Agilent ZAG-105 Quick Start Guide
Analytical specifications
1,2
dsDNA 105 assay
DNA Sizing Range
35 bp - 500 bp
DNA Sizing Accuracy2
+ 5% or better
DNA Sizing Precision2
2% CV
DNA Fragment Concentration Range1
Physical Specifications
Total electrophoresis run time
33cm: 60 minutes, 55cm: 80 minutes
Samples per run
96-Capillary: 95 (+1 DNA Ladder Well) or 96 (Imported DNA Ladder)
Sample volume required
2 µL
Kit stability
4 months
Agilent ZAG-105 dsDNA Kit Quick
Guide for ZAG DNA Analyzer
Systems
The Agilent ZAG DNA Analyzer system is an automated capillary electrophoresis platform for scalable,
flexible, fast, and reliable electrophoresis of DNA fragments.
This Quick Guide is intended for use with the Agilent ZAG DNA Analyzer system only. The ZAG-105 dsDNA kit is
designed for analyzing double-stranded DNA fragments from 35 to 500 base pair.
Specifications
0.5 ng/µL – 50 ng/µL input DNA (adjustable by dilution sample)
Separation Resolution
1
Results using DNA ladder in 1X TE buffer.
2
Results using DNA samples in 1X TE buffer.
5 – 10 bp @ 300 bp (33-55 array)
3 – 5 bp @ 300 bp (55-80 array)
1
ZAG-105 dsDNA Quick Guide for ZAG DNA Analyzer Systems
concentration in 1X TE Buffer
Qualitative DNA,
1000/5000, RT
DNF
100mL
FS
Bottle, 15mL
WARNING
•
•
Kit Components – 5000 Sample Kit
Kit Component
Number
Part Number
(Re-order Number)
Description Quantity Per Kit
5191-6608*
ZAG-105-FR*
5191-6615*
ZAG 105 dsDNA (1-500bp), 5000, 4°C
ZAG-105-0500 ZAG 105 dsDNA Separation Gel, 500mL 1
DNF-495-0125 Dilution Buffer 1x TE, 125mL 1
DNF-355-0500 5x 930 dsDNA Inlet Buffer, 500 mL
• Dilute with sub-micron filtered water prior
to use
ZAG 105 dsDNA, FR
DNF-600-U030
FS-SLR905-0001
FA-MRK900F-0003 Markers, 1bp & 500bp 10% Formamide , 3.2mL 1
-475-0100
Intercalating Dye, 30 μL
35-400bp DNA Ladder, 1mL
• 35 bp – 400 bp; 2ng/µL total DNA
• Lower Marker (set to 1 bp) and Upper
Marker (500 bp; at 0.5 ng/µL) in 1x TE
buffer with 10% Formamide
5x Capillary
Conditioning Soln,
1
1
2
2
-SMO15
*Not orderable
Mineral Oil Dropper
Refer to product safety data sheets for further information
When working with the ZAG DNA Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
1
2
ZAG-105 dsDNA Quick Guide for ZAG DNA Analyzer Systems
Ambient operating temperature: 19 – 25 °C (66 – 77 °F)
Ensure that no sample or Diluent Marker remains within or on the outside of the tip
• Apply a new seal to 96-well sample plate prior to mixing and centrifugation
suggested to perform one of the following methods to ensure complete mixing.
Additional Material Required for Analysis with the ZAG DNA Analyzer System
• ZAG DNA Analyzer system with LED fluorescence detection:
• ZAG DNA Analyzer system (p/n M5320AA)
• ZAG 96-Capillary Array Short, 33 cm (p/n A2300-9650-3355) OR
• ZAG 96-Capillary Array Long, 55 cm (p/n A2300-9650-5580):
• Agilent ZAG DNA Analyzer controller software (Version 1.0 or higher)
• Agilent ProSizeData Analysis software (Version 2.0.0.61 or higher)
Additional equipment/reagents required (not supplied)
• 96-well PCR sample plates. Please refer to Appendix – ZAG DNA Analyzer Compatible Plates and Tubes in the ZAG DNA
Analyzer System User Manual for a complete approved sample plate list
• Multichannel pipettor(s) and/or liquid handling device capable of dispensing 1 – 100 µL volumes (sample plates) and
1,000 µL volumes (inlet buffer plate)
• Pipette tips
• 96-well plate centrifuge (for spinning down bubbles from sample plates)
• Sub-micron filtered DI water system (for diluting the 5x 930 dsDNA Inlet Buffer and 5x Capillary Conditioning Solution)
• 96-deepwell 1mL plate: Fisher Scientific #12-566-120 (inlet buffer and/or waste plate)
• Reagent reservoir, 50 mL (VWR #89094-680 or similar) (for use in pipetting inlet buffer plates/sample trays)
• Conical centrifuge tubes for prepared separation gel/dye mixture and/or 1x Capillary Conditioning Solution
• 250 mL conical: Corning #430776, available from Fisher Scientific #05-538-53 or VWR #21008-771
• Vortexer (for mixing of samples, ladders, and/or markers in tubes and/or plates)
Capillary Storage Solution (p/n GP-440-0100)
•
Essential Measurement Practices
Environmental
conditions
Steps before
sample preparation
Pipetting practice
Mixing and
centrifugation
recommendations
•
• Keep reagents during sample preparation at room temperature
• Allow reagents to equilibrate at room temperature for 30 min prior to use
• Pipette reagents carefully against the side of the 96-well sample plate or sample
tube
•
• When mixing sample with Diluent Marker (DM), it is important to mix the contents of
the well thoroughly to achieve the most accurate quantification. It is highly
3
Loading...