ANKOM XT10 User Manual
2052 O’Neil Rd, Macedon NY 14502
Telephone: (315) 986-8090
Fax: (315) 986-8091
www.ankom.com
Operator’s Manual
Rev F 12/08/14
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Table of Contents
Introduction .................................................................................................................................................................. 5
Warranty ....................................................................................................................................................................... 5
Filter Bags ..................................................................................................................................................................... 5
Operating Environment ................................................................................................................................................ 5
Contact Information ..................................................................................................................................................... 6
Instrument Description ................................................................................................................................................. 7
Safety Precautions ........................................................................................................................................................ 8
Instrument Installation ................................................................................................................................................. 9
Fat Extraction Calculations ......................................................................................................................................... 10
Fat Extraction Support Items ...................................................................................................................................... 10
Sample Preparation .................................................................................................................................................... 11
Fat Extraction step-by-step procedure using the ANKOM
Periodic Maintenance ................................................................................................................................................. 20
Troubleshooting & Replacement Parts ....................................................................................................................... 22
Diagnostics .................................................................................................................................................................. 23
Appendix A – AOCS Official Fat Extraction Procedure ................................................................................................ 27
Appendix B – Electrical Diagram (pg. 1 of 3) ............................................................................................................... 29
XT10
.................................................................................... 16
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Operator’s Manual
Please review the entire contents of this manual before you begin operating this
product.
Introduction
ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical
laboratories around the world in the environmental, agricultural, biomass, and food industries. ANKOM
Technology can provide you with products for determining or monitoring dietary fiber, detergent fiber, fat,
digestibility, microbial fermentation (anaerobic or aerobic) and more.
Committed to Total Customer Satisfaction, ANKOM designs every product based on a thorough assessment of
customer needs.
Congratulations on your purchase of the ANKOM
effectively serve your needs.
The ANKOM
technician variation, increasing precision. By carefully following the operating instructions in this manual you will
understand the details of sample and filter bag handling as well as the instrument controls, helping you to achieve
the best possible results.
XT10
Extraction System was designed to automate the process of fat extraction. This reduces
XT10
Extraction System. We are confident that this product will
Warranty
ANKOM Technology warrants the ANKOM
or material for one year after the original date of purchase. This warranty does not include damage to the instrument
resulting from neglect or misuse. During the warranty period, should any failure result from defects in
workmanship or materials ANKOM Technology will, at its discretion, repair or replace the instrument free of
charge.
Extended warranties are available upon request.
XT10
Extraction System against any defects due to faulty workmanship
Filter Bags
Use only ANKOM Technology filter bags (part # XT4) in your ANKOM
filter mechanism will void the warranty. Filter bags can be purchased from ANKOM Technology or from your local
authorized ANKOM distributor.
XT10
Extraction System. Use of any other
Operating Environment
Your ANKOM
Ambient Temperature Range: 15°−35°C
Power: 100V−120V ~ 50/60Hz 10A
XT10
Extraction System is designed to operate within the following environments:
220V−240V ~ 50/60Hz 5A
Rev F 12/08/14 pg. 5
Operator’s Manual
Contact Information
At ANKOM Technology we are committed to your total satisfaction and therefore always available to help you get
the most from your ANKOM products. We are also very interested in any comments or suggestions you may have
to help us improve.
For any questions or suggestions regarding your instrument, please contact us at:
Telephone: (315) 986-8090
Fax: (315) 986-8091
Email: service@ankom.com
www.ankom.com
pg. 6 Rev F 12/08/14
Operator’s Manual
Keypad
Display
Power Switch (not visible)
Safety Valve Handle
Pressure Gauge
Extraction Vessel
Solvent Recovery Bottle
Main Handle
Reflux Water Flow Control
Instrument Description
General Description
The ANKOM
petroleum ether. The compounds extracted are primarily triacylglycerols together with a small quantity of related
lipids, traditionally termed “Crude Fat.” The analysis is achieved by measuring the loss of mass due to the
extraction of fat/oil from the sample encapsulated in a filter bag. The quantitative isolation of the sample is
accomplished by surrounding the sample in a sealed filter bag with a filtering capacity in the 2 to 3 micron range.
The filter bag has sufficient porosity to permit rapid solvent passage and is composed of polymeric material that is
resistant to the higher temperatures and solvents used in the instrument.
XT10
Extraction System is designed to extract Crude (Free) Fat using conventional solvents, typically
The quantitative isolation achieved by the filter bag permits the ANKOM
process samples in batches of up to 15. Simply place filter bags with sample in the Teflon Cup, add solvent to the
Extraction Vessel, place the Teflon Cup on top of the solvent in the Extraction Vessel, lock the vessel in place,
select the extraction time and temperature, and press “Start.” The instrument will automatically extract the fat from
the samples and recover the solvent from the Extraction Vessel when the operation is complete.
Below is a detailed view of the ANKOM
XT10
Extraction System.
XT10
Extraction System to automatically
Rev F 12/08/14 pg. 7
Operator’s Manual
Flammable Hazard: Static electricity is to be avoided as it is a spark hazard.
Hot Surfaces: Do not touch the extraction vessel during operation. The surface
can exceed 70°C (158°F). Failure to observe this caution may result in injury.
Hazardous Voltages: Do not operate the instrument with the electrical
compartment open. Hazardous voltages are present during operation. Failure to
observe this caution may result in electrical shock or electrocution.
Hazardous Materials: When adding solvents, follow fill instructions completely
to avoid fire and/or explosion. Waste material will contain small amounts of
volatile solvents. Use caution when discarding. Follow safe laboratory practices
according to both local and federal regulations when installing this instrument
and when handling any organic solvents.
WARNING: Attempts to override safety features or to use this instrument in a
manner not specified by ANKOM Technology voids the warranty and may result
in serious injury or even death.
This system is designed to meet and/or exceed the applicable standards of CE,
CSA, NRTL and OSHA.
Please review the entire contents of this manual before you begin operating this
instrument.
Safety Precautions
pg. 8 Rev F 12/08/14
Operator’s Manual
1.
Remove the instrument from the shipping container and place it on a smooth level surface in an area that
is free of dust and excessive moisture and is within 6 ft. of a drain and cold water supply.
2.
Place the instrument either in a Vent Hood or within 6 ft. of one.
The user is responsible for determining if venting of this instrument requires
placement in a hood. Please follow both local and federal regulations when
installing this instrument.
3.
Connect the tube from the top fitting to the water supply.
4.
Connect the tube from the bottom fitting to the drain.
5.
Connect the Solvent Recovery Bottle to the instrument.
Ensure that there are no kinks or bends in any of the tubing connected to the
instrument.
6.
Plug the power cord into a grounded receptacle.
Drain
Water Supply
Vent Tube
Solvent Recovery Bottle
Instrument Installation
Site Requirements
To install and operate the ANKOM
Adjustable wrench
Chilled or tap water supply not to exceed 23°C
Adequate power (see “Operating Environment” section)
Drain
Vent Hood
Instrument Installation Procedure
To install the ANKOM
XT10
Extraction System, follow the procedure detailed below.
XT10
Extraction System you will need the following:
Rev F 12/08/14 pg. 9
Operator’s Manual
Crude Fat contained within a food or feed sample can be calculated using the following formula:
% Crude Fat
=
100 x (W2 – W3)
W1
Where:
W1 = Original weight of sample
W2 = Weight of pre-extraction dried sample and filter bag
W3 = Weight of dried sample and filter bag after extraction
For Liquid Samples, W2 and W3 in the equation above will include the weight of Diatomaceous Earth (DE).
For meat samples with > 15% fat and plant samples with > 20% fat:
% Crude Fat
=
100 x ((W2 – W4) – W3)
W1
Where:
W4 = Weight of the weigh tin
If hydrolysis is done prior to fat extraction, then Total Fat contained within a food or feed sample can be calculated
using the following formula:
% Total Fat
=
100 x (W2 – (W3 + (C1 – C2)))
W1
Where:
W1 = Original weight of sample
W2 = Weight of dried sample, filter bag, and Diatomaceous Earth (DE) after hydrolysis
W3 = Weight of dry extracted sample, filter bag, and DE
C1 = Blank filter bag dry weight after hydrolysis
C2 = Blank filter bag weight after extraction
Item
Recommended Product
Electronic Balance with four-place readout
ANKOM #TB Balance Hardware
ANKOM #TBS Balance Software
Filter Bags
ANKOM #XT4
Bag Holder (used for adding sample to an empty filter bag)
ANKOM #101.2
Heat Sealer for sealing the filter bags
ANKOM #1915 (120V), #1920 (220V)
Solvent Resistant Marker
ANKOM #F08
Desiccant Pouch or Weigh Tin Desiccator for drying
ANKOM #X45, #X49
Dryer (capable of temperatures from 60-120°C) - optional
ANKOM #RD (120V), #RDI (220V)
Oven for drying (capable of maintaining 102°C±2°)
Ventilated oven trays to allow for sufficient oven air flow
Weigh Tins (used with samples of > 20% fat)
Sample
Spoon
Fat Extraction Calculations
Crude Fat Calculation
Total Fat Calculation
Fat Extraction Support Items
The following support items are needed to perform the fat extraction procedure:
pg. 10 Rev F 12/08/14
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