personnel as necessary to reduce the hazard. It is advisable to seal the contaminated side of the filter by taping a plastic sheet or cardboard over the face before removal. This should minimize the number of particles shaken loose from the filter. Once removed, the filter should immediately be sealed in a chemical hazard bag and then disposed of safely in accordance with environmental regulations.

After filter replacement has been completed, the cabinet and the room should be cleaned and decontaminated in a manner consistent with the nature of the hazardous material. The cleaning materials, along with the protective gear and clothing, should be properly disposed.

HEPA filters are very easily damaged, and you will want to use great care in handling so as to avoid injury to the filter media and gasket surfaces. When installing the new filters, it is a good idea to tape a piece of cardboard over the filter media to give protection against dropped wrenches or misdirected fingers. Of course, you'll need to make sure that the cardboard is removed before the access panels are re-installed. Inspect the filters carefully before and after installation. A broken or damaged filter is worthless.

For detailed instructions on changing the filters, see the following two sections.

Changing Filters

Decontaminate the unit before changing filters.

1. Lower the sash to the fully closed position.

2. Disconnect the tygon tube from the back of the mag gauge and lift off the outer closure panel.

3. Remove the canopy locking fasteners inside the inner closure panel and then lower the canopy.

4. Remove the front dress panel by removing the two bolts at the top and loosening the two bolts at the

bottom.

Warning!

5. Loosen each of the eight 3/8” hex head bolts on the front seal panel. Be sure not to damage or lose

the seal washers on each bolt.

6. Remove the front seal panel making sure that you do not damage the gasket. The filters are now

exposed.

7. Loosen the ½ - 13-plenum clamp bolts, located on each side of the downflow plenum, approximately

3 ½ revolutions or until the supply plenum is raised about ¼” above the filter. See Figure 3.

8. Loosen the filter gasket seal from the metal plenum. A putty knife works fine to break the gasket seal

if necessary. Align the downflow filter with the notches in the frame of the plenum and remove the filter by sliding it towards you.

NOTE: once removed, the filters should immediately be sealed in a chemical hazard bag appropriately marked for the type of hazard and then disposed of safely in accordance with environmental regulations. If you are not removing the exhaust filter, proceed to step number 11. If only the exhaust filter is to be changed, the downflow plenum clamp bolts (lower) should be loosened four or more revolutions before loosening the exhaust plenum clamp bolts (upper).

9. Alternately loosen the ½ - 13-plenum clamp bolt, located on each side of the exhaust plenum,

approximately 3 ½ revolutions or until the exhaust plenum is lowered about ¼”. See Figure 3.

10. Loosen the exhaust filter gasket seal from the metal plenum. Remove the exhaust filter by sliding it

towards you.

Figure 3 – Downflow Plenum

11. Replace the filter(s) by simply aligning them with the notches in the frame of the plenum

and sliding them back against stops in rear, ensuring that the gasket remains attached on both sides.

12. The exhaust plenum clamps should be tightened first, alternately two to three revolutions

at a time until the filter gasket is compressed to approximately 1/8”.

13. The downflow plenum clamps can now be tightened, alternately two the three revolutions

until the gasket is compressed to approximately 3/16”.

14. Reinstall the front seal panel by first tightening each ¼-20 hex cap screw until the bolt

seal washer is engaged with the panel. Next turn each bolt 1¼ to 2½ revolutions until the panel gasket is evenly compressed to 1/8” thickness. Do not overtighten. (Do not use a

power drive socket for this procedure)

Troubleshooting

Here are some suggestions based on our experience with the use and misuse of biological safety cabinets.

When a smoke test indicates that there is air flowing from the interior of your cabinet into the surrounding room –

 Make sure that the exhaust damper is adjusted to its factory-marked position and that no

other objects are blocking the airflow.

 Make sure that the stainless steel downflow diffuser is installed in the work area under

the downflow HEPA filter.

 If your cabinet is connected to an in-house exhaust, make sure that there is adequate

exhaust suction and the system is not producing back pressure. Also, be sure the dampers are open. Re-balance the exhaust system to handle an adequate volume of air and static pressure (suction). Consult with building maintenance people.

 The exhaust filter may be loaded with dirt if the unit has been in service for some time.

Decontaminate, and replace all HEPA filters.

 There may be high cross-drafts in the room which are causing the outflow of smoke.

Check the airflow balance, following the procedure recommended in an earlier section of this Section. Eliminate the source of the cross draft.

When there is low airflow within the work area and through the exhaust filter -

 Check the incoming line voltage. Low voltage may cause the blower to operate at a

slower-than-designed speed. Although this should be corrected in the building's electrical system, you may be able to compensate by adjusting the blower speed control clockwise until proper velocity is reached. The control is located on the left side of the light canopy.

 Check the filters for damage. Decontaminate the unit and replace the filters, if necessary.

If there is no air flow within the work area -

 Check to make sure the blower switch is turned on. The yellow indicator below the button

should be lighted.

 Check to make sure the unit is plugged into a dedicated electrical outlet (grounded 20

amp, 115 Volt AC, 60Hz).

 Check to make sure the wiring connections inside the left end of the light canopy are

pushed together properly.

 Check to see if the lights are working. If they are, then turn the blower switch off and let

the cabinet set for ten minutes. This allows the motor time to reset itself, if it has been overheated.

Note: if the motor restarts and there is a whining sound, the motor bearings may be causing the motor to overheat.

 If these solutions do not correct the problem, or if the blower failed to start after the rest

period, then the speed control, blower motor or capacitor is defective. A qualified electrician, using the wiring diagram in the Appendix of this manual, can by-pass the speed control to determine if it is defective.

If one (or both) of the electrical outlets does not function -

 Check to make sure the outlet switch is in the on position. The yellow indicator below the

switch should be on.

 Check the reset button on the GFCI outlet. If the GFCI has tripped out, press the reset

button.

 The outlets are also protected by a self-resetting thermal circuit breaker. A qualified

electrician, using the wiring diagram in the Appendix of this manual, can check this breaker.

If the ultraviolet light does not work -

 Ensure that the window is completely closed and the fluorescent light is off.  Check for proper electrical connections between the ultraviolet bulb and lamp sockets.  Check the ultraviolet light bulb; replace if necessary.  Have a qualified electrician check the wiring and ballast for continuity. The wiring can be

traced to the source of a break. If none of the above is effective, the ballast may need replacing.

When there is uneven fan operation, or noise from the motor/blower assembly -

NOTE: it may be necessary to decontaminate the cabinet.

 Check the speed controller.  Lower the light canopy from the unit and check the multi-pin connectors at each end to be

sure they are securely engaged.

 Check the blower wheel for contact with the blower housing.  Check the blower for loose objects.

When the window open alarm is sounding -

 Check to ensure the window is at the correct position. The alarm should be silent when

the window is at the proper design opening. The indicator below the alarm-reset button will flash. You may cancel the audible alarm for five minutes by pressing the alarm-reset

button. The indicator will continue to flash until the window is set in the correct position. After 5 minutes, the alarm will sound again. You may press the alarm-reset button again.

If the fluorescent light does not work -

 The blower switch should be turned on and the yellow indicator below the switch should

be lit.

 Lower the light canopy from the unit and check the multi-pin connectors at each end to be

sure they are securely engaged. If the lamp flickers and can be corrected by vigorous rubbing of the bulb, there is probably an improper ground. The wiring can be traced to the source of a break.

If the air magnehelic gauge has high or low readings –

A higher reading than 0.15 to 0.17 inches water column could be an indication of the following:

 Blocked or partially obstructed perforated grills (front and/or rear).  Towels or wipes have blocked the towel guard on either the back or sides of the unit.  The viewscreen is in the closed position and the in-house exhaust fan is still pulling air

through the cabinet.

 Incorrect air flows

For any of the above conditions, always check for proper air flows first.

A lower reading than 0.15 to 0.17 inches water column could be an indication of the following:

 Partially or totally blocked filters.  Incorrect air flows.  Perforated grills or worksurface has been removed.

For any of the above conditions, always check for proper air flows first

Sg303 Disassembly Instructions

NOTES: Refer to Figure 3 – Downflow Plenum for blower, filter and plenum configuration.

For Motor/Blower removal, follow steps 1-18. For filter removal, follow steps 1-15 & 21-23.

TOOLS REQUIRED: (1) 3/8” spin wrench, (2) 3/8” socket and drill, (3) ¾” wrench and (4) ¼”

spin wrench

DISASSEMBLY:

1. Remove front cover. a. Lift out at the bottom enough to disconnect the magnehelic gauge hose. b. Lift out at the bottom and carefully lift up and away from the unit.

2. Remove the thumb screws that retain the face frame to the canopy (located inside of

the front cover frame).

NOTE: lower the sash to the closed position.

3. Drop the canopy down on its restraint cables and disconnect the limit switch wires.

4. Disconnect the canopy wires from the circuit board on the right hand side of the

canopy.

5. Remove cable from relay.

6. Disconnect the restraint cables from the face of the unit and lift off the canopy at the

bottom.

7. Remove the two retaining bolts at the top of the unit that secure the front dress panel.

8. Loosen the four retaining bolts on the outer edges of the electrical board panel that

houses the electrical components.

9. Disconnect the cord assembly at the top of the unit that goes up from the electrical

board assembly.

10. Disconnect plug on left side.

11. Lift off the whole electrical board assembly with the cord assembly attached, and

store in a safe place.

12. Remove front dress panel.

13. Remove the window by lifting up and out. Store in a safe place.

14. Using a 3/8” drive socket and drill, remove eight retaining bolts from the front access

panel. Remove the center top bolt last.

15. Loosen the track retaining bolts and remove the tracks by sliding out horizontally.

16. Blower/motor removal - remove one bolt, lock-washer and clip at the forward edge of

the positive pressure plenum.

17. Disconnect the motor leads by pulling apart at each connection.

18. Remove the blower by sliding it out the front.

19. Raise the positive pressure plenum approximately ¼” so that the filter can slide out.

20. Remove the downflow HEPA filter by sliding out of the front.

21. Loosen the exhaust filter plenum by adjusting the two exhaust plenum adjusting bolt

assemblies.

NOTE: pull towards you on the left-hand. retaining bolt and away from you on the right-hand. retaining bolt.

22. Lower the exhaust filter until it clears the front top edge of the unit.

NOTE: break the exhaust HEPA seal by lifting up gently at both front corners.

23. Pull exhaust filter straight out towards you.

24. Remove the side panels - (7) retaining bolts per side.

25. Loosen the bolts on the inside of the exhaust plenum enough to allow the clamping

hardware to angle out away from the slotted retaining brackets.

26. Remove the ¾” nuts from each petcock plumbing connection.

27. Repeat the process on the other side.

28. Lift out the front perforated grill at the worksurface.

29. Lift out the worksurface pan.

REASSEMBLY: Use the disassembly instructions in reverse order.

APPENDIX

17 11/16

WORK

SURFACE DEPTH

5. CABINET PLUMBING CONNECTORS ARE 3/8 PARKER FITTINGS. 3/8 NPT ADAPTER

4. PETCOCKS RECOMMENDED FOR USE WITH NONFLAMMABLE GAS, AIR, OR VACUUM

3. FILTER, MOTOR, BLOWER AND ALL ELECTRICAL COMPONENTS ARE FRONT ACCESSIBLE.

ACCESS PANELS, BLOWER SECTION: 16 & 18GA. C.R.S. BAKED ENAMEL FINISH, BAKER PERMA WHITE

SHIPPED WITH CABINET.

AT 30 P.S.I. MAXIMUM OPERATING PRESSURE.

2. CONSTRUCTION:

WORKSURFACE/WORKAREA: 16GA. 304 SS, #4 FINISH

NOTES:

SHOULD NATURAL GAS BE USED, PLEASE ADVISE OF ANY SPECIAL PLUMBING REQUIRED

BY YOUR LOCAL PLUMBING CODES; (ie BLACK IRON)

1. ELECTRICAL REQUIREMENTS: 115 VAC, 60 Hz, SINGLE PHASE, 1

Ø 20 AMP

30 9/16

NOTE: With optional Air tight damper add .10 to the

above pressure loss.

EXHAUST REQUIREMENTS FOR SG303

WITH OR WITHOUT EXHAUST TRANSITION

95 7/8

99 3/4

63 1/8

CABINET

CONTROLS

27 3/4

WORK

SPACE LENGTH

GFI PROTECTED RECEPTACLE

5 AMP TOTAL

LOCATED CENTER OF BACKWALL

4 1/2

C.L.

OF VALVE

3 1/4

BALL VALVE

DRAIN, 1/2" NPT

MAX. HEIGHT

87 3/8"

MIN. HEIGHT

ONE PLUGGED

PENETRATION

MAX. HEIGHT

91 1/4"

MIN. HEIGHT

36 5/8

MAX

28 1/8" MIN.

HEIGHT TO

BOTTOM OF UNIT

(INCLUDES 2 1/2"

OF LEG LEVELER

ADJUSTMENT)

27 1/4

WORK

SPACE HEIGHT

ONE PETCOCK

(SEE ITEM 4 IN NOTES)

BOTTOM

OF DRAIN PAN

61 1/8"

TO BOTTOM

OF WORKSURFACE

17 3/4

MAX. OPENING

8.0

(STANDARD)

SASH HEIGHT

1 1/8

2.0

TO BOTTOM

OF WORKSURFACE

35 5/8

OVERALL LENGTH

MAG GAUGE

DOP PORT

ACCESS REQUIRED FOR

CABINET CERTIFICATION

SASH HEIGHT

EXHAUST FLOW RATE

162 CFM

±5

EXHAUST DAMPER

ACCESS REQUIRED FOR

CABINET CERTIFICATION

DETAIL B

SQUARE FOOTAGE

STERILGARD, MODEL SG303

TMartin

8/19/2002

±.06

±.03

±.010

NO 4

SHEET METAL

DRAWN

AJohnson

APPROVED

CHECKED

FRACTIONS

FINISH:.X.XX

.XXX

DECIMALS

±1/32

125

±.005

MACHINED

±.01

±.02

TITLE

SIZE

DATE

3/1/2002

DATE

SCALE

ANGULAR

±0'-30° (.5°)

THIRD ANGLE

PROJECTION

MATERIAL

3/8" BULKHEAD FITTING

(TYP OTHER SIDE)

1/2

1 3/16

PLUG

DETAIL C

1 OF 1

SHEET

BGA-SG303-A2-1

DWG NO

REV

6 3/8

REV

DESCRIPTION

DATE

DRN

APP-BY

APP-DATE

ORIGINAL RELEASE

NEMA

5-20 AMP

POWER CORD WITH

20 AMP PLUG 14 FT.

EXTERNAL LENGTH

PRESSURE LOSS

0.02" W.C.

OPTIONAL STAND

SG303 Dimensional Drawing

SG303 Ladder Schematic

Part Description Part # Capacitor 11557

Circuit Breaker 34331 Exhaust Hepa Filter 39447 Fluorescent Ballast 40093 Fluorescent Lamp 38684 Front Panel 332P605 Ground Fault Interrupter Outlet with Indicator Light 34921 Lamp Holder Base 35072 Lamp Holder Clip 35071 Limit Switch 16651 Mag Gauge 20773 Interface Board 36504 Motor/Blower Controller 304227 Relay, Solid State 39636 Sash Balance 39554 Side Panel, LH 332X602 Side Panel, RH 332X601 Standard Outlet 18231 Supply Blower 11417 Supply Diffuser 332P105 Supply Hepa Filter 39861 Supply Motor 39799 Switch, Touch Type 37919 Power Supply 39404 Micro-Controller 39407 Ultraviolet Lamp 18022 Ultraviolet Lamp Ballast 31354 Ultraviolet Lamp Socket 18088 Viewscreen Assembly 332A300 Viewscreen Frame Assembly 332A301

REPLACEMENT PARTS LIST

Item Number

Description

Qty

Channel stand, back

Channel stand, side

Bolt, carriage,¼” x 1-1/4” long

Bolt, hex head, 3/8” x 1” long

Flat washer, ¼”

Lock washer, ¼”

Flat washer, 3/8”

Lock washer, 3/8”

Hex nut, ¼”

Adjustable leg leveler (or optional castor)

Step 1: Gather all stand components, hardware, and tools.

Step 2: Place stand sides on a flat

floor so that the stainless steel legs are setting on the floor. Next place the stand back between the sides so that the open part of the channel is facing towards the floor.

Step 3: Using the 3/

”x 1” hex

head bolt with lock and flat washers [Items 4, 7 & 8], attach the sides and back by inserting the bolts through the (4) holes located in the channel stand back into the already installed rivnuts in the two sides. (2) bolts per side. Do not tighten any of these bolts until you have all (4) threaded in slightly. Tighten bolts after this is done.

Channel stand, back

Channel stand, side

Channel stand, back

/8” Hardware Flip the stand 90º to access lower hole

Channel Stand Installation or Removal Procedure

Tools required: 7/

Parts List:

” deep socket,

/16” wrench or socket

Note: to install a stand please follow Steps 1-7. To remove a stand, please reverse Steps 1-7.

Step 4: With stand assembly complete, rotate stand upside down. Attach the leg levelers by screwing them into the bottom of the telescoping legs.

The telescoping legs can now be adjusted to the desired height requirement. This is done by removing the 3/8" bolts in each leg, extending the leg and reinstalling the bolts at the new hole location (2 bolts per leg).

NOTE: If the cabinet is not in its final room location you may want to perform the leg adjustment after assembling the cabinet to the stand and moving it to the room.

Step 5: Rotate the stand assembly to the upright position so that the leg levelers are now resting on the floor.

Step 6: Lift the cabinet high enough to allow clearance for the stand assembly to be positioned under it.

Once the stand is positioned under the cabinet, install (4) ¼” x 1-1/4" carriage bolts into the key way slots located under the cabinet in each of the (4) corners. The cabinet is now ready to be lowered onto the stand. There are (4) holes on the top of the stand which the bolts must be guided through. Be careful

Telescoping leg

Leg leveler

/8” Hardware [Items 4, 7 & 8]

¼” x 1-1/4” carriage bolt

Channel stand

Cabinet

not to let the bolts drag on the edge of the holes while lowering as burrs may occur on the bolts and cause threading problems.

Step 7: With the cabinet resting on top of the stand assembly, place (1)

¼” flat washer, lock washer, and

hex nut onto each of the bolts. Tighten after all (4) nuts are threaded on.

Step 8: The lift device should be removed if not being used to move cabinet into position within the room. If the lift device is mobile it may be used to maneuver cabinet into the desired location. A blanket could also be placed under the leg levelers while sliding the cabinet to avoid scuffing the flooring.

¼” Hardware

Item No.

Description

Qty.

Adjustable Foot or Caster Option

3/8”-16 x 1” Hex head bolt

3/8” Lock washer

3/8” Flat washer

7/16” Plug button

2 3 4 5 1

Stand Assembly/Leg Extension Procedure

The cabinet is shipped with the legs bolted in the shipping position and has two work-surface height settings per option:

 30-1/8” and 38-5/8” (with the adjustable foot option)  30” and 36” (with the caster option)

Remove the following parts from the hardware box shipped with the unit. Check the quantities with the list below.

Slide the unit off the pallet

1. To protect the floor finish, use a moving blanket. Carefully slide the unit off the pallet onto the

moving blanket.

2. Slide the unit into its destination room before going further with the assembly/installation.

Installing the Adjustable Foot or Caster Option

3. Lift one end of the cabinet/stand assembly up a minimum of 7” and block in position. See

illustration below.

4. Screw in two of the adjustable feet or casters (item # 1). Screw item #1 in by hand as tight as you can

and remove the blocks.

5. If your cabinet was purchased with the caster option be sure to chock the previously installed

casters to avoid cabinet movement, then repeat steps 3 and 4 for the other end of the cabinet.

6. Only the adjustable foot option is shown above for clarity.

Raise the Unit to 30 1/8” work-surface height (adjustable foot option) Raise the Unit to 30” work-surface height (caster option)

7. Lift one end of the unit up a minimum of 5” and block in position.

8. Unscrew the bolts (one per leg) that hold the legs in the shipping position.

9. Slide the leg out of the stand until the holes line up at the next position.

10. Bolt the leg in position with items 2, 3 and 4 (two bolts per leg).

11. Repeat steps 8 and 9 for the other leg and remove blocks.

12. Repeat steps 6 through 10 for the other end of the unit.

13. Plug open holes using item 5 (one per leg).

To raise the unit to the 38 5/8” work-surface height (adjustable foot option) To raise the unit to the 36” work-surface height (caster option)

14. Lift one end of the cabinet up a minimum of 7” and block in position.

15. Unscrew the bolts (two per leg) that hold the legs in the current work-surface-height position.

16. Slide the leg out of the stand until the holes line up at the next position.

17. Bolt the leg in position with items 2, 3 and 4 (two bolts per leg).

18. Repeat steps 15 and 16 for the other leg and remove blocks.

19. Repeat steps 13 through 17 for the other end of the unit.

20. Plug open holes using item 5 (two per leg adjustable foot option) (one per leg caster option)

Turn in opposing directions for fine adjustment to aid in leveling of cabinet.

Warranty

The Baker Company, Inc., expressly represents and warrants all goods (a) to be as specified (and described) in The Baker Company catalogues and literature, and (b) to be free under normal use, service and testing (all as described in The Baker Company, Inc., catalogues and literature) from defects in material and workmanship from a period of thirty-six (36) months from the invoice date [US/Canada only] and Twelve (12) month warranty for international.

The exclusive remedy for any breach or violation of this warranty is as follows: The Baker Company, Inc., will F.O.B. Sanford, Maine, furnish without charge repairs to or replacement parts or equipment which proved defective in material or workmanship. No claim may be made for any incidental or consequential damages.

THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTIBILITY OR FITNESS FOR A PARTICULAR PURPOSE UNLESS OTHERWISE AGREED IN WRITING SIGNED BY THE BAKER COMPANY. THE BAKER COMPANY SHALL NOT BE RESPONSIBLE FOR ANY IMPROPER USE, INSTALLATION, SERVICE OR TESTING OF GOODS

The Baker Company, Inc. P.O. Drawer E Sanford, Maine 04073 Tel: (207) 324-8773 / (800) 992-2537 Fax: (207) 324-3869 Email: bakerco@bakerco.com

www.bakerco.com

Patent pending – Air Bypass Armrest, Cable Port

Baker SterilGARD SG303, SterilGARD SG303-INT, SterilGARD SG303-JPN, SterilGARD SG303-AUS Operator's Manual

Specifications and Main Features

Frequently Asked Questions

User Manual