ANKOM HCl User Manual

Operator’s Manual
Rev L 11/12/14
2052 O’Neil Rd, Macedon NY 14502 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.com
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Table of Contents
Introduction .................................................................................................................................................................. 5
Warranty ....................................................................................................................................................................... 5
Filter Bags ..................................................................................................................................................................... 5
Operating Environment ................................................................................................................................................ 5
Contact Information ..................................................................................................................................................... 6
Instrument Description ................................................................................................................................................. 7
Safety Precautions ........................................................................................................................................................ 8
Instrument Installation and Setup ................................................................................................................................ 9
Hydrolysis Procedure .................................................................................................................................................. 13
Periodic Maintenance ................................................................................................................................................. 21
Troubleshooting .......................................................................................................................................................... 23
Appendix A – Electrical Diagram (pg. 1 of 3) .............................................................................................................. 25
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Operator’s Manual
Rev L 11/12/14
pg. 5
The ANKOM
HCl
and XT Extractors are coated with acid resistant polymer to maintain the appearance of the system. Allowing these systems to be subjected to acid contact for long periods of time will affect their appearance and should be avoided. Regular attention to periodic maintenance and proper set-up will assure long life.
Please review the entire contents of this manual before you begin operating this product.
Introduction
ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical laboratories around the world in the food, feed, bio-energy, agricultural, and environmental industries. ANKOM Technology can provide you with products for determining or monitoring dietary fiber, detergent fiber, crude fiber, fat, digestibility, microbial fermentation (anaerobic or aerobic) and more.
Committed to Total Customer Satisfaction, ANKOM designs every product based on a thorough assessment of customer needs.
Congratulations on your purchase of the ANKOM effectively serve your needs.
By carefully following the operating instructions in this manual, you will minimize errors in results. Experience indicates that errors in results are usually associated with minor variations in carrying out the procedure. This manual will provide you with details that will help assure accuracy of your results.
HCl
Hydrolysis System. We are confident that this product will
Warranty
ANKOM Technology warrants the ANKOM or material for one year after the original date of purchase. This warranty does not include damage to the instrument resulting from neglect or misuse. During the warranty period, should any failure result from defects in workmanship or materials, ANKOM Technology will, at its discretion, repair or replace the instrument free of charge.
Extended warranties are available upon request.
HCl
Hydrolysis System against any defects due to faulty workmanship
Filter Bags
For optimal function, only ANKOM Technology filter bags (part # XT4) should be used in your ANKOM Hydrolysis System. Filter bags can be purchased from ANKOM Technology or from your local authorized ANKOM distributor.
Operating Environment
Your ANKOM
Ambient Temperature Range: 15°−35°C Power (domestic): 100V120V ~ 50/60Hz 10A Power (international): 220V240V ~ 50/60Hz 5A
HCl
Hydrolysis System is designed to operate within the following environments:
HCl
Operator’s Manual
pg. 6
Rev L 11/12/14
Contact Information
ANKOM Technology is committed to your total satisfaction and therefore always available to help you get the most from your ANKOM products. We are also very interested in any comments or suggestions you may have to help us improve.
For any questions or suggestions regarding your instrument, please contact us at:
Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.com
Operator’s Manual
Rev L 11/12/14
pg. 7
Fat extraction can be done using the ANKOM
XT15
and ANKOM
XT10
Extractors.
Top Cover (electrical compartment)
Display
Vessel Lid
Hydrolysis Vessel
Vent Hose
Water Supply Connection
Water Drain Hose
Keypad
Lid Latch
Instrument Description
General Description
The ANKOM the same time in an automated fashion using acid hydrolysis so that the fat from the samples can then be extracted using fat solvents.
Below is a detailed exterior view of the ANKOM
HCl
Hydrolysis System is designed to release complexed lipids from multiple food or feed samples at
HCl
Hydrolysis System.
Operator’s Manual
pg. 8
Rev L 11/12/14
Hot Surfaces – Do not touch the Vessel Lid during operation. The surface can exceed 70°C (158°F). Failure to observe this caution may result in injury.
Hazardous Voltages Do not operate the instrument with the Top Cover (electrical compartment) open. Hazardous voltages are present during operation. Failure to observe this caution may result in electrical shock or
electrocution.
Hazardous MaterialsAcid solution is corrosive. Do not allow acid solution
to contact the skin. Use caution when discarding waste. Dispose of waste in accordance with safe practices and regulatory provisions.
WARNING: Attempts to override safety features or to use the instruments in a
manner not specified by the manufacturer voids the warranty and may result in serious injury or even death.
This system is designed to meet and/or exceed the applicable standards of CE and CSA.
Please review the entire contents of this manual before you begin operating this instrument.
Safety Precautions
Operator’s Manual
Rev L 11/12/14
pg. 9
If you use a dryer, remember to regularly check the air flow through the filter. For details, refer to the operator’s manual for your dryer.
1.
Remove the instrument from the shipping container and set it on a smooth level surface near a drain and a cold water supply.
WARNING: Due to the corrosive nature of hydrochloric acid, it is recommended that the instrument be operated in an acid ventilation hood separate from other instruments that are subject to corrosion.
2.
Connect the instrument to a water supply using the “Water Supply Connection.”
3.
Install the “Water Drain Hose” and position it to run downhill from the instrument to a drain, allowing for proper drainage and preventing condensation from accumulating inside.
Water Drain Hose
Water Supply Connection
Vent Hose
Instrument Installation and Setup
Site Requirements
To install and operate the ANKOM
Adjustable wrench Tap water connection Adequate power (see “Operating Environment” section)
Drying capability
It is extremely important that you completely dry your food or feed samples in an ANKOMRD Dryer or an oven resistant to HCl fumes. If samples are not dried enough, residual water and acid left in the samples will lead to inaccurate fat values. Overheating samples can lead to complexing of the fats resulting in lower values.
The ANKOMRD Dryer is specifically designed for hydrolysis samples and should be set at 110°C. This will result in the appropriate temperature of 102°C inside the bag. If you are drying samples using a laboratory oven, it is recommended that you use a mechanical convection oven set to 102°C±2°.
Instrument Installation
The ANKOM instrument’s power cord must be plugged into a grounded receptacle. Ensure there are no kinks or bends in any of the tubing that is connected to the instrument.
To install the ANKOM
HCl
Hydrolysis System should be installed in a clean area free of dust and excessive moisture. The
HCl
Hydrolysis System, follow the procedure detailed below.
HCl
Hydrolysis System you will need the following:
Operator’s Manual
pg. 10
Rev L 11/12/14
4.
Attach the HCl Zinc Filter Assembly (part# H36) to the “Vent Hose” as shown below. Position the hose and the filter to run downhill from the instrument to prevent condensation from accumulating inside.
Replace the filter every four months or when pH paper placed at the end of the filter indicates that acid fumes are exiting the filter.
5.
Plug the power cord into a grounded receptacle.
Water Drain Hose
Vent Hose
HCl Zinc Filter Assembly (H36)
Operator’s Manual
Rev L 11/12/14
pg. 11
This step is extremely important to fill the waste tank in the instrument with water to dilute the waste acid from the first and subsequent runs.
1.
Turn the instrument power switch ON. When the power is on, the switch light and the power light on the Keypad are lit.
2.
Turn the water supply on.
3.
Pull up on the Lid Latch to open the Hydrolysis Vessel.
4.
Pour enough water into the Hydrolysis Vessel to cover the ports in the back.
5.
Using the UP/DOWN arrows on the Keypad, enter an extraction time of 20 minutes.
6.
Press ENTER on the Keypad.
7.
Using the UP/DOWN arrows on the Keypad, enter a temperature of 90°C.
8.
Press ENTER on the Keypad.
9.
Using the UP/DOWN arrows on the Keypad, enter a rinse time of 10 minutes.
10.
Press ENTER on the Keypad.
11.
Close the Vessel Lid (a message on the Display will remind you to do this).
12.
Press START on the Keypad. Within 20 minutes the water should heat to 90°C. Then the Water Drain will open.
13.
Make sure that all of the water has drained out of the Hydrolysis Vessel. You should then observe two rinses with each rinse taking approximately 5 minutes to complete.
Repeat the above procedure every three months to verify that the instrument is functioning properly.
Keypad
Vessel Lid
Lid Latch
Display
Hydrolysis Vessel
Instrument Setup
To get your ANKOM
HCl
Hydrolysis System set up and ready for use, follow the procedure detailed below.
Operator’s Manual
pg. 12
Rev L 11/12/14
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Operator’s Manual
Rev L 11/12/14
pg. 13
% Total Fat
=
100 x (W2 – (W3 + (C1 – C2)))
W1
Where:
W1 = Original weight of sample
W2 = Weight of dried sample, filter bag, and Diatomaceous Earth (DE) after hydrolysis
W3 = Weight of dry extracted sample, filter bag, and DE
C1 = Blank filter bag dry weight after hydrolysis
C2 = Blank filter bag weight after extraction
Item
Recommended Product
Electronic Balance with four-place readout
ANKOM #TB Balance Hardware ANKOM #TBS Balance Software
Filter Bags
ANKOM #XT4
Bag Holder (used for adding sample to an empty filter bag)
ANKOM #101.2
Diatomaceous Earth (DE)
ANKOM #XTC
Heat Sealer for sealing the filter bags
ANKOM #1915 (120V), #1920 (220V)
Solvent Resistant Marker
ANKOM #F08
Multi-bag Holder (used to hold bags in the Hydrolysis Chamber)
ANKOM #H33
Desiccant Pouch for drying
ANKOM #X45
Oven for drying
ANKOM #RD (120V), #RDI (220V)
Sample
Spoon
Hydrolysis Procedure
Total Fat Calculation
The hydrolysis procedure is done prior to fat extraction. When both hydrolysis and fat extraction are done, the Total Fat contained within a food or feed sample can be calculated using the following formula:
Hydrolysis Support Items
The following support items are needed to perform the hydrolysis procedure:
Operator’s Manual
pg. 14
Rev L 11/12/14
For best results, it is important to determine the correct ratio of Diatomaceous Earth (DE) to sample. Before using your ANKOM
HCl
Hydrolysis System for production testing, it is recommended that you categorize your samples into types and determine the standard amount of DE needed for each sample type. The following information is from testing done in the ANKOM lab. This information is meant to help you categorize your own samples.
Sample Type
Fat %
DE
Weight
Sample Weight
Notes
Yogurt
1 - 1.5 %
0.75 g
0.75 g
Whole Milk
~ 3%
1.25 g
1.25 g
After adding the DE to the filter bag, press the eraser end of a pencil into the DE to create an indentation into which the milk can be added using a pipette. Record the weight (to make sure DE was not transferred to the pencil) & tare.
Whipping Cream
35%
1.25 g
0.5 g
After adding cream to the filter bag using a pipette (see milk technique above) and not overflowing the indentation in the DE, leave the filter bag open and dry it in an oven for 1 hour at 100-105°C. Seal the bag.
Whey Powder
2%
0.75 g
0.75 g
Veggie Chips
20%
0.9 g
0.6 g
Tortilla Chips
25%
0.9 g
0.6 g
Swiss Cheese
30%
1.0 g
0.5 g
Soybean
20%
0.75 g
0.75 g
Sour Cream
20%
0.8 g
0.7 g
Processed Cheese
20 - 25 %
1.0 g
0.5 g
Processed Cheese
> 25 %
1.2 g
0.3 g
Pretzels
5%
0.5
1.0 g
Powdered Non-Dairy Creamer
35%
1.0 g
0.5 g
Potato Chips
30 - 40 %
1.2 g
0.3 g
Mozzarella Cheese
20%
1.0 g
0.5 g
Moist Pet Food (canned)
< 10 %
0.75 g
0.75 g
Moist Distillers Grain (syrup)
10 - 12 %
0.7 g
1.0 g
Milk Replacer
> 30%
0.9 g
0.6 g
Milk Replacer
20 - 30 %
0.75 g
0.75 g
Milk Powder
15 - 20 %
0.75 g
0.75 g
Milk Chocolate
25 - 30 %
0.8 g
0.7 g
Meat & Bone Meal
10 - 15 %
0.5 g
1.0 g
Mayonnaise
75 - 80 %
1.2 g
0.3 g
Hot Dog
30%
1.2 g
0.3 g
Ground Beef
< 20 %
1.0 g
0.5 g
Ground Beef
> 20 %
1.2 g
0.3 g
Forages
2 - 3 %
0.3 g
1.2 g
Most forages are light. Do not allow the mass in the bag to puff out the sides of the filter bag which will make it more difficult to completely saturate the sample.
Fish Feed
15%
0.75 g
0.75 g
Feed Mixtures
< 10%
0.3 - 0.4 g
1.1 - 1.2 g
Dried Pet Food
5 - 15 %
0.5 g
1.0 g
Dried Distillers Grain
< 15 %
0.5 g
1.0 g
Dried Algae
15 - 20 %
0.8 g
0.7 g
Cream Cheese
15 - 35 %
1.0 g
0.5 g
Cottage Cheese
< 5 %
0.6 g
0.9 g
Chocolate Liquor
> 50 %
1.1 g
0.4 g
Cheese Curls
~ 25 %
0.9 g
0.6 g
Cheddar Cheese
35%
1.2 g
0.3 g
Sample Categorization
Sample Categorization Data from ANKOM Lab (sorted in alphabetical order by Sample Type)
Operator’s Manual
Rev L 11/12/14
pg. 15
Sample Type
Fat %
DE Weight
Sample Weight
Notes
Whole Milk
~ 3%
1.25 g
1.25 g
After adding the DE to the filter bag, press the eraser end of a pencil into the DE to create an indentation into which the milk can be added using a pipette. Record the weight (to make sure DE was not transferred to the pencil) & tare.
Whipping Cream
35%
1.25 g
0.5 g
After adding cream to the filter bag using a pipette (see milk technique above) and not overflowing the indentation in the DE, leave the filter bag open and dry it in an oven for 1 hour at 100-105°C. Seal the bag.
Processed Cheese
> 25 %
1.2 g
0.3 g
Potato Chips
30 - 40 %
Mayonnaise
75 - 80 %
Hot Dog
30%
Ground Beef
> 20 %
Cheddar Cheese
35%
Chocolate Liquor
> 50 %
1.1 g
0.4 g
Swiss Cheese
30%
1.0 g
0.5 g
Processed Cheese
20 - 25 %
Powdered Non-Dairy Creamer
35%
Mozzarella Cheese
20%
Ground Beef
< 20 %
Cream Cheese
15 - 35 %
Veggie Chips
20%
0.9 g
0.6 g
Tortilla Chips
25%
Milk Replacer
> 30%
Cheese Curls
~ 25 %
Sour Cream
20%
0.8 g
0.7 g
Milk Chocolate
25 - 30 %
Dried Algae
15 - 20 %
Yogurt
1 - 1.5 %
0.75 g
0.75 g
Whey Powder
2%
Soybean
20%
Moist Pet Food (canned)
< 10 %
Milk Replacer
20 - 30 %
Milk Powder
15 - 20 %
Fish Feed
15%
Moist Distillers Grain (syrup)
10 - 12 %
0.7 g
1.0 g
Cottage Cheese
< 5 %
0.6 g
0.9 g
Meat & Bone Meal
10 - 15 %
0.5 g
1.0 g
Dried Pet Food
5 - 15 %
Dried Distillers Grain
< 15 %
Pretzels
5%
Forages
2 - 3 %
0.3 g
1.2 g
Most forages are light. Do not allow the mass in the bag to puff out the sides of the filter bag which will make it more difficult to completely saturate the sample.
Feed Mixtures
< 10%
0.3 - 0.4 g
1.1 - 1.2 g
Sample Categorization Data from ANKOM Lab (sorted by DE Weight)
Operator’s Manual
pg. 16
Rev L 11/12/14
To avoid fat migration from one filter bag to another during the hydrolysis procedure, it is very important to prepare your samples properly.
Before running production samples, it is recommended that you do the following:
Categorize your samples to determine the standard amount of DE needed
for each of your specific sample types. See the “Sample Categorization” section for details.
Mix dry/granular samples with the correct amount of DE. Cover moist samples with the correct amount of DE.
To prepare samples for hydrolysis, follow the procedure detailed below.
1.
Using a Solvent Resistant Marker, number all of the empty filter bags you will use during the hydrolysis procedure.
2.
Record the weights of all the filter bags prior to filling them.
3.
Place an empty filter bag in the Bag Holder in an open position.
4.
Tare the weight of the empty filter bag and the holder together.
5.
Add the necessary DE and sample to the filter bag for Dry/Granular, Moist, and Liquid samples. (See the “Sample Categorization” section for help in determining how much DE to add to the filter bag.)
For Dry/Granular or Moist samples:
The total weight of DE and sample dry matter must NOT exceed 1.5g. Too much volume inside the bags will make it difficult for the acid to completely saturate the sample during hydrolysis. This may reduce the number of bags that can be processed in the Hydrolysis Vessel at one time.
For Liquid samples:
The total weight of DE and sample MAY exceed 1.5g.
Dry/Granular Samples
a. Add the full recommended amount of DE to the filter bag. b. Record the weight of the DE and tare. c. Add an appropriate amount of sample to the filter bag. Keep all
particles away from the sealing area of the filter bag.
d. Record the weight of the sample (W1).
Sample Preparation Procedure
Operator’s Manual
Rev L 11/12/14
pg. 17
Moist Samples
a. Add 1/2 of the recommended amount of DE to the filter bag. b. Using a spatula, make a valley or a space in the DE for the sample. c. Record the weight and tare. d. Place an appropriate amount of sample into the DE valley in the filter
bag, being careful that the sample does not touch the inside walls of the filter bag so the sample can be fully covered with DE.
e. Record the weight of the sample (W1) and tare. f. Add the rest of the DE to the filter bag to completely cover the sample. g. Record the weight of the DE.
Liquid Samples
a. Add the full recommended amount of DE to the filter bag. b. Using a pencil eraser (or similar sized utensil), make an indentation or
a space in the DE into which the liquid sample can be added using a pipette.
c. Record the weight and tare. d. Pipette the appropriate amount of liquid sample into the DE
indentation in the filter bag.
e. Record the weight of the sample (W1).
6.
Set the Heat Sealer dial to 6. (The setting may vary from sealer to sealer.)
7.
Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of the filter bag (as shown to the right). If the seal is not strong, re-seal the bag.
8.
For Dry/Granular or Moist samples, squeeze the edges of the filter bag then shake it to mix or cover the sample with the DE. Flatten the bag after shaking.
9.
If the sample is liquid or clumpy (for example ground beef) and makes the filter bag bulge, gently squeeze the bag at the bulge location to flatten it.
10.
Repeat steps 3 – 9 for all filter bags that will be used in the ANKOM
HCl
Hydrolysis System. (Up to 15
bags can be processed during one procedure.)
Seal
Operator’s Manual
pg. 18
Rev L 11/12/14
11.
Include two blank bags (C1 and C2) filled with approximately 0.5g – 0.75g of DE.
Blank bags serve as correction factors for slight losses of weight that may occur during extraction. Blank bags with DE also act as indicators of fat loss during hydrolysis. When placed next to a sample that loses fat, a blank bag will absorb some of the fat lost by the other bag. The result is a blank loss during extraction that exceeds the normal expected blank loss of 0.0025g by
0.0005g or more. This indicates that fat migrated from a bag with sample to a blank bag during hydrolysis, and then that same fat was lost during extraction. If fat migrates from the bags, a pre-extraction with solvent may be necessary.
12.
Insert filter bags into the Multi-bag Holder and snap the handle in place. Samples are now ready for the hydrolysis procedure.
Operator’s Manual
Rev L 11/12/14
pg. 19
1.
Turn the instrument power switch ON.
2.
Turn the cold water supply on.
3.
Pull the Lid Latch up to open the Vessel Lid.
4.
Place the Multi-bag Holder into the Hydrolysis Vessel.
5.
Pour 500ml of 3N HCl into the Hydrolysis Vessel to cover the ports in the back.
6.
Roll up a piece of paper towel and place it on the Vessel Lid just above the Hinge as shown to the right. This will help prevent drips of liquid from getting on the Hinge after a run.
7.
Push the Lid Latch down to close the Vessel Lid.
8.
Press ENTER on the Keypad.
9.
Use the UP/DOWN arrows on the Keypad to enter the hydrolysis time (suggested time is 60 minutes).
10.
Press ENTER on the Keypad.
11.
Use the UP/DOWN arrows on the Keypad to enter the hydrolysis temperature (suggested temperature is 90°C).
12.
Press ENTER on the Keypad.
13.
Use the UP/DOWN arrows on the Keypad to enter the rinse time (suggested time is 20 minutes).
14.
Press ENTER on the Keypad.
15.
Make sure the Vessel Lid is closed (a message on the Display will remind you to do this).
Display
Keypad
Lid Latch
Vessel Lid
Hinge on Vessel Lid
Hydrolysis Vessel
Rolled-up Paper Towel
Ports in back of Vessel
Hydrolysis step-by-step procedure using the ANKOM
HCl
Hydrolysis System
To perform hydrolysis on prepared samples, follow the procedure detailed below.
Operator’s Manual
pg. 20
Rev L 11/12/14
16.
Press START on the Keypad. The ANKOM
HCl
Hydrolysis System will automatically heat and maintain the set temperature. After hydrolysis is complete, the samples are automatically rinsed with cold water. When the instrument has completed the automated process, the Display will show “Process Complete”.
17.
When the automated process is complete, open the Lid Latch and immediately wipe the Teflon pad on the underside of the lid with paper towel.
This step is extremely important to prevent acid from corroding the hinge on the Vessel Lid.
18.
Remove the Multi-bag Holder from the Hydrolysis Vessel.
19.
Remove the individual filter bags from the Multi-bag Holder and place them on four layers of paper towel.
20.
Place two layers of paper towel on top of the wet filter bags. Using the ANKOM Blotter, apply uniform pressure to the top of the paper towels for two minutes to gently press the water out of the filter bags. Blotting helps to reduce the drying time and the amount of acid residue in the bags.
21.
Repeat the blotting process for two more minutes before drying.
22.
Completely dry the samples in an ANKOMRD Dryer or in an oven resistant to HCl fumes. (ANKOM recommends drying for around three hours at around 100°C.)
Residual acid left in the samples due to incomplete drying will cause corrosion in the extractor and artificially high fat values.
23.
Remove samples from the dryer/oven and place them directly into a Desiccant Pouch containing pH paper.
24.
Allow samples to cool to room temperature and check the pH paper. If the presence of acid shows on the paper, continue drying. If the pH paper is neutral, record weight (W2) of samples.
If your hydrolysis results are low, it is possible that the acid was not able to completely saturate and hydrolyze the sample. In this case, it may be necessary to use no more than 1.0g of the combination of sample and DE in your filter bags. Depending on the sample content, a possible combination could be 0.5g of sample and 0.5g of DE.
Your samples are now ready for fat extraction in an ANKOM
XT15/XT10
Extractor. See the specific extractor Operator’s Manual for instructions.
Operator’s Manual
Rev L 11/12/14
pg. 21
Do NOT use Acetone to clean the instrument.
Periodic Maintenance
Cleaning the exterior of the instrument
At the end of each day use an ammonia-based cleaner (a mild base solution) and cloth to wipe the outside of the instrument, making sure to clean the hinge on the Vessel Lid.
Cleaning the interior of the Vessel
To restore the white color to the Vessel, periodically run a bleach solution through the instrument using the procedure detailed below.
1. Turn the water supply on.
2. Pull the Lid Latch up to open the Vessel Lid.
3. Pour 600 ml or enough of a bleach solution into the Hydrolysis Vessel to cover the two ports in the back
wall of the vessel.
4. Push the Lid Latch down to close the Vessel Lid.
5. Press ENTER on the Keypad.
6. Use the UP/DOWN arrows on the Keypad to enter a hydrolysis time of 60 minutes.
7. Press ENTER on the Keypad.
8. Use the UP/DOWN arrows on the Keypad to enter a hydrolysis temperature of 90°C.
9. Press ENTER on the Keypad.
10. Use the UP/DOWN arrows on the Keypad to enter a rinse time of 20 minutes.
11. Press ENTER on the Keypad.
12. Make sure the Vessel Lid is closed (a message on the Display will remind you to do this).
13. Press START on the Keypad. The bleach solution will automatically run through the instrument. When the
process has completed, the Display will show “Process Complete.”
Replacing the HCl Fuses
To replace the fuses in the ANKOM
1. Turn off the instrument power and unplug the Power Cord from the outlet.
2. Remove the two screws on the Top Cover and set them aside.
3. Swing the Top Cover down exposing the electrical compartment.
4. Locate the Fuse Holders on the terminal strip (see the image on the following page). There are two fuses
within the instrument; one for each pole (100 – 120V 10A or 220 – 240V 5A).
HCl
Hydrolysis System, follow the procedure detailed below.
Operator’s Manual
pg. 22
Rev L 11/12/14
Tab to open Fuse Holders
Fuse
Note the arrow orientation
Fuse Holders
5. Press the tab and lift the Fuse Holder up.
6. Replace the fuses and close the Fuse Holder completely.
7. Close the Top Cover and secure it with the two screws that you previously removed.
Cleaning/Checking the Water Filter
To clean/check the Water Filter in the ANKOM
1. Unscrew the Water Supply Connection and barb fitting from the HCl instrument.
2. Use compressed air to clean the filter.
3. Reassemble using Teflon tape on the threads.
HCl
Hydrolysis System, follow the procedure detailed below.
Operator’s Manual
Rev L 11/12/14
pg. 23
Troubleshooting & Replacement Parts
The ANKOM Technology web site has the most current troubleshooting and replacement parts information. If you have any questions about the operation of your ANKOM please visit www.ankom.com.
HCl
Hydrolysis System, or if you need replacement parts,
Operator’s Manual
pg. 24
Rev L 11/12/14
This page intentionally left blank
Operator’s Manual
Rev L 11/12/14
pg. 25
Appendix A – Electrical Diagram (pg. 1 of 3)
Operator’s Manual
pg. 26
Rev L 11/12/14
Appendix A – Electrical Diagram (pg. 2 of 3)
Operator’s Manual
Rev L 11/12/14
pg. 27
Appendix A – Electrical Diagram (pg. 3 of 3)
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RF Gas Production System
High sensitivity pressure measurement Anaerobic activity analyses (rumen, yeast, beer/wine fermentation, etc.) Biodegradability analyses
Aerobic activity analyses (BOD, soil respiration, etc.)Wireless Computer control and data storage
Chemicals
A wide variety of chemicals used for many different lab operations
Pre-mixed solutions available
Please visit our web site at www.ankom.com for more information.
2052 O’Neil Rd, Macedon NY 14502 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.com
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