Agilent ZAG-110 Quick Start Guide

Analytical specifications

1,2

dsDNA 110 assay

DNA Sizing Range

35 bp – 5,000 bp

DNA Sizing Accuracy2

+ 5% or better

DNA Sizing Precision2

2% CV

DNA Fragment Concentration Range1

Physical Specifications

Total electrophoresis run time

33cm: 60 minutes, 55cm: 80 minutes

Samples per run

96-Capillary: 95 (+1 DNA Ladder Well) or 96 (Imported DNA Ladder)

Sample volume required

2 µL

Kit stability

4 months

Agilent ZAG-110 dsDNA Kit Quick Guide for ZAG DNA Analyzer Systems

The Agilent ZAG DNA Analyzer system is an automated capillary electrophoresis platform for scalable, flexible, fast, and reliable electrophoresis of DNA fragments.

This Quick Guide is intended for use with the Agilent ZAG DNA Analyzer systems only. The ZAG-110 dsDNA assay is designed for analyzing double-stranded DNA fragments from 35 to 5,000 basepair.

Specifications

0.5 ng/µL – 50 ng/µL input DNA (adjustable by dilution sample)

Separation Resolution

Results using DNA ladder in 1X TE buffer.

Results using DNA samples in 1X TE buffer.

35 – 100 bp < 10%, 100 – 1,000 bp < 5%, 1,000 – 5,000 bp ≤ 10% (33-55 array) 35 – 1,500 bp < 5%, 1,500 – 5,000 bp < 10% (55-80 array)

ZAG-110 dsDNA Quick Guide for ZAG DNA Analyzer Systems

concentration in 1X TE Buffer

Qualitative DNA, 1000/5000, RT

DNF

100mL

WARNING

•

Kit Components – 5000 Sample Kit

Kit Component Number

Part Number (Re-order Number)

Description Quantity Per Kit

5191-6609*

ZAG-110-FR*

5191-6615*

ZAG 115 dsDNA (35-5,000bp), 5000, 4oC

ZAG-110-0500 ZAG 110 dsDNA Separation Gel, 500mL 1

DNF-495-0125 Dilution Buffer 1x TE, 125mL 1

DNF-355-0500 5x 930 dsDNA Inlet Buffer, 500 mL

• Dilute with sub-micron filtered water prior to use

ZAG 110 dsDNA, FR

DNF-600-U030

FS-SLR915-0001

FA-SMK480-0003 35bp and 5,000bp Markers, 3.2mL 1

-475-0100

-SMO15

Intercalating Dye, 30 μL

100bp DNA Plus Ladder, 1mL

• 100 bp – 3,000 bp; 2.5ng/µL total DNA

• 0.5ng/µL concentration each in 1x TE

buffer

5x Capillary Conditioning Soln,

Mineral Oil Dropper Bottle, 15mL

*Not orderable

Refer to product safety data sheets for further information

When working with the ZAG DNA Analyzer kit components follow the appropriate safety procedures such as wearing goggles, safety gloves and protective clothing.

ZAG-110 dsDNA Quick Guide for ZAG DNA Analyzer Systems

Ambient operating temperature: 19 – 25 °C (66 – 77 °F)

Keep reagents during sample preparation at room temperature

Ensure that no sample or Diluent Marker remains within or on the outside of the tip

• Apply a new seal to 96-well sample plate prior to mixing and centrifugation

the well thoroughly to achieve the most accurate quantification. It is highly

Additional Material Required for Analysis with the ZAG DNA Analyzer Systems

• ZAG DNA Analyzer systems with LED fluorescence detection:

• ZAG DNA Analyzer system (p/n M5320AA)

• ZAG 96-Capillary Array Short, 33 cm (p/n A2300-9650-3355) OR

• ZAG 96-Capillary Array Long, 55 cm (p/n A2300-9650-5580):

• Agilent ZAG DNA Analyzer controller software (Version 1.0 or higher)

• Agilent ProSize data analysis software (Version 2.0.0.61 or higher)

Additional equipment/reagents required (not supplied)

• 96-well PCR sample plates. Please refer to Appendix – ZAG DNA Analyzer Compatible Plates and Tubes in the ZAG DNA Analyzer System User Manual for a complete approved sample plate list

• Multichannel pipettor(s) and/or liquid handling device capable of dispensing 1 – 100 µL volumes (sample plates) and 1,000 µL volumes (inlet buffer plate)

• Pipette tips

• 96-well plate centrifuge for spinning down bubbles from sample plates

• Sub-micron filtered DI water system, (for diluting the 5x 930 dsDNA Inlet Buffer and 5x Capillary Conditioning Solution

• 96-deepwell 1mL plate: Fisher Scientific #12-566-120 (inlet buffer and/or waste plate)

• Reagent reservoir, 50 mL (VWR #89094-680 or similar) (for use in pipetting inlet buffer plates/sample trays)

• Conical centrifuge tubes for prepared separation gel/dye mixture and/or 1x Capillary Conditioning Solution

• 250 mL conical: Corning #430776, available from Fisher Scientific #05-538-53 or VWR #21008-771

• Vortexer (for mixing of samples, ladders, and/or markers in tubes and/or plates)

Capillary Storage Solution (p/n GP-440-0100)

•

Essential Measurement Practices

Environmental conditions

Steps before sample preparation

Pipetting practice

Mixing and centrifugation recommendations

•

• Allow reagents to equilibrate at room temperature for 30 min prior to use

• Pipette reagents carefully against the side of the 96-well sample plate or sample

tube

•

• When mixing sample with Diluent Marker (DM), it is important to mix the contents of

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