ANKOM 2000 User Manual
Operator’s Manual
2052 O’Neil Rd, Macedon NY 14502
Telephone: (315) 986-8090
Fax: (315) 986-8091
www.ankom.com
Rev E 1/14/15
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Table of Contents
Introduction .................................................................................................................................................................. 5
Warranty ....................................................................................................................................................................... 5
Filter Bags ..................................................................................................................................................................... 5
Operating Environment ................................................................................................................................................ 5
Contact Information ..................................................................................................................................................... 6
Instrument Description ................................................................................................................................................. 7
Safety Precautions ...................................................................................................................................................... 10
Instrument Installation ............................................................................................................................................... 11
Fiber Analysis Support Items ...................................................................................................................................... 13
Analysis Options using the ANKOM
ADF Analysis ................................................................................................................................................................ 15
NDF Analysis ............................................................................................................................................................... 19
Crude Fiber Analysis ................................................................................................................................................... 25
Flush Procedure .......................................................................................................................................................... 31
Periodic Maintenance ................................................................................................................................................. 33
Troubleshooting .......................................................................................................................................................... 36
Appendix A – ADF Method ......................................................................................................................................... 37
Appendix B – NDF Method ......................................................................................................................................... 39
Appendix C – Crude Fiber Method ............................................................................................................................. 41
Appendix D – Parts & Assemblies ............................................................................................................................... 45
2000
Fiber Analyzer ............................................................................................... 13
Appendix E – Wiring Diagrams (1 of 4) ....................................................................................................................... 48
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Operator’s Manual
Please review the entire contents of this manual before you begin operating this
product.
Introduction
ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical
laboratories around the world in the environmental, agricultural, biomass, and food industries. ANKOM
Technology can provide you with products for determining or monitoring detergent fibers, dietary fibers, fat,
digestibility, microbial fermentation (anaerobic or aerobic) and more.
Committed to Total Customer Satisfaction, ANKOM designs every product based on a thorough assessment of
customer needs.
Congratulations on your purchase of the ANKOM
effectively serve your needs.
By carefully following the operating instructions in this manual, you will minimize errors in results. Experience
indicates that errors in results are usually associated with minor variations in carrying out the procedure. This
manual will provide you with details that will help assure accuracy of your results.
2000
Fiber Analyzer. We are confident that this product will
Warranty
ANKOM Technology warrants the ANKOM
material for one year after the original date of purchase. This warranty does not include damage to the instrument
resulting from neglect or misuse. If the instrument is damaged as a result of defects in the workmanship or
materials during the warranty period, ANKOM Technology will repair or replace the instrument free of charge.
Extended warranties are available for purchase if desired.
2000
Fiber Analyzer against any defects due to faulty workmanship or
Filter Bags
ANKOM Technology filter bags (part # F57) are designed to support precision and accuracy in analysis. Use of
other types of filtration media not tested and approved by ANKOM Technology may cause damage to electrical
valves and other components and void your warranty. Filter bags can be purchased from ANKOM Technology or
from your local authorized ANKOM distributor.
Operating Environment
Your ANKOM
Ambient Temperature Range: 15°−30°C
Humidity: 20–60% RH
Power (domestic): 110V−120V ~ 50/60Hz 15A
Power (international): 220V−240V ~ 50/60Hz 10A
2000
Fiber Analyzer is designed to operate within the following environments:
Rev E 1/14/15 pg. 5
Operator’s Manual
Contact Information
At ANKOM Technology we are committed to your total satisfaction and therefore always available to help you get
the most from your ANKOM products. We are also very interested in any comments or suggestions you may have
to help us improve.
For any questions or suggestions regarding your instrument, please contact us at:
Telephone: (315) 986-8090
Fax: (315) 986-8091
Email: service@ankom.com
www.ankom.com
pg. 6 Rev E 1/14/15
Operator’s Manual
Keypad
Display
Vessel Lid
Vessel
Vessel Clamp Handle
Temperature Controller
Power Cord
Power Switch
Instrument Description
General Description
The ANKOM
Neutral Detergent Fiber (NDF), and Crude Fiber within food and/or feed samples. Enabled by Filter Bag
Technology, up to 24 samples can be processed at one time.
During analysis cell contents are removed as the encapsulated sample is subjected to the appropriate chemical (AD,
ND, or crude fiber acid and base) solutions, leaving the desired fiber fraction. Results are determined
gravimetrically. The filter bags are designed to allow proper flow of solutions while retaining non-soluble
components. The fiber residue captured in the filter bag can be used for follow-on assays such as ADIN, NDIN, and
ADL.
2000
Fiber Analyzer is designed to efficiently and accurately determine Acid Detergent Fiber (ADF),
Like the ANKOM
200
Fiber Analyzer, digestion and rinse operations are all performed within the same instrument,
allowing for the elimination of the separate filtration step. Process temperatures are precisely controlled while
providing proper agitation to ensure a uniform flow of chemical solutions and rinses across each sample.
Below are detailed views of the ANKOM
2000
Fiber Analyzer.
Rev E 1/14/15 pg. 7
Operator’s Manual
Power Switch
Port B – Used for Crude Fiber Base solution, AD
solution, and Diluted Amylase
Power Cord Inlet
Temperature Controller
Port A – Used for Crude Fiber
Acid solution and ND solution
Fuses
Vent Tube
Line to Hot Water Supply
Drain Hose
Water Filter
Left Side View
Right Side View
Water Supply Fitting
Domestic: 15A/120V
International: 10A/220V
pg. 8 Rev E 1/14/15
Operator’s Manual
A2000 Computer
(part # A09)
Check Valve
Assembly
Pressure Transducer (part # 6121)
&
Fitting Assembly (part # A25)
Valve B
(part # A19 B)
SICK Fiber
Optic
Module - for
Instruments
delivered
before 4/09
(part # A18)
Vent Valve
(part # A22)
24 VDC
Power
Supply
(part # 963)
High Voltage DIN Rail
Drain Valve
Level Sensor
Fitting
ETS Switch
Water
Supply Valve
(part # A21)
Maintenance
Alert
Collector
(part # F27)
Motor Coupler
(part # 632)
24V DIN Rail
Motor
(part # A08 – 120/60)
(part # A10 – 220/50)
Digestion
Vessel
Temperature
Probe
(part # A17)
Analog Level
Sensor
Module - for
Instruments
delivered
after 4/09
(part # A31)
Valve A
(part # A19 A)
Internal Components
Rev E 1/14/15 pg. 9
Operator’s Manual
Hazardous Pressure – Do NOT open the Vessel Lid during operation. The
contents of the Vessel are hot and under pressure. Failure to observe this
caution may result in scalding or burning.
Hot Surfaces – Do NOT touch the Vessel surfaces during operation. The surface
can exceed 70°C (158°F). Failure to observe this caution may result in
burning.
Hazardous Voltages – Do NOT operate the instrument with the cover removed.
Hazardous voltages are present during operation. The Power Cord must be
disconnected prior to removal of the rear panel. Failure to observe this caution
may result in electrical shock or electrocution.
Hazardous Materials – Caution should be used when handling hot effluent that
may be caustic or corrosive. If necessary, the solution can be collected in a
container and neutralized before disposal. Follow safe laboratory practices
according to your local regulations when installing and using this instrument and
associated chemicals.
WARNING: Attempts to override safety features or to use this instrument in a
manner not specified by ANKOM Technology voids the warranty and may result
in serious injury or even death.
This system is designed to meet and/or exceed the applicable standards of
CE, CSA, NRTL and OSHA.
IMPORTANT
The Power Switch must be in the OFF position before plugging the instrument Power
Cord into the power source.
In the event of an instrument malfunction, the internal heater will be automatically
turned off by one of the following safety devices:
1) Electrical Fuses
2) The Emergency Temperature Shut-off Switch (ETS)
3) The Pressure Transducer
Do NOT open the Vessel Lid during or after an operation until both pressure and
liquid are thoroughly exhausted. Connect and secure the Drain Hose along the path to
the drain so it will not move when hot pressurized fluid is exhausted. Failure to
secure the hose could result in uncontrolled chemical flow.
Please review the entire contents of this manual before you begin operating this
instrument.
Safety Precautions
pg. 10 Rev E 1/14/15
Operator’s Manual
1.
Remove the instrument from the shipping container and place it in an area that is within six feet of a
drain and water supply on a surface that is firm and level. The instrument must not be subject to
excessive shock, vibration, dirt, moisture, oil, or other fluids.
IMPORTANT
Do NOT place this instrument near microwave ovens or mechanical devices.
Your instrument comes complete with a Power Cord, a Drain Hose, a Vent Tube, a Bag Suspender
Assembly (including Bag Suspender Trays and a Bag Suspender Weight), and an Amylase Container.
2.
With the Power Switch in the OFF position, plug the Power Cord into the Power Cord Inlet.
3.
Plug the Power Cord into the power source.
4.
Install the Water Filter assembly. Attach ¼” copper tubing to the hot water source (+50°C Crude Fiber,
+70°C ND and AD) and to the Water Supply Fitting located on the left side of the instrument.
5.
Connect and secure the Drain Hose so that it will not move when hot pressurized fluid is exhausted.
Instrument Installation
Site Requirements
To install and operate the ANKOM
Adjustable wrench
Water supply located close to the ANKOM
70°C for ADF/NDF analyses
Adequate power (see “Operating Environment” section)
Drain
Instrument Installation Procedure
To install the ANKOM
2000
Fiber Analyzer, follow the procedure detailed below.
2000
Fiber Analyzer you will need the following:
2000
capable of heating water to 50°C for Crude Fiber and
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Operator’s Manual
6.
If you are using Cubetainers for your chemicals, install the Cubetainer Shelf shown below (Optional).
IMPORTANT
The solutions are gravity fed into the instrument ports. The bottom of the solution level
must be at least 5” (13cm) above the port. The top of the solution level can be no more
than 20” (50cm) above either port or the solution will overflow into the drain.
pg. 12 Rev E 1/14/15
Operator’s Manual
Item
Recommended Product
Electronic Balance with four-place readout
ANKOM #TB Balance Hardware
ANKOM #TBS Balance Software
Filter Bags
ANKOM #F57
Bag Holder (used for adding sample to an empty filter bag)
ANKOM #101.2
Heat Sealer for sealing the filter bags
ANKOM #1915 (120V), #1920 (220V)
Solvent Resistant Marker
ANKOM #F08
Desiccant Pouch
ANKOM #X45
Oven for drying (capable of maintaining 102°C ± 2°)
ANKOM #RD (120V), #RDI (220V)
Sample
-----------------
Spoon
-----------------
Fiber Analysis Support Items
The following support items are needed to perform the fiber analysis procedures:
Analysis Options using the ANKOM
The ANKOM
will run with default digestion and rinse time settings unless you select the Custom analysis option. This option
allows you to do ADF, NDF, or Crude Fiber analyses using custom digestion and rinse time settings.
For maintenance purposes, the ANKOM
The following sections provide the information you will need to use and maintain the ANKOM
2000
Fiber Analyzer can be configured to run ADF, NDF, and Crude Fiber analyses. The instrument
2000
also provides the capability to flush the solution lines.
Fiber Analyzer
2000
Fiber Analyzer.
2000
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Operator’s Manual
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pg. 14 Rev E 1/14/15
Operator’s Manual
ADF contained within a food or feed sample can be calculated using the following formula:
% ADF (as-received basis)
=
100 x (W3 – (W1 x C1))
W2
Where:
W1 = Bag tare weight
W2 = Sample weight
W3 = Dried weight of filter bag with fiber after extraction process
C1 = Blank bag correction (running average of final oven-dried weight
divided by original blank bag weight)
To prepare samples for fiber analysis, follow the procedure detailed below.
IMPORTANT
When using the ANKOM
2000
Fiber Analyzer for ADF analysis, at least one blank filter bag
should be included with the sample set as an indicator of particle loss. A running average
of the blank bag weights is used in the fiber calculation as the C1 correction factor. A C1
value larger than 1.0000 indicates that sample particles were lost from filter bags and
deposited on the blank bag. Any fiber particle loss from the filter bags will generate
erroneous results. If particle loss is observed, the grinding method for the specific sample
should be evaluated.
1.
Using a Solvent Resistant Marker, number all of the filter bags you will use
during the fiber analysis.
2.
Weigh and record the weight of each empty filter bag (W1).
3.
Set the Heat Sealer dial to between 4 and 5. (The setting may vary from sealer to
sealer.)
4.
Seal at least one empty filter bag (to be used as a blank)
within 4mm of its open end. Keep the sealer arm down for 2
– 3 seconds after the red sealer light turns off (to cool the
seal). The seal can be seen as a solid melted stripe along the
top edge of the filter bag (as shown to the right). If the seal
is not strong, re-seal the bag.
Seal
ADF Analysis
ADF Calculation
ADF Sample Preparation Procedure
Rev E 1/14/15 pg. 15
Operator’s Manual
5.
Place an empty filter bag in the Bag Holder in an open position.
6.
Tare the weight of the empty filter bag and the holder together.
7.
Add 0.45 – 0.50g of sample to the filter bag. Keep all particles away from the
sealing area of the filter bag.
8.
Record the weight of the sample (W2) and tare.
9.
Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red
sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of
the filter bag. If the seal is not strong, re-seal the bag.
10.
Spread the sample out uniformly within the filter bag by shaking and flicking the bag to eliminate
clumping.
11.
Repeat steps 5 – 10 for all filter bags that will be used in the Analyzer. (Up to 24 bags can be processed
during one procedure.)
IMPORTANT
If your samples contain soybean products or >5% fat, before doing the ADF analysis in
the ANKOM
2000
, you will need to do a pre-extraction. For samples containing non-roasted
soybean or >5% fat, follow the pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times and allow bags to soak for 10 minutes.
5. Pour out and dispose of the acetone.
6. Execute steps 1 through 5 a total of two times.
7. Place the bags on a wire screen to air-dry.
If your samples contain roasted soybean, follow the pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times.
5. Pour out and dispose of the acetone.
6. Pour fresh acetone into the container and allow the samples to soak for twelve
hours.
7. Pour out the acetone.
8. Place the bags on a wire screen to air-dry.
12.
Place the filter bags with sample and at least one empty bag (used as a Blank) into
the Bag Suspender trays as shown (maximum of three bags per tray).
13.
Stack each tray on the Bag Suspender rod (eight trays in total) with each tray
rotated 120 degrees from the tray below.
IMPORTANT
You must use all eight trays even if they are empty.
14.
Add the 9th tray to the top of the Bag Suspender rod. This tray contains no filter bags and acts as a cover.
The samples are now ready for the ADF analysis procedure.
pg. 16 Rev E 1/14/15
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